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Nanostructured lipid carriers based temozolomide and gene co-encapsulated nanomedicine for gliomatosis cerebri combination therapy
- Source :
- Drug Delivery. 23:1369-1373
- Publication Year :
- 2015
- Publisher :
- Informa UK Limited, 2015.
-
Abstract
- Co-delivery of gene and anticancer drug into the same cancer cells or tissues by multifunctional nanocarriers may provide a new paradigm in cancer treatment. In this study, nanostructured lipid carriers (NLCs) were constructed as multifunctional nanomedicine for co-delivery of enhanced green fluorescence protein plasmid (DNA) and temozolomide (TMZ).TMZ- and DNA-loaded NLCs (TMZ/DNA-NLCs) were prepared. Their particle size, zeta potential, gene-loading capacity (GL) and drug encapsulation efficiency (EE) were evaluated. In vitro cytotoxicity study TMZ/DNA-NLCs was tested in U87 malignant glioma cells (U87 MG cells). In vivo gene transfection and anti-tumor efficacy of the carriers were evaluated on mice bearing malignant glioma model.The optimum TMZ/DNA-NLCs formulations with the particle size of 179 nm and with a +23 mV surface charge; got 91% of GL and 83% of EE. The growth of U87 MG cells in vitro was obviously inhibited. TMZ/DNA-NLCs also displayed the highest gene transfection efficiency and the best antitumor activity than other formulations in vivo.The results demonstrated that TMZ/DNA-NLCs were efficient in selective delivery to malignant glioma cells. Also TMZ/DNA-NLCs transfer both drug and gene to the gliomatosis cerebri, enhance the antitumor capacity and gene transfection efficacy. Thus, TMZ/DNA-NLCs could prove to be a superior co-delivery nanomedicine to achieve therapeutic efficacy and this report could be a new promising strategy for treatment in malignant gliomatosis cerebri.
- Subjects :
- Materials science
Chemistry, Pharmaceutical
Genetic enhancement
Pharmaceutical Science
Antineoplastic Agents
02 engineering and technology
Pharmacology
Transfection
Mice
03 medical and health sciences
Drug Delivery Systems
0302 clinical medicine
In vivo
Cell Line, Tumor
Glioma
Temozolomide
medicine
Animals
Humans
Drug Carriers
DNA
General Medicine
021001 nanoscience & nanotechnology
medicine.disease
Lipids
Neoplasms, Neuroepithelial
Nanostructures
Dacarbazine
Nanomedicine
030220 oncology & carcinogenesis
Cancer cell
Nanocarriers
0210 nano-technology
medicine.drug
Subjects
Details
- ISSN :
- 15210464 and 10717544
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Drug Delivery
- Accession number :
- edsair.doi.dedup.....c8ba5e8e55bbffe3e1dc94cc2c536fd7