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Antibody detection of SARS-CoV spike and nucleocapsid protein
- Source :
- Biochemical and Biophysical Research Communications
- Publication Year :
- 2004
-
Abstract
- Early detection and identification of SARS-CoV-infected patients and actions to prevent transmission are absolutely critical to prevent another SARS outbreak. Antibodies that specifically recognize the SARS-CoV spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. For this reason, we raised peptide-induced polyclonal antibodies against SARS-CoV spike protein and polyclonal antibodies against SARS-CoV nucleocapsid protein using 6x His nucleocapsid recombinant protein. Western blot analysis and immunofluorescent staining showed that these antibodies specifically recognized SARS-CoV.
- Subjects :
- viruses
Blotting, Western
Molecular Sequence Data
Biophysics
Fluorescent Antibody Technique
Spike
medicine.disease_cause
Biochemistry
Virus
Article
law.invention
Western blot
Viral Envelope Proteins
law
medicine
Humans
Amino Acid Sequence
skin and connective tissue diseases
Nucleocapsid
Molecular Biology
Peptide sequence
Coronavirus
SARS
Membrane Glycoproteins
biology
medicine.diagnostic_test
fungi
Cell Biology
Nucleocapsid Proteins
Virology
respiratory tract diseases
Blot
body regions
Severe acute respiratory syndrome-related coronavirus
Polyclonal antibodies
Spike Glycoprotein, Coronavirus
biology.protein
Recombinant DNA
Antibody
Subcellular Fractions
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 314
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....a47f654a2f1197ea9e54406085d4dde9