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Integrated poly(dimethysiloxane) with an intrinsic nonfouling property approaching 'absolute' zero background in immunoassays

Integrated poly(dimethysiloxane) with an intrinsic nonfouling property approaching 'absolute' zero background in immunoassays

Authors :
Ma, Hongwei
Wu, Yuanzi
Yang, Xiaoli
Liu, Xing
He, Jianan
Fu, Long
Wang, Jie
Xu, Hongke
Shi, Yi
Zhong, Renqian
Source :
Analytical Chemistry. August 1, 2010, Vol. 82 Issue 15, p6338, 5 p.
Publication Year :
2010

Abstract

The key to achieve a highly sensitive and specific protein microarray assay is to prevent nonspecific protein adsorption to an 'absolute' zero level because any signal amplification method will simultaneously amplify signal and noise. Here, we develop a novel solid supporting material, namely, polymer coated initiator integrated poly(dimethysiloxane) (iPDMS), which was able to achieve such 'absolute' zero (i.e., below the detection limit of instrument). The implementation of this iPDMS enables practical and high-quality multiplexed enzyme-linked immunoserbent assay (ELISA) of 11 tumor markers. This iPDMS does not need any blocking steps and only require mild washing conditions. It also uses on an average 8-fold less capture antibodies compared with the mainstream nitrocellulose (NC) film. Besides saving time and materials, iPDMS achieved a limit-of-detection (LOD) as low as 19 pg [mL.sup.-1], which is sufficiently low for most current clinical diagnostic applications. We expect to see an immediate impact of this iPDMS on the realization of the great potential of protein microarray in research and practical uses such as large scale and high-throughput screening, clinical diagnosis, inspection, and quarantine. 10.1021/ac101277e

Details

Language :
English
ISSN :
00032700
Volume :
82
Issue :
15
Database :
Gale General OneFile
Journal :
Analytical Chemistry
Publication Type :
Academic Journal
Accession number :
edsgcl.234569245