50 results on '"Yanan Wen"'
Search Results
2. A global record of annual terrestrial Human Footprint dataset from 2000 to 2018
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Haowei Mu, Xuecao Li, Yanan Wen, Jianxi Huang, Peijun Du, Wei Su, Shuangxi Miao, and Mengqing Geng
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Science - Abstract
Measurement(s) human footprint Technology Type(s) remote sensing Factor Type(s) Built Environment • Population Density • Nighttime lights • Croplands • Pastures • Roads • Railways • Navigable waterways
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- 2022
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3. Mapping crop type in Northeast China during 2013–2021 using automatic sampling and tile-based image classification
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Fu Xuan, Yi Dong, Jiayu Li, Xuecao Li, Wei Su, Xianda Huang, Jianxi Huang, Zixuan Xie, Ziqian Li, Hui Liu, Wancheng Tao, Yanan Wen, and Ying Zhang
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Crop mapping ,Google Earth Engine ,Time-series ,Tile-based classification ,Random forest ,Physical geography ,GB3-5030 ,Environmental sciences ,GE1-350 - Abstract
Northeast China is one of the most major grain banks in China and has an overwhelming influence on food security. To mitigate the challenges caused by increasing food demands and soil protection, crop rotation and fallowing policies have been introduced in Northeast China. These soil protection policies change annual crop planting area and crop distribution. To monitor crop type and its changes on a regional scale in time series, we explore the automatic sampling approach by hexagon strategy and tile-based classification by random forest (RF) algorithm using time-series Landsat-8 Operational Land Imager (OLI) images during 2013–2021. The crop maps have high credibility with the overall accuracies (OA) wall-to-wall ranging from 0.89 to 0.97, and also have close agreement with statistical data city by city. This study provides a highly reliable long-term crop maps dataset, which can be helpful for food security and regional agricultural production management.
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- 2023
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4. Transcriptome-Wide Analysis of RNA N6-Methyladenosine Modification in Adriamycin-Resistant Acute Myeloid Leukemia Cells
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Shu Fang, Bo Peng, Yanan Wen, Jingjing Yang, Hao Wang, Ziwei Wang, Kun Qian, Yan Wei, Yifan Jiao, Chunji Gao, and Liping Dou
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acute myeloid leukemia ,drug resistance ,N6-methyladenosine ,gene expression ,METTL3 ,Genetics ,QH426-470 - Abstract
Acute myeloid leukemia (AML) is one of the most aggressive hematopoietic malignancies. Patients still suffer from refractory/relapsed disease after anthracycline-based therapy, which leads to a poor prognosis. N6-Methyladenosine (m6A) is the most abundant post-transcriptional modification in eukaryotes, the imbalance of which is reported to be associated with various pathological processes, including drug resistance. However, the relationship between m6A modification and drug resistance has not been well defined in AML. In this study, we analyzed the sequencing data of HL60 and its Adriamycin-resistant cell line HL60/ADR. We found a total of 40,550 m6A-methylated peaks, representing 15,640 genes in HL60, and 38,834 m6A-methylated peaks, representing 15,285 genes in HL60/ADR. KEGG pathway analysis showed that pathways were enriched in the FoxO signaling pathway, p53 signaling pathway, and Notch signaling pathway. MeRIP-seq results showed that the fold enrichment of the global m6A level in HL60/ADR was higher than that in HL60, and dot blot assay results indicated that the global m6A level was elevated in HL60/ADR cells compared with that in HL60 cells. Further analysis revealed that the expression level of METTL3 was elevated in HL60/ADR cells compared with that in HL60 cells. After a combined treatment of STM2457 (an inhibitor of METTL3) and Adriamycin, the proliferation of HL60/ADR was inhibited. Thus, we hypothesized that the abnormality of m6A modification played an important role in Adriamycin-resistant AML.
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- 2022
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5. Study on the Influence of Low-Price Bid Winning and General Subcontracting Management on the Unsafe Behavior Intention of Construction Workers
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Jinbao Yao, Zhaozhi Wu, Yanan Wen, and Zixuan Peng
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low-price bid winning ,general subcontracting management ,unsafe behavior intention ,mediating effect analysis ,multiple regression model ,Psychology ,BF1-990 - Abstract
In recent years, there are many reasons for the frequent safety accidents in the construction field. The most controversial and typical one that firmly correlated with China’s national condition is the low-price bid winning and the general subcontracting management, which probably have a great impact on the unsafe behavior intention of workers on the construction site. In order to figure out their internal relation, a quantitative statistical analysis of the unsafe behavior intentions of construction workers in the Beijing area was conducted through the on-site questionnaire considering three main variables, namely, general subcontract management, reasonable low-cost bid winning, and construction experiences. Meanwhile, the correlation, regression, and mediating effects of different influencing factors were analyzed through a regressive model to quantify the impact of each variable on the unsafe behavior intention of construction workers. The results showed that the influence of low-price bid winning on the unsafe behavior intention of on-site workers is faint. This is mainly because, in the case of labor buyer’s market, the actual salary of workers is not relevant to whether the project is awarded at a low price. However, the general subcontracting management has a great impact on the unsafe behavior intention of on-site workers. At the same time, low-price bid winning also indirectly affects the strength of general subcontracting safety management, which has an indirect impact on the unsafe behavior intention of on-site workers. Generally, it is of greater significance to enhance the strength of the general subcontracting management and to formulate relevant regulations to guarantee the safety of construction workers.
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- 2022
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6. Monitoring Ground Surface Deformation of Ice-Wedge Polygon Areas in Saskylakh, NW Yakutia, Using Interferometric Synthetic Aperture Radar (InSAR) and Google Earth Engine (GEE)
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Wenhui Wang, Huijun Jin, Ze Zhang, Mikhail N. Zhelezniak, Valentin V. Spektor, Raul-David Șerban, Anyuan Li, Vladimir Tumskoy, Xiaoying Jin, Suiqiao Yang, Shengrong Zhang, Xiaoying Li, Mihaela Șerban, Qingbai Wu, and Yanan Wen
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climate change ,ice-wedge polygon (IWP) ,ground surface deformation (GSD) ,interferometric synthetic aperture radar (InSAR) ,Google Earth Engine (GEE) ,Russian Arctic ,Science - Abstract
As one of the best indicators of the periglacial environment, ice-wedge polygons (IWPs) are important for arctic landscapes, hydrology, engineering, and ecosystems. Thus, a better understanding of the spatiotemporal dynamics and evolution of IWPs is key to evaluating the hydrothermal state and carbon budgets of the arctic permafrost environment. In this paper, the dynamics of ground surface deformation (GSD) in IWP zones (2018–2019) and their influencing factors over the last 20 years in Saskylakh, northwestern Yakutia, Russia were investigated using the Interferometric Synthetic Aperture Radar (InSAR) and Google Earth Engine (GEE). The results show an annual ground surface deformation rate (AGSDR) in Saskylakh at −49.73 to 45.97 mm/a during the period from 1 June 2018 to 3 May 2019. All the selected GSD regions indicate that the relationship between GSD and land surface temperature (LST) is positive (upheaving) for regions with larger AGSDR, and negative (subsidence) for regions with lower AGSDR. The most drastic deformation was observed at the Aeroport regions with GSDs rates of −37.06 mm/a at tower and 35.45 mm/a at runway. The GSDs are negatively correlated with the LST of most low-centered polygons (LCPs) and high-centered polygons (HCPs). Specifically, the higher the vegetation cover, the higher the LST and the thicker the active layer. An evident permafrost degradation has been observed in Saskylakh as reflected in higher ground temperatures, lusher vegetation, greater active layer thickness, and fluctuant numbers and areal extents of thermokarst lakes and ponds.
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- 2023
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7. Seasonal Variations of Dissolved Organic Matter in Urban Rivers of Northern China
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Yanan Wen, Min Xiao, Zhaochuan Chen, Wenxi Zhang, and Fujun Yue
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dissolved organic matter ,absorption parameters ,land use ,urban rivers ,human activity ,Agriculture - Abstract
Dissolved organic matter (DOM) is ubiquitously present in aquatic environments, playing an important role in the global carbon cycle and water quality. It is necessary to reveal the potential sources and explore spatiotemporal variation of DOM in rivers, especially in urban zones impacted by human activities. It was designed to aim to explore spatiotemporal variations of DOM in urban rivers and ascertain the influencing factors. In this study, dissolved organic carbon (DOC) concentrations, UV-Vis absorption spectroscopy, and 3D fluorescence spectroscopy combined with parallel factor analysis were utilized to characterize DOM composition in urban rivers (the Jiyun, Chaobai, and Yongding rivers) in Tianjin city, northern China. The results showed that DOC (1.28 to 25.85 mg·L−1), generally, was at its highest level in spring, followed by summer, and lowest in autumn and winter, and that the absorption parameters E250:365 (condensation degree/molecular weight, 7.88), SUVA254 (aromaticity, 3.88 L mg C−1 m−1), a355 (content of chromophores, 4.34 m−1), a260 (hydrophobicity, 22.02 m−1), and SR (molecular weight, 1.08) of CDOM (chromophoric DOM) suggested that DOM is mainly composed of low-molecular-weight fulvic acid and protein-like moieties, and had the capability of participating in pollutant migrations and transformations. The results demonstrated significant seasonal differences. Generally, high DOC content was detected in rivers in urban suburbs, due to anthropogenic inputs. Three fluorescence components were identified, and the fluorescence intensity of the protein class reached the highest value, 294.47 QSU, in summer. Different types of land use have different effects on the compositions of riverine DOM; more protein-like DOM was found in sections of urban rivers. The correlation between DOC concentration and the CDOM absorption coefficient was found to be unstable due to deleterious input from industrial and agricultural wastewater and from domestic sewage from human activities. HIX and BIX elucidated that the source of CDOM in three river watersheds was influenced by both terrestrial and autochthonous sources, and the latter prevailed over the former. Geospatial data analysis indicated that CDOM in autumn was sourced from plant detritus degradation from forest land or from the urban green belt; construction land had a great influence on DOC and CDOM in riparian buffer areas. It was revealed that DOM in the watershed is highly impacted by nature and human activities through land use, soil erosion, and surface runoff/underground percolation transport; domestic sewage discharge constituted the primary source and was the greatest determiner among the impacts.
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- 2023
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8. The invasive MED/Q Bemisia tabaci genome: a tale of gene loss and gene gain
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Wen Xie, Xin Yang, Chunhai Chen, Zezhong Yang, Litao Guo, Dan Wang, Jinqun Huang, Hailin Zhang, Yanan Wen, Jinyang Zhao, Qingjun Wu, Shaoli Wang, Brad S. Coates, Xuguo Zhou, and Youjun Zhang
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Bemisia tabaci ,MED/Q ,Invasive species ,Genome ,Gene gain and loss ,Detoxification enzymes ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Sweetpotato whitefly, Bemisia tabaci MED/Q and MEAM1/B, are two economically important invasive species that cause considerable damages to agriculture crops through direct feeding and indirect vectoring of plant pathogens. Recently, a draft genome of B. tabaci MED/Q has been assembled. In this study, we focus on the genomic comparison between MED/Q and MEAM1/B, with a special interest in MED/Q’s genomic signatures that may contribute to the highly invasive nature of this emerging insect pest. Results The genomes of both species share similarity in syntenic blocks, but have significant divergence in the gene coding sequence. Expansion of cytochrome P450 monooxygenases and UDP glycosyltransferases in MED/Q and MEAM1/B genome is functionally validated for mediating insecticide resistance in MED/Q using in vivo RNAi. The amino acid biosynthesis pathways in MED/Q genome are partitioned among the host and endosymbiont genomes in a manner distinct from other hemipterans. Evidence of horizontal gene transfer to the host genome may explain their obligate relationship. Putative loss-of-function in the immune deficiency-signaling pathway due to the gene loss is a shared ancestral trait among hemipteran insects. Conclusions The expansion of detoxification genes families, such as P450s, may contribute to the development of insecticide resistance traits and a broad host range in MED/Q and MEAM1/B, and facilitate species’ invasions into intensively managed cropping systems. Numerical and compositional changes in multiple gene families (gene loss and gene gain) in the MED/Q genome sets a foundation for future hypothesis testing that will advance our understanding of adaptation, viral transmission, symbiosis, and plant-insect-pathogen tritrophic interactions.
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- 2018
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9. Evaluation of Light Pollution in Global Protected Areas from 1992 to 2018
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Haowei Mu, Xuecao Li, Xiaoping Du, Jianxi Huang, Wei Su, Tengyun Hu, Yanan Wen, Peiyi Yin, Yuan Han, and Fei Xue
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nighttime light ,urban sprawl ,nighttime light intensity ,biodiversity conservation ,Science - Abstract
Light pollution, a phenomenon in which artificial nighttime light (NTL) changes the form of brightness and darkness in natural areas such as protected areas (PAs), has become a global concern due to its threat to global biodiversity. With ongoing global urbanization and climate change, the light pollution status in global PAs deserves attention for mitigation and adaptation. In this study, we developed a framework to evaluate the light pollution status in global PAs, using the global NTL time series data. First, we classified global PAs (30,624) into three pollution categories: non-polluted (5974), continuously polluted (8141), and discontinuously polluted (16,509), according to the time of occurrence of lit pixels in/around PAs from 1992 to 2018. Then, we explored the NTL intensity (e.g., digital numbers) and its trend in those polluted PAs and identified those hotspots of PAs at the global scale with consideration of global urbanization. Our study shows that global light pollution is mainly distributed within the range of 30°N and 60°N, including Europe, north America, and East Asia. Although the temporal trend of NTL intensity in global PAs is increasing, Japan and the United States of America (USA) have opposite trends due to the implementation of well-planned ecological conservation policies and declining population growth. For most polluted PAs, the lit pixels are close to their boundaries (i.e., less than 10 km), and the NTL in/around these lit areas has become stronger over the past decades. The identified hotspots of PAs (e.g., Europe, the USA, and East Asia) help support decisions on global biodiversity conservation, particularly with global urbanization and climate change.
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- 2021
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10. Hourly PM2.5 Estimation over Central and Eastern China Based on Himawari-8 Data
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Yong Xue, Ying Li, Jie Guang, Alexandru Tugui, Lu She, Kai Qin, Cheng Fan, Yahui Che, Yanqing Xie, Yanan Wen, and Zixiang Wang
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himawari-8 ,aod ,pm2.5 ,igtwr ,china ,hourly ,Science - Abstract
In this study, an improved geographically and temporally weighted regression (IGTWR) model for the estimation of hourly PM2.5 concentration data was applied over central and eastern China in 2017, based on Himawari-8 Advanced Himawari Imager (AHI) data. A generalized distance based on the longitude, latitude, day, hour, and land use type was constructed. AHI aerosol optical depth, surface relative humidity, and boundary layer height (BLH) data were used as independent variables to retrieve the hourly PM2.5 concentrations at 1:00, 2:00, 3:00, 4:00, 5:00, 6:00, 7:00, and 8:00 UTC (Coordinated Universal Time). The model fitting and cross-validation performance were satisfactory. For the model fitting set, the correlation coefficient of determination (R2) between the measured and predicted PM2.5 concentrations was 0.886, and the root-mean-square error (RMSE) of 437,642 samples was only 12.18 µg/m3. The tenfold cross-validation results of the regression model were also acceptable; the correlation coefficient R2 of the measured and predicted results was 0.784, and the RMSE was 20.104 µg/m3, which is only 8 µg/m3 higher than that of the model fitting set. The spatial and temporal characteristics of the hourly PM2.5 concentration in 2017 were revealed. The model also achieved stable performance under haze and dust conditions.
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- 2020
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11. Empirical Study on Job Satisfaction in the Career Development of Civil Servants at the Grass-roots Level—Based on Questionnaires in Six Counties in Anhui Province
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Yanan Wen, Feng Zheng, and Yanhan Chen
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- 2023
12. Structure and function of a bacterial type III-E CRISPR–Cas7-11 complex
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Guimei Yu, Xiaoshen Wang, Yi Zhang, Qiyin An, Yanan Wen, Xuzichao Li, Hang Yin, Zengqin Deng, and Heng Zhang
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Microbiology (medical) ,Immunology ,Genetics ,Cell Biology ,Applied Microbiology and Biotechnology ,Microbiology - Published
- 2022
13. Mapping corn dynamics using limited but representative samples with adaptive strategies
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Yanan Wen, Xuecao Li, Haowei Mu, Liheng Zhong, Han Chen, Yelu Zeng, Shuangxi Miao, Wei Su, Peng Gong, Baoguo Li, and Jianxi Huang
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Computers in Earth Sciences ,Engineering (miscellaneous) ,Atomic and Molecular Physics, and Optics ,Computer Science Applications - Published
- 2022
14. Characteristics of Dissolved Organic Matter Impacted by Different Land Use in Haihe River Watershed, China
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Zhaochuan Chen, Yanan Wen, Min Xiao, Fujun Yue, and Wenxi Zhang
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Health, Toxicology and Mutagenesis ,source distribution ,seasonal variations ,Public Health, Environmental and Occupational Health ,EEM-PARAFAC analysis model ,land use ,dissolved organic matter ,Haihe River - Abstract
It is important to explore characteristics of dissolved organic matter (DOM) in the riverine system due to its critical role in the carbon cycle. This study investigated the distribution characteristics and sources of DOM based on excitation emission matrix three-dimensional fluorescence technology and parallel factor (EEM-PARAFAC) analysis at two rivers in northern China strongly impacted by human activities. The results show that the fluorescence intensity of terrestrial humic-like substances increased during summer in Haihe River. The intensity was significantly higher than in spring due to terrestrial detritus from runoff conveyance. The fluorescence intensity of protein-like substances in spring was the highest and decreased in summer. This feature of DOM in the Duliujian River was related to the increase in precipitation and surface runoff in the wet season and the rapid degradation of mixed DOM in the dry season. An analysis of HIX, BIX and FI showed a low degree of DOM humification and more endogenous contributions from microbial and phytoplankton degradation. Seasonal variations of dissolved organic carbon (DOC) and chromophoric DOM (CDOM, a335, thereinto C1) suggest that chromophores, particularly terrestrial substances, regulate the temporal patterns of DOM in the two rivers. Combined with the analysis of the proportion of land use types in riparian buffers, tillage had a great impact on DOM content and hydrophobicity in Haihe River watershed. Domestic wastewater and industrial sewage discharge contribute more DOM to Duliujian River watershed, which was indicated by more abundant protein-like components (212.17 ± 94.63 QSU in Duliujian River;186.59 ± 238.72 QSU in Haihe River). This study highlights that different land use types resulted in distinctive sources and seasonal dynamics of DOM in rivers. Meanwhile, it should be considered that the estimation of carbon cycling should involve monitoring and evaluating anthropogenic inputs into rivers.
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- 2023
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15. Target RNA-guided protease activity in type III-E CRISPR-Cas system
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Xiaoshen Wang, Guimei Yu, Yanan Wen, Qiyin An, Xuzichao Li, Fumeng Liao, Chengwei Lian, Kai Zhang, Hang Yin, Yong Wei, Zengqin Deng, and Heng Zhang
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Genetics - Abstract
The type III-E CRISPR–Cas systems are newly identified adaptive immune systems in prokaryotes that use a single Cas7–11 protein to specifically cleave target RNA. Cas7–11 could associate with Csx29, a putative caspase-like protein encoded by the gene frequently found in the type III-E loci, suggesting a functional linkage between the RNase and protease activities in type III-E systems. Here, we demonstrated that target RNA recognition would stimulate the proteolytic activity of Csx29, and protein Csx30 is the endogenous substrate. More interestingly, while the cognate target RNA recognition would activate Csx29, non-cognate target RNA with the complementary 3′ anti-tag sequence inhibits the enzymatic activity. Csx30 could bind to the sigma factor RpoE, which may initiate the stress response after proteolytic cleavage. Combined with biochemical and structural studies, we have elucidated the mechanisms underlying the target RNA-guided proteolytic activity of Csx29. Our work will guide further developments leveraging this simple RNA targeting system for RNA and protein-related applications.
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- 2022
16. Reduced Risk of Chronic Graft-Versus-Host Disease (cGVHD) by Rabbit Anti-Thymocyte Globulin (ATG) in Patients Undergoing Matched Sibling Donor Transplantation in Hematological Malignancies
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Shu Fang, Nan Wang, Lili Wang, Jishan Du, Jingjing Yang, Yanan Wen, Yan Wei, Kun Qian, Hao Wang, Yifan Jiao, Chunji Gao, and Liping Dou
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Transplantation ,Transplantation Conditioning ,Hematologic Neoplasms ,Siblings ,Hematopoietic Stem Cell Transplantation ,Graft vs Host Disease ,Humans ,General Medicine ,Neoplasm Recurrence, Local ,Antilymphocyte Serum ,Retrospective Studies - Abstract
BACKGROUND With the addition of anti-thymocyte globulin (ATG) to GVHD prophylaxis in patients undergoing transplantation of peripheral blood stem cells (PBSCT), the incidence of cGVHD decreases. However, the optimal dose and timing of ATG remain undetermined. MATERIAL AND METHODS In this historical controlled trial, data from 85 patients who had hematological malignancies and underwent matched sibling donor (MSD)-PBSCT were used to analyze the effectiveness of rabbit ATG (rATG) for prophylaxis of GVHD. Forty patients received 5 mg/kg rATG used for days -5 to -2, and 45 patients did not receive ATG. RESULTS All patients had successful engraftment except for 2 in the non-ATG group, who had platelet engraftment failure. The 2-year cumulative incidence of chronic GVHD (cGVHD) in the ATG group versus non-ATG group was 19.3% (95% CI, 8.4-33.6%) versus 61.4% (95% CI, 45.4-73.9%) (P0.001), and in those with moderate to severe cGVHD it was 11.0% (95% CI, 3.4-23.6%) versus 31.8% (95% CI, 18.8-45.6%) (P=0.029), respectively. The 2-year cumulative incidence of non-relapse mortality and relapse (CIR) were 0% versus 15.5% (95% CI, 6.8-27.5%) (P=0.018), and 53.3% (95% CI, 35.6-68.1%) versus 26.7% (95% CI, 14.9-40.0%) (P=0.019), respectively. No differences were found in other survival outcomes. In the multivariate analysis, ATG was an independent protective factor for moderate to severe cGVHD (HR=0.314, 95% CI, 0.103-0.958, P=0.042), and was an independent poor risk factor for CIR (HR=2.337, 95% CI, 1.133-4.822, P=0.022). CONCLUSIONS ATG in our strategy was effective for prophylaxis of cGVHD, whereas the relapse rate was increased in patients with rATG.
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- 2022
17. A C-terminal glutamine recognition mechanism revealed by E3 ligase TRIM7 structures
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Xiao Liang, Jun Xiao, Xuzichao Li, Yujie Liu, Yao Lu, Yanan Wen, Zexing Li, Xing Che, Yongjian Ma, Xingyan Zhang, Yi Zhang, Deng Jian, Peihui Wang, Chenghao Xuan, Guimei Yu, Long Li, and Heng Zhang
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Tripartite Motif Proteins ,SARS-CoV-2 ,Ubiquitin-Protein Ligases ,Glutamine ,Ubiquitination ,Humans ,COVID-19 ,Cell Biology ,Molecular Biology ,Antiviral Agents - Abstract
The E3 ligase TRIM7 has emerged as a critical player in viral infection and pathogenesis. However, the mechanism governing the TRIM7-substrate association remains to be defined. Here we report the crystal structures of TRIM7 in complex with 2C peptides of human enterovirus. Structure-guided studies reveal the C-terminal glutamine residue of 2C as the primary determinant for TRIM7 binding. Leveraged by this finding, we identify norovirus and SARS-CoV-2 proteins, and physiological proteins, as new TRIM7 substrates. Crystal structures of TRIM7 in complex with multiple peptides derived from SARS-CoV-2 proteins display the same glutamine-end recognition mode. Furthermore, TRIM7 could trigger the ubiquitination and degradation of these substrates, possibly representing a new Gln/C-degron pathway. Together, these findings unveil a common recognition mode by TRIM7, providing the foundation for further mechanistic characterization of antiviral and cellular functions of TRIM7.
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- 2022
18. Optimization of cationic polymer-mediated transfection for RNA interference
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Xiaojie Fan, Jingnan Yang, Guangyao Wu, Meiyi Wang, Xiaoxia Cheng, Chang Liu, Qian Liu, Yanan Wen, Shuangshuang Meng, Zhenxing Wang, Xuhong Lin, and Lei An
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animal structures ,shRNA ,viruses ,embryonic structures ,fungi ,cationic polymer ,Genetics ,transfection reagent ,RNAi ,Molecular Biology - Abstract
Transfection efficiency was estimated to optimize the conditions for RNA interference (RNAi), including transfection time, validity, and nucleic acid concentration and type, using the EZ Trans Cell Reagent, a cationic polymer. An shRNA against GFP was designed and transfected into cells using the EZ transfection reagent. The shRNA significantly decreased the expression of GFP. In addition, pre-diluted transfection reagent at room temperature and small nucleic acids increased the transfection efficiency, which peaked at 24 h. Compared with circular nucleic acids, linear nucleic acids showed higher transfection efficiency and a higher genome integration rate. We optimized cationic polymer-mediated RNAi conditions, and our data will be useful for future RNAi studies.
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- 2022
19. Investigation of imidazole derivatives as corrosion inhibitors of copper in sulfuric acid: Combination of experimental and theoretical researches
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Yanan Wen, Li Feng, Bochuan Tan, Jida Chen, Wenpo Li, Denglin Fu, Yujie Qiang, Lu Lansi, Shengtao Zhang, and Yan Ting
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Materials science ,General Chemical Engineering ,Inorganic chemistry ,chemistry.chemical_element ,Langmuir adsorption model ,Sulfuric acid ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Copper ,0104 chemical sciences ,Corrosion ,Corrosion inhibitor ,chemistry.chemical_compound ,symbols.namesake ,Adsorption ,chemistry ,Physisorption ,Chemisorption ,symbols ,0210 nano-technology - Abstract
Levamisole (LMS) and 4-phenylimidazole (PIZ), used as corrosion inhibitors of copper in sulfuric acid solution were explored by electrochemical tests, morphology analysis and theoretical calculation. At the concentration of 8 mM, the maximum corrosion inhibition efficiencies of LMS and PIZ are 99.03% and 95.84%, respectively. All test data found that LMS had better corrosion inhibition performance than PIZ. LMS is a cathodic corrosion inhibitor, while PIZ belongs to a mixed-type corrosion inhibitor. Electrochemical results indicate that the corrosion inhibition efficiency has the same trend when the concentration of corrosion inhibitor increases. Scanning electron microscope and atomic force microscope were applied to research the surface morphology of copper samples under different conditions. X-ray photoelectron spectroscopy and Langmuir adsorption isotherm model were utilized to explain adsorption means. What is more, Langmuir adsorption isotherm model indicates the coexistence of physisorption and chemisorption for the two inhibitors. Besides, the adsorption between LMS and copper is more prone to chemical adsorption. The mechanism of metallic copper and corrosion inhibitors was explored through quantum chemistry studies and molecular dynamics simulation.
- Published
- 2020
20. Transcriptome-Wide Analysis of RNA N
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Shu, Fang, Bo, Peng, Yanan, Wen, Jingjing, Yang, Hao, Wang, Ziwei, Wang, Kun, Qian, Yan, Wei, Yifan, Jiao, Chunji, Gao, and Liping, Dou
- Abstract
Acute myeloid leukemia (AML) is one of the most aggressive hematopoietic malignancies. Patients still suffer from refractory/relapsed disease after anthracycline-based therapy, which leads to a poor prognosis. N
- Published
- 2021
21. Can Financial Shared Services Improve Business Performance? Evidence from Chinese A-share Listed Corporations
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Yihan Wang and Yanan Wen
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Finance ,business.industry ,Business ,A share - Published
- 2021
22. Effects of 2,2-Dithiodipyridine as a Leveler for Through-Holes Filling by Copper Electroplating
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Yujie Qiang, Yanan Wen, Shijin Chen, Guo Hailiang, Shulei Fu, Shengtao Zhang, Bochuan Tan, Y.Q. Wang, and Wang Xu
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Materials science ,Leveler ,Renewable Energy, Sustainability and the Environment ,Metallurgy ,Materials Chemistry ,Electrochemistry ,Copper plating ,Condensed Matter Physics ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2019
23. Hourly PM2.5 Estimation over Central and Eastern China Based on Himawari-8 Data
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Kai Qin, Zixiang Wang, Cheng Fan, Yanqing Xie, Alexandru Tugui, Yong Xue, Ying Li, Lu She, Yahui Che, Yanan Wen, and Jie Guang
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Haze ,010504 meteorology & atmospheric sciences ,Mean squared error ,Correlation coefficient ,Science ,aod ,Regression analysis ,010501 environmental sciences ,Atmospheric sciences ,01 natural sciences ,igtwr ,Latitude ,Himawari-8 ,AOD ,PM2.5 ,IGTWR ,China ,hourly ,himawari-8 ,General Earth and Planetary Sciences ,Environmental science ,pm2.5 ,Relative humidity ,china ,Longitude ,Unit-weighted regression ,0105 earth and related environmental sciences - Abstract
In this study, an improved geographically and temporally weighted regression (IGTWR) model for the estimation of hourly PM2.5 concentration data was applied over central and eastern China in 2017, based on Himawari-8 Advanced Himawari Imager (AHI) data. A generalized distance based on the longitude, latitude, day, hour, and land use type was constructed. AHI aerosol optical depth, surface relative humidity, and boundary layer height (BLH) data were used as independent variables to retrieve the hourly PM2.5 concentrations at 1:00, 2:00, 3:00, 4:00, 5:00, 6:00, 7:00, and 8:00 UTC (Coordinated Universal Time). The model fitting and cross-validation performance were satisfactory. For the model fitting set, the correlation coefficient of determination (R2) between the measured and predicted PM2.5 concentrations was 0.886, and the root-mean-square error (RMSE) of 437,642 samples was only 12.18 µg/m3. The tenfold cross-validation results of the regression model were also acceptable; the correlation coefficient R2 of the measured and predicted results was 0.784, and the RMSE was 20.104 µg/m3, which is only 8 µg/m3 higher than that of the model fitting set. The spatial and temporal characteristics of the hourly PM2.5 concentration in 2017 were revealed. The model also achieved stable performance under haze and dust conditions.
- Published
- 2020
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24. 3-Amino-1-phenyl-2-pyrazoline-5-ketone as a heterobifunctional chromogenic reagent to derivatize reducing glycans for subsequent online LC/MS analysis
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Chengjian Wang, Linjuan Huang, Zhongfu Wang, Rendan Liu, Wanjun Jin, Yu Lu, and Yanan Wen
- Subjects
0301 basic medicine ,Spectrometry, Mass, Electrospray Ionization ,Glycan ,Electrospray ionization ,Biophysics ,01 natural sciences ,Biochemistry ,Reductive amination ,High-performance liquid chromatography ,Glycomics ,03 medical and health sciences ,Polysaccharides ,Liquid chromatography–mass spectrometry ,Humans ,Pyrazolones ,Molecular Biology ,Chromatography ,biology ,Chemistry ,Chromogenic ,010401 analytical chemistry ,Cell Biology ,0104 chemical sciences ,030104 developmental biology ,Reagent ,biology.protein ,Chromatography, Liquid - Abstract
Sensitive analysis of glycans by liquid chromatography/mass spectrometry is significantly hampered by the lack of chromogenic or fluorescent groups on the glycan structures, as well as, their poor ionization properties. In the present, a heterobifunctional chromogenic reagent 3-amino-1-phenyl-2-pyrazoline-5-ketone (PAP) bearing amino and active methylene groups, which readily reacts with reducing glycans, was used for detection of the pre-column-labeled glycans via high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS). The PAP derivatives with active methylene and amino groups were obtained via reductive amination in acidic medium and condensation of an active PAP methylene group with the reducing end of glycans in alkaline medium, respectively, and the PAP derivatives could be further functionalized, e.g., via glycan microarray preparation. The conditions for the two reaction modes were optimized, the HPLC separation method of PAP derivatives was investigated, and the PAP derivatives of some glycans derived from biological samples were obtained and analyzed by ESI-MS and LC-MS. Using this new reagent, reducing glycans can be selectively derivatized by different reaction mechanisms, having great importance for functional glycomics studies.
- Published
- 2018
25. The Optical Characterization and Distribution of Dissolved Organic Matter in Water Regimes of Qilian Mountains Watershed
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Min Xiao, Zhaochuan Chen, Yuan Zhang, Yanan Wen, Lihai Shang, and Jun Zhong
- Subjects
Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Water ,dissolved organic matter ,spectroscopic technique ,Article ,mineral disintegration ,autochthonous generation ,Lakes ,Spectrometry, Fluorescence ,Water Quality ,Medicine ,Qilian Mountains watershed - Abstract
The constituents and content of dissolved organic matter (DOM) in the Qilian Mountain watershed were characterized with a spectroscopic technique, especially 3-DEEM fluorescence assisted by parallel factor (PARAFAC) analysis. The level of DOM in the surrounding area of Qinghai lake (thereafter the lake in this article specifically refers to Qinghai Lake)was highest at 9.45 mg C·L−1 and about 3 times less (3.09 mg C·L−1) in a cropland aquatic regime (the lowest value). In general, DOM was freshly autochthonously generated by plankton and plant debris, microorganisms and diagenetic effects in the aquatic environment (FI > 1.8). Component 1 (humic acid-like) and 3 (fulvic acid-like) determined the humification degree of chromophoric dissolved organic matter (CDOM). The spatial variation of sulfate and nitrate in the surrounding water regime of the lake revealed that organic molecules were mainly influenced by bacterial mediation. Mineral disintegration was an important and necessary process for fluorescent fraction formation in the cropland water regime. Exceptionally, organic moiety in the unused land area was affected by anespecially aridclimate in addition to microbial metabolic experience. Salinity became the critical factor determining the distribution of DOM, and the total normalized fluorescent intensity and CDOM level were lower in low-salinity circumstances (0.2–0.5 g·L−1) with 32.06 QSU and 1.38 m−1 in the grassland area, and higher salinity (0.6~0.8 g·L−1) resulted in abnormally high fluorescence of 150.62 QSU and absorption of 7.83 m−1 in the cropland water regime. Climatic conditions and microbial reactivity controlled by salinity were found to induce the above results. Our findings demonstrated that autochthonous inputs regulated DOM dynamics in the Qilian Mountains watershed of high altitude.
- Published
- 2021
26. Evaluation of Light Pollution in Global Protected Areas from 1992 to 2018
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Wei Su, Tengyun Hu, Fei Xue, Xiaoping Du, Xuecao Li, Yuan Han, Peiyi Yin, Jianxi Huang, Haowei Mu, and Yanan Wen
- Subjects
0106 biological sciences ,Pollution ,010504 meteorology & atmospheric sciences ,Science ,media_common.quotation_subject ,Light pollution ,Urban sprawl ,Climate change ,nighttime light ,010603 evolutionary biology ,01 natural sciences ,Geography ,urban sprawl ,Environmental protection ,nighttime light intensity ,Urbanization ,General Earth and Planetary Sciences ,Population growth ,East Asia ,biodiversity conservation ,0105 earth and related environmental sciences ,media_common ,Global biodiversity - Abstract
Light pollution, a phenomenon in which artificial nighttime light (NTL) changes the form of brightness and darkness in natural areas such as protected areas (PAs), has become a global concern due to its threat to global biodiversity. With ongoing global urbanization and climate change, the light pollution status in global PAs deserves attention for mitigation and adaptation. In this study, we developed a framework to evaluate the light pollution status in global PAs, using the global NTL time series data. First, we classified global PAs (30,624) into three pollution categories: non-polluted (5974), continuously polluted (8141), and discontinuously polluted (16,509), according to the time of occurrence of lit pixels in/around PAs from 1992 to 2018. Then, we explored the NTL intensity (e.g., digital numbers) and its trend in those polluted PAs and identified those hotspots of PAs at the global scale with consideration of global urbanization. Our study shows that global light pollution is mainly distributed within the range of 30°N and 60°N, including Europe, north America, and East Asia. Although the temporal trend of NTL intensity in global PAs is increasing, Japan and the United States of America (USA) have opposite trends due to the implementation of well-planned ecological conservation policies and declining population growth. For most polluted PAs, the lit pixels are close to their boundaries (i.e., less than 10 km), and the NTL in/around these lit areas has become stronger over the past decades. The identified hotspots of PAs (e.g., Europe, the USA, and East Asia) help support decisions on global biodiversity conservation, particularly with global urbanization and climate change.
- Published
- 2021
27. Glutathione S-transferases are involved in thiamethoxam resistance in the field whitefly Bemisia tabaci Q (Hemiptera: Aleyrodidae)
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Litao Guo, Youjun Zhang, Yanan Wen, Jixing Xia, Xiaomao Zhou, Fengshan Yang, Yating Liu, Chao He, Xin Yang, Qingjun Wu, Ze-Zong Yang, Shaoli Wang, and Wen Xie
- Subjects
Male ,0106 biological sciences ,0301 basic medicine ,Insecticides ,Pesticide resistance ,Health, Toxicology and Mutagenesis ,Bifenthrin ,Gene Expression ,Whitefly ,01 natural sciences ,Acetamiprid ,Carboxylesterase ,Microbiology ,Hemiptera ,Insecticide Resistance ,Neonicotinoids ,03 medical and health sciences ,chemistry.chemical_compound ,Cytochrome P-450 Enzyme System ,Imidacloprid ,Oxazines ,Botany ,Animals ,Glutathione Transferase ,Nitenpyram ,biology ,General Medicine ,Nitro Compounds ,biology.organism_classification ,Thiazoles ,010602 entomology ,030104 developmental biology ,chemistry ,Abamectin ,Insect Proteins ,Female ,RNA Interference ,Thiamethoxam ,Agronomy and Crop Science - Abstract
The whitefly, Bemisia tabaci, has developed a high level of resistance to thiamethoxam, a second generation neonicotinoid insecticide that has been widely used to control this pest. In this study, we assessed the level of cross-resistance, the activities of detoxifying enzymes, and the expression profiles of 23 glutathione S-transferase (GST) genes in a thiamethoxam-resistant ant and -susceptible strain of Bemisia tabaci Q. The thiamethoxam-resistant strain showed a moderate level of cross-resistance to another nicotinoid insecticide imidacloprid, a low level of cross-resistance to acetamiprid and nitenpyram, and no significant cross-resistance to abamectin and bifenthrin. Among detoxifying enzymes, only GSTs had significantly higher activity in the resistant strain than in the susceptible strain. Seven of 23 GST genes were over-expressed in the resistant strain relative to the susceptible strain. Using the technology of RNA interference to knockdown a GST gene (GST14), the results showed that silencing GST14 increased the mortality of whiteflies to thiamethoxam in Bemisia tabaci.
- Published
- 2016
28. Toxic effects of the Emamectin Benzoate exposure on cultured human bronchial epithelial (16HBE) cells
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Xiaobin Pang, Yanan Wen, Jing-Nan Yang, Lei An, Guangyao Wu, Xu-Hong Lin, Chunli Wang, Chenguang Niu, and Shuangshuang Meng
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Insecticides ,010504 meteorology & atmospheric sciences ,DNA damage ,Health, Toxicology and Mutagenesis ,Bronchi ,010501 environmental sciences ,Pharmacology ,Toxicology ,01 natural sciences ,chemistry.chemical_compound ,Toxicity Tests ,Bioassay ,Humans ,Viability assay ,Cytotoxicity ,0105 earth and related environmental sciences ,Emamectin ,Ivermectin ,biology ,Chemistry ,Cytochrome c ,General Medicine ,Pollution ,Apoptosis ,Toxicity ,biology.protein - Abstract
Pesticides pollution has caused serious environmental problems in recent years, and mounting evidence has shown that more and more insecticides have serious risk in human health. Emamectin Benzoate formally regarded as a highly safety insecticide based on its exclusive targets, but the cytotoxicity to human lung was ignored for a long time. In the present study, bioassay experiments were used to assess the toxicity of the Emamectin Benzoatein on human non-target cells including cell viability assay, DNA damage assay, flow cytometer assay and western blotting assay. The results indicated that Emamectin Benzoatecan cause the inhibition of the proliferation, cytochrome c release, activation of caspase-3/9 and increase Bax/Bcl-2 ratio, which means it induced the cytotoxicity on 16HBE cells associated with the mitochondrial apoptosis. Besides, the DNA damge caused by the Emamectin Benzoate suggest it has a potential genotoxic effect on human lung cells.
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- 2019
29. Ready-to-use strip for l-ascorbic acid visual detection based on polyaniline/polyamide 66 nano-fibers/nets membranes
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Li Faxue, Yang Si, Jianyong Yu, Yanan Wen, Bin Ding, Yan Li, and Xueqin Wang
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Aniline Compounds ,Chemistry ,Nanofibers ,Membranes, Artificial ,Ascorbic Acid ,Ascorbic acid ,Amides ,Electrospinning ,Analytical Chemistry ,Fruit and Vegetable Juices ,Standard curve ,chemistry.chemical_compound ,Membrane ,Chemical engineering ,Nanofiber ,Polyaniline ,Polymer chemistry ,Polyamide ,Colorimetry ,Food Analysis ,Reagent Strips - Abstract
A ready-to-use L-ascorbic acid (L-AA) colorimetric strip based on polyaniline (PANI)/polyamide 66 (PA 66) nanofiber/net (NFN) membrane with spider-web-like structure was fabricated through a facile bend electrospinning/netting process. Introduction of PANI component into the membrane turns the strip undergoes a brilliant brown-to-green color transition within 30 min upon incubation with L-AA. Moreover, ascribing to the three-dimensional microscopic structural, continuous pore channels, and distinctive nano-nets structure, the color change that is caused by 50 ppb L-AA can be easily perceived by naked eyes. Furthermore, RGB (red-green-blue) digital parameters that obtained from photographs of the strips were automatically read out via an iPhone. Subsequently, the parameters were converted into a color-difference map and processed statistically through principal component analysis, then used to elaborate standard curve. Combining the map and curve, we realized a method for assaying and quantifying L-AA concentration in real food samples, which avoids the time-consuming sample preparation, expensive laboratory techniques, and specialized personnel needed to carry out conventional analytical methods.
- Published
- 2015
30. Sensitive and robust MALDI-TOF-MS glycomics analysis enabled by Girard's reagent T on-target derivatization (GTOD) of reducing glycans
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Yuyang Zhu, Yanan Wen, Chengjian Wang, Zhongfu Wang, Mingming Yang, Linjuan Huang, Wanjun Jin, Ying Zhang, Lijing Nan, Bo Wang, Rendan Liu, and Xuezheng Song
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Glycan ,Oligosaccharides ,02 engineering and technology ,Mass spectrometry ,01 natural sciences ,Biochemistry ,Fucose ,Glycosphingolipids ,Article ,Analytical Chemistry ,Glycomics ,chemistry.chemical_compound ,Polysaccharides ,Environmental Chemistry ,Humans ,Derivatization ,Spectroscopy ,Glycoproteins ,Chromatography ,biology ,Milk, Human ,010401 analytical chemistry ,Reproducibility of Results ,Blood Proteins ,021001 nanoscience & nanotechnology ,Milk Proteins ,Fetuin ,0104 chemical sciences ,Sialic acid ,carbohydrates (lipids) ,Betaine ,Matrix-assisted laser desorption/ionization ,chemistry ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,biology.protein ,Sialic Acids ,Female ,0210 nano-technology - Abstract
Sensitive glycomics analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is of great importance but significantly hampered by their low ionization efficiency and labile sialic acid moieties. Chemical derivatization offers a viable way to improve both the ionization efficiency and analytical sensitivity of the glycans in MS analysis by altering their hydrophobicity or charge property. Here we employed Girard's reagent T (GT) for on-target derivatization (GTOD) of reducing glycan under mild acid condition to form stable hydrazones at room temperature, allowing rapid and sensitive identification of neutral and sialylated glycans in positive-ion mode as only permanently positive charged molecular ions without multiple ion adducts by MALDI-TOF-MS. The MS signal intensities of lactose, sialylated N-glycans derived from bovine fetuin and neutral N-glycans derived from RNaseB and ovalbumin were boosted by 7.44, 9.13, 12.96 and 13.47 folds on average (n = 3), respectively. More importantly, after GTOD strategy, unwanted desialylation of sialylated glycans during MS was suppressed. The detection limit of the assay is desirable since the nanogram of N-glycans derived from 0.16 μg ovalbumin could be detected. The assay demonstrated good stability (RSD≤2.95%, within 10 days), reliable reproducibility (RSD = 2.96%, n = 7) and a desirable linear dynamic range from 78 nmol/mL to 10 μmol/mL. The strategy has been successfully applied to MS analysis of reducing glycans from human milks, neutral and sialylated O-, N-glycans from glycoproteins, and reducing glycans derived from glycosphingolipids, presenting neater [M] + signals which allow detection of more low-abundance glycans and assignation of Neu5Ac vs. Neu5Gc or fucose vs. hexose in glycans due to the absence of the ambiguous interpretation from multiple peaks (ion adducts [M+Na] + and [M+K] + ). Moreover, the GTOD assay prevents desialylation during MALDI-TOF-MS profiling and enables distinct linkage-specific characterization of terminal sialic acids of N-glycans derived from human serum protein when combines with an esterification.
- Published
- 2018
31. Additional file 3: Table S1. of The invasive MED/Q Bemisia tabaci genome: a tale of gene loss and gene gain
- Author
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Xie, Wen, Yang, Xin, Chunhai Chen, Zezhong Yang, Litao Guo, Wang, Dan, Jinqun Huang, Hailin Zhang, Yanan Wen, Jinyang Zhao, Qingjun Wu, Shaoli Wang, Coates, Brad, Xuguo Zhou, and Youjun Zhang
- Abstract
Reads mapped ratio of MED/Q and MEAM1/B with each other. (DOCX 13Â kb)
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- 2018
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- View/download PDF
32. Additional file 6: Table S3. of The invasive MED/Q Bemisia tabaci genome: a tale of gene loss and gene gain
- Author
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Xie, Wen, Yang, Xin, Chunhai Chen, Zezhong Yang, Litao Guo, Wang, Dan, Jinqun Huang, Hailin Zhang, Yanan Wen, Jinyang Zhao, Qingjun Wu, Shaoli Wang, Coates, Brad, Xuguo Zhou, and Youjun Zhang
- Abstract
Identification and analysis of the orthologous genes between MED/Q and MEAM1/B. (DOCX 15Â kb)
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- 2018
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- View/download PDF
33. Additional file 27: Table S13. of The invasive MED/Q Bemisia tabaci genome: a tale of gene loss and gene gain
- Author
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Xie, Wen, Yang, Xin, Chunhai Chen, Zezhong Yang, Litao Guo, Wang, Dan, Jinqun Huang, Hailin Zhang, Yanan Wen, Jinyang Zhao, Qingjun Wu, Shaoli Wang, Coates, Brad, Xuguo Zhou, and Youjun Zhang
- Subjects
bacteria ,biochemical phenomena, metabolism, and nutrition - Abstract
Genes involved in B vitamin biosynthesis in Candidatus Hamiltonella defense. (DOCX 51Â kb)
- Published
- 2018
- Full Text
- View/download PDF
34. Additional file 18: Table S6. of The invasive MED/Q Bemisia tabaci genome: a tale of gene loss and gene gain
- Author
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Xie, Wen, Yang, Xin, Chunhai Chen, Zezhong Yang, Litao Guo, Wang, Dan, Jinqun Huang, Hailin Zhang, Yanan Wen, Jinyang Zhao, Qingjun Wu, Shaoli Wang, Coates, Brad, Xuguo Zhou, and Youjun Zhang
- Abstract
Gene ontology of PSG in MED/Q and MEAM1/B (FDR
- Published
- 2018
- Full Text
- View/download PDF
35. The invasive MED/Q Bemisia tabaci genome: a tale of gene loss and gene gain
- Author
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Zezhong Yang, Qingjun Wu, Wen Xie, Chunhai Chen, Brad S. Coates, Yanan Wen, Dan Wang, Litao Guo, Shaoli Wang, Youjun Zhang, Jinyang Zhao, Jinqun Huang, Hailin Zhang, Xuguo Zhou, and Xin Yang
- Subjects
0301 basic medicine ,Crops, Agricultural ,lcsh:QH426-470 ,lcsh:Biotechnology ,Genome, Insect ,Biology ,Genome ,Bemisia tabaci ,MED/Q ,Host Specificity ,Hemiptera ,Insecticide Resistance ,03 medical and health sciences ,Cytochrome P-450 Enzyme System ,RNA interference ,lcsh:TP248.13-248.65 ,Genetics ,Coding region ,Gene family ,Animals ,Glucuronosyltransferase ,Gene gain and loss ,Symbiosis ,Gene ,Phylogeny ,Synteny ,Invasive species ,lcsh:Genetics ,030104 developmental biology ,Detoxification enzymes ,Multigene Family ,Horizontal gene transfer ,Insect Proteins ,DNA microarray ,Transcriptome ,Biotechnology ,Research Article - Abstract
Background Sweetpotato whitefly, Bemisia tabaci MED/Q and MEAM1/B, are two economically important invasive species that cause considerable damages to agriculture crops through direct feeding and indirect vectoring of plant pathogens. Recently, a draft genome of B. tabaci MED/Q has been assembled. In this study, we focus on the genomic comparison between MED/Q and MEAM1/B, with a special interest in MED/Q’s genomic signatures that may contribute to the highly invasive nature of this emerging insect pest. Results The genomes of both species share similarity in syntenic blocks, but have significant divergence in the gene coding sequence. Expansion of cytochrome P450 monooxygenases and UDP glycosyltransferases in MED/Q and MEAM1/B genome is functionally validated for mediating insecticide resistance in MED/Q using in vivo RNAi. The amino acid biosynthesis pathways in MED/Q genome are partitioned among the host and endosymbiont genomes in a manner distinct from other hemipterans. Evidence of horizontal gene transfer to the host genome may explain their obligate relationship. Putative loss-of-function in the immune deficiency-signaling pathway due to the gene loss is a shared ancestral trait among hemipteran insects. Conclusions The expansion of detoxification genes families, such as P450s, may contribute to the development of insecticide resistance traits and a broad host range in MED/Q and MEAM1/B, and facilitate species’ invasions into intensively managed cropping systems. Numerical and compositional changes in multiple gene families (gene loss and gene gain) in the MED/Q genome sets a foundation for future hypothesis testing that will advance our understanding of adaptation, viral transmission, symbiosis, and plant-insect-pathogen tritrophic interactions. Electronic supplementary material The online version of this article (10.1186/s12864-018-4448-9) contains supplementary material, which is available to authorized users.
- Published
- 2018
36. Additional file 4: Table S2. of The invasive MED/Q Bemisia tabaci genome: a tale of gene loss and gene gain
- Author
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Xie, Wen, Yang, Xin, Chunhai Chen, Zezhong Yang, Litao Guo, Wang, Dan, Jinqun Huang, Hailin Zhang, Yanan Wen, Jinyang Zhao, Qingjun Wu, Shaoli Wang, Coates, Brad, Xuguo Zhou, and Youjun Zhang
- Abstract
Syntenic alignment between MED/Q and MEAM1/B genome. (DOCX 16Â kb)
- Published
- 2018
- Full Text
- View/download PDF
37. Constitution of a visual detection system for lead(<scp>ii</scp>) on polydiacetylene–glycine embedded nanofibrous membranes
- Author
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Tao Ke, Jianyong Yu, Jingyuan Chen, Gang Sun, Lihuan Wang, Yanan Wen, Bin Ding, and Yan Li
- Subjects
chemistry.chemical_classification ,Materials science ,Renewable Energy, Sustainability and the Environment ,Polyacrylonitrile ,Analytical chemistry ,General Chemistry ,Polymer ,Conjugated system ,Fluorescence ,Standard curve ,chemistry.chemical_compound ,Membrane ,chemistry ,Chemical engineering ,General Materials Science ,Sample preparation ,Naked eye - Abstract
With the aim to develop pH-paper-like lead ion (Pb2+) sensing materials, we demonstrate a colorimetric strip, which relies on a SiO2 nanoparticle (NP) decorated polydiacetylene embedded polyacrylonitrile nanofibrous membrane (PAN NFM), that undergoes a brilliant blue-to-red color transition as well as ‘Turn-On’ fluorescence upon incubation with Pb2+. The introduction of an amino acid (glycine) headgroup into a supramolecularly assembled 10,12-pentacosadiynoic acid results in a Pb2+-induced chromic conjugated polymer that is quickly photopolymerizable, rapidly responsive to Pb2+ and specifically identifiable. Moreover, the SiO2 NPs are certified as an efficient sensitivity promoter when their content reaches 0.5 wt% in the strip. With this understanding, the color change that is caused by 0.24 μM Pb2+ at ambient temperature can be easily perceived by the naked eye, which indicates the superiority of our strip compared with previous approaches, and it is also noteworthy that a fluorescent signal could also be produced in 2 min. Furthermore, RGB (red-green-blue) digital parameters obtained from images of the strips and automatic read outs via a smartphone were processed statistically through principal component analysis and then used to elaborate the standard curve and quantify Pb2+ concentration. This methodology for assaying and quantifying Pb2+ avoids time-consuming sample preparation, expensive laboratory techniques, and specialized personnel required to carry out conventional analytical methods.
- Published
- 2015
38. Genome sequencing of the sweetpotato whitefly Bemsia tabaci MED/Q
- Author
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Yuzhou Du, Yong Liu, Youjun Zhang, Chunhai Chen, Ye Yin, Ming Chen, Xin Yang, Brad S. Coates, Murad Ghanim, Huipeng Pan, Xiaoguo Jiao, Hongying Cui, Judith K. Brown, Evan L. Preisser, Jinquan Xia, Yanan Wen, Qingjun Wu, Dong Chu, Xinqiu Tan, Fengming Yan, Feng Ge, Baoyun Xu, Shaoli Wang, Zezhong Yang, Xueping Zhou, Yang Zeng, Bao-Li Qiu, Helene Delatte, Litao Guo, Yating Liu, Lixia Tian, Xiao-Wei Wang, Dan Wang, Jixing Xia, Chen Luo, Wen Xie, Xuguo 'Joe' Zhou, Qiang Gao, Midatharahally N. Maruthi, Jinyang Zhao, Jinqun Huang, Xianchun Li, and Fang-Hao Wan
- Subjects
0106 biological sciences ,0301 basic medicine ,Male ,Phylogénie ,Assembly ,Genome, Insect ,01 natural sciences ,Bemisia tabaci ,Beijing ,Ipomoea batatas ,biology ,food and beverages ,Genomics ,Computer Science Applications ,Female ,Séquence nucléotidique ,Research system ,Whitefly Bemisia tabaci ,Annotation ,Health Informatics ,Whitefly ,Data Note ,DNA sequencing ,génomique ,Hemiptera ,03 medical and health sciences ,Botany ,Animals ,China ,SB ,Gene Library ,Génie génétique ,Génome ,business.industry ,Pest control ,Sequence Analysis, DNA ,biology.organism_classification ,H10 - Ravageurs des plantes ,Biotechnology ,030104 developmental biology ,Agriculture ,business ,010606 plant biology & botany - Abstract
The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future ‘pan-genomic’ comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management.
- Published
- 2017
39. Competition between surface trapping and nonradiative energy transfer to gold nanofilm.
- Author
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Zhenling Yang, Yuqiang Liu, Xing He, Yanan Wen, and Yanqiang Yang
- Subjects
ENERGY transfer ,QUANTUM dots ,PHOTOLUMINESCENCE ,METALS ,PICOSECOND pulses - Abstract
Nonradiative resonant energy transfer from CdSeS quantum dot to gold nanofilm was investigated by taking nanosecond and picosecond time resolved photoluminescence measurements. Surface plasma resonant absorption peak of gold nanofilm was adjusted to meet the near resonant conditions with the fluorescence peak of quantum dot by changing the thickness. Surface trapping state was proved to be the origin of the long lifetime component by comparing fresh and eight months aged quantum dot. It was observed that the excitonic state lifetime of the quantum dots was reduced by nonradiative resonant energy transfer to gold nanofilm. Nonradiative resonant energy transfer time, which was comparable with the surface trapping time, was calculated based on the data of picosecond photoluminescence measurements. No nonradiative energy transfer from surface trapping state to gold nanofilm, thus the lifetime of surface trapping state was not affected obviously. It is suggested that in the assembly combined with quantum dot and gold nanostructure, nonradiative energy transfer will occur after the population of excitonic state, and compete with surface trapping process. The interactions between surface trapping state and gold nanoflim were not exhibited. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
40. [Anti-oncogene of opioid binding protein/cell adhesion molecule-like methylation status in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate]
- Author
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Hongmei, Liu, Quankai, Wang, Guangyun, Xie, Huanhuan, Li, Anna, Wang, Yanan, Wen, and Jianning, Xu
- Subjects
Cell Transformation, Neoplastic ,Epoxy Compounds ,Humans ,Methacrylates ,Epithelial Cells ,Genes, Tumor Suppressor ,DNA Methylation ,GPI-Linked Proteins ,Promoter Regions, Genetic ,Cell Adhesion Molecules ,Cells, Cultured - Abstract
To analyze the opioid binding protein/cell adhesion molecule-like (OPCML) methylation status at different stages of malignant transformation of human bronchial epithelial (16HBE) cells induced by Glycidyl Methacrylate (GMA) and to explore the effect of OPCML methylation in the process of malignant transformation.Cells were harvested at different stages (the 10th generation, the 20th generation and the 30th generation). To verify the Methylation chip result of OPCML methylation status in the process of malignant transformation, we detected it by methylation-specific-PCR (MSP); Real-time fluorescence Quantitative PCR (qPCR) were applied to measure the gene expression levels of OPCML at different transformed stage, and compared with the control groups (treated with DMSO).Based on the result of methylation chip, the gene of OPCML methylation occurred in all stages, which was consistent to the result of MSP; qPCR showed that the levels of gene expression decreased in the 20th generation and 30th generation.Methylation status of OPCML gene promoter could be considered as a stable and specific biomarker in the transformation process.
- Published
- 2016
41. Ulcerate colitis and protein C system: is there a link of causality
- Author
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Guanchang Cheng, Yan-Min Li, Xue-Qun Ren, Yu-Xia Li, Hui-Chao Wang, Yanan Wen, Xu-Hong Lin, Dan-Dan Wei, and Zhaoya Yu
- Subjects
Protein C inhibitor ,CD14 ,Inflammation ,Thrombomodulin ,Inflammatory bowel disease ,Proinflammatory cytokine ,03 medical and health sciences ,Mice ,0302 clinical medicine ,medicine ,Animals ,Humans ,Letter to the Editor ,Blood Coagulation ,business.industry ,Gastroenterology ,medicine.disease ,Endothelial stem cell ,030220 oncology & carcinogenesis ,Immunology ,Cancer research ,030211 gastroenterology & hepatology ,Colitis, Ulcerative ,medicine.symptom ,business ,Protein C ,medicine.drug - Abstract
Dear Editor: Ulcerate colitis (UC), the main type of inflammatory bowel disease (IBD), is an unexplained immune-mediated intestinal mucosal inflammation with higher cancer risk. Because of an interaction between inflammation and coagulation in the disease progression, hypercoagulative state is often seen in patients of UC. Endothelial cell damage plays an important role in the activation of coagulation system in patients with UC, while protein C (PC) system, a well-characterized anticoagulant system located on the vascular endothelial cell surface, which consists of protein S (PS), endothelial cell protein C receptor (EPCR), PC, protein C inhibitor, and thrombomodulin (TM), is a mediator of endothelial function. Recent studies indicated that PC system plays an important role in regulating intestinal homeostasis both in human intestinal microvascular endothelial cells and animal models of colitis. Therefore, we speculate that the imbalance of PC system may be one of the pathogenesis of UC patients. Our in vivo study verified that the degree of inflammation was negatively correlated with the PC activity, and mouse colonic macrophages and mucosa microvascular endothelial cells are suggested as the major mediators involved. Until today, it is unclear why PC system changed, and how macrophages and mucosa microvascular endothelial cells interact with each other in UC pathology, so it is important to investigate the changes and mechanisms of PC system, and to find new drug target. Further research in vitro revealed that TNF-α and IL-6 levels were increased in the supernatant of macrophages from dextran sulfate sodium (DSS) mice colonic tissue, and these conditions were only seen in CD14+CD64+ subtype macrophages. In addition, after incubation of TNF-α or IL-6 with colonic mucosal microvascular endothelial cells, the activity of PC system was inhibited, manifested as decreased APC activity, and downregulated expression of EPCR. Furthermore, β-arrestin-pJNK MAPK expressions were upregulated by real-time PCR and Western blot. Taken together into account, due to complex UC mechanism, its pathogenesis is unclear, and there is little research about colonic mucosal microvascular, especially the study for PC system changes in the pathogenesis of UC is rare. In our study, we found that in DSS-induced mouse colitis, colonic CD14+CD64+ macrophages produced more inflammatory cytokines, which result in inhibited PC pathway via mucosal microvascular endothelial cells, and β-arrestin-pJNK MAPK signal pathway may involved. Therefore, enhancing the activity of PC system provides a major theoretical and clinical significance to find new drug target for the treatment of UC.
- Published
- 2015
42. [Determination of 2, 4-dichlorophenoxyacetic acid in air of workplace by high-performance liquid chromatography]
- Author
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Yanan, Wen, Zhaohui, Fu, Jianning, Xu, Shichuan, Tang, Quankai, Wang, Huanhuan, Li, Guangyun, Xie, Yuling, Zhu, Yiting, Gu, and Feng, Tan
- Subjects
Air ,Air Pollutants, Occupational ,2,4-Dichlorophenoxyacetic Acid ,Workplace ,Chromatography, High Pressure Liquid - Abstract
To develop a method for determination of 2, 4-dichlorophenoxyacetic acid (2, 4-D) in the air of workplace by high-performance liquid chromatography.2, 4-D was collected by ultrafine glass filters, desorbed by methanol, separated by a C18 column, and detected by a UV detector. Identification and quantification of 2, 4-D were performed by retention time and peak areas, respectively.The linear range of the test was 2∼200 µg/ml; the elution efficiency was 94.6%- 95.9%; the limit of detection (S/N = 3) was 0.034 µg/ml (injection volume of 20 µl eluant); the lower limit of quantification (S/N = 10) was 0.11 µg/ml; the minimum detectable concentration was 0.011 mg/m(3); the minimum quantifiable concentration was 0.037 mg/m(3) (with sampled air volume of 45 L).This method is convenient and simple in sample collection and preparation, and satisfies all methodological requirements. Therefore, this method is useful for the determination of 2, 4-D in the air of workplace.
- Published
- 2014
43. New approach for underwater imaging and processing
- Author
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Guozun Zhou, Yanan Wen, Weijian Tian, Bing Zheng, Qiong Wu, and Hui Dong
- Subjects
Channel (digital image) ,Noise (signal processing) ,Scattering ,business.industry ,Computer science ,media_common.quotation_subject ,Monte Carlo method ,Image processing ,Image (mathematics) ,Contrast (vision) ,Computer vision ,Artificial intelligence ,Underwater ,Visibility ,business ,Physics::Atmospheric and Oceanic Physics ,media_common - Abstract
Due to the absorptive and scattering nature of water, the characteristic of underwater image is different with it in the air. Underwater image is characterized by their poor visibility and noise. Getting clear original image and image processing are two important problems to be solved in underwater clear vision area. In this paper a new approach technology is presented to solve these problems. Firstly, an inhomogeneous illumination method is developed to get the clear original image. Normal illumination image system and inhomogeneous illumination image system are used to capture the image in same distance. The result shows that the contrast and definition of processed image is get great improvement by inhomogeneous illumination method. Secondly, based on the theory of photon transmitted in the water and the particularity of underwater target detecting, the characters of laser scattering on underwater target surface and spatial and temporal characters of oceanic optical channel have been studied. Based on the Monte Carlo simulation, we studied how the parameters of water quality and other systemic parameters affect the light transmitting through water at spatial and temporal region and provided the theoretical sustentation of enhancing the SNR and operational distance.
- Published
- 2014
44. SORL1 is genetically associated with neuropathologically characterized late-onset Alzheimer's disease
- Author
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Nobutaka Kitamura, Akiyoshi Kakita, Hiroyuki Hatsuta, Hiroyasu Akatsu, Akinori Miyashita, Hitoshi Takahashi, Yasuo Ihara, Shigeo Murayama, Tamao Tsukie, Takayuki Yamamoto, Yuko Saito, Kenji Kosaka, Kohei Akazawa, Yanan Wen, Ryozo Kuwano, and Haruyasu Yamaguchi
- Subjects
Male ,Linkage disequilibrium ,Genotype ,SORL1 ,Single-nucleotide polymorphism ,Genome-wide association study ,Disease ,Biology ,Polymorphism, Single Nucleotide ,Linkage Disequilibrium ,Cohort Studies ,Apolipoproteins E ,Asian People ,Alzheimer Disease ,Databases, Genetic ,SNP ,Humans ,Age of Onset ,Gene ,Alleles ,LDL-Receptor Related Proteins ,Aged ,Genetics ,Aged, 80 and over ,General Neuroscience ,Haplotype ,Brain ,Chromosome Mapping ,Membrane Transport Proteins ,General Medicine ,Middle Aged ,Psychiatry and Mental health ,Clinical Psychology ,Logistic Models ,Data Interpretation, Statistical ,Female ,Geriatrics and Gerontology ,Genome-Wide Association Study - Abstract
SORL1 was shown to be genetically associated with late-onset Alzheimer's disease (LOAD) in a large-scale genome- wide association study (GWAS) involving clinically verified subjects. Here, we attempted to replicate the association of SORL1 in Japanese neuropathologically characterized brain donor subjects (LOAD, 213; control, 370) through a single-nucleotide polymorphism (SNP)-based genetic study involving 19 SNPs: 11 SNPs were selected from the initial study reported by Rogaeva et al. (2007), and the other eight were from our GWAS. Among these SNPs, five exhibited a significant association with LOAD after multiple test correction (p < 2.63E-03 (= 0.05/19)), which was supported by means of multiple logistic regression analysis with adjustment for age, gender, and carrier status of the APOE4 allele. Three of these SNPs (rs985421, rs12364988 (Rogaeva's SNP 7), and rs4598682) were encompassed by a 5' linkage disequilibrium (LD) region, and the remaining two (rs3781834 and rs3781836) by a 3' LD region. Strong LD among SNPs was observed within each LD region, implying that there are two genomic regions showing association with LOAD in SORL1. Case-control haplotype analysis demonstrated that some haplotypes are associated with LOAD in both LD regions. Our replication study strongly supports the preceding evidence that SORL1 is likely one of the genes associated with LOAD.
- Published
- 2013
45. Influence of Laser Incident Energy on Chemical Composition, Crystal Structure, Morphology and Band Gap of Cu2ZnSnS4 Thin Films by Pulsed Laser Deposition
- Author
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Yan Dong, Lin Li, Qi Liang, Min Yao, and Yanan Wen
- Subjects
Materials science ,Absorption spectroscopy ,Band gap ,business.industry ,Substrate (electronics) ,Laser ,Pulsed laser deposition ,law.invention ,chemistry.chemical_compound ,Optics ,chemistry ,law ,Optoelectronics ,CZTS ,Thin film ,business ,Spectroscopy - Abstract
The Cu2ZnSnS4 (CZTS) thin films were successfully prepared on glass substrate by pulsed laser deposition (PLD) using CZTS target. The laser incident energy was varied from 3 J·cm -2 to 6 J·cm -2 at the interval of 1 J·cm -2 , and its influence on chemical composition, crystal structure, morphology and band gap of CZTS thin films was investigated by energy dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), atomic force microscopy (AFM) and ultraviolet-visible-near infrared (UV-Vis-NIR) absorption spectra, respectively. The result of EDS indicated that these CZTS thin films were Cu-rich and S-poor. The XRD study showed CZTS thin films exhibited strong preferential orientation of grains along (112) direction. The band gap of CZTS thin films was 1.72, 1.37, 1.25 and 1.11 eV corresponding to incident laser energy of 3, 4, 5 and 6 J·cm -2 .
- Published
- 2012
46. Indirect activation of porphyrin by semiconductor quantum dot with two-photon excitation
- Author
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Yanan Wen and Yanqiang Yang
- Subjects
Photon ,Condensed Matter::Other ,Chemistry ,Physics::Optics ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,Photochemistry ,Molecular physics ,Fluorescence ,Condensed Matter::Materials Science ,Förster resonance energy transfer ,Two-photon excitation microscopy ,Quantum dot laser ,Quantum dot ,Femtosecond ,Spectroscopy - Abstract
Flourescence resonance energy transfer (FRET) between CdSe quantum dots (QDs) and mesotetraphenylporphyrin (TPP) photosensitizers is studied by two-photon excitation fluorescent spectroscopy with 800nm femtosecond laser pulses. Evident transfer results are obtained in both the steady-state and time-resolved experiments. A slight peak shift to the red region in the emission of the CdSe QDs is observed after addition of TPP, which is speculated to be caused by energy transfer from QDs of small size to larger. An efficiency of about 0.3 accounts for the non-radiative energy transfer is estimated with the time-resolved method.
- Published
- 2009
47. Genome sequencing of the sweetpotato whitefly Bemisia tabaci MED/Q.
- Author
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Wen Xie, Chunhai Chen, Zezhong Yang, Litao Guo, Xin Yang, Dan Wang, Ming Chen, Jinqun Huang, Yanan Wen, Yang Zeng, Yating Liu, Jixing Xia, Lixia Tian, Hongying Cui, Qingjun Wu, Shaoli Wang, Baoyun Xu, Xianchun Li, Xinqiu Tan, and Ghanim, Murad
- Subjects
NUCLEOTIDE sequencing ,SWEETPOTATO whitefly - Abstract
The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future 'pan-genomic' comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
48. Indirect activation of porphyrin by semiconductor quantum dots with two-photon excitation
- Author
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Yanan Wen
- Subjects
Mechanical Engineering ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials - Published
- 2010
49. Underwater image color correct in extremely poor visibility.
- Author
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Beilei Hu, Bing Zheng, Yu Yang, and Yanan Wen
- Published
- 2013
50. Impact of noble metal nanostructures on surface trapping state of semiconductor quantum dots.
- Author
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Zhenling Yang, Yanan Wen, Qingkun Meng, Yuqiang Liu, Yunfei Song, Xing He, Aihua Li, Guoyang Yu, Yanqiang Yang, and Wenzhi Wu
- Subjects
- *
NANOSTRUCTURES , *TRAPPED-particle instabilities , *QUANTUM dots , *QUANTUM electronics , *SEMICONDUCTORS , *PRECIOUS metals - Abstract
The impact of noble metal nanostructures on the surface trapping state of semiconductor quantum dots was investigated by taking nanosecond time resolved photoluminescence measurements. It was observed that nonradiative resonant energy transfer from the excitonic state of quantum dots to Au nanofilm reduced the lifetime and intensity of excitonic state emission. No nonradiative resonant energy transfer from surface trapping state to Au nanofilm was observed. It is possibly implied that noble metal nanostructures have no impact on the surface trapping state of semiconductor quantum dots. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
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