Effect of eccentric exercise-induced muscle damage on the dynamics of muscle oxygenation and pulmonary oxygen uptake

Unaccustomed eccentric exercise has a profound impact on muscle structure and function. However, it is not known whether associated microvascular dysfunction disrupts the matching of O2delivery (Q̇o2) to O2utilization (V̇o2). Near-infrared spectroscopy (NIRS) was used to test the hypothesis that eccentric exercise-induced muscle damage would elevate the muscle Q̇o2:V̇o2ratio during severe-intensity exercise while preserving the speed of the V̇o2kinetics at exercise onset. Nine physically active men completed “step” tests to severe-intensity exercise from an unloaded baseline on a cycle ergometer before (Pre) and 48 h after (Post) eccentric exercise (100 squats with a load corresponding to 70% of body mass). NIRS and breath-by-breath pulmonary V̇o2were measured continuously during the exercise tests and subsequently modeled using standard nonlinear regression techniques. There were no changes in phase II pulmonary V̇o2kinetics following the onset of exercise (time constant: Pre, 25 ± 4 s; Post, 24 ± 2 s; amplitude: Pre, 2.36 ± 0.23 l/min; Post, 2.37 ± 0.23 l/min; all P > 0.05). However, the primary (Pre, 14 ± 3 s; Post, 19 ± 3 s) and overall (Pre, 16 ± 4 s; Post, 21 ± 4 s) mean response time of the [HHb] response was significantly slower following eccentric exercise ( P < 0.05). The slower [HHb] kinetics observed following eccentric exercise is consistent with an increased Q̇o2:V̇o2ratio during transitions to severe-intensity exercise. We propose that unchanged primary phase V̇o2kinetics are associated with an elevated Q̇o2:V̇o2ratio that preserves blood-myocyte O2flux.