1,022 results on '"Yang Chang Wu"'
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2. Glutathione S‐transferase omega class 1 (GSTO1)‐associated large extracellular vesicles are involved in tumor‐associated macrophage‐mediated cisplatin resistance in bladder cancer
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Yi‐Cheng Pan, Pei‐Yi Chu, Ching‐Chan Lin, Ching‐Yun Hsieh, Wei‐Yu Hsu, Lie‐Fen Shyur, Juan‐Cheng Yang, Wei‐Chao Chang, and Yang‐Chang Wu
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bladder cancer ,cisplatin resistance ,extracellular vesicle ,GSTO1 ,tumor‐associated macrophage ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Bladder cancer poses a significant challenge to chemotherapy due to its resistance to cisplatin, especially at advanced stages. Understanding the mechanisms behind cisplatin resistance is crucial for improving cancer therapy. The enzyme glutathione S‐transferase omega class 1 (GSTO1) is known to be involved in cisplatin resistance in colon cancer. This study focused on its role in cisplatin resistance in bladder cancer. Our analysis of protein expression in bladder cancer cells stimulated by secretions from tumor‐associated macrophages (TAMs) showed a significant increase in GSTO1. This prompted further investigation into the role of GSTO1 in bladder cancer. We found a strong correlation between GSTO1 expression and cisplatin resistance. Mechanistically, GSTO1 triggered the release of large extracellular vesicles (EVs) that promoted cisplatin efflux, thereby reducing cisplatin–DNA adduct formation and enhancing cisplatin resistance. Inhibition of EV release effectively counteracted the cisplatin resistance associated with GSTO1. In conclusion, GSTO1‐mediated EV release may contribute to cisplatin resistance caused by TAMs in bladder cancer. Strategies to target GSTO1 could potentially improve the efficacy of cisplatin in treating bladder cancer.
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- 2024
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3. Raf/ERK/Nrf2 signaling pathway and MMP-7 expression involvement in the trigonelline-mediated inhibition of hepatocarcinoma cell migration
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Jung Chun Liao, Kun Tsung Lee, Bang Jau You, Chia Lin Lee, Wen Te Chang, Yang Chang Wu, and Hong-Zin Lee
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trigonelline ,hepatocarcinoma Hep3B cells ,migration ,Pisum sativum ,chemotherapeutic agent ,Raf/ERK/Nrf2 signaling pathway ,MMP-7 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Background: Trigonelline occurs in many dietary food plants and has been found to have anti-carcinogenic activity. Trigonelline is also found in coffee which is one of the most widely consumed beverages. Many epidemiological studies have reported that coffee consumption has an inverse relationship with the risk of cirrhosis or hepatocellular carcinoma. It would be interesting to investigate whether trigonelline is an ideal chemoprevent agent to prevent cancer progression. Methods: The protein expression was performed by western blotting. The trigonelline content in snow pea (Pisum sativum) was analyzed by high-performance liquid chromatography (HPLC). The migratory activity of human hepatocarcinoma cells (Hep3B) was assessed by using a wound migration assay. The percentage of each phase in the cell cycle was analyzed on a FACScan flow cytometer. Gene expression was detected by real-time reverse transcriptase-polymerase chain reaction techniques. Native gel analysis was performed to analyze the activity of superoxide dismutase (SOD), catalase and glutathione peroxidase. Results: According to the data of HPLC analysis, P. sativum, which is a popular vegetable, has relatively high content of trigonelline. Our findings suggest that trigonelline is an efficient compound for inhibiting Hep3B cell migration. Trigonelline inhibited the migration of hepatoma cells at concentrations of 75–100 µM without affecting proliferation. Raf/ERK/Nrf2 protein levels and further downstream antioxidative enzymes activity, such as SOD, catalase, and glutathione peroxidase, significantly decreased after treatment with 100 µM of trigonelline for 24 h. The migration inhibition of trigonelline is also related to its ability to regulate the matrix metalloproteinases 7 (MMP-7) gene expression. Conclusions: In this study, protein kinase Cα (PKCα) and Raf/ERK/Nrf2 signaling pathway and MMP-7 gene expression were involved in the trigonelline-mediated migration inhibition of Hep3B cells. We also demonstrated that trigonelline inhibits Hep3B cell migration through downregulation of nuclear factor E2-related factor 2–dependent antioxidant enzymes activity. This study analyzed the trigonelline content in a popular vegetable, snow pea, as a representative proof to prove that trigonelline is often found in the daily intake of food. Our finding suggested that trigonelline should be a useful chemopreventive agent derived from the daily intake of food to prevent cancer progression.
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- 2015
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4. Isolated from Antrodia cinnamomea, 25S-antcin K suppresses the production of inflammatory cytokines and cartilage degradation in rheumatoid arthritis
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Shan-Chi Liu, Tung-Ying Wu, Trung-Loc Ho, Chun-Hao Tsai, Wen-Hui Chung, Yen-You Lin, Yang-Chang Wu, and Chih-Hsin Tang
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Rheumatoid arthritis ,Antrodia cinnamomea ,25S-antcin K ,miR-374b ,Synovial fibroblasts ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Rheumatoid arthritis (RA) is a well-known autoimmune disorder associated with joint pain, swelling, cartilage and bone degradation, as well as deformity. In cellular and animal model investigations, extracts from Antrodia cinnamomea, a traditional medicinal fungus used in Taiwan, demonstrate anti-inflammatory properties. The 25(S) ergostane stereo-isomer of antcin K, known as 25S-antcin K, was previously isolated from A. cinnamomea and was found to exhibit a more potent effect than antcin K. In the current study, we examined the anti-RA effects of 25S-antcin K. 25S-antcin K abolishes IL-1β-induced promotion of TNF-α, CCL2, and VEGF arthritic factors production by inhibiting AMPK, Akt, and p38 pathways and enhancing miR-374b expression. Importantly, administration of 25S-antcin K also antagonizes collagen-induced arthritis-induced RA symptoms, such as inflammation, cartilage degradation, and bone erosion in vivo. These results provide a novel avenue, suggesting that 25S-antcin K is a potential candidate for developing RA therapy agents.
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- 2024
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5. Application of response surface methodology and quantitative NMR for the optimum extraction, characterization, and quantitation of Antrodia cinnamomea triterpenoids
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Chi-Ying Li, Yang-Chang Wu, Fang-Rong Chang, Mohamed El-Shazly, Ying-Chi Du, Chi-Yu Lu, Tsai-Hui Duh, and Tung-Ying Wu
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Medicine ,Science - Abstract
Abstract Antrodia cinnamomea (AC) is a treasured Asian medicinal mushroom, which has attracted attention due to recent research on its effectiveness in targeting a variety of serious ailments such as cancer and liver diseases. Among different A. cinnamomea constituents, triterpenoids are regarded as the most therapeutically attractive components because of their anti-inflammatory and cytotoxic activities. In the present study, we proposed a mathematical and statistical extraction protocol to evaluate the concentrations of total ergostane and lanostane triterpenoid derivatives from the ethanolic extract of the wild fruiting bodies of A. cinnamomea (EEAC) by utilizing response surface methodology (RSM) and quantitative NMR (qNMR) approaches. The optimum response surface model showed that the variations of the investigated response variables reached more than 90%, suggesting that the developed model is accurate in explaining response variability. Furthermore, the EEAC major characteristic triterpenoids were quantified through the comparison of the HPLC-tandem MS results with those of the qNMR results. The precision of the used techniques was also evaluated. The experimental design of the EEAC optimum extraction procedure obtained by using RSM and qNMR enabled accurate characterization and quantitation of A. cinnamomea triterpenoids.
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- 2023
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6. Umbelliferone and eriodictyol suppress the cellular entry of SARS-CoV-2
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Fang-Ju Cheng, Chien-Yi Ho, Tzong-Shiun Li, Yeh Chen, Yi-Lun Yeh, Ya-Ling Wei, Thanh Kieu Huynh, Bo-Rong Chen, Hung-Yu Ko, Chen-Si Hsueh, Ming Tan, Yang-Chang Wu, Hui-Chi Huang, Chih-Hsin Tang, Chia-Hung Chen, Chih-Yen Tu, and Wei-Chien Huang
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Artemisia argyi ,Eriodictyol ,Umbelliferone ,TMPRSS2 ,ACE2 ,SARS-CoV-2 variants ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Artemisia argyi (A. argyi), also called Chinese mugwort, has been widely used to control pandemic diseases for thousands of years since ancient China due to its anti-microbial infection, anti-allergy, and anti-inflammation activities. Therefore, the potential of A. argyi and its constituents in reducing the infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was investigated in this study. Results Among the phytochemicals in A. argyi, eriodictyol and umbelliferone were identified to target transmembrane serine protease 2 (TMPRSS2) and angiotensin-converting enzyme 2 (ACE2) proteins, the essential factors for the cellular entry of SARS-CoV-2, in both FRET-based enzymatic assays and molecular docking analyses. These two ingredients of A. argyi suppressed the infection of ACE2-expressed HEK-293 T cells with lentiviral-based pseudo-particles (Vpp) expressing wild-type and variants of SARS-CoV-2 spike (S) protein (SARS-CoV-2 S-Vpp) via interrupting the interaction between S protein and cellular receptor ACE2 and reducing the expressions of ACE2 and TMPRSS2. Oral administration with umbelliferone efficiently prevented the SARS-CoV-2 S-Vpp-induced inflammation in the lung tissues of BALB/c mice. Conclusions Eriodictyol and umbelliferone, the phytochemicals of Artemisia argyi, potentially suppress the cellular entry of SARS-CoV-2 by preventing the protein binding activity of the S protein to ACE2.
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- 2023
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7. Effects of Citrus depressa Hayata juice on high-fat diet-induced obesity in HBV transgenic mice
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Pei-Yi Chu, Chang-Lu Hsu, Yen-An Lin, Yi-Cheng Pan, Yun-Hao Dai, Ying-Chun Yu, Juan-Cheng Yang, Wen-Lung Ma, Yi-Jinn Lillian Chen, Chia-Lin Lee, and Yang-Chang Wu
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Citrus depressa hayata ,Juice ,fermentation ,Polyphenol ,Hesperidin ,High-fat diet-induced obesity ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
The present study investigated the potential anti-obesity properties of Citrus depressa Hayata (CDH) juice in HBV transgenic mice, as well as the impact of fermentation on the effectiveness of the juice. The results revealed that fermentation increased the levels of polyphenols and hesperidin in CDH juice. The animal study demonstrated that both juices were effective in mitigating the weight gain induced by a high-fat diet by correcting metabolic parameter imbalances, reducing hepatic lipid accumulation, and reversing hepatic immune suppression. Furthermore, fermented juice exhibited superior efficacy in managing body weight and inhibiting the expansion of white adipose tissue (WAT). Fermented juice significantly enhanced adiponectin production and PPARγ expression in WAT, while also reducing hypertrophy. This study offers valuable insights into the potential role of CDH juices in combating obesity associated with high fat consumption and underscores the promise of CDH juice as a functional beverage.
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- 2024
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8. Linoleate-pazopanib conjugation as active pharmacological ingredient to abolish hepatocellular carcinoma growth
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Ke Wang, Pei-Yin Liao, Wei-Chun Chang, Cian-Ru Yang, Yu-Ting Su, Ping-Ching Wu, Yang-Chang Wu, Yao-Ching Hung, Najim Akhtar, Hsueh-Chou Lai, and Wen-Lung Ma
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hepatocellular carcinoma ,LDC ,linoleate ,pazopanib ,LAPC ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Small molecule compounds targeting multiple kinases involved in neoangiogenesis have shown survival benefits in patients with unresectable hepatocellular carcinoma (HCC). Nonetheless, despite the beneficial effects of multikinase inhibitors (MKIs), a lack of boosting adjuvant limits their objective response rate. Lipid conjugates have been used to improve delivery efficacy or pharmaceutical benefits for decades. However, the feasibility of utilizing lipid-drug conjugates (LDCs) in HCC regimens remains untested. In this study, oral feeding of linoleate-fluorescein isothiocyanate conjugates showed that the compound was well distributed in a spontaneous HCC mouse model. Therefore, a rationale design was developed for chemically synthesizing a linoleate-pazopanib conjugate (LAPC). The LAPC showed a significantly improved cytotoxicity compared to the parental drug pazopanib. Pazopanib’s angiogenic suppressing signals were not observed in LAPC-treated HCC cells, potentially suggesting an altered mechanism of action (MOA). In an efficacy trial comparing placebo, oral pazopanib, and LAPC treatments in the hepatitis B virus transgene-related spontaneous HCC mouse model (HBVtg-HCC), the LAPC treatment demonstrated superior tumor ablating capacity in comparison to both placebo and pazopanib treatments, without any discernible systemic toxicity. The LAPC exposure is associated with an apoptosis marker (Terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL]) and an enhanced ferroptosis (glutathione peroxidase 4 [GPX4]) potential in HBVtg-HCC tumors. Therefore, the LAPC showed excellent HCC ablative efficacy with altered MOA. The molecular mechanisms of the LAPC and LDCs for HCC therapeutics are of great academic interest. Further comprehensive preclinical trials (e.g., chemical-manufacture-control, toxicity, distribution, and pharmacokinetics/pharmacodynamics) are expected.
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- 2024
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9. The Efficacy of Lactobacillus delbrueckii ssp. bulgaricus Supplementation in Managing Body Weight and Blood Lipids of People with Overweight: A Randomized Pilot Trial
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Pei-Yi Chu, Ying-Chun Yu, Yi-Cheng Pan, Yun-Hao Dai, Juan-Cheng Yang, Kuo-Chin Huang, and Yang-Chang Wu
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Lactobacillus delbrueckii ssp. bulgaricus ,body weight ,triglyceride ,lipoprotein ,obesity ,Microbiology ,QR1-502 - Abstract
This study aimed to evaluate the efficacy of Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) in improving body weight, obesity-related outcomes, and lipid profiles of overweight people. Thirty-six overweight participants were randomly assigned to either a probiotic or a placebo group. A placebo powder or L. bulgaricus powder (containing 1 × 108 colony-forming unit (CFU) of the probiotic) was administered daily for 12 weeks. Body composition was determined, and blood tests were performed before and after the intervention. L. bulgaricus supplementation under the present condition did not affect the body weight, fat percentage, or body mass index (BMI) of the participants, while it resulted in a notable decrease in blood triglyceride (TG) levels, which corresponded to a lowering of the TG proportion in the composition of large VLDL (L–XXL sized fractions) and HDL (M and L fractions) in the probiotic-treated group. These results suggest that L. bulgaricus supplementation under the current conditions may not be helpful for losing weight, but it has the potential to decrease blood TG levels by modulating TG accumulation in or transport by VLDL/HDL in obese patients. L. bulgaricus supplements may have health-promoting properties in preventing TG-related diseases in overweight people.
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- 2024
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10. The protoplast two-way fusions and fusant characteristics of Antrodia cinnamomea and Cordyceps militaris
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Wei-Kuang Lai, Yang-Chang Wu, Tai-Sheng Yeh, Chun-Ren Hsieh, Yi-Hong Tsai, Chien-Kei Wei, Chi-Ying Li, Ying-Chen Lu, and Fang-Rong Chang
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Medicinal fungi ,Protoplast fusion ,Antrodia cinnamomea ,Cordyceps militaris ,Secondary metabolite ,Nutrition. Foods and food supply ,TX341-641 - Abstract
This study generated two fused protoplasts of Antrodia cinnamomea and Cordyceps militaris in two ways. The protoplasts of A. cinnamomea were inactivated by heat to inactivate biochemical processes and enzymatic activities in the cytoplasm, and the protoplasts of C. militaris were inactivated by UV radiation to invalidate their genome function, then they were fused under optimal conditions to get a fusion rate as (7.42 ± 0.8) × 10-6 fusants/mL; the new fusants were abbreviated as Ac-Cm. On the other hand, when A. cinnamomea and C. militaris were treated with heat and UV oppositely using similar experiments, the fusion rate was (9.70 ± 0.68) × 10-5 fusants/mL, and the new fusants were abbreviated as Cm-Ac. We selected each of two best-growing fused colonies Ac-Cm-1, Ac-Cm-2, Cm-Ac-1, and Cm-Ac-2, together with parental A. cinnamomea and C. militaris, and studied their morphology, growth antagonism tests, and genetic relationships by 18S rRNA sequencing. In comparison with the initial cultures of 4 fusants, the yields of adenosine, biomass, cordycepic acid, cordycepin, total polysaccharide, and total triterpenoids were increased up 1.305−50.1563 times in the optimal medium conditions. For gene stability tests, those of the four fusants and their outputs were stabilized within 10 generations.
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- 2022
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11. Association of combination antiretroviral therapy with risk of neurological diseases in patients with HIV/AIDS in Taiwan: a nested case-control study
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Chen-Hsing Chou, Jian-Shiun Chiou, Mao-Wang Ho, Ni Tien, Te-Mao Li, Mu-Lin Chiu, Fuu-Jen Tsai, Yang-Chang Wu, I-Ching Chou, Hsing-Fang Lu, Ting-Hsu Lin, Chiu-Chu Liao, Shao-Mei Huang, Wen-Miin Liang, and Ying-Ju Lin
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antiretroviral therapy ,neurocognitive impairment ,nested case-control study ,cumulative defined daily dose ,CNS penetration effectiveness score ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Heterogeneous neurocognitive impairment remains an important issue, even in the era of combination antiretroviral therapy (cART), with an incidence ranging from 15% to 65%. Although ART drugs with higher penetration scores to the central nervous system (CNS) show better HIV replication control in the CNS, the association between CNS penetration effectiveness (CPE) scores and neurocognitive impairment remains inconclusive. To explore whether ART exposure is associated with the risk of neurological diseases among patients with HIV/AIDS, this study in Taiwan involved 2,571 patients with neurological diseases and 10,284 matched, randomly selected patients without neurological diseases between 2010 and 2017. A conditional logistic regression model was used in this study. The parameters for ART exposure included ART usage, timing of exposure, cumulative defined daily dose (DDD), adherence, and cumulative CPE score. Incident cases of neurological diseases, including CNS infections, cognitive disorders, vasculopathy, and peripheral neuropathy, were obtained from the National Health Insurance Research Database in Taiwan. Odds ratios (ORs) for the risk of neurological diseases were conducted using a multivariate conditional logistic regression model. Patients with a history of past exposure (OR: 1.68, 95% confidence interval [CI]:1.22–2.32), low cumulative DDDs (< 2,500) (OR: 1.28, 95% CI: 1.15–1.42), low adherence (0 < adherence (ADH) ≤ 0.8) (OR: 1.46, 95% CI: 1.30–1.64), or high cumulative CPE scores (>14) (OR: 1.34, 95% CI: 1.14–1.57) had a high risk of neurological diseases. When stratified by classes of ART drugs, patients with low cumulative DDDs or low adherence had a high risk of neurological diseases, including NRTIs, PIs, NNRTIs, INSTIs, and multi-drug tablets. Subgroup analyses also suggested that patients with low cumulative DDDs or low adherence had a high risk of neurological diseases when they had high cumulative CPE scores. Patients with high cumulative DDDs or medication adherence were protected against neurological diseases only when they had low cumulative CPE scores (≤ 14). Patients may be at risk for neurological diseases when they have low cumulative DDDs, low adherence, or usage with high cumulative CPE scores. Continuous usage and low cumulative CPE scores of ART drugs may benefit neurocognitive health in patients with HIV/AIDS.
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- 2023
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12. Ursolic acid silences CYP19A1/aromatase to suppress gastric cancer growth
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Wen‐Lung Ma, Ning Chang, Yingchun Yu, Yu‐Ting Su, Guan‐Yu Chen, Wei‐Chung Cheng, Yang‐Chang Wu, Ching‐Chia Li, Wei‐Chun Chang, and Juan‐Cheng Yang
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Ar silencer ,CYP19A1/aromatase ,gastric cancer ,Hedyotis diffusa Willd ,ursolic acid ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Introduction Gastric cancer (GCa) is a malignancy with few effective treatments. Ursolic acid (UA), a bioactive triterpenoid enriched in Hedyotis diffusa Willd, known to suppress GCa without identified target. CYP19A1 (cytochrome P450 family 19A1; also known as aromatase, Ar) was correlated to GCa prognosis. Relatedly, Ar silencers, which halt the expression of Ar exhibited anti‐GCa effects in experimental models, are currently being investigated. Method The docking simulation score of UA was compared with Ar inhibitors, e.g., letrozole, exemestane, in Ar protein crystallization. Hedyotis diffusa Willd ethanol extract, UA, or 5‐fluracil were applied onto AGS, SC‐M1, MKN45 GCa cells for cancer inhibition tests. Immunoblot for measuring gene expressions upon drug treatments, or gene knockdown/overexpression. Treatments were also applied in a MKN45 implantation tumor model. A web‐based GCa cohort for Ar expression association with prognosis was performed. Result The ethanol extracts of Hedyotis diffusa Willd, enrich with UA, exhibited cytotoxic activity against GCa cells. Molecular docking simulations with the 3D Ar structure revealed an excellent fitting score for UA. UA increase cytotoxic, and suppressed colony, in addition to its Ar silencing capacity. Moreover, UA synergistically facilitated 5‐FU, (a standard GCa treatment) regimen in vitro. Consistent with those results, adding estradiol did not reverse the cancer‐suppressing effects of UA, which confirmed UA acts as an Ar silencer. Furthermore, UA exhibited tumor‐suppressing index (TSI) score of 90% over a 6‐week treatment term when used for single dosing in xenograft tumor model. In the clinical setting, Ar expression was found to be higher in GCa tumors than normal parental tissue from the TCGA (The Cancer Genome Atlas) cohort, while high Ar expression associated with poor prognosis. Together, the results indicate UA could be used to treat GCa by silencing Ar expression in GCa. Hedyotis diffusa Willd ethanol extract could be an functional food supplements.
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- 2022
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13. Antcin K inhibits VCAM-1-dependent monocyte adhesion in human rheumatoid arthritis synovial fibroblasts
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David Achudhan, Sunny Li-Yun Chang, Shan-Chi Liu, Yen-You Lin, Wei-Chien Huang, Yang-Chang Wu, Chien-Chung Huang, Chun-Hao Tsai, Chih-Yuan Ko, Yueh-Hsiung Kuo, and Chih-Hsin Tang
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rheumatoid arthritis ,cell adhesion molecules ,monocytes ,vcam-1 ,cd11b ,antrodia cinnamomea ,antcin k ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Background: Antcin K, an extract of Antrodia cinnamomea (a medicinal mushroom endemic to Taiwan commonly used in Chinese medicine preparations), inhibits proinflammatory cytokine production and angiogenesis in human rheumatoid arthritis synovial fibroblasts (RASFs), major players in RA disease. Antcin K also inhibits disease activity in mice with collagen-induced arthritis (CIA). Up until now, the effects of Antcin K upon cell adhesion molecules (CAMs) were unknown. Methods: RA and healthy synovial tissue samples (n = 10 in each group) were retrieved from the Gene Expression Omnibus (GEO) database (accession code: GDS5401) to compare CAM and monocyte marker expressions. In addition, synovial tissue samples from six RA patients and six patients undergoing arthroscopy for trauma/joint derangement (healthy controls) were subjected to immunohistochemical (IHC) analysis. mRNA and protein expression levels were analyzed in RASFs using RT-qPCR (Reverse transcription-quantitative polymerase chain reaction) and Western blot. RASFs were incubated with Antcin K and examined for monocyte adherence by fluorescence microscopy. Ankle joint tissue specimens from a CIA mouse model and healthy controls were stained with hematoxylin and eosin (H&E) and Safranin-O/Fast Green to examine histological changes and evidence of bone loss. IHC analysis determined levels of vascular cell adhesion molecule 1 (VCAM-1) and CD11b in CIA ankle tissue and clinical synovial tissue. Results: Levels of VCAM-1 expression were higher in the GEO database specimens and the study’s clinical samples of RA synovial tissue compared with the healthy specimens. Antcin K dose-dependently inhibited VCAM-1 expression and monocyte adhesion in RASFs. Antcin K also significantly inhibited levels of VCAM-1 and monocyte CD11b expression in CIA tissue. These effects appeared to be mediated by MEK1/2-ERK, p38, and AP-1 signaling. Conclusions: Antcin K seems promising for the treatment of RA and deserves further investigations.
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- 2022
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14. Effect of Chinese herbal medicine therapy on risks of all-cause mortality, infections, parasites, and circulatory-related mortality in HIV/AIDS patients with neurological diseases
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Jian-Shiun Chiou, Chen-Hsing Chou, Mao-Wang Ho, Ni Tien, Wen-Miin Liang, Mu-Lin Chiu, Fuu-Jen Tsai, Yang-Chang Wu, I-Ching Chou, Hsing-Fang Lu, Ting-Hsu Lin, Chiu-Chu Liao, Shao-Mei Huang, Te-Mao Li, and Ying-Ju Lin
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HIV/AIDS ,neurological diseases ,mortality ,Chinese herbal medicine ,network analysis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Introduction: Long-term living with human immunodeficiency virus (HIV) and/or antiretroviral therapy (ART) is associated with various adverse effects, including neurocognitive impairment. Heterogeneous neurocognitive impairment remains an important issue, affecting between 15–65% of human immunodeficiency virus infection and acquired immunodeficiency syndrome (HIV/AIDS) patients and resulting in work performance, safety, and health-related outcomes that have a heavy economic burden.Methods: We identified 1,209 HIV/AIDS patients with neurological diseases during 2010–2017. The Kaplan–Meier method, log-rank test, and Cox proportional hazards model were used to analyze 308 CHM users and 901 non-CHM users within this population. Major CHM clusters were determined using association rule mining and network analysis.Results and Discussion: Results showed that CHM users had a 70% lower risk of all-cause mortality (adjusted hazard ratio (aHR) = 0.30, 95% confidence interval (CI):0.16–0.58, p < 0.001) (p = 0.0007, log-rank test). Furthermore, CHM users had an 86% lower risk of infections, parasites, and circulatory-related mortality (aHR = 0.14, 95% confidence interval (CI):0.04–0.46, p = 0.001) (p = 0.0010, log-rank test). Association rule mining and network analysis showed that two CHM clusters were important for patients with neurological diseases. In the first CHM cluster, Huang Qin (HQ; root of Scutellaria baicalensis Georgi), Gan Cao (GC; root of Glycyrrhiza uralensis Fisch.), Huang Lian (HL; root of Coptis chinensis Franch.), Jie Geng (JG; root of Platycodon grandiflorus (Jacq.) A.DC.), and Huang Bai (HB; bark of Phellodendron amurense Rupr.) were identified as important CHMs. Among them, the strongest connection strength was identified between the HL and HQ. In the second CHM cluster, Suan-Zao-Ren-Tang (SZRT) and Ye Jiao Teng (YJT; stem of Polygonum multiflorum Thunb.) were identified as important CHMs with the strongest connection strength. CHMs may thus be effective in treating HIV/AIDS patients with neurological diseases, and future clinical trials are essential for the prevention of neurological dysfunction in the population.
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- 2023
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15. Deletion of Jdp2 enhances Slc7a11 expression in Atoh-1 positive cerebellum granule cell progenitors in vivo
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Chia-Chen Ku, Kenly Wuputra, Kohsuke Kato, Jia-Bin Pan, Chia-Pei Li, Ming-Ho Tsai, Michiya Noguchi, Yukio Nakamura, Chung-Jung Liu, Te-Fu Chan, Ming-Feng Hou, Shigeharu Wakana, Yang-Chang Wu, Chang-Shen Lin, Deng-Chyang Wu, and Kazunari K. Yokoyama
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Antioxidation ,Cerebellum ,Granule cells ,Jun dimerization protein 2 (Jdp2) ,Reactive oxygen species (ROS) ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background The cerebellum is the sensitive region of the brain to developmental abnormalities related to the effects of oxidative stresses. Abnormal cerebellar lobe formation, found in Jun dimerization protein 2 (Jdp2)-knockout (KO) mice, is related to increased antioxidant formation and a reduction in apoptotic cell death in granule cell progenitors (GCPs). Here, we aim that Jdp2 plays a critical role of cerebellar development which is affected by the ROS regulation and redox control. Objective Jdp2-promoter-Cre transgenic mouse displayed a positive signal in the cerebellum, especially within granule cells. Jdp2-KO mice exhibited impaired development of the cerebellum compared with wild-type (WT) mice. The antioxidation controlled gene, such as cystine-glutamate transporter Slc7a11, might be critical to regulate the redox homeostasis and the development of the cerebellum. Methods We generated the Jdp2-promoter-Cre mice and Jdp2-KO mice to examine the levels of Slc7a11, ROS levels and the expressions of antioxidation related genes were examined in the mouse cerebellum using the immunohistochemistry. Results The cerebellum of Jdp2-KO mice displayed expression of the cystine-glutamate transporter Slc7a11, within the internal granule layer at postnatal day 6; in contrast, the WT cerebellum mainly displayed Sla7a11 expression in the external granule layer. Moreover, development of the cerebellar lobes in Jdp2-KO mice was altered compared with WT mice. Expression of Slc7a11, Nrf2, and p21Cip1 was higher in the cerebellum of Jdp2-KO mice than in WT mice. Conclusion Jdp2 is a critical regulator of Slc7a11 transporter during the antioxidation response, which might control the growth, apoptosis, and differentiation of GCPs in the cerebellar lobes. These observations are consistent with our previous study in vitro.
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- 2021
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16. Effect of Chinese Herbal Medicine Therapy on Risks of Overall, Diabetes-Related, and Cardiovascular Diseases-Related Mortalities in Taiwanese Patients With Hereditary Hemolytic Anemias
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Mu-Lin Chiu, Jian-Shiun Chiou, Chao-Jung Chen, Wen-Miin Liang, Fuu-Jen Tsai, Yang-Chang Wu, Ting-Hsu Lin, Chiu-Chu Liao, Shao-Mei Huang, Chen-Hsing Chou, Cheng-Wen Lin, Te-Mao Li, Yu-Lung Hsu, and Ying-Ju Lin
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hereditary hemolytic anemias ,overall mortality ,diabetes-related mortality ,cardiovascular diseases-related mortality ,chinese herbal medicine ,network analysis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Hereditary Hemolytic Anemias (HHAs) are a rare but heterogeneous group of erythrocytic diseases, characterized by intrinsic cellular defects due to inherited genetic mutations. We investigated the efficacy of Chinese herbal medicine (CHM) in reducing the overall, diabetes-related, and cardiovascular diseases (CVDs)-related mortalities among patients with HHAs using a nationwide population database. In total, we identified 33,278 patients with HHAs and included 9,222 non-CHM and 9,222 CHM matched pairs after matching. The Cox proportional hazards model was used to compare the risk of mortality between non-CHM and CHM users. The Kaplan-Meier method and log-rank test were used to compare the cumulative incidence mortality between non-CHM and CHM users. The CHM prescription patterns were presented by the association rules and network analyses, respectively. The CHM prescription patterns were presented by the association rules and network analyses, respectively. CHM users showed significant reduced risks for of overall (adjusted hazard ratio [aHR]: 0.67, 95% confidence interval [CI]: 0.61–0.73, p < 0.001), diabetes-related (aHR: 0.57, 95% CI: 0.40–0.82, p < 0.001), and CVDs-related (aHR: 0.59, 95% CI: 0.49–0.72, p < 0.001) mortalities compared with non-CHM users. Two CHM clusters are frequently used to treat Taiwanese patients with HHAs. Cluster 1 is composed of six CHMs: Bei-Mu (BM; Fritillaria cirrhosa D.Don), Gan-Cao (GC; Glycyrrhiza uralensis Fisch.), Hai-Piao-Xiao (HPX; Endoconcha Sepiae), Jie-Geng (JG; Platycodon grandiflorus (Jacq.) A.DC.), Yu-Xing-Cao (YXC; Houttuynia cordata Thunb.), and Xin-Yi-Qing-Fei-Tang (XYQFT). Cluster 2 is composed of two CHMs, Dang-Gui (DG; Angelica sinensis (Oliv.) Diels) and Huang-Qi (HQi; Astragalus membranaceus (Fisch.) Bunge). Further randomized clinical trials are essential to evaluate the safety and effectiveness of above CHM products and to eliminate potential biases in the current retrospective study.
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- 2022
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17. High-Purity Bioactive Ingredient—3S,3′S-Astaxanthin: A New Preparation from Genetically Modified Kluyveromyces marxianus without Column Chromatography and Gel Filtration
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Wei-Cheng Yuan, Tung-Ying Wu, Pei-Yi Chu, Fang-Rong Chang, and Yang-Chang Wu
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enzyme-assisted extraction ,magnesium perchlorate ,ORAC ,salt-assisted liquid-liquid extraction ,yeast ,Therapeutics. Pharmacology ,RM1-950 - Abstract
A highly efficient methodology for bioactive ingredient 3S,3′S-astaxanthin (3S,3′S-AST) preparation from genetically modified yeast (Kluyveromyces marxianus) with a combination of enzyme-assisted extraction and salt-assisted liquid-liquid extraction (SALLE) was achieved. The highest yield of 3S,3′S-AST indicated that FoodPro® CBL for yeast cell walls hydrolysis could significantly enhance extraction and obtain, with the help of SALLE procedure, quantified 3S,3′S-AST over 99% in purity through cation chelation. In the oxygen radical antioxidant capacity (ORAC) assay, the antioxidant capacity of high-purity 3S,3′S-AST products were 18.3 times higher than that of the original raw material extract. This new combination preparation may replace previous methods and has the potential to be scaled up in the manufacture of high-purity 3S,3′S-AST from low-value bioresources of raw materials to high-value products in the food and/or drug industries with lower cost and simple equipment.
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- 2023
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18. Briavioids E–G, Newly Isolated Briarane-Diterpenoids from a Cultured Octocoral Briareum violaceum
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Thanh Hao Huynh, Chia-Jung Liu, Yi-Hung Liu, Su-Ying Chien, Zhi-Hong Wen, Lee-Shing Fang, Jih-Jung Chen, Yang-Chang Wu, Jui-Hsin Su, and Ping-Jyun Sung
- Subjects
Briareum violaceum ,briarane ,briavioid ,X-ray ,anti-inflammation ,iNOS ,Biology (General) ,QH301-705.5 - Abstract
The chemical screening of a cultured soft coral, Briareum violaceum, led to the isolation of eight natural, briarane-related diterpenoids, including three unreported metabolites, briavioids E–G (1–3), and five known briaranes, briacavatolides B (4) and C (5), briaexcavatin L (6), briaexcavatolide U (7) and briarenol K (8). The structures of briaranes 1–8 were established using spectroscopic methods. The absolute configuration of briavioid A (9), obtained in a previous study, was reported for the first time in this study by a single-crystal X-ray diffraction analysis using a copper radiation source. The anti-inflammatory activity of briaranes 1 and 2 and briaranes 4–8 was evaluated by screening their inhibitory ability against the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells.
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- 2023
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19. Soya-cerebroside inhibits VEGF-facilitated angiogenesis in endothelial progenitor cells
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Hsiang-Ping Lee, Shih-Wei Wang, Yang-Chang Wu, Liang-Wei Lin, Fuu-Jen Tsai, Jai-Sing Yang, Te-Mao Li, and Chih-Hsin Tang
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soya-cerebroside ,endothelial progenitor cells ,angiogenesis ,vegf ,Agriculture (General) ,S1-972 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Vascular endothelial growth factor (VEGF) is well recognized as an essential component of angiogenesis and the increased proliferation and migration of endothelial cells. Bone marrow-derived endothelial progenitor cells (EPCs) are involved in VEGF-induced vessel formation during physiological and pathological states. Soya-cerebroside, an extract from Cordyceps militaris, reduces synovial inflammation and prevents cartilage damage in an osteoarthritis model. However, the role of soya-cerebroside in VEGF-regulated EPC angiogenesis is uncertain. Records from the Oncomine database demonstrate higher levels of VEGF in cancerous tissue compared with normal tissue. This study describes VEGF-induced promotion of EPC-associated angiogenesis in vivo and how the treatment of EPCs with soya-cerebroside inhibited VEGF-facilitated migration and tube formation. The study evidence shows that the c-Src, FAK and Runx2 signalling pathways are involved in the inhibitory effects of soya-cerebroside. This novel agent may therefore be used to inhibit EPC-associated angiogenesis.
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- 2020
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20. Soya-cerebroside reduces interleukin production in human rheumatoid arthritis synovial fibroblasts by inhibiting the ERK, NF-κB and AP-1 signalling pathways
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Hsiang-Ping Lee, Yang-Chang Wu, Bo-Cheng Chen, Shan-Chi Liu, Te-Mao Li, Wei-Chien Huang, Chin-Jung Hsu, and Chih-Hsin Tang
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soya-cerebroside ,rheumatoid arthritis ,il-1β ,il-6 ,il-8 ,Agriculture (General) ,S1-972 ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Rheumatoid arthritis (RA) is characterised by the infiltration of a number of proinflammatory cytokines into synovial fluid. Soya-cerebroside, an extract from Cordyceps militaris, inhibits synovial inflammation and reduces cartilage damage in an osteoarthritis model, although the role of soya-cerebroside in inflammatory cytokine expression in RA is uncertain. In this study, Gene Expression Omnibus (GEO) database records revealed higher levels of interleukin (IL)-1β, IL-6 and IL-8 in RA tissue compared with normal tissue. Elevated levels of these inflammatory cytokines were also higher in mice with collagen-induced arthritis (CIA) than in control mice. Soya-cerebroside effectively inhibited IL-1β, IL-6 and IL-8 expression in RA synovial fibroblasts, apparently by inhibiting the ERK, NF-κB and AP-1 signalling pathways. This anti-inflammatory agent shows promise for the treatment of RA.
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- 2020
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21. Cordycerebroside A suppresses VCAM-dependent monocyte adhesion in osteoarthritis synovial fibroblasts by inhibiting MEK/ERK/AP-1 signaling
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Hsiang-Ping Lee, Shan-Chi Liu, Yu-Han Wang, Bo-Cheng Chen, Hsien-Te Chen, Te-Mao Li, Wei-Chien Huang, Chin-Jung Hsu, Yang-Chang Wu, and Chih-Hsin Tang
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Cordycerebroside A ,Osteoarthritis ,VCAM-1 ,Monocyte adhesion ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Osteoarthritis (OA) is characterized by the infiltration and adhesion of monocyte into the joint synovium. Vascular cell adhesion molecule 1 (VCAM-1) is a critical cell adhesion molecule that controls monocyte motility during OA progression. Cordycerebroside A, a cerebroside compound isolated from Cordyceps militaris, inhibits the production of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in synovial macrophages, but has not yet been investigated in OA. Gene Expression Omnibus (GEO) dataset analysis revealed higher levels of VCAM-1 and CD11b (a monocyte marker) in OA synovial tissue compared with normal healthy tissue. The same results were observed in anterior cruciate ligament transaction (ACLT)-induced OA in rats compared with normal healthy controls. Cordycerebroside A markedly suppressed VCAM-1 expression and monocyte adhesion in human OA synovial fibroblasts. The MEK, ERK and AP-1 signaling cascades regulated cordycerebroside A-induced inhibition of VCAM-1 production. Thus, cordycerebroside A is a promising agent for the treatment of OA.
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- 2021
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22. Betulin suppresses TNF-α and IL-1β production in osteoarthritis synovial fibroblasts by inhibiting the MEK/ERK/NF-κB pathway
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Chin-Horng Su, Chih-Yang Lin, Chun-Hao Tsai, Hsiang-Ping Lee, Lun-Chien Lo, Wei-Chien Huang, Yang-Chang Wu, Ching-Liang Hsieh, and Chih-Hsin Tang
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Betulin ,Osteoarthritis ,TNF-α ,IL-1β ,ACLT ,Proinflammatory cytokines ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Osteoarthritis (OA) is characterized by the infiltration of a number of proinflammatory cytokines into the joint microenvironment. Betulin is a natural analogue of betulinic acid, a lupane-type pentacyclic triterpene that displays many important biological activities, including anti-inflammatory activity, although the effects on inflammatory cytokine production in OA are unknown. Analyses of samples from the Gene Expression Omnibus (GEO) dataset and our own clinical samples revealed higher levels of tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β) in OA synovial tissue compared with normal healthy tissue. This was also the case in synovial tissue from normal healthy rats and rats subjected to anterior cruciate ligament transaction (ACLT)-induced OA. High-throughput screening of inflammatory cytokines in betulin-treated OA synovial fibroblasts (OASFs) identified TNF-α and IL-1β as potential targets of betulin. Further analyses found that betulin suppresses TNF-α and IL-1β production in OASFs by inhibiting mitogen-activated protein kinase (MEK), extracellular signal-regulated kinase (ERK) and nuclear factor kappa B (NF-κB) signaling cascades. Betulin shows promise for the treatment of OA.
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- 2021
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23. Effect of Xanthium Strumarium on HIV-1 5′-LTR Transcriptional Activity and Viral Reactivation in Latently Infected Cells
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Chao-Jung Chen, Mu-Lin Chiu, Chien-Hui Hung, Wen-Miin Liang, Mao-Wang Ho, Ting-Hsu Lin, Xiang Liu, Hsinyi Tsang, Chiu-Chu Liao, Shao-Mei Huang, Yi-Fang Wu, Yang-Chang Wu, Te-Mao Li, Fuu-Jen Tsai, and Ying-Ju Lin
- Subjects
human immunodeficiency virus type 1 latency ,Chinese herbal medicine ,X. strumarium ,5′-long terminal repeat ,nuclear regulatory proteins ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Chinese herbal medicines (CHMs) are widely used in Asian countries. They show multiple pharmacological activities, including antiviral activities. The 5′-long terminal repeat (LTR) region of HIV-1, required for viral transcription, is a potential drug target for HIV-1 reactivation and intrinsic cell death induction of infected or latently infected cells. Modulation of HIV-1 reactivation requires interactions between host cell proteins and viral 5′-LTR elements. By evaluation of two CHMs- Xanthium strumarium and Pueraria montana, we found that 1) X. strumarium reactivated HIV-1 latently infected cells in J-Lat 8.4, J-Lat 9.2, U1, and ACH-2 cells in vitro; 2) 27 nuclear regulatory proteins were associated with HIV-1 5′-LTR using deoxyribonucleic acid affinity pull-down and LC-MS/MS analyses; and 3) among them, silencing of XRCC6 reactivated HIV-1 5′-LTR transcriptional activity. We found that X. strumarium inhibits the 5′-LTR associated XRCC6 nuclear regulatory proteins, increases its viral 5′-LTR promoter transcriptional activity, and reactivates HIV-1 latently infected cells in vitro. These findings may contribute to understanding the 5′-LTR activity and the host cell nuclear regulatory protein machinery for reactivating HIV-1 and for future investigations to eradicate and cure HIV-1 infection.
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- 2021
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24. The Amiloride Derivatives Regulate the Alternative Splicing of Apoptotic Gene Transcripts.
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Chien-Chih Lee, Wen-Hsin Chang, Ting-Yuan Liu, Yu-Chia Chen, Guan-Yu Chen, Yang-Chang Wu, and Jan-Gowth Chang
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- 2018
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25. Chemical Composition and Chronic Toxicity of Disc-Cultured Antrodia cinnamomea Fruiting Bodies
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Shou-Chou Liu, Tung-Ying Wu, Tai-Hao Hsu, Ming-Nan Lai, Yang-Chang Wu, and Lean-Teik Ng
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Antrodia cinnamomea ,disc culture ,fruiting bodies ,bioactive triterpenoids ,toxicological properties ,Chemical technology ,TP1-1185 - Abstract
Antrodia cinnamomea (AC) is a popular fungus for use as folk medicine in health maintenance and disease prevention and treatment. Disc culture is a novel technique for producing AC fruiting bodies. This study aimed to investigate the bioactive components and toxicological properties of disc-cultured AC fruiting body powders (ACP) in rats. The HPLC technique was used to quantify the composition of bioactive triterpenoids in ACP. Toxicological properties were evaluated on male and female Sprague-Dawley rats receiving ACP orally at 200, 600, and 1000 mg/kg body weight for 90 days; the control group received only distilled water. The results show that ACP contained seven important AC index compounds, namely antcins A, B, C, K, and H, dehydrosulphurenic acid, and dehydroeburicoic acid. At the tested doses, oral ACP administration for 90 days caused no mortality, adverse effects on general health, body and organ weights, and food intake. Furthermore, no significant variations were observed in hematological and biochemical parameters among either sex of ACP-treated and control animals. An histopathological examination of vital organs showed no significant structural changes in organs, even in high-dose ACP-treated animals. This study indicated that ACP contained the major bioactive triterpenoids of AC fruiting bodies, and its no-observed-adverse-effect level (NOAEL) was 1000 mg/kg/day, about 20 times the recommended daily intake.
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- 2022
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26. Novel Aporphine- and Proaporphine–Clerodane Hybrids Identified from the Barks of Taiwanese Polyalthia longifolia (Sonn.) Thwaites var. pendula with Strong Anti-DENV2 Activity
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I-Wen Lo, Geng-You Liao, Jin-Ching Lee, Chi-I Chang, Yang-Chang Wu, Yen-Yu Chen, Shang-Pin Liu, Huey-Jen Su, Chih-I Liu, Chia-Yi Kuo, Zheng-Yu Lin, Tsung-Lin Li, Yun-Sheng Lin, and Chia-Ching Liaw
- Subjects
Polyalthia longifolia var. pendula ,clerodane ,aporphine ,proaporphine ,anti-DENV2 ,NS2B-NS3 protease ,Medicine ,Pharmacy and materia medica ,RS1-441 - Abstract
Hybrid natural products produced via mixed biosynthetic pathways are unique and often surprise one with unexpected medicinal properties in addition to their fascinating structural complexity/diversity. In view of chemical structures, hybridization is a way of diversifying natural products usually through dimerization of two similar or dissimilar subcomponents through a C–C or N–C covalent linkage. Here, we report four structurally attractive diterpene–alkaloid conjugates polyalongarins A–D (1–4), clerodane-containing aporphine and proaporphine alkaloids, the first of its kind from the barks of Taiwanese Polyalthia longifolia (Sonn.) Thwaites var. pendula. In addition to conventional spectroscopic analysis, single crystal X-ray crystallography was employed to determine the chemical structures and stereo-configurations of 1. Compounds 1–4 were subsequently subjected to in vitro antiviral examination against DENV2 by evaluating the expression level of the NS2B protein in DENV2-infected Huh-7 cells. These compounds display encouraging anti-DENV2 activity with superb EC50 (2.8–6.4 μM) and CC50 values (50.4–200 μM). The inhibitory mechanism of 1–4 on NS2B was further explored drawing on in-silico molecular docking analysis. Based on calculated binding affinities and predicted interactions between the functional groups of 1–4 and the allosteric-site residues of the DENV2 NS2B-NS3 protease, our analysis concludes that the clerodane–aporphine/proaporphine-type hybrids are novel and effective DENV NS2B-NS3 protease inhibitors.
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- 2022
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27. Targeting PSEN1 by lnc-CYP3A43-2/miR-29b-2-5p to Reduce β Amyloid Plaque Formation and Improve Cognition Function
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Wei Wuli, Shinn-Zong Lin, Shee-Ping Chen, Bakhos A. Tannous, Wen-Sheng Huang, Peng Yeong Woon, Yang-Chang Wu, Hsueh-Hui Yang, Yi-Cheng Chen, Renata Lopes Fleming, Jack T. Rogers, Catherine M. Cahill, Tsung-Jung Ho, Tzyy-Wen Chiou, and Horng-Jyh Harn
- Subjects
n-butylidenephthalide ,Alzheimer’s disease ,miR-29b-2-5p ,Presenilin1 ,β-amyloid plaque ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Presenilin-1 (PSEN1) is a crucial subunit within the γ-secretase complex and regulates β-amyloid (Aβ) production. Accumulated evidence indicates that n-butylidenephthalide (BP) acts effectively to reduce Aβ levels in neuronal cells that are derived from trisomy 21 (Ts21) induced pluripotent stem cells (iPSCs). However, the mechanism underlying this effect remains unclear. This article aims to investigate the possible mechanisms through which BP ameliorates the development of Alzheimer’s disease (AD) and verify the effectiveness of BP through animal experiments. Results from RNA microarray analysis showed that BP treatment in Ts21 iPSC-derived neuronal cells reduced long noncoding RNA (lncRNA) CYP3A43-2 levels and increased microRNA (miR)-29b-2-5p levels. Bioinformatics tool prediction analysis, biotin-labeled miR-29b-2-5p pull-down assay, and dual-luciferase reporter assay confirmed a direct negative regulatory effect for miRNA29b-2-5p on lnc-RNA-CYP3A43-2 and PSEN1. Moreover, BP administration improved short-term memory and significantly reduced Aβ accumulation in the hippocampus and cortex of 3xTg-AD mice but failed in miR-29b-2-5p mutant mice generated by CRISP/Cas9 technology. In addition, analysis of brain samples from patients with AD showed a decrease in microRNA-29b-2-5p expression in the frontal cortex region. Our results provide evidence that the LncCYP3A43-2/miR29-2-5p/PSEN1 network might be involved in the molecular mechanisms underlying BP-induced Aβ reduction.
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- 2022
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28. Chinese Herbal Medicine Usage Reduces Overall Mortality in HIV-Infected Patients With Osteoporosis or Fractures
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Mao-Wang Ho, Te-Mao Li, Ju-Pi Li, Jian-Shiun Chiou, Mu-Lin Chiu, Chao-Jung Chen, Chi-Fung Cheng, Fuu-Jen Tsai, Yang-Chang Wu, Ting-Hsu Lin, Chiu-Chu Liao, Shao-Mei Huang, Yu-Ning Lin, Chen-Hsing Chou, Wen-Miin Liang, and Ying-Ju Lin
- Subjects
HIV ,osteoporosis ,fracture ,overall mortality ,Chinese herbal medicine ,network analysis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The survival of patients with HIV has greatly improved, due to Anti-Retroviral Therapy (ART). However, long-term HIV survivors often develop serious bone abnormalities, possibly due to the interplay of osteoblasts, osteoclasts, HIV ad ART. We evaluated in a nation-wide study in Taiwan the effect of Chinese herbal medicine (CHM) on overall mortality in HIV patients with osteoporosis or fractures. Enrollment period was between 1998 and 2011. Patients with osteoporosis or fractures before the HIV infection, and those with less than 14 days CHM use, were excluded. This left 498 patients, 160 CHM users, 338 without CHM. Univariate Kaplan-Meier and multivariate Cox regression analysis were used to compare the overall mortality in these 2 groups. Due to the nature of Chinese medicine, CHMs inevitably varied. We therefore also used rule mining and network analysis to determine which major CHM clusters were prescribed to the patients. CHM users had a much Lower mortality (hazard ratio (HR) = 0.43, 95% confidence interval (CI): 0.24–0.77, p < 0.005) and higher survival (p = 0.004, log-rank test). Although the CHMs greatly varied, network analysis identified one main cluster of strongly related CHM combinations (Chuan-Xiong-Cha-Tiao-San (CXCTS), Gan-Cao (GC; Glycyrrhiza uralensis Fisch.), Liu-He-Tang (LHT), Huang-Qin-Tang (HQT), Jia-Wei-Ping-Wei-San (JWPWS), and Dang-Gui-Long-Hui-Wan (DGLHuiW)). CHM as an additional treatment strongly improves overall survival in HIV-infected patients with osteoporosis and fractures.
- Published
- 2021
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29. Uncovering Modern Clinical Applications of Fuzi and Fuzi-Based Formulas: A Nationwide Descriptive Study With Market Basket Analysis
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Chi-Jung Tai, Mohamed El-Shazly, Yi-Hong Tsai, Dezső Csupor, Judit Hohmann, Yang-Chang Wu, Tzyy-Guey Tseng, Fang-Rong Chang, and Hui-Chun Wang
- Subjects
aconitum ,clinical epidemiology ,data mining ,Fuzi ,market basket analyses ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: As time evolved, traditional Chinese medicine (TCM) became integrated into the global medical system as complementary treatments. Some essential TCM herbs started to play a limited role in clinical practices because of Western medication development. For example, Fuzi (Aconiti Lateralis Radix Praeparata) is a toxic but indispensable TCM herb. Fuzi was mainly used in poor circulation and life-threatening conditions by history records. However, with various Western medication options for treating critical conditions currently, how is Fuzi used clinically and its indications in modern TCM are unclear. This study aimed to evaluate Fuzi and Fuzi-based formulas in modern clinical practices using artificial intelligence and data mining methods.Methods: This nationwide descriptive study with market basket analysis used a cohort selected from the Taiwan National Health Insurance database that contained one million national representatives between 2003 and 2010 used for our analysis. Descriptive statistics were performed to demonstrate the modern clinical indications of Fuzi. Market basket analysis was calculated by the Apriori algorithm to discover the association rules between Fuzi and other TCM herbs.Results: A total of 104,281 patients using 405,837 prescriptions of Fuzi and Fuzi-based formulas were identified. TCM doctors were found to use Fuzi in pulmonary (21.5%), gastrointestinal (17.3%), and rheumatologic (11.0%) diseases, but not commonly in cardiovascular diseases (7.4%). Long-term users of Fuzi and Fuzi-based formulas often had the following comorbidities diagnosed by Western doctors: osteoarthritis (31.0%), peptic ulcers (29.5%), hypertension (19.9%), and COPD (19.7%). Patients also used concurrent medications such as H2-receptor antagonists, nonsteroidal anti-inflammatory drugs, β-blockers, calcium channel blockers, and aspirin. Through market basket analysis, for the first time, we noticed many practical Fuzi-related herbal pairs such as Fuzi–Hsihsin (Asari Radix et Rhizoma)–Dahuang (Rhei Radix et Rhizoma) for neurologic diseases and headache.Conclusion: For the first time, big data analysis was applied to uncover the modern clinical indications of Fuzi in addition to traditional use. We provided necessary evidence on the scientific use of Fuzi in current TCM practices, and the Fuzi-related herbal pairs discovered in this study are helpful to the development of new botanical drugs.
- Published
- 2021
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30. Timing, Dosage, and Adherence of Antiretroviral Therapy and Risk of Osteoporosis in Patients With Human Immunodeficiency Virus Infection in Taiwan: A Nested Case-Control Study
- Author
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Mu-Lin Chiu, Wen-Miin Liang, Ju-Pi Li, Chi-Fung Cheng, Jian-Shiun Chiou, Mao-Wang Ho, Yang-Chang Wu, Ting-Hsu Lin, Chiu-Chu Liao, Shao-Mei Huang, Fuu-Jen Tsai, and Ying-Ju Lin
- Subjects
HIV ,osteoporosis ,antiretroviral therapy ,usage timing ,dosage ,adherence ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The progression of acquired immunodeficiency syndrome is delayed in patients with human immunodeficiency virus (HIV) infection receiving antiretroviral therapy (ART). However, long-term ART is associated with adverse effects. Osteoporosis is one of the adverse effects and is a multifactorial systemic skeletal disease associated with bone fragility and an increased risk of fracture. We performed a longitudinal, comprehensive, nested case-control study to explore the effect of ART on the risk of osteoporosis in 104 osteoporotic and 416 non-osteoporotic patients with HIV infection at their average age about 29 years old in Taiwan. Patients with history of ART, current exposure to ART, higher cumulative defined daily doses (DDDs), or higher ART adherence were at a higher risk of osteoporosis (p < 0.05). Patients receiving nucleoside/nucleotide reverse transcriptase inhibitor (NRTI)-containing regimen (zidovudine-lamivudine combination, lamivudine-abacavir combination, and abacavir alone) and protease inhibitor (PI)-containing regimen (lopinavir-ritonavir combination, ritonavir, and atazanavir) had a higher risk of osteoporosis (p < 0.05). Especially, patients receiving high doses of the PIs lopinavir-ritonavir combination had an increased risk of osteoporosis (p < 0.05). In conclusion, history of ART, current exposure to ART, higher cumulative DDDs, and higher ART adherence were associated with an increased risk of osteoporosis. Furthermore, NRTI- and PI-containing regimens and high doses of PIs lopinavir-ritonavir combination may be associated with an increased risk of osteoporosis in patients with HIV infection in Taiwan.
- Published
- 2021
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31. 19-(Benzyloxy)-19-oxojolkinolide B (19-BJB), an ent-abietane diterpene diepoxide, inhibits the growth of bladder cancer T24 cells through DNA damage.
- Author
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Ke Wang, Juan-Cheng Yang, Yeong-Jiunn Jang, Guan-Yu Chen, Ya-Jing Zhang, Yun-Hao Dai, Da-Yong Zhang, and Yang-Chang Wu
- Subjects
Medicine ,Science - Abstract
Diterpenoids jolkinolide A and B, were first isolated from Euphorbia fischeriana. In our previous research, 19-(Benzyloxy)-19-oxojolkinolide B (19-BJB), a derivative of jolkinolides, was synthesized as a novel ent -abietane diterpene diepoxide. In this study, 19-BJB showed strong in vitro activity against bladder cancer cell lines. DNA damage which was observed through the interaction of 19-BJB with nucleotide chains and affected DNA repair resulted in the activation of checkpoint kinase 1 (Chk1) and checkpoint kinase 2 (Chk2) in bladder cancer cell lines. In vivo testing in nude mice also proved that 19-BJB revealed a potential inhibitory effect on tumor growth. Additionally, the 3D-QSAR models of jolkinolides were established. Briefly, we proved that 19-BJB could potentially be used as a drug to inhibit the growth of bladder tumor.
- Published
- 2021
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32. Complementary Chinese Herbal Medicine Therapy Improves Survival in Patients With Pemphigus: A Retrospective Study From a Taiwan-Based Registry
- Author
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Po-Yuan Wu, Te-Mao Li, Shu-I. Chen, Chao-Jung Chen, Jian-Shiun Chiou, Ming-Kuem Lin, Fuu-Jen Tsai, Yang-Chang Wu, Ting-Hsu Lin, Chiu-Chu Liao, Shao-Mei Huang, Yu-Ning Lin, Wen-Miin Liang, and Ying-Ju Lin
- Subjects
pemphigus ,overall mortality ,Chinese herbal medicine ,association rule mining ,network ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Pemphigus is a life-threatening and skin-specific inflammatory autoimmune disease, characterized by intraepidermal blistering between the mucous membranes and skin. Chinese herbal medicine (CHM) has been used as an adjunct therapy for treating many diseases, including pemphigus. However, there are still limited studies in effects of CHM treatment in pemphigus, especially in Taiwan. To more comprehensively explore the effect of long-term CHM treatment on the overall mortality of pemphigus patients, we performed a retrospective analysis of 1,037 pemphigus patients identified from the Registry for Catastrophic Illness Patients database in Taiwan. Among them, 229 and 177 patients were defined as CHM users and non-users, respectively. CHM users were young, predominantly female, and had a lesser Charlson comorbidity index (CCI) than non-CHM users. After adjusting for age, sex, prednisolone use, and CCI, CHM users had a lower overall mortality risk than non-CHM users (multivariate model: hazard ratio (HR): 0.422, 95% confidence interval (CI): 0.242–0.735, p = 0.0023). The cumulative incidence of overall survival was significantly higher in CHM users than in non-users (p = 0.0025, log rank test). Association rule mining and network analysis showed that there was one main CHM cluster with Qi–Ju–Di–Huang–Wan (QJDHW), Dan–Shen (DanS; Radix Salviae miltiorrhizae; Salvia miltiorrhiza Bunge), Jia–Wei–Xiao–Yao-–San (JWXYS), Huang–Lian (HL; Rhizoma coptidis; Coptis chinensis Franch.), and Di–Gu–Pi (DGP; Cortex lycii; Lycium barbarum L.), while the second CHM cluster included Jin–Yin–Hua (JYH; Flos lonicerae; Lonicera hypoglauca Miq.) and Lian–Qiao (LQ; Fructus forsythiae; Forsythia suspensa (Thunb.) Vahl). In Taiwan, CHMs used as an adjunctive therapy reduced the overall mortality to approximately 20% among pemphigus patients after a follow-up of more than 6 years. A comprehensive CHM list may be useful in future clinical trials and further scientific investigations to improve the overall survival in these patients.
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- 2020
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- View/download PDF
33. Composition decipherment of Ficus pumila var. awkeotsang and its potential on COVID-19 symptom amelioration and in silico prediction of SARS-CoV-2 interference
- Author
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Hao-Chun Hu, Szu-Yin Yu, Xiao-Shan Hung, Chun-Han Su, Yu-Liang Yang, Chien-Kei Wei, Yuan-Bin Cheng, Yang-Chang Wu, Chia-Hung Yen, Tsong-Long Hwang, Shu-Li Chen, István Szatmári, Attila Hunyadi, Yi-Hong Tsai, and Fang-Rong Chang
- Subjects
Pharmacology ,03.01. Általános orvostudomány ,Food Science - Published
- 2022
34. Natural Products from Octocorals of the Genus Verrucella (Milne Edwards & Haime, 1857)
- Author
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Ping-Jyun Sung, Chia-Ching Liaw, Yu-Jen Wu, Choo-Aun Neoh, Yu-Chi Tsai, Yi-Hao Lo, Yang-Chang Wu, and Zhi-Hong Wen
- Subjects
Organic Chemistry - Abstract
Abstract: In this review, we summarize the diverse marine natural compounds obtained from the genus Verrucella and their pharmacological effects and present a survey of the natural products taxonomy according to species. Sixty-six natural substances, including fifty-three steroids, six briarane-type diterpenoids, five nitrogenous bases, one amide, and one glycerol derivative, were obtained from Verrucella spp., which were mainly collected from the South China Sea and the Bay of Bengal, India. Steroids were the major constituents found in Verrucella spp. Moreover, anticancer, antimicrobial, and immunomodulatory activities of those steroids and other compounds are also presented. This review reveals the chemical diversity, chemotaxonomy, and multiple pharmacological effects of the Verrucella genus. It will be helpful for further research and exploration of new steroid analogs and other components with prospective biological activities from this marine organism.
- Published
- 2022
35. Steroidal alkaloids from Solanum erianthum and their anti-breast cancer properties
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Chia-Lin Lee, Wei-Yu Hsu, Chao-Jung Chen, and Yang-Chang Wu
- Subjects
Plant Science ,Agronomy and Crop Science ,Biochemistry ,Biotechnology - Published
- 2022
36. Hesperidin Is a Potential Inhibitor against SARS-CoV-2 Infection
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Fang-Ju Cheng, Thanh-Kieu Huynh, Chia-Shin Yang, Dai-Wei Hu, Yi-Cheng Shen, Chih-Yen Tu, Yang-Chang Wu, Chih-Hsin Tang, Wei-Chien Huang, Yeh Chen, and Chien-Yi Ho
- Subjects
hesperidin ,TMPRSS2 ,ACE2 ,SARS-CoV-2 ,D614G ,501Y.v2 ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Hesperidin (HD) is a common flavanone glycoside isolated from citrus fruits and possesses great potential for cardiovascular protection. Hesperetin (HT) is an aglycone metabolite of HD with high bioavailability. Through the docking simulation, HD and HT have shown their potential to bind to two cellular proteins: transmembrane serine protease 2 (TMPRSS2) and angiotensin-converting enzyme 2 (ACE2), which are required for the cellular entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Our results further found that HT and HD suppressed the infection of VeroE6 cells using lentiviral-based pseudo-particles with wild types and variants of SARS-CoV-2 with spike (S) proteins, by blocking the interaction between the S protein and cellular receptor ACE2 and reducing ACE2 and TMPRSS2 expression. In summary, hesperidin is a potential TMPRSS2 inhibitor for the reduction of the SARS-CoV-2 infection.
- Published
- 2021
- Full Text
- View/download PDF
37. Synthesis of Novel Spiro-Tetrahydroquinoline Derivatives and Evaluation of Their Pharmacological Effects on Wound Healing
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Yan-Cheng Liou, Yan-An Lin, Ke Wang, Juan-Cheng Yang, Yeong-Jiunn Jang, Wenwei Lin, and Yang-Chang Wu
- Subjects
tetrahydroquinoline ,1,3-indandione ,spiro-tetrahydroquinoline ,one-pot reaction ,wound healing ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
A highly diastereoselective method for the synthesis of novel spiro-tetrahydroquinoline derivatives is reported here, using a one-pot reaction method. All compounds were characterized by 1H-nuclear magnetic resonance (NMR) and mass spectroscopy, and their stereo configurations were confirmed by X-ray analysis. These activities of these derivatives were then tested in human keratocyte cells. The responses of cells to treatment with selected compounds were studied using scratch analysis, and the compounds were tested in a mouse excision wound model. Three of the derivatives demonstrated significant wound-healing activities.
- Published
- 2021
- Full Text
- View/download PDF
38. Research and development of Cordyceps in Taiwan
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Ching-Peng Chiu, Tsong-Long Hwang, You Chan, Mohamed El-Shazly, Tung-Ying Wu, I-Wen Lo, Yu-Ming Hsu, Kuei-Hung Lai, Ming-Feng Hou, Shyng-Shiou Yuan, Fang-Rong Chang, and Yang-Chang Wu
- Subjects
Entomopathogenic fungi ,Biofunction ,Cordyceps sp. ,C. sinensis ,C. militaris ,C. cicadae ,C. sobolifera ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Cordyceps is treasured entomopathogenic fungi that have been used as antitumor, immunomodulating, antioxidant, and pro-sexual agent. Cordyceps, also called DongChongXiaCao in Chinese, Yartsa Gunbu (Tibetan), means winter worm-summer grass. Natural Cordyceps sinensis with parasitic hosts is difficult to be collected and the recent findings on its potential pharmacological functions, resulted in skyrocketing prices. Therefore, finding a mass-production method or an alternative for C. sinensis products is a top-priority task. In this review, we describe current status of Cordyceps research and its recent developments in Taiwan. The content and pharmacological activities of four major industrial species of Cordyceps (C. sinensis, Cordyceps militaris, Cordyceps cicadae and Cordyceps sobolifera) used in Taiwan, were reviewed. Moreover, we highlighted the effect of using different methods of fermentation and production on the morphology and chemical content of Cordyceps sp. Finally, we summarized the bottle-necks and challenges facing Cordyceps research as well as we proposed future road map for Cordyceps industry in Taiwan.
- Published
- 2016
- Full Text
- View/download PDF
39. Supplementary Figures 1 - 8 from Inhibition of ATR-Dependent Signaling by Protoapigenone and Its Derivative Sensitizes Cancer Cells to Interstrand Cross-link–Generating Agents In Vitro and In Vivo
- Author
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Yang-Chang Wu, Attila Hunyadi, Da-Wei Chuang, Fang-Rong Chang, Wen-Lien Lin, Kevin Yen-Ting Liu, Chao-Neng Tseng, Chin-Chung Wu, Wen-Cheng Chou, Alan Yueh-Luen Lee, and Hui-Chun Wang
- Abstract
PDF file, 984KB.
- Published
- 2023
40. Supplementary Methods from Inhibition of ATR-Dependent Signaling by Protoapigenone and Its Derivative Sensitizes Cancer Cells to Interstrand Cross-link–Generating Agents In Vitro and In Vivo
- Author
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Yang-Chang Wu, Attila Hunyadi, Da-Wei Chuang, Fang-Rong Chang, Wen-Lien Lin, Kevin Yen-Ting Liu, Chao-Neng Tseng, Chin-Chung Wu, Wen-Cheng Chou, Alan Yueh-Luen Lee, and Hui-Chun Wang
- Abstract
PDF file, 75KB.
- Published
- 2023
41. Chemo‐ and Regioselective Construction of Functionalized Isocoumarin, Flavone, and Isoquinolinedione via a One‐pot Reaction of o ‐Quinol Acetate and Soft Nucleophiles
- Author
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Yeong‐Jiunn Jang, Guan‐Yu Chen, Yun‐Lian Jhan, Pei‐Ting Lo, Wei‐Yu Hsu, Ke Wang, Ya‐Ting Hsu, Chia‐Lin Lee, Yu‐Liang Yang, and Yang‐Chang Wu
- Subjects
General Chemistry - Published
- 2023
42. Regiodivergent Synthesis of Methylene and Methyl Ring-Fused Isoquinolinones: Base-Promoted Isomerization of N-Allyl Amides
- Author
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Li-Yuan Chen, Rong-Shiow Tang, Yang-Chang Wu, Chin-Hung Lai, and Ta-Hsien Chuang
- Subjects
Organic Chemistry - Published
- 2022
43. Snail Mucus Enhances Chemosensitivity of Triple-negative Breast Cancer Via Activation of the Fas Pathway
- Author
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CHIEN-YI HO, DAI-WEI HU, BO-RONG CHEN, CHUN-CHUN YANG, CHUN-HSU YAO, TIEN NI, WEI-CHUAN HO, YUEH-SHENG CHEN, CHIH-YEN TU, WEI-CHAO CHANG, YANG-CHANG WU, CHIH-HSIN TANG, HUI-CHI HUANG, WEI-CHIEN HUANG, and TZONG-SHIUN LI
- Subjects
Cancer Research ,Oncology ,General Medicine - Published
- 2022
44. Artemisia argyi potentially prevents the infections with SARS-CoV-2 variants
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Fang-Ju Cheng, Thanh Kieu Huynh, Hung-Yu Ko, Chen-Si Hsueh, Yi-Lun Yeh, Yang-Chang Wu, Chih-Hsin Tang, Chia-Hung Chen, Chih-Yen Tu, Wei-Chien Huang, and Chien-Yi Ho
- Abstract
Background Traditional Chinese medicine (TCM) has potential benefits to prevent multi-viral infection including by modulating the immune system or defending oxidative stress. Artemisia argyi (A. argyi) has been widely used for anti-microbial infection, anti-allergy, anti-diabetes, and anti-inflammation in Eastern Asia. However, it remain unclear whether A. argyi has the potential to reduce the infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Results Through the docking simulation, eriodictyol and umbelliferone, two phytochemicals existed in Artemisia argyi, have showed their potential to bind to cellular proteins transmembrane serine protease 2 (TMPRSS2) and angiotensin-converting enzyme 2 (ACE2), which are required for the cellular entry of SARS-CoV-2. Our results further found that eriodictyol and umbelliferone suppressed the infection of ACE2-expressed HEK-293T cells with lentiviral-based pseudo-particles expressing wild type and variants of SARS-CoV-2 spike (S) protein via interrupting the interaction between S protein and cellular receptor ACE2 and via reducing ACE2 and TMPRSS2 expressions. Conclusions In summary, Artemisia argyi and its ingredients eriodictyol and umbelliferone are potential agents to reduce SARS-CoV-2 infection.
- Published
- 2023
45. Theoretical study of cyano‐promoted intramolecular aza‐Diels–Alder reaction
- Author
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Chin‐Hung Lai, Cheng‐En Yang, Yang‐Chang Wu, Youness El Bakri, Eduardo Chamorro, and Ta‐Hsien Chuang
- Subjects
Organic Chemistry ,Physical and Theoretical Chemistry - Published
- 2022
46. Using NMR, X-ray, and CD analysis in the study on natural products obtained from Vietnamese plant and fungi in terms of pharmaceutical product development
- Author
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Dinh Thang Tran, Cong Dung Vo, Ngoc Tuan Nguyen, Manh Dung Doan, Yang-Chang Wu, and Tian-Shung Wu
- Subjects
absolute configuration ,circular dichroism ,NMR ,X-ray analysis ,Science - Abstract
NMR, X-ray analysis, and CD methods are powerful techniques for the study of absolute configuration of bioactive compounds from natural resources. This study presents the results of a joint-study between Vietnam and Taiwan on the bioactive compounds obtained from Vietnamese plants and fungi. Among the tested compounds, hexatenuin A displayed the most significant inhibition of superoxide anion generation and elastase release. These triterpenoids may be used as potential anti-inflammatory agents.
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- 2017
- Full Text
- View/download PDF
47. Estrogenic and anti-neutrophilic inflammatory phenanthrenes from Juncus effusus L
- Author
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Yu Ting Kuo, Fang Rong Chang, Yi Hong Tsai, Szu Yin Yu, Tsong-Long Hwang, Chih Chan Lin, Yang Chang Wu, Ferenc Fülöp, Kuei Hung Lai, Yu Ming Hsu, Hao Chun Hu, and Yu Che Chuang
- Subjects
biology ,medicine.drug_class ,Chemistry ,Superoxide ,Organic Chemistry ,Elastase ,Estrogen receptor ,Plant Science ,Traditional Chinese medicine ,Pharmacology ,biology.organism_classification ,Biochemistry ,Anti-inflammatory ,Analytical Chemistry ,chemistry.chemical_compound ,Edema ,Juncus ,medicine ,medicine.symptom ,Phenanthrenes - Abstract
Juncus effusus L. (J. effusus) is a Traditional Chinese Medicine (TCM) that has long been used for dealing with gynaecological disorders, such as relieving insomnia, preventing tinnitus, reducing edema with diuretic effect. In our course of evidence-based medical research focused on this herb, one new phenanthrene, Junfusol B (2), together with seventeen known compounds were isolated and identified. All the structures of these compounds were elucidated by spectroscopic methods. The absolute stereochemistry of compounds 1 and 2 was further determined by comparing their calculated and experimental Electronic Circular Dichroism (ECD) spectra and optical rotation (OR) values. The isolates were evaluated for their estrogenic and anti-inflammatory activities which were considered as relevant etiological factors of insomnia, tinnitus and edema in the ancient TCM theory. The results revealed that most of the obtained phenanthrenes in this work were found exerting agonistic effects on estrogen receptor. This is the first report to declare the exact estrogen-regulating potential among this type of compounds from J. effusus. Moreover, phenanthrenes 3 - 7 exhibited significant inhibitions on superoxide anion generation and elastase release in fMLP/CB-induced human neutrophilic inflammation model. J. effusus may be developed as a complementary agent utilized in menopausal multiple syndromes.
- Published
- 2021
48. Targeting
- Author
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Wei, Wuli, Shinn-Zong, Lin, Shee-Ping, Chen, Bakhos A, Tannous, Wen-Sheng, Huang, Peng Yeong, Woon, Yang-Chang, Wu, Hsueh-Hui, Yang, Yi-Cheng, Chen, Renata Lopes, Fleming, Jack T, Rogers, Catherine M, Cahill, Tsung-Jung, Ho, Tzyy-Wen, Chiou, and Horng-Jyh, Harn
- Subjects
Mice ,MicroRNAs ,Amyloid beta-Peptides ,Cognition ,Alzheimer Disease ,Presenilin-1 ,Animals ,Biotin ,Plaque, Amyloid ,RNA, Long Noncoding ,Amyloid Precursor Protein Secretases - Published
- 2022
49. Hericium erinaceus Mycelium Ameliorates In Vivo Progression of Osteoarthritis
- Author
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Shang-Yu Yang, Chi-Jung Fang, Yu-Wen Chen, Wan-Ping Chen, Li-Ya Lee, Chin-Chu Chen, Yen-You Lin, Shan-Chi Liu, Chun-Hao Tsai, Wei-Chien Huang, Yang-Chang Wu, and Chih-Hsin Tang
- Subjects
osteoarthritis ,Hericium erinaceus ,mycelium ,anterior cruciate ligament transection ,interleukin 1 beta ,tumor necrosis factor-alpha ,Nutrition and Dietetics ,fungi ,Food Science - Abstract
Osteoarthritis (OA) is an age-related disorder that affects the joints and causes functional disability. Hericium erinaceus is a large edible mushroom with several known medicinal functions. However, the therapeutic effects of H. erinaceus in OA are unknown. In this study, data from Sprague-Dawley rats with knee OA induced by anterior cruciate ligament transection (ACLT) indicated that H. erinaceus mycelium improves ACLT-induced weight-bearing asymmetry and minimizes pain. ACLT-induced increases in articular cartilage degradation and bone erosion were significantly reduced by treatment with H. erinaceus mycelium. In addition, H. erinaceus mycelium reduced the synthesis of proinflammatory cytokines interleukin-1β and tumor necrosis factor-α in OA cartilage and synovium. H. erinaceus mycelium shows promise as a functional food in the treatment of OA.
- Published
- 2022
- Full Text
- View/download PDF
50. The Use of San-Huang-Xie-Xin-Tang Reduces the Mortality Rate among Breast Cancer Patients
- Author
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Daniel Winardi, Chieh-Hsin Wu, Jen-Huai Chiang, Yung-Hsiang Chen, Ching-Liang Hsieh, Juan-Cheng Yang, and Yang-Chang Wu
- Subjects
Cancer Research ,breast cancer ,Oncology ,population ,San-Huang-Xie-Xin-Tang (SHXXT) ,mortality ,risk - Abstract
Globally, breast cancer is the most common cause of cancer deaths. In Taiwan, it is the most prevalent cancer among females. Since San-Huang-Xie-Xin-Tang (SHXXT) exerts not only an anti-inflammatory but an immunomodulatory effect, it may act as a potent anti-tumor agent. Herein, the study aimed to explore the influence of SHXXT and its constituents on the mortality rate among breast cancer patients in Taiwan regarding the component effect and the dose–relationship effect. By using the Taiwan National Health Insurance (NHI) Research Database (NHIRD), the study analyzed 5387 breast cancer patients taking Chinese herbal medicine (CHM) and 5387 breast cancer patients not using CHM. CHM means SHXXT and its constituents in the study. The Kaplan–Meier method was utilized to determine the mortality probabilities among patients. Whether the CHM influences the mortality rate among patients was estimated by Cox proportional hazard regression analysis. The use of CHM could lower the cancer mortality rate by 59% in breast cancer patients. The protective effect was parallel to the cumulative days of CHM use and the annual average CHM dose. In addition, the mortality rate was lower in patients who used SHXXT compared to those who only used one of its constituents. SHXXT and its constituents were all promising therapeutic weapons against breast cancer.
- Published
- 2023
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