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Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
- Source :
- MethodsX, Vol 7, Iss, Pp 101114-(2020), MethodsX
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use.•Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample•The limit of detection of our developed platform is 0.05 µg.mL−1•The detection range of our aptasensor is 0.1–600 µg.mL−1<br />Graphical abstract Image, graphical abstract
- Subjects :
- Aptamer
Clinical Biochemistry
Oxide
Urine
010501 environmental sciences
01 natural sciences
law.invention
03 medical and health sciences
chemistry.chemical_compound
law
Chronic kidney disease
medicine
Albuminuria
QD
lcsh:Science
Graphene oxide
030304 developmental biology
0105 earth and related environmental sciences
Detection limit
0303 health sciences
Chromatography
Chemistry
Graphene
Human serum albumin
Aptasensor
Method Article
Fluorescence quenching
Medical Laboratory Technology
lcsh:Q
medicine.symptom
Target binding
medicine.drug
Subjects
Details
- ISSN :
- 22150161
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- MethodsX
- Accession number :
- edsair.doi.dedup.....34ce85377bc407430772d1bbefb45f4b