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Abstract Background Declining physical activity and increasing screen time (ST) among Chinese adolescents have become major concerns shared by scholars, while mental health issues are also on the rise. Previous studies have confirmed the association between physical activity and screen time and psychological symptoms, but it is unclear how their psychological symptoms, especially for Chinese university students who have a high proportion of psychological symptoms, and no research evidence has been found. Methods This study investigated physical activity, screen time, and psychological symptoms in 11,173 university students aged 19–22 years in six regions of China. A binary logistic regression analysis was used to analyze the association between moderate-to-vigorous physical activity (MVPA) and screen time and psychological symptoms. And the generalize linear model (GLM) analysis was used to further analyze the association between MVPA and screen time and psychological symptoms. Results The detection rate of psychological symptoms among Chinese university students was 16.3%, with a higher percentage of female students (17.5%) than male students (14.7%). The proportion of male students (8.2%) with MVPA > 60 min/d was higher than that of female students (2.3%), and the proportion of male students (33.8%) and female students (34.5%) with screen time > 2 h/d was basically the same. The generalize linear model (GLM) analysis showed that university students with MVPA 2 h/d (OR = 1.59, 95% CI: 1.10–2.31) had the highest risk of psychological symptoms (OR = 1.59, 95% CI: 1.10–2.31) compared to university students with MVPA > 60 min/d and screen time 2 h/d (OR = 1.59,95% CI: 1.10–2.31). In addition, university students with MVPA > 60 min/d and a screen time of 1–2 h/d (OR = 0.09, 95% CI: 0.03–0.25) had the lowest risk of psychological symptoms (P

Abstract Noise impairs the performance of inverse synthetic aperture radar (ISAR) motion compensation, which induces severe defocusing under low signal‐to‐noise ratio environments. To overcome this issue, a novel similarity‐oriented (SO) method with a two‐domain denoising strategy is proposed. A PIxEl similarity‐oriented (PIE‐SO) denoising method designed for range‐Doppler (RD) domain and a modified RAnge Profile Similarity‐Oriented (RAP‐SO) denoising method designed for high‐resolution range profile (HRRP) matrix are included in the presented framework. Firstly, the PIE‐SO method directly performs a two‐dimensional fast Fourier transform on dechirp processed echo data to form a coarsely focusing ISAR image in the RD domain. Then the focusing image is separated from the noise background by virtue of pixel similarity, after which the noise is preliminarily removed. Subsequently, the coarsely denoised image is transformed into the HRRP matrix. Considering the range profile similarity impaired by noise is restored by the PIE‐SO denoising, a Laplacian regularised‐weighted nuclear norm proximal (LR‐WNNP) operator is proposed. The proposed modified RAP‐SO method, that is, the LR‐WNNP operator, exploits the low‐rank property of the HRRP matrix and the local similarity of adjacent HRRPs to reduce the residual noise. As a result, ISAR imaging quality is significantly improved. Comprehensive experiments illustrate the effectiveness and superiority of the presented method.

BackgroundThe relationship between vegetarianism and mental well-being remains a debated topic in traditional observational studies. Recent studies have revealed the genetic factors in vegetarianism. We aimed to use genetic variations to explore the potential causal relationships between vegetarianism and mental well-being, offering insights from a new perspective.MethodsWe conducted the inverse variance weighted approach as the primary analysis to explore the bidirectional genetic associations between vegetarianism (N = 442,589) and depressive symptoms (N = 180,866), neuroticism (N = 170,910), and subjective well-being (N = 298,420). The analysis used the summary data from the largest genome-wide association studies (GWAS). We also performed sensitivity analyses to ensure the robustness of the findings, accounting for potential heterogeneity and pleiotropy.ResultsGenetically predicted vegetarianism showed positive causal relationships with depressive symptoms (odds ratio [OR], 3.26; 95% confidence interval [CI], 1.03–10.31; p = 0.044) and neuroticism (OR, 6.72; 95% CI, 2.29–19.74; p = 5.31 × 10−4), as well as a negative causal relationship with subjective well-being (OR, 0.20; 95% CI, 0.05–0.77; p = 0.019). Additionally, depressive symptoms were found to have a causal influence on vegetarianism (OR, 1.01; 95% CI, 1.00–1.02; p = 6.87 × 10−3). No significant heterogeneity or pleiotropy was detected.ConclusionVegetarianism is causally correlated with negative mental well-being, reflected in an increased risk of depressive symptoms and neuroticism, as well as lower subjective well-being. Further research should explore the underlying mechanisms in broader populations.

Abstract Background The Porcine Epidemic Diarrhea Virus (PEDV) has caused significant economic losses in the global swine industry. As a potential drug for treating diarrhea, the antiviral properties of attapulgite deserve further study. Methods In this study, various methods such as RT-qPCR, Western blot, viral titer assay, Cytopathic Effect, immunofluorescence analysis and transmission electron microscopy were used to detect the antiviral activity of attapulgite and to assess its inhibitory effect on PEDV. Results When exposed to the same amount of virus, there was a significant decrease in the expression of the S protein, resulting in a viral titer reduction from 10-5.613 TCID50/mL to 10-2.90 TCID50/mL, which represents a decrease of approximately 102.6 folds. Results of cytopathic effect and indirect immunofluorescence also indicate a notable decrease in viral infectivity after attapulgite treatment. Additionally, it was observed that modified materials after acidification had weaker antiviral efficacy compared to powdered samples that underwent ultrasonic disintegration, which showed the strongest antiviral effects. Conclusion As a result, Attapulgite powders can trap and adsorb viruses to inhibit PEDV in vitro, leading to loss of viral infectivity. This study provides new materials for the development of novel disinfectants and antiviral additives.

This study takes a nearby foundation pit project of a high-speed railway in Yancheng City, Jiangsu Province as an example to investigate the impact of deep foundation pit projects on groundwater and the surrounding environment. Through on-site monitoring, in-situ testing, and numerical simulation, we conducted a comprehensive study of the impact of foundation pit projects on groundwater and the surrounding environment. The evolution of groundwater levels and surface displacement during the excavation of foundation pits was analyzed, and a method for measuring the permeability of target soil layers in situ was studied. The simulation of recharge under various soil conditions was performed, and the outcomes demonstrated the effective isolation of hydraulic connections between the interior and exterior of the foundation pit by the water-resistant curtain. Precipitation head and constant head recharging tests were employed to ascertain the actual permeability. The characteristic time method calculation exhibited remarkable efficiency and accuracy in determining the permeability coefficient of the recharging soil layer. Recharging phreatic and confined water layers has differing degrees of influence on surface deformation, with the impact of confined water layers requiring more time to stabilize. These findings contribute significantly to a more comprehensive understanding of the environmental repercussions associated with deep excavation projects, thereby enhancing safety and environmental protection measures in excavation construction practices.

Myocardial infarction (MI) is a serious cardiovascular disease with a high mortality rate worldwide. Early detection and consistent treatment can significantly reduce mortality from cardiovascular diseases. However, there is a need for efficient models that can enable the early detection of heart disease without relying on trained clinical experts. MI studies using phonocardiogram (PCG) signals and implementing ensemble learning models are still relatively scarce, often resulting in poor accuracy and low detection rates. This study aims to implement an ensemble learning model for the classification of MI using PCG signals into different classes. In this stage of research, several classification algorithms, including Random Forest and Logistic Regression, serve as basic models for ensemble learning, utilizing features extracted from audio signals. Evaluation of the model's performance reveals that the stacking model achieves an accuracy of 96%. These results demonstrate that our system can appropriately and accurately classify MI within PCG data. We believe that the findings of this study will enhance the diagnosis and treatment of heart attacks, making them more effective and accurate.

This study investigated the physicochemical properties, functionalities, and antioxidant capacities of protein extracts from wild sea cucumber Australostichopus mollis collected from four distinct locations in New Zealand. Protein was extracted from sea cucumber body walls using trypsin enzymatic extraction, followed by cold acetone precipitation. The amino acid analysis revealed high glycine (189.08 mg/g), glutamic acid (119.45 mg/g), and aspartic acid (91.91 mg/g) concentrations in all samples. The essential amino acid indexes of the protein extracts (62.96, average) were higher than the WHO/FAO standard references, indicating the excellent protein quality of A. mollis. Furthermore, protein extracts from A. mollis demonstrated superior emulsifying activity (202.3–349.5 m2/g average) compared to commercial soy and whey protein isolates under all tested pH conditions, and enhanced foaming capacity (109.9–126.4%) and stability (52.7–72%) in neutral and acidic conditions. The extracts also exhibited good solubility, exceeding 70% across pH 3–11. Antioxidant capacities (ABTS and DPPH free radical scavenging activity and ferric reducing antioxidant power) were identified in A. mollis protein extracts for the first time, with clear variations observed among different locations. These findings elucidate the advantageous functional properties of protein extracts from wild New Zealand A. mollis and highlight their potential application as high-quality antioxidant food ingredients.

Abstract Nonalcoholic fatty liver disease (NAFLD) is one of major causes of end-stage liver disease in the coming decades, but it shows few symptoms until it develops into cirrhosis. We aim to develop classification models with machine learning to screen NAFLD patients among general adults. This study included 14,439 adults who took health examination. We developed classification models to classify subjects with or without NAFLD using decision tree, random forest (RF), extreme gradient boosting (XGBoost) and support vector machine (SVM). The classifier with SVM was showed the best performance with the highest accuracy (0.801), positive predictive value (PPV) (0.795), F1 score (0.795), Kappa score (0.508) and area under the precision-recall curve (AUPRC) (0.712), and the second top of area under receiver operating characteristic curve (AUROC) (0.850). The second-best classifier was RF model, which was showed the highest AUROC (0.852) and the second top of accuracy (0.789), PPV (0.782), F1 score (0.782), Kappa score (0.478) and AUPRC (0.708). In conclusion, the classifier with SVM is the best one to screen NAFLD in general population based on the results from physical examination and blood testing, followed by the classifier with RF. Those classifiers have a potential to screen NAFLD in general population for physician and primary care doctors, which could benefit to NAFLD patients from early diagnosis.

The GaoFen (GF)-5-02 satellite is one of the new generations of hyperspectral observation satellites launched by China in 2021. The directional polarimetric camera (DPC) is an optical sensor onboard the GF-5-02 satellite. The precipitable water vapor (PWV) is a key detection parameter of DPC. However, the existing PWV data developed using DPC data have significant errors due to the lack of the timely calibration of the two bands (865, 910 nm) of DPC used for PWV retrieval. In order to acquire DPC PWV data with smaller errors, a calibration method is developed for these two bands. The method consists of two parts: (1) calibrate the 865 nm band of the DPC using the cross-calibration method, (2) calibrate the 910 nm band of the DPC according to the calibrated 865 nm band of the DPC. This method effectively addresses the issue of the absence of a calibration method for the water vapor absorption band (910 nm) of the DPC. Regardless of whether AERONET PWV data or SuomiNET PWV data are used as the reference data, the accuracy of the DPC PWV data developed using calibrated DPC data is significantly superior to that of the DPC PWV data retrieved using data before recalibration. This means that the calibration method for the NIR bands of the DPC can effectively enhance the quality of DPC PWV data.

Introduction: Apolipoprotein E (ApoE) has been shown to be necessary for proper skeletal muscle regeneration. Consistent with this finding, single-cell RNA-sequencing analyses of skeletal muscle stem cells (MuSCs) revealed that Apoe is a top marker of quiescent MuSCs that is downregulated upon activation. The purpose of this study was to determine if muscle regeneration is altered in mice which harbor one of the three common human ApoE isoforms, referred to as ApoE2, E3 and E4.Methods: Histomorphometric analyses were employed to assess muscle regeneration in ApoE2, E3, and E4 mice after 14 days of recovery from barium chloride-induced muscle damage in vivo, and primary MuSCs were isolated to assess proliferation and differentiation of ApoE2, E3, and E4 MuSCs in vitro.Results: There was no difference in the basal skeletal muscle phenotype of ApoE isoforms as evaluated by section area, myofiber cross-sectional area (CSA), and myonuclear and MuSC abundance per fiber. Although there were no differences in fiber-type frequency in the soleus, Type IIa relative frequency was significantly lower in plantaris muscles of ApoE4 mice compared to ApoE3. Moreover, ApoE isoform did not influence muscle regeneration as assessed by fiber frequency, fiber CSA, and myonuclear and MuSC abundance. Finally, there were no differences in the proliferative capacity or myogenic differentiation potential of MuSCs between any ApoE isoform.Discussion: Collectively, these data indicate nominal effects of ApoE isoform on the ability of skeletal muscle to regenerate following injury or the in vitro MuSC phenotype.

Abstract Recently, the gut microbiome has emerged as a potent modulator of exercise‐induced systemic adaptation and appears to be crucial for mediating some of the benefits of exercise. This study builds upon previous evidence establishing a gut microbiome‐skeletal muscle axis, identifying exercise‐induced changes in microbiome composition. Metagenomics sequencing of fecal samples from non‐exercise‐trained controls or exercise‐trained mice was conducted. Biodiversity indices indicated exercise training did not change alpha diversity. However, there were notable differences in beta‐diversity between trained and untrained microbiomes. Exercise significantly increased the level of the bacterial species Muribaculaceae bacterium DSM 103720. Computation simulation of bacterial growth was used to predict metabolites that accumulate under in silico culture of exercise‐responsive bacteria. We identified acetate and succinate as potential gut microbial metabolites that are produced by Muribaculaceae bacterium, which were then administered to mice during a period of mechanical overload‐induced muscle hypertrophy. Although no differences were observed for the overall muscle growth response to succinate or acetate administration during the first 5 days of mechanical overload‐induced hypertrophy, acetate and succinate increased skeletal muscle mitochondrial respiration. When given as post‐biotics, succinate or acetate treatment may improve oxidative metabolism during muscle hypertrophy.

Background: The purpose was to investigate the content, construct, and criterion validity of muscle ultrasound in a mixed cohort of participants recovering from mild and critical COVID-19.Methods: A secondary analysis of a prospective cross-sectional study was conducted on data obtained from a battery of muscle and physical function assessments including a muscle biopsy and muscle ultrasonography (US). Rectus femoris (RF) muscle thickness (mT), quadricep complex (QC) mT, RF muscle cross-sectional area (CSA) using 2D freeform trace and estimated from Feret’s diameter, and RF echo intensity (EI) were assessed with US. Muscle fiber CSA, fiber type, protein content in muscle fibers, extracellular matrix content (ECM; wheat-germ agglutin), and percent area of collagen in ECM (picrosirius red) were examined from vastus lateralis muscle biopsies. Spearman rho correlations (r) were performed to assess validity of ultrasound parameters.Results: Thirty-three individuals participated including 11 patients surviving critical COVID-19, 15 individuals recovering from mild-COVID, and 7 controls. There were several significant correlations between RF mT, QC mT, RF CSA, and RF EI with age, comorbid burden, body-mass index, and measures of muscle strength, muscle power, and physical function (range r = 0.35–0.83). RF Feret’s CSA correlated to CSA of type II muscle fibers (r = 0.41, p = 0.022) and the average size of all muscle fibers (r = 0.39, p = 0.031). RF EI was correlated with collagen in muscle ECM (r = 0.53, p = 0.003) and protein content in muscle tissue (r = −0.52, p = 0.012).Conclusion: Muscle size and quality measured using US has moderate content and construct validity, and to lesser extent, fair to moderate criterion validity in a mixed cohort of individuals recovering from COVID. Muscle ultrasound quality (EI) appears to be sensitive at detecting muscle dysfunction as it is associated with strength, power, physical function, and collagen distribution in a mixed group of individuals recovering from COVID-19.

Melt flow ratio (MFR or I21/I2) was measured for dozens of polyethylene (PE) in the melt index (MI or I2) range of 1–10 for various types of PE. I21/I2 for samples of linear low density polyethylene (LLD) polymerized by a Ziegler-Natta catalyst (ZN-LLD) lie near I21/I2 = 25, whereas those for specimens of metallocene linear low density polyethylene (m-LLD) scatter close to I21/I2 = 18. The plot of I21/I2 vs I2 for LLD showed that I21/I2 is almost independent on molecular weight and short chain branching (SCB), but strongly dependent on molecular weight distribution (MWD). The broader MWD, the larger I21/I2. This rule basically holds for both low density polyethylene (LDPE) and high density polyethylene (HDPE). However, at a certain I2 around 6 a discontinuous jump of I21/I2 for HDPE samples exits, where I21/I2 for samples of I2 less than 6 lie near those of LDPE and I21/I2 for ones having I2 bigger than 6 disperse close to those of ZN-LLD. In addition, bimodal PE or PE with long chain branching has a bigger I21/I2 in some case. Further, the relationship between the viscosity and melt index was discussed, indicating that I21/I2 as well as MWD is characteristic of all types of PE. Consequently, I21/I2 can be used as a convenient and efficient approach to identifying the type of PE.

Abstract In order to mitigate global warming, the international communities actively explore low-carbon and green development methods. According to the Paris Agreement that came into effect in 2016, there will be a global stocktaking plan to carry out every 5 years from 2023 onwards. In September 2020, China proposed a "double carbon" target of carbon peaking before 2030 and carbon neutrality before 2060. Achieving carbon peaking and carbon neutrality goals requires accurate carbon emissions and carbon absorptions. China's existing carbon monitoring methods have insufficient detection accuracy, low spatial resolution, and narrow swath, which are difficult to meet the monitoring requirement of carbon sources and sinks monitoring. In order to meet the needs of carbon stocktaking and support the monitoring and supervision of carbon sources and sinks, it is recommended to make full use of the foundation of the existing satellites, improve the detection technical specifications of carbon sources and sinks monitoring measures, and build a multi-means and comprehensive, LEO-GEO orbit carbon monitoring satellite system to achieve higher precision, higher resolution and multi-dimensional carbon monitoring. On this basis, it is recommended to strengthen international cooperation, improve data sharing policy, actively participate in the development of carbon retrieval algorithm and the setting of international carbon monitoring standards, establish an independent and controllable global carbon monitoring and evaluation system, and contribute China's strength to the global realization of carbon peaking and carbon neutrality.

African swine fever (ASF) is an acute and severe disease transmitted among domestic pigs and wild boars. This disease is notorious for its high mortality rate and has caused great losses to the world’s pig industry in the past few years. After infection, pigs can develop symptoms such as high fever, inflammation, and acute hemorrhage, finally leading to death. African swine fever virus (ASFV) is the causal agent of ASF; it is a large DNA virus with 150–200 genes. Elucidating the functions of each gene could provide insightful information for developing prevention and control methods. Herein, to investigate the function of I267L, porcine alveolar macrophages (PAMs) infected with an I267L-deleted ASFV strain (named ∆I267L) and wild-type ASFV for 18 h and 36 h were taken for transcriptome sequencing (RNA-seq). The most distinct different gene that appeared at both 18 hpi (hours post-infection) and 36 hpi was F3; it is the key link between inflammation and coagulation cascades. KEGG analysis (Kyoto encyclopedia of genes and genomes analysis) revealed the complement and coagulation cascades were also significantly affected at 18 hpi. Genes associated with the immune response were also highly enriched with the deletion of I267L. RNA-seq results were validated through RT-qPCR. Further experiments confirmed that ASFV infection could suppress the induction of F3 through TNF-α, while I267L deletion partially impaired this suppression. These results suggest that I267L is a pathogenicity-associated gene that modulates the hemorrhages of ASF by suppressing F3 expression. This study provides new insights into the molecular mechanisms of ASFV pathogenicity and potential targets for ASFV prevention and control.

Melioidosis, a severe tropical illness caused by Burkholderia pseudomallei, poses significant treatment challenges due to limited therapeutic options and the absence of effective vaccines. The pathogen’s intrinsic resistance to numerous antibiotics and propensity to induce sepsis during acute infections further complicate management strategies. Thus, exploring alternative methods for prevention and treatment is crucial. Monoclonal antibodies (mAbs) have emerged as a promising strategy for the prevention and treatment of infectious diseases. This study focused on generating three mAbs (13F1, 14G11, and 15D9) targeting hemolysin-coregulated protein 1 (Hcp1), a protein involved in the type VI secretion system cluster 1 (T6SS1) of B. pseudomallei. Notably, pretreatment with 13F1 mAb significantly reduced the intracellular survival of B. pseudomallei and inhibited the formation of macrophage-derived multinucleated giant cells (MNGCs). This protective effect was also observed in vivo. We identified a sequence of amino acids (Asp95-Leu114) within Hcp1 as the likely binding site for 13F1 mAb. In summary, our findings reveal that 13F1 mAb counteracts infection by targeting Hcp1, offering potential new targets and insights for melioidosis prevention.

This paper presents a microfluidic multifunctional device, which can flexibly change the light input energy by using the effect of Electrowetting-on-Dielectric(EWOD). It can also realize multiple functions of variable aperture, variable optical attenuator and optical switch. The utility model has the advantages of simple structure, small volume, simple operation, wide application, flexibility and low cost. In this paper, the optical characteristics of the proposed microfluidic multifunctional device are analyzed and discussed, and the structure is optimized. The results show that the insertion loss of the proposed microfluidic multifunctional device is as small as only 0.014 dB. The aperture of the liquid variable aperture varies from 0 to 0.506 mm. In theory, the variable optical attenuator has a variable attenuation range of 0 ~100%, and the attenuation can reach 40.2 dB when the applied voltage is 91.1 V. The state microfluidic optical switch is " off" when there is no applied voltage and the state is " on" when the applied voltage is greater than 135 V.

Let n≥2n\ge 2 and Ω⊂Rn\Omega \subset {{\mathbb{R}}}^{n} be a bounded nontangentially accessible domain. In this article, the authors investigate (weighted) global gradient estimates for Dirichlet boundary value problems of second-order elliptic equations of divergence form with an elliptic symmetric part and a BMO antisymmetric part in Ω\Omega . More precisely, for any given p∈(2,∞)p\in \left(2,\infty ), the authors prove that a weak reverse Hölder inequality with exponent pp implies the global W1,p{W}^{1,p} estimate and the global weighted W1,q{W}^{1,q} estimate, with q∈[2,p]q\in \left[2,p] and some Muckenhoupt weights, of solutions to Dirichlet boundary value problems. As applications, the authors establish some global gradient estimates for solutions to Dirichlet boundary value problems of second-order elliptic equations of divergence form with small BMO{\rm{BMO}} symmetric part and small BMO{\rm{BMO}} antisymmetric part, respectively, on bounded Lipschitz domains, quasi-convex domains, Reifenberg flat domains, C1{C}^{1} domains, or (semi-)convex domains, in weighted Lebesgue spaces. Furthermore, as further applications, the authors obtain the global gradient estimate, respectively, in (weighted) Lorentz spaces, (Lorentz–)Morrey spaces, (Musielak–)Orlicz spaces, and variable Lebesgue spaces. Even on global gradient estimates in Lebesgue spaces, the results obtained in this article improve the known results via weakening the assumption on the coefficient matrix.

Abstract Background Nonalcoholic steatohepatitis (NASH) is one of the most common liver diseases and has no safe and effective drug for treatment. We have previously reported the function of blueberry, but the effective monomer and related molecular mechanism remain unclear. Methods The monomer of blueberry was examined by ultra performance liquid chromatography-mass spectrometry (UPLC-MS). The NASH cell model was constructed by exposing HepG2 cells to free fatty acids. The NASH mouse model was induced by a high-fat diet for 12 weeks. NASH cell and mouse models were treated with different concentrations of blueberry monomers. The molecular mechanism was studied by Oil Red O staining, ELISA, enzyme activity, haematoxylin–eosin (H&E) staining, immunohistochemistry, immunofluorescence, western blot, RNA sequencing, and qRT-PCR. Results We identified one of the main monomer of blueberry as tectorigenin (TEC). Cyanidin-3-O glucoside (C3G) and TEC could significantly inhibit the formation of lipid droplets in steatosis hepatocytes, and the effect of TEC on the formation of lipid droplets was significantly higher than that of C3G. TEC can promote cell proliferation and inhibit the release of inflammatory mediators in NASH cell model. Additionally, TEC administration provided a protective role against high-fat diets induced lipid damage, and suppressed lipid accumulation. In NASH mouse model, TEC can activate autophagy, inhibit pyroptosis and the release of inflammatory mediators. In NASH cell model, TEC inhibited pyroptosis by stimulating autophagy. Then, small RNA sequencing revealed that TEC up-regulated the expression of tRF-47-58ZZJQJYSWRYVMMV5BO (tRF-47). The knockdown of tRF-47 blunted the beneficial effects of TEC on NASH in vitro, including inhibition of autophagy, activation of pyroptosis and release of inflammatory factors. Similarly, suppression of tRF-47 promoted the lipid injury and lipid deposition in vivo. Conclusions These results demonstrated that tRF-47-mediated autophagy and pyroptosis plays a vital role in the function of TEC to treat NASH, suggesting that TEC may be a promising drug for the treatment of NASH.

In recent years, the non-petroleum production of light olefins has been the research focus of Fischer–Tropsch olefin synthesis (FTO). Iron-based catalysts have attracted much attention because of their low price, high catalytic activity, and wide temperature range. In this paper, traditional modification, hydrophobic modification, and amphiphobic modification of the catalyst are summarized and analyzed. It was found that traditional modification (changing the pore size and surface pH of the catalyst) will reduce the dispersion of Fe, change the active center of the catalyst, and improve the selectivity of light olefins (for example, SiO2: 32%). However, compared with functional methods, these traditional methods lead to poor stability and high carbon dioxide selectivity (for example, SiO2: 34%). Hydrophobic modification can inhibit the adsorption and retention of water molecules on the catalyst and reduce the local water pressure near the iron species in the nuclear layer, thus inhibiting the further formation of CO2 (for example, SiO2: 5%) of the WGSR. Amphiphobic modification can not only inhibit the WGSR, but also reduce the steric hindrance of the catalyst, increase the diffusion rate of olefins, and inhibit the reabsorption of olefins. Follow-up research should focus on these issues.

Abstract Eggerthella lenta is a normal human microflora that is anaerobic, non-sporulating, and Gram positive. However, an increasing number of studies have shown that it could also be an important pathogen for humans, even causing life-threatening infection under certain conditions. However, understanding its pathogenic mechanism and treatment options still need to be improved; more clinical data are needed to explore it further. In this article, we report a case of ceftizoxime-cured E. lenta bacteremia and review the recent literature to provide more clinical data for the diagnosis of E. lenta bacteremia. Our report suggests that the frequency of E. lenta bacteremia is increased in patients with hematologic or solid organ cancer, diabetes mellitus and also in those with appendicitis.

Abstract Chlamydia spp. are prevalent zoonotic pathogens that infect a wide variety of host species. Chlamydia abortus (C. abortus) infection in yaks has been reported in Gansu and Qinghai province, China. However, no data about C. abortus infection are available in yaks in Tibet, China. A total of 938 serum samples was collected from yaks in Tibet, China and specific antibodies against C. abortus were detected by the enzyme-linked immunosorbent assay (ELISA). The results showed that the overall seroprevalence of C. abortus in yaks was 104/938 (11.1 %, 95 % confidence interval [CI] 9.1–13.1). The prevalence in female and male yaks was 59/556 (10.6 %, 95 % CI 8.0-13.2) and 45/382 (11.8 %, 95 % CI 8.5–15.0), respectively with no significant difference (p > 0.05). The seroprevalence of antibodies to C. abortus in yaks ranged from 8.0 to 18.2 % among the six different areas, and the difference was also without statistical significance (p > 0.05). The prevalence among different age groups ranged from 7.0 to 15.9 %, with a higher prevalence among 1 to 2 years age category. The results demonstrate the presence of C. abortus infection in yaks in Tibet and may pose a risk for the general yak populations in addition to its potential impact on public health and the local Tibetan economy. To our knowledge, this is the first seroprevalence survey of C. abortus in yaks in Tibet, China.

Porcine epidemic diarrhoea virus (PEDV) is a member of the genus Alphacoronavirus in the family Coronaviridae. It causes acute watery diarrhoea and vomiting in piglets with high a mortality rate. Currently, the GII genotype, PEDV, possesses a high separation rate in wild strains and is usually reported in immunity failure cases, which indicates a need for a portable and sensitive detection method. Here, reverse transcription–recombinase aided amplification (RT-RAA) was combined with the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas12a system to establish a multiplexable, rapid and portable detection platform for PEDV. The CRISPR RNA (crRNA) against Spike (S) gene of GII PEDV specifically were added into the protocol. This system is suitable for different experimental conditions, including ultra-sensitive fluorescence, visual, UV light, or flow strip detection. Moreover, it exhibits high sensitivity and specificity and can detect at least 100 copies of the target gene in each reaction. The CRISPR/Cas12a detection platform requires less time and represents a rapid, reliable and practical tool for the rapid diagnosis of GII genotype PEDV.

The clinical investigation explored that early recognition and intervention are crucial for preventing clinical deterioration in patients in Intensive Care units (ICUs). Deterioration of patients is predictable and can be preventable if early risk factors are recognized and developed in the clinical setting. Timely detection of deterioration in ICU patients may also lead to better health management. In this paper, a new model was proposed based on Long Short-Term Memory-Recurrent Neural Network (LSTM-RNN) to predict deterioration of ICU patients. An optimisation model based on a modified genetic algorithm (GA) has also been proposed in this study to optimize the observation window, prediction window, and the number of neurons in hidden layers to increase accuracy, AUROC, and minimize test loss. The experimental results demonstrate that the prediction model proposed in this study acquired a significantly better classification performance compared with many other studies that used deep learning models in their works. Our proposed model was evaluated for two tasks: mortality and sudden transfer of patients to ICU. Our results show that the proposed model could predict deterioration before one hour of onset and outperforms other models. In this study, the proposed predictive model is implemented using the state-of-the-art graphical processing unit (GPU) virtual machine provided by Google Colaboratory. Moreover, the study uses a novel time-series approach, which is minute-by-minute. This novel approach enables the proposed model to obtain highly accurate results (i.e., an AUROC of 0.933 and an accuracy of 0.921). This study utilizes the individual and combined effectiveness of different types of variables (i.e., vital signs, laboratory measurements, GCS, and demographic data). In this study, data was extracted from MIMIC-III database. The ad-hoc frameworks proposed by previous studies can be improved by the novel and reliable prediction framework proposed in this research, which will result in predictions of more accurate performance. The proposed predictive model could reduce the required observation window (i.e., a reduction of 83%) for the prediction task while improving the performance. In fact, the proposed significant small size of observation window could obtain higher results which outperformed all previous works that utilize different sizes of observation window (i.e., 48 hours and 24 hours). Moreover, this research demonstrates the ability of the proposed predictive model to achieve accurate results (>80%) on `raw' data in an experimental work. This shows that the rule-based pre-processing of clinical features is unnecessary for deep learning predictive models.

PurposeTo explore the intervention effect of external counterpulsation (ECP) combined with high-intensity aerobic exercise (HIAT) on patients with coronary heart disease (CHD) after PCI.Methods124 patients with stable CHD after PCI admitted to our hospital from June 2018 to June 2021 were selected, and all patients were divided into control group and observation group using the random number table method. The control group received conventional treatment, The observation group received ECP combined with HIAT based on the control group. The cardiorespiratory function indexes, exercise endurance indexes, incidence of major cardiovascular adverse events (MACE), Barthel index of the two groups were observed.ResultsAfter intervention, METs max, VO2max, VO2max/kg, VO2max/HR, and PP, ED, AT, and Barthel score in both groups were significantly higher than before intervention, and patients in the observation group were significantly higher than those in the control group (P < 0.05). The incidence of MACE in the observation group (3.23%) was lower than in the control group (12.90%) (P < 0.05).ConclusionECP combined with HIAT can improve the cardiopulmonary function of patients with CHD after PCI, and improve exercise endurance, reduce the incidence of MACE, improve patients' ability of daily living.

Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that is extremely difficult to solubilize. In response to these limitations, this study presents an optimized method for the extraction of protein from the organic phase of TRIzol that allows for higher yield recovery of skeletal muscle protein compared with direct homogenization in a common protein lysis buffer. The presented method is inexpensive, simple and fast, requires no additional treatment of the protein pellet for dissolution, and is compatible with downstream western blot applications.

Abstract Background The purpose of this study is to analyze the influence of radiation therapy on survival in a historical cohort of 56 pediatric patients with head and neck rhabdomyosarcoma. Methods A historical cohort of 56 pediatric patients with head and neck rhabdomyosarcoma from June 1st, 2013 to June 30th, 2019 was chosen. Clinical data and follow up results were collected including all diagnosis, treatment and prognosis information. Overall survival (OS) and event free survival (EFS) as time-to-event distributions were estimated with Kaplan-Meier method, and univariate analysis was performed with log rank test to detect differences between groups. Multivariate analysis was performed to explore the risk factors for survival with Cox proportional hazard model. Results The media follow up time of all 56 patients was 31.8 months (range 3.5–74.6 months). There were 26 events during follow up, including 14 disease progressions and 12 relapses. The estimated 5-year OS of all patients was 69.9%, and the estimated 5-year EFS was 48.8%. Patients with radiation therapy as a component of the initial treatment plan had better 5-year OS and EFS compared with those without radiation therapy (OS 80.3% vs. 49.7%, p = 0.003 and EFS 63.9% vs. 21.9%, p 5 cm and non-initial radiation therapy were independent risk factors for OS in all patients, non-initial radiation therapy was an independent risk factor for EFS in all patients, and tumor size > 5 cm was an independent risk factor for OS in patients with events. Conclusions Radiation therapy as a component of initial treatment can improve the OS and EFS in pediatric head and neck rhabdomyosarcoma patients by enhancing local control, and non-initial radiation therapy is an independent risk factor for OS and EFS. Salvage radiation therapy still can improve OS in patients with disease progression and relapse. Tumor size > 5 cm is an independent risk factor for OS in pediatric HNRMS patients with or without disease progression/relapse.

To evaluate the short-term prognosis of pediatric hepatoblastoma (HB) in relation to excision repair cross-complementation gene 1 (ERCC1) C118T single nucleotide polymorphism (SNP) and VEGF expression. ERCC1 C118T SNP and VEGF expression were detected and investigated in 31 children with HB undergoing platinum-based chemotherapy, to analyze their relationship with short-term pediatric HB prognosis. CC (38.7%; 12/31), CT (35.5%; 11/31), and TT (25.8%; 8/31) ERCC1 C118T mutation types were identified. The Kaplan-Meier survival curve analysis showed that the CC group had a better short-term prognosis than the CT + TT group (p = 0.010). VEGF was overexpressed in 14 cases (45.2%) and underexpressed in 17 cases (54.8%). The Kaplan-Meier survival curve analysis showed that the high VEGF expression group showed poorer short-term prognosis than the lower VEGF expression group (p = 0.004). In this study, ERCC1 C118T SNP in children with HB was mainly found to be mutant type CT + TT. Compared to wild type CC, children with the mutant type CT + TT exhibited better treatment efficacy and remission with platinum-based chemotherapy as well as better survival rates. Moreover, the short-term prognosis of children with low VEGF expression was better than in those with high expression.

BackgroundHepatoblastoma (HB) is the most common malignant embryonic liver tumor type in children under 3 years of age. In the present study, the next generation sequencing (NGS) method was used to detect the genotype characteristics of HB and summarize the correlation between the common mutation genotypes noted in this disease and the clinical treatment and prognosis. The results may aid clinical prognosis and the successful application of targeted drugs.MethodsInitially, DNA was extracted from tumor tissue specimens and peripheral blood derived from 19 pediatric patients with HB. Subsequently, DNA panel and NGS methods were used to detect tumor diagnosis and the expression levels of treatment-associated genes, followed by the summary of genotype characteristics. In addition, in order to further assess the application of immunotherapy in HB, immunohistochemical detection of programmed cell death 1 ligand 1 (PDL1) was performed in combination with tumor mutation burden (TMB) and DNA mismatch repair status analysis. Furthermore, the clinical treatment effect and prognosis of the pediatric patients were statistically analyzed according to the characteristics of the genotype. Overall prognosis and prognostic analyses in different groups were performed by Kaplan-Meier and log-rank tests, respectively. Finally, expression validation and diagnostic analysis of commonly reported genes were performed in the GSE75271 dataset, which was obtained from the Gene Expression Omnibus (GEO) database.ResultsIn the present study, certain mutated genes, including nuclear factor erythroid 2-related factor 2 (NFE2L2), catenin β1 (CTNNB1), MYCN, tumor protein p53, axis inhibition protein 1 (AXIN1) and adenomatous polyposis coli (APC) were associated with the pathogenesis of HB. During TMB and DNA mismatch repair status analyses, pediatric patients had a low TMB. All of them did not present with microsatellite instability. The immunohistochemical results indicated lower expression levels of PDL1 in HB. The complete remission (CR) rate of pediatric patients in the gene abnormality group was lower than that of the non-reported disease-associated gene abnormality group. The 2-year overall survival rate and disease-free survival rate of 19 pediatric patients with HB were 72.1% and 42.4%, respectively. Receiver operating characteristic (ROC) analysis demonstrated that CTNNB1, NFE2L2, AXIN1, APC, MYCN and insulin growth factor 2 (IGF2) may be potential biomarkers that could be used for the diagnosis of HB.ConclusionThe genotype changes in HB were more common and the CR rate of the pediatric patients with an altered genotype was lower than that of pediatric patients without an altered genotype. In addition, pediatric patients with HB exhibited lower TMB compared with adult patients. Moreover, the data indicated that CTNNB1, NFE2L2, AXIN1, APC, MYCN and IGF2 may be potential biomarkers that can be used for the diagnosis of HB.

Summary: Skeletal muscle is composed of post-mitotic myofibers that form a syncytium containing hundreds of myonuclei. Using a progressive exercise training model in the mouse and single nucleus RNA-sequencing (snRNA-seq) for high-resolution characterization of myonuclear transcription, we show myonuclear functional specialization in muscle. After 4 weeks of exercise training, snRNA-seq reveals that resident muscle stem cells, or satellite cells, are activated with acute exercise but demonstrate limited lineage progression while contributing to muscle adaptation. In the absence of satellite cells, a portion of nuclei demonstrates divergent transcriptional dynamics associated with mixed-fate identities compared with satellite cell replete muscles. These data provide a compendium of information about how satellite cells influence myonuclear transcription in response to exercise.

Attention Deficit Hyperactivity Disorder (ADHD) is a neurodevelopment disorder which causes inattention, hyperactivity, or impulsivity, that directly leads to the learning disability. The conventional ADHD assessment method such as behavioural rating scale requires long term behavior observation and subjective interpretation which may lead to misleading result. Over the past decades, there have been exponential growth in the research in biomedical engineering perspective such as Electroencephalograph (EEG), Magnetic Resonance Imaging (MRI) and electrodermal responding to identify accurate and objective measurements in ADHD assessment. Nevertheless, a clear definitive picture which link neuropsychological, behavioral, and neurophysiological research has yet to develop. The purpose of this study is to investigate ADHD in children aged from 7 to 14 years old at primary schools in Malaysia using Electro Interstitial Scan (EIS) technology which based on chronoamperometry technique. There are total of 182 children data have been collected, which consist of 58 ADHD children (study group) and 124 healthy children (control group). All subjects will undergo EIS measurement and the SPSS statistical analysis was conducted on the primary and secondary EIS parameters to identify significant level in differentiating ADHD and non-ADHD children. The statistical analysis result shows that EIS system reflects significant difference between control group and study group for both primary parameters (EIS channels 1, 2, 3, 4, 9, 10, 15, 16, 17 and 18) and secondary parameters (brain and neurotransmitter related parameters) (p

Microsporidia are obligate intracellular parasites that infect a wide variety of hosts ranging from invertebrates to vertebrates. These parasites have evolved strategies to directly hijack host mitochondria for manipulating host metabolism and immunity. However, the mechanism of microsporidia interacting with host mitochondria is unclear. In the present study, we show that microsporidian Encephalitozoon greatly induce host mitochondrial fragmentation (HMF) in multiple cells. We then reveal that the parasites promote the phosphorylation of dynamin 1-like protein (DRP1) at the 616th serine (Ser616), and dephosphorylation of the 637th serine (Ser637) by highly activating mitochondrial phosphoglycerate mutase 5 (PGAM5). These phosphorylation modifications result in the translocation of DRP1 from cytosol to the mitochondrial outer membrane, and finally lead to HMF. Furthermore, treatment with mitochondrial division inhibitor 1 (Mdivi1) significantly reduced microsporidian proliferation, indicating that the HMF are crucial for microsporidian replication. In summary, our findings reveal the mechanism that microsporidia manipulate HMF and provide references for further understanding the interactions between these ubiquitous pathogens with host mitochondria.

Foot-and-mouth disease (FMD) is a highly contagious disease that affects cloven-hoofed animals such as pigs, cattle, and sheep. The disease is caused by the foot-and-mouth disease virus (FMDV) which has a non-enveloped virion with icosahedral symmetry that encapsulates a positive-sense, single-stranded RNA genome of ∼8.4 kb. FMDV infection causes obvious immunosuppressive effects on the host. In recent years, studies on the immunosuppressive mechanism of FMDV have become a popular topic. In addition, studies have shown that many FMDV proteins are involved in the regulation of host innate immunity and have revealed mechanisms by which FMDV proteins mediate host innate immunity. In this review, advances in studies on the mechanisms of interaction between FMDV proteins and host innate immunity are summarized to provide a comprehensive understanding of FMDV pathogenesis and the theoretical basis for FMD prevention and control.

Objective To identify the drop-off location of victims in drowning cases, and confirm whether it is a fatal drowning or the victim is thrown into the water after death by detecting part of 5.8S sequence and second internal transcribed spacer （ITS2） （5.8S+ITS2） of diatom rDNA in water and organs. Methods Two cases identified by diatom examination, which received by Nanjing Municipal Public Security Bureau Forensic Center, were taken as the research objects. The difference of the population structure of algae in water and human tissue was analysed by length polymorphism of 5.8S+ITS2 marker. Results In case 1, similar species of diatom were detected from victim’s lung and liver tissues and the water sample. Two kinds of DNA fragments with length of 330 bp and 376 bp were detected from victim’s lung tissue and the water sample using 5.8S+ITS2 marker, which could confirm the victim was drowning before death. In case 2, there was no diatom found in victim’s lung and liver tissues. Only one kind of DNA fragment with length of 331 bp and low relative fluorescence unit （RFU） was obtained from victim’s lung tissue using 5.8S+ITS2 marker, thus the victim was thrown into the water after death. Conclusion The experimental results of the two cases in present study are consistent with the actual facts and the result of the diatom microscopic examination. The difference of population structure of specific microorganism in water and human tissue can be detected by 5.8S+ITS2 marker, which can help to identify the drop-off location of victims in drowning cases, and confirm whether it is a fatal drowning or the victim is thrown into the water after death.

Abstract Background The tetracycline-responsive system (Tet-ON/OFF) has proven to be a valuable tool for manipulating gene expression in an inducible, temporal, and tissue-specific manner. The purpose of this study was to create and characterize a new transgenic mouse strain utilizing the human skeletal muscle α-actin (HSA) promoter to drive skeletal muscle-specific expression of the reverse tetracycline transactivator (rtTA) gene which we have designated as the HSA-rtTA mouse. Methods To confirm the HSA-rtTA mouse was capable of driving skeletal muscle-specific expression, we crossed the HSA-rtTA mouse with the tetracycline-responsive histone H2B-green fluorescent protein (H2B-GFP) transgenic mouse in order to label myonuclei. Results Reverse transcription-PCR confirmed skeletal muscle-specific expression of rtTA mRNA, while single-fiber analysis showed highly effective GFP labeling of myonuclei in both fast- and slow-twitch skeletal muscles. Pax7 immunohistochemistry of skeletal muscle cross-sections revealed no appreciable GFP expression in satellite cells. Conclusions The HSA-rtTA transgenic mouse allows for robust, specific, and inducible gene expression across muscles of different fiber types. The HSA-rtTA mouse provides a powerful tool to manipulate gene expression in skeletal muscle.

Abstract Background Pax7+ satellite cells are required for skeletal muscle fiber growth during post-natal development in mice. Satellite cell-mediated myonuclear accretion also appears to persist into early adulthood. Given the important role of satellite cells during muscle development, we hypothesized that the necessity of satellite cells for adaptation to an imposed hypertrophic stimulus depends on maturational age. Methods Pax7CreER-R26RDTA mice were treated for 5 days with vehicle (satellite cell-replete, SC+) or tamoxifen (satellite cell-depleted, SC-) at 2 months (young) and 4 months (mature) of age. Following a 2-week washout, mice were subjected to sham surgery or 10 day synergist ablation overload of the plantaris (n = 6–9 per group). The surgical approach minimized regeneration, de novo fiber formation, and fiber splitting while promoting muscle fiber growth. Satellite cell density (Pax7+ cells/fiber), embryonic myosin heavy chain expression (eMyHC), and muscle fiber cross sectional area (CSA) were evaluated via immunohistochemistry. Myonuclei (myonuclei/100 mm) were counted on isolated single muscle fibers. Results Tamoxifen treatment depleted satellite cells by ≥90% and prevented myonuclear accretion with overload in young and mature mice (p

Background: Lots of trials demonstrate that second-generation drug-eluting stents (G2-DES), with their improved properties, offer significantly superior efficacy and safety profiles compared to first generation DES (G1-DES) for patients with coronary artery disease (CAD) receiving percutaneous coronary intervention (PCI). This study aimed to verify the advantage of G2-DES over G1-DES in Chinese patients with stable CAD (SCAD). Methods: For this retrospective observational analysis, 2709 SCAD patients with either G1-DES (n = 863) or G2-DES (n = 1846) were enrolled consecutively throughout 2013. Propensity score matching (PSM) was applied to control differing baseline factors. Two-year outcomes, including major adverse coronary events as well as individual events, including target vessel-related myocardial infarction, target lesion revascularization (TLR), target vessel revascularization, and cardiogenic death were evaluated. Results: The incidence of revascularization between G1- and G2-DES showed a trend of significant difference with a threshold P - value (8.6% vs. 6.7%, χ2 = 2.995, P = 0.084). G2-DES significantly improved TLR-free survival compared to G1-DES (96.6% vs. 97.9%, P = 0.049) and revascularization-free survival curve showed a trend of improvement of G2-DES (92.0% vs. 93.8%, P = 0.082). These differences diminished after PSM. Multivariate Cox proportional hazard regression analysis showed a trend for G1-associated increase in revascularization (hazard ratio: 1.28, 95% confidence interval: 0.95–1.72, P = 0.099) while no significance was found after PSM. Other endpoints showed no significant differences after multivariate adjustment regardless of PSM. Conclusions: G1-DES showed the same safety as G2-DES in this large Chinese cohort of real-world patients. However, G2-DES improved TLR-free survival of SCAD patients 2 years after PCI. The advantage was influenced by baseline clinical factors. G1-DES was associated with a trend of increase in revascularization risk and was not an independent predictor of worse medium-term prognosis compared with G2-DES.