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A novel fluorescent probe L consisting of β-Cyclodextrin (β-CD) and thiosemicarbazide moieties was designed for copper ions detection. Apparent fluorescence quenching of probe L in Tris-HCl（pH = 7.4）solution was observed only in the presence of Cu

Tourism destination system can be considered as a complex nonlinear dynamic system. It’s difficult to accurately evaluate the operational orderliness with existing evaluation methods due to the complexity and dynamics of system. This paper attempts to introduce the concept of entropy in thermodynamics into organizational management, hoping to establish a nonlinear dynamic method for assessing destination system orderliness from management lens. Combining information entropy formula and the characteristics of tourism destination, this paper analyzed the entropy flow and negative entropy flow in the destination business ecosystem. Then an entropy evaluation index was constructed and the weight of each specific primary indicator and secondary indicator were calculated with the combination of analytic hierarchy process (AHP) and principal component analysis (PCA). Finally, taking JIUZHAI Valley as an example, this paper tested and modified the nonlinear dynamic evaluation approach. The nonlinear dynamic evaluation approach not only emphasize the overall operation state and process of destination system, but also take into account the nonlinear relationships amongst the internal elements of destination, contributing to overcoming the defects and deficiencies of traditional evaluation methods.

With the acceleration development of urbanization, the infrastructure construction plays an increasingly crucial role in the expansion process of cities. However, the existing decision-making methods of urban infrastructure investment still stay at the static level, resulting in a huge waste of resources, which does not meet the needs of dynamic urban development and management. Based on geometric Brownian motion, option function and the theory of real option, this paper establishes a dynamic differential optimal investment opportunity decision model applied to urban infrastructure construction. An example was then conducted to test the operability and application of the proposed model. The results confirm that the proposed approach contributes to improving the scientificity of urban infrastructure planning and construction, and realizing the maximization of infrastructure’s social value. Meanwhile, this paper contributes to promoting the development of city infrastructure investment decision-making methods from static to dynamic, and from partial to overall.

With the rapid development of tourism economics, personalized and diversified demand of tourists and the advancement of modern information technologies, the investment of scenic spots faces multitudinous uncertainties and emergencies and can be considered as a nonlinear dynamic system. Given this situation, the existing investment decision-making model (e.g. net present value, NPV) is difficult to estimate the realistic value of scenic spots, and new theory and decision-making model are needed in the area. Based on real option theory and option function, the paper establishes a nonlinear dynamic investment decision model of scenic spots with uncertainties and emergencies, and then, the model is verified with an example. The results show that with the impacts of emergencies, when the investment value is considered, the option value of the investment project in the scenic spot can be maximized, thus maximizing the total value of the investment project. The proposed nonlinear dynamic investment decision model proves that the favourable impact of emergencies on the investment of scenic spots increases the investment opportunity value of scenic projects, thus increasing the investment value of scenic projects, emphasizes more on the dynamic nature of investment and the favourable conditions in the face of crisis, and helps enterprises investing in scenic spots make full use of its uncertain environment and the favourable impact of emergencies to choose the optimal investment opportunity. Therefore, this study has both theoretical and practical significance.

With the development of biomass power generation technology, biomasswaste has a more excellent recycling value. The article establishes a biomasswaste inventory model based on the material flow analysis method andpredicts raw material waste’s energy utilization potential. The results showthat the amount of biomass waste generated from 2016 to 2020 is on therise. In 2020, biomass waste’s energy utilization can reach 107,802,300 tons,equivalent to 1,955.28PJ of energy. Through biomass energy analysis andemission analysis, the results show that the biomass waste can generate182.02 billion kW·h in 2020, which can replace 35.9% of the region’s totalpower consumption, which is compared with the traditional power generationmethod under the same power generation capacity. Power generation can reduce SO2 emissions by 250,400 tons, NOx emissions by 399,300 tons,and PM10 emissions by 49,700 tons. Reduce direct economic losses by712 million yuan. Therefore, Chinese promotion of the recycling of biomasswaste and the acceleration of the biomass energy industry’s development isof great significance for reducing pollutant emissions and alleviating energypressure.

In the research of intelligent grids, people usually pay attention to the interaction between power source and load to absorb more new energy. In contrast, the utilization of power source and load on grid resources is less considered, and energy-grid-load is not fully explored. The profound benefits and value of the three interactions. To this end, the paper proposes the interactive mode and method of power supply, power grid, and load based on full-cost electricity price. The power source side needs to consider the impact of transmission costs when optimizing economic dispatch; the load side needs to calculate the full-cost electricity price based on the power flow tracking method, clarify the load’s use of power and grid resources, and reflect the “who benefits, who bears the cost” Principles and introduce demand-side response to reflect the meaning and value of full-cost electricity prices. The research found that the calculation example analysis based on the IEEE30-node system verifies that the method in this paper can reasonably allocate the cost of the power grid according to the user’s utilization of power grid resources, thereby improving the efficiency, fairness, orderliness and safety of power grid investment and operation.

To study the relationship between HPLC characteristic spectrum and pharmacodynamics on anti-myocardial ischemia of GualouXiebai dropping pills.HPLC characteristic spectrum of GualouXiebai dropping pills was established, dropping pills were divided into five dose groups (3.75, 11.25, 22.5, 33.75 and 45 g/kg, equivalent to the crude herb g/kg), the mice were orally administered dropping pills once daily for 7 d, 90 min after the mice were given by intraperitoneal injection of isoprenaline to establish myocardial ischemia models, the level of CK in blood plasma were detected; Then, the correlation between characteristic spectrum and biochemical index CK was studied by grey relational analysis method.The correlation between each common peak and CK had gradually increased with the dose increased from 3.73 g/kg to 33.75 g/kg, but when the dose reached to 45 g/kg, the correlation between each common peak and CK had decreased. The variation trends of correlation of spectrum-effect relationship for different dose were similar,but the correlation variation trend of the efficacy on the No. 8 peak in 33.75 g/kg group with the other four groups in the opposite, the change trends of the No. 11 peak in 22.5 g/kg group, the No. 24 peak in 33. 75 g/kg group and the No. 37 peak in 45 g/ kg group with 3.75 g/kg group and 11.25 g/kg group on the contrary. The relational orders of spectrum-effect relationship were not consistent, respectively( the first 15 peaks) :1137243082121613201512197;11373082124211632712222010; 830122127312216123109204; 1227213122121693104173020; 830122273122161239102017.Anti-myocardial ischemia effect of GualouXiebai dropping pills comes from the synergistic or antagonistic effect among various active ingredients related to the dose. With the difference of the dosage, the relational orders of chemical components to play the role is not the same, but the main components to play a pharmacodynamic of five dose groups are consistent,the existence of the component groups lay a foundation for further study of GualouXiebai dropping pills.

Hyperlipidemia is one kind of common and frequently occurring syndromes and closely related to cardiovascular and cerebrovascular disease occurrences in the world. It is significant to explore the marine foods and potential drug resources for the application in treating the hyperlipidemia. We conducted this study to investigate the reducing effect and mechanism of kelp (Saccharina japonica) on serum lipid of rat models with hyperlipidemia induced by a fat-rich diet. The levels of serum lipid including the triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were detected by biochemical assay. Enzyme-linked immunosorbent assay was applied to determine the level of oxidized LDL (ox-LDL). The activity of lipoprotein lipase (LPL) and hepatic lipase (HL) was determined by chemical colorimetry. The concentrations of malondialdehyde (MDA) and nitric oxide (NO) were respectively measured by thiobarbituric acid assay and nitrate reductase assay. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were respectively determined by xanthinoxidase assay and chemical colorimetry. The results showed that the serum levels of TG, TC and LDL decreased, while HDL increased significantly in treated group compared with those of model groups (P

The aim of this study was to explore the effect of picroside II on neuronal apoptosis and the expression of caspase-3 and poly ADP-ribose polymerase (PARP) following middle cerebral artery occlusion/reperfusion in male Wistar rats. Picroside II (10 mg/kg) was administered intravenously into the tail vein of the animals. The neurological function deficits were evaluated with the Bederson’s test and the cerebral infarction volume was visualized with tetrazolium chloride (TTC) staining. The apoptotic cells were counted by in situ terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) assay. The immunohistochemistry stain and enzyme linked immunosorbent assay (ELISA) was used to determine the expressions of caspase-3 and PARP in brain tissue. The results indicated that rats in the control group showed neurological function deficit and cerebral infarction in ischemic hemisphere after two hours ischemia followed by 22 hours reperfusion. Caspase-3 and PARP expressions were also profound in the cortex, the striatum and the hippocampus, along with increased apoptotic cells in this group. Bederson's score, infarction volume, and expressions of caspase-3 and PARP, as well as apoptosis in the treatment group were, however, significantly decreased compared to those in the control group indicating that intravenous treatment with picroside II might be beneficial to inhibit neuronal apoptosis and, thus, to improve the neurological function of rats upon cerebral ischemia reperfusion injury.

Canola (Brassica napus) is one of several important oil-producing crops, and the physiological processes, enzymes, and genes involved in oil synthesis in canola seeds have been well characterized. However, relatively little is known about the dynamic metabolic changes that occur during oil accumulation in seeds, as well as the mechanistic origins of metabolic changes. To explore the metabolic changes that occur during oil accumulation, we isolated metabolites from both seed and silique wall and identified and characterized them by using gas chromatography coupled with mass spectrometry (GC-MS). The results showed that a total of 443 metabolites were identified from four developmental stages. Dozens of these metabolites were differentially expressed during seed ripening, including 20 known to be involved in seed development. To investigate the contribution of tissue-specific carbon sources to the biosynthesis of these metabolites, we examined the metabolic changes of silique walls and seeds under three treatments: leaf-detachment (Ld), phloem-peeling (Pe), and selective silique darkening (Sd). Our study demonstrated that the oil content was independent of leaf photosynthesis and phloem transport during oil accumulation, but required the metabolic influx from the silique wall. Notably, Sd treatment resulted in seed senescence, which eventually led to a severe reduction of the oil content. Sd treatment also caused a significant accumulation of fatty acids (FA), organic acids and amino acids. Furthermore, an unexpected accumulation of sugar derivatives and organic acid was observed in the Pe- and Sd-treated seeds. Consistent with this, the expression of a subset of genes involved in FA metabolism, sugar and oil storage was significantly altered in Pe and Sd treated seeds. Taken together, our studies suggest the metabolite profiles of canola seeds dynamically varied during the course of oil accumulation, which may provide a new insight into the mechanisms of the oil accumulation at the metabolite level.