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An experimental study of 14 keV O− ions transmission through a tapered glass capillary with the inlet diameters of 580 μm, an outlet diameter of 68 μm and a length of 45 mm, has been performed in this work. It indicates that the O− ions transmitting through the tapered capillary had a density enhancement of about 5.5 times with the tilt angle of 0°. It was found that more than 97% of the transmitted and focused ions remained their initial charge state in central area. It’s the first time that focusing effect was observed and was reported in experiment for negative ions. The angular distribution of transmitted ions was narrow. These results clearly indicated that the focusing effect can be achieved in the transmission process of keV O− ions through a tapered glass capillary.

The defects on rail surface may have negative effects on the rolling contact fatigue (RCF) performance of the rail materials. This investigation researched on the RCF behaviors of the defected rail materials under various slip ratios and contact stresses. The results show that both the wear loss and the average crack length increase if the slip ratio or the contact stress increases. Moreover, the surface damage characteristics change from simple adhesion spots to significant fatigue crack grouping when slip ratio and contact stress rise. Finally, the experimental results also highlight that the slip ratio provides the deformation area for crack initiation whereas the contact stress accelerates the crack propagation by emphasizing the geometric effects.

Naringin is a major flavanone derivate that has many important biological functions in animals. However, its effect on pigs is unknown. The aim of this study was to investigate the effect of naringin supplementation on performance, carcass traits, meat quality and oxidative stability in finishing pigs. Ninety-six pigs, with an average initial body weight of 66.2 ± 0.63 kg, were randomly divided into four groups. One group was fed a basal diet without supplementation (control), and the three others were fed diets supplemented with 0.5, 1.0 or 1.5 g naringin /kg DM of feed for 50 days. Each treatment was replicated six times with four pigs per replicate. Feed and water were available ad libitum. The 0.5 g/kg naringin treatment group had an improved loin eye muscle area, reduced serum triglycerides and were leaner compared with the other groups. Pigs in the 1.5 g/kg naringin treatment had higher pH45min values and inosine monophosphate concentrations, and lower MyHC IIb mRNA expression in muscle than the other groups. MyHC IIa mRNA expression was significantly up-regulated in all naringin-supplemented diet groups. Naringin significantly increased superoxide dismutase (SOD) activity and total anti-oxidative capacity in meat, as well as SOD and glutathione peroxidase activity in the liver. These results indicate that the dietary addition of naringin at 0.5 g/kg improved carcass characteristics, while 1.5 g/kg improved the oxidative stability and pork quality in finishing pigs. Keywords: antioxidant capacity; carcass characteristics; naringin-supplemented diets; pork quality

Cathelicidins are a major family of antimicrobial peptides (AMPs), an important component of innate immune system, playing a critical role in host defense and disease resistance in virtually all living species. Polymorphism and functional studies on cathelicidin of Tianzhu white yak contribute to understanding the specific innate immune mechanism in animals living at high altitudes in comparison to cattle and domesticated white yak. Thirty-six individuals of Tianzhu white yak, originating from the area of three ecotypes (Gansu in China), were investigated. The total length of the aligned Yak cathelicidin 6 (CATHL-6) sequences was 1923 bp, including six single nucleotide polymorphisms and one indel. Ten haplotypes were identified, and phylogenetic analyses resolved those 10 haplotypes in two clusters. The results indicate that the white yak originated from two domestication sites. In addition, lack of significant pairwise difference between sequences (Tajima's D = 0.92865, P > 0.10) in the CATHL-6 region indicates absence of population size expansion in current white yak population.

Desmoglein 4 (DSG4) has an important role in the development of wool traits in domestic animals. The full-length DSG4 gene, which contains 3918 bp, a complete open-reading-frame, and encodes a 1040-amino acid protein, was amplified from Liaoning cashmere goat. The sequence was compared with that of DSG4 from other animals and the results show that the DSG4 coding region is consistent with interspecies conservation. Thirteen single-nucleotide polymorphisms (SNPs) were identified in a highly variable region of DSG4, and one SNP (M-1, G>T) was significantly correlated with white and black coat color in goat. Haplotype distribution of the highly variable region of DSG4 was assessed in 179 individuals from seven goat breeds to investigate its association with coat color and its differentiation among populations. However, the lack of a signature result indicates DGS4 haplotypes related with the color of goat coat.

The aim of this research was to identify the dynamic diversity of Small Tail Han sheep in its main producing areas between different years, and provide a basis for a breeding and genetic resources conservation strategy. For this purpose, 15 microsatellites were genotyped for Small Tail Han Sheep sampled in 2014 from Heze, China, and a comparative analysis of these data with those from a previous study was undertaken using meta-analysis. The results reveal that inbreeding has caused a reduction in diversity of Small Tail Han Sheep from 2008 to 2014. Overall, our results are helpful in understanding the dynamic change in diversity, as well as providing information for a conservation strategy for this population.

Immune-related miRNAs in breast milk are extracellular miRNAs that are related to immune organ development and regulation of the immune function in infants and young animals. The goal of this study was to compare the expression levels of five immune-related miRNAs in breast milk in black goats, humans, and dairy cattle. The miRNAs from milk were extracted and the expression levels were assessed using quantitive RT-PCR methods. MiR-146, miR-155, miR-181a, miR-223, and miR-150 were all detected in Dazu black goat milk, and these miRNAs were significantly more highly expressed in colostrum than in mature milk of goats (P < 0.01), except for miR-150. Further, all five miRNAs were expressed in human colostrum, but patterns differed from those in goats: miR-146 and miR-155 were highly expressed (P < 0.01) in human colostrum, whereas miR-223 was abundant in goat colostrum (P < 0.01). In addition, five miRNAs were significantly higher in bovine mature milk than in goat milk (P < 0.01). Taken together, these results confirm that immune-related miRNAs are rich in breast milk with different expression levels depending on the lactation phase and species.

The thermal stability and dielectric properties of amorphous CaZrOx film prepared by pulsed laser deposition (PLD) have been investigated. X-ray diffraction (XRD) investigation shows that CaZrOx film still remains amorphous after rapid thermal annealing at 700 °C for 10 min. Differential thermal analysis (DTA) indicates that the crystallization temperature of CaZrOx film is about 729.53 °C, which is significantly higher than that of amorphous ZrO2 films prepared at the similar conditions. High-resolution transmission electron microscopy (HRTEM) and X-ray photon spectroscopy (XPS) analysis reveal there exists a Si-O transition layer between the CaZrOx film and Si substrate. The permittivity of CaZrOx film is about 10.5 (at 1 MHz) by measuring a Pt/CaZrOx/Pt MIM structure. Under the optimized conditions, a small EOT=0.91 nm and a leakage current density of 125 mA/cm2 at 1 V gate voltage were obtained. The enhanced thermal stability and improved electrical characteristics suggest that the amorphous CaZrOx film may be an attractive gate dielectric alternative for next generation MOS field effect transistor applications.