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Key message We identified marker-trait associations for key faba bean agronomic traits and genomic signatures of selection within a global germplasm collection. Abstract Faba bean (Vicia faba L.) is a high-protein grain legume crop with great potential for sustainable protein production. However, little is known about the genetics underlying trait diversity. In this study, we used 21,345 high-quality SNP markers to genetically characterize 2678 faba bean genotypes. We performed genome-wide association studies of key agronomic traits using a seven-parent-MAGIC population and detected 238 significant marker-trait associations linked to 12 traits of agronomic importance. Sixty-five of these were stable across multiple environments. Using a non-redundant diversity panel of 685 accessions from 52 countries, we identified three subpopulations differentiated by geographical origin and 33 genomic regions subjected to strong diversifying selection between subpopulations. We found that SNP markers associated with the differentiation of northern and southern accessions explained a significant proportion of agronomic trait variance in the seven-parent-MAGIC population, suggesting that some of these traits were targets of selection during breeding. Our findings point to genomic regions associated with important agronomic traits and selection, facilitating faba bean genomics-based breeding.

Faba bean (Vicia faba L.) is a high-protein grain legume crop with great potential for further cultivation. However, little is known about the genetics underlying trait diversity. In this study, we use 21,345 high-quality SNP markers to genetically characterise 2,678 faba bean genotypes. We perform genome-wide association studies of key agronomic traits using a Seven-parent-MAGIC population and detect 238 significant marker-trait associations linked to 12 traits of agronomic importance, with 65 of these being stable across multiple environments. Using a non-redundant diversity panel of 685 accessions from 52 countries, we identify 3 subpopulations differentiated by geographical origin and 33 genomic regions subject to strong diversifying selection between subpopulations. We find that SNP markers associated with the differentiation of northern and southern accessions were able to explain a significant proportion of agronomic trait variance in the Seven-parent-MAGIC population, suggesting that some of these traits have played an important role in breeding. Altogether, our findings point to genomic regions associated with important agronomic traits and selection in faba bean, which can be used for breeding purposes.Key MessageWe identified marker-trait associations for key faba bean agronomic traits and genomic signatures of selection within a global germplasm collection.

Fibre optic sensors are excellent tools to use for monitoring high-voltage current collectors. Because of their small cross section and electrical neutrality, they are easily integrated into the current collector strip and are well specialized for detection of high-speed load events. The conventional contact force measurement with four force sensors below the collector strips can also be simplified by using fibre optic force and acceleration sensors.

Optical fiber sensors find increasing applications in areas ranging from structural health monitoring to biophotonic sensing. The unique properties of optical fibers and sensor structures support such progress: complete immunity to electromagnetic fields, high voltage, lightning; operation in explosive or chemically aggressive and corrosive media (energy, oil, gas); light weight, miniaturized, flexible; low thermal conductivity, temperature-resistant material (highend and low-end temperatures); low-loss, noninterfering signal transmission, ability to operate over long distances (remote sensing), multiplexing capability (sensor networks), and structure monitoring by embedding in composite materials (smart structures). New optical wave-guide materials and systems like plastic optical fibers, sapphire optical fiber, photonic crystal fibers, and other nanostructures stimulate corresponding new sensor developments making use of the specific material advantages. In this issue you will find some examples of actual research, scientific technological development, and application of optical fiber sensors: a review on the status of photonic crystal fibers for sensing applications; fiber Bragg grating resonator structures resulting in an all-fiber DBRbased sensor interrogation system for measuring acoustic waves; the application of plastic optical fibers for sensing of fuel leakage in soil as well as of alcohol concentration in liquors; application of laser Doppler velocimetry for measurement of local blood velocity profiles.

Cruciferin (cru) and napin (nap) were negatively correlated and the cru/nap ratio was closely negative correlated with glucosinolate content indicating a link between the two biosynthetic pathways. Canola-type oilseed rape (Brassica napus L.) is an economically important oilseed crop in temperate zones. Apart from the oil, the canola protein shows potential as a value-added food and nutraceutical ingredient. The two major storage protein groups occurring in oilseed rape are the 2 S napins and 12 S cruciferins. The aim of the present study was to analyse the genetic variation and the inheritance of napin and cruciferin content of the seed protein in the winter oilseed rape doubled haploid population Express 617 × R53 and to determine correlations to other seed traits. Seed samples were obtained from field experiments performed in 2 years at two locations with two replicates in Germany. A previously developed molecular marker map of the DH population was used to map quantitative trait loci (QTL) of the relevant traits. The results indicated highly significant effects of the year and the genotype on napin and cruciferin content as well as on the ratio of cruciferin to napin. Heritabilities were comparatively high with 0.79 for napin and 0.77 for cruciferin. Napin and cruciferin showed a significant negative correlation (−0.36**) and a close negative correlation of the cru/nap ratio to glucosinolate content was observed (−0.81**). Three QTL for napin and two QTL for cruciferin were detected, together explaining 47 and 35 % of the phenotypic variance. A major QTL for glucosinolate content was detected on linkage group N19 whose confidence interval overlapped with QTL for napin and cruciferin content. Results indicate a relationship between seed protein composition and glucosinolate content.

Following winter oilseed rape cultivation, considerable numbers of volunteer oilseed rape plants may occur in subsequent years in following crops. The appearance of volunteer oilseed rape plants is based on the capability of the seeds to become secondary dormant and to survive in this stage for many years in the soil. Genetic reduction of secondary seed dormancy in oilseed rape could provide a means to reduce the frequency of volunteer plants and especially the dispersal of transgenic oilseed rape. The objective of the present study was to analyse the inheritance of primary and secondary seed dormancy in a winter oilseed rape doubled haploid population derived from the cross Express 617 × R53 and to study correlations to other seed traits. Field experiments were performed in Germany for 2 years at two locations with two replicates. Seeds harvested from open pollinated plants were used for all analyses, including a laboratory test for seed dormancy. A previously developed molecular marker map of the doubled haploid population was used to map QTL of the relevant traits. For primary, secondary and total seed dormancy, the results showed significant effects of the genotypes and their interactions, with years and locations. Two, four and five QTL were detected for primary, secondary and total seed dormancy which explained 19, 35 and 42 % of the phenotypic variance, respectively. Results show that secondary seed dormancy is a heritable trait and that selection for low secondary seed dormancy is possible.

In the EU railway network, a multitude of railway companies runs their rolling stock on tracks which are not in their own responsibility. Consequently, permanent monitoring of security-relevant parameters becomes an issue of increasing importance. In order to characterize the status of the catenary–current collector interface, compliance with the specified contact force between them is a generally agreed-upon quality parameter. We report here on an approach to monitor contact forces (without correction of dynamic parts in this first step) inline using a potentially low-cost fiber optic sensing system which is insensitive to the high electrical potential and to field changes. It was implemented and tested successfully on regular trains by replacing the current collector with a sensor-embedded one, and by adding a small-sized signal processing unit in the driver cabin. In this article, we describe the construction and application results of the system and discuss its advantages and limitations. Sensor characteristics are investigated with the help of a mechanical model. The system has shown its potential during test drives on railway tracks in Switzerland.

Microspore culture has become an important tool in many species, includingBrassicas, for the production of entirely homozygous lines, so called double haploid (DH) lines. The primary products of microspore culture are embryo-like structures, called microspore-derived embryos (MDEs). A major problem in the development of DH lines is the often low efficiency of Direct Embryo to Plant Conversion (DEPC). During the development of DH populations, favourable alleles of genes affecting the DEPC rate will be under selection. This selection should lead to skewed segregations at markers linked to these genes. By comparing skewed marker segregations in four populations, a population of doubled haploid plantlets, a haploid and a doubled haploid MDE population, and a BC1population, 20 genomic regions were identified, which showed patterns of skewed segregations across the populations, indicative of the segregation of genetic factors controlling DEPC rates. Four regions and eight intervarietal substitution lines (ISLs) with donor segments overlapping these regions were selected for further studies. Three ISLs, ER654, ER661 and ER653 with DEPC rates of 49.1, 54.5 and 57.2 %, showed significantly reduced DEPC rates compared to the rate of the recurrent parent of 76.5 %. By comparing donor segments between the significant and the non-significant lines, eight genomic regions were identified that may contain genetic factors controlling the DEPC rate in rapeseed. These regions range in size from 0 (represented by just one marker) to 16.5 cM and cover together just 1.33 % of the genetic map used to characterize the donor segments in the ISLs.

The inherent temperature dependence of optical fibre Bragg gratings is caused mainly by the positive thermo-optic effect of the fibre core material, and it results in an increase of Bragg wavelength with temperature. Special mounting techniques may be designed in order to compensate this thermo-optic effect by counter-acting effects: decreasing mechanical strain, as well as a decreasing effective refractive index for the guided light wave by evanescent field interaction with a liquid of negative thermo-optic effect. Because of the non-linear interaction characteristics, exact temperature compensation is obtained for a certain temperature, which depends on the design parameters. Such stabilised fibre Bragg gratings find application as wavelength references in Bragg grating sensor networks.

QTL mapping by association analysis has recently gained interest in plant breeding research as an alternative to QTL mapping in segregating populations from biparental crosses. In a first experiment on whole-genome association analysis in rapeseed, 684 mapped AFLP markers were tested for association with 14 traits in a set of 84 canola quality winter rapeseed cultivars. For association analysis a general linear model was used. By testing significance of marker–trait associations against a false discovery rate of 0.2, between 1 and 34 associated markers were found for 10 of the 14 traits. Taking into account linkage disequilibrium between the significant markers, these markers represent between 1 and 22 putative QTL for the respective traits. The minimum phenotypic variance explained by the QTL for the different traits ranged from 15% to 53%. A subset of 27 markers were significantly associated with two or more traits. These markers were predominantly shared between traits that were significantly correlated at the phenotypic level. The results show clearly that in rapeseed, QTL mapping by association analysis is a viable alternative to QTL mapping in segregating populations.

Hollow glass micro-spheres, firstly used to make fiber optic sensors for high hydrostatic pressure, have been interrogated using a high-resolution CCD-based spectrometer, to give far better precision than conventional spectrometric read out. It is found that these simple, low-cost micro-sensors have excellent sensitivity to both static and dynamic pressure, and have the advantage of being hermetically sealed. Many other application areas are foreseen for these low-cost sensors.

Fiber Bragg grating (FBG) sensors in single-mode optical fibers are widely applied for measurement of temperature and strain. If exposing FBG sensors to an external analyte by planar side-polishing technique of the fiber, evanescent-field interaction yields a Bragg wavelength shift also by changing the refractive index of the analyte. Deposition of sensor-specific transducer layers on the side-polished fiber can specify this spectrally encoding and network-capable optochemical fiber Bragg grating refractometry to the monitoring of specific substances, absorbed gases and vapors. In this paper, the sensor principle is demonstrated for the example of a hydrogen gas sensor based on a palladium thin-film transducer. Hydrogen in 0.1–4% volume concentration range can be monitored by the spectral shift of the Bragg wavelength, which is caused by the decreasing complex refractive index of Pd with increasing absorption of hydrogen.

While conventional electrical resistance strain gages show increasing cross-sensitivities to temperature and magnetic field with decreasing temperature down to liquid helium, it has been found that fiber-optic Bragg grating strain sensors show negligible thermo-optic and magneto-optic effects in cryogenic environments; therefore, they allow reliable strain measurements. These specific application advantages of optical fiber Bragg grating sensors at low temperatures, together with the electrical isolation and low electro-magnetic interference, low thermal conductivity and their multiplexing capability, make them attractive for structural health monitoring in cryogenic devices such as superconductive magnets. In this paper we present low temperature characteristics of fiber Bragg grating-based sensors and address application-based side effects such as induced birefringence.

We report about fiber Bragg gratings (FBGs) inscribed in two different types of small-core Ge-doped photonic crystal fibers with a UV laser. Sensing properties of the FBGs were systematically investigated by means of demonstrating the responses of Bragg wavelengths to temperature, strain, bending, and transverse-loading. The Bragg wavelength of the FBGs shifts toward longer wavelengths with increasing temperature, tensile strain, and transverse-loading. Moreover, the bending and transverse-loading properties of the FBGs are sensitive to the fiber orientations. The reasonable analyses for these sensing properties also are presented.

Increasing oil content and improving the fatty acid composition in the seed oil are important breeding goals for rapeseed (Brassica napus L.). The objective of the study was to investigate a possible relationship between fatty acid composition and oil content in an oilseed rape doubled haploid (DH) population. The DH population was derived from a cross between the German cultivar Sollux and the Chinese cultivar Gaoyou, both having a high erucic acid and a very high oil content. In total, 282 DH lines were evaluated in replicated field experiments in four environments, two each in Germany and in China. Fatty acid composition of the seed oil was analyzed by gas liquid chromatography and oil content was determined by NIRS. Quantitative trait loci (QTL) for fatty acid contents were mapped and their additive main effects were determined by a mixed model approach using the program QTLMapper. For all fatty acids large and highly significant genetic variations among the genotypes were observed. High heritabilities were determined for oil content and for all fatty acids (h 2 = 0.82 to 0.94), except for stearic acid content (h 2= 0.38). Significant correlations were found between the contents of all individual fatty acids and oil content. Closest genetic correlations were found between oil content and the sum of polyunsaturated fatty acids (18:2 + 18:3; r G = −0.46), the sum of monounsaturated fatty acids (18:1 + 20:1 + 22:1; r G = 0.46) and palmitic acid (16:0; r G = −0.34), respectively. Between one and eight QTL for the contents of the different fatty acids were detected. Together, their additive main effects explained between 28% and 65% of the genetic variance for the individual fatty acids. Ten QTL for fatty acid contents mapped within a distance of 0 to 10 cM to QTL for oil content, which were previously identified in this DH population. QTL mapped within this distance to each other are likely to be identical. The results indicate a close interrelationship between fatty acid composition and oil content, which should be considered when breeding for increased oil content or improved oil composition in rapeseed.

Oil content in rapeseed (Brassica napus L.) is generally regarded as a character with high heritability that is negatively correlated with protein content and influenced by plant developmental and yield related traits. To evaluate possible genetic interrelationships between these traits and oil content, QTL for oil content were mapped using data on oil content and on oil content conditioned on the putatively interrelated traits. Phenotypic data were evaluated in a segregating doubled haploid population of 282 lines derived from the F(1) of a cross between the old German cultivar Sollux and the Chinese cultivar Gaoyou. The material was tested at four locations, two each in Germany and in China. QTLMapper version 1.0 was used for mapping unconditional and conditional QTL with additive (a) and locus pairs with additive x additive epistatic (aa) effects. Clear evidence was found for a strong genetic relationship between oil and protein content. Six QTL and nine epistatic locus pairs were found, which had pleiotropic effects on both traits. Nevertheless, two QTL were also identified, which control oil content independent from protein content and which could be used in practical breeding programs to increase oil content without affecting seed protein content. In addition, six additional QTL with small effects were only identified in the conditional mapping. Some evidence was apparent for a genetic interrelationship between oil content and the number of seeds per silique but no evidence was found for a genetic relationship between oil content and flowering time, grain filling period or single seed weight. The results indicate that for closely correlated traits conditional QTL mapping can be used to dissect the genetic interrelationship between two traits at the level of individual QTL. Furthermore, conditional QTL mapping can reveal additional QTL with small effects that are undetectable in unconditional mapping.

A fibre Bragg grating sensor system has been installed and successfully operated in a horizontal-axis wind turbine since February 2004. We herewith report the requirements, design and construction parameters of the sensor system for continuous on-line monitoring of bending loads of the rotor blades and provide examples of the monitoring results.

Tocopherols are natural antioxidants in vegetable oils and are important dietary nutrients. Enhanced tocopherol content has become an important objective in oilseed rape breeding. A segregating DH population was tested for 2 years at two locations in replicated field trials. Genotypic differences occurred for alpha-, gamma- and total tocopherol content as well as alpha/gamma-tocopherol ratio, but highly significant genotype x environment interactions resulted in low heritabilities. Using a mixed-model composite interval mapping approach between one and five QTL with additive and/or additive x environment interaction effects could be mapped for alpha-, gamma- and total tocopherol content and alpha/gamma-tocopherol ratio. In addition, one to six locus pairs with epistatic interaction effects were identified, indicating a strong contribution of epistasis to trait variation. In total, the additive and epistatic effects explained between 28% (alpha-tocopherol content) and 73% (total tocopherol content) of the genotypic variance in the population, with individual QTL and locus pairs contributing between 7.5 and 29.2% of variance. Considering the low heritabilities of the tocopherol traits, the results of this study indicate that marker-assisted selection may be an efficient strategy in a breeding program for enhanced tocopherol content in rapeseed.

An opto-chemical in-fibre Bragg grating (FBG) sensor for refractive index measurement in liquids has been developed using fibre side-polishing technology. At a polished site where the fibre cladding has partly been removed, a FBG is exposed to a liquid analyte via evanescent field interaction of the guided fibre mode. The Bragg wavelength of the FBG is obtained in terms of its dependence on the refractive index of the analyte. Modal and wavelength dependences have been investigated both theoretically and experimentally in order to optimize the structure of the sensor. Using working wavelengths far above the cut-off wavelength results in an enhancement of the sensitivity of the sensor. Measurements with different mode configurations lead to the separation of cross sensitivities. Besides this, a second FBG located in the unpolished part can be used to compensate for temperature effects. Application examples for monitoring fuels of varying quality as well as salt concentrations under deep borehole conditions are presented.

We report a thermo-optic switching effect with a high extinction ratio of 30 dB by means of filling a fluid into air holes of a solid-core photonic crystal fiber (PCF). Such an effect can perform a turn on-off operation of the transmitted light via a small temperature adjustment of ±10°C. The switching function attributes to the absorption of the filled fluid in combination with the interaction between the core mode and the excited ?fluid rod? modes, resulting from the thermo-optic effect of the filled fluid.

Microsatellite markers have assumed great significance in biological research. The isolation and characterisation of microsatellites involves DNA library construction and screening, DNA sequencing, primer design and PCR optimisation. When a microsatellite is situated close to the beginning or end of a cloned fragment, specific primers cannot be designed for one of the flanking sequences, thus hindering the utilisation of such microsatellites as markers. The present approach was to use one 5´-anchored primer complementary to the microsatellite sequence in combination with one specific Cy5- labelled primer with a view to retrieving useful microsatellites, which would otherwise be lost. Six pairs of a 5´ anchored primer and a specific primer were used across a set of 31 Brassica napus winter cultivars and one accession each of five additional Brassica species. Using laser fluorometry a single labelled product was observed after amplification with each of four primer pairs, and one primer pair gave two labelled products. Three products corresponded in size with the products expected if 5´ anchoring was effective, indicating the amplification of locus-specific full-length products including all of the microsatellite repeats. All six primer pairs showed polymorphisms across the Brassica species examined, but only one primer pair showed polymorphisms within B. napus, making it useful for genetic analysis in rapeseed cultivars. The other primer pairs could be useful in studying gene introgression into B. napus or for investigating interspecific crosses involving different Brassica species.

In this letter, the transverse loading characteristics of Bragg gratings in microstructured optical fibers were investigated by use of an active fiber depolarizer. Increasing transverse load shifts the Bragg wavelength to longer wavelengths; its sensitivity to transverse load decreases with increasing volume of air holes around the fiber core. Such transverse loading characteristics were found to be dependent on the fiber orientations.

The objective of the present study was to estimate the abundance and degree of polymorphism of simple sequence repeat (SSR) markers in rapeseed. By screening about 45000 clones of a small inserts library of rapeseed total DNA the abundances of GA/TC and CA/TG simple sequence repeats in the rapeseed genome were estimated to be approximately one repeat every 100 kb and 400 kb, respectively. After sequencing 13 positive clones, primer pairs could be designed for 11 microsatellite loci. Seven of these primer pairs produced reproducible amplification products in a set of 31 rapeseed genotypes, with one pair amplifying two independent products, giving a total of eight amplified loci. The different microsatellite loci displayed between one and three visible alleles. At four loci, additional null alleles were observed. With up to four alleles, polymorphic microsatellite markers show significantly higher allele numbers in rapeseed than restriction fragment length polymorphism (RFLP) markers. Four of the eight microsatellite markers could be mapped on four different linkage groups of an RFLP map of the rapeseed genome.

The operation of a novel device – a waveguide surface plasmon resonance sensor with a UV-written Bragg grating – is theoretically analysed using two methods. In the simple perturbation approach, the metal/dielectric layer system supporting the resonance excitation of the surface plasma wave is considered to be a perturbation of the original dielectric waveguide with Bragg grating that is analysed using a coupled-mode theory. The second approach consists of the rigorous method of bi-directional mode expansion and propagation using the Floquet mode formalism developed recently for the analysis of waveguide grating structures. The results of both approaches are mutually compared, and the operation characteristics of this novel sensing device are briefly described.

The applicability of random amplified polymorphic DNA (RAPD) markers in the cultivated rubber tree, Hevea, was evaluated using 43 decamer oligonucleotide primers in a set of 24 clones selected in different South-East Asian countries. A total of 220 0.35–3.5 kb DNA fragments were amplified, of which 111 were polymorphic. Of these, 80 fragments (RAPD markers) which were repeatable and clearly scorable across all genotypes were used to estimate genetic distances among the clones tested. The estimated genetic distances ranged from 0.05 (RRII 308 and PB 5/51) to 0.75 (RRIC 100 and SCATC 88–13). A mean genetic distance of 0.5 indicates a rather high genetic variability among the tested clones. As expected, because of the breeding history of Hevea, UPGMA cluster analysis and Principal Coordinate Analysis (PCoA) indicated the absence of a distinct geographical grouping. The possible application of RAPD markers for clone identification and also for analysis of genetic relationships among Hevea clones is discussed.

We report a thermo-optic attenuator by means of filling a fluid into air holes of a solid-core photonic crystal fiber. Such an attenuator can perform a 30dB attenuation operation of the transmitted light near 1200 nm via a small temperature adjustment of ±10°C.

A F1 microspore-derived DH population, previously used for the development of a rapeseed RFLP map, was analysed for the distribution of erucic acid and seed oil content. A clear three-class segregation for erucic acid content could be observed and the two erucic acid genes of rapeseed were mapped to two different linkage groups on the RFLP map. Although the parents of the segregating DH population showed no significant difference in seed oil content, in the DH population a transgressive segregation in oil content was observed. The segregation closely followed a normal distribution, characteristic of a quantitative trait. Using the program MAPMAKER/QTL, three QTLs for seed oil content could be mapped on three different linkage groups. The additive effects of these QTLs explain about 51% of the phenotypic variation observed for this trait in the DH population. Two of the QTLs for oil content showed a close association in location to the two erucic acid genes, indicating a direct effect of the erucic acid genes on oil content.

A linkage map of the rapeseed genome comprising 204 RFLP markers, 2 RAPD markers, and 1 phenotypic marker was constructed using a F1 derived doubled haploid population obtained from a cross between the winter rapeseed varieties 'Mansholt's Hamburger Raps' and 'Samourai'. The mapped markers were distributed on 19 linkage groups covering 1441 cM. About 43% of these markers proved to be of dominant nature; 36% of the mapped marker loci were duplicated, and conserved linkage arrangements indicated duplicated regions in the rapeseed genome. Deviation from Mendelian segregation ratios was observed for 27.8% of the markers. Most of these markers were clustered in 7 large blocks on 7 linkage groups, indicating an equal number of effective factors responsible for the skewed segregations. Using cDNA probes for the genes of acyl-carrier-protein (ACP) and β-ketoacyl-ACP-synthase I (KASI) we were able to map three and two loci, respectively, for these genes. The linkage map was used to localize QTLs for seed glucosinolate content by interval mapping. Four QTLs could be mapped on four linkage groups, giving a minimum number of factors involved in the genetic control of this trait. The estimated effects of the mapped QTLs explain about 74% of the difference between both parental lines and about 61.7 % of the phenotypic variance observed in the doubled haploid mapping population.

The raising demand for increase of efficiency and reduction of costs in power generation causes a mind change and promotes the commercial use of fiber optical sensors for health monitoring and control purposes.

A fiber optic Bragg grating (FBG) sensor and interrogation scheme has been designed to capture the momentary peak pressure forces in the nip of two adjacent paper machine rolls. The spatial distribution of these nip forces along circumference and length of the roll, for production speeds of up to 2000 m/min are investigated. Additionally, this FBG sensor system measures the temperature distribution in the roll cover. FBG sensor embedding has been optimized for the implementation of pressure force measurements in various roll cover materials. High strength draw tower grating (DTG) sensor arrays were used for the embedding process combined with spectrometric interrogation and autonomous power supply technologies. This results in an extremely robust fiber optic sensor system for operation at rotation speeds of 700 rpm, equivalent to centrifugal accelerations of 300 G. These measurements enable immediate quality control during various stages of the high-speed paper production process. © (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Special fiber Bragg grating (FBG) sensor embedding and interrogation schemes have been designed to capture the momentary peak pressure forces in the nip of adjacent paper machine rolls, and the spatial distribution of these nip forces along circumference and length of the roll, for production speeds of up to 2000 m/min. Additionally, this FBG sensor system measures the temperature distribution in the roll cover. FBG sensor embedment has been investigated and optimized for the implementation of pressure force measurements in various roll cover materials. These measurements enable immediate quality control during various stages of the production process. Draw Tower Grating sensor arrays, simultaneously performing spectrometric interrogation, and autonomous power supply technologies result in an extremely robust fiberoptic sensor system operating at rotation speeds of 700 rpm, equivalent to centrifugal accelerations of 300 G.

Design and realization of fiber Bragg grating sensor systems are described for implementation of structural health monitoring in energy plants. Application examples in conventional, renewable, and nuclear energies demonstrate their potential in advanced energetics.

We report about fiber Bragg gratings (FBGs) inscribed in two different types of small-core Ge-doped photonic crystal fibers with a UV laser. Sensing applications of the FBGs were systematically investigated by means of demonstrating the responses of Bragg wavelengths to temperature, strain, bending, and transverse-loading. The Bragg wavelength of the FBGs shifts toward longer wavelengths with increasing temperature, tensile strain, and transverse-loading. Moreover, the bending and transverse-loading properties of the FBGs are sensitive to the fiber orientations. The reasonable analyses for these sensing properties also are presented

Optical resonance coupling between a side-polished fiber and thin film waveguides has been investigated in the presence of biochemical adsorbates. The shift of the resonance wavelengths was found to be highly sensitive to the capture of target DNA recognition elements with Au nanoparticle markers, allowing for a sensitivity limit of 10 particles on the side-polished fiber core area (2000 μm²) during on-line measurements using a polychromator spectrometer.

Various spectral-encoded fiber optic sensor concepts and advanced system solutions for application in energy facility monitoring have been investigated. The technological maturity, high performance and reliability of multiplexed fiber Bragg grating (FBG) sensor arrays and networks for the measurement of temperature, dynamic strain, air flow, and magnetic field distributions in electric power generators increasing their efficiency will be demonstrated by selected examples of field testing under harsh environmental conditions. For high-temperature combustion monitoring in gas turbines, beside silica FBGs with enhanced temperature stability also sapphire FBGs and Fabry-Perot sensors have been tested and evaluated as well as fiber-based black-body thermal radiation sensors. Finally, the potential of FBG sensors for application in cryo-energetic facilities such as super-conductive high-power motors and experimental nuclear fusion reactors will be discussed.

QTL mapping by association analysis has recently gained interest in plant breeding research as an alternative to QTL mapping in segregating populations from biparental crosses. In a first experiment on whole-genome association analysis in rapeseed, 684 mapped AFLP markers were tested for association with 14 traits in a set of 84 canola quality winter rapeseed cultivars. For association analysis a general linear model was used. By testing significance of marker-trait associations against a false discovery rate of 0.2, between 1 and 34 associated markers were found for 10 of the 14 traits. Taking into account linkage disequilibrium between the significant markers, these markers represent between 1 and 22 putative QTL for the respective traits. The minimum phenotypic variance explained by the QTL for the different traits ranged from 15% to 53%. A subset of 27 markers were significantly associated with two or more traits. These markers were predominantly shared between traits that were significantly correlated at the phenotypic level. The results show clearly that in rapeseed, QTL mapping by association analysis is a viable alternative to QTL mapping in segregating populations.

Advantages of optical fiber Bragg grating sensors at low temperatures, are electrical isolation, low electro-magnetic interference, low thermal conductivity to a large number of multiplexed sensors. They show negligible thermo-optic and magnetooptic effects in cryogenic environment. These properties make them attractive for temperature surveillance and structural health monitoring of cryogenic systems, or for the testing of material properties and system components at low temperatures.

We report the implementation of an in-fiber optical switch by means of filling a fluid into the air holes of a photonic crystal fiber with a fiber Bragg grating. Such a switch can turn on/off light transmission with an extinction ratio of up to 33 dB within a narrow wavelength range (Bragg wavelength) via a small temperature adjustment of +/-5 degrees C. The switching function is based on the temperature-dependent coupling between the fundamental core mode and the rod modes in the fluid-filled holes resulting from the thermo-optic effect of the filled fluid.

We filled a refractive index matching liquid into the air holes of a Ge-doped solid-core microstructured optical fiber (MOF) with a fiber Bragg grating (FBG) to investigate its switching functions. A type of thermo-optic in-fiber switch based on the tunable bandgap effect was demonstrated in the fluid-filled FBG at the Bragg wavelength of 830nm, and its extinction ratio depends strongly on the reflectivity of the FBG. Another type of optical switch with an extinction ratio of 30 dB was developed in the fluid-filled MOF at a long wavelength of 1200 or 1400nm, attributing to the absorption of the filled liquid. Such two types of switches can turn on/off the light transmission via a small temperature adjustment of ±5 or ±10oC, respectively, and will find useful applications in all-fiber optical communication systems.

Their small size, the multiplexing capability, and the high elasticity of glass fiber can provide reasons to use optical fiber temperature sensors instead of approved conventional thermo couples for temperature monitoring of hot gas turbine components.

An optical switch with a 30dB extinctioin ratio was developed by filling liquid into air holes of a photonic crystal fiber. The switching attributes to the waveguiding change and the absorption of the filled material.