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Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress

Authors :
Amr. R. Ghanam
Shengwei Ke
Shujuan Wang
Ramy Elgendy
Chenyao Xie
Siqi Wang
Ran Zhang
Min Wei
Weiguang Liu
Jun Cao
Yan Zhang
Zhi Zhang
Tian Xue
Yong Zheng
Xiaoyuan Song
Source :
iScience, Vol 26, Iss 1, Pp 105740- (2023)
Publication Year :
2023
Publisher :
Elsevier, 2023.

Abstract

Summary: The function of the cancer-associated lncRNA Malat1 during aging is as-of-yet uncharacterized. Here, we show that Malat1 interacts with Nucleophosmin (NPM) in young mouse brain, and with Lamin A/C, hnRNP C, and KAP1 with age. RNA-seq and RT-qPCR reveal a persistent expression of Malat1_2 (the 3’isoform of Malat1) in Malat1Δ1 (5’-1.5 kb deletion) mouse retinas and brains at 1/4th level of the full-length Malat1, while Malat1_1 (the 5’isoform) in Malat1Δ2 (deletion of 3’-conserved 5.7 kb) at a much lower level, suggesting an internal promoter driving the 3’ isoform. The 1774 and 496 differentially expressed genes in Malat1Δ2 and Malat1Δ1 brains, respectively, suggest the 3’ isoform regulates gene expression in trans and the 5’ isoform in cis. Consistently, Malat1Δ2 mice show increased age-dependent retinal oxidative stress and corneal opacity, while Malat1Δ1 mice show no obvious phenotype. Collectively, this study reveals a physiological function of the lncRNA Malat1 3’-isoform during the aging process.

Details

Language :
English
ISSN :
25890042 and 04858107
Volume :
26
Issue :
1
Database :
Directory of Open Access Journals
Journal :
iScience
Publication Type :
Academic Journal
Accession number :
edsdoj.b04858107913410f8660b32aeb01f596
Document Type :
article
Full Text :
https://doi.org/10.1016/j.isci.2022.105740