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Glutathione S-transferases are involved in thiamethoxam resistance in the field whitefly Bemisia tabaci Q (Hemiptera: Aleyrodidae)

Authors :
Litao Guo
Youjun Zhang
Yanan Wen
Jixing Xia
Xiaomao Zhou
Fengshan Yang
Yating Liu
Chao He
Xin Yang
Qingjun Wu
Ze-Zong Yang
Shaoli Wang
Wen Xie
Source :
Pesticide Biochemistry and Physiology. 134:73-78
Publication Year :
2016
Publisher :
Elsevier BV, 2016.

Abstract

The whitefly, Bemisia tabaci, has developed a high level of resistance to thiamethoxam, a second generation neonicotinoid insecticide that has been widely used to control this pest. In this study, we assessed the level of cross-resistance, the activities of detoxifying enzymes, and the expression profiles of 23 glutathione S-transferase (GST) genes in a thiamethoxam-resistant ant and -susceptible strain of Bemisia tabaci Q. The thiamethoxam-resistant strain showed a moderate level of cross-resistance to another nicotinoid insecticide imidacloprid, a low level of cross-resistance to acetamiprid and nitenpyram, and no significant cross-resistance to abamectin and bifenthrin. Among detoxifying enzymes, only GSTs had significantly higher activity in the resistant strain than in the susceptible strain. Seven of 23 GST genes were over-expressed in the resistant strain relative to the susceptible strain. Using the technology of RNA interference to knockdown a GST gene (GST14), the results showed that silencing GST14 increased the mortality of whiteflies to thiamethoxam in Bemisia tabaci.

Details

ISSN :
00483575
Volume :
134
Database :
OpenAIRE
Journal :
Pesticide Biochemistry and Physiology
Accession number :
edsair.doi.dedup.....8753dc7e2f76edab2d4181e97403da2b