70 results on '"Wilkerson DP"'
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2. Sodium bicarbonate ingestion alters the slow but not the fast phase of VO2 kinetics.
- Author
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Berger NJA, McNaughton LR, Keatley S, Wilkerson DP, and Jones AM
- Published
- 2006
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3. Influence of DCA on pulmonary VO2 kinetics during moderate-intensity cycle exercise.
- Author
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Koppo K, Wilkerson DP, Bouckaert J, Wilmshurst S, Campbell IT, and Jones AM
- Abstract
PURPOSE: To test the hypothesis that pharmacological activation of the pyruvate dehydrogenase enzyme complex (PDC) with dichloroacetate (DCA) would speed phase II pulmonary oxygen uptake ((.-)V(O2)) kinetics after the onset of subsequent moderate-intensity (40-45% ((.-)V(O2)) peak) cycle exercise. METHODS: Seven healthy males (mean +/- SD age 25 +/- 4 yr, body mass 75.3 +/- 9.4 kg) performed four 'square-wave' transitions from unloaded cycling to a work rate requiring 90% of the predetermined gas exchange threshold either with or without prior infusion of DCA (50 mg x kg body mass in 50 mL saline). Pulmonary ((.-)V(O2)) was measured breath-by-breath in all tests and ((.-)V(O2)) kinetics were determined from the averaged individual response to each condition using nonlinear regression techniques. RESULTS: The blood [lactate] measured immediately before the onset of exercise was significantly reduced in the DCA condition (C: 1.1 +/- 0.3 vs DCA: 0.6 +/- 0.3 mM; P < 0.01) consistent with successful activation of the PDC. However, DCA had no discernible effect on the rate at which ((.-)V(O2)) increased toward the steady state after the onset of exercise as reflected in the phase II time constant (C: 28.5 +/- 11.8 vs DCA: 29.4 +/- 14.9 s). CONCLUSIONS: The results suggest that PDC activation does not represent a principal intramuscular limitation to ((.-)V(O2)) kinetics after the onset of moderate-intensity exercise. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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4. Prior heavy exercise enhances performance during subsequent perimaximal exercise.
- Author
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Jones AM, Wilkerson DP, Burnley M, and Koppo K
- Abstract
PURPOSE: To test the hypothesis that prior heavy exercise increases the time to exhaustion during subsequent perimaximal exercise. METHODS: Seven healthy males (mean +/- SD 27 +/- 3 yr; 78.4 +/- 0.7 kg) completed square-wave transitions from unloaded cycling to work rates equivalent to 100, 110, and 120% of the work rate at VO2peak (W-[VO2peak) after no prior exercise (control, C) and 10 min after a 6-min bout of heavy exercise at 50% Delta (HE; half-way between the gas exchange threshold (GET) and VO2peak), in a counterbalanced design. RESULTS: Blood [lactate] was significantly elevated before the onset of the perimaximal exercise bouts after prior HE (approximately 2.5 vs approximately 1.1 mM; P < 0.05). Prior HE increased time to exhaustion at 100% (mean +/- SEM. C: 386 +/- 92 vs HE: 613 +/- 161 s), 110% (C: 218 +/- 26 vs HE: 284 +/- 47 s), and 120% (C: 139 +/- 18 vs HE: 180 +/- 29 s) of W-VO2peak, (all P < 0.01). VO2 was significantly higher at 1 min into exercise after prior HE at 110% W-VO2peak (C: 3.11 +/- 0.14 vs HE: 3.42 +/- 0.16 L x min(-1); P < 0.05), and at 1 min into exercise (C: 3.25 +/- 0.12 vs HE: 3.67 +/- 0.15; P < 0.01) and at exhaustion (C: 3.60 +/- 0.08 vs HE: 3.95 +/- 0.12 L x min(-1); P < 0.01) at 120% of W-VO2peak. CONCLUSIONS: This study demonstrate that prior HE, which caused a significant elevation of blood [lactate], resulted in an increased time to exhaustion during subsequent perimaximal exercise presumably by enabling a greater aerobic contribution to the energy requirement of exercise. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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5. .VO2max is not altered by self-pacing during incremental exercise: reply to the letter of Alexis R. Mauger.
- Author
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Chidnok W, Dimenna FJ, Bailey SJ, Burnley M, Wilkerson DP, Vanhatalo A, Jones AM, Chidnok, Weerapong, Dimenna, Fred J, Bailey, Stephen J, Burnley, Mark, Wilkerson, Daryl P, Vanhatalo, Anni, and Jones, Andrew M
- Published
- 2013
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6. Effect of eccentric exercise-induced muscle damage on the dynamics of muscle oxygenation and pulmonary oxygen uptake
- Author
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Ann V. Rowlands, Craig Twist, Andrew M. Jones, Daryl P. Wilkerson, Fred J. DiMenna, David C. Poole, Roger G. Eston, Rosemary C. Davies, Davies, Rosemary, Eston, Roger George, Poole, DC, Rowlands, Alex Viktor, DiMenna, F, Wilkerson, DP, Twist, Craig, and Jones, A.M
- Subjects
Adult ,Male ,Pulmonary Circulation ,medicine.medical_specialty ,Physiology ,Physical exercise ,Microcirculation ,Hemoglobins ,Young Adult ,Oxygen Consumption ,Muscular Diseases ,near-infrared spectroscopy ,oxygen uptake kinetics ,muscle oxygen delivery ,muscle oxygen utilization ,delayed-onset muscle soreness ,Physiology (medical) ,Internal medicine ,Delayed onset muscle soreness ,medicine ,Humans ,Eccentric ,Respiratory system ,Muscle, Skeletal ,Creatine Kinase ,Exercise ,Lung ,Spectroscopy, Near-Infrared ,Chemistry ,Oxygenation ,Anatomy ,Adaptation, Physiological ,Oxygen ,Kinetics ,medicine.anatomical_structure ,Eccentric exercise ,Cardiology ,Human Movement and Sports Science ,medicine.symptom ,Biomarkers - Abstract
Unaccustomed eccentric exercise has a profound impact on muscle structure and function. However, it is not known whether associated microvascular dysfunction disrupts the matching of O2delivery (Q̇o2) to O2utilization (V̇o2). Near-infrared spectroscopy (NIRS) was used to test the hypothesis that eccentric exercise-induced muscle damage would elevate the muscle Q̇o2:V̇o2ratio during severe-intensity exercise while preserving the speed of the V̇o2kinetics at exercise onset. Nine physically active men completed “step” tests to severe-intensity exercise from an unloaded baseline on a cycle ergometer before (Pre) and 48 h after (Post) eccentric exercise (100 squats with a load corresponding to 70% of body mass). NIRS and breath-by-breath pulmonary V̇o2were measured continuously during the exercise tests and subsequently modeled using standard nonlinear regression techniques. There were no changes in phase II pulmonary V̇o2kinetics following the onset of exercise (time constant: Pre, 25 ± 4 s; Post, 24 ± 2 s; amplitude: Pre, 2.36 ± 0.23 l/min; Post, 2.37 ± 0.23 l/min; all P > 0.05). However, the primary (Pre, 14 ± 3 s; Post, 19 ± 3 s) and overall (Pre, 16 ± 4 s; Post, 21 ± 4 s) mean response time of the [HHb] response was significantly slower following eccentric exercise ( P < 0.05). The slower [HHb] kinetics observed following eccentric exercise is consistent with an increased Q̇o2:V̇o2ratio during transitions to severe-intensity exercise. We propose that unchanged primary phase V̇o2kinetics are associated with an elevated Q̇o2:V̇o2ratio that preserves blood-myocyte O2flux.
- Published
- 2008
7. Effects of dietary nitrate on the O 2 cost of submaximal exercise: Accounting for "noise" in pulmonary gas exchange measurements.
- Author
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Tan R, Wylie LJ, Wilkerson DP, Vanhatalo A, and Jones AM
- Subjects
- Cross-Over Studies, Dietary Supplements, Double-Blind Method, Exercise Tolerance physiology, Humans, Nitrites, Oxygen, Oxygen Consumption physiology, Pulmonary Gas Exchange, Beta vulgaris, Nitrates
- Abstract
Dietary nitrate (NO
3 - ) supplementation can reduce the oxygen cost of submaximal exercise, but this has not been reported consistently. We hypothesised that the number of step transitions to moderate-intensity exercise, and corresponding effects on the signal-to-noise ratio for pulmonary V ˙ O2 , may be important in this regard. Twelve recreationally active participants were assigned in a randomised, double-blind, crossover design to supplement for 4 days in three conditions: 1) control (CON; water); 2); PL (NO3 - -depleted beetroot juice); and 3) BR (NO3 - -rich beetroot juice). On days 3 and 4, participants completed two 6-min step transitions to moderate-intensity cycle exercise. Breath-by-breath V ˙ O2 data were collected and V ˙ O2 kinetic responses were determined for a single transition and when the responses to 2, 3 and 4 transitions were ensemble-averaged. Steady-state V ˙ O2 was not different between PL and BR when the V ˙ O2 response to one-, two- or three-step transition was compared but was significantly lower in BR compared to PL when four-step transitions was considered (PL: 1.33 ± 0.34 vs. BR: 1.31 ± 0.34 L·min-1 , P < 0.05). There were no differences in pulmonary V ˙ O2 responses between CON and PL ( P > 0.05). Multiple step transitions may be required to detect the influence of NO3 - supplementation on steady-state V ˙ O2 .- Published
- 2022
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8. Physiological demands of running at 2-hour marathon race pace.
- Author
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Jones AM, Kirby BS, Clark IE, Rice HM, Fulkerson E, Wylie LJ, Wilkerson DP, Vanhatalo A, and Wilkins BW
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- Adult, Athletes, Exercise Test, Humans, Lactic Acid, Male, Physical Endurance, Marathon Running, Oxygen Consumption
- Abstract
The requirements of running a 2-h marathon have been extensively debated but the actual physiological demands of running at ∼21.1 km/h have never been reported. We therefore conducted laboratory-based physiological evaluations and measured running economy (O
2 cost) while running outdoors at ∼21.1 km/h, in world-class distance runners as part of Nike's "Breaking 2" marathon project. On separate days, 16 world-class male distance runners (age, 29 ± 4 yr; height, 1.72 ± 0.04 m; mass, 58.9 ± 3.3 kg) completed an incremental treadmill test for the assessment of V̇O2peak , O2 cost of submaximal running, lactate threshold and lactate turn-point, and a track test during which they ran continuously at 21.1 km/h. The laboratory-determined V̇O2peak was 71.0 ± 5.7 mL/kg/min with lactate threshold and lactate turn-point occurring at 18.9 ± 0.4 and 20.2 ± 0.6 km/h, corresponding to 83 ± 5% and 92 ± 3% V̇O2peak , respectively. Seven athletes were able to attain a steady-state V̇O2 when running outdoors at 21.1 km/h. The mean O2 cost for these athletes was 191 ± 19 mL/kg/km such that running at 21.1 km/h required an absolute V̇O2 of ∼4.0 L/min and represented 94 ± 3% V̇O2peak . We report novel data on the O2 cost of running outdoors at 21.1 km/h, which enables better modeling of possible marathon performances by elite athletes. Using the value for O2 cost measured in this study, a sub 2-h marathon would require a 59 kg runner to sustain a V̇O2 of approximately 4.0 L/min or 67 mL/kg/min. NEW & NOTEWORTHY We report the physiological characteristics and O2 cost of running overground at ∼21.1 km/h in a cohort of the world's best male distance runners. We provide new information on the absolute and relative O2 uptake required to run at 2-h marathon pace.- Published
- 2021
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9. Effect of nitrate supplementation on hepatic blood flow and glucose homeostasis: a double-blind, placebo-controlled, randomized control trial.
- Author
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Shepherd AI, Wilkerson DP, Fulford J, Winyard PG, Benjamin N, Shore AC, and Gilchrist M
- Subjects
- Adult, Aged, Blood Flow Velocity drug effects, Double-Blind Method, Female, Humans, Male, Middle Aged, Nitric Oxide metabolism, Portal Vein drug effects, Portal Vein physiology, Young Adult, Blood Glucose drug effects, Liver blood supply, Nitrates administration & dosage, Nitrates pharmacology
- Abstract
Nitric oxide alters gastric blood flow, improves vascular function, and mediates glucose uptake within the intestines and skeletal muscle. Dietary nitrate, acting as a source of nitric oxide, appears to be a potential low-cost therapy that may help maintain glucose homeostasis. In a randomized, double-blind, placebo-controlled crossover study, 31 young and older adult participants had a standardized breakfast, supplemented with either nitrate-rich beetroot juice (11.91 mmol nitrate) or nitrate-depleted beetroot juice as placebo (0.01 mmol nitrate). MRI was used to assess apparent diffusion coefficient (ADC), portal vein flux, and velocity. Plasma glucose, incretin, and C-peptide concentrations and blood pressure were assessed. Outcome variables were measured at baseline and hourly for 3 h. Compared with a placebo, beetroot juice resulted in a significant elevation in plasma nitrate and plasma nitrite concentration. No differences were seen for the young or older adult cohorts between placebo and beetroot juice for ADC, or portal vein flux. There was an interaction effect in the young adults between visits for portal vein velocity. Nitrate supplementation did not reduce plasma glucose, active GLP-1, total GLP-1, or plasma C-peptide concentrations for the young or older adult cohorts. Despite a significant elevation in plasma nitrite concentration following an acute dose of (11.91 mmol) nitrate, there was no effect on hepatic blood flow, plasma glucose, C-peptide, or incretin concentration in healthy adults., (Copyright © 2016 the American Physiological Society.)
- Published
- 2016
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10. Effects of dietary nitrate supplementation on the oxygen cost of exercise and walking performance in individuals with type 2 diabetes: a randomized, double-blind, placebo-controlled crossover trial.
- Author
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Shepherd AI, Gilchrist M, Winyard PG, Jones AM, Hallmann E, Kazimierczak R, Rembialkowska E, Benjamin N, Shore AC, and Wilkerson DP
- Subjects
- Aged, Antioxidants analysis, Beta vulgaris chemistry, Combined Modality Therapy, Cross-Over Studies, Diabetes Mellitus, Type 2 metabolism, Dietary Supplements, Double-Blind Method, Exercise Therapy, Female, Fruit and Vegetable Juices analysis, Humans, Male, Middle Aged, Oxygen Consumption, Treatment Outcome, Walking, Diabetes Mellitus, Type 2 therapy, Nitrates administration & dosage
- Abstract
Dietary nitrate supplementation has been shown to reduce the oxygen (O2) cost of exercise and enhance exercise tolerance in healthy individuals. This study assessed whether similar effects could be observed in individuals with type 2 diabetes (T2DM). In a randomized, double-blind, placebo-controlled crossover study, 48 participants with T2DM supplemented their diet for 4 days with either nitrate-rich beetroot juice (70ml/day, 6.43mmol nitrate/day) or nitrate-depleted beetroot juice as placebo (70ml/day, 0.07mmol nitrate/day). After each intervention period, resting plasma nitrate and nitrite concentrations were measured subsequent to participants completing moderate-paced walking. Pulmonary gas exchange was measured to assess the O2 cost of walking. After a rest period, participants performed the 6-min walk test (6MWT). Relative to placebo, beetroot juice resulted in a significant increase in plasma nitrate (placebo, 57±66 vs beetroot, 319±110µM; P < 0.001) and plasma nitrite concentration (placebo, 680±256 vs beetroot, 1065±607nM; P < 0.001). There were no differences between placebo juice and beetroot juice for the O2 cost of walking (946±221 vs 939±223ml/min, respectively; P = 0.59) and distance covered in the 6MWT (550±83 vs 554±90m, respectively; P = 0.17). Nitrate supplementation did not affect the O2 cost of moderate-paced walking or improve performance in the 6MWT. These findings indicate that dietary nitrate supplementation does not modulate the response to exercise in individuals with T2DM., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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11. The effect of dietary nitrate supplementation on the oxygen cost of cycling, walking performance and resting blood pressure in individuals with chronic obstructive pulmonary disease: A double blind placebo controlled, randomised control trial.
- Author
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Shepherd AI, Wilkerson DP, Dobson L, Kelly J, Winyard PG, Jones AM, Benjamin N, Shore AC, and Gilchrist M
- Subjects
- Adult, Aged, Beta vulgaris, Dietary Supplements, Double-Blind Method, Humans, Middle Aged, Nitrates blood, Oxygen metabolism, Pulmonary Disease, Chronic Obstructive physiopathology, Pulmonary Gas Exchange, Walking, Blood Pressure drug effects, Exercise physiology, Nitrates pharmacology, Pulmonary Disease, Chronic Obstructive diet therapy
- Abstract
Background: Chronic obstructive pulmonary disease (COPD) results in exercise intolerance. Dietary nitrate supplementation has been shown to lower blood pressure (BP), reduce the oxygen cost of exercise, and enhance exercise tolerance in healthy volunteers. This study assessed the effects of dietary nitrate on the oxygen cost of cycling, walking performance and BP in individuals with mild-moderate COPD., Methods: Thirteen patients with mild-moderate COPD were recruited. Participants consumed 70 ml of either nitrate-rich (6.77 mmol nitrate; beetroot juice) or nitrate-depleted beetroot juice (0.002 mmol nitrate; placebo) twice a day for 2.5 days, with the final supplement ~3 hours before testing. BP was measured before completing two bouts of moderate-intensity cycling, where pulmonary gas exchange was measured throughout. The six-minute walk test (6 MWT) was completed 30 minutes subsequent to the second cycling bout., Results: Plasma nitrate concentration was significantly elevated following beetroot juice vs. placebo (placebo; 48 ± 86 vs. beetroot juice; 215 ± 84 µM, P = 0.002). No significant differences were observed between placebo vs. beetroot juice for oxygen cost of exercise (933 ± 323 vs. 939 ± 302 ml: min(-1); P = 0.88), distance covered in the 6 MWT (456 ± 86 vs. 449 ± 79 m; P = 0.37), systolic BP (123 ± 14 vs. 123 ± 14 mmHg; P = 0.91), or diastolic BP (77 ± 9 vs. 79 ± 9 mmHg; P = 0.27)., Conclusion: Despite a large rise in plasma nitrate concentration, two days of nitrate supplementation did not reduce the oxygen cost of moderate intensity cycling, increase distance covered in the 6 MWT, or lower BP., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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12. Unchanged content of oxidative enzymes in fast-twitch muscle fibers and V˙O2 kinetics after intensified training in trained cyclists.
- Author
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Christensen PM, Gunnarsson TP, Thomassen M, Wilkerson DP, Nielsen JJ, and Bangsbo J
- Abstract
The present study examined if high intensity training (HIT) could increase the expression of oxidative enzymes in fast-twitch muscle fibers causing a faster oxygen uptake (V˙O2) response during intense (INT), but not moderate (MOD), exercise and reduce the V˙O2 slow component and muscle metabolic perturbation during INT. Pulmonary V˙O2 kinetics was determined in eight trained male cyclists (V˙O2-max: 59 ± 4 (means ± SD) mL min(-1) kg(-1)) during MOD (205 ± 12 W ~65% V˙O2-max) and INT (286 ± 17 W ~85% V˙O2-max) exercise before and after a 7-week HIT period (30-sec sprints and 4-min intervals) with a 50% reduction in volume. Both before and after HIT the content in fast-twitch fibers of CS (P < 0.05) and COX-4 (P < 0.01) was lower, whereas PFK was higher (P < 0.001) than in slow-twitch fibers. Content of CS, COX-4, and PFK in homogenate and fast-twitch fibers was unchanged with HIT. Maximal activity (μmol g DW(-1) min(-1)) of CS (56 ± 8 post-HIT vs. 59 ± 10 pre-HIT), HAD (27 ± 6 vs. 29 ± 3) and PFK (340 ± 69 vs. 318 ± 105) and the capillary to fiber ratio (2.30 ± 0.16 vs. 2.38 ± 0.20) was unaltered following HIT. V˙O2 kinetics was unchanged with HIT and the speed of the primary response did not differ between MOD and INT. Muscle creatine phosphate was lower (42 ± 15 vs. 66 ± 17 mmol kg DW(-1)) and muscle lactate was higher (40 ± 18 vs. 14 ± 5 mmol kg DW(-1)) at 6 min of INT (P < 0.05) after compared to before HIT. A period of intensified training with a volume reduction did not increase the content of oxidative enzymes in fast-twitch fibers, and did not change V˙O2 kinetics., (© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.)
- Published
- 2015
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13. Effects of nitrate on the power-duration relationship for severe-intensity exercise.
- Author
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Kelly J, Vanhatalo A, Wilkerson DP, Wylie LJ, and Jones AM
- Subjects
- Adult, Beta vulgaris, Bicycling physiology, Cross-Over Studies, Dietary Supplements, Double-Blind Method, Exercise Tolerance physiology, Humans, Male, Physical Endurance physiology, Plant Roots, Young Adult, Exercise Tolerance drug effects, Nitrates pharmacology, Physical Endurance drug effects, Physical Exertion physiology, Plant Extracts pharmacology
- Abstract
Purpose: The power asymptote (critical power [CP]) and curvature constant (W') of the power-duration relationship dictate the tolerance to severe-intensity exercise. We tested the hypothesis that dietary nitrate supplementation would increase the CP and/or the W' during cycling exercise., Methods: In a double-blind, randomized, crossover study, nine recreationally active male subjects supplemented their diet with either nitrate-rich concentrated beetroot juice (BR; 2 × 250 mL·d, ∼8.2 mmol·d nitrate) or a nitrate-depleted BR placebo (PL; 2 × 250 mL·d, ∼0.006 mmol·d nitrate). In each condition, the subjects completed four separate severe-intensity exercise bouts to exhaustion at 60% of the difference between the gas exchange threshold and the peak power attained during incremental exercise (60% Δ), 70% Δ, 80% Δ, and 100% peak power, and the results were used to establish CP and W'., Results: Nitrate supplementation improved exercise tolerance during exercise at 60% Δ (BR, 696 ± 120 vs PL, 593 ± 68 s; P < 0.05), 70% Δ (BR, 452 ± 106 vs PL, 390 ± 86 s; P < 0.05), and 80% Δ (BR, 294 ± 50 vs PL, 263 ± 50 s; P < 0.05) but not 100% peak power (BR, 182 ± 37 vs PL, 166 ± 26 s; P = 0.10). Neither CP (BR, 221 ± 27 vs PL, 218 ± 26 W) nor W' (BR, 19.3 ± 4.6 vs PL, 17.8 ± 3 kJ) were significantly altered by BR., Conclusion: Dietary nitrate supplementation improved endurance during severe-intensity exercise in recreationally active subjects without significantly increasing either the CP or the W'.
- Published
- 2013
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14. Effects of pacing strategy on work done above critical power during high-intensity exercise.
- Author
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Chidnok W, Dimenna FJ, Bailey SJ, Wilkerson DP, Vanhatalo A, and Jones AM
- Subjects
- Adult, Electromyography, Exercise Test, Fatigue etiology, Humans, Male, Bicycling physiology, Oxygen Consumption physiology, Physical Endurance physiology, Physical Exertion physiology
- Abstract
Purpose: We investigated the influence of pacing strategy on the work completed above critical power (CP) before exhaustion (W>CP) and the peak V˙O2 attained during high-intensity cycling., Methods: After the determination of VO(2max) from a ramp incremental cycling (INC) test and the estimation of the parameters of the power-duration relationship for high-intensity exercise (i.e., CP and W') from a 3-min all-out cycling test (AOT), eight male subjects completed a cycle test to exhaustion at a severe-intensity constant work rate (CWR) estimated to result in exhaustion in 3 min and a self-paced 3-min cycling time trial (SPT)., Results: The VO(2max) determined from INC was 4.24 ± 0.69 L · min(-1), and the CP and the W' estimated from AOT were 260 ± 60 W and 16.5 ± 4.0 kJ, respectively. W>CP during SPT was not significantly different from W>CP during CWR (15.3 ± 5.6 and 16.6 ± 7.4 kJ, respectively), and these values were also similar to W(>CP) during INC (16.4 ± 4.0 kJ) and W' estimated from AOT. The peak VO(2) during SPT was not significantly different from peak VO(2) during CWR (4.20 ± 0.77 and 4.14 ± 0.75 L · min(-1), respectively), and these values were similar to the VO(2max) determined from INC and the peak VO(2) during AOT (4.10 ± 0.79 L · min(-1))., Conclusion: Exhaustion during high-intensity exercise coincides with the achievement of the same peak VO2 (VO(2max)) and the completion of the same W>CP, irrespective of the work rate forcing function (INC or CWR) or pacing strategy (enforced pace or self-paced). These findings indicate that exhaustion during high-intensity exercise is based on highly predictable physiological processes, which are unaffected when pacing strategy is self-selected.
- Published
- 2013
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15. Influence of acute dietary nitrate supplementation on 50 mile time trial performance in well-trained cyclists.
- Author
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Wilkerson DP, Hayward GM, Bailey SJ, Vanhatalo A, Blackwell JR, and Jones AM
- Subjects
- Adult, Beta vulgaris chemistry, Beverages, Bicycling, Case-Control Studies, Humans, Male, Nitrates blood, Oxygen Consumption, Physical Endurance physiology, Dietary Supplements, Nitrates pharmacology, Physical Endurance drug effects
- Abstract
Dietary nitrate supplementation has been reported to improve short distance time trial (TT) performance by 1-3 % in club-level cyclists. It is not known if these ergogenic effects persist in longer endurance events or if dietary nitrate supplementation can enhance performance to the same extent in better trained individuals. Eight well-trained male cyclists performed two laboratory-based 50 mile TTs: (1) 2.5 h after consuming 0.5 L of nitrate-rich beetroot juice (BR) and (2) 2.5 h after consuming 0.5 L of nitrate-depleted BR as a placebo (PL). BR significantly elevated plasma [NO(2) (-)] (BR: 472 ± 96 vs. PL: 379 ± 94 nM; P < 0.05) and reduced completion time for the 50 mile TT by 0.8 % (BR: 136.7 ± 5.6 vs. PL: 137.9 ± 6.4 min), which was not statistically significant (P > 0.05). There was a significant correlation between the increased post-beverage plasma [NO(2) (-)] with BR and the reduction in TT completion time (r = -0.83, P = 0.01). Power output (PO) was not different between the conditions at any point (P > 0.05) but oxygen uptake ([Formula: see text]O(2)) tended to be lower in BR (P = 0.06), resulting in a significantly greater PO/[Formula: see text]O(2) ratio (BR: 67.4 ± 5.5 vs. PL: 65.3 ± 4.8 W L min(-1); P < 0.05). In conclusion, acute dietary supplementation with beetroot juice did not significantly improve 50 mile TT performance in well-trained cyclists. It is possible that the better training status of the cyclists in this study might reduce the physiological and performance response to NO(3) (-) supplementation compared with the moderately trained cyclists tested in earlier studies.
- Published
- 2012
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16. Influence of passive lower-body heating on muscle metabolic perturbation and high-intensity exercise tolerance in humans.
- Author
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Bailey SJ, Wilkerson DP, Fulford J, and Jones AM
- Subjects
- Adult, Body Temperature physiology, Heart Rate physiology, Humans, Male, Oxygen Consumption physiology, Body Temperature Regulation physiology, Exercise physiology, Exercise Tolerance physiology, Muscle, Skeletal physiology
- Abstract
The purpose of this investigation was to determine the influence of heat stress on the dynamics of muscle metabolic perturbation during high-intensity exercise. Seven healthy males completed single-legged knee-extensor exercise until the limit of tolerance on two separate occasions. In a randomized order the subjects underwent 40 min of lower-body immersion in warm water at 42°C prior to exercise (HOT) or received no prior thermal manipulation (CON). Following the intervention, muscle metabolism was measured at rest and throughout exercise using (31)P-MRS. The tolerable duration of high-intensity exercise was reduced by 36% after passive heating (CON: 474 ± 146 vs. HOT: 303 ± 76 s; P = 0.005). Intramuscular pH was lower over the first 60 s of exercise (CON: 7.05 ± 0.02 vs. HOT: 7.00 ± 0.03; P = 0.019) in HOT compared to CON. The rate of muscle [PCr] degradation during exercise was greater in the HOT condition (CON: -0.17 ± 0.08 vs. HOT: -0.25 ± 0.10% s(-1); P = 0.006) and pH also tended to change more rapidly in HOT (P = 0.09). Muscle [PCr] (CON: 26 ± 14 vs. HOT: 29 ± 10%), [Pi] (CON: 504 ± 236 vs. HOT: 486 ± 186%) and pH (CON: 6.84 ± 0.13 vs. HOT: 6.80 ± 0.14; P > 0.05) were not statistically different at the limit of tolerance (P > 0.05 for all comparisons). These results suggest that the reduced time-to-exhaustion during high-intensity knee-extensor exercise following lower-body heating might be related, in part, to accelerated rates of change of intramuscular [PCr] and pH towards 'critical' values that limit muscle function.
- Published
- 2012
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17. Influence of exercise intensity on skeletal muscle blood flow, O2 extraction and O2 uptake on-kinetics.
- Author
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Jones AM, Krustrup P, Wilkerson DP, Berger NJ, Calbet JA, and Bangsbo J
- Subjects
- Adult, Exercise Test, Humans, Kinetics, Male, Oxygen blood, Oxygen Consumption, Young Adult, Exercise physiology, Muscle, Skeletal blood supply, Muscle, Skeletal metabolism
- Abstract
Following the start of low-intensity exercise in healthy humans, it has been established that the kinetics of skeletal muscle O(2) delivery is faster than, and does not limit, the kinetics of muscle O(2) uptake (V(O(2)(m))). Direct data are lacking, however, on the question of whether O(2) delivery might limit (V(O(2)(m))) kinetics during high-intensity exercise. Using multiple exercise transitions to enhance confidence in parameter estimation, we therefore investigated the kinetics of, and inter-relationships between, muscle blood flow (Q(m)), a-(V(O(2))) difference and (V(O(2)(m))) following the onset of low-intensity (LI) and high-intensity (HI) exercise. Seven healthy males completed four 6 min bouts of LI and four 6 min bouts of HI single-legged knee-extension exercise. Blood was frequently drawn from the femoral artery and vein during exercise and Q(m), a-(V(O(2))) difference and (V(O(2)(m))) were calculated and subsequently modelled using non-linear regression techniques. For LI, the fundamental component mean response time (MRT(p)) for Q(m) kinetics was significantly shorter than (V(O(2)(m))) kinetics (mean ± SEM, 18 ± 4 vs. 30 ± 4 s; P < 0.05), whereas for HI, the MRT(p) for Q(m) and (V(O(2)(m))) was not significantly different (27 ± 5 vs. 29 ± 4 s, respectively). There was no difference in the MRT(p) for either Q(m) or (V(O(2)(m))) between the two exercise intensities; however, the MRT(p)for a-(V(O(2)) difference was significantly shorter for HI compared with LI (17 ± 3 vs. 28 ± 4 s; P < 0.05). Excess O(2), i.e. oxygen not taken up (Q(m) x (V(O(2))), was significantly elevated within the first 5 s of exercise and remained unaltered thereafter, with no differences between LI and HI. These results indicate that bulk O(2) delivery does not limit (V(O(2)(m))) kinetics following the onset of LI or HI knee-extension exercise.
- Published
- 2012
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18. Influence of initial metabolic rate on the power-duration relationship for all-out exercise.
- Author
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Parker Simpson L, Jones AM, Vanhatalo A, and Wilkerson DP
- Subjects
- Adult, Computer Simulation, Humans, Male, Metabolic Clearance Rate, Energy Transfer physiology, Models, Biological, Oxygen physiology, Oxygen Consumption physiology, Physical Endurance physiology
- Abstract
A single 3-min all-out cycling test can be used to estimate the power asymptote (critical power, CP) and the curvature constant (W') of the power-duration relationship for severe-intensity exercise. It was hypothesized that when exercise immediately preceding the 3-min all-out test was performed
CP would systematically reduce the W' without affecting the CP. Seven physically active males completed 3-min all-out cycling tests in randomized order immediately preceded by: unloaded cycling (control); 6-min moderate; 6-min heavy; 2-min severe (S2); or 4-min severe (S4) intensity exercise. The CP was estimated from the mean power output over the final 30 s of the test and the W' was estimated as the power-time integral above end-test power. There were no significant differences in the CP between control (279 ± 62), moderate (275 ± 52), heavy (286 ± 66 W), S2 (274 ± 55), or S4 (273 ± 65 W). The W' was significantly lower (P < 0.05) in S2 (11.5 ± 2.5) and S4 (8.9 ± 2.2) than in control (16.3 ± 2.3), moderate (17.2 ± 2.4) and heavy (15.6 ± 2.3 kJ). These results support the notion that the W' is predictably depleted only at a power output >CP whereas the CP is independent of the mechanisms which reduce W'. - Published
- 2012
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- View/download PDF
19. Exercise tolerance in intermittent cycling: application of the critical power concept.
- Author
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Chidnok W, Dimenna FJ, Bailey SJ, Vanhatalo A, Morton RH, Wilkerson DP, and Jones AM
- Subjects
- Adult, Analysis of Variance, Electromyography, Exercise Test, Humans, Male, Oxygen Consumption physiology, Pulmonary Gas Exchange, Bicycling physiology, Exercise Tolerance physiology, Recovery of Function physiology
- Abstract
Purpose: This study tested the relevance of the critical power (CP) model for explaining exercise tolerance during intermittent high-intensity exercise with different recovery intensities., Methods: After estimation of CP and W' from a 3-min all-out test, seven male subjects completed, in randomized order, a cycle test to exhaustion at a severe-intensity constant-work-rate (S-CWR) and four cycle tests to exhaustion using different intermittent ("work-recovery") protocols (i.e., severe-severe (S-S), severe-heavy (S-H), severe-moderate (S-M), and severe-light (S-L))., Results: The tolerable duration of exercise in S-CWR was 384 ± 48 s, and this was increased by 47%, 100%, and 219% for S-H, S-M, and S-L, respectively (all P < 0.05). Consistent with this, compared with S-CWR (22.9 ± 7.4 kJ), the work done above the CP was significantly greater by 46%, 98%, and 220% for S-H, S-M, and S-L, respectively (all P < 0.05). The slope of the relationship between V˙O₂ and time was significantly reduced for S-H, S-M, and S-L (0.09 ± 0.02, 0.09 ± 0.01, and 0.07 ± 0.02 L·min⁻², respectively) compared with S-CWR (0.16 ± 0.03 L·min⁻², P < 0.05). In addition, the slope of the relationship between integrated EMG and time showed a systematic decline for S-H, S-M, and S-L compared with S-CWR (P < 0.05)., Conclusions: These results indicate that, when recovery intervals during intermittent exercise are performed below the CP, exercise tolerance is improved in proportion to the reconstitution of the finite W'. The enhanced exercise tolerance with the lower-intensity recovery intervals was associated with a blunted increase in both V˙O₂ and integrated EMG with time.
- Published
- 2012
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- View/download PDF
20. Acute dietary nitrate supplementation improves cycling time trial performance.
- Author
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Lansley KE, Winyard PG, Bailey SJ, Vanhatalo A, Wilkerson DP, Blackwell JR, Gilchrist M, Benjamin N, and Jones AM
- Subjects
- Beverages, Blood Pressure, Cross-Over Studies, Humans, Male, Nitrites blood, Young Adult, Athletic Performance physiology, Bicycling physiology, Dietary Supplements, Nitrates therapeutic use
- Abstract
Purpose: Dietary nitrate supplementation has been shown to reduce the O2 cost of submaximal exercise and to improve high-intensity exercise tolerance. However, it is presently unknown whether it may enhance performance during simulated competition. The present study investigated the effects of acute dietary nitrate supplementation on power output (PO), VO2, and performance during 4- and 16.1-km cycling time trials (TT)., Methods: After familiarization, nine club-level competitive male cyclists were assigned in a randomized, crossover design to consume 0.5 L of beetroot juice (BR; containing ∼ 6.2 mmol of nitrate) or 0.5 L of nitrate-depleted BR (placebo, PL; containing ∼ 0.0047 mmol of nitrate), ∼ 2.5 h before the completion of a 4- and a 16.1-km TT., Results: BR supplementation elevated plasma [nitrite] (PL = 241 ± 125 vs BR = 575 ± 199 nM, P < 0.05). The VO2 values during the TT were not significantly different between the BR and PL conditions at any elapsed distance (P > 0.05), but BR significantly increased mean PO during the 4-km (PL = 279 ± 51 vs BR = 292 ± 44 W, P < 0.05) and 16.1-km TT (PL = 233 ± 43 vs BR = 247 ± 44 W, P < 0.01). Consequently, BR improved 4-km performance by 2.8% (PL = 6.45 ± 0.42 vs BR = 6.27 ± 0.35 min, P < 0.05) and 16.1-km performance by 2.7% (PL = 27.7 ± 2.1 vs BR = 26.9 ± 1.8 min, P < 0.01)., Conclusions: These results suggest that acute dietary nitrate supplementation with 0.5 L of BR improves cycling economy, as demonstrated by a higher PO for the same VO2 and enhances both 4- and 16.1-km cycling TT performance.
- Published
- 2011
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21. Fast-start strategy improves VO2 kinetics and high-intensity exercise performance.
- Author
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Bailey SJ, Vanhatalo A, DiMenna FJ, Wilkerson DP, and Jones AM
- Subjects
- Exercise Test, Heart Rate physiology, Humans, Lactic Acid blood, Male, Muscle, Skeletal physiology, Oxygen physiology, Pulmonary Gas Exchange physiology, Young Adult, Athletic Performance physiology, Exercise physiology, Oxygen Consumption physiology
- Abstract
Purpose: The purpose of this study was to investigate the influence of pacing strategy on pulmonary VO2 kinetics and performance during high-intensity exercise., Methods: Seven males completed 3- and 6-min bouts of cycle exercise on three occasions with the bouts initiated using an even-start (ES; constant work rate), fast-start (FS), or slow-start (SS) pacing strategy. In all conditions, subjects completed an all-out sprint over the final 60 s of the test as a measure of performance., Results: For the 3-min exercise bouts, the mean response time (MRT) for the VO2 kinetics over the pacing phase was shortest in FS (35 ± 6 s), longest in SS (55 ± 14 s), and intermediate in ES (41 ± 10 s) (P < 0.05 for all comparisons). For the 6-min bouts, the VO2 MRT was longer in SS (56 ± 15 s) than that in FS and ES (38 ± 7 and 42 ± 6 s, respectively, P < 0.05). The VO2 at the end of exercise was not different from the VO2max during the 6-min exercise bouts or 3-FS but was lower than VO2max for 3-ES and 3-SS (P < 0.05). The end-sprint performance was significantly enhanced in 3-FS compared with 3-ES and 3-SS (mean power = 374 ± 68 vs 348 ± 61 and 345 ± 71 W, respectively; P < 0.05). However, end-sprint performance was unaffected by pacing strategy in the 6-min bouts., Conclusions: These data indicate that an FS pacing strategy significantly improves performance during 3-min bouts of high-intensity exercise by speeding VO2 kinetics and enabling the attainment of VO2max.
- Published
- 2011
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22. Older type 2 diabetic males do not exhibit abnormal pulmonary oxygen uptake and muscle oxygen utilization dynamics during submaximal cycling exercise.
- Author
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Wilkerson DP, Poole DC, Jones AM, Fulford J, Mawson DM, Ball CI, and Shore AC
- Subjects
- Adaptation, Physiological, Age Factors, Aged, Case-Control Studies, Diabetes Mellitus, Type 2 physiopathology, Hemoglobins metabolism, Humans, Kinetics, Male, Middle Aged, Muscle, Skeletal blood supply, Muscle, Skeletal physiopathology, Regional Blood Flow, Bicycling, Diabetes Mellitus, Type 2 metabolism, Exercise, Muscle Contraction, Muscle, Skeletal metabolism, Oxygen Consumption, Pulmonary Gas Exchange
- Abstract
There are reports of abnormal pulmonary oxygen uptake (Vo(2)) and deoxygenated hemoglobin ([HHb]) kinetics in individuals with Type 2 diabetes (T2D) below 50 yr of age with disease durations of <5 yr. We examined the Vo(2) and muscle [HHb] kinetics in 12 older T2D patients with extended disease durations (age: 65 ± 5 years; disease duration 9.3 ± 3.8 years) and 12 healthy age-matched control participants (CON; age: 62 ± 6 years). Maximal oxygen uptake (Vo(2max)) was determined via a ramp incremental cycle test and Vo(2) and [HHb] kinetics were determined during subsequent submaximal step exercise. The Vo(2max) was significantly reduced (P < 0.05) in individuals with T2D compared with CON (1.98 ± 0.43 vs. 2.72 ± 0.40 l/min, respectively) but, surprisingly, Vo(2) kinetics was not different in T2D compared with CON (phase II time constant: 43 ± 17 vs. 41 ± 12 s, respectively). The Δ[HHb]/ΔVo(2) was significantly higher in T2D compared with CON (235 ± 99 vs. 135 ± 33 AU·l(-1)·min(-1); P < 0.05). Despite a lower Vo(2max), Vo(2) kinetics is not different in older T2D compared with healthy age-matched control participants. The elevated Δ[HHb]/ΔVo(2) in T2D individuals possibly indicates a compromised muscle blood flow that mandates a greater O(2) extraction during exercise. Longer disease duration may result in adaptations in the O(2) extraction capabilities of individuals with T2D, thereby mitigating the expected age-related slowing of Vo(2) kinetics.
- Published
- 2011
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23. Influence of N-acetylcysteine administration on pulmonary O₂ uptake kinetics and exercise tolerance in humans.
- Author
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Bailey SJ, Winyard PG, Blackwell JR, Vanhatalo A, Lansley KE, Dimenna FJ, Wilkerson DP, Campbell IT, and Jones AM
- Subjects
- Adult, Analysis of Variance, Biomechanical Phenomena, Exercise Test, Humans, Male, Nitric Oxide blood, Plasma Volume drug effects, Plasma Volume physiology, Pulmonary Ventilation drug effects, Pulmonary Ventilation physiology, Time Factors, Young Adult, Acetylcysteine administration & dosage, Exercise Tolerance drug effects, Free Radical Scavengers administration & dosage, Oxygen Consumption drug effects, Pulmonary Gas Exchange physiology
- Abstract
We investigated the influence of the antioxidant N-acetylcysteine (NAC) on plasma nitrite concentration ([NO₂⁻]), pulmonary oxygen uptake (V(O₂)) kinetics and exercise tolerance. Eight males completed 'step' moderate- and severe-intensity cycle exercise tests following infusion of either NAC (125 mg kg⁻¹ h⁻¹ for 15 min followed by 25 mg kg⁻¹ h⁻¹ until the termination of exercise) or Placebo (PLA; saline). Following the initial loading phase, NAC infusion elevated plasma free sulfhydryl groups compared to placebo (PLA: 4 ± 2 vs. NAC: 13 ± 3 μ M g⁻¹; P < 0.05) and this elevation was preserved throughout the protocol. The administration of NAC did not significantly influence plasma [NO₂⁻] or V(O₂) kinetics during either moderate- or severe-intensity exercise. Although NAC did not significantly alter severe-intensity exercise tolerance at the group mean level (PLA: 776 ± 181 vs. NAC: 878 ± 284 s; P > 0.05), there was appreciable inter-subject variability in the response: four subjects had small reductions in exercise tolerance with NAC compared to PLA (-4%, -8%, -11%, and -14%) while the other four showed substantial improvements (+24%, +24%, +40%, and +69%). The results suggest that exercise-induced redox perturbations may contribute to fatigue development in recreationally-active adults., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2011
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24. Acute L-arginine supplementation reduces the O2 cost of moderate-intensity exercise and enhances high-intensity exercise tolerance.
- Author
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Bailey SJ, Winyard PG, Vanhatalo A, Blackwell JR, DiMenna FJ, Wilkerson DP, and Jones AM
- Subjects
- Administration, Oral, Adult, Arginine metabolism, Beverages, Blood Pressure, Cross-Over Studies, Double-Blind Method, England, Humans, Male, Muscle, Skeletal metabolism, Nitric Oxide metabolism, Nitrites blood, Pulmonary Gas Exchange, Time Factors, Young Adult, Arginine administration & dosage, Dietary Supplements, Exercise, Exercise Tolerance drug effects, Muscle Contraction drug effects, Muscle, Skeletal drug effects, Oxygen Consumption drug effects
- Abstract
It has recently been reported that dietary nitrate (NO(3)(-)) supplementation, which increases plasma nitrite (NO(2)(-)) concentration, a biomarker of nitric oxide (NO) availability, improves exercise efficiency and exercise tolerance in healthy humans. We hypothesized that dietary supplementation with L-arginine, the substrate for NO synthase (NOS), would elicit similar responses. In a double-blind, crossover study, nine healthy men (aged 19-38 yr) consumed 500 ml of a beverage containing 6 g of l-arginine (Arg) or a placebo beverage (PL) and completed a series of "step" moderate- and severe-intensity exercise bouts 1 h after ingestion of the beverage. Plasma NO(2)(-) concentration was significantly greater in the Arg than the PL group (331 ± 198 vs. 159 ± 102 nM, P < 0.05) and systolic blood pressure was significantly reduced (123 ± 3 vs. 131 ± 5 mmHg, P < 0.01). The steady-state O(2) uptake (VO(2)) during moderate-intensity exercise was reduced by 7% in the Arg group (1.48 ± 0.12 vs. 1.59 ± 0.14 l/min, P < 0.05). During severe-intensity exercise, the Vo(2) slow component amplitude was reduced (0.58 ± 0.23 and 0.76 ± 0.29 l/min in Arg and PL, respectively, P < 0.05) and the time to exhaustion was extended (707 ± 232 and 562 ± 145 s in Arg and PL, respectively, P < 0.05) following consumption of Arg. In conclusion, similar to the effects of increased dietary NO(3)(-) intake, elevating NO bioavailability through dietary L-Arg supplementation reduced the O(2) cost of moderate-intensity exercise and blunted the VO(2) slow component and extended the time to exhaustion during severe-intensity exercise.
- Published
- 2010
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25. Acute and chronic effects of dietary nitrate supplementation on blood pressure and the physiological responses to moderate-intensity and incremental exercise.
- Author
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Vanhatalo A, Bailey SJ, Blackwell JR, DiMenna FJ, Pavey TG, Wilkerson DP, Benjamin N, Winyard PG, and Jones AM
- Subjects
- Adult, Anaerobic Threshold drug effects, Anaerobic Threshold physiology, Cross-Over Studies, Dietary Supplements, Exercise Test, Female, Humans, Lactic Acid blood, Male, Nitric Oxide blood, Oxygen Consumption drug effects, Oxygen Consumption physiology, Pulmonary Gas Exchange physiology, Respiratory Mechanics drug effects, Respiratory Mechanics physiology, Beta vulgaris chemistry, Blood Pressure drug effects, Blood Pressure physiology, Exercise physiology, Nitrates pharmacology, Physical Fitness physiology
- Abstract
Dietary nitrate (NO(3)(-)) supplementation with beetroot juice (BR) over 4-6 days has been shown to reduce the O(2) cost of submaximal exercise and to improve exercise tolerance. However, it is not known whether shorter (or longer) periods of supplementation have similar (or greater) effects. We therefore investigated the effects of acute and chronic NO(3)(-) supplementation on resting blood pressure (BP) and the physiological responses to moderate-intensity exercise and ramp incremental cycle exercise in eight healthy subjects. Following baseline tests, the subjects were assigned in a balanced crossover design to receive BR (0.5 l/day; 5.2 mmol of NO(3)(-)/day) and placebo (PL; 0.5 l/day low-calorie juice cordial) treatments. The exercise protocol (two moderate-intensity step tests followed by a ramp test) was repeated 2.5 h following first ingestion (0.5 liter) and after 5 and 15 days of BR and PL. Plasma nitrite concentration (baseline: 454 ± 81 nM) was significantly elevated (+39% at 2.5 h postingestion; +25% at 5 days; +46% at 15 days; P < 0.05) and systolic and diastolic BP (baseline: 127 ± 6 and 72 ± 5 mmHg, respectively) were reduced by ∼4% throughout the BR supplementation period (P < 0.05). Compared with PL, the steady-state Vo(2) during moderate exercise was reduced by ∼4% after 2.5 h and remained similarly reduced after 5 and 15 days of BR (P < 0.05). The ramp test peak power and the work rate at the gas exchange threshold (baseline: 322 ± 67 W and 89 ± 15 W, respectively) were elevated after 15 days of BR (331 ± 68 W and 105 ± 28 W; P < 0.05) but not PL (323 ± 68 W and 84 ± 18 W). These results indicate that dietary NO(3)(-) supplementation acutely reduces BP and the O(2) cost of submaximal exercise and that these effects are maintained for at least 15 days if supplementation is continued.
- Published
- 2010
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26. Inspiratory muscle training enhances pulmonary O(2) uptake kinetics and high-intensity exercise tolerance in humans.
- Author
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Bailey SJ, Romer LM, Kelly J, Wilkerson DP, DiMenna FJ, and Jones AM
- Subjects
- Adaptation, Physiological, Adult, Biomarkers blood, Dyspnea physiopathology, Exercise Test, Female, Heart Rate, Humans, Kinetics, Lactic Acid blood, Male, Muscle Fatigue, Muscle, Skeletal blood supply, Oxygen Consumption, Regional Blood Flow, Young Adult, Breathing Exercises, Exercise Tolerance, Inhalation, Muscle Contraction, Muscle, Skeletal physiology, Oxygen metabolism, Pulmonary Gas Exchange, Respiratory Muscles physiology
- Abstract
Fatigue of the respiratory muscles during intense exercise might compromise leg blood flow, thereby constraining oxygen uptake (Vo(2)) and limiting exercise tolerance. We tested the hypothesis that inspiratory muscle training (IMT) would reduce inspiratory muscle fatigue, speed Vo(2) kinetics and enhance exercise tolerance. Sixteen recreationally active subjects (mean + or - SD, age 22 + or - 4 yr) were randomly assigned to receive 4 wk of either pressure threshold IMT [30 breaths twice daily at approximately 50% of maximum inspiratory pressure (MIP)] or sham treatment (60 breaths once daily at approximately 15% of MIP). The subjects completed moderate-, severe- and maximal-intensity "step" exercise transitions on a cycle ergometer before (Pre) and after (Post) the 4-wk intervention period for determination of Vo(2) kinetics and exercise tolerance. There were no significant changes in the physiological variables of interest after Sham. After IMT, baseline MIP was significantly increased (Pre vs. Post: 155 + or - 22 vs. 181 + or - 21 cmH(2)O; P < 0.001), and the degree of inspiratory muscle fatigue was reduced after severe- and maximal-intensity exercise. During severe exercise, the Vo(2) slow component was reduced (Pre vs. Post: 0.60 + or - 0.20 vs. 0.53 + or - 0.24 l/min; P < 0.05) and exercise tolerance was enhanced (Pre vs. Post: 765 + or - 249 vs. 1,061 + or - 304 s; P < 0.01). Similarly, during maximal exercise, the Vo(2) slow component was reduced (Pre vs. Post: 0.28 + or - 0.14 vs. 0.18 + or - 0.07 l/min; P < 0.05) and exercise tolerance was enhanced (Pre vs. Post: 177 + or - 24 vs. 208 + or - 37 s; P < 0.01). Four weeks of IMT, which reduced inspiratory muscle fatigue, resulted in a reduced Vo(2) slow-component amplitude and an improved exercise tolerance during severe- and maximal-intensity exercise. The results indicate that the enhanced exercise tolerance observed after IMT might be related, at least in part, to improved Vo(2) dynamics, presumably as a consequence of increased blood flow to the exercising limbs.
- Published
- 2010
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27. Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans.
- Author
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Bailey SJ, Fulford J, Vanhatalo A, Winyard PG, Blackwell JR, DiMenna FJ, Wilkerson DP, Benjamin N, and Jones AM
- Subjects
- Adult, Beta vulgaris, Beverages, Humans, Male, Muscle Contraction drug effects, Muscle Contraction physiology, Muscle, Skeletal chemistry, Nitrites analysis, Oxygen Consumption drug effects, Phosphocreatine analysis, Pulmonary Ventilation drug effects, Young Adult, Dietary Supplements, Exercise physiology, Knee physiology, Muscle Strength drug effects, Muscle, Skeletal drug effects, Nitrates administration & dosage
- Abstract
The purpose of this study was to elucidate the mechanistic bases for the reported reduction in the O(2) cost of exercise following short-term dietary nitrate (NO(3)(-)) supplementation. In a randomized, double-blind, crossover study, seven men (aged 19-38 yr) consumed 500 ml/day of either nitrate-rich beet root juice (BR, 5.1 mmol of NO(3)(-)/day) or placebo (PL, with negligible nitrate content) for 6 consecutive days, and completed a series of low-intensity and high-intensity "step" exercise tests on the last 3 days for the determination of the muscle metabolic (using (31)P-MRS) and pulmonary oxygen uptake (Vo(2)) responses to exercise. On days 4-6, BR resulted in a significant increase in plasma [nitrite] (mean +/- SE, PL 231 +/- 76 vs. BR 547 +/- 55 nM; P < 0.05). During low-intensity exercise, BR attenuated the reduction in muscle phosphocreatine concentration ([PCr]; PL 8.1 +/- 1.2 vs. BR 5.2 +/- 0.8 mM; P < 0.05) and the increase in Vo(2) (PL 484 +/- 41 vs. BR 362 +/- 30 ml/min; P < 0.05). During high-intensity exercise, BR reduced the amplitudes of the [PCr] (PL 3.9 +/- 1.1 vs. BR 1.6 +/- 0.7 mM; P < 0.05) and Vo(2) (PL 209 +/- 30 vs. BR 100 +/- 26 ml/min; P < 0.05) slow components and improved time to exhaustion (PL 586 +/- 80 vs. BR 734 +/- 109 s; P < 0.01). The total ATP turnover rate was estimated to be less for both low-intensity (PL 296 +/- 58 vs. BR 192 +/- 38 microM/s; P < 0.05) and high-intensity (PL 607 +/- 65 vs. BR 436 +/- 43 microM/s; P < 0.05) exercise. Thus the reduced O(2) cost of exercise following dietary NO(3)(-) supplementation appears to be due to a reduced ATP cost of muscle force production. The reduced muscle metabolic perturbation with NO(3)(-) supplementation allowed high-intensity exercise to be tolerated for a greater period of time.
- Published
- 2010
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28. Influence of priming exercise on muscle [PCr] and pulmonary O2 uptake dynamics during 'work-to-work' knee-extension exercise.
- Author
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Dimenna FJ, Fulford J, Bailey SJ, Vanhatalo A, Wilkerson DP, and Jones AM
- Subjects
- Adult, Electromyography, Humans, Magnetic Resonance Spectroscopy methods, Male, Nonlinear Dynamics, Pulmonary Gas Exchange physiology, Quadriceps Muscle metabolism, Young Adult, Exercise physiology, Knee Joint physiology, Muscle, Skeletal metabolism, Oxygen Consumption physiology, Physical Endurance physiology
- Abstract
Metabolic transitions from rest to high-intensity exercise were divided into two discrete steps (i.e., rest-to-moderate-intensity (R-->M) and moderate-to-high-intensity (M-->H)) to explore the effect of prior high-intensity 'priming' exercise on intramuscular [PCr] and pulmonary VO₂ kinetics for different sections of the motor unit pool. It was hypothesized that [PCr] and VO₂ kinetics would be unaffected by priming during R-->M exercise, but that the time constants (tau) describing the fundamental [PCr] response and the phase II VO₂ response would be significantly reduced by priming for M-->H exercise. On three separate occasions, six male subjects completed two identical R-->M/M-->H 'work-to-work' prone knee-extension exercise bouts separated by 5min rest. Two trials were performed with measurement of pulmonary VO₂ and the integrated electromyogram (iEMG) of the right m. vastus lateralis. The third trial was performed within the bore of a 1.5-T superconducting magnet for (31)P-MRS assessment of muscle metabolic responses. Priming did not significantly affect the [PCr] or VO₂ tau during R-->M ([PCr] tau Unprimed: 24+/-16 vs. Primed: 22+/-14s; VO₂ tau Unprimed: 26+/-8 vs. Primed: 25+/-9s) or M-->H transitions ([PCr] tau Unprimed: 30+/-5 vs. Primed: 32+/-7s; VO₂ tau Unprimed: 37+/-5 vs. Primed: 38+/-9s). However, it did reduce the amplitudes of the [PCr] and VO₂ slow components by 50% and 46%, respectively, during M-->H (P<0.05 for both comparisons). These effects were accompanied by iEMG changes suggesting reduced muscle fiber activation during M-->H exercise after priming. It is concluded that the tau for the initial exponential change of muscle [PCr] and pulmonary VO₂ following the transition from moderate-to-high-intensity prone knee-extension exercise is not altered by priming exercise., (Copyright 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
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29. Priming exercise speeds pulmonary O2 uptake kinetics during supine "work-to-work" high-intensity cycle exercise.
- Author
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DiMenna FJ, Wilkerson DP, Burnley M, Bailey SJ, and Jones AM
- Subjects
- Adult, Algorithms, Electromyography, Exercise Test, Heart Rate physiology, Hemoglobins metabolism, Humans, Kinetics, Lactic Acid blood, Leg physiology, Male, Pulmonary Gas Exchange physiology, Respiratory Mechanics physiology, Spectroscopy, Near-Infrared, Exercise physiology, Lung metabolism, Lung physiology, Oxygen Consumption physiology, Supine Position physiology
- Abstract
We manipulated the baseline metabolic rate and body position to explore the effect of the interaction between recruitment of discrete sections of the muscle fiber pool and muscle O(2) delivery on pulmonary O(2) uptake (VO(2)) kinetics during cycle exercise. We hypothesized that phase II VO(2) kinetics (tau(p)) in the transition from moderate- to severe-intensity exercise would be significantly slower in the supine than upright position because of a compromise to muscle perfusion and that a priming bout of severe-intensity exercise would return tau(p) during supine exercise to tau(p) during upright exercise. Eight male subjects [35 +/- 13 (SD) yr] completed a series of "step" transitions to severe-intensity cycle exercise from an "unloaded" (20-W) baseline and a baseline of moderate-intensity exercise in the supine and upright body positions. tau(p) was not significantly different between supine and upright exercise during transitions from a 20-W baseline to moderate- or severe-intensity exercise but was significantly greater during moderate- to severe-intensity exercise in the supine position (54 +/- 19 vs. 38 +/- 10 s, P < 0.05). Priming significantly reduced tau(p) during moderate- to severe-intensity supine exercise (34 +/- 9 s), returning it to a value that was not significantly different from tau(p) in the upright position. This effect occurred in the absence of changes in estimated muscle fractional O(2) extraction (from the near-infrared spectroscopy-derived deoxygenated Hb concentration signal), such that the priming-induced facilitation of muscle blood flow matched increased O(2) utilization in the recruited fibers, resulting in a speeding of VO(2) kinetics. These findings suggest that, during supine cycling, priming speeds VO(2) kinetics by providing an increased driving pressure for O(2) diffusion in the higher-order (i.e., type II) fibers, which would be recruited in the transition from moderate- to severe-intensity exercise and are known to be especially sensitive to limitations in O(2) supply.
- Published
- 2010
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30. Optimizing the "priming" effect: influence of prior exercise intensity and recovery duration on O2 uptake kinetics and severe-intensity exercise tolerance.
- Author
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Bailey SJ, Vanhatalo A, Wilkerson DP, Dimenna FJ, and Jones AM
- Subjects
- Adolescent, Analysis of Variance, Electromyography, Exercise Test, Exercise Tolerance, Heart Rate physiology, Humans, Lactic Acid blood, Male, Models, Biological, Muscle Contraction physiology, Muscle, Skeletal physiology, Spectroscopy, Near-Infrared, Time Factors, Young Adult, Athletic Performance physiology, Exercise physiology, Oxygen Consumption physiology
- Abstract
It has been suggested that a prior bout of high-intensity exercise has the potential to enhance performance during subsequent high-intensity exercise by accelerating the O(2) uptake (Vo(2)) on-response. However, the optimal combination of prior exercise intensity and subsequent recovery duration required to elicit this effect is presently unclear. Eight male participants, aged 18-24 yr, completed step cycle ergometer exercise tests to 80% of the difference between the preestablished gas exchange threshold and maximal Vo(2) (i.e., 80%Delta) after no prior exercise (control) and after six different combinations of prior exercise intensity and recovery duration: 40%Delta with 3 min (40-3-80), 9 min (40-9-80), and 20 min (40-20-80) of recovery and 70%Delta with 3 min (70-3-80), 9 min (70-9-80), and 20 min (70-20-80) of recovery. Overall Vo(2) kinetics were accelerated relative to control in all conditions except for 40-9-80 and 40-20-80 conditions as a consequence of a reduction in the Vo(2) slow component amplitude; the phase II time constant was not significantly altered with any prior exercise/recovery combination. Exercise tolerance at 80%Delta was improved by 15% and 30% above control in the 70-9-80 and 70-20-80 conditions, respectively, but was impaired by 16% in the 70-3-80 condition. Prior exercise at 40%Delta did not significantly influence exercise tolerance regardless of the recovery duration. These data demonstrate that prior high-intensity exercise ( approximately 70%Delta) can enhance the tolerance to subsequent high-intensity exercise provided that it is coupled with adequate recovery duration (>or=9 min). This combination presumably optimizes the balance between preserving the effects of prior exercise on Vo(2) kinetics and providing sufficient time for muscle homeostasis (e.g., muscle phosphocreatine and H(+) concentrations) to be restored.
- Published
- 2009
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31. Influence of extreme pedal rates on pulmonary O(2) uptake kinetics during transitions to high-intensity exercise from an elevated baseline.
- Author
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Dimenna FJ, Wilkerson DP, Burnley M, Bailey SJ, and Jones AM
- Subjects
- Adult, Bicycling physiology, Biomechanical Phenomena, Electromyography methods, Exercise Test methods, Humans, Lactic Acid blood, Male, Muscle, Skeletal physiology, Respiratory Mechanics physiology, Young Adult, Oxygen Consumption physiology, Physical Endurance physiology, Physical Exertion physiology, Pulmonary Gas Exchange physiology
- Abstract
We used extreme pedal rates to investigate the influence of muscle fibre recruitment on pulmonary V(O)(2) kinetics during unloaded-to-moderate-intensity (U-->M), unloaded-to-high-intensity (U-->H), and moderate-intensity to high-intensity (M-->H) cycling transitions. Seven healthy men completed transitions to 60% of the difference between gas-exchange threshold and peak V(O)(2) from both an unloaded and a moderate-intensity (95% GET) baseline at cadences of 35 and 115rpm. Pulmonary gas exchange was measured breath-by-breath and iEMG of the m. vastus lateralis and m. gluteus maximus was measured during all tests. At 35rpm, the phase II time constant (tau(p)) values for U-->M, U-->H, and M-->H were 26+/-7, 31+/-7 and 36+/-8s with the value for M-->H being longer than for U-->M (P<0.05). At 115rpm, the tau(p) values for U-->M, U-->H, and M-->H were 29+/-8, 48+/-16 and 53+/-20s with the value for U-->M being shorter than for the other two conditions (P<0.05). The V(O)(2) slow component was similar at both cadences, but iEMG only increased beyond minute 2 during high-intensity cycling at 115rpm. These results demonstrate that V(O)(2) kinetics are influenced by an interaction of exercise intensity and pedal rate and are consistent with the notion that changes in muscle fibre recruitment are responsible for slower phase II V(O)(2) kinetics during high-intensity and work-to-work exercise transitions.
- Published
- 2009
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32. Dietary nitrate supplementation reduces the O2 cost of low-intensity exercise and enhances tolerance to high-intensity exercise in humans.
- Author
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Bailey SJ, Winyard P, Vanhatalo A, Blackwell JR, Dimenna FJ, Wilkerson DP, Tarr J, Benjamin N, and Jones AM
- Subjects
- Administration, Oral, Adult, Beverages, Bicycling, Blood Pressure drug effects, Cross-Over Studies, Double-Blind Method, Hemoglobins metabolism, Humans, Male, Muscle Fatigue drug effects, Muscle, Skeletal metabolism, Nitrites blood, Oxyhemoglobins metabolism, Plant Roots, Spectroscopy, Near-Infrared, Time Factors, Young Adult, Beta vulgaris, Dietary Supplements, Exercise, Exercise Tolerance drug effects, Muscle Contraction, Muscle, Skeletal drug effects, Nitrites administration & dosage, Oxygen Consumption drug effects, Plant Preparations administration & dosage
- Abstract
Pharmacological sodium nitrate supplementation has been reported to reduce the O2 cost of submaximal exercise in humans. In this study, we hypothesized that dietary supplementation with inorganic nitrate in the form of beetroot juice (BR) would reduce the O2 cost of submaximal exercise and enhance the tolerance to high-intensity exercise. In a double-blind, placebo (PL)-controlled, crossover study, eight men (aged 19-38 yr) consumed 500 ml/day of either BR (containing 11.2 +/- 0.6 mM of nitrate) or blackcurrant cordial (as a PL, with negligible nitrate content) for 6 consecutive days and completed a series of "step" moderate-intensity and severe-intensity exercise tests on the last 3 days. On days 4-6, plasma nitrite concentration was significantly greater following dietary nitrate supplementation compared with PL (BR: 273 +/- 44 vs. PL: 140 +/- 50 nM; P < 0.05), and systolic blood pressure was significantly reduced (BR: 124 +/- 2 vs. PL: 132 +/- 5 mmHg; P < 0.01). During moderate exercise, nitrate supplementation reduced muscle fractional O2 extraction (as estimated using near-infrared spectroscopy). The gain of the increase in pulmonary O2 uptake following the onset of moderate exercise was reduced by 19% in the BR condition (BR: 8.6 +/- 0.7 vs. PL: 10.8 +/- 1.6 ml.min(-1).W(-1); P < 0.05). During severe exercise, the O2 uptake slow component was reduced (BR: 0.57 +/- 0.20 vs. PL: 0.74 +/- 0.24 l/min; P < 0.05), and the time-to-exhaustion was extended (BR: 675 +/- 203 vs. PL: 583 +/- 145 s; P < 0.05). The reduced O2 cost of exercise following increased dietary nitrate intake has important implications for our understanding of the factors that regulate mitochondrial respiration and muscle contractile energetics in humans.
- Published
- 2009
- Full Text
- View/download PDF
33. Influence of dichloroacetate on pulmonary gas exchange and ventilation during incremental exercise in healthy humans.
- Author
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Wilkerson DP, Campbell IT, Blackwell JR, Berger NJ, and Jones AM
- Subjects
- Adult, Bicarbonates blood, Energy Metabolism, Exercise Tolerance, Heart Rate drug effects, Humans, Lactic Acid blood, Male, Oxygen Consumption drug effects, Physical Exertion drug effects, Young Adult, Dichloroacetic Acid pharmacology, Exercise physiology, Pulmonary Gas Exchange drug effects, Pulmonary Ventilation drug effects
- Abstract
We hypothesised that dichloroacetate (DCA) would reduce blood lactate accumulation, pulmonary carbon dioxide output (.V(CO2)) and ventilation (.V(E)) at sub-maximal work rates, and improve exercise tolerance during incremental exercise in healthy humans. Nine males (mean+/-SD, age 27+/-4 years) completed, in random order, two ramp incremental cycle ergometer tests to the limit of tolerance following the intravenous infusion of DCA (75 mg/kg body mass in 80 ml saline) or an equivalent volume of saline (as placebo). Relative to control, blood [lactate] was significantly reduced by DCA immediately before exercise (CON: 0.7+/-0.2 vs. DCA: 0.5+/-0.2mM; P<0.05) and throughout exercise until 630s (P<0.05). Blood [HCO(3)(-)] was significantly higher in the DCA condition from 360s until 720s of exercise (P<0.05). .V(CO2) and .V(E) were both lower throughout exercise in the DCA condition, with the differences reaching significance at 90 and 180s for .V(CO2) (P<0.05) and at 90, 180, 450, 540, 630, and 810s for .V(E) (P<0.05). Exercise tolerance was not significantly altered (CON: 1029+/-109 vs. DCA: 1045+/-101s). Infusion of DCA resulted in reductions in blood [lactate], .V(CO2) and .V(E) during sub-maximal incremental exercise, consistent with the existence of a link between the bicarbonate buffering of metabolic acidosis and increased CO(2) output. However, the reduced blood lactate accumulation during sub-maximal exercise with DCA did not enhance exercise tolerance.
- Published
- 2009
- Full Text
- View/download PDF
34. Influence of repeated sprint training on pulmonary O2 uptake and muscle deoxygenation kinetics in humans.
- Author
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Bailey SJ, Wilkerson DP, Dimenna FJ, and Jones AM
- Subjects
- Exercise Test, Female, Hemoglobins analysis, Hemoglobins metabolism, Humans, Male, Oxygen metabolism, Spectroscopy, Near-Infrared, Young Adult, Adaptation, Physiological physiology, Bicycling physiology, Exercise physiology, Lung metabolism, Muscle, Skeletal metabolism, Oxygen Consumption physiology
- Abstract
We hypothesized that a short-term training program involving repeated all-out sprint training (RST) would be more effective than work-matched, low-intensity endurance training (ET) in enhancing the kinetics of oxygen uptake (Vo(2)) and muscle deoxygenation {deoxyhemoglobin concentration ([HHb])} following the onset of exercise. Twenty-four recreationally active subjects (15 men, mean +/- SD: age 21 +/- 4 yr, height 173 +/- 9 cm, body mass 71 +/- 11 kg) were allocated to one of three groups: RST, which completed six sessions of four to seven 30-s RSTs; ET, which completed six sessions of work-matched, moderate-intensity cycling; and a control group (CON). All subjects completed moderate-intensity and severe-intensity "step" exercise transitions before (Pre) and after the 2-wk intervention period (Post). Following RST, [HHb] kinetics were speeded, and the amplitude of the [HHb] response was increased during both moderate and severe exercise (P < 0.05); the phase II Vo(2) kinetics were accelerated for both moderate (Pre: 28 +/- 8, Post: 21 +/- 8 s; P < 0.01) and severe (Pre: 29 +/- 5, Post: 23 +/- 5 s; P < 0.05) exercise; the amplitude of the Vo(2) slow component was reduced (Pre: 0.52 +/- 0.19, Post: 0.40 +/- 0.17 l/min; P < 0.01); and exercise tolerance during severe exercise was improved by 53% (Pre: 700 +/- 234, Post: 1,074 +/- 431 s; P < 0.01). None of these parameters was significantly altered in the ET and CON groups. Six sessions of RST, but not ET, resulted in changes in [HHb] kinetics consistent with enhanced fractional muscle O(2) extraction, faster Vo(2) kinetics, and an increased tolerance to high-intensity exercise.
- Published
- 2009
- Full Text
- View/download PDF
35. Muscular and pulmonary O2 uptake kinetics during moderate- and high-intensity sub-maximal knee-extensor exercise in humans.
- Author
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Krustrup P, Jones AM, Wilkerson DP, Calbet JA, and Bangsbo J
- Subjects
- Adult, Anaerobic Threshold physiology, Capillaries physiology, Exercise Test, Humans, Kinetics, Knee physiology, Male, Models, Statistical, Muscle, Skeletal blood supply, Pulmonary Gas Exchange, Regional Blood Flow physiology, Supine Position physiology, Thigh blood supply, Young Adult, Exercise physiology, Lung metabolism, Muscle, Skeletal metabolism, Oxygen Consumption physiology
- Abstract
The purpose of this investigation was to determine the contribution of muscle O(2) consumption (mVO2) to pulmonary O(2) uptake (pVO2) during both low-intensity (LI) and high-intensity (HI) knee-extension exercise, and during subsequent recovery, in humans. Seven healthy male subjects (age 20-25 years) completed a series of LI and HI square-wave exercise tests in which mVO2 (direct Fick technique) and pVO2 (indirect calorimetry) were measured simultaneously. The mean blood transit time from the muscle capillaries to the lung (MTTc-l) was also estimated (based on measured blood transit times from femoral artery to vein and vein to artery). The kinetics of mVO2 and pVO2 were modelled using non-linear regression. The time constant (tau) describing the phase II pVO2 kinetics following the onset of exercise was not significantly different from the mean response time (initial time delay + tau) for mVO2 kinetics for LI (30 +/- 3 vs 30 +/- 3 s) but was slightly higher (P < 0.05) for HI (32 +/- 3 vs 29 +/- 4 s); the responses were closely correlated (r = 0.95 and r = 0.95; P < 0.01) for both intensities. In recovery, agreement between the responses was more limited both for LI (36 +/- 4 vs 18 +/- 4 s, P < 0.05; r = -0.01) and HI (33 +/- 3 vs 27 +/- 3 s, P > 0.05; r = -0.40). MTTc-l was approximately 17 s just before exercise and decreased to 12 and 10 s after 5 s of exercise for LI and HI, respectively. These data indicate that the phase II pVO2 kinetics reflect mVO2 kinetics during exercise but not during recovery where caution in data interpretation is advised. Increased mVO2 probably makes a small contribution to during the first 15-20 s of exercise.
- Published
- 2009
- Full Text
- View/download PDF
36. Influence of dietary creatine supplementation on muscle phosphocreatine kinetics during knee-extensor exercise in humans.
- Author
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Jones AM, Wilkerson DP, and Fulford J
- Subjects
- Adenosine Diphosphate metabolism, Adenosine Triphosphate metabolism, Adult, Creatine metabolism, Humans, Kinetics, Magnetic Resonance Spectroscopy, Male, Muscle Fatigue drug effects, Muscle, Skeletal metabolism, Oxidative Phosphorylation drug effects, Phosphorus Isotopes, Recovery of Function drug effects, Young Adult, Creatine administration & dosage, Dietary Supplements, Exercise, Muscle Contraction, Muscle, Skeletal drug effects, Phosphocreatine metabolism
- Abstract
We hypothesized that increasing skeletal muscle total creatine (Cr) content through dietary Cr supplementation would result in slower muscle phosphocreatine concentration ([PCr]) kinetics, as assessed using (31)P magnetic resonance spectroscopy, following the onset and offset of both moderate-intensity (Mod) and heavy-intensity (Hvy) exercise. Seven healthy males (age 29 +/- 6 yr, mean +/- SD) completed a series of square-wave transitions to Mod and Hvy knee extensor exercise inside the bore of a 1.5-T superconducting magnet both before and after a 5-day period of Cr loading (4x 5 g/day of creatine monohydrate). Cr supplementation resulted in an approximately 8% increase in the resting muscle [PCr]-to-[ATP] ratio (4.66 +/- 0.27 vs. 5.04 +/- 0.22; P < 0.05), consistent with a significant increase in muscle total Cr content consequent to the intervention. The time constant for muscle [PCr] kinetics was increased following Cr loading for Mod exercise (control: 15 +/- 8 vs. Cr: 25 +/- 9 s; P < 0.05) and subsequent recovery (control: 14 +/- 8 vs. Cr: 27 +/- 8 s; P < 0.05) and for Hvy exercise (control: 54 +/- 18 vs. Cr: 72 +/- 30 s; P < 0.05), but not for subsequent recovery (control: 41 +/- 11 vs. Cr: 44 +/- 6 s). The magnitude of the increase in [PCr] following Cr loading was correlated (P < 0.05) with the extent of the slowing of the [PCr] kinetics for the moderate off-transient (r = 0.92) and the heavy on-transient (r = 0.71). These data demonstrate, for the first time in humans, that an increase in muscle [PCr] results in a slowing of [PCr] dynamics in exercise and subsequent recovery.
- Published
- 2009
- Full Text
- View/download PDF
37. Influence of priming exercise on pulmonary O2 uptake kinetics during transitions to high-intensity exercise at extreme pedal rates.
- Author
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DiMenna FJ, Wilkerson DP, Burnley M, Bailey SJ, and Jones AM
- Subjects
- Adult, Bicycling, Heart Rate, Hemoglobins metabolism, Humans, Kinetics, Male, Muscle Fibers, Fast-Twitch metabolism, Spectrophotometry, Infrared, Young Adult, Exercise, Muscle Contraction, Muscle, Skeletal metabolism, Oxygen metabolism, Oxygen Consumption, Pulmonary Ventilation
- Abstract
We investigated the pedal rate dependency of the effect of priming exercise on pulmonary oxygen uptake (Vo(2)) kinetics. Seven healthy men completed two, 6-min bouts of high-intensity cycle exercise (separated by 6 min of rest) using different combinations of extreme pedal rates for the priming and criterion exercise bouts (i.e., 35-->35, 35-->115, 115-->35, and 115-->115 rev/min). Pulmonary gas exchange and heart rate were measured breath-by-breath, and muscle oxygenation was assessed using near-infrared spectroscopy. When the priming bout was performed at 35 rev/min (35-->35 and 35-->115 conditions), the phase II Vo(2) time constant (tau) was not significantly altered (bout 1: 31 +/- 7 vs. bout 2: 30 +/- 5 s and bout 1: 48 +/- 16 vs. bout 2: 46 +/- 21 s, respectively). However, when the priming bout was performed at 115 rev/min (115-->35 and 115-->115 conditions), the phase II tau was significantly reduced (bout 1: 31 +/- 7 vs. bout 2: 26 +/- 5 s and bout 1: 48 +/- 16 vs. bout 2: 39 +/- 9 s, respectively, P < 0.05). Muscle oxygenation was significantly higher after priming exercise in all four conditions, but significant effects on Vo(2) kinetics were only evident when muscle O(2) extraction (measured as Delta[deoxyhemoglobin]/DeltaVo(2)) was elevated in the fundamental response phase. These data indicate that prior high-intensity exercise at a high pedal rate can speed Vo(2) kinetics during subsequent high-intensity exercise, presumably through specific priming effects on type II muscle fibers.
- Published
- 2009
- Full Text
- View/download PDF
38. Effect of eccentric exercise-induced muscle damage on the dynamics of muscle oxygenation and pulmonary oxygen uptake.
- Author
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Davies RC, Eston RG, Poole DC, Rowlands AV, DiMenna F, Wilkerson DP, Twist C, and Jones AM
- Subjects
- Adaptation, Physiological, Adult, Biomarkers blood, Creatine Kinase blood, Hemoglobins metabolism, Humans, Kinetics, Lung blood supply, Male, Microcirculation, Muscle, Skeletal blood supply, Muscle, Skeletal pathology, Muscular Diseases pathology, Pulmonary Circulation, Spectroscopy, Near-Infrared, Young Adult, Exercise, Lung metabolism, Muscle, Skeletal metabolism, Muscular Diseases metabolism, Oxygen blood, Oxygen Consumption
- Abstract
Unaccustomed eccentric exercise has a profound impact on muscle structure and function. However, it is not known whether associated microvascular dysfunction disrupts the matching of O2 delivery (Qo2) to O2 utilization (Vo2). Near-infrared spectroscopy (NIRS) was used to test the hypothesis that eccentric exercise-induced muscle damage would elevate the muscle Qo2:Vo2 ratio during severe-intensity exercise while preserving the speed of the Vo2 kinetics at exercise onset. Nine physically active men completed "step" tests to severe-intensity exercise from an unloaded baseline on a cycle ergometer before (Pre) and 48 h after (Post) eccentric exercise (100 squats with a load corresponding to 70% of body mass). NIRS and breath-by-breath pulmonary Vo2 were measured continuously during the exercise tests and subsequently modeled using standard nonlinear regression techniques. There were no changes in phase II pulmonary Vo2 kinetics following the onset of exercise (time constant: Pre, 25 +/- 4 s; Post, 24 +/- 2 s; amplitude: Pre, 2.36 +/- 0.23 l/min; Post, 2.37 +/- 0.23 l/min; all P > 0.05). However, the primary (Pre, 14 +/- 3 s; Post, 19 +/- 3 s) and overall (Pre, 16 +/- 4 s; Post, 21 +/- 4 s) mean response time of the [HHb] response was significantly slower following eccentric exercise (P < 0.05). The slower [HHb] kinetics observed following eccentric exercise is consistent with an increased Qo2:Vo2 ratio during transitions to severe-intensity exercise. We propose that unchanged primary phase Vo2 kinetics are associated with an elevated Qo2:Vo2 ratio that preserves blood-myocyte O2 flux.
- Published
- 2008
- Full Text
- View/download PDF
39. Influence of priming exercise on pulmonary O2 uptake kinetics during transitions to high-intensity exercise from an elevated baseline.
- Author
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DiMenna FJ, Wilkerson DP, Burnley M, and Jones AM
- Subjects
- Adult, Anaerobiosis, Bicycling physiology, Data Interpretation, Statistical, Electromyography, Heart Rate physiology, Humans, Kinetics, Lactic Acid blood, Male, Muscle, Skeletal innervation, Muscle, Skeletal physiology, Pulmonary Gas Exchange physiology, Respiratory Mechanics physiology, Exercise physiology, Lung metabolism, Oxygen Consumption physiology
- Abstract
It has been suggested that the slower O2 uptake (VO2) kinetics observed when exercise is initiated from an elevated baseline metabolic rate are linked to an impairment of muscle O2 delivery. We hypothesized that "priming" exercise would significantly reduce the phase II time constant (tau) during subsequent severe-intensity cycle exercise initiated from an elevated baseline metabolic rate. Seven healthy men completed exercise transitions to 70% of the difference between gas exchange threshold (GET) and peak VO2 from a moderate-intensity baseline (90% GET) on three occasions in each of the "unprimed" and "primed" conditions. Pulmonary gas exchange, heart rate, and the electromyogram of m. vastus lateralis were measured during all tests. The phase II VO2 kinetics were slower when severe exercise was initiated from a baseline of moderate exercise compared with unloaded pedaling (mean+/-SD tau, 42+/-15 vs. 33+/-8 s; P<0.05), but were not accelerated by priming exercise (42+/-17 s; P>0.05). The amplitude of the VO2 slow component and the change in electromyogram from minutes 2 to 6 were both significantly reduced following priming exercise (VO2 slow component: from 0.47+/-0.09 to 0.27+/-0.13 l/min; change in integrated electromyogram between 2 and 6 min: from 51+/-35 to 26+/-43% of baseline; P<0.05 for both comparisons). These results indicate that the slower phase II VO2 kinetics observed during transitions to severe exercise from an elevated baseline are not altered by priming exercise, but that the reduced VO2 slow component may be linked to changes in muscle fiber activation.
- Published
- 2008
- Full Text
- View/download PDF
40. Influence of prior exercise on muscle [phosphorylcreatine] and deoxygenation kinetics during high-intensity exercise in men.
- Author
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Jones AM, Fulford J, and Wilkerson DP
- Subjects
- Adult, Humans, Hydrogen-Ion Concentration, Hydrolysis, Kinetics, Magnetic Resonance Spectroscopy, Male, Oxygen blood, Oxyhemoglobins metabolism, Spectroscopy, Near-Infrared, Exercise physiology, Muscle Contraction, Muscle Fatigue, Oxygen metabolism, Oxygen Consumption, Phosphocreatine metabolism, Quadriceps Muscle metabolism
- Abstract
(31)Phosphate-magnetic resonance spectroscopy and near infrared spectroscopy (NIRS) were used for the simultaneous assessment of changes in quadriceps muscle metabolism and oxygenation during consecutive bouts of high-intensity exercise. Six male subjects completed two 6 min bouts of single-legged knee-extension exercise at 80% of the peak work rate separated by 6 min of rest while positioned inside the bore of a 1.5 T superconducting magnet. The total haemoglobin and oxyhaemoglobin concentrations in the area of the quadriceps muscle interrogated with NIRS were significantly higher in the baseline period prior to the second compared with the first exercise bout, consistent with an enhanced muscle oxygenation. Intramuscular phosphorylcreatine concentration ([PCr]) dynamics were not different over the fundamental region of the response (time constant for bout 1, 51 +/- 15 s versus bout 2, 52 +/- 17 s). However, the [PCr] dynamics over the entire response were faster in the second bout (mean response time for bout 1, 72 +/- 16 s versus bout 2, 57 +/- 8 s; P < 0.05), as a consequence of a greater fall in [PCr] in the fundamental phase and a reduction in the magnitude of the 'slow component' in [PCr] beyond 3 min of exercise (bout 1, 10 +/- 6% versus bout 2, 5 +/- 3%; P < 0.05). These data suggest that the increased muscle O(2) availability afforded by the performance of a prior bout of high-intensity exercise does not significantly alter the kinetics of [PCr] hydrolysis at the onset of a subsequent bout of high-intensity exercise. The greater fall in [PCr] over the fundamental phase of the response following prior high-intensity exercise indicates that residual fatigue acutely reduces muscle efficiency.
- Published
- 2008
- Full Text
- View/download PDF
41. Validity of criteria for establishing maximal O2 uptake during ramp exercise tests.
- Author
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Poole DC, Wilkerson DP, and Jones AM
- Subjects
- Adult, Heart Rate physiology, Humans, Lactic Acid blood, Male, Pulmonary Gas Exchange physiology, Reproducibility of Results, Exercise Test methods, Exercise Test standards, Oxygen Consumption physiology, Physical Exertion physiology
- Abstract
The incremental or ramp exercise test to the limit of tolerance has become a popular test for determination of maximal O(2) uptake (VO(2max)). However, many subjects do not evidence a definitive plateau of the VO(2) -work rate relationship on this test and secondary criteria based upon respiratory exchange ratio (RER), maximal heart rate (HR(max)) or blood [lactate] have been adopted to provide confidence in the measured VO(2max). We hypothesized that verification of VO(2max) using these variables is fundamentally flawed in that their use could either allow underestimation of VO(2max) (if, for any reason, a test were ended at a sub-maximal [Formula: see text]), or alternatively preclude subjects from recording a valid VO(2max). Eight healthy male subjects completed a ramp exercise test (at 20 W/min) to the limit of tolerance on an electrically braked cycle ergometer during which pulmonary gas exchange was measured breath-by-breath and blood [lactate] was determined every 90 s. Using the most widely used criterion values of RER (1.10 and 1.15), VO(2max) as determined during the ramp test (4.03 +/- 0.10 l/min) could be undermeasured by 27% (2.97 +/- 0.24 l/min) and 16% (3.41 +/- 0.15 l/min), respectively (both P < 0.05). The criteria of HR(max) (age predicted HR(max) +/- 10 b/min) and blood [lactate] (> or = 8 mM) were untenable because they resulted in rejection of 3/8 and 6/8 of the subjects, most of whom (5/8) had demonstrated a plateau of VO(2max) at VO(2max). These findings provide a clear mandate for rejecting these secondary criteria as a means of validating VO(2max) on ramp exercise tests.
- Published
- 2008
- Full Text
- View/download PDF
42. Muscle metabolic responses to exercise above and below the "critical power" assessed using 31P-MRS.
- Author
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Jones AM, Wilkerson DP, DiMenna F, Fulford J, and Poole DC
- Subjects
- Adult, Exercise physiology, Humans, Male, Oxygen Consumption physiology, Phosphorus Isotopes, Energy Metabolism physiology, Magnetic Resonance Spectroscopy, Muscle Fatigue physiology, Muscle, Skeletal metabolism, Physical Endurance physiology
- Abstract
We tested the hypothesis that the asymptote of the hyperbolic relationship between work rate and time to exhaustion during muscular exercise, the "critical power" (CP), represents the highest constant work rate that can be sustained without a progressive loss of homeostasis [as assessed using (31)P magnetic resonance spectroscopy (MRS) measurements of muscle metabolites]. Six healthy male subjects initially completed single-leg knee-extension exercise at three to four different constant work rates to the limit of tolerance (range 3-18 min) for estimation of the CP (mean +/- SD, 20 +/- 2 W). Subsequently, the subjects exercised at work rates 10% below CP (
CP) for as long as possible, while the metabolic responses in the contracting quadriceps muscle, i.e., phosphorylcreatine concentration ([PCr]), P(i) concentration ([P(i)]), and pH, were estimated using (31)P-MRS. All subjects completed 20 min of CP exercise was 14.7 +/- 7.1 min. During CP exercise, however, [PCr] continued to fall to the point of exhaustion and [P(i)] and pH changed precipitously to values that are typically observed at the termination of high-intensity exhaustive exercise (end-exercise values = 26 +/- 16% of baseline [PCr], 564 +/- 167% of baseline [P(i)], and pH 6.87 +/- 0.10, all P < 0.05 vs. - Published
- 2008
- Full Text
- View/download PDF
43. Muscle [phosphocreatine] dynamics following the onset of exercise in humans: the influence of baseline work-rate.
- Author
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Jones AM, Wilkerson DP, and Fulford J
- Subjects
- Adenosine Triphosphate metabolism, Adult, Cell Respiration physiology, Ergometry, Humans, Magnetic Resonance Spectroscopy, Male, Models, Biological, Oxidative Phosphorylation, Exercise physiology, Muscle, Skeletal metabolism, Phosphocreatine metabolism, Physical Exertion physiology
- Abstract
The kinetics of pulmonary O(2) uptake is known to be substantially slower when exercise is initiated from a baseline of lower-intensity exercise rather than from rest. However, it is not known whether putative intracellular regulators of mitochondrial respiration (and in particular the phosphocreatine concentration, [PCr]) show similar non-linearities in their response dynamics. The purpose of this study was therefore to investigate the influence of baseline metabolic rate on muscle [PCr] kinetics (as assessed using (31)P-magnetic resonance spectroscopy) following the onset of exercise. Seven male subjects completed 'step' tests to heavy-intensity exercise (80% of peak work-rate) from a resting baseline and also from a baseline of moderate-intensity exercise (40% of peak work-rate) using a single-leg knee-extensor ergometer situated inside the bore of a 1.5 T super-conducting magnet. The time constant describing the kinetics of the initial exponential-like fall in [PCr] was significantly different between rest-to-moderate (25 +/- 14 s), rest-to-heavy (48 +/- 11 s) and moderate-to-heavy exercise (95 +/- 40 s) (P < 0.05 for all comparisons). A delayed-onset 'slow component' in the [PCr] response was observed in all subjects during rest-to-heavy exercise, but was attenuated in the moderate-to-heavy exercise condition. These data indicate that muscle [PCr] kinetics does not conform to 'linear, first-order' behaviour during dynamic exercise, and thus have implications for understanding the regulation of muscle oxidative metabolism.
- Published
- 2008
- Full Text
- View/download PDF
44. Influence of endurance training on muscle [PCr] kinetics during high-intensity exercise.
- Author
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Jones AM, Wilkerson DP, Berger NJ, and Fulford J
- Subjects
- Adult, Algorithms, Bicycling, Ergometry, Humans, Hydrogen-Ion Concentration, Kinetics, Magnetic Resonance Spectroscopy, Male, Models, Statistical, Muscle, Skeletal chemistry, Exercise physiology, Muscle, Skeletal metabolism, Phosphocreatine metabolism, Physical Endurance physiology, Physical Fitness physiology
- Abstract
We hypothesized that a period of endurance training would result in a speeding of muscle phosphocreatine concentration ([PCr]) kinetics over the fundamental phase of the response and a reduction in the amplitude of the [PCr] slow component during high-intensity exercise. Six male subjects (age 26 +/- 5 yr) completed 5 wk of single-legged knee-extension exercise training with the alternate leg serving as a control. Before and after the intervention period, the subjects completed incremental and high-intensity step exercise tests of 6-min duration with both legs separately inside the bore of a whole-body magnetic resonance spectrometer. The time-to-exhaustion during incremental exercise was not changed in the control leg [preintervention group (PRE): 19.4 +/- 2.3 min vs. postintervention group (POST): 19.4 +/- 1.9 min] but was significantly increased in the trained leg (PRE: 19.6 +/- 1.6 min vs. POST: 22.0 +/- 2.2 min; P < 0.05). During step exercise, there were no significant changes in the control leg, but end-exercise pH and [PCr] were higher after vs. before training. The time constant for the [PCr] kinetics over the fundamental exponential region of the response was not significantly altered in either the control leg (PRE: 40 +/- 13 s vs. POST: 43 +/- 10 s) or the trained leg (PRE: 38 +/- 8 s vs. POST: 40 +/- 12 s). However, the amplitude of the [PCr] slow component was significantly reduced in the trained leg (PRE: 15 +/- 7 vs. POST: 7 +/- 7% change in [PCr]; P < 0.05) with there being no change in the control leg (PRE: 13 +/- 8 vs. POST: 12 +/- 10% change in [PCr]). The attenuation of the [PCr] slow component might be mechanistically linked with enhanced exercise tolerance following endurance training.
- Published
- 2007
- Full Text
- View/download PDF
45. Effects of baseline metabolic rate on pulmonary O2 uptake on-kinetics during heavy-intensity exercise in humans.
- Author
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Wilkerson DP and Jones AM
- Subjects
- Adult, Exercise Test, Humans, Male, Muscle, Skeletal metabolism, Reference Values, Basal Metabolism physiology, Exercise physiology, Oxygen Consumption physiology, Physical Exertion physiology, Pulmonary Gas Exchange physiology
- Abstract
We hypothesised that initiating heavy-intensity exercise from an elevated baseline metabolic rate would result in slower Phase II O2 uptake V(O2) kinetics and a greater overall 'gain' in V(O2) per unit increase in work rate. Seven healthy males performed a series of like-transitions on a cycle ergometer: (1) from light exercise to 'moderate' exercise (80% of the gas exchange threshold, GET; L-->M); (2) from light exercise to 'heavy' exercise (40% of the difference between GET and V(O2) peak; L-->H); (3) from moderate exercise to heavy exercise (M-->H). The Phase II time constant (tau) was significantly (P<0.01) greater in the M-->H condition (48+/-11 s) compared to the L-->M and L-->H conditions (26+/-6 s versus 27+/-4 s, respectively). Moreover, the end-exercise 'gain' values were significantly different between the three conditions (L-->M, 8.1+/-0.7 mL min-1 W-1; L-->H, 9.7+/-0.4 mL min-1 W-1; M-->H, 10.7+/-0.7 mL min-1 W-1; P<0.05). This 'non-linearity' in the pulmonary V(O2) response to exercise might be attributed, at least in part, to differences in the metabolic properties of the muscle fibres recruited in the abrupt transition from a lower to a higher work rate.
- Published
- 2007
- Full Text
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46. Effects of "priming" exercise on pulmonary O2 uptake and muscle deoxygenation kinetics during heavy-intensity cycle exercise in the supine and upright positions.
- Author
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Jones AM, Berger NJ, Wilkerson DP, and Roberts CL
- Subjects
- Adult, Exercise Test, Humans, Hyperemia metabolism, Lactic Acid blood, Lung metabolism, Male, Muscle, Skeletal metabolism, Oxygen analysis, Oxygen metabolism, Spectroscopy, Near-Infrared, Adaptation, Physiological physiology, Energy Metabolism physiology, Exercise physiology, Oxygen Consumption physiology, Posture physiology, Pulmonary Gas Exchange physiology
- Abstract
We hypothesized that the performance of prior heavy exercise would speed the phase 2 oxygen consumption (VO2) kinetics during subsequent heavy exercise in the supine position (where perfusion pressure might limit muscle O2 supply) but not in the upright position. Eight healthy men (mean +/- SD age 24 +/- 7 yr; body mass 75.0 +/- 5.8 kg) completed a double-step test protocol involving two bouts of 6 min of heavy cycle exercise, separated by a 10-min recovery period, on two occasions in each of the upright and supine positions. Pulmonary O2 uptake was measured breath by breath and muscle oxygenation was assessed using near-infrared spectroscopy (NIRS). The NIRS data indicated that the performance of prior exercise resulted in hyperemia in both body positions. In the upright position, prior exercise had no significant effect on the time constant tau of the VO2 response in phase 2 (bout 1: 29 +/- 10 vs. bout 2: 28 +/- 4 s; P = 0.91) but reduced the amplitude of the VO2 slow component (bout 1: 0.45 +/- 0.16 vs. bout 2: 0.22 +/- 0.14 l/min; P = 0.006) during subsequent heavy exercise. In contrast, in the supine position, prior exercise resulted in a significant reduction in the phase 2 tau (bout 1: 38 +/- 18 vs. bout 2: 24 +/- 9 s; P = 0.03) but did not alter the amplitude of the VO2 slow component (bout 1: 0.40 +/- 0.29 vs. bout 2: 0.41 +/- 0.20 l/min; P = 0.86). These results suggest that the performance of prior heavy exercise enables a speeding of phase 2 VO2 kinetics during heavy exercise in the supine position, presumably by negating an O2 delivery limitation that was extant in the control condition, but not during upright exercise, where muscle O2 supply was probably not limiting.
- Published
- 2006
- Full Text
- View/download PDF
47. Influence of acute plasma volume expansion on VO2 kinetics, VO2 peak, and performance during high-intensity cycle exercise.
- Author
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Berger NJ, Campbell IT, Wilkerson DP, and Jones AM
- Subjects
- Adult, Exercise Test, Exercise Tolerance drug effects, Humans, Male, Metabolic Clearance Rate drug effects, Oxygen Consumption drug effects, Physical Exertion drug effects, Plasma Volume drug effects, Polygeline administration & dosage, Exercise Tolerance physiology, Oxygen metabolism, Oxygen Consumption physiology, Physical Exertion physiology, Plasma Substitutes administration & dosage, Plasma Volume physiology, Task Performance and Analysis
- Abstract
The purpose of this study was to examine the influence of acute plasma volume expansion (APVE) on oxygen uptake (V(O2)) kinetics, V(O2peak), and time to exhaustion during severe-intensity exercise. Eight recreationally active men performed "step" cycle ergometer exercise tests at a work rate requiring 70% of the difference between the gas-exchange threshold and V(O2max) on three occasions: twice as a "control" (Con) and once after intravenous infusion of a plasma volume expander (Gelofusine; 7 ml/kg body mass). Pulmonary gas exchange was measured breath by breath. APVE resulted in a significant reduction in hemoglobin concentration (preinfusion: 16.0 +/- 1.0 vs. postinfusion: 14.7 +/- 0.8 g/dl; P < 0.001) and hematocrit (preinfusion: 44 +/- 2 vs. postinfusion: 41 +/- 3%; P < 0.01). Despite this reduction in arterial O(2) content, APVE had no effect on V(O2) kinetics (phase II time constant, Con: 33 +/- 15 vs. APVE: 34 +/- 12 s; P = 0.74), and actually resulted in an increased V(O2peak) (Con: 3.90 +/- 0.56 vs. APVE: 4.12 +/- 0.55 l/min; P = 0.006) and time to exhaustion (Con: 365 +/- 58 vs. APVE: 424 +/- 64 s; P = 0.04). The maximum O(2) pulse was also enhanced by the treatment (Con: 21.3 +/- 3.4 vs. APVE: 22.7 +/- 3.4 ml/beat; P = 0.04). In conclusion, APVE does not alter V(O2) kinetics but enhances V(O2peak) and exercise tolerance during high-intensity cycle exercise in young recreationally active subjects.
- Published
- 2006
- Full Text
- View/download PDF
48. Lactic acid accumulation is an advantage/disadvantage during muscle activity.
- Author
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Burnley M, Wilkerson DP, and Jones AM
- Subjects
- Humans, Lactic Acid analysis, Muscle Fatigue physiology, Muscle, Skeletal chemistry, Oxygen Consumption physiology, Exercise physiology, Lactic Acid blood, Muscle Contraction physiology, Muscle, Skeletal metabolism
- Published
- 2006
- Full Text
- View/download PDF
49. Influence of hyperoxia on pulmonary O2 uptake kinetics following the onset of exercise in humans.
- Author
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Wilkerson DP, Berger NJ, and Jones AM
- Subjects
- Adult, Exercise Test methods, Humans, Hyperoxia physiopathology, Male, Pulmonary Gas Exchange physiology, Time Factors, Exercise physiology, Hyperoxia rehabilitation, Inhalation physiology, Oxygen Consumption physiology
- Abstract
The purpose of this study was to examine the influence of hyperoxic gas (50% O2 in N2) inspiration on pulmonary oxygen uptake (V(O2)) kinetics during step transitions to moderate, severe and supra-maximal intensity cycle exercise. Seven healthy male subjects completed repeat transitions to moderate (90% of the gas exchange threshold, GET), severe (70% of the difference between the GET and V(O2) peak) and supra-maximal (105% V(O2) peak) intensity work rates while breathing either normoxic (N) or hyperoxic (H) gas before and during exercise. Hyperoxia had no significant effect on the Phase II V(O2) time constant during moderate (N: 28+/-3s versus H: 31+/-7s), severe (N: 32+/-9s versus H: 33+/-6s) or supra-maximal (N: 37+/-9s versus H: 37+/-9s) exercise. Hyperoxia resulted in a 45% reduction in the amplitude of the V(O2) slow component during severe exercise (N: 0.60+/-0.21 L min(-1) versus H: 0.33+/-0.17 L min(-1); P < 0.05) and a 15% extension of time to exhaustion during supra-maximal exercise (N: 173+/-28 s versus H: 198+/-41 s; P < 0.05). These results indicate that the Phase II V(O2) kinetics are not normally constrained by (diffusional) O2 transport limitations during moderate, severe or supra-maximal intensity exercise in young healthy subjects performing upright cycle exercise.
- Published
- 2006
- Full Text
- View/download PDF
50. Influence of initial metabolic rate on pulmonary O2 uptake on-kinetics during severe intensity exercise.
- Author
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Wilkerson DP and Jones AM
- Subjects
- Adult, Biomechanical Phenomena, Electromyography methods, Exercise Test methods, Heart Rate physiology, Humans, Male, Pulmonary Gas Exchange physiology, Exercise physiology, Oxygen metabolism, Oxygen Consumption physiology, Physical Endurance physiology
- Abstract
We hypothesised that the fundamental (Phase II) component of pulmonary oxygen uptake (VO(2)) kinetics would be significantly slower when step transitions to severe intensity cycle exercise were initiated from elevated baseline metabolic rates, and that this would be associated with evidence for a greater activation of higher-order (i.e. type II) muscle fibres. Seven male subjects (age 22-34 years) completed repeat step transitions to a severe (S) work rate, estimated to require 100% VO(2) peak, from a baseline of: (1) 3 min of unloaded cycling (L-->S); (2) 6 min of moderate exercise (M-->S); (3) 6 min of heavy exercise (H-->S). Pulmonary gas exchange and the electromyogram (EMG) of the m. vastus lateralis were measured throughout all exercise tests. The Phase II VO(2) kinetics became progressively slower at higher baseline metabolic rates (tau was 37 +/- 6, 59 +/- 23, and 93 +/- 50 s for L-->S, M-->S, and H-->S, respectively; P < 0.05 between L-->S and H-->S). Both the integrated EMG and the mean power frequency were significantly higher immediately before the step transition to severe exercise when it was initiated from higher metabolic rates. Although indirect, these data suggest that the slower Phase II VO(2) kinetics observed at higher baseline metabolic rates was related to alterations in muscle activation and fibre recruitment patterns.
- Published
- 2006
- Full Text
- View/download PDF
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