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Introduction: Cytochrome P450 enzymes (P450s) are recognized as the most versatile catalysts worldwide, playing vital roles in numerous biological metabolism and biosynthesis processes across all kingdoms of life. Despite the vast number of P450 genes available in databases (over 300,000), only a small fraction of them (less than 0.2 %) have undergone functional characterization. Objectives: To provide a convenient platform with abundant information on P450s and their corresponding reactions, we introduce the P450Rdb database, a manually curated resource compiles literature-supported reactions catalyzed by P450s. Methods: All the P450s and Reactions were manually curated from the literature and known databases. Subsequently, the P450 reactions organized and categorized according to their chemical reaction type and site. The website was developed using HTML and PHP languages, with the MySQL server utilized for data storage. Results: The current version of P450Rdb catalogs over 1,600 reactions, involving more than 590 P450s across a diverse range of over 200 species. Additionally, it offers a user-friendly interface with comprehensive information, enabling easy querying, browsing, and analysis of P450s and their corresponding reactions. P450Rdb is free available at http://www.cellknowledge.com.cn/p450rdb/. Conclusions: We believe that this database will significantly promote structural and functional research on P450s, thereby fostering advancements in the fields of natural product synthesis, pharmaceutical engineering, biotechnological applications, agricultural and crop improvement, and the chemical industry.

Abstract Background Understanding the release characteristics of soil colloids is a prerequisite for studying the co-transport of colloids and pollutants in subsurface environment. As a crucial agricultural management measure, fertilization not only alters the material composition of farmland soil, but also significantly regulates the properties and release patterns of soil colloids. This study systematically investigated the regulatory mechanism of short-term cattle manure fertilization on the macroscopic release and microscopic properties of soil colloids with different particle sizes, providing a theoretical foundation for subsequent research on the fate and transport of agricultural non-point source pollutants. Results The colloids in natural agricultural soil primarily consist of inorganic components. Graded extraction of the colloids has revealed that the combined proportion of colloids with particle sizes of 1–2 μm and 0.45–1 μm accounts for approximately 80.5%. Applying cattle manure inhibits the release of soil colloids, and the content of large particle size (1–2 μm) components increases. The content of organic colloids is increased due to the high total organic carbon (TOC) in cattle manure, particularly those with a particle size less than 1 μm. The characterization of organic colloid components revealed a significant increase in aromatic carbon and oxygen-containing functional groups, while the aliphatic content decreased. The response sequence regarding changes in functional groups within organic colloids induced by fertilization was as follows: –CH3, –CH2 > C–O > –OH > C=C. Fertilization promotes the release of 1:1-type inorganic mineral colloids, increasing the content of poorly crystalline minerals. The retention of aromatic carbon and oxygen-containing functional groups by poorly crystalline mineral colloids served as the primary mechanism leading to their increased content levels. Changes in environmental factors significantly impacted the release and properties of soil colloids. Conditions such as low cationic valence, high ionic strength, and high pH promoted the release of soil colloids. Conclusions The short-term fertilization resulted in a reduction in the release of soil colloids and brought about significant alterations in their particle size composition and properties. The findings of this study provide valuable insights into understanding the impact of fertilization-induced colloid release on the environmental behavior of agricultural non-point source pollutants.

Abstract The auxin response factor (ARF) and auxin/indole-3-acetic acid (Aux/IAA) family of genes are central components of the auxin signaling pathway and play essential roles in plant growth and development. Their large-scale analysis and evolutionary trajectory of origin are currently not known. Here, we identified the corresponding ARF and Aux/IAA family members and performed a large-scale analysis by scanning 406 plant genomes. The results showed that the ARF and Aux/IAA gene families originated from charophytes. The ARF family sequences were more conserved than the Aux/IAA family sequences. Dispersed duplications were the common expansion mode of ARF and Aux/IAA families in bryophytes, ferns, and gymnosperms; however, whole-genome duplication was the common expansion mode of the ARF and Aux/IAA families in basal angiosperms, magnoliids, monocots, and dicots. Expression and regulatory network analyses revealed that the Arabidopsis thaliana ARF and Aux/IAA families responded to multiple hormone, biotic, and abiotic stresses. The APETALA2 and serum response factor-transcription factor gene families were commonly enriched in the upstream and downstream genes of the ARF and Aux/IAA gene families. Our study provides a comprehensive overview of the evolutionary trajectories, structural functions, expansion mechanisms, expression patterns, and regulatory networks of these two gene families.

With the rapid advancements in sequencing technology, an increasing number of genome sequences for medicinal plants have become available. In 2021, TCMPG was introduced as a comprehensive database dedicated to traditional Chinese medicine (TCM) plant genomes, capturing significant attention within the scientific community. To offer invaluable resources to researchers, this database received an upgrade to its latest version, named TCMPG 2.0, accessible at http://cbcb.cdutcm.edu.cn/TCMPG2/. TCMPG 2.0 surpasses its predecessor by adding 114 medicinal plants, 129 genomes, and 159 related herbs. Recognizing the critical role of ingredients in assessing the pharmacological effects of Chinese medicines, TCMPG 2.0 has also amassed data on 13,868 herbal ingredients. Additionally, four practical analytical tools, including Heatmap, Primer3, PlantiSMASH, and CRISPRCasFinder, have been integrated into the toolbox. These valuable enhancements significantly enhance the database's utility for researchers in the field.

The study was conducted to determine the nutrient digestibility and digestible (DE) and metabolizable energy (ME) of 15 cereal processing by-products and to develop prediction equations for DE and ME based on the chemical composition in growing pigs. A total of 36 crossbred pigs were chosen and divided into three blocks according to a three 12 × 3 Youdin square experimental design. Twelve pigs in each block were allocated into six diets including a basal diet and five test diets. The feeding experiment included three periods, and each dietary treatment contained six replicates. Each period lasted 12 days, including 7 d of dietary adaption and 5 d of total feces and urine collection. The results showed that the coefficients of variation for EE, NDF, ADF, CP, and GE among 15 cereal processing by-products were 86.78%, 49.82%, 51.14%, 14.95%, and 6.26%, respectively. There were significant differences in DE, ME, and ME/DE (DM basis) among 15 cereal processing by-products (p < 0.05), ranging from 11.55 MJ/kg to 17.64 MJ/kg, from 10.90 MJ/kg to 17.40 MJ/kg, and from 89.41% to 98.63%, respectively. The digestibility of GE, DM, NDF, ADF, and CP showed a significant difference among 15 cereal processing by-products (p < 0.05). The DE and ME contents showed a negative correlation to NDF and ADF (p < 0.05), and the prediction equations for DE and ME (as-fed basis) were DE (MJ/kg) = −0.4597 × ADF (%) + 0.5988 × GE (MJ/kg) + 6.0138 (R2 = 0.74; p < 0.05) and ME (MJ/kg) = 1.0440 × DE (MJ/kg) − 1.1235 (R2 = 0.98; p < 0.05). On a DM basis, the optimal prediction equations for DE and ME were DE (MJ/kg DM) = −0.1451 × NDF (%) + 0.3026 × CP (%) + 13.8595 (R2 = 0.72; p < 0.05) and ME (MJ/kg DM) = 1.1155 × DE (MJ/kg DM) + 0.0363 × ADF (%) − 2.3412 (R2 = 0.99; p < 0.05). In conclusion, there were large variations in the chemical composition, nutrients digestibility, and available energy among the 15 different samples of cereal processing by-products. It is reasonable to develop prediction equations for DE and ME based on the chemical composition of cereal by-products for growing pigs.

Fruit has an important role in human nutrition and health; therefore, the systematic study of fruit genomic data is essential. The Genomic Database of Fruits (TGDF, http://tgdf.bio2db.com/), established through whole-genome analyses of 44 fruit species, is a comprehensive, user-friendly fruit database. TGDF contains a wealth of functional genes, including 11,350 flowering genes, 3161 auxin signaling genes, 2164 anthocyanin synthesis genes, 1464 abscisic acid (ABA) synthesis genes, 10,931 ​cell division and expansion genes, 1786 starch synthesis genes, 294 fruit size genes, and 6311 sugar transporter genes. Additionally, TGDF contains 1,433,368 CRISPR guide sequences from various fruit genomes, along with information on homologous genes and duplication types for the 44 fruit species. TGDF contains 6,417,060 gene annotations sourced from TrEMBL, SwissProt, Nr, and Gene Ontology databases, along with tools such as Sequence Fetch, BLAST, Synteny, and JBrowse for bioinformatics analyses. Transcriptomic data were also collected and collated from fruits, including details on instruments, tissues, or growth stages. This comprehensive, user-friendly resource is the first collection of fruit genomic data. Users can easily download genomic sequences, gene annotations, and bioinformatics analysis results from TGDF, which will be updated continually. We anticipate that TGDF will become a primary resource for fruit comparative and functional genomic studies.

Summary: Stephania japonica is an early-diverging eudicotyledon plant with high levels of cepharanthine, proven to be effective in curing coronavirus infections. Here, we report a high-quality S. japonica genome. The genome size is 688.52 Mb, and 97.37% sequences anchor to 11 chromosomes. The genome comprises 67.46% repetitive sequences and 21,036 genes. It is closely related to two Ranunculaceae species, which diverged from their common ancestor 55.90–71.02 million years ago (Mya) with a whole-genome duplication 85.59–96.75 Mya. We further reconstruct ancestral karyotype of Ranunculales. Several cepharanthine biosynthesis genes are identified and verified by western blot. Two genes (Sja03G0243 and Sja03G0241) exhibit catalytic activity as shown by liquid chromatography-mass spectrometry. Then, cepharanthine biosynthesis genes, transcription factors, and CYP450 family genes are used to construct a comprehensive network. Finally, we construct an early-diverging eudicotyledonous genome resources (EEGR) database. As the first genome of the Menispermaceae family to be released, this study provides rich resources for genomic studies.

The objective of this study was to evaluate the nutritional values of three new corn varieties (high-iron corn, cadmium-resistant corn, low-phytate phosphorus corn) cultivated with molecular marker-assisted selection breeding technique fed to growing pigs and broilers. Exp. 1 was conducted to compare the nutritional values of high-iron corn, high-chromium corn, low-phytate phosphorus corn and conventional corn fed to growing pigs based on a 15 × 2 Youden square design. Exp.2 was conducted to compare the nutritional values of high-iron corn, low-phytate phosphorus corn and conventional corn fed to broilers based on a completely randomized design. Parameters including nutrient digestibility, available energy and amino acids, and mineral deposition were measured. The results shows that the iron content in the high-iron corn and the cadmium content in the cadmium-resistant corn were 29.608 mg/kg and 0.0057 mg/kg, respectively, both were greater than those in the other three kinds of corns. When fed to growing pigs, the neutral detergent fiber digestibility of the high-iron corn group was lower than that of the conventional corn group (p

Terpenes are natural secondary metabolites with isoprene as the basic structural unit; they are widely found in nature and have potential applications as advanced fuels, pharmaceutical ingredients, and agricultural chemicals. However, traditional methods are inefficient for obtaining terpenes because of complex processes, low yields, and environmental unfriendliness. The unconventional oleaginous yeast Yarrowia lipolytica, with a clear genetic background and complete gene editing tools, has attracted increasing attention for terpenoid synthesis. Here, we review the synthetic biology tools for Y. lipolytica, including promoters, terminators, selection markers, and autonomously replicating sequences. The progress and emerging trends in the metabolic engineering of Y. lipolytica for terpenoid synthesis are further summarized. Finally, potential future research directions are envisioned.

With global climate change, the frequency and intensity of waterlogging events are increasing due to frequent and heavy precipitation. Little is known however about the response of plants to repeated waterlogging stress events. The aim is to clarify physiological regulation mechanisms of tomato plants under repeated waterlogging stress, and whether Trichoderma harzianum can alleviate waterlogging injury. We identified two genotypes of tomato, ‘MIX-002’ and ‘LA4440’, as waterlogging tolerant and sensitive genotypes, respectively, based on plant biomass accumulation. The two tomato genotypes were subjected to a waterlogging priming treatment for 2 days (excess water for 1 cm above substrate surface) followed by a recovery stage for 2 days, and then a second waterlogging stress for 5 days (excess water for 1 cm above substrate surface) followed by a second recovery stage for 3 days. Leaf physiological, plant growth parameters, and the expression of five key genes were investigated. We found that the two genotypes responded differently to waterlogging priming and stress in terms of photosynthesis, reactive oxygen species (ROS), and osmotic regulatory mechanisms. Waterlogging stress significantly increased H2O2 content of ‘MIX-002’, while that of ‘LA4440’ had no significant change. Under waterlogging stress, photosynthesis of the two genotypes treated with waterlogging priming returned to the control level. However, Trichoderma harzianum treatment during the second recovery stage did not show positive mitigative effects. The plants of ‘LA4440’ with priming showed lower peroxidase (POD) activity and proline content but higher H2O2 content than that without priming under waterlogging stress. Under waterlogging stress with priming as compared to without priming, SODCC2 was downregulated in two tomatoes, and AGR2 and X92888 were upregulated in ‘MIX-002’ but downregulated in ‘LA4440’. Overall, the two tomato genotypes exhibited distinct photosynthetic, ROS and osmotic regulatory mechanisms responding to the waterlogging stress. Waterlogging priming can induce stress memory by adjusting stomatal conductance, sustaining ROS homeostasis, regulating osmotic regulatory substances and key gene expressions mediated by H2O2, and thus alleviate the damage on tomato photosynthesis when waterlogging reoccurred.

Abstract Carbon loading in airway cells has shown to worsen function of antimicrobial peptides, permitting increased survival of pathogens in the respiratory tract; however, data on the impacts of carbon particles on childhood acute respiratory infection (ARI) is limited. We assembled daily health data on outpatient visits for ARI (bronchitis, pneumonia, and total upper respiratory infection [TURI]) in children aged 0–14 years between 2015 and 2019 in Beijing, China. Anthropogenic carbons, including black carbon (BC) and its emission sources, and wood smoke particles (delta carbon, ultra‐violet absorbing particulate matter, and brown carbon) were continuously monitored. Using a time‐stratified case‐crossover approach, conditional logistic regression was performed to derive risk estimates for each outcome. A total of 856,899 children were included, and a wide range of daily carbon particle concentrations was observed, with large variations for BC (0.36–20.44) and delta carbon (0.48–57.66 μg/m3). Exposure to these particles were independently associated with ARI, with nearly linear exposure‐response relationships. Interquartile range increases in concentrations of BC and delta carbon over prior 0–8 days, we observed elevation of the odd ratio of bronchitis by 1.201 (95% confidence interval, 1.180, 1.221) and 1.048 (95% CI, 1.039, 1.057), respectively. Stronger association was observed for BC from traffic sources, which increased the odd ratio of bronchitis by 1.298 (95% CI, 1.273, 1.324). Carbon particles were also associated with elevated risks of pneumonia and TURI, and subgroup analyses indicated greater risks among children older than 6 years. Our findings suggested that anthropogenic carbons in metropolitan areas may pose a significant threat to clinical manifestations of respiratory infections in vulnerable populations.

Evidence of the precise biological pathway responsible for acute cardiovascular events triggered by particulate matter (PM) exposure from anthropogenic emissions is sparse. We investigated the associations of biomarkers relevant to the pathophysiology of atherothrombosis (ceramide metabolism, pro-inflammatory response, and blood coagulation) with primary and secondary components in particulate matter with aerodynamic diameters less than 2.5 μm (PM2.5). A total of 152 healthy participants were followed with four repeated clinical visits between September 2019 and January 2020 in Beijing. Exposure to ambient inorganic aerosols (sulfate, nitrate, ammonium, and chloride), as well as organic aerosols (OA) in PM2.5, was measured by a real-time aerosol chemical speciation monitor, and sources of OA were performed by positive matrix factorization. We found significant increases of 101.9–397.9% in ceramide indicators associated with interquartile-range increases in inorganic aerosols and OA prior to 72 h of exposure. Higher levels of organic and inorganic aerosols in PM2.5 were associated with increases of 3.1–6.0% in normal T cells regulated upon activation and expressed and secreted relevant to the pro-inflammatory response; increases of 276.9–541.5% were observed in D-dimers relevant to coagulation. Detrimental effects were further observed following OA exposure from fossil fuel combustion. Mediation analyses indicated that ceramide metabolism could mediate the associations of PM2.5 components with pro-inflammatory responses. Our findings expand upon the current understanding of potential pathophysiological pathways of cardiovascular events posed by ambient particulates and highlight the importance of reducing primary and secondary PM from anthropogenic combustions.

Materials with Weyl cones and band gaps coexisting are desired due to their combination of Weyl cones and the tunable and controllable nature of semiconductor. Here, the authors find Weyl-related band structures and semiconducting gap of tellurium.

Cucurbitaceae is one of the most important plant families distributed worldwide. Transcription factors (TFs) regulate plant growth at the transcription level. Here, we performed a systematic analysis of 42 641 TFs from 63 families in 14 Cucurbitaceae and 10 non-cucurbit species. Whole-genome duplication (WGD) was the dominant event type in almost all Cucurbitaceae plants. The TF families were divided into 1 210 orthogroups (OGs), of which, 112 were unique to Cucurbitaceae. Although the loss of several gene families was detected in Cucurbitaceae, the gene families expanded in five species that experienced a WGD event comparing with grape. Our findings revealed that the recent WGD events that had occurred in Cucurbitaceae played important roles in the expansion of most TF families. The functional enrichment analysis of the genes that significantly expanded or contracted uncovered five gene families, AUX/IAA, NAC, NBS, HB, and NF-YB. Finally, we conducted a comprehensive analysis of the TCP gene family and identified 16 tendril-related (TEN) genes in 11 Cucurbitaceae species. Interestingly, the characteristic sequence changed from CNNFYFP to CNNFYLP in the TEN gene (Bhi06M000087) of Benincasa hispida. Furthermore, we identified a new characteristic sequence, YNN, which could be used for TEN gene exploitation in Cucurbitaceae. In conclusion, this study will serve as a reference for studying the relationship between gene family evolution and genome duplication. Moreover, it will provide rich genetic resources for functional Cucurbitaceae studies in the future.

Abstract Background Brassinosteroids (BRs) are a group of essential steroid hormones involved in diverse developmental and physiological processes in plants. The Brassinazole-resistant 1 (BZR1) transcription factors are key components of BR signaling and integrate a wide range of internal and environmental signals to coordinate plant development, growth, and resistance to abiotic and biotic stresses. Although the BZR1 family has been fully studied in Arabidopsis, celery BZR1 family genes remain largely unknown. Results Nine BZR1 genes were identified in the celery genome, and categorized into four classes based on phylogenetic and gene structure analyses. All the BZR1 proteins shared a typical bHLH (basic helix-loop-helix) domain that is highly conserved across the whole family in Arabidopsis, grape, lettuce, ginseng, and three Apiaceae species. Both duplications and losses of the BZR1 gene family were detected during the shaping of the celery genome. Whole-genome duplication (WGD) or segmental duplication contributed 55.56% of the BZR1 genes expansion, and the γ as well as celery-ω polyploidization events made a considerable contribution to the production of the BZR1 paralogs in celery. Four AgBZR1 members (AgBZR1.1, AgBZR1.3, AgBZR1.5, and AgBZR1.9), which were localized both in the nucleus and cytoplasm, exhibit transcription activation activity in yeast. AgBZR1.5 overexpression transgenic plants in Arabidopsis showed curled leaves with bent, long petioles and constitutive BR-responsive phenotypes. Furthermore, the AgBZR1 genes possessed divergent expression patterns with some overlaps in roots, petioles, and leaves, suggesting an extensive involvement of AgBZR1s in the developmental processes in celery with both functional redundancy and divergence. Conclusions Our results not only demonstrated that AgBZR1 played a conserved role in BR signaling but also suggested that AgBZR1 might be extensively involved in plant developmental processes in celery. The findings lay the foundation for further study on the molecular mechanism of the AgBZR1s in regulating the agronomic traits and environmental adaptation of celery, and provide insights for future BR-related genetic breeding of celery and other Apiaceae crops.

Abstract Background When researchers perform gene family analysis, they often analyze the structural characteristics of the gene, such as the distribution of introns and exons. At the same time, characteristic structural analysis of amino acid sequence is also essential, for example, motif and domain features. Researchers often integrate these analyses into one image to dig out more information, but the tools responsible for this integration are lacking. Results Here, we developed a tool (CFVisual) for drawing gene structure and protein architecture. CFVisual can draw the phylogenetic tree, gene structure, and protein architecture in one picture, and has rich interactive capabilities, which can meet the work needs of researchers. Furthermore, it also supports arbitrary stitching of the above analysis images. It has become a useful helper in gene family analysis. The CFVisual package was implemented in Python and is freely available from https://github.com/ChenHuilong1223/CFVisual/ . Conclusion CFVisual has been used by some researchers and cited by some articles. In the future, CFVisual will continue to serve as a good helper for researchers in the study of gene structure and protein architecture.

Objective: The exact biological mechanism whereby exposure to ambient ozone (O3) may contribute to clinical onset of cardiovascular events remains unclear. In this study, we aim to examine the impacts of O3 exposure on cardiac arrhythmias and potential pathways involved through autonomic dysfunction and myocardial injury. Methods: Seventy-three non-smoking healthy adults were followed with 4 repeated measurements of 24-hour ambulatory arrhythmias, heart rate variability, ST-segment deviation, and blood pressure (BP) in Beijing, China, 2014‒2016. Generalized additive mixed models coupled with distributed lag nonlinear models were constructed to evaluate the associations and potential interlinks between O3 exposure and outcome measurements. Results: During the study period, 24-hour average concentrations of ambient O3 were 47.4 µg/m3 (ranging from 1.0 to 165.9 µg/m3). Increased risks of premature ventricular contraction and ventricular tachycardia were associated with interquartile range increases in O3 exposure during the last 5 days before each participant's clinic visit, with relative risks of 2.14 (95% confidence interval [CI]: 1.95 to 2.32) and 5.47 (95% CI: 3.51 to 7.43), respectively. Mediation analyses further showed that sympathetic activation, parasympathetic inhibition, and elevated BP levels, as well as heightened risks of ST-segment depression could mediate up to 47.74% of the risks of arrhythmias attributable to O3 exposure. Conclusion: Our results suggest that short-term exposure to ambient O3 could prompt the genesis of arrhythmias partially through worsening autonomic function and myocardial burden.

Objective: Evidence on potential cardiovascular benefits of personal-level intervention among the elderly exposed to high levels of particulate matter (PM) remains limited. We aimed to assess improvements in surrogate markers of cardiovascular injury in vulnerable populations at risks by using indoor air filtration units. Methods: We conducted a randomized crossover trial for 2 separate 2-week air filtration interventions in 20 households of patients with stable chronic obstructive pulmonary disease and their partners in the winter of 2013, with concurrent measurements of indoor PM. The changes in biomarkers indicative of cardiac injury, atherosclerosis progression and systemic inflammation following intervention were evaluated using linear mixed-effect models. Results: In the analysis, average levels of indoor PM with aerodynamic diameters

Many topological semimetals are excellent thermoelectric materials, but previous studies were limited to steady-state properties. Here, the authors observe a transient thermoelectric response in Cd3As2 by detecting the resulting THz emission, with an enhanced response when a small magnetic field is applied.

BackgroundThe increasing threats of air pollution and extreme weather have been widely recognized in recent years in China, but their individual and joint effects on cardio-cerebrovascular mortality are unclear.ObjectiveThis study aims to investigate the individual effects of and potential interactions between oxidant pollutants and ambient temperature on cardio-cerebrovascular mortality risks.MethodsWe collected daily data on death counts of cardio-cerebrovascular diseases, concentrations of ambient air pollutants, and meteorological parameters in Guangzhou, Chinabetween 1 January 2006 and 31 December 2016. A generalized additive model with a Poisson distribution was conducted to assess the associations of oxidant pollutants and ambient temperature with cardio-cerebrovascular mortality risks. Bivariate response surface models and stratified analyses were further adopted to qualitatively and quantitatively examine the potential interactions between oxidant pollutants and ambient temperature on cardio-cerebrovascular mortality risks.ResultsDuring the study period, the daily averages were 60.3 μg·m−3 for ozone (O3), 50.9 μg·m−3 for combined atmospheric oxidant capacity (Ox), 32.5 μg·m−3 for nitrogen dioxide (NO2), and 22.3℃ for ambient temperature. The average daily death counts of coronary and stroke diseases were 20 and 15, respectively. Per 10 μg·m−3 increment in O3, Ox, and NO2 were associated with increased coronary mortality risks (excess risk, ER) of 1.26% (95%CI: 0.79%-1.74%), 1.61% (95%CI: 0.99%-2.23%), and 1.33% (95%CI: 0.59%-2.07%), and with increased stroke mortality risks of 1.56% (95%CI: 1.04%-2.09%), 2.30% (95%CI: 1.60%-3.01%), and 2.93% (95%CI: 2.07%-3.79%) over cumulative lags of 2-5 days, respectively. The exposure-response relationships between ambient temperature and coronary and stroke mortality risks exhibited an inverse "J" shape, with the minimum mortality at temperatures of 25.7℃ for coronary disease and 27.3℃ for stroke. Our results further showed potentially synergic effects of higher temperatures and higher levels of O3 and Ox exposures on coronary mortality risks, and the relative ER due to interactions was 0.103 (95%CI: 0.028-0.178) for O3 and 0.079 (95%CI: 0.004-0.154) for Ox. We didn't find evidence of an interaction between oxidant pollutants and low temperature.ConclusionShort-term exposures to oxidant pollutants are associated with increased cardio-cerebrovascular mortality risks, and the interactive effects of high temperature and oxidant pollutants are synergistic in relation to cardio-cerebrovascular mortality risks.

Apiaceae is a major family from Apiales and includes many important vegetable and medicinal crops. Heat shock transcription factors (Hsf) play important roles in heat tolerance during plant development. Here, we conducted systematic analyses of the Hsf gene family in three Apiaceae species, including 17 Apium graveolens (celery), 32 Coriandrum sativum (coriander), and 14 Daucus carota (carrot). A total of 73 Hsf genes were identified in three representative species, including Arabidopsis thaliana, Vitis vinifera, and Lactuca sativa. Whole-genome duplication played important roles in the Hsf gene family's expansion within Apiaceae. Interestingly, we found that coriander had more Hsf genes than celery and carrot due to greater expansion and fewer losses. Twenty-seven branches of the phylogenetic tree underwent considerable positive selection in these Apiaceae species. We also explored the expression patterns of Hsf genes in three plant organs. Collectively, this study will serve as a rich gene resource for exploring the molecular mechanisms of heat tolerance. Additionally, this is the first study to report on the Hsf gene family in Apiaceae; thus, our research will provide guidance for future comparative and functional genomic studies on the Hsf gene family and others in Apiaceae.

“The Expert Group on Tumor Ablation Therapy of Chinese Medical Doctor Association, The Tumor Ablation Committee of Chinese College of Interventionalists, The Society of Tumor Ablation Therapy of Chinese Anti-Cancer Association and The Ablation Expert Committee of the Chinese Society of Clinical Oncology” have organized multidisciplinary experts to formulate the consensus for thermal ablation of pulmonary subsolid nodules or ground-glass nodule (GGN). The expert consensus reviews current literatures and provides clinical practices for thermal ablation of GGN. The main contents include: (1) clinical evaluation of GGN, (2) procedures, indications, contraindications, outcomes evaluation and related complications of thermal ablation for GGN and (3) future development directions.

Spacer grid with mixing vane had been widely used in nuclear reactor core. One of the main feather of spacer grid with mixing vane was that strong swirl flow was formed after the spacer grid. The swirl flow not only changed the bubble generation in the near wall field, but also affected the bubble behaviors in the center region of the subchannel. The interaction between bubble and the swirl flow was one of the basic phenomena for the two phase flow modeling in fuel assembly. To obatin better understanding on the bubble behaviors in swirl flow, full three dimension numerical simulations were conducted in the present paper. The swirl flow was assumed in the cylindral calculation domain. The bubble interface was captured by Volume Of Fluid (VOF) method. The properties of saturated water and steam at different pressure were applied in the simulation. The bubble trajectory, motion, shape and force were obtained based on the bubble parameters captured by VOF. The simulation cases in the present study included single bubble with different size, at different angular velocity conditions and at different pressure conditions. The results indicated that bubble migrated to the center in swirl flow with spiral motion type. The lateral migration was mainly related to shear stress magnitude and bubble size. The bubble moved toward the center with high velocity when the swirl magnitude was high. The largest bubble had the highest lateral migration velocity in the present study range. The effect of pressure was small when bubble size was the same. The prelimenery simulation result would be beneficial for better understanding complex two phase flow phenomena in fuel assembly with spacer grid.

Simple sequence repeats (SSRs) are popular and important molecular markers that exist widely in plants. Here, we conducted a comprehensive identification and comparative analysis of SSRs in 14 tree species. A total of 16, 298 SSRs were identified from 429, 449 genes, and primers were successfully designed for 99.44% of the identified SSRs. Our analysis indicated that tri-nucleotide SSRs were the most abundant, with an average of ~834 per species. Functional enrichment analysis by combining SSR-containing genes in all species, revealed 50 significantly enriched terms, with most belonging to transcription factor families associated with plant development and abiotic stresses such as Myeloblastosis_DNA-bind_4 (Myb_DNA-bind_4), APETALA2 (AP2), and Fantastic Four meristem regulator (FAF). Further functional enrichment analysis showed that 48 terms related to abiotic stress regulation and floral development were significantly enriched in ten species, whereas no significantly enriched terms were found in four species. Interestingly, the largest number of enriched terms was detected in Citrus sinensis (L.) Osbeck, accounting for 54.17% of all significantly enriched functional terms. Finally, we analyzed AP2 and trihelix gene families (Myb_DNA-bind_4) due to their significant enrichment in SSR-containing genes. The results indicated that whole-genome duplication (WGD) and whole genome triplication (WGT) might have played major roles in the expansion of the AP2 gene family but only slightly affected the expansion of the trihelix gene family during evolution. In conclusion, the identification and comprehensive characterization of SSR markers will greatly facilitate future comparative genomics and functional genomics studies.

Polyploids generated by the replication of a single genome (autopolyploid) or synthesis of two or more distinct genomes (allopolyploid) usually show significant advantages over their diploid progenitors in biological characteristics, including growth and development, nutrient accumulation, and plant resistance. Whereas, the impacts of genomic replication on transcription regulation and chromatin structure in pak choi have not been explored fully. In this study, we observed the transcriptional and genomic structural alterations between diploid B. rapa (AA) and artificial autotetraploid B. rapa (AAAA) using RNA-seq and Hi-C. RNA-seq revealed 1,786 differentially expressed genes (DEGs) between the diploids and autotetraploids, including 717 down-regulated and 1,069 up-regulated genes in autotetraploids. Of all the 1,786 DEGs, 23 DEGs (10 down-regulated DEGs in autotetraploids) were involved in Compartment A-B shifts, while 28 DEGs (20 up-regulated DEGs in autotetraploids) participated in Compartment B-A shifts. Moreover, there were 15 DEGs in activated topologically associating domains (TADs) (9 up-regulated DEGs in diploids) and 80 DEGs in repressed TADs (49 down-regulated DEGs in diploids). Subsequently, eight DEGs with genomic structural variants were selected as potential candidate genes, including four DEGs involved in photosynthesis (BraA01003143, BraA09002798, BraA04002224, and BraA08000594), three DEGs related to chloroplast (BraA05002974, BraA05001662, and BraA04001148), and one DEG associated with disease resistance (BraA09004451), which all showed high expression in autotetraploids. Overall, our results demonstrated that integrative RNA-seq and Hi-C analysis can identify related genes to phenotypic traits and also provided new insights into the molecular mechanism of the growth advantage of polyploids.

Varieties of various crops with high resilience are urgently needed to feed the increased population in climate change conditions. Human activities and climate change have led to frequent and strong weather fluctuation, which cause various abiotic stresses to crops. The understanding of crops’ responses to abiotic stresses in different aspects including genes, RNAs, proteins, metabolites, and phenotypes can facilitate crop breeding. Using multi-omics methods, mainly genomics, transcriptomics, proteomics, metabolomics, and phenomics, to study crops’ responses to abiotic stresses will generate a better, deeper, and more comprehensive understanding. More importantly, multi-omics can provide multiple layers of information on biological data to understand plant biology, which will open windows for new opportunities to improve crop resilience and tolerance. However, the opportunities and challenges coexist. Interpretation of the multidimensional data from multi-omics and translation of the data into biological meaningful context remained a challenge. More reasonable experimental designs starting from sowing seed, cultivating the plant, and collecting and extracting samples were necessary for a multi-omics study as the first step. The normalization, transformation, and scaling of single-omics data should consider the integration of multi-omics. This review reports the current study of crops at abiotic stresses in particular heat stress using omics, which will help to accelerate crop improvement to better tolerate and adapt to climate change.

Lycophytes and seed plants constitute the typical vascular plants. Lycophytes have been thought to have no paleo-polyploidization although the event is known to be critical for the fast expansion of seed plants. Here, genomic analyses including the homologous gene dot plot analysis detected multiple paleo-polyploidization events, with one occurring approximately 13–15 million years ago (MYA) and another about 125–142 MYA, during the evolution of the genome of Selaginella moellendorffii, a model lycophyte. In addition, comparative analysis of reconstructed ancestral genomes of lycophytes and angiosperms suggested that lycophytes were affected by more paleo-polyploidization events than seed plants. Results from the present genomic analyses indicate that paleo-polyploidization has contributed to the successful establishment of both lineages—lycophytes and seed plants—of vascular plants.

Abstract Background Brassica is a very important genus of Brassicaceae, including many important oils, vegetables, forage crops, and ornamental horticultural plants. TLP family genes play important regulatory roles in the growth and development of plants. Therefore, this study used a bioinformatics approach to conduct the systematic comparative genomics analysis of TLP gene family in B. napus and other three important Brassicaceae crops. Results Here, we identified a total of 29 TLP genes from B. napus genome, and they distributed on 16 chromosomes of B. napus. The evolutionary relationship showed that these genes could be divided into six groups from Group A to F. We found that the gene corresponding to Arabidopsis thaliana AT1G43640 was completely lost in B. rapa, B. oleracea and B. napus after whole genome triplication. The gene corresponding to AT1G25280 was retained in all the three species we analysed, belonging to 1:3:6 ratios. Our analyses suggested that there was a selective loss of some genes that might be redundant after genome duplication. This study proposed that the TLP genes in B. napus did not directly expansion compared with its diploid parents B. rapa, and B. oleracea. Instead, an indirect expansion of TLP gene family occurred in its two diploid parents. In addition, the study further utilized RNA-seq to detect the expression pattern of TLP genes between different tissues and two subgenomes. Conclusions This study systematically conducted the comparative analyses of TLP gene family in B. napus, discussed the loss and expansion of genes after genome duplication. It provided rich gene resources for exploring the molecular mechanism of TLP gene family. Meanwhile, it provided guidance and reference for the research of other gene families in B. napus.

Background: Emerging studies have investigated potential cardiovascular and respiratory health impacts from the use of personal-level intervention equipment against air pollution exposure. The objective of this systematic review is to assess the efficacy of personal-level air pollution intervention on mitigating adverse health effects from air pollution exposure by using portable air cleaner or wearing respirator. Methods: In this systematic review, we searched PubMed and Web of Science for published literatures up to May 31, 2020, focusing on personal-level air pollution intervention studies. Among these studies, we investigated the impacts on cardio-respiratory responses to the use of these interventions. The intervention of review interest was the use of personal-level equipment against air pollution, including using portable air cleaner indoors or wearing respirator outdoors. The outcome of review interest was impacts on cardio-respiratory health endpoints following interventions, including level changes in blood pressure, heart rate variability (HRV), lung function, and biomarkers of inflammation and oxidative stress. Weighted mean differences or percent changes were pooled in meta-analyses for these health endpoints. The heterogeneity across studies was assessed using the Cochran's Q-statistic test, and the individual study quality was assessed using the Cochrane risk of bias tool version 2 (RoB 2). We further applied the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) method to evaluate the certainty of evidence. Results: From systematic literature search and screening, we identified 29 related eligible intervention studies, including 21 studies on indoor portable air cleaner use and 8 studies on respirator use. For portable air cleaner intervention, we observed suggestive evidence of beneficial changes on cardio-respiratory health endpoints. Collectively in these studies, we found significantly beneficial changes of 2.01% decreases (95% CI: 0.50%, 3.52%) in systolic blood pressure, as well as non-significantly beneficial changes of 3.04% increases (95% CI: -2.65%, 8.74%) in reactive hyperemia index and 0.24% increases (95% CI: −0.82%, 1.31%) in forced expiratory volume in 1 s. We also observed non-significant reductions in levels of inflammation and oxidative stress biomarkers, including C-reactive protein, interleukin-6, fibrinogen, fractional exhaled nitric oxide and malondialdehyde. For respirator intervention, we observed some beneficial changes on cardiovascular health endpoints, such as significant increases in HRV parameters [SDNN (2.20%, 95% CI: 0.54%, 3.86%)], as well as non-significant decreases in blood pressure [SBP (0.63 mmHg, 95% CI: −0.39, 1.66)]; however, no sufficient data were available for meta-analyses on lung function and biomarkers. RoB 2 assessments suggested that most intervention studies were with a moderate to high overall risk of bias. The certainty of evidence for intervention outcome pairs was graded very low for either portable air cleaner or respirator intervention. The common reasons to downgrade study evidence included loss to follow-up, lack of blinding, lack of washout period, small sample size, and high heterogeneity across studies. Conclusions: The uses of indoor portable air cleaner and respirator could contribute to some beneficial changes on cardiovascular health, but with much limited evidence on respiratory health. Low certainty of the overall study evidence shed light on future research for larger sample size trials with more rigorous study design.

Cabbage (Brassica oleracea L. var. capitata L.) is an important cruciferous vegetable, which is rich in dietary fiber, vitamins, beta-carotene and even good for cancer prevention. So, it is widely planted around the world. However, in the production of cabbage, the property of head-splitting not only reduces the appearance quality and commercial value of cabbage but also easily infects the area with head-splitting by germs, resulting in yield reduction, even no yield, and economic losses. For the head-splitting of cabbage, this paper introduced the causes, evaluation methods and indexes, and anatomic properties and reviewed some QTLs that have been obtained. But the cabbage molecular research of head-splitting is still in its infancy compared with other vegetable plants. Head-splitting is greatly affected by water. Although the cultivation and management methods can reduce the head-splitting rate, genetic improvement of head-splitting is still the most economic and efficient way in the long run. Therefore, the changes in the substances regulating cell water potential, the development of cell wall, ductility and toughness, calcium ion-regulated pectinase and cellulase activities, expansin genes, and even the ETH and ABA pathways related to maturation and abscission can provide ideas and directions for future gene mining and mechanism analysis for head-splitting tolerance, and thus accelerate the molecular breeding process.

As an essential post-transcriptional modification, N7-methylguanosine (m7G) regulates nearly every step of the life cycle of mRNA. Accurate identification of the m7G site in the transcriptome will provide insights into its biological functions and mechanisms. Although the m7G-methylated RNA immunoprecipitation sequencing (MeRIP-seq) method has been proposed in this regard, it is still cost-ineffective for detecting the m7G site. Therefore, it is urgent to develop new methods to identify the m7G site. In this work, we developed the first computational predictor called iRNA-m7G to identify m7G sites in the human transcriptome. The feature fusion strategy was used to integrate both sequence- and structure-based features. In the jackknife test, iRNA-m7G obtained an accuracy of 89.88%. The superiority of iRNA-m7G for identifying m7G sites was also demonstrated by comparing with other methods. We hope that iRNA-m7G can become a useful tool to identify m7G sites. A user-friendly web server for iRNA-m7G is freely accessible at http://lin-group.cn/server/iRNA-m7G/. Keywords: N7-methylguanosine, nucleotide chemical property, RNA secondary structure, pseudo nucleotide composition, feature fusion

RNA N2-methylguanosine (m2G) is one kind of posttranscriptional modification and plays crucial roles in the control and stabilization of tRNA. However, our knowledge about the biological functions of m2G is still limited. The key step of revealing its new function is to recognize the m2G sites in the transcriptome. Since there is no effective method for detecting m2G sites, it is desirable to develop new methods to identify m2G sites. In this study, a computational predictor called iRNA-m2G was proposed to identify m2G sites in eukaryotic transcriptomes. In iRNA-m2G, the RNA sequences were encoded by using nucleotide chemical property and accumulated nucleotide frequency. iRNA-m2G was not only validated by the rigorous jackknife test on the benchmark dataset but also examined by performing cross-species validations. In addition, iRNA-m2G was also tested on an independent dataset. It was found that the accuracies obtained by iRNA-m2G were all quite promising in these tests, indicating that the proposed method could become a powerful tool for identifying m2G sites. Finally, a user-friendly web server for iRNA-m2G is freely accessible at http://lin-group.cn/server/iRNA-m2G.php. Keywords: N2-methylguanosine, nucleotide chemical property, accumulated nucleotide frequency, web server

Numerous epidemiologic studies on adverse health effects of air pollution have been well documented; however, assessment on health benefits of air quality improvement from air pollution control measures has been limited in developing countries.We assessed the mortality benefits associated with air pollution improvement over 11 years in Guangzhou, China (2006–2016).A time series analysis with Generalized additive Poisson models was used to estimate mortality effects of ozone (O3) and nitrogen dioxide (NO2), adjusting for time trend, day of week, public holiday, temperature and relative humidity. We further estimated the changes in mortality burden of O3 and NO2, including attributable fraction (AF, in %) and attributable mortality (AM, in number of death) during study period. We lastly estimated mortality effects during the 2010 Asian Games (November 12 to December 18, 2010) compared to a baseline period consisting of 4-week before and 4-week after the game.During the study period, average annual concentrations of NO2 decreased from 42.3 μg/m3 in 2006 to 33.8 μg/m3 in 2016; while O3 levels remained stable over time. We observed significant increases in mortality of O3 and NO2, with approximately linear exposure-response relationships. In specific, each increase of 10 μg/m3 in O3 and NO2 at 2 prior days was associated with increases of 0.60% (95% confidence interval (CI): 0.47, 0.74) and 1.89% (95%CI: 1.49, 2.29) in total mortality, respectively. We further estimated that AF on total mortality attributed to NO2 decreased from 1.38% (95%CI: 1.09, 1.68) in 2006–2010 to 0.43% (95%CI: 0.34, 0.52) in 2011–2016, corresponding to AM on total mortality of 2496 deaths (95%CI: 1964, 3033) to 1073 deaths (95%CI: 846, 1301). During the 2010 Asian Games, we observed decrease in total mortality of 9.3% (95%CI: −15.0, −3.2) in comparison with that observed in the baseline period. Similar mortality benefits in cardiovascular diseases were also observed.Our results showed reduced mortality burden from air pollution improvement in Guangzhou in recent years, which provide strong rationale for continuing to reduce air pollution through comprehensive and rigorous air quality management in the area. Keywords: Air quality improvement, Mortality burden, Health benefit, Asian games, Time-series analysis

Abstract Background After polyploidization, a genome may experience large-scale genome-repatterning, featuring wide-spread DNA rearrangement and loss, and often chromosome number reduction. Grasses share a common tetraploidization, after which the originally doubled chromosome numbers reduced to different chromosome numbers among them. A telomere-centric reduction model was proposed previously to explain chromosome number reduction. With Brachpodium as an intermediate linking different major lineages of grasses and a model plant of the Pooideae plants, we wonder whether it mediated the evolution from ancestral grass karyotype to Triticeae karyotype. Results By inferring the homology among Triticeae, rice, and Brachpodium chromosomes, we reconstructed the evolutionary trajectories of the Triticeae chromosomes. By performing comparative genomics analysis with rice as a reference, we reconstructed the evolutionary trajectories of Pooideae plants, including Ae. Tauschii (2n = 14, DD), barley (2n = 14), Triticum turgidum (2n = 4x = 28, AABB), and Brachypodium (2n = 10). Their extant Pooidea and Brachypodium chromosomes were independently produced after sequential nested chromosome fusions in the last tens of millions of years, respectively, after their split from rice. More frequently than would be expected by chance, in Brachypodium, the ‘invading’ and ‘invaded’ chromosomes are homoeologs, originating from duplication of a common ancestral chromosome, that is, with more extensive DNA-level correspondence to one another than random chromosomes, nested chromosome fusion events between homoeologs account for three of seven cases in Brachypodium (P-value≈0.00078). However, this phenomenon was not observed during the formation of other Pooideae chromosomes. Conclusions Notably, we found that the Brachypodium chromosomes formed through exclusively distinctive trajectories from those of Pooideae plants, and were well explained by the telomere-centric model. Our work will contribute to understanding the structural and functional innovation of chromosomes in different Pooideae lineages and beyond.

In the high-temperature and high-pressure irradiation environments, the multi-field coupling processes of hydrogen diffusion, hydride precipitation and mechanical deformation in Zircaloy cladding tubes occur. To simulate this hydrogen-induced complex behavior, a multi-field coupling method is developed, with the irradiation hardening effects and hydride-precipitation-induced expansion and hardening effects involved in the mechanical constitutive relation. The out-pile tests for a cracked cladding tube after irradiation are simulated, and the numerical results of the multi-fields at different temperatures are obtained and analyzed. The results indicate that: (1) the hydrostatic stress gradient is the fundamental factor to activate the hydrogen-induced multi-field coupling behavior excluding the temperature gradient; (2) in the local crack-tip region, hydrides will precipitate faster at the considered higher temperatures, which can be fundamentally attributed to the sensitivity of TSSP and hydrogen diffusion coefficient to temperature. The mechanism is partly explained for the enlarged velocity values of delayed hydride cracking (DHC) at high temperatures before crack arrest. This work lays a foundation for the future research on DHC. Keywords: Numerical simulation, Multi-field coupling, DHC, Irradiation effects, Hydride-precipitation-induced effects

In this paper, an adaptive nonsingular terminal sliding mode control (ANTSMC) is investigated for attitude tracking of spacecraft with actuator faults. First, a nonsingular fast terminal sliding mode surface is designed to avoid the singularity. Finite-time attitude control is developed using the nonsingular terminal sliding mode technique, which can make the attitude and angular velocity tracking errors converge to zero in finite time in the presence of uncertainties and external disturbances. Second, the total uncertainty is deduced to be bounded. The adaptive laws are incorporated to develop the ANTSMC, removing the restriction on the upper bound of the lumped uncertainty. The finite-time convergence of the closed-loop system with ANTSMC is proved using the Lyapunov stability theory. Finally, the simulation results are presented to demonstrate the performance of the proposed controllers.

Instantaneous volatility of logarithmic return in the lognormal fractional SABR model is driven by the exponentiation of a correlated fractional Brownian motion. Due to the mixed nature of driving Brownian and fractional Brownian motions, probability density for such a model is less studied in the literature. We show in this paper a bridge representation for the joint density of the lognormal fractional SABR model in a Fourier space. Evaluating the bridge representation along a properly chosen deterministic path yields a small time asymptotic expansion to the leading order for the probability density of the fractional SABR model. A direct generalization of the representation of joint density often leads to a heuristic derivation of the large deviations principle for joint density in a small time. Approximation of implied volatility is readily obtained by applying the Laplace asymptotic formula to the call or put prices and comparing coefficients.

Existing hyperchaotic systems suffer from a small parameter range and small key space. Therefore, we propose herein a novel wide-range discrete hyperchaotic map(3D-SCC) based on the mathematical model of the Sine map. Dynamic numerical analysis shows that this map has a wide-range of parameters, high sensitive, high sensitivity of sequences and good ergodicity, which proves that the system is well suited to the field of communication encryption. Moreover, this paper proposes an image encryption algorithm based on a dynamic cycle shift scramble algorithm and image-sensitive function. First, the image feature is extracted by the image-sensitive function to input into the chaos map. Then, the plaintext image is decomposed by an integer wavelet, and the low-frequency part is scrambled by a dynamic cyclic shifting algorithm. The shuffled low-frequency part and high-frequency parts are reconstructed by wavelet, and the chaotic matrix image is bitwise XOR with it to obtain the final ciphertext. The experimental results show that the average NPCR is 99.6024%, the average UACI is 33.4630%, and the average local Shannon entropy is 7.9029, indicating that the statistical properties of the ciphertext are closer to the ideal value. The anti-attack test shows that the algorithm can effectively resist cutting attacks and noise attacks. Therefore, the algorithm has great application value in the field of image encryption.

The family Apiaceae includes many important vegetables and medicinal plants. Auxin response factors (ARFs) play critical roles in regulating plant growth and development. Here, we performed a comprehensive analysis of the ARF gene family in three Apiaceae species, celery, coriander, and carrot, and compared the results with the ARF gene family of lettuce, Arabidopsis, and grape. We identified 156 ARF genes in all six species and 89 genes in the three Apiaceae species, including 28, 34, and 27 in celery, coriander, and carrot, respectively. The paralogous gene number in coriander was far greater than that in carrot and celery. Our analysis revealed that ARF genes of the three Apiaceae species in 34 branches of the phylogenetic tree underwent significant positive selection. Additionally, our findings indicated that whole-genome duplication played an important role in ARF gene family expansion. Coriander contained a greater number of ARF genes than celery and carrot because of more gene duplications and less gene losses. We also analyzed the expression of ARF genes in three tissues by RNA-seq and verified the results by quantitative real-time PCR. Furthermore, we found that several paralogous genes exhibited divergent expression patterns. Overall, this study provides a valuable resource for exploring how ARF family genes regulate plant growth and development in other plants. Since this is the first report of the ARF gene family in Apiaceae, our results will serve as a guide for comparative and functional analyses of ARF and other gene families in Apiaceae.

As one of the major contributors to the early failures of steel bridge deck pavements, the bonding between steel and asphalt overlay has long been a troublesome issue. In this paper, a novel composite bonding structure was introduced consisting of epoxy resin micaceous iron oxide (EMIO) primer, solvent-free epoxy resin waterproof layer, and ethylene-vinyl acetate (EVA) hot melt pellets. A series of strength tests were performed to study its mechanical properties, including pull-off strength tests, dumbbell tensile tests, lap shear tests, direct tension tests, and 45°-inclined shear tests. The results suggested that the bonding structure exhibited fair bonding strength, tensile strength, and shear strength. Anisotropic behaviour was also observed at high temperatures. For epoxy resin waterproof layer, the loss of bonding strength, tensile strength, and shear strength at 60°C was 70%, 35%, and 39%, respectively. Subsequent pavement performance-oriented tests included five-point bending tests and accelerated wheel tracking tests. The impacts of bonding on fatigue resistance and rutting propagation were studied. It was found that the proposed bonding structure could provide a durable and well-bonded interface and was thus beneficial to prolong the fatigue lives of asphalt overlay. The choice of bonding materials was found irrelevant to the ultimate rutting depth of pavements. But the bonding combination of epoxy resin waterproof and EVA pellets could delay the early-stage rutting propagation.

Vapor pressure deficit (VPD) is the driver of water movement in plants. However, little is known about how anatomical adaptations determine the acclimation of plant water dynamics to elevated VPD, especially at the whole plant level. Here, we examined the responses of transpiration, stomatal conductance (gs), hydraulic partitioning, and anatomical traits in two tomato cultivars (Jinpeng and Zhongza) to long-term high (2.2–2.6 kPa) and low (1.1–1.5 kPa) VPD. Compared to plants growing under low VPD, no variation in gs was found for Jinpeng under high VPD conditions; however, high VPD induced an increase in whole plant hydraulic conductance (Kplant), which was responsible for the maintenance of high transpiration. In contrast, transpiration was not influenced by high VPD in Zhongza, which was primarily attributed to a coordinated decline in gs and Kplant. The changes in gs were closely related to stomatal density and size. Furthermore, high VPD altered hydraulic partitioning among the leaf, stem, and root for both cultivars via adjustments in anatomy. The increase in lumen area of vessels in veins and large roots in Jinpeng under high VPD conditions improved water transport efficiency in the leaf and root, thus resulting in a high Kplant. However, the decreased Kplant for Zhongza under high VPD was the result of a decline of water transport efficiency in the leaf that was caused by a reduction in vein density. Overall, we concluded that the tradeoff in anatomical acclimations among plant tissues results in different water relations in plants under high VPD conditions.

The refined characterization of groundwater pollution is an important prerequisite for efficient and effective remediation. A high-resolution survey of a contaminated site in a chemical pesticide factory was carried out using non-invasive geophysical sensing technology. Modern electrical resistivity tomography (ERT) technology can rapidly identify and characterize the groundwater pollution plumes of organochlorine pesticides, which was demonstrated in this study by the significantly abnormal resistivity sensing in stratums and aquifers under the raw material tanks, production, and loading areas. The results were found to be highly consistent with the ERT sensing results achieved via incorporating borehole sampling and hydrochemical analysis. With high abnormal resistivity, the range of contamination within the profile was characterized on the meter level. We also unexpectedly found new pollution and explained its source. This study confirmed that the modern refined ERT method has a high feasibility and accuracy in characterizing the spatial distribution of organochlorine pesticide plumes in groundwater.

Atmospheric vapor pressure deficit (VPD) is the driving force that regulates the rate of water transport within plants. Under High VPD (HVPD), plants always reduce their photosynthesis rate and close their stomata. Experiments were performed under greenhouse conditions with cucumber and tomato plants to identify the regulatory effect of VPD on plant water capacity. Treatments included two levels of soil water (100% and 60% field capacity [FC]) combined with two levels of VPD (LVPD and HVPD). Results indicated that with 60%FC, the plant heights of tomato and cucumber were enhanced under LVPD compared with those under HVPD. With 60%FC, relative leaf water contents under LVPD increased by 11% compared with those under HVPD. Furthermore, LVPD significantly improved the photosynthetic capacity of the two crops and changed their stress responses. Our results indicated that LVPD at different soil moisture levels reduced irrigation demand under greenhouse conditions. This approach can be applied in water management in greenhouse vegetable production in China and other regions of the world with temperate continental climates.

Abstract Background Trihelix transcription factors (TTF) play important roles in plant growth and response to adversity stress. Until now, genome-wide identification and analysis of this gene family in foxtail millet has not been available. Here, we identified TTF genes in the foxtail millet and its grass relatives, and characterized their functional domains. Results As to sequence divergence, TTF genes were previously divided into five subfamilies, I-V. We found that Trihelix family members in foxtail millet and other grasses mostly preserved their ancestral chromosomal locations during millions of years’ evolution. Six amino acid sites of the SIP1 subfamily possibly were likely subjected to significant positive selection. Highest expression level was observed in the spica, with the SIP1 subfamily having highest expression level. As to the origination and expansion of the gene family, notably we showed that a subgroup of subfamily IV was the oldest, and therefore was separated to define a new subfamily O. Overtime, starting from the subfamily O, certain genes evolved to form subfamilies III and I, and later from subfamily I to develop subfamilies II and V. The oldest gene, Si1g016284, has the most structural changes, and a high expression in different tissues. What’s more interesting is that it may have bridge the interaction with different proteins. Conclusions By performing phylogenetic analysis using non-plant species, notably we showed that a subgroup of subfamily IV was the oldest, and therefore was separated to define a new subfamily O. Starting from the subfamily O, certain genes evolved to form other subfamilies. Our work will contribute to understanding the structural and functional innovation of Trihelix transcription factor, and the evolutionary trajectory.

Firstly,the 5G standard and industrial progress were summarized,and the 5G core network architecture,key technologies and interworking architecture were combed.On this basis,the idea of phased evolution planning and deployment suggestions for the 5G core network were proposed to provide reference for the introduction of the 5G core network in the present network.

Standalone (SA) deployment and non-standalone (NSA) deployment are the key issues considered in the early stage of 5G network introduction.Firstly,the deployment architecture options in 3GPP standard were introduced and the key deployment architectures were analyzed and compared.Then two evolution routes of 5G network deployment were put forward.At last,the deployment mode and evolution strategy of 5G core network were given,which provided references for the deployment mode and evolution path selection of 5G network.

Abstract Background The BES1 gene family, an important class of plant-specific transcription factors, play key roles in the BR signal pathway in plants, regulating various development processes. Until now, there has been no comprehensive analysis of the BES1 gene family in Brassica napus, and a cross-genome exploration of their origin, copy number changes, and functional innovation in plants was also not available. Results We identified 28 BES1 genes in B. napus from its two subgenomes (AA and CC). We found that 71.43% of them were duplicated in the tetraploidization, and their gene expression showed a prominent subgenome bias in the roots. Additionally, we identified 104 BES1 genes in another 18 representative angiosperms and performed a comparative analysis with B. napus, including evolutionary trajectory, gene duplication, positive selection, and expression pattern. Exploiting the available genome datasets, we performed a large-scale analysis across plants and algae suggested that the BES1 gene family could have originated from group F, expanding to form other groups (A to E) by duplicating or alternatively deleting some domains. We detected an additional domain containing M4 to M8 in exclusively groups F1 and F2. We found evidence that whole-genome duplication (WGD) contributed the most to the expansion of this gene family among examined dicots, while dispersed duplication contributed the most to expansion in certain monocots. Moreover, we inferred that positive selection might have occurred on major phylogenetic nodes during the evolution of plants. Conclusions Grossly, a cross-genome comparative analysis of the BES1 genes in B. napus and other species sheds light on understanding its copy number expansion, natural selection, and functional innovation.

The responses of tomato seedlings to different vapour pressure deficit (VPD) under low soil moisture were studied. Plants were grown in greenhouses with low and high VPD, under both well-watered and water stress conditions. Low VPD was effectively maintained below 1.5 kPa with a micro-fog system. Under well-watered conditions, low VPD resulted in reduced transpiration, but this did not affect plant water status or growth. Water stress induced leaf dehydration and inhibition of growth, but the adverse effects were significantly alleviated by a decrease in VPD. Under water stress, no difference in transpiration was observed between plants with or without the VPD regulation, but the whole-plant hydraulic conductance was higher under low VPD. Low VPD increased stomatal conductance in drought-stressed plants because it promoted stomatal development and increased stomatal aperture. Thus, stomatal limitation to photosynthesis was reduced by low VPD under water stress. The reduction in plant growth induced by water stress was moderated by low VPD, partially due to higher photosynthetic rate. These results suggest that decreasing VPD improves plant water status, which ultimately enhances photosynthesis and growth under water stress.