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Glutathione S-transferases are involved in thiamethoxam resistance in the field whitefly Bemisia tabaci Q (Hemiptera: Aleyrodidae)
- Source :
- Pesticide Biochemistry and Physiology. 134:73-78
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The whitefly, Bemisia tabaci, has developed a high level of resistance to thiamethoxam, a second generation neonicotinoid insecticide that has been widely used to control this pest. In this study, we assessed the level of cross-resistance, the activities of detoxifying enzymes, and the expression profiles of 23 glutathione S-transferase (GST) genes in a thiamethoxam-resistant ant and -susceptible strain of Bemisia tabaci Q. The thiamethoxam-resistant strain showed a moderate level of cross-resistance to another nicotinoid insecticide imidacloprid, a low level of cross-resistance to acetamiprid and nitenpyram, and no significant cross-resistance to abamectin and bifenthrin. Among detoxifying enzymes, only GSTs had significantly higher activity in the resistant strain than in the susceptible strain. Seven of 23 GST genes were over-expressed in the resistant strain relative to the susceptible strain. Using the technology of RNA interference to knockdown a GST gene (GST14), the results showed that silencing GST14 increased the mortality of whiteflies to thiamethoxam in Bemisia tabaci.
- Subjects :
- Male
0106 biological sciences
0301 basic medicine
Insecticides
Pesticide resistance
Health, Toxicology and Mutagenesis
Bifenthrin
Gene Expression
Whitefly
01 natural sciences
Acetamiprid
Carboxylesterase
Microbiology
Hemiptera
Insecticide Resistance
Neonicotinoids
03 medical and health sciences
chemistry.chemical_compound
Cytochrome P-450 Enzyme System
Imidacloprid
Oxazines
Botany
Animals
Glutathione Transferase
Nitenpyram
biology
General Medicine
Nitro Compounds
biology.organism_classification
Thiazoles
010602 entomology
030104 developmental biology
chemistry
Abamectin
Insect Proteins
Female
RNA Interference
Thiamethoxam
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 00483575
- Volume :
- 134
- Database :
- OpenAIRE
- Journal :
- Pesticide Biochemistry and Physiology
- Accession number :
- edsair.doi.dedup.....8753dc7e2f76edab2d4181e97403da2b