1. Antibody detection of SARS-CoV spike and nucleocapsid protein
- Author
-
Shin-Tsung Ho, Jien-Ming Chu, Tsai-Yin Wei, Chia-Ju Chen, Yun-Ting Hsu, Chao-Chih Wu, Yuh-Cheng Yang, Ching-Hsin Chen, Yen-Ta Lu, Po-Chen Chu, Chia-Chien Hung, Mau-Sun Chang, Ya-Lin Jan, and Chi-Chen Fan
- Subjects
viruses ,Blotting, Western ,Molecular Sequence Data ,Biophysics ,Fluorescent Antibody Technique ,Spike ,medicine.disease_cause ,Biochemistry ,Virus ,Article ,law.invention ,Western blot ,Viral Envelope Proteins ,law ,medicine ,Humans ,Amino Acid Sequence ,skin and connective tissue diseases ,Nucleocapsid ,Molecular Biology ,Peptide sequence ,Coronavirus ,SARS ,Membrane Glycoproteins ,biology ,medicine.diagnostic_test ,fungi ,Cell Biology ,Nucleocapsid Proteins ,Virology ,respiratory tract diseases ,Blot ,body regions ,Severe acute respiratory syndrome-related coronavirus ,Polyclonal antibodies ,Spike Glycoprotein, Coronavirus ,biology.protein ,Recombinant DNA ,Antibody ,Subcellular Fractions - Abstract
Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV.
- Published
- 2004