1. Vesicular stomatitis virus matrix protein gene enhances the antitumor effects of radiation via induction of apoptosis
- Author
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Hong-bin Wu, Ju-mei Zhao, Ling-yu Fan, Hong-Xia Li, Jin-Yi Lang, Li Liu, Xia Zhao, You Lu, Bin Kan, Peng Diao, Yongsheng Wang, Yuquan Wei, Xiao-bo Du, Zhi-ping Yuan, Guo-Qing Wang, Jian-Rong Xu, Rui Wang, Han-Suo Yang, Yun-Qiu Mao, Hong-bo Wu, Zhenyu Ding, Bin Yao, Tao Li, and Yan-jun Wen
- Subjects
Cancer Research ,Myeloma protein ,Clinical Biochemistry ,Pharmaceutical Science ,Apoptosis ,Matrix (biology) ,Biology ,Fatty Acids, Monounsaturated ,Viral Matrix Proteins ,Mice ,In vivo ,Cell Line, Tumor ,Neoplasms ,Animals ,Tumor Stem Cell Assay ,Cell Proliferation ,Pharmacology ,Mice, Inbred BALB C ,Liposome ,Viral matrix protein ,Biochemistry (medical) ,Genetic Therapy ,Cell Biology ,biology.organism_classification ,Molecular biology ,In vitro ,Mice, Inbred C57BL ,Quaternary Ammonium Compounds ,stomatognathic diseases ,Gamma Rays ,Vesicular stomatitis virus ,Liposomes ,Cancer research - Abstract
Vesicular stomatitis virus (VSV) matrix (M) protein can directly induce apoptosis by inhibiting host gene expression when it is expressed in the absence of other viral components. Previously, we found that the M protein gene complexed to DOTAP-cholesterol liposome (Lip-MP) can suppress malignant tumor growth in vitro and in vivo; however, little is known regarding the biological effect of Lip-MP combined with radiation. The present study was designed to determine whether Lip-MP could enhance the antitumor activity of radiation. LLC cells treated with a combination of Lip-MP and radiation displayed apparently increased apoptosis compared with those treated with Lip-MP or radiation alone. Mice bearing LLC or Meth A tumors were treated with intratumoral or intravenous injections of Lip-MP and radiation. The combined treatment significantly reduced mean tumor volumes compared with either treatment alone in both tumor models and prolonged the survival time in Meth A tumor models and the intravenous injection group of LLC tumor models. Moreover, the antitumor effects of Lip-MP combined with radiation were greater than their additive effects when compared with the expected effects of the combined treatment in vivo. This study suggests that Lip-MP enhanced the antitumor activity of radiation by increasing the induction of apoptosis.
- Published
- 2008