66 results on '"Wurster, M."'
Search Results
2. RADON: rational decomposition and orchestration for serverless computing
- Author
-
Casale, G., Artač, M., Heuvel, W.-J., Hoorn, A., Jakovits, P., Leymann, F., Long, M., Papanikolaou, V., Presenza, D., Russo, A., Srirama, S., Tamburri, D., Wurster, M., and Zhu, L.
- Abstract
Emerging serverless computing technologies, such as function as a service (FaaS), enable developers to virtualize the internal logic of an application, simplifying the management of cloud-native services and allowing cost savings through billing and scaling at the level of individual functions. Serverless computing is therefore rapidly shifting the attention of software vendors to the challenge of developing cloud applications deployable on FaaS platforms. In this vision paper, we present the research agenda of the RADON project (http://radon-h2020.eu), which aims to develop a model-driven DevOps framework for creating and managing applications based on serverless computing. RADON applications will consist of fine-grained and independent microservices that can efficiently and optimally exploit FaaS and container technologies. Our methodology strives to tackle complexity in designing such applications, including the solution of optimal decomposition, the reuse of serverless functions as well as the abstraction and actuation of event processing chains, while avoiding cloud vendor lock-in through models.
- Published
- 2024
- Full Text
- View/download PDF
3. Extracellular degradation of phenol by the cyanobacterium Synechococcus PCC 7002
- Author
-
Wurster, M., Mundt, S., Hammer, E., Schauer, F., and Lindequist, U.
- Published
- 2003
- Full Text
- View/download PDF
4. National experience with an electronic medical record for anticoagulation therapy
- Author
-
Woyach, J. and Wurster, M.
- Published
- 2008
5. RADON: rational decomposition and orchestration for serverless computing
- Author
-
Casale, G., primary, Artač, M., additional, van den Heuvel, W.-J., additional, van Hoorn, A., additional, Jakovits, P., additional, Leymann, F., additional, Long, M., additional, Papanikolaou, V., additional, Presenza, D., additional, Russo, A., additional, Srirama, S. N., additional, Tamburri, D. A., additional, Wurster, M., additional, and Zhu, L., additional
- Published
- 2019
- Full Text
- View/download PDF
6. Rosazea mit eosinophilen Follikulitiden als „presenting sign“ bei einer HIV-infizierten Patientin
- Author
-
Noll, M., additional, Wurster, M., additional, and von den Driesch, P., additional
- Published
- 2017
- Full Text
- View/download PDF
7. A phase 1/2, dose-escalation trial of deferasirox for the treatment of iron overload in HFE-related hereditary hemochromatosis
- Author
-
Phatak, P, Brissot, P, Wurster, M, Adams, Pg, Bonkovsky, Hl, Gross, J, Malfertheiner, P, Mclaren, Gd, Niederau, C, Piperno, A, Powell, Lw, Russo, Mw, Stoelzel, U, Stremmel, W, Griffel, L, Lynch, N, Zhang, Yy, Pietrangelo, Antonello, Hatak, P, Brissot, P, Wurster, M, Adams, P, Bonkovsky, H, Gross, J, Malfertheiner, P, Mclaren, G, Niederau, C, Piperno, A, Powell, L, Russo, M, Stoelzel, U, Stremmel, W, Griffel, L, Lynch, N, Zhang, Y, and Pietrangelo, A
- Subjects
Adult ,Male ,Iron Overload ,Materials science ,Iron absorption ,Medizin ,Iron Chelating Agents ,Benzoates ,Steatohepatitis/Metabolic Liver Disease ,Animal science ,Phlebotomy ,medicine ,Humans ,Aged ,Dose-Response Relationship, Drug ,Hepatology ,Enamel paint ,Transferrin saturation ,Homozygote ,Deferasirox ,Transferrin ,Middle Aged ,Triazoles ,Alternative treatment ,Treatment period ,dose-escalation trial, deferasirox, iron overload, hemochromatosis ,Poor venous access ,Amino Acid Substitution ,Creatinine ,TRANSFUSION-DEPENDENT ANEMIAS ,MYELODYSPLASTIC SYNDROMES ,BETA-THALASSEMIA ,NATURAL-HISTORY ,SERUM FERRITIN ,DEFEROXAMINE ,ICL670 ,CHELATOR ,POPULATION ,EXJADE(R) ,visual_art ,Hereditary hemochromatosis ,Ferritins ,visual_art.visual_art_medium ,Female ,Hemochromatosis ,Safety ,medicine.drug - Abstract
Hereditary hemochromatosis (HH) is characterized by increased intestinal iron absorption that may result in iron overload. Although phlebotomy is widely practiced, it is poorly tolerated or contraindicated in patients with anemias, severe heart disease, or poor venous access, and compliance can vary. The once-daily, oral iron chelator, deferasirox (Exjade) may provide an alternative treatment option. Patients with HH carrying the HFE gene who were homozygous for the Cys282Tyr mutation, serum ferritin levels of 300-2000 ng/mL, transferrin saturation $ge;45%, and no known history of cirrhosis were enrolled in this dose-escalation study to characterize the safety and efficacy of deferasirox, comprising a core and an extension phase (each 24 weeks). Forty-nine patients were enrolled and received starting deferasirox doses of 5 (n = 11), 10 (n = 15), or 15 (n = 23) mg/kg/day. Adverse events were generally dose-dependent, the most common being diarrhea, headache, and nausea (n = 18, n = 10, and n = 8 in the core and n = 1, n = 1, and n = 0 in the extension, respectively). More patients in the 15 mg/kg/day than in the 5 or 10 mg/kg/day cohorts experienced increases in alanine aminotransferase and serum creatinine levels during the 48-week treatment period; six patients had alanine aminotransferase >3× baseline and greater than the upper limit of normal range, and eight patients had serum creatinine >33% above baseline and greater than upper limit of normal on two consecutive occasions. After receiving deferasirox for 48 weeks, median serum ferritin levels decreased by 63.5%, 74.8%, and 74.1% in the 5, 10, and 15 mg/kg/day cohorts, respectively. In all cohorts, median serum ferritin decreased to
- Published
- 2010
8. A Phase I/Ii, Open-Label, Dose-Escalation Trial Using The Once-Daily Oral Chelator Deferasirox To Treat Iron Overload In Hfe-Related Hereditary Hemochromatosis: Final Results
- Author
-
Phatak, P, Brissot, P, Wurster, M, Adams, P, Bonkovsky, H, Gross, J, Malfertheiner, P, Mclaren, G, Niederau, C, Powell, L, Russo, M, Stoelzel, U, Stremmel, W, Griffel, L, Lynch, N, Zhang, Y, Pietrangelo, A., PIPERNO, ALBERTO, Phatak, P, Brissot, P, Wurster, M, Adams, P, Bonkovsky, H, Gross, J, Malfertheiner, P, Mclaren, G, Niederau, C, Piperno, A, Powell, L, Russo, M, Stoelzel, U, Stremmel, W, Griffel, L, Lynch, N, Zhang, Y, and Pietrangelo, A
- Subjects
Dose-Escalation Trial, Oral Chelator Deferasirox, Hfe-Related Hereditary Hemochromatosis, Iron Overload - Published
- 2010
9. An in vitro approach for evaluating the immunotoxic potential of Cannabidiol
- Author
-
Zwicker, P., primary, Schultze, N., additional, Niehs, S., additional, Methling, K., additional, Wurster, M., additional, Bernhardt, J., additional, Drwal, M., additional, Nickel, J., additional, Dunkel, M., additional, Wachlin, G., additional, Lalk, M., additional, Preissner, R., additional, Lindequist, U., additional, and Haertel, B., additional
- Published
- 2015
- Full Text
- View/download PDF
10. Omics technologies for the prediction of immunmodulatory effects
- Author
-
Zwicker, P., primary, Schultze, N., additional, Niehs, S., additional, Methling, K., additional, Wurster, M., additional, Bernhardt, J., additional, Wachlin, G., additional, Lalk, M., additional, Preissner, R., additional, Lindequist, U., additional, and Haertel, B., additional
- Published
- 2015
- Full Text
- View/download PDF
11. Evaluation of the immunotoxic potential of Tulipalin A by a combination of functional in-vitro investigations and omics analyses
- Author
-
Schultze, N., primary, Zwicker, P., additional, Niehs, S., additional, Methling, K., additional, Wurster, M., additional, Bernhardt, J., additional, Drwa, M., additional, Nickel, J., additional, Dunkel, M., additional, Wachlin, G., additional, Lalk, M., additional, Preissner, R., additional, Lindequist, U., additional, and Haertel, B., additional
- Published
- 2015
- Full Text
- View/download PDF
12. Apixaban for extended treatment of venous thromboembolism
- Author
-
Agnelli, G, Buller, H, Cohen, A, Gallus, A, Raskob, G, Weitz, J, Prins, M, Brandjes, D, Kolbach, D, Limburg, M, Mac Gillavry, M, Otten, Jm, Peters, R, Roos, Y, Segers, A, Slagboom, T, Bounameaux, H, Hirsh, J, Samama, Mm, Wedel, H, Curto, M, Johnson, M, Masiukiewicz, U, Pak, R, Porcari, A, Sanders, P, Sisson, M, Sullivan, B, Thompson, J, Auerbach, J, Cesario, L, Gamero, M, Gordon, M, Griffiths, A, Noble, M, Ott, J, Pennington, A, Peffer, A, Reinhold, P, Simmons, M, Urwin, K, Ceresetto, J, Mcrae, S, Pabinger, I, Pereira, Ah, Spencer, F, Gorican, K, Husted, Se, Mottier, D, Harenberg, J, Pinjala, R, Zeltser, D, Imberti, D, Sandset, M, Torbicki, A, Fijalkowska, A, Albino, Jp, Kirienko, A, Shvarts, Y, Monreal, M, Jacobson, B, Dolan, G, Gudz, I, Ortel, T, Spyropoulos, A, Skupyy, O, Beryer Westendorf, J, De Pellegrin, A, Prasol, V, Schellong, S, Falvo, N, Abramov, I, Cizek, V, Husted, S, Desai, S, Barillari, G, Sergeev, O, Chetter, I, Inbal, A, Mccollum, C, Shvalb, P, Torp Pedersen, C, Vasylyuk, S, Kraemmer Nielsen, H, Pernod, G, Schmidt, J, Bova, C, Gerasymov, V, Pabinger Fasching, I, Skalicka, L, Zaichuk, A, Achkar, A, Bremmelgaard, A, Chochola, J, Gould, T, Khalafallah, A, Jakobsen, T, Rose, P, Zhukov, B, Dedek, V, Mirete Ferrer, J, Pesant, Y, Repin, A, Salem, H, Solis Morales, L, Spacek, R, Cannon, K, Grzelakowski, P, Jindal, R, Pereira, A, Zidkova, E, Ambrosio, G, Cardozo, M, Dunaj, M, Gavish, D, Ghanima, W, Leduc, Jj, Mismetti, P, Panico, M, Porreca, E, Riera, A, Bareford, D, Chong, B, Dvoryashina, I, Gómez Cerezo, J, Kobza, I, Nielsen, T, Pendleton, R, Pullman, J, Schiffman, G, Stanbro, M, Zwettler, U, Aquilanti, S, Bratsch, H, Cohen, K, Elias, D, Gan, E, Holaj, R, Klinke, W, Liu, Hs, Sandset, Pm, van Nieuwenhuizen, E, Álvarez Sala LA, Basson, M, Braester, A, Bura Riviere, A, Calvo Vargas, C, Correa, J, Elias, M, Frost, L, Landolfi, R, Marschang, P, Moreira, R, Natarajan, S, Pottier, P, Tosetto, A, Tuxen, C, Vöhringer, Hf, Alexander, A, Barbarash, O, Fajardo Campos, P, Graham, M, Gubka, O, Hudcovic, M, Hussein, O, Jackson, D, Katelnitskiy, I, Lawall, H, Palareti, G, Poggio, R, Roos, J, Simonneau, G, Smith, Sw, Szopinski, P, Zimlichman, R, Bridgers, D, Colan, D, Czekalski, P, De Jong, D, Fortinez, Jt, Garcia Bragado, F, Harrington, D, Izbicki, G, Kadr, H, Koslow, A, Loftus, I, Marais, H, Neumeister, A, Oliven, A, Palla, A, Pop, C, Prandoni, Paolo, Puskas, A, Sanchez Llamas, F, Shotan, A, Singh, P, Tveit, A, Baker, R, Borja, V, Brenner, B, Brown, H, Cha, Tj, Cohen, Y, D'Angelo, A, Dhar, A, Friis, E, Hueur, H, Jiménez Rodríguez Madridejos, R, Karl, J, Karrasch, J, Lishner, M, Manenti, E, Meneveau, N, Nguyen, D, Sanchez Escalante, L, Santoscoy Ibarra, J, Sokurenko, G, Staroverov, I, Stein, R, Abdullah, I, Alcocer Gamba, M, Balanda, J, Bruckner, I, Calabuig Alborch, J, Caraco, Y, Comerota, A, Cromer, M, de Araujo Filho, J, De los Rios Ibarra, M, Diaz Castañon, J, Doshi, A, Ebrahim, I, Fessel, Wj, Fletcher, E, Fourie, N, Fu, C, Gutowski, P, Haddad, G, Hoffman, U, Jardula, M, Kvasnicka, T, Lewczuk, J, Leyden, M, Livneh, A, Lodigiani, C, Lovell, C, Miekus, P, Paloma, Mj, Parakh, R, Raval, M, Schmidt Lucke, J, Shtutin, O, Soroka, V, Stevens, D, Sulik, P, Tay, Jc, Vejby Christensen, H, Vinereanu, D, Baghestanian, M, Bono, J, Cerana, S, Freire, A, Gibson, K, Giumelli, C, Iastrebner, C, Karpenko, A, Kelly, A, Lacroix, P, Lafata, J, Lobo, S, Macik, Bg, Marchena Yglesias, P, Nishinari, K, Podczeck Schweighofer, A, Raby, K, Sirpal, S, Solymoss, S, van Zyl, L, Vargas Núñez JA, von Bilderling, P, Warr, T, Wronski, J, Wurster, M, Albino, Ja, Albuquerque, L, Averill, F, Baek, Sh, Bello, F, Bergoeing, M, Blanc, Fx, Bloomberg, R, Bolster, D, Brockmyre, A, Calimano, C, Checketts, D, Cieplinski, W, Chervu, A, Collado, F, Denaro, C, Gaciong, Z, Game, M, Iskander, A, Kaatz, S, Kim, Di, Koura, F, Laguna, F, Lanas Zanetti, F, Lindhoff Last, E, Melaniuk, M, Meade, A, Murphy, T, Ng, Hj, Páramo Fernández JA, Patil, C, Piovella, F, Prisco, D, Pruszczyk, P, Reimers, G, Rivera, E, Rodriguez Cintron, W, Rosenthal, S, Salbach, P, Salvador, D, Schuller, D, Siragusa, S, Staniszewski, R, Torp, R, Vora, K, Yip, G, Alfieri, A, Belaji, V, Bhagavan, N, Carnovali, M, Cobos Segarra, J, Di Todaro, F, Dowell, A, Corder, C, Crispin, P, Cuadrado, J, Flippo, G, Fraiz, J, Guillaumon, A, Gvora, T, Hakki, S, Harris, L, Ison, R, Htun, Pt, Jasani, R, Kates, M, Kaminski, L, Kamerkar, D, Kroger, K, Laperna, L, Leiva, J, Luber, J, Mccann, A, Mckenzie, W, Menna Barreto, S, Moran, J, Nikulnikov, P, Paliwal, Y, Patel, M, Pilger, E, Renwick, W, Shevela, A, Starosiliz, D, Stringam, S, To, R, Updegrove, J, Van Bellen, B, Waintrub, M, White, J, Yeo, E, Zangroniz, P, Zeltser, D., ACS - Amsterdam Cardiovascular Sciences, Vascular Medicine, APH - Amsterdam Public Health, Cardiology, ANS - Amsterdam Neuroscience, Neurology, Department of Vascular Medicine (DVM - AMC), Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (AMC), University of Amsterdam [Amsterdam] (UvA)-University of Amsterdam [Amsterdam] (UvA), Thrombosis and Atherosclerosis Research Institute (TARI), McMaster University [Hamilton, Ontario], Centre d'Investigation Clinique (CIC - Brest), Université de Brest (UBO)-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe d'Etude de la Thrombose de Bretagne Occidentale (GETBO), Université de Brest (UBO)-Institut Brestois Santé Agro Matière (IBSAM), and Université de Brest (UBO)-Université de Brest (UBO)
- Subjects
Male ,Kaplan-Meier Estimate ,030204 cardiovascular system & hematology ,Placebo group ,DISEASE ,law.invention ,0302 clinical medicine ,Randomized controlled trial ,law ,Recurrence ,Fibrinolytic agents ,030212 general & internal medicine ,IDRAPARINUX ,Administration of drugs ,Follow up studies ,food and beverages ,General Medicine ,Venous Thromboembolism ,Middle Aged ,3. Good health ,Intention to Treat Analysis ,Treatment Outcome ,Treatment dose ,Anesthesia ,Creatinine ,Factor Xa ,Fibrinolítics ,Apixaban ,Female ,Administració de medicaments ,Major bleeding ,medicine.drug ,ARTERIAL CARDIOVASCULAR EVENTS ,INTENSITY WARFARIN THERAPY ,PULMONARY-EMBOLISM ,LONG-TERM ,PREVENTION ,Adult ,Pyridones ,Hemorrhage ,03 medical and health sciences ,Double-Blind Method ,Fibrinolytic Agents ,Thromboembolism ,medicine ,Humans ,Tromboembolisme ,Aged ,Intention-to-treat analysis ,business.industry ,fungi ,Pyrazoles ,business ,Venous thromboembolism ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Factor Xa Inhibitors ,Follow-Up Studies - Abstract
International audience; Background Apixaban, an oral factor Xa inhibitor that can be administered in a simple, fixed-dose regimen, may be an option for the extended treatment of venous thromboembolism. Methods In this randomized, double-blind study, we compared two doses of apixaban (2.5 mg and 5 mg, twice daily) with placebo in patients with venous thromboembolism who had completed 6 to 12 months of anticoagulation therapy and for whom there was clinical equipoise regarding the continuation or cessation of anticoagulation therapy. The study drugs were administered for 12 months. Results A total of 2486 patients underwent randomization, of whom 2482 were included in the intention-to-treat analyses. Symptomatic recurrent venous thromboembolism or death from venous thromboembolism occurred in 73 of the 829 patients (8.8%) who were receiving placebo, as compared with 14 of the 840 patients (1.7%) who were receiving 2.5 mg of apixaban (a difference of 7.2 percentage points; 95% confidence interval [CI], 5.0 to 9.3) and 14 of the 813 patients (1.7%) who were receiving 5 mg of apixaban (a difference of 7.0 percentage points; 95% CI, 4.9 to 9.1) (P
- Published
- 2012
13. Proximale Tibiafraktur nach eingeheilter Vorderer Kreuzbandersatzplastik, der tibiale Tunnel ein Risiko?
- Author
-
Pätzold, R, Wurster, M, Augat, P, Gonschorek, O, and Bühren, V
- Subjects
ddc: 610 ,Tunnelwidening ,610 Medical sciences ,Medicine ,VKB-Ersatz ,Tibiakopffraktur - Abstract
Fragestellung: Bei der vorderern Kreuzbandersatzplastik wird durch die tibiale Bohrung eine Schwächung der starken medialen Kondyle erzeugt [ref:1]. Zusätzlich verändert sich die Bohrung im Laufe der Zeit (Tunnelwidening und Resorptionslysen der resorbierbaren Schrauben) [ref:2].[for full text, please go to the a.m. URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2012)
- Published
- 2012
- Full Text
- View/download PDF
14. Welchen Einfluss haben Begleitverletzungen bei proximalen bikondylären Tibiafrakturen (AO Typ C) auf Behandlung und Outcome?
- Author
-
Wurster, M., Pätzold, R., Gonschorek, O., and Bühren, V.
- Subjects
ddc: 610 ,610 Medical sciences ,Medicine - Abstract
Fragestellung: Proximale Tibiafrakturen treten häufig mit anderen versorgungspflichtigen Verletzungen auf. Den derzeit gängigen Studien liegen Patienten mit und ohne Begleitverletzungen als Gesamtkollektiv zugrunde. Unsere Frage ist: Welchen Einfluss haben Begleitverletzungen auf Behandlung[for full text, please go to the a.m. URL], Deutscher Kongress für Orthopädie und Unfallchirurgie; 74. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 96. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 51. Tagung des Berufsverbandes der Fachärzte für Orthopädie
- Published
- 2010
- Full Text
- View/download PDF
15. Die zunehmende Bedeutung der unikondylären proximalen Tibiafraktur (AO Typ B) im Skisport: Erste Ergebnisse einer prospektiven Studie
- Author
-
Pätzold, R., Wurster, M., Gutsfeld, P., Gonschorek, O., and Bühren, V.
- Subjects
ddc: 610 ,610 Medical sciences ,Medicine - Abstract
Fragestellung: Seit der Einführung der Carvingski ist eine Abnahme der Gesamtverletzungszahlen bei gleichzeitiger Zunahme der Knieverletzungen zu beobachten. Bis heute sind bei proximalen Tibiafrakturen im Skisport nur Einzelfälle beschrieben. Welche Inzidenz und Ergebnisse haben unikondyläre[for full text, please go to the a.m. URL], Deutscher Kongress für Orthopädie und Unfallchirurgie; 74. Jahrestagung der Deutschen Gesellschaft für Unfallchirurgie, 96. Tagung der Deutschen Gesellschaft für Orthopädie und Orthopädische Chirurgie, 51. Tagung des Berufsverbandes der Fachärzte für Orthopädie
- Published
- 2010
16. Cytotoxic activity of aquatic fungus Pseudohalonectria lignicola on two different human cell lines
- Author
-
Schneider, S, primary, Srirangan, N, additional, Wurster, M, additional, Lindequist, U, additional, and Lalk, M, additional
- Published
- 2014
- Full Text
- View/download PDF
17. Expression of estrogen receptor ß- isoforms in invasive breast cancer cell lines
- Author
-
Petzke, N, Seeger, H, Wurster, M, Fehm, T, Schuetz, C, Solomayer, E, Wallwiener, D, and Neubauer, H
- Subjects
ddc: 610 - Published
- 2006
18. Proximale Tibiafrakturen im Skisport – Inzidenz und Risikofaktoren
- Author
-
Pätzold, R., additional, Spiegl, U., additional, Wurster, M., additional, Augat, P., additional, Gutsfeld, P., additional, Gonschorek, O., additional, and Bühren, V., additional
- Published
- 2013
- Full Text
- View/download PDF
19. 187 A PHASE I/II, OPEN-LABEL, DOSE-ESCALATION TRIAL USING THE ONCE-DAILY ORAL CHELATOR DEFERASIROX TO TREAT IRON OVERLOAD IN HFE-RELATED HEREDITARY HEMOCHROMATOSIS: FINAL RESULTS
- Author
-
Phatak, P., primary, Brissot, P., additional, Wurster, M., additional, Adams, P.C., additional, Bonkovsky, H.L., additional, Gross, J., additional, Malfertheiner, P., additional, McLaren, G.D., additional, Niederau, C., additional, Piperno, A., additional, Powell, L.W., additional, Russo, M., additional, Stoelzel, U., additional, Stremmel, W., additional, Griffel, L., additional, Lynch, N., additional, Zhang, Y., additional, and Pietrangelo, A., additional
- Published
- 2010
- Full Text
- View/download PDF
20. Prädiktive Bedeutung des Östrogenrezeptors β bei neoadjuvant behandelten Mammakarzinom-Patientinnen
- Author
-
Ruoff, A, primary, Wurster, M, additional, Meisner, C, additional, Vogel, U, additional, Juhasz-Böss, I, additional, Solomayer, EF, additional, Wallwiener, D, additional, Fehm, T, additional, and Neubauer, H, additional
- Published
- 2009
- Full Text
- View/download PDF
21. National experience with an electronic medical record for anticoagulation therapy
- Author
-
Woyach, J., primary and Wurster, M., additional
- Published
- 2007
- Full Text
- View/download PDF
22. Korrelation von Expressionsprofilen verschiedener Östrogenrezeptor-β-Isoformen mit der Migration von Mammakarzinomzellen
- Author
-
Neubauer, H, primary, Wurster, M, additional, Schütz, C, additional, Seeger, H, additional, Wallwiener, D, additional, Solomayer, E, additional, and Fehm, T, additional
- Published
- 2006
- Full Text
- View/download PDF
23. Antimicrobial, antioxidant and cytotoxic activities of selected medicinal plants from Yemen
- Author
-
Al-Fatimi, M, primary, Wurster, M, additional, Schröder, G, additional, and Lindequist, U, additional
- Published
- 2006
- Full Text
- View/download PDF
24. High Voltage Testing of GIS: Application of Frequency Tuned Resonant Test Sets and Return of Experience from On-site Tests
- Author
-
Schichler, U., primary and Wurster, M., additional
- Published
- 2006
- Full Text
- View/download PDF
25. Interventionell-radiologischer Therapieansatz zur Behandlung der Carney Triade
- Author
-
Lehnert, T, primary, Wurster, M, additional, Mack, M, additional, Balzer, JO, additional, Thalhammer, A, additional, and Vogl, TJ, additional
- Published
- 2005
- Full Text
- View/download PDF
26. Interventionell-Radiologischer Therapieansatz zur Behandlung der Carney-Triade
- Author
-
Lehnert, T, primary, Wurster, M, additional, Wetter, A, additional, Eichler, K, additional, Mack, MG, additional, and Vogl, TJ, additional
- Published
- 2004
- Full Text
- View/download PDF
27. Malignancy-induced lactic acidosis.
- Author
-
DOOLITTLE, GARY C., WURSTER, MARK W., ROSENFELD, CRAIG S., BODENSTEINER, DAVID C., Doolittle, G C, Wurster, M W, Rosenfeld, C S, and Bodensteiner, D C
- Published
- 1988
- Full Text
- View/download PDF
28. Extracellular degradation of phenol by the cyanobacterium SynechococcusPCC 7002
- Author
-
Wurster, M., Mundt, S., Hammer, E., Schauer, F., and Lindequist, U.
- Abstract
Investigations of the unicellular marine cyanobacterium SynechococcusPCC 7002 revealed its ability to metabolize phenol under non-photosynthetic conditions up to 100 mg L−1. Under continuous light, photoautotrophic growth was reduced only slightly in the presence of this phenol concentration, but no transformation was observed. However neither under photoautotrophic nor heterotrophic conditions were the cells able to use phenol for growth. During the degradation of phenol in the dark cis,cis-muconic acid was produced as the major product, which was identified by gas chromatography/mass spectrometry. This result was confirmed by an identical absorption spectrum and an identical retention time in high performance liquid chromatographic analysis with authentic muconic acid as standard. This provides the first record for an ortho-fission of a phenolic substance by cyanobacteria. Further investigations of the breakdown mechanism of phenol have shown that the transformation is an extracellular process inhibited by heat, proteases and metal ions and is probably catalyzed by a protein.
- Published
- 2003
- Full Text
- View/download PDF
29. Evaluation of Cool Season Grass Species and Varieties Using In Vivoand In VitroTechniques1
- Author
-
Wurster, M. J., Kamstra, L. D., and Ross, J. G.
- Abstract
Digestibility of the cool season grass species, smooth bromegrass (Bromus inermisLeyss), intermediate wheatgrass [Agropyron intermedium(Host) Beauv.] and the crested wheatgrass complex [Agropyron sibericum(Willd.) Beauv. and Agropyron desertorum(Fisch.) Shult.] was measured at heading and 2 weeks after by in vivoand in vitrotechniques. In addition, plant fraction percentages and digestibilities were determined throughout the season for two varieties within each of the above species, ‘Manchar’ and ‘Sac’ bromegrass, ‘Greenar’ and ‘Oahe’ intermediate wheatgrass, and ‘P‐27’ Siberian wheatgrass and ‘Nordan’ crested wheatgrass. Both in vivoand in vitrocriteria, as well as chemical analyses, indicated that intermediate wheatgrass was lower in digestibility than the other two species. Bromegrass and crested wheatgrass differed very little; bromegrass was slightly superior under in vivotest, while crested wheatgrass was slightly but significantly superior when in vitrotechniques were used. A high correlation (r=0.89) was found between in vitroand in vivodry matter digestibility. At heading and 2 weeks after, in vitrodry matter digestibility indicated that Greenar was significantly superior to Oahe. No significant difference was found between the varieties of the other species. When entire plant and plant fractions were sampled over the growing season, Sac bromegrass was significantly superior to Manchar, Greenar intermediate wheatgrass to Oahe and Nordan crested wheatgrass to Siberian. These results indicate the possibility of selecting for more highly digestible stems and sheaths to produce a variety for utilization at the time of maximumd dry matter yield. For grazing purposes, however, selection at an early stage for whole plant digestibility would seem desirable.
- Published
- 1971
- Full Text
- View/download PDF
30. Evaluation of ERalpha, PR and ERbeta isoforms in neoadjuvant treated breast cancer
- Author
-
Wurster M, Ruoff A, Meisner C, Seeger H, Vogel U, Juhasz-Böss I, Solomayer E, Wallwiener D, Tanja Fehm, and Neubauer H
31. Antimicrobial, cytotoxic and antioxidant activity of selected basidiomycetes from Yemen
- Author
-
Mohamed Al-Fatimi, Wurster M, Kreisel H, and Lindequist U
- Subjects
Antibiotics, Antineoplastic ,Yemen ,Picrates ,Basidiomycota ,Biphenyl Compounds ,Solvents ,Humans ,Microbial Sensitivity Tests ,Drug Screening Assays, Antitumor ,Antioxidants ,Anti-Bacterial Agents - Abstract
Dichloromethane, methanol and aqueous extracts of 23 selected Basidiomycetes species fruiting bodies collected in Yemen were screened in vitro for their antibacterial activities against three Gram-positive bacteria (Staphyloccocus aureus, Bacillus subtilis, Micrococcus flavus), two Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) and against one yeast fungus (Candida maltosa), as well as for their cytotoxic and antioxidant activity. The highest antibacterial activity was shown by extracts from Agaricus sp. (Type 1), Coriolopsis caperata, Ganoderma colossus, Ganoderma resinaceum, Phellorinia herculea and Tulostoma obesum. Strong antioxidative effects employing the DPPH assay were exhibited by methanol extracts from Ganoderma resinaceum, Inonotus ochroporus, Phellinus rimosus and Phellorinia herculea. The results provide evidence that some of the studied fungi might be potential sources for new biologically active agents.
32. Evaluation of Cool Season Grass Species and Varieties Using In Vivo and In Vitro Techniques 1
- Author
-
Wurster, M. J., primary, Kamstra, L. D., additional, and Ross, J. G., additional
- Published
- 1971
- Full Text
- View/download PDF
33. Light emission measurements of predischarges in insulating oil
- Author
-
Badent, R., primary, Kist, K., additional, Schwab, A.J., additional, and Wurster, M., additional
- Full Text
- View/download PDF
34. Light emission measurements of predischarges in insulating oil.
- Author
-
Badent, R., Kist, K., Schwab, A.J., and Wurster, M.
- Published
- 1998
- Full Text
- View/download PDF
35. Clostridioides difficile Modifies its Aromatic Compound Metabolism in Response to Amidochelocardin-Induced Membrane Stress.
- Author
-
Brauer M, Hotop SK, Wurster M, Herrmann J, Miethke M, Schlüter R, Dittmann S, Zühlke D, Brönstrup M, Lalk M, Müller R, Sievers S, Bernhardt J, and Riedel K
- Subjects
- Clostridioides, Gram-Negative Bacteria, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Gram-Positive Bacteria, Proteome, Tetracyclines pharmacology, Phenazines pharmacology, Clostridioides difficile
- Abstract
Amidochelocardin is a broad-spectrum antibiotic with activity against many Gram-positive and Gram-negative bacteria. According to recent data, the antibiotic effect of this atypical tetracycline is directed against the cytoplasmic membrane, which is associated with the dissipation of the membrane potential. Here, we investigated the effect of amidochelocardin on the proteome of Clostridioides difficile to gain insight into the membrane stress physiology of this important anaerobic pathogen. For the first time, the membrane-directed action of amidochelocardin was confirmed in an anaerobic pathogen. More importantly, our results revealed that aromatic compounds potentially play an important role in C. difficile upon dissipation of its membrane potential. More precisely, a simultaneously increased production of enzymes required for the synthesis of chorismate and two putative phenazine biosynthesis proteins point to the production of a hitherto unknown compound in response to membrane depolarization. Finally, increased levels of the ClnAB efflux system and its transcriptional regulator ClnR were found, which were previously found in response to cationic antimicrobial peptides like LL-37. Therefore, our data provide a starting point for a more detailed understanding of C. difficile 's way to counteract membrane-active compounds. IMPORTANCE C. difficile is an important anaerobe pathogen causing mild to severe infections of the gastrointestinal tract. To avoid relapse of the infection following antibiotic therapy, antibiotics are needed that efficiently eradicate C. difficile from the intestinal tract. Since C. difficile was shown to be substantially sensitive to membrane-active antibiotics, it has been proposed that membrane-active antibiotics might be promising for the therapy of C. difficile infections. Therefore, we studied the response of C. difficile to amidochelocardin, a membrane-active antibiotic dissipating the membrane potential. Interestingly, C. difficile 's response to amidochelocardin indicates a role of aromatic metabolites in mediating stress caused by dissipation of the membrane potential.
- Published
- 2022
- Full Text
- View/download PDF
36. An Innovative Protocol for Metaproteomic Analyses of Microbial Pathogens in Cystic Fibrosis Sputum.
- Author
-
Graf AC, Striesow J, Pané-Farré J, Sura T, Wurster M, Lalk M, Pieper DH, Becher D, Kahl BC, and Riedel K
- Subjects
- Bacteria genetics, Humans, Lung, Sputum, Cystic Fibrosis complications, Microbiota
- Abstract
Hallmarks of cystic fibrosis (CF) are increased viscosity of mucus and impaired mucociliary clearance within the airways due to mutations of the cystic fibrosis conductance regulator gene. This facilitates the colonization of the lung by microbial pathogens and the concomitant establishment of chronic infections leading to tissue damage, reduced lung function, and decreased life expectancy. Although the interplay between key CF pathogens plays a major role during disease progression, the pathophysiology of the microbial community in CF lungs remains poorly understood. Particular challenges in the analysis of the microbial population present in CF sputum is (I) the inhomogeneous, viscous, and slimy consistence of CF sputum, and (II) the high number of human proteins masking comparably low abundant microbial proteins. To address these challenges, we used 21 CF sputum samples to develop a reliable, reproducible and widely applicable protocol for sputum processing, microbial enrichment, cell disruption, protein extraction and subsequent metaproteomic analyses. As a proof of concept, we selected three sputum samples for detailed metaproteome analyses and complemented and validated metaproteome data by 16S sequencing, metabolomic as well as microscopic analyses. Applying our protocol, the number of bacterial proteins/protein groups increased from 199-425 to 392-868 in enriched samples compared to nonenriched controls. These early microbial metaproteome data suggest that the arginine deiminase pathway and multiple proteases and peptidases identified from various bacterial genera could so far be underappreciated in their contribution to the CF pathophysiology. By providing a standardized and effective protocol for sputum processing and microbial enrichment, our study represents an important basis for future studies investigating the physiology of microbial pathogens in CF in vivo - an important prerequisite for the development of novel antimicrobial therapies to combat chronic recurrent airway infection in CF., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Graf, Striesow, Pané-Farré, Sura, Wurster, Lalk, Pieper, Becher, Kahl and Riedel.)
- Published
- 2021
- Full Text
- View/download PDF
37. Differential effects of Helenalin, an anti-inflammatory sesquiterpene lactone, on the proteome, metabolome and the oxidative stress response in several immune cell types.
- Author
-
Zwicker P, Schultze N, Niehs S, Albrecht D, Methling K, Wurster M, Wachlin G, Lalk M, Lindequist U, and Haertel B
- Subjects
- Apoptosis drug effects, Carbohydrate Metabolism drug effects, Cell Line, Tumor, Cell Survival drug effects, Cytokines metabolism, Humans, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear metabolism, Metabolome drug effects, Oxidative Stress drug effects, Proteome metabolism, Reactive Oxygen Species metabolism, Sesquiterpenes, Guaiane, Anti-Inflammatory Agents pharmacology, Sesquiterpenes pharmacology
- Abstract
Extracts of Arnica spp. are traditionally used due to their anti-inflammatory effects for the topical treatment of e.g. haematoma or muscle distortions. One of the main active compounds is Helenalin, a sesquiterpene lactone that can be found in various Asteraceae. However, immunotoxic effects of the compound are only poorly analysed. In this study, a 2D gel electrophoresis based proteomic approach together with a membrane based proteomic assay, metabolomics and the detection of intracellular reactive oxygen species (iROS) were used to investigate potential immunotoxic properties of Helenalin on the human immune cell lines Jurkat and THP-1 and on human peripheral blood mononuclear cells (PBMC). The study revealed a dose-dependent cytotoxicity towards both tested cell lines and the PBMC. However, the cell lines were less sensitive to the Helenalin treatment than the PBMC. The proteomic assays showed strong effects on the carbohydrate metabolism and the protein folding in THP-1 cells but only weak impact on Jurkat cells. Metabolomic studies as well as iROS detection in THP-1 cells verified the results of the proteomic analysis. In summary, the approaches used in this study were able to identify target pathways of Helenalin especially in THP-1 monocytes and thus enable a risk assessment of the substance., (Copyright © 2016. Published by Elsevier Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
38. A proteomic approach for the identification of immunotoxic properties of Tulipalin A.
- Author
-
Zwicker P, Schultze N, Niehs S, Methling K, Wurster M, Albrecht D, Bernhardt J, Wachlin G, Lalk M, Lindequist U, and Haertel B
- Subjects
- 4-Butyrolactone immunology, 4-Butyrolactone toxicity, Apoptosis drug effects, Cell Cycle drug effects, Cell Survival drug effects, DNA Repair drug effects, Dermatitis, Allergic Contact etiology, Electrophoresis, Gel, Two-Dimensional, Humans, Jurkat Cells drug effects, Jurkat Cells immunology, Jurkat Cells metabolism, Metabolome, Protein Folding drug effects, Purines biosynthesis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Toxicity Tests methods, 4-Butyrolactone analogs & derivatives, Proteomics methods
- Abstract
The immune system is permanently exposed to several environmental influences that can have adverse effects on immune cells or organs leading to immunosuppression or inappropriate immunostimulation, called direct immunotoxicity. The natural compound Tulipalin A (TUPA), a lactone with α-methylene-γ-butyrolactone moiety, can influence the immune system and lead to allergic contact dermatitis. This in vitro study focused on effects of TUPA using two immune cell lines (Jurkat T cells and THP-1 monocytes). To evaluate the immunotoxic potential of the compound, a proteomic approach applying 2D gel electrophoresis and MALDI-TOF/TOF-MS in combination with metabolomic analysis was used after exposure of the cells to IC
10 of TUPA. THP-1 cells showed a strong robustness to TUPA treatment since only five proteins were altered. In contrast, in Jurkat T cells an increase in the abundance of 66 proteins and a decrease of six proteins was determined. These intracellular proteins were mapped to biological processes. Especially an accumulation of chaperones and an influence on the purine synthesis were observed. The changes in purine synthesis were confirmed by metabolomic analysis. In conclusion, the data indicate possible target processes of low doses of TUPA in Jurkat T cells and provides knowledge of how TUPA affects the functionality of immune cells., (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)- Published
- 2016
- Full Text
- View/download PDF
39. A one-stage cultivation process for lipid- and carbohydrate-rich biomass of Scenedesmus obtusiusculus based on artificial and natural water sources.
- Author
-
Schulze C, Reinhardt J, Wurster M, Ortiz-Tena JG, Sieber V, and Mundt S
- Subjects
- Carbohydrates chemistry, Culture Media chemistry, Fatty Acids chemistry, Lipids chemistry, Microalgae metabolism, Nitrates chemistry, Oleic Acid chemistry, Phosphates chemistry, Seawater, Stearic Acids chemistry, Biofuels, Biomass, Scenedesmus metabolism, Water metabolism
- Abstract
A one-stage cultivation process of the microalgae Scenedesmus obtusiusculus with medium based on natural water sources was developed to enhance lipids and carbohydrates. A medium based on artificial sea water, Baltic Sea water and river water with optimized nutrient concentrations compared to the standard BG-11 for nitrate (-75%), phosphate and iron (-90%) was used for cultivation. Although nitrate exhaustion over cultivation resulted in nitrate limitation, growth of the microalgae was not reduced. The lipid content increased from 6.0% to 19.9%, an increase in oleic and stearic acid was observed. The unsaponifiable matter of the lipid fraction was reduced from 19.5% to 11.4%. The carbohydrate yield rose from 45% to 50% and the protein content decreased from 32.4% to 15.9%. Using natural water sources with optimized nutrient concentrations could open the opportunity to modulate biomass composition and to reduce the cultivation costs., (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
40. Chemical Composition, Antimicrobial and Antioxidant Activities of the Volatile Oil of Ganoderma pfeifferi Bres.
- Author
-
Al-Fatimi M, Wurster M, and Lindequist U
- Abstract
In a first study of the volatile oil of the mushroom basidiomycete Ganoderma pfeifferi Bres., the chemical composition and antimicrobial and antioxidant activities of the oil were investigated. The volatile oil was obtained from the fresh fruiting bodies of Ganoderma pfeifferi Bres. By hydrodistillation extraction and analyzed by GC-MS. The antimicrobial activity of the oil was evaluated against five bacteria strains and two types of fungi strains, using disc diffusion and broth microdilution methods. In addition, the antioxidant activity of the oil was determined using DPPH assay. Four volatile compounds representing 90.5% of the total oil were identified. The majority of the essential oil was dominated by 1-octen-3-ol (amyl vinyl carbinol) 1 (73.6%) followed by 1-octen-3-ol acetate 2 (12.4%), phenylacetaldehyde 3 (3.0%) and 6-camphenol 4 (1.5%). The results showed that the Gram-positive bacteria species are more sensitive to the essential oil than Gram-negative bacteria. The oil showed strong antimicrobial activity against Staphylococcus aureus as well as Candida albicans . Moreover, the oil exhibited strong radical scavenging activity in the DPPH assay. This first report on the chemical composition and biological properties of G. pfeifferi volatile oil makes its pharmaceutical uses rational and provides a basis in the biological and phytochemical investigations of the volatile oils of Ganodermataceae species.
- Published
- 2016
- Full Text
- View/download PDF
41. Cylindrofridins A-C, Linear Cylindrocyclophane-Related Alkylresorcinols from the Cyanobacterium Cylindrospermum stagnale.
- Author
-
Preisitsch M, Niedermeyer TH, Heiden SE, Neidhardt I, Kumpfmüller J, Wurster M, Harmrolfs K, Wiesner C, Enke H, Müller R, and Mundt S
- Subjects
- Methicillin-Resistant Staphylococcus aureus drug effects, Microbial Sensitivity Tests, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Resorcinols chemistry, Resorcinols pharmacology, Streptococcus pneumoniae drug effects, Structure-Activity Relationship, Cyanobacteria chemistry, Resorcinols isolation & purification
- Abstract
A rapid and exhaustive one-step biomass extraction as well as an enrichment and cleanup procedure has been developed for HPLC-UV detection and quantification of closely related [7.7]paracyclophanes and structural derivatives based on a two-phase solvent system. The procedure has been validated using the biomass of the carbamidocyclophane- and cylindrocyclophane-producing cyanobacterium Nostoc sp. CAVN2 and was utilized to perform a screening comprising 102 cyanobacterial strains. As a result, three new cylindrocyclophane-related alkylresorcinols, cylindrofridins A-C (1-3), and known cylindrocyclophanes (4-6) were detected and isolated from Cylindrospermum stagnale PCC 7417. Structures of 1-3 were elucidated by a combination of 1D and 2D NMR experiments, HRMS, and ECD spectroscopy. Cylindrofridin A (1) is the first naturally occurring [7.7]paracyclophane-related monomeric derivative. In contrast, cylindrofridins B (2) and C (3) represent dimers related to 1. Due to chlorination at the alkyl carbon atom in 1-3, the site of [7.7]paracyclophane macrocycle formation, the cylindrofridins represent linearized congeners of the cylindrocyclophanes. Compounds 1-3 were not toxic against nontumorigenic HaCaT cells (IC50 values >25 μM) compared to the respective cylindrocyclophanes, but 1 was the only cylindrofridin showing moderate activity against methicillin-resistant Staphylococcus aureus (MRSA) and Streptococcus pneumoniae with MIC values of 9 and 17 μM, respectively.
- Published
- 2016
- Full Text
- View/download PDF
42. Cytotoxic saponins from the seeds of Pittosporum angustifolium.
- Author
-
Bäcker C, Jenett-Siems K, Siems K, Wurster M, Bodtke A, and Lindequist U
- Subjects
- Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Antineoplastic Agents, Phytogenic pharmacology, Carbohydrate Conformation, Cell Line, Tumor, Chromatography, High Pressure Liquid, Drug Screening Assays, Antitumor, Humans, Plant Extracts chemistry, Saponins chemistry, Saponins pharmacology, Spectrometry, Mass, Electrospray Ionization, Spectrophotometry, Infrared, Plant Extracts pharmacology, Rosales embryology, Saponins isolation & purification, Seeds chemistry
- Abstract
Three new acylated R1-barrigenol triterpene glycosides, 1-3, were isolated from the seeds of Pittosporum angustifolium Lodd. together with four known glycosides, 4-7, containing R1- and A1-barrigenol backbones. On the basis of spectroscopic, spectrometric, and chemical analyses the novel compounds were named pittangretosides N-P and established as 21beta-acetoxy-22alpha-angeloyloxy- (1), 21beta-acetoxy-22alpha-(2-acetoxy-2-methylbutyroyloxy)- (2), and 21beta-(2-methylbutyroyloxy)-22alpha-acetoxy-3beta-[beta-D-glucopyranosyl- (1 --> 2)]-[alpha-L-arabinopyranosyl-(1 --> 3)]-[alpha-L-arabinofuranosyl-(1 --> 4)]-beta-D-glucuronopyranosyloxyolean-12-ene-15alpha, 6alpha, 28-triol (3). Evaluation of the in vitro cytotoxicity against three tumour cell lines and one non-tumourigenic cell line revealed antiproliferative effects with IC50 values in a range of 1.74-34.1 microM.
- Published
- 2014
- Full Text
- View/download PDF
43. STABLE results: warfarin home monitoring achieves excellent INR control.
- Author
-
DeSantis G, Hogan-Schlientz J, Liska G, Kipp S, Sallee R, Wurster M, Kupfer K, and Ansell J
- Subjects
- Adolescent, Adult, Age Factors, Aged, Aged, 80 and over, Cohort Studies, Female, Humans, Male, Middle Aged, Point-of-Care Systems, Retrospective Studies, United States, Young Adult, Anticoagulants blood, Drug Monitoring, International Normalized Ratio, Self Care, Warfarin blood
- Abstract
Objectives: Point-of-care, home international normalized ratio (INR) monitoring (patient self-testing, or PST) provides an opportunity to optimize warfarin therapy as demonstrated in randomized trials. This study sought to determine the quality of warfarin therapy as determined by time in therapeutic INR range (TTR) in patients who perform home monitoring outside of a clinical trial setting., Study Design: Retrospective analysis., Methods: The data base of an independent diagnostic testing facility was retrospectively queried over a 2.5-year period (January 2008-June 2011) and patient TTR was analyzed based on frequency of testing, age, gender, indication for therapy, duration of therapy, and critical value occurrence., Results: A total of 29,457 patients with multiple indications for warfarin therapy comprised the database. The mean TTR for the entire group was 69.7%, with weekly testers achieving a TTR of 74% versus 68.9% for variable testers (testing every 2-4 weeks)(P <.0001). In all categories analyzed (age, indication for anticoagulation, and referral site volume), weekly testers performed significantly better than variable testers. Older individuals had a higher TTR than younger patients. Weekly testers experienced significantly fewer critical values (INR <1.5 or >5.0) than did variable testers., Conclusions: Point-of-care patient self-testing at home achieves high-quality warfarin therapy outside of clinical trials and compares favorably with the results achieved in randomized trials or in anticoagulation clinic settings.
- Published
- 2014
44. [Proximal tibial fractures sustained during alpine skiing - incidence and risk factors].
- Author
-
Pätzold R, Spiegl U, Wurster M, Augat P, Gutsfeld P, Gonschorek O, and Bühren V
- Subjects
- Adolescent, Adult, Age Distribution, Aged, Child, Female, Germany epidemiology, Humans, Incidence, Male, Middle Aged, Risk Factors, Sex Distribution, Young Adult, Athletic Injuries epidemiology, Athletic Performance statistics & numerical data, Knee Injuries epidemiology, Skiing injuries, Snow, Tibial Fractures epidemiology
- Abstract
Background: Prior to introduction of carving skis, complex fractures of the proximal tibia were rarely seen. Recently these fractures are being seen more frequently in connection with alpine skiing. The aim of this study was to find out the incidence of proximal tibia fractures in alpine skiing and to identify possible risk factors., Methods: All patients with proximal tibia fractures related to alpine skiing in a large German ski resort were included. Fracture type, patient and skiing related factors were recorded. Incidence of fractures was determined by using the number of all registered skiers. Multinomial logistic regression analysis was used to calculate the odds ratios for risk factors., Results: Between 2007 and 2010 a total of 188 patients was treated for proximal tibia fractures caused by alpine skiing. Forty-three patients had a type-A injury, 96 patients a type-B injury, and 49 patients a type-C injury. The incidence of injury increased continuously, starting from 2.7 and climbing to 7.0 per 10⁵ skiing days. The risk factors compared to patients with type-A fractures, type-C fracture occurred in older (OR 0.93; 0.89 - 0.97) and heavier (OR 0.86; 0.74 - 0.99) individuals and were more likely on icy snow conditions (OR 0.22; 0.05 - 0.96), higher speed (OR 0.29; 0.09 - 0.97) and skiing skill (OR 0.35; 0.13 - 0.95). These was also seen in artificial and icy snow conditions (OR 0.25; 0.07 - 0.87) when compared to type-B fractures., Conclusion: The incidence of proximal tibia fractures related to skiing has increased over the past four years. Risk factors such as age, BMI, snow conditions, speed, and the skill of the skiers, were identified as causes contributing to complex fractures., (© Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2013
- Full Text
- View/download PDF
45. Triterpene glycosides from the leaves of Pittosporum angustifolium.
- Author
-
Bäcker C, Jenett-Siems K, Siems K, Wurster M, Bodtke A, Chamseddin C, Crüsemann M, and Lindequist U
- Subjects
- Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic therapeutic use, Carcinoma drug therapy, Glycosides chemistry, Glycosides pharmacology, Glycosides therapeutic use, Humans, Inhibitory Concentration 50, Molecular Structure, Oleanolic Acid chemistry, Phytotherapy, Plant Extracts pharmacology, Plant Extracts therapeutic use, Triterpenes chemistry, Triterpenes pharmacology, Triterpenes therapeutic use, Urinary Bladder Neoplasms drug therapy, Glycosides isolation & purification, Plant Extracts chemistry, Plant Leaves chemistry, Rosales chemistry, Triterpenes isolation & purification
- Abstract
Phytochemical investigation of the leaves of Pittosporum angustifolium resulted in the isolation and structural elucidation of nine new triterpene saponins, named pittangretosides A-I (1-9), together with a known compound (10). Mainly by NMR and HRESIMS experiments, eight compounds were identified as A1-barrigenol glycosides (1-7, 10), whereas two compounds exhibited an unusual 17,22-seco-backbone of oleanolic acid (8, 9). All compounds were evaluated for their in vitro cytotoxicities against human urinary bladder carcinoma cells (5637). Only compounds with an angeloyl-residue at C-22 of the aglycone (1-4 and 10) showed antiproliferative effects with IC50 values of 4.1, 5.2, 2.1, 17.9, and 2.4 µM, respectively., (Georg Thieme Verlag KG Stuttgart · New York.)
- Published
- 2013
- Full Text
- View/download PDF
46. A phase 1/2, dose-escalation trial of deferasirox for the treatment of iron overload in HFE-related hereditary hemochromatosis.
- Author
-
Phatak P, Brissot P, Wurster M, Adams PC, Bonkovsky HL, Gross J, Malfertheiner P, McLaren GD, Niederau C, Piperno A, Powell LW, Russo MW, Stoelzel U, Stremmel W, Griffel L, Lynch N, Zhang Y, and Pietrangelo A
- Subjects
- Adult, Aged, Amino Acid Substitution, Benzoates adverse effects, Creatinine blood, Deferasirox, Dose-Response Relationship, Drug, Female, Ferritins blood, Ferritins genetics, Hemochromatosis blood, Hemochromatosis genetics, Hemochromatosis therapy, Homozygote, Humans, Iron Chelating Agents adverse effects, Iron Overload blood, Iron Overload therapy, Male, Middle Aged, Phlebotomy methods, Safety, Transferrin metabolism, Triazoles adverse effects, Benzoates therapeutic use, Hemochromatosis complications, Iron Chelating Agents therapeutic use, Iron Overload drug therapy, Iron Overload etiology, Triazoles therapeutic use
- Abstract
Unlabelled: Hereditary hemochromatosis (HH) is characterized by increased intestinal iron absorption that may result in iron overload. Although phlebotomy is widely practiced, it is poorly tolerated or contraindicated in patients with anemias, severe heart disease, or poor venous access, and compliance can vary. The once-daily, oral iron chelator, deferasirox (Exjade) may provide an alternative treatment option. Patients with HH carrying the HFE gene who were homozygous for the Cys282Tyr mutation, serum ferritin levels of 300-2000 ng/mL, transferrin saturation ≥ 45%, and no known history of cirrhosis were enrolled in this dose-escalation study to characterize the safety and efficacy of deferasirox, comprising a core and an extension phase (each 24 weeks). Forty-nine patients were enrolled and received starting deferasirox doses of 5 (n = 11), 10 (n = 15), or 15 (n = 23) mg/kg/day. Adverse events were generally dose-dependent, the most common being diarrhea, headache, and nausea (n = 18, n = 10, and n = 8 in the core and n = 1, n = 1, and n = 0 in the extension, respectively). More patients in the 15 mg/kg/day than in the 5 or 10 mg/kg/day cohorts experienced increases in alanine aminotransferase and serum creatinine levels during the 48-week treatment period; six patients had alanine aminotransferase > 3 × baseline and greater than the upper limit of normal range, and eight patients had serum creatinine > 33% above baseline and greater than upper limit of normal on two consecutive occasions. After receiving deferasirox for 48 weeks, median serum ferritin levels decreased by 63.5%, 74.8%, and 74.1% in the 5, 10, and 15 mg/kg/day cohorts, respectively. In all cohorts, median serum ferritin decreased to < 250 ng/mL., Conclusion: Deferasirox doses of 5, 10, and 15 mg/kg/day can reduce iron burden in patients with HH. Based on the safety and efficacy results, starting deferasirox at 10 mg/kg/day appears to be most appropriate for further study in this patient population.
- Published
- 2010
- Full Text
- View/download PDF
47. Evaluation of ERalpha, PR and ERbeta isoforms in neoadjuvant treated breast cancer.
- Author
-
Wurster M, Ruoff A, Meisner C, Seeger H, Vogel U, Juhasz-Böss I, Solomayer E, Wallwiener D, Fehm T, and Neubauer H
- Subjects
- Anthracyclines administration & dosage, Biopsy, Breast Neoplasms metabolism, Breast Neoplasms surgery, Cell Proliferation, Chemotherapy, Adjuvant, Female, Germany, Humans, Immunohistochemistry, Neoadjuvant Therapy, Predictive Value of Tests, Taxoids administration & dosage, Time Factors, Tissue Array Analysis, Treatment Outcome, Antineoplastic Agents, Hormonal therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Aromatase Inhibitors therapeutic use, Biomarkers, Tumor metabolism, Breast Neoplasms drug therapy, Estrogen Receptor alpha metabolism, Estrogen Receptor beta metabolism, Receptors, Progesterone metabolism
- Abstract
The actual predictive value of oestrogen receptor (ER) beta for treatment decisions in breast cancer is still unclear. Retrospective studies using preoperative systemic therapy (PST) revealed that chemotherapy but also endocrine therapy can lead to alterations in expression levels of ERalpha and progesterone receptor (PR). The main purpose of this study was to compare ERbeta expression levels before and after neoadjuvant chemotherapy or endocrine therapy and to explore a possible predictive value of ERbeta. Matching 'baseline' biopsies and post-therapy surgical specimens of 69 breast cancer patients treated with neoadjuvant anthracycline- or taxane-based chemotherapy or with aromatase inhibitors were analyzed for expression levels of ERalpha, PR, total ERbeta (ERbetat), ERbeta1, ERbeta2 and the proliferation-related antigen Ki-67 using immunohistochemistry. A marked expression of ERbetat significantly correlates with low proliferation rates after PST (p=0.0013) and with response to it. Further most tumours decreased ERbeta1 expression with PST. A marked ERbeta2 expression was observed predominantly in responders and significantly decreased during chemotherapy (p=0.047). Results on ERalpha and PR corroborate findings of previous studies. Our data demonstrate that changes of ERbeta expression occur during PST and that total ERbeta expression and ERbeta2 may have a predictive value for PST.
- Published
- 2010
48. Synthesis and antimicrobial activity of 4-hydroxy-4-(pyridyl)alk-3-en-2-ones.
- Author
-
Riahi A, Wurster M, Lalk M, Lindequist U, and Langer P
- Subjects
- Bacillus subtilis growth & development, Escherichia coli growth & development, Ketones chemical synthesis, Ketones chemistry, Pyridines chemistry, Staphylococcus aureus growth & development, Anti-Bacterial Agents chemical synthesis, Anti-Bacterial Agents pharmacology, Bacillus subtilis drug effects, Escherichia coli drug effects, Ketones pharmacology, Pyridines chemical synthesis, Pyridines pharmacology, Staphylococcus aureus drug effects
- Abstract
4-Hydroxy-4-(pyridyl)alk-3-en-2-ones were prepared by base-mediated condensation of ketones with pyridinecarboxylates. Several derivatives show weak antimicrobial activity against Gram-positive and Gram-negative bacteria.
- Published
- 2009
- Full Text
- View/download PDF
49. Novel beta-lactam antibiotics synthesized by amination of catechols using fungal laccase.
- Author
-
Mikolasch A, Wurster M, Lalk M, Witt S, Seefeldt S, Hammer E, Schauer F, Jülich WD, and Lindequist U
- Subjects
- Amination, Anti-Bacterial Agents pharmacology, Catalysis, Fungi enzymology, Structure-Activity Relationship, beta-Lactams pharmacology, Anti-Bacterial Agents chemical synthesis, Catechols chemistry, Laccase metabolism, beta-Lactams chemical synthesis
- Abstract
Novel cephalosporins, penicillins, and carbacephems were synthesized by amination of catechols with amino-beta-lactams like cefadroxil, amoxicillin, ampicillin and the structurally related carbacephem loracarbef using laccase from Trametes sp. All isolated monoaminated products inhibited the growth of several Gram positive bacterial strains in the agar diffusion assay, among them methicillin-resistant Staphylococcus aureus strains and vancomycin-resistant Enterococci. Observed differences in the cytotoxicity and in vivo activity in a "Staphylococcus-infected, immune suppressed mouse" model are discussed.
- Published
- 2008
- Full Text
- View/download PDF
50. Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from Yemen.
- Author
-
Al-Fatimi M, Wurster M, Schröder G, and Lindequist U
- Subjects
- Anti-Bacterial Agents administration & dosage, Antifungal Agents administration & dosage, Antineoplastic Agents, Phytogenic administration & dosage, Antioxidants administration & dosage, Biphenyl Compounds, Cell Line, Drug Screening Assays, Antitumor, Free Radicals metabolism, Fungi drug effects, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Medicine, Traditional, Microbial Sensitivity Tests, Picrates, Plants, Medicinal, Yemen, Anti-Bacterial Agents pharmacology, Antifungal Agents pharmacology, Antineoplastic Agents, Phytogenic pharmacology, Antioxidants pharmacology, Phytotherapy, Plant Extracts pharmacology
- Abstract
Ninety crude extracts, including dichloromethane, methanol and aqueous extracts from 30 medicinal plants used in the Yemeni ethnomedicine to treat common infections, were screened in vitro for antimicrobial activities against three Gram-positive bacteria and two Gram-negative bacteria, Candida maltosa and five opportunistic human fungal pathogens (two yeasts, three hyphomycetes). Most of the plants showed antibacterial activities. Extracts from Tamarindus indica flowers and Ficus vasta fruits have been the most active. Of the 30 plants tested, 13 showed antifungal activity (40%) against one ore more human pathogenic fungi. The strongest inhibition was exhibited by Azima tetracantha (fruits), Sansevieria ehrenbergii (fruits) and Solanum incanum (fruits). Ten methanol extracts, especially those of Acacia asak barks and Solanum nigrum fruits, showed effective free radical scavenging activities in the DPPH assay. Remarkable cytotoxic activity against FL-cells was shown only for five plants, among them Plicosepalus curviflorus (stems).
- Published
- 2007
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.