15 results on '"Xianliang Lai"'
Search Results
2. Mechanism of ischemic brain injury repair by endothelial progenitor cell‑derived exosomes
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Rui, Huang, Tianxiang, Cheng, and Xianliang, Lai
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Vascular Endothelial Growth Factor A ,Cancer Research ,Glycogen Synthase Kinase 3 beta ,Infarction, Middle Cerebral Artery ,Exosomes ,Biochemistry ,Rats ,Rats, Sprague-Dawley ,Oncology ,Brain Injuries ,Reperfusion Injury ,Genetics ,Animals ,Molecular Medicine ,RNA, Messenger ,Molecular Biology ,Endothelial Progenitor Cells - Abstract
Ischemic stroke is a refractory disease that seriously endangers human health and life. The main treatment aim of stroke is to alleviate brain injury. The present study aimed to investigate the effects and mechanisms of endothelial progenitor cell (EPC)‑derived exosomes in repairing ischemic brain injury. Sprague‑Dawley rat models of cerebral ischemia‑reperfusion (IR) injury were established by middle cerebral artery occlusion. The IR model rats were then treated with PBS, EPC or exosomes; untreated and Sham rats were used as control. EPCs were obtained from tibias and femurs, and exosomes were isolated from the EPCs and characterized. To measure brain injury, 2,3,5‑triphenyltetrazolium chloride staining was used to measure the infarct area, neurological deficit was scored, hematoxylin and eosin staining was used to examine pathological changes and TUNEL staining was used to quantify apoptosis. Immunofluorescence staining and reverse transcription‑quantitative PCR were used to determine CD31 and VEGF protein and mRNA expressions, respectively, and western blot analysis was used out to measure the protein expression levels of Wnt3a, GSK‑3β and phosphorylated (p)‑GSK‑3β. Compared with rats in the Control and Sham groups, in IR model rats the nerve fibers were slightly necrotic and swollen and the number of nerve cells was reduced. Following EPC treatment, the brain tissue exhibited mild liquefaction and degeneration in the small focus area with mild edema in the stroma. The numbers of nerve cells decreased, and the distribution of nerve cells was not very uniform; proliferation of glial cells was observed. Following treatment with exosomes, the distribution of nerve cells was more uniform with less degeneration and necrosis; the proliferation of glial cells was remarkable. Compared with the Control group, the infarct size, neurological defect score, percentage of apoptotic cells, expression of CD31, VEGF, Wnt3a, and p‑GSK‑3β were significantly higher in the IR model (P0.05). After EPC and exosome treatments, the infarct size, neurological defect score, percentage of apoptotic cells, expression of Wnt3a, and p‑GSK‑3β were significantly reduced (P0.05), whereas the mRNA and protein expression levels of CD31 and VEGF were significantly increased (P0.05). Results from the present study demonstrated that EPC‑derived exosomes may alleviate ischemic injury by inhibiting apoptosis and promoting angiogenesis. These findings suggested that exosomes may have a protective role for nerve cells and may be a potentially effective option for treating stroke. However, human clinical studies are needed to validate these findings from animals.
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- 2022
3. Regulatory mechanism of MiR-21 in formation and rupture of intracranial aneurysm through JNK signaling pathway-mediated inflammatory response
- Author
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Zhihua, Chen, Shuxin, Song, Jianming, Zhu, and Xianliang, Lai
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Original Article - Abstract
Objective: To investigate the regulatory mechanism of micro ribonucleic acid (miR)-21 in the formation and rupture of intracranial aneurysm through the c-Jun N-terminal kinase (JNK) signaling pathway-mediated inflammatory response. Methods: In the present study, the mice with miR-21 expression deficiency and over-expression in our laboratory were enrolled as the experimental group, while wild-type healthy mice were used as the control group. The mouse model of intracranial aneurysm was established by bilateral carotid artery ligation. The differences in the levels of key genes in the JNK signaling pathway (JNK1 and JNK2) were detected by fluorescence quantitative polymerase chain reaction (qPCR) and western blotting. At the same time, the changes in transcription and translation levels of inflammatory factors, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), in both groups were measured. After the mice were executed by an overdose of anesthesia, the morphology of the aneurysm in different objects was observed by Verhoeff-Van Gieson (EVG) staining and the expressions of TNF-α, JNK1, and JNK2 were determined by immunohistochemistry. Results: Compared with healthy mice, levels of JNK1 and JNK2 in mice with miR-21 deficiency were significantly decreased (P < 0.05) with a significant reduction of inflammatory factors IL-6 and TNF-α (P < 0.05). Compared with healthy mice, levels of JNK1 and JNK2 in mice with miR-21 over-expression were significantly increased (P < 0.05) with significant growing levels of inflammatory factors IL-6 and TNF-α (P < 0.05). The results of EVG staining revealed that the intracranial aneurysm was smaller in mice with miR-21 deficiency [(0.3 ± 0.12) cm] and larger in mice with miR-21 over-expression [(0.8 ± 0.25) cm] and there was a significant difference (P < 0.05). Moreover, the results of immunohistochemistry showed that the expression of TNF-α in intracranial aneurysm was obviously lower in mice with miR-21 deficiency than that in mice with miR-21 over-expression. Conclusion: MiR-21 can promote the production of inflammation-related factors through the JNK signaling pathway, leading to the formation and rupture of an intracranial aneurysm.
- Published
- 2019
4. Apc gene suppresses intracranial aneurysm formation and rupture through inhibiting the NF-κB signaling pathway mediated inflammatory response
- Author
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Xianliang Lai, Xingen Zhu, Zhihua Chen, and Zhifeng Deng
- Subjects
0301 basic medicine ,Tumor suppressor gene ,Adenomatous polyposis coli ,Biophysics ,Inflammation ,Biochemistry ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,NF-κB signaling pathway ,Molecular Biology ,Research Articles ,biology ,business.industry ,Inflammatory response ,Interleukin ,Cell Biology ,Intracranial aneurysm ,Apc gene ,IκBα ,030104 developmental biology ,biology.protein ,Cancer research ,Tumor necrosis factor alpha ,Signal transduction ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Research Article - Abstract
Background: Intracranial aneurysm (IA) is a critical acquired cerebrovascular disease that may cause subarachnoid hemorrhage, and nuclear factor-κB (NF-κB)-mediated inflammation is involved in the pathogenesis of IA. Adenomatous polyposis coli (Apc) gene is a tumor suppressor gene associated with both familial and sporadic cancer. Herein, the purpose of our study is to validate effect of Apc gene on IA formation and rupture by regulating the NF-κB signaling pathway mediated inflammatory response. Methods: We collected IA specimens (from incarceration of IA) and normal cerebral arteries (from surgery of traumatic brain injury) to examine expression of Apc and the NF-κB signaling pathway related factors (NF-κB p65 and IκBα). ELISA was used to determine levels of monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β (IL-1β), and IL-6. IA model was established in rats, and Apc-siRNA was treated to verify effect of Apc on IA formation and rupture. Next, regulation of Apc on the NF-κB signaling pathway was investigated. Results: Reduced expression of Apc and IκBα, and increased expression of NF-κB p65 were found in IA tissues. MCP-1, TNF-α, IL-1β, and IL-6 exhibited higher levels in unruptured and ruptured IA, which suggested facilitated inflammatory responses. In addition, the IA rats injected with Apc-siRNA showed further enhanced activation of NF-κB signaling pathway, and up-regulated levels of MCP-1, TNF-α, IL-1β, IL-6, MMP-2, and MMP-9 as well as extent of p65 phosphorylation in IA. Conclusion: Above all, Apc has the potential role to attenuate IA formation and rupture by inhibiting inflammatory response through repressing the activation of the NF-κB signaling pathway.
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- 2019
5. Nanostructured lipid carriers, solid lipid nanoparticles, and polymeric nanoparticles: which kind of drug delivery system is better for glioblastoma chemotherapy?
- Author
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Jianming Zhu, Zhihua Chen, Liangqiao Zhang, Xianliang Lai, Jie Qu, Xingen Zhu, Ziyun Gao, and Guohua Mao
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Drug ,Materials science ,Polymers ,Chemistry, Pharmaceutical ,media_common.quotation_subject ,Mice, Nude ,Pharmaceutical Science ,Gliomatosis cerebri ,Antineoplastic Agents ,02 engineering and technology ,Pharmacology ,030226 pharmacology & pharmacy ,Mice ,03 medical and health sciences ,Drug Delivery Systems ,0302 clinical medicine ,Cell Line, Tumor ,Glioma ,Solid lipid nanoparticle ,Temozolomide ,medicine ,Zeta potential ,Animals ,Humans ,Particle Size ,media_common ,Drug Carriers ,Mice, Inbred BALB C ,General Medicine ,021001 nanoscience & nanotechnology ,medicine.disease ,Lipids ,Nanostructures ,Dacarbazine ,Drug delivery ,Nanoparticles ,Nanocarriers ,Glioblastoma ,0210 nano-technology ,medicine.drug - Abstract
Glioblastoma is a malignant brain tumor originating in the central nervous system. Successfully therapy of this disease required the efficient delivery of therapeutic agents to the tumor cells and tissues. Delivery of anticancer drugs using novel nanocarriers is promising in glioma treatment.Polymeric nanoparticles (PNPs), solid lipid nanoparticles (SLNs), and nanostructured lipid carriers (NLCs) were constructed for the delivery of temozolomide (TMZ). The anti-tumor effects of the three kinds of nanocarriers were compared to provide the optimum choice for gliomatosis cerebri treatment.TMZ-loaded PNPs (T-PNPs), SLNs (T-SLNs), and NLCs (T-NLCs) were formulated. Their particle size, zeta potential, drug encapsulation efficiency (EE), and drug loading (DL) capacity were evaluated. Anti-tumor efficacies of the three kinds of nanocarriers were evaluated on U87 malignant glioma cells (U87 MG cells) and mice-bearing malignant glioma model.T-NLCs displayed the best anti-tumor activity than other formulations in vivo and in vitro. The most significantly glioma inhibition was observed on NLCs formulations than PNPs and SLNs.This work demonstrates that NLCs can deliver TMZ into U87MG cells more efficiently, with higher inhibition efficacy than PNPs and SLNs. T-NLCs could be an excellent drug delivery system for glioblastoma chemotherapy.
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- 2016
6. Lactoferrin- and RGD-comodified, temozolomide and vincristine-coloaded nanostructured lipid carriers for gliomatosis cerebri combination therapy
- Author
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Ziyun Gao, Guohua Mao, Xianliang Lai, Jicai Zhang, Jianming Zhu, Xingen Zhu, and Xiang Xiao
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0301 basic medicine ,nanostructured lipid carriers ,Pharmaceutical Science ,gliomatosis cerebri ,02 engineering and technology ,temozolomide ,combination therapy ,Drug Delivery Systems ,International Journal of Nanomedicine ,Drug Discovery ,Antineoplastic Combined Chemotherapy Protocols ,Tissue Distribution ,Cytotoxicity ,Original Research ,Liposome ,Drug Carriers ,Mice, Inbred BALB C ,Chemistry ,Brain Neoplasms ,General Medicine ,021001 nanoscience & nanotechnology ,Lipids ,Neoplasms, Neuroepithelial ,lactoferrin ,Dacarbazine ,Drug delivery ,0210 nano-technology ,Drug carrier ,Oligopeptides ,medicine.drug ,Combination therapy ,Biophysics ,Gliomatosis cerebri ,Bioengineering ,vincristine ,Biomaterials ,03 medical and health sciences ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Temozolomide ,Organic Chemistry ,medicine.disease ,Xenograft Model Antitumor Assays ,Nanostructures ,Drug Liberation ,030104 developmental biology ,Cancer research ,arginine–glycine–aspartic acid peptide - Abstract
Jicai Zhang, Xiang Xiao, Jianming Zhu, Ziyun Gao, Xianliang Lai, Xingen Zhu, Guohua Mao Department of Neurosurgery, Second Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China Purpose: Glioblastoma multiforme (GBM) is the most common malignant brain tumor originating in the central nervous system in adults. Based on nanotechnology such as liposomes, polymeric nanoparticles, and lipid nanoparticles, recent research efforts have been aimed to target drugs to the brain. Methods: In this study, lactoferrin- and arginine–glycine–aspartic acid (RGD) dual-ligand-comodified, temozolomide and vincristine-coloaded nanostructured lipid carriers (L/R-T/V-NLCs) were introduced for GBM combination therapy. The physicochemical properties of L/R-T/V-NLCs such as particle size, zeta potential, and encapsulated efficiency are measured. The drug release profile, cellular uptake, cytotoxicity, tissue distribution, and antitumor activity of L/R-T/V-NLCs are further investigated in vitro and in vivo. Results: L/R-T/V-NLCs were stable with nanosize and high drug encapsulation efficiency. L/R-T/V-NLCs exhibited sustained-release behavior, high cellular uptake, high cytotoxicity and synergy effects, increased drug accumulation in the tumor tissue, and obvious tumor inhibition efficiency with low systemic toxicity. Conclusion: L/R-T/V-NLCs could be a promising drug delivery system for glioblastoma chemotherapy. Keywords: gliomatosis cerebri, combination therapy, nanostructured lipid carriers, lactoferrin, arginine–glycine–aspartic acid peptide, vincristine, temozolomide
- Published
- 2018
7. Nanostructured lipid carriers based temozolomide and gene co-encapsulated nanomedicine for gliomatosis cerebri combination therapy
- Author
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Shuxin Song, Xingen Zhu, Xianliang Lai, Zhihua Chen, and Jianming Zhu
- Subjects
Materials science ,Chemistry, Pharmaceutical ,Genetic enhancement ,Pharmaceutical Science ,Antineoplastic Agents ,02 engineering and technology ,Pharmacology ,Transfection ,Mice ,03 medical and health sciences ,Drug Delivery Systems ,0302 clinical medicine ,In vivo ,Cell Line, Tumor ,Glioma ,Temozolomide ,medicine ,Animals ,Humans ,Drug Carriers ,DNA ,General Medicine ,021001 nanoscience & nanotechnology ,medicine.disease ,Lipids ,Neoplasms, Neuroepithelial ,Nanostructures ,Dacarbazine ,Nanomedicine ,030220 oncology & carcinogenesis ,Cancer cell ,Nanocarriers ,0210 nano-technology ,medicine.drug - Abstract
Co-delivery of gene and anticancer drug into the same cancer cells or tissues by multifunctional nanocarriers may provide a new paradigm in cancer treatment. In this study, nanostructured lipid carriers (NLCs) were constructed as multifunctional nanomedicine for co-delivery of enhanced green fluorescence protein plasmid (DNA) and temozolomide (TMZ).TMZ- and DNA-loaded NLCs (TMZ/DNA-NLCs) were prepared. Their particle size, zeta potential, gene-loading capacity (GL) and drug encapsulation efficiency (EE) were evaluated. In vitro cytotoxicity study TMZ/DNA-NLCs was tested in U87 malignant glioma cells (U87 MG cells). In vivo gene transfection and anti-tumor efficacy of the carriers were evaluated on mice bearing malignant glioma model.The optimum TMZ/DNA-NLCs formulations with the particle size of 179 nm and with a +23 mV surface charge; got 91% of GL and 83% of EE. The growth of U87 MG cells in vitro was obviously inhibited. TMZ/DNA-NLCs also displayed the highest gene transfection efficiency and the best antitumor activity than other formulations in vivo.The results demonstrated that TMZ/DNA-NLCs were efficient in selective delivery to malignant glioma cells. Also TMZ/DNA-NLCs transfer both drug and gene to the gliomatosis cerebri, enhance the antitumor capacity and gene transfection efficacy. Thus, TMZ/DNA-NLCs could prove to be a superior co-delivery nanomedicine to achieve therapeutic efficacy and this report could be a new promising strategy for treatment in malignant gliomatosis cerebri.
- Published
- 2015
8. A Novel Anterior Transpedicular Screw Artificial Vertebral Body System for Lower Cervical Spine Fixation: A Finite Element Study
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Changfu Wu, Zhang Jinyuan, Chun Chen, Weidong Wu, Jun Ouyang, Jihong Fan, Jinpei Ning, Zhenyu Bi, Xianliang Lai, and Peidong Sun
- Subjects
musculoskeletal diseases ,Materials science ,Bone Screws ,Finite Element Analysis ,Biomedical Engineering ,Finite element study ,Facet joint ,03 medical and health sciences ,Fixation (surgical) ,0302 clinical medicine ,Physiology (medical) ,medicine ,von Mises yield criterion ,Range of Motion, Articular ,Mechanical Phenomena ,Orthodontics ,030222 orthopedics ,musculoskeletal system ,Cervical spine ,Vertebral body ,medicine.anatomical_structure ,Cervical Vertebrae ,Stress, Mechanical ,Cadaveric spasm ,Range of motion ,030217 neurology & neurosurgery - Abstract
A finite element model was used to compare the biomechanical properties of a novel anterior transpedicular screw artificial vertebral body system (AVBS) with a conventional anterior screw plate system (ASPS) for fixation in the lower cervical spine. A model of the intact cervical spine (C3–C7) was established. AVBS or ASPS constructs were implanted between C4 and C6. The models were loaded in three-dimensional (3D) motion. The Von Mises stress distribution in the internal fixators was evaluated, as well as the range of motion (ROM) and facet joint force. The models were generated and analyzed by mimics, geomagic studio, and ansys software. The intact model of the lower cervical spine consisted of 286,382 elements. The model was validated against previously reported cadaveric experimental data. In the ASPS model, stress was concentrated at the connection between the screw and plate and the connection between the titanium mesh and adjacent vertebral body. In the AVBS model, stress was evenly distributed. Compared to the intact cervical spine model, the ROM of the whole specimen after fixation with both constructs is decreased by approximately 3 deg. ROM of adjacent segments is increased by approximately 5 deg. Facet joint force of the ASPS and AVBS models was higher than those of the intact cervical spine model, especially in extension and lateral bending. AVBS fixation represents a novel reconstruction approach for the lower cervical spine. AVBS provides better stability and lower risk for internal fixator failure compared with traditional ASPS fixation.
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- 2017
9. HP1α is highly expressed in glioma cells and facilitates cell proliferation and survival
- Author
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Xingen Zhu, Zhifeng Deng, Xianliang Lai, Wei Tu, and Hua Guo
- Subjects
0301 basic medicine ,Cancer Research ,Cell Survival ,Chromosomal Proteins, Non-Histone ,Biophysics ,Gene Expression ,Apoptosis ,Biochemistry ,Epigenesis, Genetic ,Histones ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Cell Line, Tumor ,Puma ,Glioma ,Genetics ,medicine ,Humans ,Epigenetics ,Promoter Regions, Genetic ,Molecular Biology ,Cell Proliferation ,SUV39H1 ,biology ,Brain Neoplasms ,Cell growth ,Brain ,Methyltransferases ,Cell Biology ,General Medicine ,biology.organism_classification ,medicine.disease ,Up-Regulation ,Repressor Proteins ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Histone ,Oncology ,Chromobox Protein Homolog 5 ,030220 oncology & carcinogenesis ,Mutation ,Cancer research ,biology.protein - Abstract
Epigenetic alteration plays critical roles in gliomagenesis by regulating gene expression through modifications of Histones and DNA. Trimethylation of H3K9, an essential repressed transcription mark, and one of its methyltransferase, SUV39H1, are implicated in glioma pathogenesis and progression. We find that the protein level of HP1α, a reader of H3K9me3 is elevated in GOS3 and 1321N1 glioma cell lines. H3K9me3 and SUV39H1 level are also upregulated. Depletion of HP1α and SUV39H1 weakens GOS3 and 1321N1 cell proliferation capacity and results in apoptosis of cells. Furthermore, we find that HP1α and H3K9me3 are enriched in the FAS and PUMA promoters, which suggests that upregulated HP1α and H3K9me3 prevent apoptosis by suppressing apoptotic activators. These data indicates that up-regulated HP1α, SUV39H1, and H3K9me3 in glioma cells are functionally associated with glioma pathogenesis and progression, and may serve as novel biomarkers for future diagnostic and therapeutic targeting of brain tumors.
- Published
- 2018
10. Mesenchymal stem cells regulate the proliferation and differentiation of neural stem cells through Notch signaling
- Author
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Zhifeng Deng, Wei Tu, Yuanlei Lou, An Xie, Xianliang Lai, Fei Guo, and Yang Wang
- Subjects
Neurogenesis ,Notch signaling pathway ,Bone Marrow Cells ,Mice ,Neurosphere ,Paracrine Communication ,Basic Helix-Loop-Helix Transcription Factors ,Animals ,HES1 ,Receptor, Notch1 ,reproductive and urinary physiology ,Cell Proliferation ,Cerebral Cortex ,Homeodomain Proteins ,Neurons ,Glial fibrillary acidic protein ,biology ,Stem Cells ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Cell Biology ,General Medicine ,Neural stem cell ,Coculture Techniques ,Cell biology ,Ki-67 Antigen ,nervous system ,biology.protein ,NIH 3T3 Cells ,Transcription Factor HES-1 ,biological phenomena, cell phenomena, and immunity ,Stem cell ,Signal Transduction - Abstract
The effects of mesenchymal stem cells (MSCs) on proliferation and cell fate determination of neural stem cells (NSCs) have been investigated. NSCs were co-cultured with MSCs or NIH3T3 cells using an in vitro transwell system. After 4 days, immunofluorescence staining showed that the number of cells positive for the cell proliferation antigen, ki-67, in neurospheres in MSCs was greater than in NIH3T3 cells. In some experiments, the top-layers of MSCs and NIH3T3 cells were removed to induce NSCs differentiation. Seven days after initiating differentiation, the levels of the neuronal marker, NSE, were higher in NSCs in MSCs co-culture group, and those of glial fibrillary acidic protein (GFAP) were lower, compared with NIH3T3 cells co-culture group. These were confirmed by immunofluorescence. The role of the Notch signaling pathway analyzed with the specific inhibitor, DAPT, and by examining the expression of Notch-related genes using RT-PCR showed that after co-culturing with MSCs for 24h, NSCs expressed much higher levels of ki-67, Notch1, and Hes1 than did NSCs co-cultured with NIH3T3 cells. Treatment with DAPT decreased ki-67, Notch1 and Hes1 expression in NCSs, and increased Mash1 expression. The data indicate that the interactions between MSCs and NSCs promote NSCs proliferation and are involved in specifying neuronal fate, mediated in part by Notch signaling.
- Published
- 2009
11. Differentiation of human bone marrow stromal cells into neural-like cells induced by sodium ferulate in vitro
- Author
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Yang, Wang, Zhifeng, Deng, Xianliang, Lai, and Wei, Tu
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Neurons ,Formazans ,Coumaric Acids ,Cell Survival ,Phosphopyruvate Hydratase ,Glial Fibrillary Acidic Protein ,Humans ,Tetrazolium Salts ,Bone Marrow Cells ,Cell Differentiation ,Stromal Cells ,Biomarkers - Abstract
Human marrow stromal cells (hMSCs) are multipotential stem cells, capable of differentiating into bone, cartilage, fat and muscle. Several recent reports demonstrated that hMSCs have been also differentiated into neural cells. However, only a few reported inducers are applicable for clinical use. This work is to explore the effects of sodium ferulate (SF) on differentiation of hMSCs into neural cells in vitro. We found that hMSCs could be induced to the cells with typical neural morphology when cultured with SF. The cells express neural proteins, such as nestin, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP). About 30% of the hMSC-derived cells expressed nestin when cultured with SF for 3 h, but no expression was detected after 24 h. The percentages of positive cells for NSE or GFAP were about 67% and 39% separately at 6 h, and reached the plateau phage after treatment with SF for 3 days. The data suggest that SF can induce hMSCs to differentiate into neural-like cells in vitro.
- Published
- 2005
12. HP1α is highly expressed in glioma cells and facilitates cell proliferation and survival.
- Author
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Xianliang Lai, Zhifeng Deng, Hua Guo, Xingen Zhu, and Wei Tu
- Subjects
- *
GLIOMAS , *BRAIN tumors , *GLIOMA treatment , *ISOCITRATE dehydrogenase , *DIAGNOSIS - Abstract
Epigenetic alteration plays critical roles in gliomagenesis by regulating gene expression through modifications of Histones and DNA. Trimethylation of H3K9, an essential repressed transcription mark, and one of its methyltransferase, SUV39H1, are implicated in glioma pathogenesis and progression. We find that the protein level of HP1α, a reader of H3K9me3 is elevated in GOS3 and 1321N1 glioma cell lines. H3K9me3 and SUV39H1 level are also upregulated. Depletion of HP1α and SUV39H1 weakens GOS3 and 1321N1 cell proliferation capacity and results in apoptosis of cells. Furthermore, we find that HP1α and H3K9me3 are enriched in the FAS and PUMA promoters, which suggests that upregulated HP1α and H3K9me3 prevent apoptosis by suppressing apoptotic activators. These data indicates that up-regulated HP1α, SUV39H1, and H3K9me3 in glioma cells are functionally associated with glioma pathogenesis and progression, and may serve as novel biomarkers for future diagnostic and therapeutic targeting of brain tumors. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
13. A Novel Anterior Transpedicular Screw Artificial Vertebral Body System for Lower Cervical Spine Fixation: A Finite Element Study.
- Author
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Weidong Wu, Chun Chen, Jinpei Ning, Peidong Sun, Jinyuan Zhang, Changfu Wu, Zhenyu Bi, Jihong Fan, Xianliang Lai, and Jun Ouyang
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- 2017
- Full Text
- View/download PDF
14. Sodium ferulate induces bone marrow mesenchymal stem cells to differentiate into neural cells by NF-κB signal pathway
- Author
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Yang Wang, Yuanlei Lou, Xianliang Lai, and Zhifeng Deng
- Subjects
chemistry.chemical_compound ,chemistry ,Immunology ,NF-κB ,Cell Biology ,Biology ,Molecular Biology ,Sodium ferulate ,Signal pathway ,Bone marrow mesenchymal stem cells ,Cell biology - Abstract
Sodium ferulate induces bone marrow mesenchymal stem cells to differentiate into neural cells by NF-κB signal pathway
- Published
- 2008
15. Sodium ferulate induces bone marrow mesenchymal stem cells to differentiate into neural cells by NF-κB signal pathway.
- Author
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Yuanlei Lou, Yang Wang, Xianliang Lai, and Zhifeng Deng
- Subjects
BONE marrow cells ,IMMUNOGLOBULINS ,IMMUNOFLUORESCENCE ,STAINS & staining (Microscopy) ,STEM cells - Abstract
Bone marrow stromal cells (MSCs) are nonhematopoietic stem cells in bone marrow, capable of self-renew and with pluripotency to differentiate into various cell types. A number of publications have proved that MSCs can be induced into progenitors of neural cells, neurons and glia by some inductors. In our previous study, we demonstrated that sodium ferulate (SF) could induce MSCs to differentiate into neural-like cells in vitro. However, the mechanism about the action of SF on inducing MSCs to undergo neurogenic differentiation is unclear. The present study was to explore whether the NF-βB signaling pathway contributes to the differentiation of rat MSCs (rMSCs ) into neurallike cells induced by SF. rMSCs were cultured in the medium with 1 mg/ml of SF. The results of morphologia observation showed rMSCs change obviously from flat, elongated cells to spherical and refractile cells with several branching extensions, exhibiting a typical neuronsal morphology after treated with SF for 6 h. The number of neurallike cells increased significantly with time and their processes formed extensive networks during the induction process. Immunofluorescence staining showed that rMSCs induced by SF gradually increased the expression of nestin, characteristic of neuronal precursor stem cells, as well as neuron-specific enolase (NSE), a marker of mature neurons, and glial fibrillary acidic protein (GFAP), the mature astrocyte marker. Immunofluorescence staining also showed that NF-βB/p65 protein was located in nuclear in a majority of rMSCs exposured to SF for 30 min, whereas NF-βB/p65 protein was located in plasm in a majority of rMSCs cultured without SF. Western blot analysis also showed the expression of NF-βB/p65 in the nuclear was at high level in rMSCs treated with SF. Our data indicate that NF-βB signaling pathway may contribute to the differentiation of rMSCs into neural-like cells induced by SF. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
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