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A facile and rapid postsynthetic modification strategy for functionalization of covalent organic framework (COF) was developed to synthesize a tailor-made pH-responsive COF called TpPa-1@Au@GSH for highly efficient extraction of N1-methyladenosine (m1A). Glutathione (GSH) was judiciously designed as the functional group for extracting and releasing m1A by pH variations. With the aid of gold nanoparticles (Au NPs) as linkers, GSH was successfully introduced to the robust substrate TpPa-1 in only one step spending only 1 h. Owing to the several-to-one immobilization of GSH on Au NPs and the large surface area of TpPa-1, this functional COF was constructed with abundant m1A binding sites. TpPa-1@Au@GSH showed excellent selectivity for m1A extraction by capturing m1A from a mixture of 14 nucleoside analogues followed by mass spectrometry analysis. It was proved to have ultrafast adsorption ability (only 1 min incubation time), high binding capacity (5 mg g-1, m1A/TpPa-1@Au@GSH), good reusability (at least 5 times), and good storage stability (at least 8 months at room temperature). Great performance was also achieved in extracting m1A from both animal and plant biological samples. The adsorption mechanism was demonstrated to be based on the electrostatic interaction. This work proposed a new approach for m1A extraction, demonstrated the high potential of COFs in biological sample pretreatment, and offered an effective and versatile route for functionalization of COFs.

Strong ocean current influences a marine seismic survey and forces the streamer off-course from the survey line. The sideway drift of the streamer results in that the reflection data are no longer distributed in common midpoint gathers along the survey line but become swath distribution on one side of the ship track. This effect is known as “streamer feathering” which degrades the profile image of the 2D processed seismic data. However, if we have long streamer or closely spaced parallel 2D seismic survey lines, we may turn this deleterious effect into a good opportunity to generate 3D seismic volumes with swath distributed reflection data. We present two case studies in which 2D seismic data were collected offshore eastern Taiwan where the strong Kuroshio Current heavily influenced the ship speed and caused large streamer feathering. The first case is a large-offset 2D seismic profiling data collected using a 6-km long streamer. We processed the swath part of the reflection data in 3D that not only avoids the inappropriate smearing effect in 2D data processing but also generates a 3D seismic volume to help the seismic interpretation. In the second case, we adjusted our 2D survey strategy when realizing that strong Kuroshio Current was causing significant streamer feathering, and collected a set of closely spaced parallel 2D seismic lines. This multi-swath dataset covers a broad area which enables us to generate a 3D seismic volume. Since our datasets are not real 3D seismic data, we have tailored our processing flows to deal with different data configurations and limitations of each dataset. Our results show that not only we have enhanced 2D seismic images of the originally-interested survey lines, but also provide information on 3D geometry of the geological features imaged. The benefits and limitations of utilizing the streamer feathering effect to generate 3D seismic volumes from 2D seismic profile data are reported. Overall, this approach is a considerable way to handle 2D seismic data with large streamer feathering for both avoiding unreliable 2D seismic images and obtaining information on 3D geometry of the geological features imaged.

The development of facilely synthetic materials for highly efficient enrichment of N-linked glycopeptides is essential in glycoproteome analysis. In this work, by utilizing the self-assembling of glutathione (GSH) on silver nanoparticles (Ag NPs), and the formation and dispersion of Ag NPs on a robust TpPa-1 substrate, a newly functionalized covalent organic framework (COF) called TpPa-1@Ag@GSH was synthesized via a simple two step post-synthetic modification. TpPa-1@Ag@GSH and intermediate products were confirmed and evaluated by nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy-energy dispersive spectroscopy, Brunauer-Emmett-Teller and thermogravimetric analyses. Benefiting from the judicious selection of the substrate, the abundance of binding sites, relatively high affinity between GSH and N-linked glycopeptides, and the multivalent interactions between N-linked glycopeptides and unoccupied surfaces of Ag NPs, this porous material showed great performance in N-linked glycopeptide enrichment. By enriching N-linked glycopeptides in tryptic digests of human serum immunoglobulin G (human IgG) followed by mass spectrometry analysis, our method was proved to have good sensitivity (1 fmol), high selectivity (1 : 1500, human IgG to bovine serum albumin), high binding capacity (160 mg g-1, IgG/TpPa-1@Ag@GSH), ultra-fast capture ability (only 1 min incubation time), and good reusability (at least 5 times). It was also successfully applied to the enrichment of N-linked glycopeptides from complex biological samples. Our work improved the enrichment selectivity of COFs, reached the most rapid capture ability among off-column enrichment materials, and provided a very facile and easily popularized post-synthetic modification route for COFs in glycoproteome analysis.

In this study, we compiled recently collected (from 2005 to 2015) and previously reported (published and open access) gravity data, including land, shipborne and satellite-derived data, for Taiwan and its surrounding regions. Based on the cross-over error analysis, all data were adjusted; and, new Free-air gravity anomalies were obtained, shedding light on the tectonics of the region. To obtain the Bouguer gravity anomalies, the densities of land terrain and marine sediments were assumed to be 2.53 and 1.80 g/cm3, respectively. The updated gravity dataset was gridded with a spacing of one arc-minute. Several previously unnoticed gravity features are revealed by the new maps and can be used in a broad range of applications: (1) An isolated gravity high is located between the Shoushan and the Kaoping Canyon off southwest Taiwan. (2) Along the Luzon Arc, both Free-air and Bouguer gravity anomaly maps reveal a significant gravity discontinuity feature at the latitude of 21°20′N. (3) In the southwestern Okinawa Trough, the NE-SW trending cross-back-arc volcanic trail (CBVT) marks the low-high gravity anomaly (both Free-air and Bouguer) boundary.

Based on the multi-channel seismic (MCS) and gravity data offshore north of Taiping Island (Itu Aba Island) in the Spratly Islands (Nansha Islands), we revisited the crustal structures in the northern part of the southern margin of the Southwest (SW) Sub-basin of the South China Sea (SCS). The MCS data suggest that the basement structural highs in the southwest margin of the SCS are dominated by both fault blocks and volcanic basement structures that probably formed along with the basement faults. The gravity modeling results reveal that these volcanic basement structures were probably associated with the high velocity or density lower crust (HVDLC), suggesting that the southern margin of the SW Sub-basin serves as an intermediate margin. Based on the tectonic features in the SCS margins, including (1) the transition between the magma-poor margins of the Northwest (NW) Sub-basin and intermediate margins of the East and SW Sub-basins; (2) the cessation of the seafloor spreading in the NW Sub-basin being succeeded by a major ridge jump event, which was very likely to reflect an active mantle upwelling event; (3) the extensive distribution of the HVDLC beneath in the well-investigated northern margin of the East Sub-basin and as explored in the SW Sub-basin in this study; and (4) basement faulting and volcanism shown in this study that may be related to the HVDLC and hyper-extension, we proposed a conceptual model to interpret the origin of the HVDLC in the southern margin of the SW Sub-basin. The margin of the SCS basin was magma-poor at the time it occurred. An active mantle upwelling event caused by small-scale mantle convection, possibly related to the influence of the nascent Hainan mantle plume, was formed subsequently, resulting in a southward ridge jump and the HVDLC beneath the current margins of the East and SW Sub-basins. The East and SW Sub-basins continued to spread, latitudinally dividing their HVDLCs as the margins separated accordingly. Afterwards, the hyper-extension in the distal margins may also be responsible for a crust weakening zone wherein the subsequent volcanic events could occur.

Taiping Island, also known as Itu Aba, is the largest natural terrestrial landmass in the South China Sea and is centrally located. Using bathymetry and marine multi-channel seismic data, we explored the seismic stratigraphic features of the offshore and isolated carbonate platform north of Taiping Island. The western flank of the carbonate platform is characterized by an intercalation between high-amplitude and low-amplitude reflections, showing the landward and seaward migration of the platform foreslope deposits. In addition, there are two offshore carbonate build-ups that are underlain by normal faults. Six sequence boundaries and five depositional sequences caused by eustatic sea level cycles are identified and correlated with the eustatic sea level change chart. Although the evolution of the seismic sequences is partly controlled by local tectonics, the overall stacking pattern of the sedimentary strata in our study area reveals five third-order cycles and one second-order cycle, which is in accordance with the eustatic sea level chart. Additionally, the formations of the Western Taiping Seamount Group and the Zhenghe-Daoming Trough are preliminarily analyzed based on seismic data.

The role of NCAM in corticogenesis is incompletely understood. The authors demonstrate that NCAM controls NPC proliferation and fate decision through profilin2-dependent regulation of actin polymerization. This finding sheds new light on NCAM’s functions in neurodevelopmental and mental disorders., The development of cerebral cortex requires spatially and temporally orchestrated proliferation, migration, and differentiation of neural progenitor cells (NPCs). The molecular mechanisms underlying cortical development are, however, not fully understood. The neural cell adhesion molecule (NCAM) has been suggested to play a role in corticogenesis. Here we show that NCAM is dynamically expressed in the developing cortex. NCAM expression in NPCs is highest in the neurogenic period and declines during the gliogenic period. In mice bearing an NPC-specific NCAM deletion, proliferation of NPCs is reduced, and production of cortical neurons is delayed, while formation of cortical glia is advanced. Mechanistically, NCAM enhances actin polymerization in NPCs by interacting with actin-associated protein profilin2. NCAM-dependent regulation of NPCs is blocked by mutations in the profilin2 binding site. Thus, NCAM plays an essential role in NPC proliferation and fate decision during cortical development by regulating profilin2-dependent actin polymerization.

A facile and rapid postsynthetic modification strategy for functionalization of covalent organic framework (COF) was developed to synthesize a tailor-made pH-responsive COF called TpPa-1@Au@GSH for highly efficient extraction of

The enrichment of glycopeptides plays an important role in glycoproteomics. In this paper, a covalent-organic framework called TpPa-1, synthesized by the Schiff base reaction of 1,3,5-triformylphloroglucinol and paraphenylenediamine, was first successfully utilized as a hydrophilic porous material for N-linked glycopeptide enrichment. Using this material, interference from non-glycopeptides could be efficiently eliminated, which facilitated the mass spectrometry detection of glycopeptides. By capturing N-linked glycopeptides from tryptic digests of human IgG, our method was proved to have high sensitivity at the femtomole level. And it showed superior selectivity for glycopeptides even when non-glycopeptides were 1000 times more concentrated. Due to the strong covalent bonds, this material possessed good stability and could be repeatedly used for at least 10 times. The ultra-low mass density and abundant binding sites also provided it with high binding capacity (178 mg g−1, IgG/TpPa-1). Moreover, N-linked glycopeptides were easily enriched by this material from only 10 μL human serum, which demonstrated its potential in pretreatment of complex biological samples.

There is an urgent need among the scientific community for a better understanding of the air-sea interactions that occur during typhoons and improved accuracy in typhoon forecasting. To this end, the Institute of Oceanography at the National Taiwan University (IONTU) has developed a buoy that can measure meteorological and hydrographic conditions, and transmit the high-precision data in near real-time. The buoy captures various types of meteorological data, including air temperature, air pressure, relative humidity, wind speed and direction, and solar radiation. Additionally, the buoy measures key hydrographic data such as the temperature and salinity profiles of the upper 500-m water layer. Such prototype buoys were deployed in the western North Pacific, the world's most active region for typhoons, in the summers of 2015 and 2016. The buoys were moored at distances of approximately 375 km (station NTU1) and 175 km (station NTU2) from the southernmost tip of Taiwan. In 2015, NTU1 survived four typhoons (Linfa, Chanhom, Soudelor, and Goni) and successfully captured many crucial aspects of their behavior and evolution. Unfortunately, the prototype was damaged by two Category 4 super-typhoons, Soudelor and Goni. After the 2015 trial, the IONTU buoy team modified several parts that were destroyed in 2015 and added more instruments and devices to the NTU's prototype buoys, and the two modified buoys were used in 2016. These buoys survived five typhoons (Nepartak, Meranti, Malakas, Megi, and Aere) and successfully collected important data on all five of them. These trial results, as well as all observation data gathered from the two years, are presented in this paper.

For a better understanding of the air-sea interactions that occur during typhoons and improved accuracy in typhoon forecasting, the Institute of Oceanography at the National Taiwan University has developed a buoy that can measure meteorological and hydrographic conditions, and transmit the high-precision data in near real-time. The buoy captures various types of meteorological data, including air temperature, air pressure, relative humidity, wind speed and direction, rainfall, and solar radiation. Additionally, the buoy measures key hydrographic data, such as the temperature and salinity profiles of the upper 500-m water layer. The newly designed of buoy system is a low-power-consumption system and it can be in sleep mode between successive samplings to save power energy. The buoy is supplied electric power by lithium batteries and it can support buoy operation for more than 18 months. The sampling interval and data transmission rate are adjustable via Iridium satellite or UHF radio communications. When a typhoon approaching buoy, the operator can transmit a command to buoy via Internet/Iridium satellite communications to change data transmission rate from standard mode to intensive mode. In addition, the raw data can be retrieved through UHF radio communication between the buoy and a nearby ship. A prototype and two improved buoys were deployed off southeastern Taiwan for trial in summers of 2015 and 2016, respectively. These buoys survived nine typhoons and successfully collected important data on all nine of them. The detail of buoy system and mooring design are presented in this paper.

Bottom simulating reflectors (BSRs), high methane flux, shallow sulfide/methane interfaces, fluids venting from the seafloor, authigenic carbonates within sediments, methane reefs, and self-biomes are common seafloor features in the area off southwest Taiwan. The geophysical and geochemical signatures of these features suggest a high potential for gas hydrate (GH) reservoirs in the region. The BSRs are typically interpreted as the boundary between free gases and solid hydrate, whereas the upper reaches of the hydrate stability zone and the distribution of gas hydrate in shallow sediments are not well understood. This study shows the first results of a marine controlled-source electromagnetic survey, conducted in the offshore area of SW Taiwan in 2010. The survey aimed to provide electrical resistivity information of the shallow sediments. Three target areas were surveyed: (1) an area to the southeast of the Xiaoliuchiu Island (gas seep G96), (2) an area in the west of the Yung-An Ridge (YAR) and (3) an area in the northwest of the Good Weather Ridge (GWR). In total, fourteen survey lines with a total length of 72 km were completed. Our preliminary results show that relatively high resistivity anomalies occur within pockmarks and at gas seepage sites. The apparent resistivity is estimated to be about 1 Ohm-m higher than background in G96 and YAR sites, while an anomaly up to 2 Ohm-m is found in the GWR. At gas seep site G96, the high resistivity anomaly may be due to the existence of authigenic carbonates; whereas, the high resistivity anomaly in the NW of the GWR site may also be due to the existence of gas hydrate in the shallow seabed. Based on the resistivity anomaly, the gas hydrate saturation is about 16% in the shallow sediments below the pockmark area in the northwest side of the GWR site.

In the wireless sensor network routing protocol design, the sensor node monitoring data in multi-hop relay approach to sink node for transmission. For sink aggregation node fixed network, the nearby sink nodes of sensor nodes need to transfer other nodes of the monitoring data, consequently, it consumes large amounts of energy, and it's easy to premature death, making the network connectivity decline, even making the network intersected, shorten the lifetime of the network. In order to solve this problem, from the view of sink node, this thesis brings forward a protocol of SERP, which is a energy balancing routing protocol based on sink Mobility and prolongs the lifetime of network. It adopts the strategy of sink movement which makes the hot nodes inside the network take turns, balances the load among them. In SERP, firstly, the WSN deployment area is divided into finite Virtual cells ;Secondly ,we make the center of each virtual cell as the mobile position of sink node; at last, we confirm the residence time when sink node stays in each position through linear programming. Finally, the paper makes simulation al analysis aiming at above-mentioned routing protocols . The result shows that the inside node energy consumption is comparatively balanced and efficient, and the network lifetime is prolonged effectively.

Density functional theory (DFT) calculations are performed to investigate the structures of phenyl silsesquioxanes Si2nO3nPh2n (n=1-5). Our study focuses on the structures, stabilities, and electronic properties of the phenyl silsesquioxanes. The large HOMO–LUMO gaps, which range from 5.14 to 6.30 eV, imply optimal electronic structures for these molecules. The energy differences between the possible conformers of same size phenyl silsesquioxanes are small.

The main objective of this research is to investigate the effects of astragaloside IV, calycosin separately glucoside, formononetin on oxidative stress in Chang Liver cells induced by H2O2. In the experiments, Chang Liver cells (a kind of normal human hepatocytes) were used as the research object, bifendate which has a clear hepatoprotective effect was used as the positive control drug, then the oxidative damage model of Chang Liver cells were established by H2O2. Cells were divided into six groups: blank control group, oxidative stress group, astragaloside IV group, calycosin separately glucoside group, formononetin group and positive control group. Then endogenous antioxidant system related indexes were detected by micro plate and colorimetric method; intracellular reactive oxygen species (ROS) were detected by DCFH-DA fluorescent probe; and the expressions of CYP2E1 were evaluated by liver microsomes, mRNA, and protein, respectively with spectrophotometry, Real-time PCR method, and Western blot technique. Results showed that H2O2 decreased antioxidant activity, and increased ROS level and expression of CYP2E1. The above oxidative stress status had been changed with protections of the three components of Astragalus membranaceus (compared with oxidative stress group, P0.05, P0.01), which taken as a whole had equivalent effects as the drug of positive control group( bifendate). Taken together, three Astragalus membranaceus ingredients all had significant or extremely significant inhibiting effects on oxidative damaged Chang Liver cells which were induced by H2O2, and the oxidative damage of Chang Liver cells had been relieved.

To study the protective effect of astragaloside IV on oxidative damages of Chang Liver cells induced by ethanol and H2O2.The alcoholic and nonalcoholic oxidative damage models were established on Chang Liver cells with ethanol and H2O2, respectively. The cells viabilities were detected by MTT assay, transaminase activity and antioxidant ability were detected by micro plate and colorimetric method, reactive oxide species (ROS) was detected by DCFH-DA fluorescent probe and cell cycle was detected by flow cytometry. DNA ladder method was used to detect apoptosis.Both kinds of oxidative damage could decrease the viability and antioxidant enzyme activity of Chang Liver cells, and increase the transaminase activity and MDA content of extracellular fluid. The protective effects of astragaloside IV against those two kinds of oxidative damages were significant or extremely significant. Meanwhile, ethanol could decline the level of ROS significantly in the damaged cells, while H2O2 could increase it significantly. And the effect of astragaloside IV was to make ROS return to the normal level. Retardation of cell cycle progression of Chang Liver cells in G0/G1 induced by ethanol or H2O2 was relieved, and apoptosis was also inhibited.Astragaloside IV had protective effect on oxidative damages of Chang Liver cells induced by ethanol and H2O2.

Temperature-sensitive mutant 2-20/32 of Mycobacterium smegmatis mc(2)155 was isolated and genetically complemented with a Mycobacterium tuberculosis H37Rv DNA fragment that contained a single open reading frame. This open reading frame is designated Rv3265c in the M. tuberculosis H37Rv genome. Rv3265c shows homology to the Escherichia coli gene wbbL, which encodes a dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase. In E. coli this enzyme is involved in O-antigen synthesis, but in mycobacteria it is required for the rhamnosyl-containing linker unit responsible for the attachment of the cell wall polymer mycolyl-arabinogalactan to the peptidoglycan. The M. tuberculosis wbbL homologue, encoded by Rv3265c, was shown to be capable of restoring an E. coli K12 strain containing an insertionally inactivated wbbL to O-antigen positive. Likewise, the E. coli wbbL gene allowed 2-20/32 to grow at higher non-permissive temperatures. The rhamnosyltransferase activity of M. tuberculosis WbbL was demonstrated in 2-20/32 as was the loss of this transferase activity in 2-20/32 at elevated temperatures. The wbbL of the temperature-sensitive mutant contained a single-base change that converted what was a proline in mc(2)155 to a serine residue. Exposure of 2-20/32 to higher non-permissive temperatures resulted in bacteria that could not be recovered at the lower permissive temperatures.

This paper studies the method of making production plan in mass customization. It illuminates that all current methods of making production plan are not suitable for mass customization by analysing the lacks of these methods, and sets up the new method of making production plan in mass customization. The new method can make out the optimize production plan by the math model which is suitable for the case in mass customization. In the models,it is not only considering the production order information from the virtual zoology modularization enterprise information integration flat and rough ability balance, but also considering the weightiness in management and order, the length of the delivery date, and the cost of customization product, the target cost of customization product and the standard product cost. Finally, it makes out a production plan with its models by simulating the case of a mass customization manufactory. (5 pages)