37 results on '"Yinglin Peng"'
Search Results
2. A novel evaluation model of image registration for cone‐beam computed tomography guided lung cancer radiotherapy
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Yimei Liu, Meining Chen, Jianlan Fang, Liangjie Xiao, Songran Liu, Qiwen Li, Bo Qiu, Runda Huang, Jun Zhang, and Yinglin Peng
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clinic model ,cone beam computed tomography ,dice similarity coefficient ,image‐guided radiotherapy ,lung cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The aim of the study was to establish a weighted comprehensive evaluation model (WCEM) of image registration for cone‐beam computed tomography (CBCT) guided lung cancer radiotherapy that considers the geometric accuracy of gross target volume (GTV) and organs at risk (OARs), and assess the registration accuracy of different image registration methods to provide clinical references. Methods The planning CT and CBCT images of 20 lung cancer patients were registered using diverse algorithms (bony and grayscale) and regions of interest (target, ipsilateral, and body). We compared the coverage ratio (CR) of the planning target volume (PTVCT) to GTVCBCT, as well as the dice similarity coefficient (DSC) of the GTV and OARs, considering the treatment position across various registration methods. Furthermore, we developed a mathematical model to assess registration results comprehensively. This model was evaluated and validated using CRFs across four automatic registration methods. Results The grayscale registration method, coupled with the registration of the ipsilateral structure, exhibited the highest level of automatic registration accuracy, the DSC were 0.87 ± 0.09 (GTV), 0.71 ± 0.09 (esophagus), 0.74 ± 0.09 (spinal cord), and 0.91 ± 0.05 (heart), respectively. Our proposed WCEM proved to be both practical and effective. The results clearly indicated that the grayscale registration method, when applied to the ipsilateral structure, achieved the highest CRF score. The average CRF scores, excellent rates, good rate and qualification rates were 58 ± 26, 40%, 75%, and 85%, respectively. Conclusions This study successfully developed a clinically relevant weighted evaluation model for CBCT‐guided lung cancer radiotherapy. Validation confirmed the grayscale method's optimal performance in ipsilateral structure registration.
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- 2024
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3. Flavonoids from mulberry leaves inhibit fat production and improve fatty acid distribution in adipose tissue in finishing pigs
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Yingying Liu, Yinglin Peng, Chen Chen, Huibo Ren, Ji Zhu, Yuan Deng, Qingming Cui, Xionggui Hu, Jianhua He, Huali Li, Xinghui Zhu, Yulong Yin, Jun He, and Yi Xiao
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Finishing pig ,Mulberry leaf ,Flavonoid ,Lipid metabolism ,Fatty acid ,Lard ,Animal culture ,SF1-1100 - Abstract
This study evaluated the effects of flavonoids from mulberry leaves (FML) on plasma biochemical indices, serum activities of lipid metabolism-related enzymes, fat morphology, fatty acid composition, and lipid metabolism in different adipose tissues of finishing pigs. We used 120 Chinese hybrid barrows of Berkshire and Bama mini-pigs with an average initial body weight of 45.11 ± 4.23 kg. The pigs were randomly assigned to five treatment groups and fed a control diet based on corn, soybean meal, and wheat bran or a control diet supplemented with 0.02%, 0.04%, 0.08%, or 0.16% FML. Each experimental group had six replicates (pens), with four pigs per pen. After a 7-d adaptation period, the feeding trial was conducted for 58 d. Blood and adipose tissue samples were collected from 30 pigs (one pig per pen) at the end of the test. The results showed that FML supplementation significantly decreased the feed intake to body gain ratio, the plasma concentrations of total cholesterol and free fatty acids, and the serum activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (linear or quadratic effects, P
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- 2024
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4. Integrated analysis strategy of genome-wide functional gene mining reveals DKK2 gene underlying meat quality in Shaziling synthesized pigs
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Shuaihan He, Yubei Wang, Yabiao Luo, Mingming Xue, Maisheng Wu, Hong Tan, Yinglin Peng, Kejun Wang, and Meiying Fang
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Shaziling pigs ,Whole genome sequencing ,Selective sweep ,IBD analysis ,DKK2 ,Meat quality ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Shaziling pig is a well-known indigenous breed in China who has superior meat quality traits. However, the genetic mechanism and genomic evidence underlying meat quality characteristics of Shaziling pigs are still unclear. To explore and investigate the germplasm characteristics of Shaziling pigs, we totally analyzed 67 individual’s whole genome sequencing data for the first time (20 Shaziling pigs [S], 20 Dabasha pigs [DBS], 11 Yorkshire pigs [Y], 10 Berkshire pigs [BKX], 5 Basha pigs [BS] and 1 Warthog). Results A total of 2,538,577 SNPs with high quality were detected and 9 candidate genes which was specifically selected in S and shared in S to DBS were precisely mined and screened using an integrated analysis strategy of identity-by-descent (IBD) and selective sweep. Of them, dickkopf WNT signaling pathway inhibitor 2 (DKK2), the antagonist of Wnt signaling pathway, was the most promising candidate gene which was not only identified an association of palmitic acid and palmitoleic acid quantitative trait locus in PigQTLdb, but also specifically selected in S compared to other 48 Chinese local pigs of 12 populations and 39 foreign pigs of 4 populations. Subsequently, a mutation at 12,726-bp of DKK2 intron 1 (g.114874954 A > C) was identified associated with intramuscular fat content using method of PCR–RFLP in 21 different pig populations. We observed DKK2 specifically expressed in adipose tissues. Overexpression of DKK2 decreased the content of triglyceride, fatty acid synthase and expression of relevant genes of adipogenic and Wnt signaling pathway, while interference of DKK2 got contrary effect during adipogenesis differentiation of porcine preadipocytes and 3T3-L1 cells. Conclusions Our findings provide an analysis strategy for mining functional genes of important economic traits and provide fundamental data and molecular evidence for improving pig meat quality traits and molecular breeding.
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- 2024
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5. Multi-Sequence Fusion Network via Single- Sequence CycleGANs for Improved Synthetic CT in Nasopharyngeal Carcinoma Treatment Planning
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Yimei Liu, Meining Chen, Jun Zhang, Yixuan Wang, Huikuan Gu, Chong Zhao, Zhenyu Qi, Xiaowu Deng, Shuyu Wu, and Yinglin Peng
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Synthetic CT ,multiple MR sequence ,MRI guided radiotherapy ,cycle-consistent generative adversarial network ,nasopharyngeal carcinoma ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
To investigate the effect of different MR sequences on the accuracy of Cycle-consistent Generative Adversarial Network (CycleGAN) based synthetic CT (sCT) generation in nasopharyngeal carcinoma (NPC). In this work, three sequences of MR, included T1-weighted imaging (T1WI), T2-weighted imaging (T2WI), and T1 contrast-enhanced weighted imaging (T1WIC), and planning CT (PCT) images of 151 patients with NPC were collected. The number of training, verification, and test sets were 108, 16, and 27, respectively. Three unsupervised CycleGAN-based models were trained using different sequences (single channels) as inputs, and the synergistic fusion model were used multiple channels. To assess the precision of these models, we evaluated key metrics such as mean error (ME), mean absolute error (MAE), structural similarity index (SSIM), peak signal-to-noise ratio (PSNR), and dose distribution, comparing the PCT with the sCT generated by each model. Overall, The SCTT2 image generated by T2WI model achieved superior results than those of T1WIC and T1WI for a single-sequence model. Compared to the SCTT2, the quality of the SCTFusion image generated by the fusion model was further improved, with significant differences in the MAE, SSIM, and PNSR metrics (P $112.97\pm 9.69$ vs. $90.99\pm 9.64$ , P $0.86\pm 0.02$ vs. $0.89\pm 0.02$ , P $21.66\pm 0.64$ vs. $23.28\pm 0.87$ , P $\pm ~1.05$ % and 0.73%±0.73% for the fusion model. In conclusion, our findings reveal that the CycleGAN model, particularly when employing fusion MR sequences as input, offers the highest accuracy in synthetic CT generation. Notably, T2WI images stand out as a viable option for sCT prediction in clinical settings where acquisition sequences or times are limited.
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- 2024
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6. Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs
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Chen Chen, Yitong Chang, Yuan Deng, Qingming Cui, Yingying Liu, Huali Li, Huibo Ren, Ji Zhu, Qi Liu, and Yinglin Peng
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backfat ,daweizi (dwz) pigs ,pathway ,rna sequencing ,transcript profile ,yorkshire pigs ,Zoology ,QL1-991 - Abstract
Objective Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 downregulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.
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- 2023
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7. Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies
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Hongyu Zhu, Dehuan Xie, Ying Wang, Runda Huang, Xi Chen, Yiwei Yang, Bin Wang, Yinglin Peng, Jianxin Wang, Dexin Xiao, Dai Wu, Chao-Nan Qian, and Xiaowu Deng
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Ultra-high dose rate radiotherapy ,FLASH-RT ,Tumor control ,Immune response ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background/Purpose: Investigating the antitumor effect and intratumor as well as local immune response in breast cancer-bearing mice after MV X-ray ultra-high dose rate radiotherapy (FLASH-RT) and conventional dose rate radiotherapy (CONV-RT). Materials/Methods: Six-week-old female C57BL/6 mice were inoculated subcutaneously with Py8119 and Py230 breast tumor cells in the inguinal mammary gland and administered 10 Gy abdominal 6 MV X-ray FLASH-RT (125 Gy/s) or CONV-RT (0.2 Gy/s) 15 days after tumor inoculation. Tumor and spleen tissues were obtained at different time points post-irradiation (PI) for analysis of immune cell infiltration using flow cytometry and immunohistochemical (IHC) staining. Intestine tissues were collected 3 days PI to evaluate normal tissue damage and immune cell infiltration. Results: Both FLASH-RT and CONV-RT significantly delayed tumor growth. Flow cytometry showed increased CD8+/CD3 + and CD8+/CD4 + ratios, and IHC confirmed a similar increased CD8 + T cell infiltration at 2 weeks PI in Py8119 tumor tissues in both irradiation groups. No statistical difference was observed between the irradiation groups in terms of tumor growth and increased T cell infiltration in the tumor. Unexpectedly, significantly smaller spleen weight and substantially higher CD8+/CD3 + and lower CD4+/CD3 + ratios were observed in the spleens of the FLASH-RT group than in the spleens of the non-irradiated control and CONV-RT groups 4 weeks PI. Pathological analysis revealed severe red pulp expansion in several spleens from the CONV-RT group, but not in the spleens of the FLASH-RT group. Reduced intestinal damage, macrophage and neutrophil infiltration were observed in the FLASH-RT group compared with CONV-RT group. Conclusions: FLASH-RT and CONV-RT effectively suppressed tumor growth and promoted CD8 + T cell influx into tumors. FLASH-RT can induce different splenic immune responses and reduce radiation-induced damage in the spleen and intestine, which may potentially enhance the therapeutic ratio of FLASH-RT.
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- 2023
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8. Identification of crucial modules and genes associated with backfat tissue development by WGCNA in Ningxiang pigs
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Chen Chen, Huibo Ren, Huali Li, Yuan Deng, Qingming Cui, Ji Zhu, Siyang Zhang, Jine Yu, Huiming Wang, Xiaodan Yu, Shiliu Yang, Xionggui Hu, and Yinglin Peng
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Ningxiang pig ,backfat tissue ,different developmental stage ,WGCNA ,hub gene ,lipid metabolism ,Genetics ,QH426-470 - Abstract
Fat deposition is an economically important trait in pigs. Ningxiang pig, one of the four famous indigenous breeds in China, is characterized by high fat content. The underlying gene expression pattern in different developmental periods of backfat tissue remains unclear, and the purpose of this investigation is to explore the potential molecular regulators of backfat tissue development in Ningxiang pigs. Backfat tissue (three samples for each stage) was initially collected from different developmental stages (60, 120, 180, 240, 300, and 360 days after birth), and histological analysis and RNA sequencing (RNA-seq) were then conducted. Fragments per kilobase of transcript per million (FPKM) method was used to qualify gene expressions, and differentially expressed genes (DEGs) were identified. Furthermore, strongly co-expressed genes in modules, which were named by color, were clustered by Weighted gene co-expression network analysis (WGCNA) based on dynamic tree cutting algorithm. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment were subsequently implemented, and hub genes were described in each module. Finally, QPCR analysis was employed to validate RNA-seq data. The results showed that adipocyte area increased and adipocyte number decreased with development of backfat tissue. A total of 1,024 DEGs were identified in five comparison groups (120 days vs. 60 days, 180 days vs. 120 days, 240 days vs. 180 days, 300 days vs. 240 days, and 360 days vs. 300 days). The turquoise, red, pink, paleturquoise, darkorange, and darkgreen module had the highest correlation coefficient with 60, 120, 180, 240, 300, and 360 days developmental stage, while the tan, black and turquoise module had strong relationship with backfat thickness, adipocyte area, and adipocyte number, respectively. Thirteen hub genes (ACSL1, ACOX1, FN1, DCN, CHST13, COL1A1, COL1A2, COL6A3, COL5A1, COL14A1, OAZ3, DNM1, and SELP) were recognized. ACSL1 and ACOX1 might perform function in the early developmental stage of backfat tissue (60 days), and FN1, DCN, COL1A1, COL1A2, COL5A1, COL6A3, and COL14A1 have unignorable position in backfat tissue around 120 days developmental stage. Besides, hub genes SELP and DNM1 in modules significantly associated with backfat thickness and adipocyte area might be involved in the process of backfat tissue development. These findings contribute to understand the integrated mechanism underlying backfat tissue development and promote the progress of genetic improvement in Ningxiang pigs.
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- 2023
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9. Genome-Wide Association Study and Identification of Candidate Genes for Intramuscular Fat Fatty Acid Composition in Ningxiang Pigs
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Qinghua Zeng, Hu Gao, Shishu Yin, Yinglin Peng, Fang Yang, Yawei Fu, Xiaoxiao Deng, Yue Chen, Xiaohong Hou, Qian Wang, Zhao Jin, Gang Song, Jun He, Yulong Yin, and Kang Xu
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longissimus dorsi ,saturated fatty acids ,monounsaturated fatty acids ,polyunsaturated fatty acids ,GWAS ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Ningxiang pigs exhibit a diverse array of fatty acids, making them an intriguing model for exploring the genetic underpinnings of fatty acid metabolism. We conducted a genome-wide association study using a dataset comprising 50,697 single-nucleotide polymorphisms (SNPs) and samples from over 600 Ningxiang pigs. Our investigation yielded novel candidate genes linked to five saturated fatty acids (SFAs), four monounsaturated fatty acids (MUFAs), and five polyunsaturated fatty acids (PUFAs). Significant associations with SFAs, MUFAs, and PUFAs were found for 37, 21, and 16 SNPs, respectively. Notably, some SNPs have significant PVE, such as ALGA0047587, which can explain 89.85% variation in Arachidic acid (C20:0); H3GA0046208 and DRGA0016063 can explain a total of 76.76% variation in Elaidic Acid (C18:1n-9(t)), and the significant SNP ALGA0031262 of Arachidonic acid (C20:4n-6) can explain 31.76% of the variation. Several significant SNPs were positioned proximally to previously reported genes. In total, we identified 11 candidate genes (hnRNPU, CEPT1, ATP1B1, DPT, DKK1, PRKG1, EXT2, MEF2C, IL17RA, ITGA1 and ALOX5), six candidate genes (ALOX5AP, MEDAG, ISL1, RXRB, CRY1, and CDKAL1), and five candidate genes (NDUFA4L2, SLC16A7, OTUB1, EIF4E and ROBO2) associated with SFAs, MUFAs, and PUFAs, respectively. These findings hold great promise for advancing breeding strategies aimed at optimizing meat quality and enhancing lipid metabolism within the intramuscular fat (IMF) of Ningxiang pigs.
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- 2023
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10. Mulberry leaf powder regulates antioxidative capacity and lipid metabolism in finishing pigs
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Yingying Liu, Yinghui Li, Yi Xiao, Yinglin Peng, Jianhua He, Chen Chen, Dingfu Xiao, Yulong Yin, and Fengna Li
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Mulberry ,Xiangcun black pig ,Antioxidative capacity ,Fatty acid ,Lipid metabolism ,Animal culture ,SF1-1100 - Abstract
This study evaluated the potential of mulberry leaf powder as an unconventional feed material for finishing pigs by assessing the growth performance, antioxidative properties, fatty acid profile, and lipid metabolism in 180 Xiangcun black pigs. Pigs with an initial body weight (BW) of 71.64 ± 1.46 kg were randomly assigned to 5 treatment groups, including the control diet and 4 experimental diets. The corn, soybean meal, and wheat bran in the control diet were partly replaced by 3%, 6%, 9%, or 12% mulberry leaf powder in experimental diets. There were 6 replicates (pens) of 6 pigs per replicate in each treatment. Blood and muscle samples were collected after the 50-day feed experiment. Compared with the control group, the 3%, 6%, and 9% mulberry diets had no adverse effect (P > 0.05) on the growth performance of pigs. The serum glutathione peroxidase activity and glutathione concentration increased linearly (P
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- 2021
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11. Multivariate NTCP Model of Hypothyroidism After Intensity-Modulated Radiotherapy for Nasopharyngeal Carcinoma
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Guanzhu Shen, Yinglin Peng, Jian Li, Haijun Wu, Guangshun Zhang, Chong Zhao, and Xiaowu Deng
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nasopharyngeal carcinoma ,intensity-modulated radiotherapy ,hypothyroidism ,EQD2 ,NTCP mode ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
ObjectiveTo evaluate the incidence of hypothyroidism in patients with nasopharyngeal carcinoma after intensity-modulated radiotherapy (IMRT), analyze its correlation with multiple influencing factors such as thyroid exposure dose, thyroid volume, and gender, and construct a multivariate-based normal tissue complication probability (NTCP) model for the occurrence of hypothyroidism after IMRT.Materials and MethodsThe thyroid hormone levels of patients at different points in time before and after radiotherapy were tested, and statistics on the incidence of hypothyroidism after treatment were obtained. The dose-volume data of patients’ thyroids were converted into EQD2 equivalent dose values. The correlation between hypothyroidism after radiotherapy and thyroid exposure dose, thyroid volume, gender, and other factors was analyzed, and an NTCP model was constructed.ResultsA total of 69 patients with nasopharyngeal carcinoma were enrolled in this study. Twelve months after radiotherapy, a total of 24 patients (34.8%) developed hypothyroidism. Univariate analysis and multivariate analysis revealed that the average thyroid dose and thyroid volume are the most important factors affecting hypothyroidism after radiotherapy. The NTCP model constructed based on the average dose and thyroid volume has a good degree of fit.ConclusionThe volume and average dose of the thyroid gland are the key factors affecting the occurrence of hypothyroidism in patients with nasopharyngeal carcinoma after radiotherapy. The NTCP model constructed based on multivariate construction suggests that reducing the average dose of the thyroid to the greatest extent is an effective way to protect thyroid functions.
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- 2021
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12. Analysis of measurement electrode location in bladder urine monitoring using electrical impedance
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Yaning Li, Yinglin Peng, Xin Yang, Shipei Lu, Jinwu Gao, Chengguang Lin, and Rihui Li
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Computational simulation ,Electrical impedance ,Urine monitor ,Medical technology ,R855-855.5 - Abstract
Abstract Purpose The aim of this study was to document more appropriate electrode location of a four-electrode-based electrical impedance technology in the monitoring of bladder filling, and to characterize the relationship between bladder filling duration and the measured electrical impedances. Methods A simulation study, based on a 2-dimension computational model, was conducted to determine the preferable locations of excitation and measurement electrodes in a conventional four-electrode setup. A human observation study was subsequently performed on eight healthy volunteers during natural bladder urine accumulation to validate the result of the simulation study. The correlation between the bladder filling time and the measured electrical impedance values was evaluated. Results The preferable location of measurement electrodes was successively validated by the model simulation study and human observation study. Result obtained via the selected electrodes location revealed a significant negative correlation (R = 0.916 ± 0.059, P
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- 2019
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13. Comparison of Different Combinations of Irradiation Mode and Jaw Width in Helical Tomotherapy for Nasopharyngeal Carcinoma
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Jun Zhang, Yinglin Peng, Shouliang Ding, Jinhan Zhu, Yimei Liu, Meining Chen, Wenzhao Sun, Linghong Zhou, and Xiaowu Deng
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helical tomotherapy ,nasopharyngeal carcinoma ,dynamic jaw ,irradiation mode ,jaw width ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Purpose: To aid in the selection of a suitable combination of irradiation mode and jaw width in helical tomotherapy (HT) for the treatment of nasopharyngeal carcinoma (NPC).Materials and Methods: Twenty patients with NPC who underwent radiotherapy were retrospectively selected. Four plans using a jaw width of 2.5 or 5-cm in dynamic jaw (DJ) or fix jaw (FJ) modes for irradiation were designed (2.5DJ, 2.5FJ, 5.0DJ, and 5.0FJ). The dose parameters of planning target volume (PTV) and organs at risk (OARs) of the plans were compared and analyzed, as well as the beam on time (BOT) and monitor unit (MU). The plans in each group were ranked by scoring the doses received by the OARs and the superity was assessed in combination with the planned BOT and MU.Results: The prescribed dose coverage of PTV met the clinical requirements for all plans in the four groups. The groups using a 2.5-cm jaw width or a DJ mode provided better protection to most OARs, particularly for those at the longitudinal edges of the PTV (P < 0.05). The 2.5DJ group had the best ranking for OAR-dose, followed by the 2.5FJ and 5.0DJ groups with a same score. The BOT and MU of the groups using a 5.0-cm jaw width reduced nearly 45% comparing to those of the 2.5-cm jaw groups.Conclusion: 2.5DJ has the best dose distribution, while 5.0DJ has satisfactory dose distribution and less BOT and MU that related to the leakage dose. Both 2.5DJ or 5DJ were recommended for HT treatment plan for NPC based on the center workload.
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- 2020
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14. A Study on Changes of Carcass, Bones and Muscles of Ningxiang Pigs.
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Wei Zhang, Yinglin Peng, Qingming Cui, Yitong Chang, Yuan Deng, Chen Chen, and Yingying Liu
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MEAT quality , *LARGE intestine , *BIVARIATE analysis , *MUSCLE growth , *SMALL intestine , *ERECTOR spinae muscles - Abstract
Background: Ningxiang pigs are known for tasty meat and high intramuscular fat content. Currently, the slow growth rate and low lean meat rate of Ningxiang pigs have greatly restricted the development of its market. Therefore, the objective of this study is to explore the changes in carcass traits, meat quality, visceral organ weight indices, bones and muscles development with slaughter ages of Ningxiang pigs. Methods: The experiment used 0, 60, 120, 180, 240, 300 and 360 days old Ningxiang pigs as the research objects, and 6 castrated male pigs in each group were randomly selected. The carcass traits, meat quality, visceral organs weights and skeletal muscle weights were measured. The correlation between carcass traits and meat quality were analyzed by bivariate analysis, and the regression equations between slaughter ages and these indicators were established. Result: The results showed that pre-slaughter weight and weights of carcass, head, feet, skeleton and muscle, loin eye area, cooking yield, marbling and the large intestine weight index at 0 d, backfat thickness at 60 d, fat percentages at 60 and 120 d and the lean meat percentage at 240 d, redness at 120 d of Ningxiang pigs were lowest, the heart, lung, kidney and small intestine weight percentages were highest at 0 d (P<0.05). Carcass traits and meat quality had different significant correlation at different slaughter ages. So the indices of carcass, meat quality, bones and muscles of Ningxiang pigs changed with slaughter ages. In general, the overall performance of 300 d Ningxiang pigs is better. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Long-term Survivals, Toxicities and the Role of Chemotherapy in Early-Stage Nasopharyngeal Carcinoma Patients Treated with Intensity-Modulated Radiation Therapy: A Retrospective Study with 15-Year Follow-up
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Xiao Xiao, Wei-Xiong Xia, Shaomin Huang, Jingjing Miao, Fei Han, Lin Wang, Xiang Guo, Yan-Qun Xiang, Chong Zhao, Yinglin Peng, Boyu Chen, Xiaowu Deng, Huageng Huang, Xing Lv, Yingshan Liang, Manyi Zhu, and Weiwei Xiao
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Adult ,Male ,Intensity-modulated radiation therapy ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Early-stage ,Gastroenterology ,Disease-Free Survival ,Internal medicine ,otorhinolaryngologic diseases ,Mucositis ,medicine ,Humans ,Chemotherapy ,Long-term outcomes ,Stage (cooking) ,Aged ,Retrospective Studies ,Nasopharyngeal Carcinoma ,Toxicity ,business.industry ,Head and Neck cancer ,Nasopharyngeal Neoplasms ,Retrospective cohort study ,Middle Aged ,medicine.disease ,Progression-Free Survival ,Radiation therapy ,stomatognathic diseases ,medicine.anatomical_structure ,Oncology ,Nasopharyngeal carcinoma ,Cervical lymph nodes ,Female ,Original Article ,Radiotherapy, Intensity-Modulated ,business ,Follow-Up Studies - Abstract
Purpose This study was aimed to investigate long-term survivals and toxicities of early-stage nasopharyngeal carcinoma (NPC) in endemic area, evaluating the role of chemotherapy in stage II patients.Materials and Methods Totally 187 patients with newly diagnosed NPC and restaged American Joint Committee on Cancer/ International Union Against Cancer 8th T1-2N0-1M0 were retrospectively recruited. All received intensity-modulated radiotherapy (IMRT)±chemotherapy (CT) from 2001 to 2010.Results With 15.7-year median follow-up, 10-year locoregional recurrence-free survival, distant metastasis-free survival (DMFS), disease-specific survival (DSS), and overall survival (OS) were 93.3%, 93.5%, 92.9% and 88.2%, respectively. Multivariable analyses showed cervical lymph nodes positive and pre-treatment prognostic nutritional index ≥ 52.0 could independently predict DMFS (p=0.036 and p=0.011), DSS (p=0.014 and p=0.026), and OS (p=0.002 and p < 0.001); Charlson comorbidity index < 3 points could predict DSS (p=0.011); age > 45 years (p=0.002) and pre-treatment lactate dehydrogenase ≥ 240 U/L (p < 0.001) predicted OS. No grade 4 late toxicity happened; grade 3 late toxicities included subcutaneous fibrosis (4.3%), deafness or otitis (4.8%), skin dystrophy (2.1%), and xerostomia (1.1%). No differences on survivals were shown between IMRT+CT vs. IMRT alone in stage II patients, even in T2N1M0 (p > 0.05). Unsurprising, patients in IMRT+CT had more acute gastrointestinal reaction, myelosuppression, mucositis, late ear toxicity, and cranial nerve injury (all p < 0.05) than IMRT alone group.Conclusion Superior tumor control and satisfying long-term outcomes could be achieved with IMRT in early-stage NPC with mild late toxicities. As CT would bring more toxicities, it should be carefully performed to stage II patients.
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- 2022
16. Comparison of intratumor and local immune response between MV X-ray FLASH and conventional radiotherapies
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Hongyu Zhu, Dehuan Xie, Ying Wang, Runda Huang, Xi Chen, Yiwei Yang, Bin Wang, Yinglin Peng, Jianxin Wang, Dexin Xiao, Dai Wu, Chao-Nan Qian, and Xiaowu Deng
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Oncology ,Radiology, Nuclear Medicine and imaging - Abstract
Investigating the antitumor effect and intratumor as well as local immune response in breast cancer-bearing mice after MV X-ray ultra-high dose rate radiotherapy (FLASH-RT) and conventional dose rate radiotherapy (CONV-RT).Six-week-old female C57BL/6 mice were inoculated subcutaneously with Py8119 and Py230 breast tumor cells in the inguinal mammary gland and administered 10 Gy abdominal 6 MV X-ray FLASH-RT (125 Gy/s) or CONV-RT (0.2 Gy/s) 15 days after tumor inoculation. Tumor and spleen tissues were obtained at different time points post-irradiation (PI) for analysis of immune cell infiltration using flow cytometry and immunohistochemical (IHC) staining. Intestine tissues were collected 3 days PI to evaluate normal tissue damage and immune cell infiltration.Both FLASH-RT and CONV-RT significantly delayed tumor growth. Flow cytometry showed increased CD8+/CD3 + and CD8+/CD4 + ratios, and IHC confirmed a similar increased CD8 + T cell infiltration at 2 weeks PI in Py8119 tumor tissues in both irradiation groups. No statistical difference was observed between the irradiation groups in terms of tumor growth and increased T cell infiltration in the tumor. Unexpectedly, significantly smaller spleen weight and substantially higher CD8+/CD3 + and lower CD4+/CD3 + ratios were observed in the spleens of the FLASH-RT group than in the spleens of the non-irradiated control and CONV-RT groups 4 weeks PI. Pathological analysis revealed severe red pulp expansion in several spleens from the CONV-RT group, but not in the spleens of the FLASH-RT group. Reduced intestinal damage, macrophage and neutrophil infiltration were observed in the FLASH-RT group compared with CONV-RT group.FLASH-RT and CONV-RT effectively suppressed tumor growth and promoted CD8 + T cell influx into tumors. FLASH-RT can induce different splenic immune responses and reduce radiation-induced damage in the spleen and intestine, which may potentially enhance the therapeutic ratio of FLASH-RT.
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- 2022
17. Patient-specific daily updated deep learning auto-segmentation for MRI-guided adaptive radiotherapy
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Zhenjiang Li, Wei Zhang, Baosheng Li, Jian Zhu, Yinglin Peng, Chengze Li, Jennifer Zhu, Qichao Zhou, and Yong Yin
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Oncology ,Radiology, Nuclear Medicine and imaging ,Hematology - Abstract
Deep Learning (DL) technique has shown great potential but still has limited success in online contouring for MR-guided adaptive radiotherapy (MRgART). This study proposed a patient-specific DL auto-segmentation (DLAS) strategy using the patient's previous images and contours to update the model and improve segmentation accuracy and efficiency for MRgART.A prototype model was trained for each patient using the first set of MRI and corresponding contours as inputs. The patient-specific model was updated after each fraction with all the available fractional MRIs/contours, and then used to predict the segmentation for the next fraction. During model training, a variant was fitted under consistency constraints, limiting the differences in the volume, length and centroid between the predictions for the latest MRI within a reasonable range. The model performance was evaluated for both organ-at-risks and tumors auto-segmentation for a total of 6 abdominal/pelvic cases (each with at least 8 sets of MRIs/contours) underwent MRgART through Dice Similarity Coefficient (DSC) and 95% Hausdorff Distance (HD95), and was compared with deformable image registration (DIR) and frozen DL model (no updating after pre-training). The contouring time was also recorded and analyzed.The proposed model achieved superior performance with higher mean DSC (0.90, 95 % CI: 0.88-0.95), as compared to DIR (0.63, 95 %CI: 0.59-0.68) and frozen DL models (0.74, 95 % CI: 0.71-0.79). As for tumors, the proposed method yielded a median DSC of 0.95, 95 % CI: 0.94-0.97, and a median HD95 of 1.63 mm, 95 % CI: 1.22 mm-2.06 mm. The contouring time was reduced significantly (p 0.05) using the proposed method (73.4 ± 6.5 secs) compared to the manual process (12 ∼ 22 mins). The online ART time was reduced to 1650 ± 274 seconds with the proposed method, as compared to 3251.8 ± 447 seconds using the original workflow.The proposed patient-specific DLAS method can significantly improve the segmentation accuracy and efficiency for longitudinal MRIs, thereby facilitating the routine practice of MRgART.
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- 2022
18. Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs
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Chen Chen, Yitong Chang, Yuan Deng, Qingming Cui, Yingying Liu, Huali Li, Huibo Ren, Ji Zhu, Qi Liu, and Yinglin Peng
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General Veterinary ,Physiology ,Genetics ,Animal Science and Zoology ,Food Science - Abstract
Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition,Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed.Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 downregulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis.Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.
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- 2022
19. Radioprotective effect of X-ray abdominal FLASH irradiation: Adaptation to oxidative damage and inflammatory response may be benefiting factors
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Hongyu Zhu, Dehuan Xie, Yiwei Yang, Shaomin Huang, Xingwang Gao, Yinglin Peng, Bin Wang, Jianxin Wang, Dexin Xiao, Dai Wu, Changzhi Li, Chenghua Li, Chao‐Nan Qian, and Xiaowu Deng
- Subjects
Mice ,Oxidative Stress ,X-Rays ,Animals ,Cytokines ,Mice, Nude ,General Medicine ,Reactive Oxygen Species - Abstract
Ultrahigh dose-rate irradiation (FLASH-IR) was reported to be efficient in tumor control while reducing normal tissue radiotoxicity. However, the mechanism of such phenomenon is still unclear. Besides, the FLASH experiments using high energy X-ray, the most common modality in clinical radiotherapy, are rarely reported. This study aims to investigate the radiobiological response using 6 MV X-ray FLASH-IR or conventional dose-rate IR (CONV-IR).The superconducting linac of Chengdu THz Free Electron Laser (CTFEL) facility was used for FLASH-IR, a diamond radiation detector and a CeBrThe mean dose rate of 150 Gy/s and instantaneous dose rate of 5.5 × 10The radioprotective effect of 6 MV X-ray FLASH-IR was observed. The differences in inflammatory responses and redox status between the two groups may be the factors responsible for reduced radiotoxicities following FLASH-IR. Further studies are required to thoroughly evaluate the impact of ROS on FLASH effect.
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- 2022
20. Improved accuracy of auto-segmentation of organs at risk in radiotherapy planning for nasopharyngeal carcinoma based on fully convolutional neural network deep learning
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Yinglin, Peng, Yimei, Liu, Guanzhu, Shen, Zijie, Chen, Meining, Chen, Jingjing, Miao, Chong, Zhao, Jincheng, Deng, Zhenyu, Qi, and Xiaowu, Deng
- Subjects
Cancer Research ,Oncology ,Oral Surgery - Abstract
We examined a modified encoder-decoder architecture-based fully convolutional neural network, OrganNet, for simultaneous auto-segmentation of 24 organs at risk (OARs) in the head and neck, followed by validation tests and evaluation of clinical application.Computed tomography (CT) images from 310 radiotherapy plans were used as the experimental data set, of which 260 and 50 were used as the training and test sets, respectively. An improved U-Net architecture was established by introducing a batch normalization layer, residual squeeze-and-excitation layer, and unique organ-specific loss function for deep learning training. The performance of the trained network model was evaluated by comparing the manual-delineation and the STAPLE contour of 10 physicians from different centers.Our model achieved good segmentation in all 24 OARs in nasopharyngeal cancer radiotherapy plan CT images, with an average Dice similarity coefficient of 83.75%. Specifically, the mean Dice coefficients in large-volume organs (brainstem, spinal cord, left/right parotid glands, left/right temporal lobes, and left/right mandibles) were 84.97% - 95.00%, and in small-volume organs (pituitary, lens, optic nerve, and optic chiasma) were 55.46% - 91.56%. respectively. Using the STAPLE contours as standard contour, the OrganNet achieved comparable or better DICE in organ segmentation then that of the manual-delineation as well.The established OrganNet enables simultaneous automatic segmentation of multiple targets on CT images of the head and neck radiotherapy plans, effectively improves the accuracy of U-Net based segmentation for OARs, especially for small-volume organs.
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- 2023
21. Mulberry leaf powder regulates antioxidative capacity and lipid metabolism in finishing pigs
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Fengna Li, Yinghui Li, Yingying Liu, Jianhua He, Yulong Yin, Yi Xiao, Dingfu Xiao, Yinglin Peng, and Chen Chen
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Soybean meal ,Body weight ,SF1-1100 ,03 medical and health sciences ,chemistry.chemical_compound ,Animal science ,Food Animals ,Antioxidative capacity ,Original Research Article ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Bran ,0402 animal and dairy science ,food and beverages ,Fatty acid ,Lipid metabolism ,04 agricultural and veterinary sciences ,Glutathione ,Glutathione peroxidase activity ,040201 dairy & animal science ,Animal culture ,chemistry ,Animal Science and Zoology ,Mulberry leaf ,Mulberry ,Xiangcun black pig - Abstract
This study evaluated the potential of mulberry leaf powder as an unconventional feed material for finishing pigs by assessing the growth performance, antioxidative properties, fatty acid profile, and lipid metabolism in 180 Xiangcun black pigs. Pigs with an initial body weight (BW) of 71.64 ± 1.46 kg were randomly assigned to 5 treatment groups, including the control diet and 4 experimental diets. The corn, soybean meal, and wheat bran in the control diet were partly replaced by 3%, 6%, 9%, or 12% mulberry leaf powder in experimental diets. There were 6 replicates (pens) of 6 pigs per replicate in each treatment. Blood and muscle samples were collected after the 50-day feed experiment. Compared with the control group, the 3%, 6%, and 9% mulberry diets had no adverse effect (P > 0.05) on the growth performance of pigs. The serum glutathione peroxidase activity and glutathione concentration increased linearly (P
- Published
- 2020
22. The Clinicopathological Characteristics, MRI Features, And Radiation Dose Of Radio-Induced Brain Necrosis In Nasopharyngeal Carcinoma Patients
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Jun Dong, Minggu Zhong, Yinglin Peng, Siyue Mao, YuKun Chen, Hao Duan, Suwen Wu, Yi Wu, Jiyong Gu, Zhibin Li, Enming Cui, Jun Wen, Shulan Liu, YuJie Liu, Ronglue Wei, Junying Liu, Zhongqin Luan, Zize Feng, Yilong Peng, Wenguang Zhang, and Jiajun Dong
- Abstract
Background: Radio-induced brain necrosis is a late-onset radiotherapy complication, especially in nasopharyngeal carcinoma (NPC) patients. We presented the clinicopathological characteristics, dynamic changes of MRI features, and radiation dose in the areas of radio-induced brain necrosis, which will shed light on preventing this severe radiotherapy complication. Methods: We retrospectively collecting and reanalyzing clinical, imaging, radiation plans and pathological data from 48 NPC patients diagnosed with radio-induced brain necrosis and underwent craniotomy. To calculate the radiation dose in the areas of radio-induced brain necrosis, we reviewed the radiation plan of each patient and delineated the volume of the radio-induced brain necrosis. We also mapped the dynamic changes of magnetic resonance imaging (MRI) features and performed CD3, CD31, CD68, CD11b, Ki67, terminal deoxynucleotidyl transferase-dUTP nick end labeling (TUNEL) and HE staining on radio-induced brain necrosis specimens to observe pathological changes. Results: The mean latency period for radio-induced brain necrosis was 9.23 years. According to the radiotherapy plans, the mean radiation dose for NPC was 7041±553 cGy. The mean dose to the radio-induced brain necrosis area was 5684.57±409.99 cGy. The necrotic areas exhibited high-intensity signals on T2-weighted images (WIs) and low-intensity signals on T1WIs over time. HE staining showed that the necrotic areas contained irregular fibers and inflammatory cells. The immunohistochemical results showed CD3(+), CD31(+), CD68(+), CD11b(+), Ki67(+), and TUNEL(+) cells in radio-induced brain necrosis specimens. Conclusions: NPC patients who underwent radiotherapy and survived more than 5 years with hyperintense signals in temporal lobes in the T2WI should be paid close attention to radio-induced brain necrosis. A radiation dose no more than 5684.57±409.99 cGy in temporal lobes of NPC patients is recommended.
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- 2022
23. Regulatory roles of circRNAs in adipogenesis and lipid metabolism: emerging insights into lipid‐related diseases
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Chen Chen, Huibo Ren, Yinglin Peng, Ji Zhu, Yuan Deng, Jianbo Zuo, Xing Zhang, Xionggui Hu, Yingying Liu, and Qingming Cui
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0301 basic medicine ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Adipose Tissue, White ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Adipose tissue ,Computational biology ,Biology ,Fatty Acid-Binding Proteins ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Adipose Tissue, Brown ,Diabetes Mellitus ,medicine ,Animals ,Humans ,Obesity ,RNA, Messenger ,Molecular Biology ,Adipogenesis ,Lipid metabolism ,RNA, Circular ,Cell Biology ,Lipid Metabolism ,medicine.disease ,Fatty Liver ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Fatty Acid Synthases ,Steatosis ,Biogenesis ,Signal Transduction - Abstract
Disorder of lipid metabolism has become an urgent health problem that brings about a variety of metabolic syndromes, including hepatic steatosis, adipose tissue dysfunction, diabetes and obesity. Circular RNAs (circRNAs), a class of emerging RNA molecules with unique structure and extensive effects, have been verified to participate in various biological programs through distinct mechanisms, especially in lipid-related processes. In this review, the biogenesis, characteristics, and functional mechanisms of circRNAs are discussed. Furthermore, the methods for circRNA identification and expression profiles of circRNAs associated with adipogenesis and lipid metabolism are described. Additionally, we emphasize the regulatory roles of circRNAs in adipogenesis, lipid metabolism, and lipid-related diseases. Finally, the diagnostic and therapeutic potential of circRNAs is highlighted, showing potential for the clinical application of circRNAs in the treatment of lipid-related diseases in the near future.
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- 2020
24. Impact on xerostomia for nasopharyngeal carcinoma patients treated with superficial parotid lobe-sparing intensity-modulated radiation therapy (SPLS-IMRT): A prospective phase II randomized controlled study
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Huageng Huang, Jingjing Miao, Xiao Xiao, Jiang Hu, Guangshun Zhang, Yinglin Peng, Shunzhen Lu, Yingshan Liang, Shaomin Huang, Fei Han, Xiaowu Deng, Chong Zhao, and Lin Wang
- Subjects
Nasopharyngeal Carcinoma ,Oncology ,Humans ,Parotid Gland ,Radiology, Nuclear Medicine and imaging ,Nasopharyngeal Neoplasms ,Hematology ,Radiotherapy, Intensity-Modulated ,Prospective Studies ,Xerostomia - Abstract
To compare the incidence of xerostomia in nasopharyngeal carcinoma (NPC) patients treated with superficial parotid lobe-sparing intensity-modulated radiation therapy (SPLS-IMRT) and conventional IMRT (C-IMRT).Patients with histologically confirmed NPC who met the eligibility criteria were randomly assigned to receive either SPLS-IMRT or C-IMRT. The primary endpoint was the incidence of xerostomia at 12 months post-IMRT. The secondary endpoints included the xerostomia questionnaire (XQ) score, unstimulated salivary flow rate (USFR), stimulated salivary flow rate (SSFR), and survival outcomes.Ninety patients were enrolled. Eighty-two patients were included for xerostomia analysis (42 in the SPLS-IMRT group and 40 in the C-IMRT group). At 12 months post-IMRT, the incidence of xerostomia in the SPLS-IMRT group was significantly lower than that in the C-IMRT group (83.4% vs 95.0%; P = 0.007), especially the grade 3 xerostomia (0% vs 12.5%; P 0.001). The median change in XQ score was similar between the two groups (11.9 points vs 14.1 points; P = 0.194). There was a significantly higher median fractional USFR (0.67 vs 0.35; P = 0.024) and SSFR (0.66 vs 0.32; P = 0.021) in the SPLS-IMRT group than the C-IMRT group. The 3-year LRRFS, DMFS, and OS in the SPLS-IMRT and C-IMRT groups were 92.5% vs 90.9%, 83.8% vs 81.7%, and 88.9% vs 88.2% (all P 0.05).SPLS-IMRT significantly reduced the incidence of xerostomia at 12 months post-IMRT in NPC by recovering parotid gland function earlier than C-IMRT, without compromising survivals. Phase III clinical trials are warranted. (ClinicalTrials.gov, number NCT05020067).
- Published
- 2021
25. Comparison of combinations of irradiation techniques and jaw conditions in intensity-modulated radiotherapy for lung cancer
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Qiwen Li, Yinglin Peng, Li Chen, Junyue Shi, Jun Zhang, Zhenghuan Li, Qingyuan Zhang, and Qinghe Peng
- Subjects
Organs at Risk ,medicine.medical_specialty ,Lung Neoplasms ,medicine.medical_treatment ,VMAT ,Pulmonary function testing ,Metastasis ,adaptive jaw ,medicine ,Humans ,Radiation Oncology Physics ,Radiology, Nuclear Medicine and imaging ,Jaw tracking ,IMRT ,Lung cancer ,Instrumentation ,Retrospective Studies ,Radiation ,Lung ,business.industry ,Radiotherapy Planning, Computer-Assisted ,Radiotherapy Dosage ,respiratory system ,medicine.disease ,Peripheral ,respiratory tract diseases ,Radiation therapy ,stomatognathic diseases ,lung cancer ,medicine.anatomical_structure ,jaw tracking ,Mediastinal lymph node ,Radiology ,Radiotherapy, Intensity-Modulated ,business - Abstract
Purpose To assist in the selection of a suitable combination of an irradiation technique and jaw condition in intensity-modulated radiotherapy (IMRT) and volumetric-modulated arc radiotherapy (VMAT) for lung cancer treatment plans. Materials and methods Thirty patients with lung cancer who underwent radiotherapy were enrolled retrospectively. They were categorized as having central lung cancer, peripheral lung cancer with mediastinal lymph node metastasis (peripheral E lung cancer), and peripheral lung cancer without mediastinal lymph node metastasis (peripheral N lung cancer). Four treatment plans were designed for each patient: fixed jaw and adaptive jaw IMRT technique (FJ-IMRT and JA-IMRT), and fixed jaw and jaw tracking VMAT technique (FJ-VMAT and JT-VMAT). The dose parameters of the four group plans were compared and analyzed. Results Compared to FJ-IMRT, JA-IMRT significantly reduced the mean dose (Dmean ) and volume percentage of 5 Gy (V5Gy ) of the total lung in central and peripheral N lung cancer. Similarly, compared to FJ-VMAT, JT-VMAT provided better protection to most organs at risk (OARs), particularly for total lung and heart. In comparison with IMRT, VMAT significantly improved the conformity index (CI) of the planning target volume for the three lung cancer classifications, and it reduced the dose of almost all OARs except V5Gy and Dmean of the total lung. Moreover, the mean monitor units of the VMAT groups were far lower than the IMRT groups. Conclusion Based on the dosimetric findings and considering clinical data published on lung and heart side effects, we propose recommendations on the preferred treatment technique based on tumor location and pulmonary function. For central lung cancer with normal pulmonary function, we advise JT-VMAT techniques. Conversely, for central lung cancer with poor pulmonary function, we recommend JA-IMRT techniques. We advocate JA-IMRT for peripheral E lung cancer. For peripheral N lung cancer, JT-VMAT techniques are strongly recommended.
- Published
- 2021
26. Computed Tomography-Based Evaluation of Volume and Position Changes of the Target Region and Organs at Risk During Radiotherapy for Esophageal Cancer: A Pilot Study
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Xiaowu Deng, Qiwen Li, Jun Zhang, Hui Liu, Ya-Ru Ma, Bo Qiu, Yinglin Peng, Yimei Liu, Guanzhu Shen, Li-Rong Fu, and Meining Chen
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Cancer Research ,medicine.medical_treatment ,Planning target volume ,Computed tomography ,Volume change ,03 medical and health sciences ,0302 clinical medicine ,medicine ,esophageal cancer ,geometric center deviation ,RC254-282 ,Original Research ,medicine.diagnostic_test ,business.industry ,Isocenter ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Esophageal cancer ,medicine.disease ,Gross tumor volume ,Radiation therapy ,volume changed ratio ,Oncology ,adaptive radiotherapy ,030220 oncology & carcinogenesis ,target retraction ,business ,Nuclear medicine ,030217 neurology & neurosurgery ,Volume (compression) - Abstract
ObjectiveTo analyze changes in volume and position of target regions and organs at risk (OARs) during radiotherapy for esophageal cancer patients.MethodsOverall, 16 esophageal cancer patients who underwent radiotherapy, including 10 cases of intensity-modulated radiation therapy (IMRT) and six of three-dimensional conformal radiotherapy (3D-CRT), were enrolled. The prescription doses for the planning target volumes (PTVs) were as follows: PTV1, 64 Gy/32 fractions; and PTV2, 46 Gy/23 fractions. Repeat computed tomography (CT) was performed for patients after the 5th, 10th, 15th, 20th, and 25th fractions. Delineation of the gross tumor volume (GTV) and OAR volume was determined using five repeat CTs performed by the same physician. The target and OAR volumes and centroid positions were recorded and used to analyze volume change ratio (VCR), center displacement (ΔD), and changes in the distance from the OAR centroid positions to the planned radiotherapy isocenter (distance to isocenter, DTI) during treatment.ResultsNo patient showed significant changes in target volume (TV) after the first week of radiotherapy (five fractions). However, TV gradually decreased over the following weeks, with the rate slowing after the fourth week (40 Gy). The comparison of TV from baseline to 40 Gy (20 fractions) showed that average GTVs decreased from 130.7 ± 63.1 cc to 92.1 ± 47.2 cc, with a VCR of −29.21 ± 13.96% (ppConclusionDuring radiotherapy for esophageal cancer, Targets and OARs change significantly in volume and position during the 2nd–4th weeks. Image-guidance and evaluation of dosimetric changes are recommended for these fractions of treatment to appropriate adjust treatment plans.
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- 2021
27. Dietary mulberry leaf powder affects growth performance, carcass traits and meat quality in finishing pigs
- Author
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Yinghui Li, Yinglin Peng, Yulong Yin, Ruilin Huang, Yingying Liu, Chen Chen, Fengna Li, Dingfu Xiao, and Jianhua He
- Subjects
Male ,Inosine monophosphate ,Meat ,Swine ,040301 veterinary sciences ,0403 veterinary science ,chemistry.chemical_compound ,Animal science ,Food Animals ,Fodder ,Animals ,Animal nutrition ,Cholinesterase ,biology ,Body Weight ,0402 animal and dairy science ,Albumin ,04 agricultural and veterinary sciences ,Animal Feed ,040201 dairy & animal science ,Diet ,Plant Leaves ,chemistry ,Dietary Supplements ,Body Composition ,biology.protein ,Urea ,Uric acid ,Animal Nutritional Physiological Phenomena ,Animal Science and Zoology ,Composition (visual arts) ,Morus - Abstract
This study was conducted to evaluate the effect of mulberry leaves as an alternative source of protein on growth performance, carcass traits and meat quality in finishing pigs. A total of 180 Xiangcun Black pigs were randomly assigned to five treatment groups with six pens of six pigs per pen. The pigs were provided with a basal diet or a diet contained 3%, 6%, 9% or 12% of mulberry leaf powder during a 50-day experiment period. The results showed that dietary mulberry leaf powder had no negative effect on growth performance in Xiangcun Black pigs, except in the 12% mulberry group, where final body weight and average daily gain decreased (p < .05) and feed to gain ratio of the pigs increased (p < .05). Dietary mulberry inclusion decreased (quadratic, p < .05) the back fat thickness, fibre mean cross-sectional area (CSA) in the longissimus dorsi (LD) muscle and mRNA expression levels of myosin heavy chain (MyHC) IIb in LD and biceps femoris (BF) muscles, while increased (linear or quadratic, p < .05) the plasma concentration of albumin, levels of crude protein (CP), inosine monophosphate (IMP) and several amino acids in muscle tissues. When compared with the other groups, the 9% mulberry diet increased (p < .05) loin-eye area and contents of CP and IMP in muscles, while decreased (p < .05) plasma activity of cholinesterase and concentrations of uric acid and urea. The 6% mulberry diet had the lowest fibre mean CSA and shear force and increased total fibre number of the LD muscle, when compared with the other groups. These results suggest that including mulberry in the diet at
- Published
- 2019
28. Long non-coding RNAs regulation in adipogenesis and lipid metabolism: Emerging insights in obesity
- Author
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Luo Xuan, Yinglin Peng, Xing Zhang, Yingying Liu, Jianbo Zuo, Qingming Cui, and Chen Chen
- Subjects
0301 basic medicine ,Adipose Tissue, White ,Future application ,Adipose tissue ,Computational biology ,Biology ,Mice ,03 medical and health sciences ,Adipose Tissue, Brown ,Brown adipocyte differentiation ,medicine ,Animals ,Homeostasis ,Humans ,Obesity ,Cholesterol homeostasis ,Adipogenesis ,Thermogenesis ,Lipid metabolism ,Cell Biology ,Lipid Metabolism ,medicine.disease ,030104 developmental biology ,RNA, Long Noncoding - Abstract
Obesity is a widespread health problem that brings about various adipose tissue dysfunctions. The balance of energy storage and energy expenditure is critical for normal fat accumulation and lipid metabolism. Therefore, understanding the molecular basis of adipogenesis and thermogenesis is essential to maintain adipose development and lipid homeostasis. Increasing evidence demonstrated that lncRNAs (long non-coding RNAs), a class of non-protein coding RNAs of >200 nucleotides in length, are identified as key regulators in obesity-related biological processes through diverse regulatory mechanisms. In this review, we concentrate on recent and relevant studies on the roles of lncRNAs in regulation of white adipogenesis, brown adipocyte differentiation and lipid metabolism. In addition, the diagnostic and therapeutic potential of lncRNAs is highlighted, and that will make recommendations for the future application of lncRNAs in the treatment of obesity.
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- 2018
29. Magnetic resonance-based synthetic computed tomography images generated using generative adversarial networks for nasopharyngeal carcinoma radiotherapy treatment planning
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Xingwang Gao, A. Qin, Xiaowu Deng, Jingjing Miao, Meining Chen, Yinglin Peng, Huikuan Gu, S. Chen, Chong Zhao, Yimei Liu, and Zhen-Yu Qi
- Subjects
Magnetic Resonance Spectroscopy ,Computer science ,medicine.medical_treatment ,Computed tomography ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Hounsfield scale ,Imrt planning ,medicine ,Image Processing, Computer-Assisted ,Humans ,Radiology, Nuclear Medicine and imaging ,Nasopharyngeal Carcinoma ,medicine.diagnostic_test ,business.industry ,Radiotherapy Planning, Computer-Assisted ,Magnetic resonance imaging ,Nasopharyngeal Neoplasms ,Radiotherapy Dosage ,Hematology ,Radiotherapy treatment planning ,medicine.disease ,Magnetic Resonance Imaging ,Radiation therapy ,Oncology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Mr images ,Nuclear medicine ,business ,Tomography, X-Ray Computed - Abstract
Background and purpose To investigate the feasibility of synthesizing computed tomography (CT) images from magnetic resonance (MR) images using generative adversarial networks (GANs) for nasopharyngeal carcinoma (NPC) intensity-modulated radiotherapy (IMRT) planning. Materials and methods Conventional T1-weighted MR images and CT images were acquired from 173 NPC patients. The MR and CT images of 28 patients were randomly chosen as the independent tested set. The remaining images were used to build a conditional GAN (cGAN) and a cycle-consistency GAN (cycleGAN). A U-net was used as the generator in cGAN, whereas a residual-Unet was used as the generator in cycleGAN. The cGAN was trained using the deformable registered MR-CT image pairs, whereas the cycleGAN was trained using the unregistered MR and CT images. The generated synthetic CT (SCT) images from cGAN and cycleGAN were compared with the true CT images with respect to their Hounsfield Unit (HU) discrepancy and dosimetric accuracy for NPC IMRT plans. Results The mean absolute errors within the body were 69.67 ± 9.27 HU and 100.62 ± 7.39 HU for the cGAN and cycleGAN, respectively. The 2%/2-mm γ passing rates were (98.68 ± 0.94)% and (98.52 ± 1.13)% for the cGAN and cycleGAN, respectively. Meanwhile, the absolute dose discrepancies within the regions of interest were (0.49 ± 0.24)% and (0.62 ± 0.36)%, respectively. Conclusion Both cGAN and cycleGAN could swiftly generate accurate SCT volume images from MR images, with high dosimetric accuracy for NPC IMRT planning. cGAN was preferable if high-quality MR-CT image pairs were available.
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- 2019
30. miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2
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Chen Chen, Yinglin Peng, Shengcai Fu, Xie Julan, Ji Zhu, Huibo Ren, Yuan Deng, and Xionggui Hu
- Subjects
0301 basic medicine ,Peroxisome proliferator-activated receptor gamma ,Physiology ,Lipolysis ,Adipocytes, White ,Peroxisome proliferator-activated receptor ,Adipose tissue ,Biochemistry ,Cell Line ,Mice ,03 medical and health sciences ,Genes, Reporter ,3T3-L1 Cells ,Cricetinae ,Lipid droplet ,Animals ,Point Mutation ,RNA, Small Interfering ,3' Untranslated Regions ,Triglycerides ,chemistry.chemical_classification ,Adipogenesis ,Gene Expression Regulation, Developmental ,3T3-L1 ,Lipid metabolism ,Lipid Droplets ,General Medicine ,Cell biology ,PPAR gamma ,MicroRNAs ,030104 developmental biology ,chemistry ,CCAAT-Enhancer-Binding Proteins ,RNA ,RNA Interference ,Biomarkers ,Transcription Factors - Abstract
Differentiation of adipocytes and their aggregation to adipose tissue are critical for mammalian growth and development. MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that play important roles in adipogenesis and lipid metabolism. miR-128-3p may contribute to adipose tissue development according to the previous studies. However, the role of miR-128-3p in the process of preadipocyte differentiation and lipid metabolism is not yet understood. The purpose of this research was to investigate the biological function and molecular mechanism of miR-128-3p in 3T3-L1 cells. In the present study, we found that miR-128-3p was downregulated during the process of 3T3-L1 preadipocyte differentiation. Overexpression of miR-128-3p obstructed the expressions of adipogenic marker genes as well as the lipid droplets accumulation and triglyceride content, suggesting the importance of miR-128-3p for adipogenesis. Moreover, miR-128-3p could lead to the retardation of cell proliferation in 3T3-L1 preadipocytes. Further evidences showed that, as a negative regulator of adipogenesis, miR-128-3p could directly target peroxisome proliferator-activated receptor γ (Pparg) which resulted in the suppression of 3T3-L1 preadipocyte differentiation, and miR-128-3p could also bind with SERTA domain containing 2 (Sertad2) which drove triglyceride hydrolysis and lipolysis. In addition, inhibition of Sertad2 with siRNA displayed the same effects as overexpression of miR-128-3p. Our research demonstrated that miR-128-3p impeded 3T3-L1 adipogenesis by targeting Pparg and Sertad2, resulting in the obstruction of preadipocyte differentiation and promotion of lipolysis. Taken together, this study offers profound insight into the mechanism of miRNA-mediated adipogenesis and lipid metabolism.
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- 2018
31. Improving two-stage thermophilic-mesophilic anaerobic co-digestion of swine manure and rice straw by digestate recirculation
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Benyi Xiao, Zhi Yu, Yunping Han, Jun Wu, Rongzhan Liu, Xiangyu Chen, Wenzhe Zhang, Hong Chen, and Yinglin Peng
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Environmental Engineering ,Swine ,Methanogenesis ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Bioreactors ,Animals ,Environmental Chemistry ,Organic matter ,Anaerobiosis ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Energy recovery ,biology ,Chemistry ,Public Health, Environmental and Occupational Health ,Oryza ,General Medicine ,General Chemistry ,Methanosarcina ,Pulp and paper industry ,biology.organism_classification ,Pollution ,Manure ,020801 environmental engineering ,Waste treatment ,Biofuels ,Digestate ,Digestion ,Methane ,Mesophile - Abstract
The anaerobic co-digestion (coAD) of swine manure (SM) and rice straw (RS) is appealing for renewable energy recovery and waste treatment worldwidely. Improving its performance is very important for its application. In this study, long-term semi-continuous experiments were conducted to evaluate the improving effects of digestate recirculation on the performance, energy recovery, and microbial community of two-stage thermophilic-mesophilic coAD of swine manure (SM) and rice straw (RS). The experimental results indicated that the coAD systems of SM and RS (mixing ratio of 3:1) with or without digestate recirculation could not realize phase separation. The reactors of both coAD systems were characterized by pH values ranging from 7.74 to 7.85, methane production as 0.41 ± 0.02 and 0.44 ± 0.03 L/L/d, and stable operation. Notably, digestate recirculation increased total methane production, organic matter removal, and reaction rate of the coAD system by 9.92 ± 5.08, 5.22 ± 1.94, and 9.73–12.60%, respectively. Digestate recirculation improved the performance of the coAD by significantly increasing the abundance of Methanosarcina (from 4.1% to 7.5%–10.7% and 35.7%) and decreasing that of Methanothermobacter (from 94.2% to 87.3%–83.6% and 56.8%). Thus, the main methanogenesis pathway of the coAD system was changed by digestate recirculation and the methane production was effectively improved. Although the energy input of the coAD system increased by 30.26%, digestate recirculation improved the energy balance of the total system by 6.83%.
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- 2021
32. Long-term Survivals, Toxicities and the Role of Chemotherapy in Early-Stage Nasopharyngeal Carcinoma Patients Treated with Intensity-Modulated Radiation Therapy: A Retrospective Study with 15-Year Follow-up.
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Lin Wang, Jingjing Miao, Huageng Huang, Boyu Chen, Xiao Xiao, Manyi Zhu, Yingshan Liang, Weiwei Xiao, Shaomin Huang, Yinglin Peng, Xiaowu Deng, Xing Lv, Weixiong Xia, Yanqun Xiang, Xiang Guo, Fei Han, and Chong Zhao
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NASOPHARYNX cancer ,RADIOTHERAPY ,LACTATE dehydrogenase ,INTENSITY modulated radiotherapy ,CRANIAL nerves - Abstract
Purpose This study was aimed to investigate long-term survivals and toxicities of early-stage nasopharyngeal carcinoma (NPC) in endemic area, evaluating the role of chemotherapy in stage II patients. Materials and Methods Totally 187 patients with newly diagnosed NPC and restaged American Joint Committee on Cancer/International Union Against Cancer 8th T1-2N0-1M0 were retrospectively recruited. All received intensity-modulated radiotherapy (IMRT)±chemotherapy (CT) from 2001 to 2010. Results With 15.7-year median follow-up, 10-year locoregional recurrence-free survival, distant metastasis-free survival (DMFS), disease-specific survival (DSS), and overall survival (OS) were 93.3%, 93.5%, 92.9% and 88.2%, respectively. Multivariable analyses showed cervical lymph nodes positive and pre-treatment prognostic nutritional index = 52.0 could independently predict DMFS (p=0.036 and p=0.011), DSS (p=0.014 and p=0.026), and OS (p=0.002 and p < 0.001); Charlson comorbidity index < 3 points could predict DSS (p=0.011); age > 45 years (p=0.002) and pre-treatment lactate dehydrogenase = 240 U/L (p < 0.001) predicted OS. No grade 4 late toxicity happened; grade 3 late toxicities included subcutaneous fibrosis (4.3%), deafness or otitis (4.8%), skin dystrophy (2.1%), and xerostomia (1.1%). No differences on survivals were shown between IMRT+CT vs. IMRT alone in stage II patients, even in T2N1M0 (p > 0.05). Unsurprising, patients in IMRT+CT had more acute gastrointestinal reaction, myelosuppression, mucositis, late ear toxicity, and cranial nerve injury (all p < 0.05) than IMRT alone group. Conclusion Superior tumor control and satisfying long-term outcomes could be achieved with IMRT in early-stage NPC with mild late toxicities. As CT would bring more toxicities, it should be carefully performed to stage II patients. [ABSTRACT FROM AUTHOR]
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- 2022
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33. Analysis of measurement electrode location in bladder urine monitoring using electrical impedance
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Shipei Lu, Yinglin Peng, Li Yaning, Xin Yang, Chengguang Lin, Rihui Li, and Jinwu Gao
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Adult ,Male ,lcsh:Medical technology ,Materials science ,Urine volume ,0206 medical engineering ,Urinary Bladder ,Biomedical Engineering ,02 engineering and technology ,Urine ,Biomaterials ,Young Adult ,Healthy volunteers ,Electric Impedance ,Humans ,Radiology, Nuclear Medicine and imaging ,Urine monitor ,Computer Simulation ,Electrical impedance ,Electrodes ,Monitoring, Physiologic ,Radiological and Ultrasound Technology ,Research ,General Medicine ,020601 biomedical engineering ,Bladder filling ,Healthy Volunteers ,lcsh:R855-855.5 ,Electrode location ,Electrode ,Electrical impedance technique ,Computational simulation ,Female ,Tomography, X-Ray Computed ,Biomedical engineering - Abstract
Purpose The aim of this study was to document more appropriate electrode location of a four-electrode-based electrical impedance technology in the monitoring of bladder filling, and to characterize the relationship between bladder filling duration and the measured electrical impedances. Methods A simulation study, based on a 2-dimension computational model, was conducted to determine the preferable locations of excitation and measurement electrodes in a conventional four-electrode setup. A human observation study was subsequently performed on eight healthy volunteers during natural bladder urine accumulation to validate the result of the simulation study. The correlation between the bladder filling time and the measured electrical impedance values was evaluated. Results The preferable location of measurement electrodes was successively validated by the model simulation study and human observation study. Result obtained via the selected electrodes location revealed a significant negative correlation (R = 0.916 ± 0.059, P
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- 2019
34. [Accuracy of different image registration methods in image-guided adaptive brachytherapy for cervical cancer]
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Qinghe, Peng, Yinglin, Peng, Jinhan, Zhu, Mingzhan, Cai, and Linghong, Zhou
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Organs at Risk ,基础研究 ,Radiotherapy Planning, Computer-Assisted ,Brachytherapy ,Humans ,Uterine Cervical Neoplasms ,Female ,Radiotherapy Dosage ,Tomography, X-Ray Computed ,Software ,Radiotherapy, Image-Guided - Abstract
OBJECTIVE: To compare the accuracy of different methods for image registration in image-guided adaptive brachytherapy (IGABT) for cervical cancer. METHODS: The last treatment planning CT images (CT1) and the first treatment planning CT images (CT2) were acquired from 15 patients with cervical cancer and registered with different match image qualities (retained/removed catheter source in images) and different match regions [target only (S Group)/ interested organ structure (M Group)/body (L Group)] in Velocity3.2 software. The dice similarity coefficient (DSC) between the clinical target volumes (CTV) of the CT1 and CT2 images (CTVCT1 and CTVCT2, respectively) and between the organs-at-risk (OAR) of the two imaging datasets (OARCT1 and OARCT2, respectively) were used to evaluate the image registration accuracy. RESULTS: The auto-segmentation volume of the catheter source using Velocity software based on the CT threshold was the closest to the actual volume within the CT value range of 1700-1800 HU. In the retained group, the DSC for the OARs of was better than or equal to that of the removed group, and the DSC value of the rectum was significantly improved (P < 0.05). For comparison of different match regions, the high-risk target volume (HRCTV) and the low-risk target volume (IRCTV) had the best precision for registration of the target area, which was significantly greater than that of M group and L group (P < 0.05). The M group had better registration accuracy of the target area and the best accuracy for the OARs. The DSC values of the bladder and rectum were significantly better than those of the other two groups (P < 0.05). CONCLUSION: The CT value range of 1700-1800 HU is optimal for automatic image segmentation using Velocity software. Automatic segmentation and shielding the volume of the catheter source can improve the image quality. We recommend the use of interested organ structures regions for image registration in image-guided adaptive brachytherapy for cervical cancer.
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- 2018
35. Growth performance, carcass characteristics, meat quality and chemical composition of the Shaziling pig and its crossbreeds
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Chen Chen, Ji Zhu, Huibo Ren, Yuan Deng, Xing Zhang, Yingying Liu, Qingming Cui, Xionggui Hu, Jianbo Zuo, Bin Chen, Maisheng Wu, and Yinglin Peng
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0301 basic medicine ,General Veterinary ,media_common.quotation_subject ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Biology ,040201 dairy & animal science ,Breed ,03 medical and health sciences ,030104 developmental biology ,Animal science ,Animal Science and Zoology ,Quality (business) ,Chemical composition ,Purebred ,media_common - Abstract
The Shaziling pig, an indigenous breed reared in Hunan province in China, is characterized by good meat quality and strong ability of resistance for general diseases. The objective of this study was to compare properties of growth performance, carcass characteristics, meat quality, and chemical composition among purebred Shaziling, Berkshire, Yorkshire pigs and their crossbreeds Yorkshire × (Berkshire × Shaziling) (Y × (B × S)), Berkshire × (Yorkshire × Shaziling) (B × (Y × S)) pigs. The results showed that Shaziling pigs had lower average daily gain (ADG) (P
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- 2021
36. Retrospective dosimetry study of intensity-modulated radiation therapy for nasopharyngeal carcinoma: measurement-guided dose reconstruction and analysis
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Xiaowu Deng, Wenzhao Sun, Li Chen, Dan Dan Zhang, Bin Wang, Yinglin Peng, and Kang Dehua
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Adult ,Male ,lcsh:Medical physics. Medical radiology. Nuclear medicine ,Intensity-modulated radiation therapy ,lcsh:R895-920 ,medicine.medical_treatment ,Dose distribution ,lcsh:RC254-282 ,Imaging phantom ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Dosimetry ,Radiology, Nuclear Medicine and imaging ,Radiometry ,Aged ,Retrospective Studies ,Nasopharyngeal Carcinoma ,business.industry ,Radiotherapy Planning, Computer-Assisted ,Research ,Carcinoma ,Correction ,Nasopharyngeal Neoplasms ,Radiotherapy Dosage ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Quality assurance ,Radiation therapy ,Negative deviation ,Oncology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Dose reconstruction ,Gamma pass rate ,Female ,Radiotherapy, Intensity-Modulated ,Nuclear medicine ,business - Abstract
Background Conventional phantom-based planar dosimetry (2D-PBD) quality assurance (QA) using gamma pass rate (GP (%)) is inadequate to reflect clinically relevant dose error in intensity-modulated radiation therapy (IMRT), owing to a lack of information regarding patient anatomy and volumetric dose distribution. This study aimed to evaluate the dose distribution accuracy of IMRT delivery for nasopharyngeal carcinoma (NPC), which passed the 2D-PBD verification, using a measurement-guided 3D dose reconstruction (3D-MGR) method. Methods Radiation treatment plans of 30 NPC cases and their pre-treatment 2D-PBD data were analyzed. 3D dose distribution was reconstructed on patient computed tomography (CT) images using the 3DVH software and compared to the treatment plans. Global and organ-specific dose GP (%), and dose-volume histogram (DVH) deviation of each structure was evaluated. Interdependency between GP (%) and the deviation of the volumetric dose was studied through correlation analysis. Results The 3D-MGR achieved global GP (%) similar to conventional 2D-PBD in the same criteria. However, structure-specific GP (%) significantly decreased under stricter criteria, including the planning target volume (PTV). The average deviation of all inspected dose volumes (DV) and volumetric dose (VD) parameters ranged from − 2.93% to 1.17%, with the largest negative deviation in V100% of the PTVnx of − 15.66% and positive deviation in D1cc of the spinal cord of 6.66%. There was no significant correlation between global GP (%) of 2D-PBD or 3D-MGR and the deviation of the most volumetric dosimetry parameters (DV or VD), when the Pearson’s coefficient value of 0.8 was used for correlation evaluation. Conclusion Even upon passing the pre-treatment phantom based dosimetric QA, there could still be risk of dose error like under-dose in PTVnx and overdose in critical structures. Measurement-guided 3D volumetric dosimetry QA is recommended as the more clinically efficient verification for the complicated NPC IMRT. Electronic supplementary material The online version of this article (10.1186/s13014-018-0993-2) contains supplementary material, which is available to authorized users.
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- 2018
37. miR-135a-5p inhibits 3T3-L1 adipogenesis through activation of canonical Wnt/β-catenin signaling
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Chen Chen, Yongdong Peng, Yinglin Peng, Jian Peng, and Siwen Jiang
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Peroxisome proliferator-activated receptor gamma ,Adenomatous polyposis coli ,Active Transport, Cell Nucleus ,Biology ,Fatty Acid-Binding Proteins ,Cell Line ,Proto-Oncogene Proteins c-myc ,Mice ,Endocrinology ,3T3-L1 Cells ,Cricetinae ,Enhancer binding ,microRNA ,Adipocytes ,Animals ,Cyclin D1 ,RNA, Small Interfering ,Wnt Signaling Pathway ,Molecular Biology ,beta Catenin ,Adipogenesis ,Wnt signaling pathway ,3T3-L1 ,Cell biology ,PPAR gamma ,Wnt Proteins ,MicroRNAs ,Fatty acid synthase ,Adenomatous Polyposis Coli ,Adipose Tissue ,Gene Expression Regulation ,Biochemistry ,CCAAT-Enhancer-Binding Proteins ,biology.protein ,RNA Interference ,Fatty Acid Synthases - Abstract
MicroRNAs are endogenous, conserved, and non-coding small RNAs that function as post-transcriptional regulators of fat development and adipogenesis. Adipogenic marker genes, such as CCAAT/enhancer binding protein α (Cebpa), peroxisome proliferator-activated receptor γ (Pparg), adipocyte fatty acid binding protein (Ap2), and fatty acid synthase (Fas), are regarded as the essential transcriptional regulators of preadipocyte differentiation and lipid storage in mature adipocytes. Canonical Wnt/β-catenin signaling is recognized as a negative molecular switch during adipogenesis. In the present work we found that miR-135a-5p is markedly downregulated during the process of 3T3-L1 preadipocyte differentiation. Overexpression of miR-135a-5p impairs the expressions of adipogenic marker genes as well as lipid droplet accumulation and triglyceride content, indicating the importance of miR-135a-5p for adipogenic differentiation and adipogenesis. Further studies show that miR-135a-5p directly targets adenomatous polyposis coli (Apc), contributes to the translocation of β-catenin from cytoplasm to nucleus, and then activates the expressions of cyclin D1 (Ccnd1) and Cmyc, indicating the induction of canonical Wnt/β-catenin signaling. In addition, inhibition of APC with siRNA exhibits the same effects as overexpression of miR-135a-5p. Our findings demonstrate that miR-135a-5p suppresses 3T3-L1 preadipocyte differentiation and adipogenesis through the activation of canonical Wnt/β-catenin signaling by directly targeting Apc. Taken together, these results offer profound insights into the adipogenesis mechanism and the development of adipose tissue.
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- 2014
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