13 results on '"Wireeya Chawjiraphan"'
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2. Effects of Boric Acid and Storage Temperature on the Analysis of Microalbumin Using Aptasensor-Based Fluorescent Detection
- Author
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Chalermwoot Sompark, Wireeya Chawjiraphan, Manatsaphon Sukmak, Ubon Cha’on, Sirirat Anutrakulchai, Prapasiri Pongprayoon, Thitirat Putnin, Dechnarong Pimalai, Visarute Pinrod, and Deanpen Japrung
- Subjects
boric acid ,storage temperature ,microalbumin ,graphene oxide-based fluorescent detection ,Biotechnology ,TP248.13-248.65 - Abstract
The instability of human serum albumin (HSA) in urine samples makes fresh urine a requirement for microalbumin analyses using immunoturbidimetry. Here, we determined the ability of an aptasensor-based fluorescent platform to detect microalbumin in old, boric acid-preserved urine samples. Our results show that the cleavage site of protease enzymes on urine albumin protein differed from the binding position of the aptamer on HSA protein, suggesting the aptasensor may be effective for albumin detection in non-fresh urine. Furthermore, the addition of boric acid in urine samples over a short term (at ambient temperature (Ta) and 4 °C), long term (−20 and −80 °C), and following freeze–thawing (1–3 cycles) did not significantly affect albumin stability, as analyzed using the aptasensor. Therefore, boric acid stabilized has in urine stored over a short- and long-term. Thus, the aptasensor developed by us is applicable for HSA detection in boric acid-preserved urine that has been stored for 7-d at Ta and 4 °C, and in the long-term at −80 °C.
- Published
- 2022
- Full Text
- View/download PDF
3. Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
- Author
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Wireeya Chawjiraphan, Chayachon Apiwat, Khoonsake Segkhoonthod, Kiatnida Treerattrakoon, Preedee Pinpradup, Nuankanya Sathirapongsasuti, Prapasiri Pongprayoon, Patraporn Luksirikul, Patcharee Isarankura-Na-Ayudhya, and Deanpen Japrung
- Subjects
Albuminuria ,Human serum albumin ,Chronic kidney disease ,Aptasensor ,Graphene oxide ,Fluorescence quenching ,Science - Abstract
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1
- Published
- 2020
- Full Text
- View/download PDF
4. Immobilization-Free Electrochemical Sensor Coupled with a Graphene-Oxide-Based Aptasensor for Glycated Albumin Detection
- Author
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Wassa Waiwinya, Thitirat Putnin, Dechnarong Pimalai, Wireeya Chawjiraphan, Nuankanya Sathirapongsasuti, and Deanpen Japrung
- Subjects
graphene oxide ,glycated human serum albumin ,immobilization-free electrochemical measurement ,screen-printed carbon electrode ,diabetes mellitus ,Biotechnology ,TP248.13-248.65 - Abstract
An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin; their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01–50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels.
- Published
- 2021
- Full Text
- View/download PDF
5. Dual sensitive and rapid detection of glycated human serum albumin using a versatile lead/graphene nanocomposite probe as a fluorescence–electrochemical aptasensor
- Author
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Thitirat Putnin, Wassa Waiwinya, Nuankanya Sathirapongsasuti, Dechnarong Pimalai, Deanpen Japrung, and Wireeya Chawjiraphan
- Subjects
Aptamer ,Serum Albumin, Human ,Biosensing Techniques ,02 engineering and technology ,Electrochemistry ,01 natural sciences ,Biochemistry ,Nanocomposites ,Analytical Chemistry ,law.invention ,Limit of Detection ,law ,medicine ,Humans ,Environmental Chemistry ,Lead (electronics) ,Spectroscopy ,Detection limit ,Nanocomposite ,Chromatography ,Graphene ,Chemistry ,010401 analytical chemistry ,Electrochemical Techniques ,Aptamers, Nucleotide ,021001 nanoscience & nanotechnology ,Human serum albumin ,Fluorescence ,0104 chemical sciences ,Lead ,Graphite ,0210 nano-technology ,medicine.drug - Abstract
Monitoring of glycated human serum albumin (GHSA) as a glycemic marker for screening and monitoring of diabetes mellitus is widely practiced for patients with conditions that affect red blood cells. In this study, a complex comprising Pb ions adsorbed on graphene oxide (GO-Pb) was fabricated and utilized as a versatile probe in a fluorescence-electrochemical aptasensor for GHSA quantification. To simplify the aptasensor, the GO-Pb complex probe was prepared via an ion adsorption process. After modification with a fluorophore-labeled aptamer, the GO-Pb complex served as an excellent energy acceptor in fluorescence-based analysis, as well as generating a high current in the electrochemical transducer. Additionally, the proposed platform can detect GHSA via the dual technique from a single sample, allowing for precise and accurate results. Under optimal conditions, the fluorescence-electrochemical aptasensor exhibited a linear relationship with GHSA concentrations from 0.001 to 80 μg mL-1 and from 0.005 to 10 μg mL-1 for fluorescence and electrochemical detection, respectively. The corresponding detection limits were 8.80 ng mL-1 and 0.77 ng mL-1, respectively. The proposed aptasensor additionally displayed good selectivity and excellent stability. Moreover, its successful application in the analysis of clinical samples further demonstrated its utility. Therefore, the proposed platform has significant potential as a novel, facile, highly responsive, and low-cost monitoring method for the development of diabetes mellitus diagnostic devices intended for a clinical setting.
- Published
- 2021
6. THE EFFECTS OF COPRA-MANNO-OLIGOSACCHARIDE ON BACTERIUM AGGREGATION ACTIVITY AND MICROBIOLOGICAL CHANGES IN A SIMULATED DIGESTIVE TRACT.
- Author
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Nawapan Pongsapipatana, Wireeya Chawjiraphan, Suttipun Keawsompong, and Sunee Nitisinprasert
- Subjects
- *
ALIMENTARY canal , *GASTROINTESTINAL system , *MICROBIAL aggregation , *SALMONELLA enteritidis , *ACETIC acid , *POULTRY industry - Abstract
This study aimed to present the interaction of Mannooligosaccharide (MOS) produced by optimum hydrolytic reaction of the recombinant mannanase KMAN treated defatted copra meal (DCM) to bacterial aggregation activity and to microbial changes in simulated gastrointestinal tract which were two main functions of prebiotics as a decoy receptor and a substrate for bacterial fermentation, respectively. The suitable ratio of KMAN and DCM (0.8U:1mg) achieved high DCM hydrolysate-MOS production of 2.13-2.38 g/l during 6-24 h. It enhanced Limosilactobacillus reuteri KUB-AC5 growth for 0.32-0.33 h-1 and accelerated autoaggregation activity by 95.86% and 62.89% at 37 and 42°C, respectively. It decreased autoaggregation activities of Salmonella Enteritidis DMST17368 by 75.03-81.66%, and Lactobacillus johnsonii KUNN19-2 by 38.68-55.63% at 24 h. By simulated human GI model, COPRA-MOS significantly enhanced only Clostridium coccoides-Eubacterium rectale at 24 h with high acetic acid concentration of 87.43 mM. Whereas the chicken simulation model led to low abundance of Enterobacteriaceae but a higher Lactobacillus population with acetic acid concentration of 22.94 mM. These results suggested that COPRA-MOS could potentially apply for poultry industry as it exhibited beneficial effect on cecal fermentation and could be used as a decoy to prevent colonization of a pathogen while to increase the autoaggregation leading to adherence of a probiotic. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
7. THE EFFECTS OF COPRA-MANNO-OLIGOSACCHARIDE ON BACTERIUM AGGREGATION ACTIVITY AND MICROBIOLOGICAL CHANGES IN A SIMULATED DIGESTIVE TRACT
- Author
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Nawapan Pongsapipatana, Wireeya Chawjiraphan, Suttipun Keawsompong, and Sunee Nitisinprasert
- Subjects
Molecular Biology ,Microbiology ,Food Science ,Biotechnology - Abstract
This study aimed to present the interaction of Mannooligosaccharide (MOS) produced by optimum hydrolytic reaction of the recombinant mannanase KMAN treated defatted copra meal (DCM) to bacterial aggregation activity and to microbial changes in simulated gastro-intestinal tract which were two main functions of prebiotics as a decoy receptor and a substrate for bacterial fermentation, respectively. The suitable ratio of KMAN and DCM (0.8U:1mg) achieved high DCM hydrolysate-MOS production of 2.13-2.38 g/l during 6-24 h. It enhanced Limosilactobacillus reuteri KUB-AC5 growth for 0.32-0.33 h-1 and accelerated autoaggregation activity by 95.86% and 62.89% at 37 and 42°C, respectively. It decreased autoaggregation activities of Salmonella Enteritidis DMST17368 by 75.03-81.66%, and Lactobacillus johnsonii KUNN19-2 by 38.68-55.63% at 24 h. By simulated human GI model, COPRA-MOS significantly enhanced only Clostridium coccoides-Eubacterium rectale at 24 h with high acetic acid concentration of 87.43 mM. Whereas the chicken simulation model led to low abundance of Enterobacteriaceae but a higher Lactobacillus population with acetic acid concentration of 22.94 mM. These results suggested that COPRA-MOS could potentially apply for poultry industry as it exhibited beneficial effect on cecal fermentation and could be used as a decoy to prevent colonization of a pathogen while to increase the autoaggregation leading to adherence of a probiotic.
- Published
- 2022
8. Lactococcus lactis KA-FF 1-4 reduces vancomycin-resistant enterococci and impacts the human gut microbiome
- Author
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Sunee Nitisinprasert, Suvimol Charoensiddhi, Massalin Nakphaichit, Sa-Ngapong Plupjeen, and Wireeya Chawjiraphan
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biology ,Lactococcus ,Lactococcus lactis ,Environmental Science (miscellaneous) ,Gut flora ,biology.organism_classification ,Agricultural and Biological Sciences (miscellaneous) ,law.invention ,Microbiology ,Probiotic ,law ,Original Article ,Microbiome ,Bacteroides fragilis ,Bacteria ,Biotechnology ,Bifidobacterium - Abstract
Probiotic is an alternative method to treat intestinal infection disease caused by antibiotic-resistant bacteria. In this study, Lactococcus lactis KA-FF 1-4 demonstrated to have the potential to inhibit the growth of Vancomycin-resistant enterococci (VRE) by producing anti-microbial substance. In co-culture, L. lactis KA-FF 1-4 (10(8) CFU/mL) inhibited the growth of VRE from 10(3)–10(4) CFU/mL to zero after 6 h of exposure. However, in a gut model contained human gut microbiota, this anti-VRE activity of L. lactis KA-FF 1-4 was reduced to only 3.59–6.12%. The unexpected difference in efficacy between the experimental models could be explained by the fact that the growth of L. lactis KA-FF 1-4 was stable in the gut model. Leaving aside these limitations, we observed that adding L. lactis KA-FF 1-4 into the human gut model containing VRE was able to enhance microbial richness and diversity. Specifically, a higher abundance of beneficial microbes from the group of Bifidobacterium spp. and Bacteroides fragilis. L. lactis KA-FF 1-4 also enhanced the abundance of Parabacteroides, Lactococcus, and Fusobacterium and promoted the production of lactic acid in the gut model. However, these effects were not observed in the gut model without L. lactis KA-FF 1-4. Even though this study could not demonstrate a significant anti-VRE effect of the L. lactis KA-FF 1-4 in a gut model, our results still offer evidence that L. lactis KA-FF 1-4 could positively modulate the gut microbiota by promoting the growth of beneficial microbes and their metabolite. L. lactis KA-FF 1-4 has probiotic properties to fight against VRE infection, therefore further investigation in animal model is needed.
- Published
- 2020
9. Immobilization-Free Electrochemical Sensor Coupled with a Graphene-Oxide-Based Aptasensor for Glycated Albumin Detection
- Author
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Thitirat Putnin, Wireeya Chawjiraphan, Deanpen Japrung, Nuankanya Sathirapongsasuti, Wassa Waiwinya, and Dechnarong Pimalai
- Subjects
Glycation End Products, Advanced ,Materials science ,immobilization-free electrochemical measurement ,Calibration curve ,lcsh:Biotechnology ,Clinical Biochemistry ,Oxide ,Biosensing Techniques ,02 engineering and technology ,Electrochemistry ,01 natural sciences ,Article ,law.invention ,chemistry.chemical_compound ,Glycated albumin ,Limit of Detection ,law ,lcsh:TP248.13-248.65 ,glycated human serum albumin ,medicine ,Humans ,Glycated Serum Albumin ,screen-printed carbon electrode ,Serum Albumin ,Detection limit ,Chromatography ,Graphene ,010401 analytical chemistry ,Oxides ,Electrochemical Techniques ,General Medicine ,Aptamers, Nucleotide ,021001 nanoscience & nanotechnology ,Human serum albumin ,0104 chemical sciences ,Electrochemical gas sensor ,chemistry ,diabetes mellitus ,graphene oxide ,Graphite ,0210 nano-technology ,medicine.drug - Abstract
An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin, their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01–50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels.
- Published
- 2021
10. Sensitive detection of albuminuria by graphene oxide-mediated fluorescence quenching aptasensor
- Author
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Patcharee Isarankura-Na-Ayudhya, Preedee Pinpradup, Wireeya Chawjiraphan, Nuankanya Sathirapongsasuti, Prapasiri Pongprayoon, Khoonsake Segkhoonthod, Chayachon Apiwat, Deanpen Japrung, Kiatnida Treerattrakoon, and Patraporn Luksirikul
- Subjects
Aptamer ,Oxide ,Serum Albumin, Human ,Biosensing Techniques ,02 engineering and technology ,Urine ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,Limit of Detection ,law ,medicine ,Albuminuria ,Humans ,Instrumentation ,Spectroscopy ,Detection limit ,Chromatography ,Graphene ,Albumin ,Aptamers, Nucleotide ,021001 nanoscience & nanotechnology ,Human serum albumin ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Spectrometry, Fluorescence ,chemistry ,Graphite ,medicine.symptom ,0210 nano-technology ,medicine.drug - Abstract
Albuminuria is a pathological condition wherein the human serum albumin (HSA) protein is present in abnormally excess amounts in the urine. A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albumin in urine samples and HSA in serum samples. The aptamer-bound HSA used in this aptasensor has hairpin structures, which are characteristic of the aptamer binding site. The limit of detection of the developed platform is 0.05 μg·mL−1 and the detection range is 0.1–14.0 μg·mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with kidney diseases. This approach can be modified to measure HSA using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for other standard automated methods. Therefore, this aptasensor has significant potential for commercialization and wide-scale public use.
- Published
- 2020
11. Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
- Author
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Patraporn Luksirikul, Preedee Pinpradup, Patcharee Isarankura-Na-Ayudhya, Wireeya Chawjiraphan, Khoonsake Segkhoonthod, Nuankanya Sathirapongsasuti, Kiatnida Treerattrakoon, Chayachon Apiwat, Prapasiri Pongprayoon, and Deanpen Japrung
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Aptamer ,Clinical Biochemistry ,Oxide ,Urine ,010501 environmental sciences ,01 natural sciences ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,law ,Chronic kidney disease ,medicine ,Albuminuria ,QD ,lcsh:Science ,Graphene oxide ,030304 developmental biology ,0105 earth and related environmental sciences ,Detection limit ,0303 health sciences ,Chromatography ,Chemistry ,Graphene ,Human serum albumin ,Aptasensor ,Method Article ,Fluorescence quenching ,Medical Laboratory Technology ,lcsh:Q ,medicine.symptom ,Target binding ,medicine.drug - Abstract
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use.•Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample•The limit of detection of our developed platform is 0.05 µg.mL−1•The detection range of our aptasensor is 0.1–600 µg.mL−1, Graphical abstract Image, graphical abstract
- Published
- 2020
12. MULTILOCUS SEQUENCE TYPING OF BRUCELLA ISOLATES FROM THAILAND
- Author
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Wireeya, Chawjiraphan, Piengchan, Sonthayanon, Phanita, Chanket, Surachet, Benjathummarak, Anusak, Kerdsin, and Thareerat, Kalambhaheti
- Subjects
DNA, Bacterial ,Animals ,Genetic Variation ,Humans ,Thailand ,Brucella ,Multiplex Polymerase Chain Reaction ,Phylogeny ,Multilocus Sequence Typing - Abstract
Although brucellosis outbreaks in Thailand are rare, they cause abortions and infertility in animals, resulting in significant economic loss. Because Brucella spp display90% DNA homology, multilocus sequence typing (MLST) was employed to categorize local Brucella isolates into sequence types (STs) and to determine their genetic relatedness. Brucella samples were isolated from vaginal secretion of cows and goats, and from blood cultures of infected individuals. Brucella species were determined by multiplex PCR of eight loci, in addition to MLST based on partial DNA sequences of nine house-keeping genes. MLST analysis of 36 isolates revealed 78 distinct novel allele types and 34 novel STs, while two isolates possessed the known ST8. Sequence alignments identified polymorphic sites in each allele, ranging from 2-6%, while overall genetic diversity was 3.6%. MLST analysis of the 36 Brucella isolates classified them into three species, namely, B. melitensis, B. abortus and B. suis, in agreement with multiplex PCR results. Genetic relatedness among ST members of B. melitensis and B. abortus determined by eBURST program revealed ST2 as founder of B. abortus isolates and ST8 the founder of B. melitensis isolates. ST 36, 41 and 50 of Thai Brucella isolates were identified as single locus variants of clonal cluster (CC) 8, while the majority of STs were diverse. The genetic diversity and relatedness identified using MLST revealed hitherto unexpected diversity among Thai Brucella isolates. Genetic classification of isolates could reveal the route of brucellosis transmission among humans and farm animals and also reveal their relationship with other isolates in the region and other parts of the world.
- Published
- 2018
13. MULTILOCUS SEQUENCE TYPING OF BRUCELLA ISOLATES FROM THAILAND.
- Author
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Wireeya Chawjiraphan, Piengchan Sonthayanon, Phanita Chanket, Surachet Benjathummarak, Anusak Kerdsin, and Thareerat Kalambhaheti
- Published
- 2016
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