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32 results on '"Wu, Yuanzi"'

11. Preliminary Study on Hepatoprotective Effect and Mechanism of (-)-Epigallocatechin-3-gallate against Acetaminophen-induced Liver Injury in Rats

Author

Lin, Yongxu, Huang, Juan, Gao, Tingfang, Wu, Yuanzi, Huang, Da, Yan, Fen, and Weng, Zuquan

Subjects
digestive, oral, and skin physiology, APAP, food and beverages, heterocyclic compounds, Original Article, ROS, sense organs, Liver Injury, complex mixtures, EGCG, Mitochondria
Abstract

Antipyretic acetaminophen (APAP) is a commonly used drug that generally associates with liver injury. (-)-Epigallocatechin-3-gallate (EGCG), an active polyphenol extracted from green tea, is extensively reported to have the potential to impact a variety of human diseases. However, few studies were reported regarding the protective effect of EGCG on APAP-induced liver injury and the mechanism is still unclear. In this study, in-vitro and in-vivo experiments were carried out to verify the hepatoprotective effect of EGCG against APAP-induced liver injury and explore the potential mechanism. Results indicated that EGCG effectively relieved the liver injury caused by APAP, as well as APAP-induced mitochondrial dysfunction. The protective role of EGCG was not only attributed to its antioxidant capacity; but also might be related to the protective effect on hepatic mitochondrial impairment; based on that, EGCG could improve the membrane potential and activities of the respiratory chain complexes in liver mitochondria. Our study casts a new light on the mechanism of EGCG’s hepatoprotective effect and suggests that EGCG has considerable potential in developing tonics for relieving APAP-induced liver injury.

Published
2021

14. Integrated poly(dimethysiloxane) with an intrinsic nonfouling property approaching 'absolute' zero background in immunoassays

Author

Ma, Hongwei, Wu, Yuanzi, Yang, Xiaoli, Liu, Xing, He, Jianan, Fu, Long, Wang, Jie, Xu, Hongke, Shi, Yi, and Zhong, Renqian

Subjects
Enzyme-linked immunosorbent assay -- Methods, Siloxanes -- Chemical properties, Siloxanes -- Composition, Proteins -- Chemical properties, Tumors -- Chemical properties, Biological markers -- Chemical properties, Chemistry
Abstract

The key to achieve a highly sensitive and specific protein microarray assay is to prevent nonspecific protein adsorption to an 'absolute' zero level because any signal amplification method will simultaneously amplify signal and noise. Here, we develop a novel solid supporting material, namely, polymer coated initiator integrated poly(dimethysiloxane) (iPDMS), which was able to achieve such 'absolute' zero (i.e., below the detection limit of instrument). The implementation of this iPDMS enables practical and high-quality multiplexed enzyme-linked immunoserbent assay (ELISA) of 11 tumor markers. This iPDMS does not need any blocking steps and only require mild washing conditions. It also uses on an average 8-fold less capture antibodies compared with the mainstream nitrocellulose (NC) film. Besides saving time and materials, iPDMS achieved a limit-of-detection (LOD) as low as 19 pg [mL.sup.-1], which is sufficiently low for most current clinical diagnostic applications. We expect to see an immediate impact of this iPDMS on the realization of the great potential of protein microarray in research and practical uses such as large scale and high-throughput screening, clinical diagnosis, inspection, and quarantine. 10.1021/ac101277e

Published
2010

32. Polyphenol extract from superheated steam processed tea waste attenuates the oxidative damage in vivo and in vitro.

Author

Gao, Tingfang, Shi, Yuhong, Xue, Ying, Yan, Fen, Huang, Da, Wu, Yuanzi, and Weng, Zuquan

Subjects
SUPERHEATED steam, REACTIVE oxygen species, LIVER mitochondria, FOOD additives, POLYPHENOL oxidase, TEA, BREWING
Abstract

In this study, tea polyphenols (TPs) was first extracted from tea waste by superheated steam (SS) pretreated ultrasonic‐assisted hydrothermal extraction (UAH). The optimized strategy presented extracts with the extraction yield up to 21.19% with a significantly higher antioxidant ability, compared with the one without SS pretreatment. Further investigation proved that the SS suppressed the polyphenol oxidase activity of the TPs extract. The ability to scavenge the free radicals were compared in mouse liver mitochondria. Mitochondrial swelling, mitochondrial membrane potential (MMP), cardiolipin peroxidation, and respiratory chain complex (RCC) I–V activities were also evaluated as the index of the mitochondrial oxidative damage. The study supports evidence that the TPs extract exhibited significant protection against oxidative damage on mitochondrial. Furthermore, the effect of TPs on antioxidant ability in zebrafish embryo was evaluated. After TPs pretreatment for 1 day, zebrafish embryos showed a significantly higher survival rate as well as heart rate when facing the oxidative stress. Practical applications: Polyphenols from tea leaves have been viewed as an antioxidant additive in food, mainly due to the ability of scavenging free radicals and reactive oxygen species. The results of this study suggest that the SS pretreatment could be used as an efficient method to extract TPs from the tea waste for the prevention of oxidative damage in the mouse liver mitochondria and zebrafish embryos. [ABSTRACT FROM AUTHOR]

Published
2020
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