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Abstract Extensive research has probed the impacts of personality traits on student satisfaction, academic anxiety, and performance, with particular attention paid to their implications during the COVID-19 pandemic. Notably, a conspicuous gap is discernible in the existing literature concerning investigations that scrutinize the influence of personality on group learning among different COVID-19 lockdown phases (e.g., pre, during, and post-lockdowns). This study endeavors to fill this gap by undertaking an examination of the fluctuations in group performance within data science curricula. This inquiry considers student cohorts comprising diverse personality traits across distinct phases of lockdowns. Through the investigation of 84 student groups, our research has unearthed intriguing correlations and patterns linking group performance with four influential factors, including personality traits, the lockdown phases (i.e., pre, during and post-lockdowns), the joint factor of lockdowns and personality traits, and the implemented COVID-19 policies (e.g., mask-wearing and social distancing) during the early-stage of back-to-schools. The interesting findings revealed from our studies have the potential to propel the advancement of our comprehension within the field of educational psychology and may serve as catalysts for heightened scrutiny of student personality traits, thereby motivating educators to refine their pedagogical approaches in collaborative learning experiences.

Abstract Lighting up the magma plumbing system beneath a large igneous province (LIP) is challenging because the complex magma migration paths are often covered by flood basalts and sediments. Here, we present a three-dimensional seismic image of the Permian Emeishan LIP in Southwest China, constructed by joint inversion of Rayleigh wave dispersions and receiver functions. The results outline a cylindrical, high-velocity anomaly extending to ~135 km depth below the inner zone of this continental LIP. The geometry and magnitude of the high-velocity anomaly suggest that it represents culminated crystallized materials of primary magmas, thereby mirroring a magma plumbing system fossilized in the lithosphere. Furthermore, our geodynamic models illustrate that the nearly vertical plumbing system was controlled by slow plate motion during the magma emplacement. The plume head beneath a nearly static plate has higher thermal buoyancy and thus promotes more intensive magma emplacement. This phenomenon may apply to other LIPs throughout Earth’s history

Abstract Purpose The research endeavors to explore the implications of CD47 in cancer immunotherapy effectiveness. Specifically, there is a gap in comprehending the influence of CD47 on the tumor immune microenvironment, particularly in relation to CD8 + T cells. Our study aims to elucidate the prognostic and immunological relevance of CD47 to enhance insights into its prospective utilities in immunotherapeutic interventions. Methods Differential gene expression analysis, prognosis assessment, immunological infiltration evaluation, pathway enrichment analysis, and correlation investigation were performed utilizing a combination of R packages, computational algorithms, diverse datasets, and patient cohorts. Validation of the concept was achieved through the utilization of single-cell sequencing technology. Results CD47 demonstrated ubiquitous expression across various cancer types and was notably associated with unfavorable prognostic outcomes in pan-cancer assessments. Immunological investigations unveiled a robust correlation between CD47 expression and T-cell infiltration rather than T-cell exclusion across multiple cancer types. Specifically, the CD47-high group exhibited a poorer prognosis for the cytotoxic CD8 + T cell Top group compared to the CD47-low group, suggesting a potential impairment of CD8 + T cell functionality by CD47. The exploration of mechanism identified enrichment of CD47-associated differentially expressed genes in the CD8 + T cell exhausted pathway in multiple cancer contexts. Further analyses focusing on the CD8 TCR Downstream Pathway and gene correlation patterns underscored the significant involvement of TNFRSF9 in mediating these effects. Conclusion A robust association exists between CD47 and the exhaustion of CD8 + T cells, potentially enabling immune evasion by cancer cells and thereby contributing to adverse prognostic outcomes. Consequently, genes such as CD47 and those linked to T-cell exhaustion, notably TNFRSF9, present as promising dual antigenic targets, providing critical insights into the field of immunotherapy.

Abstract Background Teeth identification has a pivotal role in the dental curriculum and provides one of the important foundations of clinical practice. Accurately identifying teeth is a vital aspect of dental education and clinical practice, but can be challenging due to the anatomical similarities between categories. In this study, we aim to explore the possibility of using a deep learning model to classify isolated tooth by a set of photographs. Methods A collection of 5,100 photographs from 850 isolated human tooth specimens were assembled to serve as the dataset for this study. Each tooth was carefully labeled during the data collection phase through direct observation. We developed a deep learning model that incorporates the state-of-the-art feature extractor and attention mechanism to classify each tooth based on a set of 6 photographs captured from multiple angles. To increase the validity of model evaluation, a voting-based strategy was applied to refine the test set to generate a more reliable label, and the model was evaluated under different types of classification granularities. Results This deep learning model achieved top-3 accuracies of over 90% in all classification types, with an average AUC of 0.95. The Cohen’s Kappa demonstrated good agreement between model prediction and the test set. Conclusions This deep learning model can achieve performance comparable to that of human experts and has the potential to become a valuable tool for dental education and various applications in accurately identifying isolated tooth.

Abstract Background Melanoma is the most lethal skin cancer characterized by its high metastatic potential. In the past decade, targeted and immunotherapy have brought revolutionary survival benefits to patients with advanced and metastatic melanoma, but these treatment responses are also heterogeneous and/or do not achieve durable responses. Therefore, novel therapeutic strategies for improving outcomes remain an unmet clinical need. The aim of this study was to evaluate the therapeutic potential and underlying molecular mechanisms of RC48, a novel HER2-target antibody drug conjugate, either alone or in combination with dabrafenib, a V600-mutant BRAF inhibitor, for the treatment of advanced BRAF-mutant cutaneous melanoma. Methods We evaluated the therapeutic efficacy of RC48, alone or in combination with dabrafenib, in BRAF-mutant cutaneous melanoma cell lines and cell-derived xenograft (CDX) models. We also conducted signaling pathways analysis and global mRNA sequencing to explore mechanisms underlying the synergistic effect of the combination therapy. Results Our results revealed the expression of membrane-localized HER2 in melanoma cells. RC48 effectively targeted and inhibited the growth of HER2-positive human melanoma cell lines and corresponding CDX models. When used RC48 and dabrafenib synergically induced tumor regression together in human BRAF-mutant melanoma cell lines and CDX models. Mechanically, our results demonstrated that the combination therapy induced apoptosis and cell cycle arrest while suppressing cell motility in vitro. Furthermore, global RNA sequencing analysis demonstrated that the combination treatment led to the downregulation of several key signaling pathways, including the PI3K-AKT pathway, MAPK pathway, AMPK pathway, and FOXO pathway. Conclusion These findings establish a preclinical foundation for the combined use of an anti-HER2 drug conjugate and a BRAF inhibitor in the treatment of BRAF-mutant cutaneous melanoma.

The occurrence of a sharp turn along the upper course of the Yangtze River is referred to as the “Great Bend” and represents a large-scale drainage reorganization in response to the surface rise of the Tibetan Plateau. However, the timing and mechanism of the formation of the Great Bend remain disputed. In this paper, we report new (U–Th)/He and apatite fission track thermochronological data from the deep river valley in the Great Bend area of the southeastern margin of the Tibetan Plateau. Compared with the adjacent Jianchuan Basin, two phases of younger rapid cooling for the Great Bend area are identified based on thermal-history modeling, namely, Miocene (ca. 17 to 11–8 Ma) and Quaternary, with the former phase being contemporaneous with the formation of the anticline in the Tiger Leaping Gorge. Progressive increases in the normalized channel steepness (ksn) and the degree of river-valley incision with increasing distance downstream for tributaries of the Yangtze River in the Tiger Leaping Gorge indicate that river rerouting and formation of the Great Bend occurred during the Miocene. Samples located at the bottom of the Tiger Leaping Gorge also reveal a phase of rapid cooling since ca. 1.9 Ma, with an exhumation rate of 1.5 ± 0.2 mm/year. We hypothesize that enhanced Quaternary exhumation in the southeastern margin of the Tibetan Plateau occurred mainly within the narrow region between the Sichuan Basin and the Eastern Himalayan Syntaxis, corresponding to an episode of widespread extensional deformation superimposed above middle- to upper-crustal flexure in this region.

Despite much research in the field of island biogeography, mechanisms regulating insular diversity remain elusive. Here, we aim to explore mechanisms underlying plant species-area relationships in two tropical archipelagoes in the South China Sea. We found positive plant species-area relationships for both coral and continental archipelagoes. However, our results showed that different mechanisms contributed to similar plant species-area relationships between the two archipelagoes. For coral islands, soil nutrients and spatial distance among communities played major roles in shaping plant community structure and species diversity. By contrast, the direct effect of island area, and to a lesser extent, soil nutrients determined plant species richness on continental islands. Intriguingly, increasing soil nutrients availability (N, P, K) had opposite effects on plant diversity between the two archipelagoes. In summary, the habitat quality effect and dispersal limitation are important for regulating plant diversity on coral islands, whereas the passive sampling effect, and to a lesser extent, the habitat quality effect are important for regulating plant diversity on continental islands. More generally, our findings indicate that island plant species-area relationships are outcomes of the interplay of both niche and neutral processes, but the driving mechanisms behind these relationships depends on the type of islands.

Drought stress impairs crop growth and development. BEL1-like family transcription factors may be involved in plant response to drought stress, but little is known of the molecular mechanism by which these proteins regulate plant response and defense to drought stress. Here we show that the BEL1-like transcription factor GhBLH5-A05 functions in cotton (Gossypium hirsutum) response and defense to drought stress. Expression of GhBLH5-A05 in cotton was induced by drought stress. Overexpression of GhBLH5-A05 in both Arabidopsis and cotton increased drought tolerance, whereas silencing GhBLH5-A05 in cotton resulted in elevated sensitivity to drought stress. GhBLH5-A05 binds to cis elements in the promoters of GhRD20-A09 and GhDREB2C-D05 to activate the expression of these genes. GhBLH5-A05 interacted with the KNOX transcription factor GhKNAT6-A03. Co-expression of GhBLH5-A05 and GhKNAT6-A03 increased the transcription of GhRD20-A09 and GhDREB2C-D05. We conclude that GhBLH5-A05 acts as a regulatory factor with GhKNAT6-A03 functioning in cotton response to drought stress by activating the expression of the drought-responsive genes GhRD20-A09 and GhDREB2C-D05.

Being Earth’s only natural satellite, the moon is crucial for human space exploration and serves as a vital base for additional deep space endeavors. Cislunar space represents an extensive domain for expanding human habitation beyond terrestrial land and oceans. The pursuit, building, and advancement of cislunar space have escalated the need for cislunar navigational capabilities. Currently, GNSS navigation technology has been well-established for Earth and near-Earth regions and is now being extended to encompass the cislunar realm. Concurrently, the progress in small satellite technology presents fresh opportunities for the rapid and effective implementation of small satellite navigation networks. Addressing cislunar navigation needs, this document suggests creating a navigation system in small space using BDS (BeiDou Navigation Satellite System) time-transfer technology on a compact satellite platform in earth-moon libration point orbits. A variety of orbital paths, such as DRO (Distant Retrograde Orbit) and NRHO (Near Rectilinear Halo Orbit), are employed to form a navigational constellation under the framework of a small satellite platform operating in earth-moon libration point orbits; BDS timing is utilized to compensate for limited payload capacity on small satellites as well as limitations in star clock accuracy. This paper presents the design architecture analysis of such a cislunar space small-satellite navigation system employing BDS time-transfer technology while systematically studying various multi-orbital characteristics adopted by this system along with its corresponding navigational features. Furthermore, observability analysis is conducted on these navigational constellations while evaluating their performance through relevant accuracy indicators across several constellation design scenarios. This paper confirms the practicality and initial efficacy of the cislunar space small satellite navigation system, employing BDS for time-transfer, serving as an essential guide for BDS and GNSS in providing navigational services in cislunar space. An innovative idea and approach for navigating lunar space and the southern hemisphere’s moon is suggested.

This paper conducts an in-depth study of a wireless, hierarchical structure-based active balancing system for power batteries, aimed at addressing the rapid advancements in battery technology within the electric vehicle industry. The system is designed to enhance energy density and the reliability of the battery system, developing a balancing system capable of managing cells with significant disparities in characteristics, which is crucial for extending the lifespan of lithium-ion battery packs. The proposed system integrates wireless self-networking technology into the battery management system and adopts a more efficient active balancing approach, replacing traditional passive energy-consuming methods. In its design, inter-group balancing at the upper layer is achieved through a soft-switching LLC resonant converter, while intra-group balancing among individual cells at the lower layer is managed by an active balancing control IC and a bidirectional buck–boost converter. This configuration not only ensures precise control but also significantly enhances the speed and efficiency of balancing, effectively addressing the heat issues caused by energy dissipation. Key technologies involved include lithium-ion batteries, battery management systems, battery balancing systems, LLC resonant converters, and wireless self-networking technology. Tests have shown that this system not only reduces energy consumption but also significantly improves energy transfer efficiency and the overall balance of the battery pack, thereby extending battery life and optimizing vehicle performance, ensuring a safer and more reliable operation of electric vehicle battery systems.

Abstract Whether tectonic strain from the early stage India‐Asia collision has synchronously affected the far‐field margin of the Tibetan Plateau is crucial for understanding plateau deformation and growth processes. However, direct evidence for early far‐field deformation remains scarce. Utilizing illite K‐Ar dating of three fault gouge samples, we established the faulting history of the Leibo fault zone (LFZ) at the southeastern margin of the Tibetan Plateau (SEMTP). Consistent authigenic illite ages of 52 ± 2, 54 ± 12 and 55 ± 6 Ma suggest the reactivated thrust faulting of the LFZ in the Early Cenozoic. Positioned ∼700 km east of the collisional boundary and at the intersection of three blocks with distinct lithospheric rheology in strength/viscosity, this event suggests a quasi‐synchronous far‐field tectonic response in the SEMTP to the India‐Asia collision.

Abstract Introduction Fungal communities are key players in the soil biogeochemical processes of forest ecosystems. Although it has been illustrated that soil fungi are susceptible to environmental changes, little is known about the interactive effects of nitrogen (N) and phosphorus (P) enrichment on the soil fungal functional guild. Materials and Methods Here, a 5‐year N and P addition manipulation experiment was conducted in two growing stages (i.e., tree ages 80 years) of a subtropical forest, in which soil fungal diversity and functional guilds were investigated using a multiple‐time sampling strategy. Results Fungal species richness and Shannon indices were significantly different across sampling times. Fungal community composition was significantly affected by both N and P addition but not by forest age. Old forest had a higher fungal network complexity than young forest, and fertilisation decreased soil fungal network complexity and generated looser and more random networks in comparison with the control. The community composition of symbiotrophic and saprotrophic fungi was significantly impacted by N and P addition and correlated with soil available P and total P contents and soil C:P and N:P. Conclusions Our findings highlight that soil P availability has a strong effect on soil fungal communities and their functional guild composition in the subtropical forest.

With the rapid development of hydrogen production by water electrolysis, the coupling between the electricity-hydrogen system has become closer, providing an effective way to consume surplus new energy generation. As a form of centralized management of distributed energy resources, virtual power plants can aggregate the integrated energy production and consumption segments in a certain region and participate in electricity market transactions as a single entity to enhance overall revenue. Based on this, this paper proposes an optimal scheduling model of an electricity-hydrogen coupling virtual power plant (EHC-VPP) considering hydrogen load response, relying on hydrogen to ammonia as a flexibly adjustable load-side resource in the EHC-VPP to enable the VPP to participate in the day-ahead energy market to maximize benefits. In addition, this paper also considers the impact of the carbon emission penalty to practice the green development concept of energy saving and emission reduction. To validate the economy of the proposed optimization scheduling method in this paper, the optimization scheduling results under three different operation scenarios are compared and analyzed. The results show that considering the hydrogen load response and fully exploiting the flexibility resources of the EHC-VPP can further reduce the system operating cost and improve the overall operating efficiency.

Abstract Background Leptospirosis is a zoonotic disease that afflicts both humans and animals. It progresses from flu-like symptoms to more severe hepatic and renal failure, and may also lead to aseptic meningitis. Individuals with autoimmune diseases (ADs) are potentially more susceptible to Leptospirosis. Thus far, limited data has documented the association between Leptospirosis and autoimmune disorders. Case presentation The patient had a definitive pathological diagnosis of Sjögren’s syndrome (SS). Due to recurrent headaches, the patient sought consultation with a neurologist. Lumbar puncture revealed elevated white blood cells and protein levels in the cerebrospinal fluid, along with decreased glucose. Tuberculous meningitis was suspected. Radiographic imaging exhibited meningeal enhancement, ventricular enlargement, and hydrocephalus. The patient commenced treatment with anti-tuberculosis therapy and corticosteroids. Subsequently, high-throughput sequencing (HTS) of cerebrospinal fluid identified the presence of Leptospira interrogans. The patient was ultimately diagnosed with Leptospiral meningitis, and underwent antimicrobial and immunosuppressive therapy, resulting in stabilization of the condition and gradual symptom recovery. Conclusions The case highlights the challenges in diagnosing and managing leptospirosis-related meningitis in the presence of ADs and emphasizes the importance of utilizing HTS for accurate pathogen detection. The potential correlation between leptospirosis and SS warrants further investigation, as does the need for multidisciplinary involvement in treatment strategies for such complex cases.

Abstract Bacterial gene repertoires reflect adaptive strategies, contribute to ecosystem functioning and are limited by genome size. However, gene functional diversity does not necessarily correlate with taxonomic diversity because average genome size may vary by community. Here, we analyse gene functional diversity (by shotgun metagenomics) and taxonomic diversity (by 16S rRNA gene amplicon sequencing) to investigate soil bacterial communities along a natural pH gradient in 12 tropical, subtropical, and temperate forests. We find that bacterial average genome size and gene functional diversity decrease, whereas taxonomic diversity increases, as soil pH rises from acid to neutral; as a result, bacterial taxonomic and functional diversity are negatively correlated. The gene repertoire of acid-adapted oligotrophs is enriched in functions of signal transduction, cell motility, secretion system, and degradation of complex compounds, while that of neutral pH-adapted copiotrophs is enriched in functions of energy metabolism and membrane transport. Our results indicate that a mismatch between taxonomic and functional diversity can arise when environmental factors (such as pH) select for adaptive strategies that affect genome size distributions.

Abstract High-resolution seismic image is critically important for mining minerals. In this study, we collected seismic data from a local dense seismic array consisting of 154 stations around the Anyuan mining area and its adjacent region of Pingxiang City, Jiangxi Province in South China, and applied the ambient noise tomography (ANT) method to image the shear wave velocity structure in the study area. Shallow crustal velocities at depths less than 3.3 km were determined by direct inversion of Rayleigh wave group velocity dispersion curves at the period range of 0.5–5.0 s. Overall, the S-wave velocity structure has a tight correlation with surface geological and tectonic features in the study area. The shear wave velocity structure in the shallow crust of the Anyuan Mine and its adjacent areas displayed distinct low-velocity anomalies, which can be attributed to the depression of sedimentary structures and coal mining activities in the Pingxiang-Leping region. The zones surrounding the Anyuan fault (AYF) and Wangkeng fault (WKF) zones exhibited low-velocity anomalies from the ground surface to ~ 3.3 km underground. And the low-velocity anomalies at depths less than 1.2 km could be related to the sedimentary environment of coal mine and the coal mining activities, while the low-velocity anomalies at depths below 1.2 km are caused by the presence of fracture medium, oil and gas in the fault zone. The shear wave velocity changes sharply across the AYF, and the characteristics of the velocity change interface indicate that the AYF is inclined toward the northwest, with its extension reaching depths of approximately 3 km underground. Graphical Abstract

Abstract Background To describe the preferred cataract surgery practices among Malaysian ophthalmologists and medical officers in comparison with the recommended practices. Methods An online questionnaire was sent out in April 2021, to Malaysian Ophthalmologists and medical officers who perform cataract surgeries. The questions were focused on the preferred cataract surgery practices of the participants. All data obtained were collected, tabulated and analysed. Results A total of 173 participants responded to the online questionnaire. 55% of the participants were within 31–40 years of age. 56.1% preferred peristaltic pump over venturi system. 91.3% of participants practised povidone iodine instillation to the conjunctival sac. With regards to the main wound incision, more than half of the surgeons (50.3%) preferred fixed superior incision and 72.3% of them preferred 2.75 mm microkeratome blade. Most of the participants (63%) were inclined towards C-Loop clear intraocular lens (IOL) with a single-handed push preloaded system. 78.6% of the surgeons routinely use carbachol in their cataract surgery. Conclusions This survey provides some insight into the current practices among Malaysian ophthalmologists. Most of the practices are in line with international guidelines for preventing postoperative endophthalmitis. This article could help trainees and ophthalmologists benchmark and observe the common cataract surgery practices among their seniors and peers in Malaysia.

The activation of H2O is a key step of the COS hydrolysis, which may be tuned by oxygen vacancy defects in the catalysts. Herein, we have introduced Cu into Co3O4 to regulate the oxygen vacancy defect content of the catalysts. In situ DRIFTS and XPS spectra reveal that COS and H2O are adsorbed and activated by oxygen vacancy. The 10 at% Cu doped Co3O4 sample (10CuCo3O4) exhibits the optimal activity, 100% of COS conversion at 70 °C. The improved oxygen vacancies of CuCo3O4 accelerate the activation of H2O to form active OH. COS binds with hydroxyl to form the intermediate HSCO2−, and then the activated –OH on the oxygen vacancy reacts with HSCO2− to form HCO3−. Meanwhile, the catalyst exhibits high catalytic stability because copper species (Cu+/Cu2+) redox cycle mitigate the sulfation of Co3O4 (Co2+/Co3+). Our work offers a promising approach for the rational design of cobalt-related catalysts in the highly efficient hydrolysis COS process.

BackgroundPrimary cardiac lymphoma is an extremely rare malignant lymphoma, with clinical manifestations related to its location. We reported the diagnosis and treatment of primary cardiac lymphoma in a patient presented with atrioventricular block.Case presentationA 64 year-old man was admitted to our hospital because of symptoms of a tired heart and shortness of breath. The initial electrocardiogram revealed a third-degree atrioventricular block. Computed tomography scan showed an irregularly shaped right heart, irregular clusters, and relatively weakly enhanced areas in the right auricle, atrium, and ventricle. The local boundary between the lesion, pericardium, and left atrium was unclear, and the ventricular septum was irregular and thickened. Multiple irregular gray neoplasms with less smooth surfaces were observed, with a maximum diameter of approximately 7 cm. Pathological findings confirmed a non-germinal center B cell subtype of diffuse large B-cell lymphoma. After surgical resection of the tumor and implantation of a permanent pacemaker, the symptoms of the patient were significantly improved, allowing subsequent chemotherapy.ConclusionSurgical resection and placement of a permanent pacemaker were effective treatments for a patient with primary cardiac lymphoma presented with atrioventricular block.

Abstract The influence of personality traits on educational outcomes has been widely recognized and studied. Research has explored its effects on factors such as student satisfaction, academic anxiety, and dishonesty, particularly during the COVID-19 pandemic. However, there has been a lack of studies comparing the learning behaviors and performance of students with different personality traits during the pre, during, and post-COVID-19 lockdown periods. This study fills this gap by analyzing the differences in academic metrics, such as class grades and assignment submissions, among students with varying personality traits during different lockdown periods. Our research, based on a dataset of 282 graduate students in the USA, identified correlations and patterns between lockdown periods, personality traits, and academic metrics. For example, the class grades and the rate of late submissions were affected by different lockdown periods. Students with lower degree in extraversion and agreeableness made less attempts in assignment submissions. These findings can assist educators in identifying impacted students and developing effective teaching strategies at early stage in future incidents.

Abstract Background Multiple sclerosis is a diffuse chronic demyelinating disease of the central nervous system. It is relatively uncommon in the Asian population and even more so in males. Despite the usual involvement of the brainstem, eight-and-a-half syndrome remains a rare first presentation in multiple sclerosis. Only a few cases have been reported previously, but none involving the Asian population. Eight-and-a-half syndrome, a neuro-ophthalmological condition, is characterized by one-and-a-half syndrome with ipsilateral lower facial nerve palsy, which localizes lesions to the pontine tegmentum. This case report demonstrates the first case of eight-and-a-half syndrome as the first presentation of multiple sclerosis in an Asian male. Case presentation A healthy 23-year-old Asian man presented with sudden onset of diplopia followed by left-sided facial asymmetry for 3 days. Assessment of extraocular movement revealed left conjugate horizontal gaze palsy. On right gaze, there was limited left eye adduction and horizontal nystagmus of the right eye. These findings were consistent with a left-sided one-and-a-half syndrome. Prism cover test revealed left esotropia of 30 prism diopters. Cranial nerve examination showed left lower motor neuron facial nerve palsy, while other neurological examination was normal. Magnetic resonance imaging brain showed multifocal T2 fluid attenuated inversion recovery hyperintense lesions, involving bilateral periventricular, juxtacortical, and infratentorial regions. A focal gadolinium contrast-enhanced lesion with open ring sign on T1 sequence was seen at the left frontal juxtacortical region. Multiple sclerosis was diagnosed on the basis of the clinical and radiological evidence, which fulfilled the 2017 McDonald criteria. Positive oligoclonal bands in cerebrospinal fluid analysis further confirmed our diagnosis. He had a complete resolution of symptoms 1 month after a course of pulsed corticosteroid therapy, and was subsequently placed on maintenance therapy with interferon beta-1a. Conclusion This case illustrates eight-and-a-half syndrome as the first presentation of a diffuse central nervous system pathology. A wide range of differential diagnoses needs to be considered in such a presentation as based on the patient’s demographics and risk factors.

Abstract Star detection is an important part of the spacecraft attitude determination performed by star trackers. The current advanced star detection algorithms can effectively extract star points in complex backgrounds. However, there are still two problems: first, the parameters of the star detection algorithm are not adaptive, which limits its reliability and robustness; second, the high performance and low computational time of algorithms usually cannot be achieved simultaneously. In this work, a rapid star detection algorithm utilizing dyadic wavelet transform (SDDWT) was developed based on the à Trous algorithm. The authors constructed a suitable dyadic wavelet basis and applied the dyadic wavelet transform to the star image. The maximum wavelet coefficients at a certain scale were binarized according to a threshold value to separate stars from the background. The threshold was adaptively determined according to the white Gaussian noise (WGN) power. Finally, a method for separating individual stars was developed. Experiments indicated that the proposed algorithm was able to ensure a low false alarm rate and high detection rate by adaptively threshold value. Moreover, for a star image of 1024×1024, the computational time of the SDDWT algorithm was stable within 0.3 s, which was smaller than other algorithms.

PURPOSE: We aimed to analyze our 4-year experience of intra-arterial chemotherapy (IAC) for retinoblastoma (RB) and to examine the tumor response, globe salvage, mortality, and safety profile of IAC in the Malaysian profile. MATERIALS AND METHODS: This was a retrospective, interventional case series. A total of 22 eyes of 20 patients with RB who underwent IAC using melphalan and topotecan from January 2018 to December 2021 in Hospital Kuala Lumpur were retrospectively reviewed. Tumor response, globe salvage, mortality, and safety profile of IAC were compared based on the International Classification of Retinoblastoma. RESULTS: The mean patient age at IAC was 21.3 months. An overall globe salvage rate of 63.6% was observed: more specifically, 100% for Group A, 75% for Groups B and C, 66.7% for Group D, and 42.9% for Group E. Poor tumor response after IAC was significantly associated with a lesser chance of globe salvage (P = 0.045). The overall rate of good tumor response following IAC was 77.3%. Specifically, rates of good tumor response in each group were 100%, 75%, 75%, 83.3% and 71.4% in group A, B, C, D and E, respectively. The mortality rate was 5%. Complications (per-catheterization) included cerebral infarct (2.2%), oxygen desaturation (2.2%), vomiting (26.1%), periorbital edema (8.8%), ptosis (6.5%), fever, femoral hematoma, and hyperpigmentation over lid (4.4% each). CONCLUSION: Four-year experience showed that IAC is a safe and effective method for RB management. Patients with a poor response after IAC may have a lower chance of globe salvage. Careful patient selection is of utmost importance to achieve the best outcome in a setting of limited health-care resources.

Emerging evidences suggest that miRNAs can be used as theranostic biomarkers for multiple cancers, including glioma. Thus, identification of novel miRNAs for glioma treatment and prognosis becomes necessary and urgent. Here, by analyzing miRNA expression profiles in the glioma and para-cancer tissues by miRNA microarray and verified by RT-PCR, we found that miR-133a was significantly downregulated in the cancerous tissues, and patients with low-expressed miR-133a levels predicted an unfavorable prognosis. The following functional experiments confirmed that overexpression of miR-133a restrained cell proliferation and colony formation abilities, and induced cell cycle arrest to restrain cancer progression in glioma cells. Then, the underlying mechanisms were uncovered, and the peroxisome proliferator-activated receptor γ (PPARγ, PPARG) was verified as the downstream target of miR-133a. Mechanistically, miR-133a negatively regulated PPARG expressions by binding to its 3’ untranslated regions (3’UTR). The following rescuing experiments evidenced that miR-133a overexpression-induced anti-cancer effects in glioma cells were abrogated by upregulating PPARγ. Interestingly, we noticed that the promoter region of miR-133a was hypermethylated, and removal of DNA methylation by 5-Azacytidine (AZA) significantly increased the expression levels of miR-133a in glioma cells. Taken together, we concluded that DNA-methylation-induced miR-133a silence contributed to cancer progression in glioma through upregulating PPARγ, and firstly identified the DNA-methylation-regulated miR-133a/PPARG axis as the novel indicators for glioma treatment and prognosis.

Highlights Cattle dung can be effectively managed through vermicomposting Biochar showed both positive and negative effects on earthworms’ performance 5% biochar addition promoted compost quality by stimulating earthworms’ activity Higher doses of biochar were not suitable for vermicomposting Available N and functional genes drove compost quality and N2O emissions Biochar increased AOB but decreased nirS gene numbers in fresh cast of earthworms

During hypersonic flight in the atmosphere, severe aerothermal effects occur on the optical hood or observation window of an aircraft, keeping the optical window and surface flow field in a high-temperature state, as well as causing a large amount of radiation and strong background noise. These phenomena have serious effects on the observation capability of star sensors. An evaluation model of star sensor observation capability under hypersonic aerothermal conditions was constructed in this study, using a definition of the signal-to-noise ratio considering defocused intense radiation sources. Furthermore, a method of calculating the observable limiting magnitude under aerothermal conditions is given. The model was used in simulation tests involving different flight states and temperature conditions. The results showed that the thermal radiation of the aircraft observation window has the greatest influence, which is more than two orders higher than that of the outer flow near the window; when the window temperature was controlled below 750 K, for a star sensor with an SNR threshold of 3, navigation stars with magnitudes brighter than 5 could be captured in the visible band of 0.35– $0.7~\mu \text{m}$ , and the near-infrared band of 0.9– $1.4~\mu \text{m}$ was unavailable.

Developing a suitable catalyst for the elimination of highly toxic carbonyl sulfide (COS) and hydrogen sulfide (H2S) is of great significance in terms of industrial safety and environmental protection. We demonstrate here the facile synthesis of graphitized 2D micro-meso-macroporous carbons by one-step carbonization of a mixture of urea and glucose at 700–900 °C. The as-synthesized graphitized catalysts, designated as 2D-NHPC-x (x = urea/glucose mass ratio), are endowed with an ultra-high concentration (12.9–20.2 wt%) of stable and versatile nitrogen sites (e.g. pyrrole and pyridine) which are anchored on the surface via stable covalent bonding. As a result, the 2D-NHPC-x are active in catalytic hydrolysis of COS on pyrrolic N to H2S, and the H2S can be subsequently captured on pyridinic N and converted to elemental sulfur at ambient conditions over the same materials. Among the prepared catalysts, 2D-NHPC-x can catalytically hydrolysize 91% of COS to H2S at 30 °C, whereas the conversion ratio over the common catalysts g-C3N4 and Fe2O3 are below 6.0%. Furthermore, these catalysts also exhibit H2S conversion and sulfur selectivity of nearly 100% at 180 °C with long-time durability, which is higher than those of the most reported carbon-based catalysts. In contrast, the H2S capacities of activated carbon, ordered mesoporous carbons (OMC) and N-doped OMC are 3.9, 1.5 and 2.39 mmol g−1, respectively. Both the experimental and theoretical results are disclosed that 2D-NHPC-x are superior to the nitrogen-doped porous materials ever applied in simultaneous catalytic elimination of both COS and H2S.

Supercritical CO2 fracturing is an important development trend to reach the goal of ''dual carbon'' and avoid the problem that hydraulic fracturing is influenced by water resource. In order to clarify the transport characteristics of proppant in the fractures induced by supercritical CO2 fracturing, this paper reconstructs the fracture surface of rock samples after supercritical CO2 fracturing using the laser morphological scanning technology, and establishes a model of proppant carrying and transport of supercritical CO2 in tortuous fractures on the basis of CFD-DEM method. In addition, the transport and placement characteristics of proppant in tortuous fractures are analyzed by comparing with flat fractures, and the effects of proppant density, injection rate of proppant carrying liquid, proppant concentration and other key parameters on proppant transport and distribution in fractures are investigated. And the following research results are obtained. First, compared with those in flat fractures, the flow paths of the proppant carrying supercritical CO2 liquid in tortuous fractures are tortuous and diverse, and the proppant presents stronger fluctuations and jumps laterally and vertically during its transport. Second, the proppant placement in tortuous fractures morphologically presents a wavy or even clustered non-uniform distribution. Third, low-density proppant has a better pass-ability in tortuous fractures, and the high injection rate can reduce the influence of tortuous fracture structure on proppant blocking. Fourth, if the concentration of injected proppant in the tortuous fracture is too low, good fracturing support effect cannot be achieved, and the optimal value under the simulation conditions in this paper is around 3%. In conclusion, the simulation results are of important theoretical and engineering significance to understanding the mechanism of proppant blocking in the pumping process of proppant carrying liquid for supercritical CO2 fracturing and optimizing the field fracturing design.

This study investigated the grouped and individualized load-velocity profile (GLVP vs. ILVP) in Bulgarian split squat using Smith machine and free weight. Seventy five recreational male lifters completed two incremental loading tests of Bulgarian split squat. Mean velocity was measured by a linear-position transducer (GymAware). Linear regression equation was applied to construct the GLVP and ILVP. The agreement of predicted %1RM and measured %1RM was assessed by a combination of intraclass correlation coefficient (ICC), coefficient of variation (CV), standard error of measurement (SEM) and Bland-Altman analysis. Acceptable validity was defined as ICC > 0.75, CV ≤ 10% and p ≥ 0.05 (a paired Wilcoxon signed-rank test). A very high level of inverse load-velocity relationships were demonstrated in Bulgarian split squat (r = − 0.92) with free weights and a Smith machine. ILVP (ICC ≥ 0.98, CV ≤ 8.73%, p ≥ 0.56) was valid enough to predict the %1RM, but GLVP of both limbs revealed large CVs in free weights (CV: 15.4%,15.63%) and a Smith machine (CV: 11.24%, 12.25%). Cross-validation between the actual %1RM and predicted %1RM using free weights and a Smith machine ILVP was not acceptable (p ≤ 0.03, CV ≥ 14.07%). A very high level of inverse relationship were observed between %1RM and MV in Bulgarian split squat using free weights and a Smith machine, indicating individualized load velocity properties, and the ILVP showed high between-devices variability in both scenarios. Using velocity as a measure of loading intensity in Bulgarian split squat needs to consider the individualized load velocity properties, and difference between free weights and a Smith machine.

The performance of ethylene/1-octene copolymer primarily depends on the microstructure of the polymer chain. This study employed a new method to control the inter-distribution of hexyl chain branches directly on the backbone of the ethylene/1-octene copolymer. Three ethylene/1-octene copolymers with different inter-distributions of hexyl chain branches were synthesized using [Me2Si(C5Me4) (NtBu)] TiCl2 (Ti–CGC) by different feeding sequences in the semi-continuous polymerization reaction system. The three copolymers were named according to the feeding sequence of the materials: ethylene/1-octene/Ti–CGC (EOC), 1-octene/Ti–CGC/ethylene (OCE), and ethylene/Ti–CGC/1-octene (ECO), respectively. The structure and properties of the copolymers were characterized using HT-GPC, 13C-NMR, DSC, WAXD, DMA, MI, and Uniaxial Tension Test. The results showed that the feeding sequence greatly affected the comonomer distribution of the molecular chains, molecular weight, molecular weight distribution, and chemical composition of the copolymers, consequently influencing their thermal performance and mechanical properties. Thus, it is probable that one could obtain an ethylene/1-octene copolymer with designed properties by controlling the feeding sequence during the ethylene/1-octene semi-continuous copolymerization process.

The Large Magellanic Cloud (LMC) is home to many H ii regions, which may lead to significant outflows. We examine the LMC’s multiphase gas ( T ∼10 ^4-5 K) in H i , S ii , Si iv , and C iv using 110 stellar sight lines from the Hubble Space Telescope’s Ultraviolet Legacy Library of Young Stars as Essential Standards program. We develop a continuum fitting algorithm based on the concept of Gaussian process regression and identify reliable LMC interstellar absorption over v _helio = 175–375 km s ^−1 . Our analyses show disk-wide ionized outflows in Si iv and C iv across the LMC with bulk velocities of ∣ v _out, bulk ∣ ∼ 20–60 km s ^−1 , which indicates that most of the outflowing mass is gravitationally bound. The outflows’ column densities correlate with the LMC’s star formation rate surface densities (Σ _SFR ), and the outflows with higher Σ _SFR tend to be more ionized. Considering outflows from both sides of the LMC as traced by C iv , we conservatively estimate a total outflow rate of ${\dot{M}}_{\mathrm{out}}\gtrsim 0.03\,{M}_{\odot }{\mathrm{yr}}^{-1}$ and a mass-loading factor of η ≳ 0.15. We compare the LMC’s outflows with those detected in starburst galaxies and simulation predictions, and find a universal scaling relation of $| {v}_{\mathrm{out},\ \mathrm{bulk}}| \propto {{\rm{\Sigma }}}_{\mathrm{SFR}}^{0.23}$ over a wide range of star-forming conditions (Σ _SFR ∼ 10 ^−4.5 –10 ^2 M _⊙ yr ^−1 kpc ^−2 ). Lastly, we find that the outflows are corotating with the LMC’s young stellar disk and the velocity field does not seem to be significantly impacted by external forces; we thus speculate on the existence of a bow shock leading the LMC, which may have shielded the outflows from ram pressure as the LMC orbits the Milky Way.

We present a sample of 305 QSO candidates having ∣ b ∣ < 30°, the majority with GALEX magnitudes near-UV < 18.75. To generate this sample, we apply UV–IR color selection criteria to photometric data from the Ultraviolet Galactic Plane Survey as part of GALEX-CAUSE, the Million Quasars Catalog, Gaia DR2, and Pan-STARRS DR1. 165 of these 305 candidate UV-bright active galactic nuclei (AGN; 54%) have published spectroscopic redshifts from 45 different surveys, confirming them as AGN. We further obtained low-dispersion, optical, long-slit spectra with the Apache Point Observatory 3.5 m, MDM 2.4 m, and MDM 1.3 m telescopes for 84 of the candidates, and confirm 86% ( N = 72) as AGN, generally with z < 0.6. Of these 72 confirmed AGN, 25 are newly discovered low-latitude QSOs without any previous spectroscopy. These sources fill a gap in the Galactic latitude coverage of the available samples of known UV-bright QSO background probes. Along with a description of the confirmed QSO properties, we provide the fully reduced, flux- and wavelength-calibrated spectra of 72 low-latitude QSOs through the Mikulski Archive for Space Telescopes. Future Hubble Space Telescope/Cosmic Origins Spectrograph spectroscopy of these low-Galactic-latitude QSOs has the potential to transform our view of the Milky Way and Local Group circumgalactic medium.

We present a new technique to identify associations of H i emission in the Magellanic Stream (MS) and ultraviolet (UV) absorbers from 92 QSO sight lines near the MS. We quantify the level of associations of individual H i elements to the main H i body of the Stream using Wasserstein distance-based models, and derive characteristic spatial and kinematic distances of the H i emission in the MS. With the emission-based model, we further develop a comparison metric, which identifies the dominant associations of individual UV absorbers with respect to the MS and nearby galaxies. For ionized gas associated with the MS probed by C ii , C iv , Si ii , Si iii , Si iv , we find that the ion column densities are generally ∼0.5 dex higher than those that are not associated, and that the gas is more ionized toward the tail of the MS as indicated by the spatial trend of the C ii /C iv ratios. For nearby galaxies, we identify potential new absorbers associated with the circumgalactic medium of M33 and NGC 300, and affirm the associations of absorbers with IC 1613 and the Wolf–Lundmark–Mellote galaxy. For M31, we find the previously identified gradient in column densities as a function of the impact parameter, and that absorbers with higher column densities beyond M31's virial radius are more likely to be associated with the MS. Our analysis of absorbers associated with the Magellanic Clouds reveals the presence of continuous and blended diffuse ionized gas between the Stream and the Clouds. Our technique can be applied to future applications of identifying associations within physically complex gaseous structures.

The cycling of metals between interstellar gas and dust is a critical aspect of the baryon cycle of galaxies, yet our understanding of this process is limited. This study focuses on understanding dust depletion effects in the low-metallicity regime (

All-natural rubber is harvested from rubber trees (Hevea brasiliensis Muell. Arg.) by traditional tapping knives, so rubber tapping still heavily relies on labor. Therefore, this study explored a novel, hand-held mechanical rubber tapping machine for rubber tree harvesting. In this study, a mechanical tapping cutter with a vertical blade and adjustable guide was first described. The response surface method was applied to evaluate factors affecting the tapping effect. The experimental values were in close agreement with the predicted value. Machine-tapped latex was comparable in quality to hand-tapped latex. Based on the single-factor results, the response surface method (RSM) and the center combined rotation design (CCRD) optimization method were adopted to explore the influence of three factors influencing vertical blade height (A), cutting force (B), and spiral angle (C) on the tapping effect. Regarding the cutting rate of the old rubber line (Y1), cutting time (Y2), latex flow rate (Y3), and average cutting current (Y4) as evaluation indexes of the tapping effect, an optimization scheme was determined. The quadratic model fits for all the responses. The test results showed that the main factors affecting Y1, Y2, Y3, and Y4 were A and B, B, A and C, and B, respectively. Under optimal conditions, the influencing factors of A, B, and C were 10.24 mm, 51.67 N, and 24.77°, respectively, when the evaluation index values of Y1, Y2, Y3, and Y4 were 98%, 8.65 mL/5 min, 9.00 s, and 1.16 A. The range of the relative error between the experimental and predicted results was from −11.11% to 11.11%. According to the optimized treatment scheme, a comparison test was designed between mechanical and manual rubber tapping tools. To verify the availability and effect of the mechanical tapping method preliminarily, the important rubber tapping evaluation indexes included bark thickness, bark excision, latex flow time, cutting time, ash content, and cutting depth, which were selected to serve as a comparison test. There was no significant difference between hand and mechanical methods, except ash content (p < 0.05) and cutting time (p < 0.01). The mechanical tapping machine proposed in this study is meaningful to improve cutting efficiency, practicality, and operability. Furthermore, it provides crucial theoretical references for the development of intelligent tapping machines.

The cultivated land requisition-compensation balance (CLRB) system in China has been designed to defend cultivated land resources and grain production functions. Nevertheless, since the addition of a new policy, namely the linkage of increase and decrease (LID) of urban and rural construction land in 2008, a mass of cultivated land has been returning to mountains, sometimes resulting in abandonment. The county of Wannian was investigated from the microcosmic perspective, and we attempted to analyze the causes and risks employing the boosted regression trees (BRT) model and the grain productive capacity assessment model. The results indicate that (1) The compensatory cultivated land (CCL) has shifted uphill, with considerable fragmentation, from 2010 to 2020, and the abandonment rate reached 14.77%. (2) The factors of site condition, including elevation, patch area, and continuity of cultivated land, as well as a series of combinations, can explain the causes of abandonment. (3) The abandonment of these cultivated land areas eventually resulted in the risk of 297.48 t grain production capacity loss. The reason for the return of cultivated land to the mountains and its subsequent abandonment is the lack of consideration for the coupling relationship among site condition, use state, and function requirement, resulting in a spatial mismatch. Based on the findings, we propose a solution of the natural resources requisition-compensation balance (NRRB). To make up for the loss and reduce the risk, a spatial replacement was taken between the abandoned CCL in uphill and cultivable and available forest land (CAFL) in submontane areas CCL, optimizing the spatial pattern of land use toward Von Thunen’s agricultural circle.

Abstract Background Familial Exudative Vitreoretinopathy (FEVR) is a hereditary disorder characterized by peripheral avascular retina with neovascularization. Although FEVR has been thoroughly described in multiple literature publications from different countries, there are currently limited articles describing the phenotypes of FEVR among South-East Asian Descendent. This paper describes the clinical phenotype of the FZD4 gene with c.1501_1502 deletion in a 4-generation case series of a South East Asian family. Methods We reviewed a 4-generation case series of a South-East Asian descendent family consisting of 27 family members with 10 members diagnosed with FEVR. We observed the clinical phenotype of these series of patients, including some of the family members who underwent whole-exome sequencing, PCR amplification and DNA sequencing techniques to identify the mutated gene. Results Frameshift mutation (c.1501_1502del) were found in FZD4 gene in this series of patients with the age ranging from 1 month old to 69 years old. There was a 100% (4/4) of our paediatric patients being diagnosed within 21 days of life. It was also found that 75% of patients (6/8) less than 40 years old exhibited disease asymmetry of 2 stages or more and 80% (8/10) had a history of vitreoretinal surgery or diode laser photocoagulation, with a further 50% of the adult patients identified as legally blind; the mean age of blindness was 18-years-old. Conclusions Phenotypic manifestation of FZD4 gene with c.1501_1502del mutation can be identified within the neonatal period. They have relatively greater clinical asymmetry of 2 stages or more compared to other mutations. Without treatment, most of them will have bilateral severe visual impairment around the adolescent age group.

Abstract Background We report a rare case of Godtfredsen syndrome caused by clival chondrosarcoma and perform a review of literatures. This article also explains the clinico-anatomical correlation of this rare neurological syndrome. Case presentation A 22-year-old gentleman presented with binocular diplopia. Clinical examination revealed an isolated right abducent nerve and right hypoglossal nerve palsy, with other cranial nerves intact. Neuroimaging revealed a right clival mass. Supraorbital craniotomy and tumour debulking were done in the same year. Histopathological examination showed low-grade chondrosarcoma. After 5-years of default, he came back with the tumour enlarged. He underwent a right orbitozygomatic craniotomy and tumour excision with 33 cycles of radiotherapy. Despite two surgeries and radiotherapy, the abducent nerve and hypoglossal nerve did not improve throughout 6 years of follow-up. Cranial nerve VI palsy is not always a false localizing sign, in Godtfredsen syndrome it serves as a localizing sign. Conclusion To the best of our knowledge, this is the first case report of Godtfredsen Syndrome secondary to clival chondrosarcoma. Cranial nerve VI and XII palsy with no involvement of other cranial nerves, most likely the pathology is located at the clivus.

We conducted a short-term laboratory soil warming incubation experiment, sampling both warmed and un-warmed soils from a subtropical plantation in southeastern China, incubating them at 20 °C, 30 °C, and 40 °C. Our aim was to study the SOC mineralization response to increasing temperatures. Our findings revealed that the temperature sensitivity (Q10) of SOC mineralization to short-term experimental warming varied between the warmed soil and the un-warmed soil. The Q10 of the un-warmed soil escalated with the temperature treatment (20–30 °C: 1.31, 30–40 °C: 1.63). Conversely, the Q10 of the warmed soil decreased (20–30 °C: 1.57, 30–40 °C: 1.41). Increasing temperature treatments decreased soil substrate availability (dissolved organic C) in both un-warmed and warmed soil. The C-degrading enzyme in un-warmed soil and warmed soil had different trends at different temperatures. In addition, warming decreased soil microbial biomass, resulting in a decrease in the total amount of phospholipid fatty acids (PLFAs) and a decrease in the abundance of fungi and Gram-negative bacteria (GN) in both un-warmed and warmed soil. The ratio of fungal to bacterial biomass (F:B) in un-warming soil was significantly higher than that in warmed soil. A drop in the microbial quotient (qMBC) coupled with a rise in the metabolic quotient (qCO2) indicated that warming amplified microbial respiration over microbial growth. The differential Q10 of SOC mineralization in un-warmed and warmed soil, in response to temperature across varying soil, can primarily be attributed to shifts in soil dissolved organic C (DOC), alterations in C-degrading enzyme activities, and modifications in microbial communities (F:B).

Programmable photonic circuits performing universal linear-optical transformations underpin vital functions in photonic quantum information processing, quantum-enhanced sensor networks, machine learning, and many other intriguing applications. Recent advances in photonic integrated circuits facilitate monolithic integration of externally controlled Mach-Zehnder interferometers (MZIs) which can implement arbitrary unitary transformation on a large number of input/output modes. In this work, we demonstrate a 4×4 programmable linear photonic circuit on lithium niobate on an insulator platform employing fast, power-efficient, and low-loss electro-optical phase shifters, showing enormous advantages in terms of configuration rate and power consumption. Our device composed of cascaded MZIs possesses a total on-chip power dissipation of only 1.5 mW when operating at 100 MHz modulation rate. Our MZIs exhibit high bandwidth of 22.5 GHz, fast switching with 160-ps rise time and 120-ps fall time, low insertion loss of 0.15 dB, and on-chip extinction ratio of −34 dB for both cross and bar routes.

PURPOSE: This study aims to objectively measure the effect of slow tempo music on various markers for anxiety. MATERIALS AND METHODS: This is a repeated measure randomized control trial of patients with age-related cataracts undergoing cataract extraction through phacoemulsification under local anesthesia. Patients were randomized into two groups: music group where a standardized piano music was played and control group. Salivary alpha-amylase (sAA) levels were measured at the beginning and at the end of surgery. Blood pressure and heart rate were also measured at 5 min before surgery and at four other standardized points during and after surgery. Visual Analog Scale for anxiety was also gauged: preoperatively, perioperatively, and 15 min postoperatively. RESULTS: Ninety-two patients were randomized equally to the music group and control group. Paired sample t-test showed a reduction in the level of sAA during surgery in the music group (P = 0.019). The odds ratio for a drop in sAA with music was 4.407 (P = 0.001). Lower systolic blood pressure was observed in the music group: at delivery of local anesthesia (P = 0.047), at first incision (P = 0.023), and during sculpting (P = 0.15). Similarly, diastolic blood pressure was lower at first incision (P = 0.019) in the music group. The visual analog scale for anxiety during surgery was found to be lower in the music group (P = 0.046). CONCLUSION: A slow tempo music during cataract surgery was shown to significantly reduce several indicators for anxiety at various points during cataract surgery.

Recommender systems have been developed to assist decision making by recommending a list of items to the end users. The multi-criteria recommender system (MCRS) is a special type of recommender systems, where user preferences on multiple criteria can be taken into account in recommendation models. Traditional algorithms for MCRS usually predict user ratings on these criteria, and finally estimate the overall rating by different aggregation functions. In this paper, we propose a novel multi-criteria recommendation framework, Multi-Criteria Ranking, where we can directly infer a ranking score for an item candidate from the predicted ratings on multiple criteria. The proposed framework is general enough and most of the existing algorithms in MCRS can be easily integrated with our framework. Our experimental results can demonstrate the effectiveness of the proposed framework by evaluating top- $N$ recommendations over multiple real-world data sets. We believe that multi-criteria ranking opens the door to develop more effective and promising multi-criteria recommendation models.

Bone marrow mesenchymal stem cells (BMSCs) are widely used for therapeutic applications in tissue engineering and regenerative medicine. Nevertheless, the function of BMSCs is adversely affected by senescence. Thus, understanding the molecular mechanisms that contribute to BMSC senescence is critical for the development of BMSC-based tissue engineering and regenerative medicine. In this study, senescent BMSCs were characterized with >80 % of BMSCs stained positive for SA-β-gal, increased expressions of senescence-related genes (p16INK4a and p21Waf1). These senescent characters were accompanied by elevated autophagic activity, up-regulation of interleukin 6 (IL-6), IL-8, and FoxO3a. Autophagic activity inhibition alleviated the senescent state with reduced levels of IL-6 and IL-8 during BMSC senescence. The enhanced autophagic activity upregulated the levels of IL-6 and IL-8 which is associated with up-regulation of FoxO3a, and knockdown of FoxO3a reduced IL-6 and IL-8 expression in senescent BMSCs. Therefore, this study indicated the pivotal role of autophagic activity in the expressions of IL-6 and IL-8 during BMSC senescence, which is regulated by FoxO3a.

The interaction between the supersonic motion of the Large Magellanic Cloud (LMC) and the circumgalactic medium (CGM) is expected to result in a bow shock that leads the LMC’s gaseous disk. In this letter, we use hydrodynamic simulations of the LMC’s recent infall to predict the extent of this shock and its effect on the Milky Way’s (MW) CGM. The simulations clearly predict the existence of an asymmetric shock with a present-day standoff radius of ∼6.7 kpc and a transverse diameter of ∼30 kpc. Over the past 500 Myr, ∼8% of the MW’s CGM in the southern hemisphere should have interacted with the shock front. This interaction may have had the effect of smoothing over inhomogeneities and increasing mixing in the MW CGM. We find observational evidence of the existence of the bow shock in recent H α maps of the LMC, providing a potential explanation for the envelope of ionized gas surrounding the LMC. Furthermore, the interaction of the bow shock with the MW CGM may also explain the observations of ionized gas surrounding the Magellanic Stream. Using recent orbital histories of MW satellites, we find that many satellites have likely interacted with the LMC shock. Additionally, the dwarf galaxy Ret2 is currently sitting inside the shock, which may impact the interpretation of the reported gamma-ray excess in Ret2. This work highlights how bow shocks associated with infalling satellites are an underexplored yet potentially very important dynamical mixing process in the circumgalactic and intracluster media.

Summary: The function of the cancer-associated lncRNA Malat1 during aging is as-of-yet uncharacterized. Here, we show that Malat1 interacts with Nucleophosmin (NPM) in young mouse brain, and with Lamin A/C, hnRNP C, and KAP1 with age. RNA-seq and RT-qPCR reveal a persistent expression of Malat1_2 (the 3’isoform of Malat1) in Malat1Δ1 (5’-1.5 kb deletion) mouse retinas and brains at 1/4th level of the full-length Malat1, while Malat1_1 (the 5’isoform) in Malat1Δ2 (deletion of 3’-conserved 5.7 kb) at a much lower level, suggesting an internal promoter driving the 3’ isoform. The 1774 and 496 differentially expressed genes in Malat1Δ2 and Malat1Δ1 brains, respectively, suggest the 3’ isoform regulates gene expression in trans and the 5’ isoform in cis. Consistently, Malat1Δ2 mice show increased age-dependent retinal oxidative stress and corneal opacity, while Malat1Δ1 mice show no obvious phenotype. Collectively, this study reveals a physiological function of the lncRNA Malat1 3’-isoform during the aging process.

Abstract Background Currently, the prognosis of non‐small‐cell lung cancer (NSCLC) patients remains dismal due to recurrence and metastasis. The purpose of our study was to explore the role of circular RNA_0016760 (circ_0016760) in NSCLC progression and its associated mechanism. Methods Quantitative real‐time polymerase chain reaction (qRT‐PCR) was implemented to measure the expression of circ_0016760, microRNA‐646 (miR‐646) and AK strain thymoma serine/threonine kinase 3 (AKT3). The protein level of AKT3 was examined by Western blot assay. Cell Counting Kit 8 assay, transwell assays, and flow cytometry were conducted to analyze cell proliferation, metastasis, and apoptosis. Dual‐luciferase reporter assay was used to confirm the interactions that were predicted by bioinformatics software (Circular RNA Interactome and TargetScan). A xenograft tumor model was built to investigate the role of circ_0016760 in vivo. Results Circ_0016760 and AKT3 were highly expressed in NSCLC tissue specimens and cell lines. Circ_0016760 interference suppressed cell proliferation, migration, and invasion and promoted the apoptosis of NSCLC cells. Circ_0016760 interacted with miR‐646 and negatively regulated its expression. MiR‐646 silencing partly counteracted circ_0016760 knockdown‐mediated influences in NSCLC cells. MiR‐646 bound to the AKT3 3′ untranslated region in NSCLC cells, and miR‐646 overexpression‐induced effects in NSCLC cells were partly overturned by the addition of AKT3 overexpression plasmid. Circ_0016760 silencing reduced the expression of AKT3 through enhancing miR‐646 expression. Circ_0016760 knockdown suppressed NSCLC tumor growth in vivo. Conclusion Circ_0016760 played an oncogenic role to promote the proliferation, migration, and invasion and restrained the apoptosis of NSCLC cells via miR‐646/AKT3 signaling.

BackgroundAutophagy, a key regulator of programmed cell death, is critical for maintaining the stability of the intracellular environment. Increasing evidence has revealed the clinical importance of interactions between autophagy and immune status in lung adenocarcinoma. The present study evaluated the potential of autophagy-immune-derived biomarkers to predict prognosis and therapeutic response in patients with lung adenocarcinoma.MethodsPatients from the GSE72094 dataset were randomized 7:3 to a training set and an internal validation set. Three independent cohorts, TCGA, GSE31210, and GSE37745, were used for external verification. Unsupervised hierarchical clustering based on autophagy- and immune-associated genes was used to identify autophagy- and immune-associated molecular patterns, respectively. Significantly prognostic autophagy-immune genes were identified by LASSO analysis and by univariate and multivariate Cox regression analyses. Differences in tumor immune microenvironments, functional pathways, and potential therapeutic responses were investigated to differentiate high-risk and low-risk groups.ResultsHigh autophagy status and high immune status were associated with improved overall survival. Autophagy and immune subtypes were merged into a two-dimensional index to characterize the combined prognostic classifier, with 535 genes defined as autophagy-immune-related differentially expressed genes (DEGs). Four genes (C4BPA, CD300LG, CD96, and S100P) were identified to construct an autophagy-immune-related prognostic risk model. Survival and receiver operating characteristic (ROC) curve analyses showed that this model was significantly prognostic of survival. Patterns of autophagy and immune genes differed in low- and high-risk patients. Enrichment of most immune infiltrating cells was greater, and the expression of crucial immune checkpoint molecules was higher, in the low-risk group. TIDE and immunotherapy clinical cohort analysis predicted that the low-risk group had more potential responders to immunotherapy. GO, KEGG, and GSEA function analysis identified immune- and autophagy-related pathways. Autophagy inducers were observed in patients in the low-risk group, whereas the high-risk group was sensitive to autophagy inhibitors. The expression of the four genes was assessed in clinical specimens and cell lines.ConclusionsThe autophagy-immune-based gene signature represents a promising tool for risk stratification in patients with lung adenocarcinoma, guiding individualized targeted therapy or immunotherapy.

In this paper, the material point method (MPM) is used to explore the influence of matrix (namely soil) in the soil-rock mixture (SRM) on the stability of SRM slope. Firstly, a typical slope model is established, and a series of circular stone blocks with different sizes are generated inside the slope, then the SRM slope model is established. Next, the gravity is linearly loaded. When it is completed, the kinetic damping is applied, and the kinetic energy of the SRM slope is set to 0 to obtain the initial state of the slope. Then, the stability of the SRM slope under different soil cohesions and internal friction angles is simulated by MPM. The simulation results show that the stability of the SRM slope is more affected by cohesion than the internal friction angle. When the SRM slope enters the large deformation stage, there are both translational sliding and rotational sliding modes in the slope. The translational sliding is mainly the soil above the slope surface, and the SRM under the slope occurs rotational sliding.