Back to Search
Start Over
Humoral and cellular immunity induced by tumor cell vaccine based on the chicken xenogeneic homologous matrix metalloproteinase-2
- Source :
- Cancer Gene Therapy. 14:158-164
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Matrix metalloproteinase-2 (MMP-2) has been used as a target for cancer immunotherapy. The activation of immunization by breaking immune tolerance to self-MMP-2 may be one of the promising approaches for the treatment of MMP-2-positive tumors. In this study, we constructed the xenogeneic tumor cell vaccine c-MMP-2 by transfecting CT26 and LLC cells with chicken MMP-2 cDNA constructs. MMP-2-specific autoantibodies in sera and tumor cells were found in mice immunized with c-MMP-2. Protection against tumor growth was evaluated in respect of the relative contributions of autoantibodies, CD4+, and CD8+ T cells. Treatment with this vaccine (c-MMP-2) also prolonged the survival time of mice bearing cancer. The specific cytotoxic T-cell responses suggested that the treatment increased CD8+ T-cell activity. The antitumor activity of c-MMP-2 was abrogated by in vivo depletion of CD4+ and CD8+ T-lymphocytes and improved by adoptive transfer of CD4+ and CD8+ T-lymphocytes from the mice treated with c-MMP-2. An alternative DNA vaccination strategy for cancer therapy was identified in this study by eliciting humoral and cellular immunoresponse with a crossreacting transfectant.
- Subjects :
- Cancer Research
Cellular immunity
Adoptive cell transfer
DNA, Complementary
medicine.medical_treatment
Blotting, Western
Biology
Cancer Vaccines
Immune tolerance
DNA vaccination
Mice
Cancer immunotherapy
T-Lymphocyte Subsets
Cell Line, Tumor
Vaccines, DNA
medicine
Animals
Cytotoxic T cell
Molecular Biology
Autoantibodies
DNA Primers
Immunity, Cellular
Mice, Inbred BALB C
Base Sequence
Neovascularization, Pathologic
Reverse Transcriptase Polymerase Chain Reaction
Cancer
medicine.disease
Immunohistochemistry
Mice, Inbred C57BL
Antibody Formation
Colonic Neoplasms
Immunology
Matrix Metalloproteinase 2
Molecular Medicine
Chickens
CD8
Subjects
Details
- ISSN :
- 14765500 and 09291903
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Cancer Gene Therapy
- Accession number :
- edsair.doi.dedup.....f9d18764eb16884c86ee3d545f6596df