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BACKGROUND The relationships between sarcopenia and postoperative outcomes in patients with early-stage gastric cancer who undergo radical gastrectomy is unclear. We aimed to investigate the predictive value of sarcopenia on adverse outcomes for stage I gastric cancer. METHODS The clinical data of patients who underwent radical gastrectomy for stage I gastric cancer between July 2013 and May 2019 were prospectively collected. Basic sarcopenia components were measured preoperatively. Univariate and multivariate analyses were conducted to evaluate the risk factors for short- and long-term outcomes. RESULTS A total of 507 patients with early-stage gastric cancer were included in the study, and 73 (14.4%) patients were diagnosed as sarcopenia. Patients with sarcopenia had significantly higher incidence of postoperative complications (32.9% vs. 17.5%, P = 0.002), longer postoperative hospital stays (13 days vs. 12 days, P

No information to date is available on the structure of fish major histocompatibility complex (MHC) class I and β2-microglobulin (β2m) proteins. In the present study, grass carp ( Ctenopharyngodon idellus ) MHC class I ( Ctid -MHC I) and β 2 -microglobulin ( Ctid -β2m) genes were expressed as soluble maltose binding protein (MBP)-proteins and purified in a pMAL-p2X/ Escherichia coli TB1 system. The expressed proteins were purified on amylase affinity columns followed by DEAE–Sepharose. The purified products were identified by Western blotting with anti-MBP polyclonal antibodies. The MBP- Ctid -MHC I and MBP- Ctid -β2m were cleaved separately with Factor Xa, mixed together and purified on DEAE–Sepharose. The secondary structures were analyzed by circular dichroism (CD) spectrophotometry. The three-dimensional (3D) structure of their peptide-binding domain (PBD) was modeled based sequence homology. The sequence lengths of the α-helix, β-sheet, turn, and random coil in the Ctid -MHC I protein were 79 aa, 75 aa, 20 aa, and 99 aa, respectively. In the 97 aa of Ctid -β2m, the contents of the α-helix, β-sheet, turn, and random coil were 0 aa, 41 aa, 12 aa, and 44 aa, respectively. The Ctid -β2m protein displayed a typical β-sheet. Homology modeling of the Ctid -MHC I and Ctid -β2m proteins demonstrated similarities with the structure of human MHC class I proteins.

The authors designed to separate, purify and determine the monosaccharide composition of the polysaccharide from Cordyceps militaris, and study its effect on reverse cholesterol transport in vivo by isotope tracing assay. Polysaccharides were separate and purify by ion exchange column Q-sepharose Fast Flow and size exclusion column Sephacryl S200HR; the molecular weight and monosaccharide composition of the polysaccharides were determined by high performance gel permeation chromatography and high performance liquid chromatography coming with pre-column derivation, respectively. Finally, three purified polysaccharides CMBW1, CMBW2 and CMYW1 were obtained, their total carbohydrate contents were 87%, 89%, 95%, respectively; their protein contents were 6.5%, 1.3%, 2.8%, respectively; their molecular weights were 772.1, 20.9, 13.2 kDa, respectively; CMBW1 was composed of mannose, glucosamine, rhamnose, glucuronic acid, glucose, galactose and arabinose with a molar ratio of 7.25: 0.17: 1.29: 0.23: 6.30: 11.08: 0.79; CMBW2 was composed of mannose, glucosamine, galactose and arabinose with a molar ratio of 2.40: 0.16: 2.92: 0.24; CMYW1 was composed of mannose, glucosamine, glucuronic acid and glucose with a molar ratio of 0.59: 0.57: 0.45: 25.61. Polysaccharide at 50 mg x kg(-1) could significantly improve the transport of 3H- cholesterol to blood and excretion from feces. All of the three purified polysaccharides CMBW1, CMBW2 and CMYW1 were heteropolysaccharide; and they could improve reverse cholesterol transport in vivo, the underlying mechanisms are being studied.

Theil index has been widely used in inequality measure for its systematic and homogeneous. This paper establishes the knowledge stock index system to calculate the knowledge stock, considering the specific economic background with fewer indexes reflecting the stock accurately and scientifically, then evaluates the knowledge stock gap based on the Theil index. The empirical analysis of 31 Chinese provinces in 2011 shows that there are differences among different regions, the evaluation of knowledge stock gap of Beijing, Jiangsu and Guangdong are significantly higher than the whole country's average level. Besides, the reasons are analyzed in detail.

The acrylamide derived from Oppolzer's camphor sultam was polymerized and the resulting polymer was converted to poly(allyl acetate) through a reduction-acetylation sequence. The poly(allyl acetate) thus obtained was analyzed by NMR and was compared with authentically prepared isotactic as well as atactic poly(tallyl acetate). The 13 C NMR peaks were assigned at the tetrad level. It is concluded that the poly(allyl acetate) obtained from poly(camphor sultam acrylamide) has an isotacticity level as low as 54%. Telomerization studies indicated that this chiral auxiliary is unable to control stereochemistry for telomers with two or more monomers incorporated. A model is proposed to account for the low selectivity in these polymerizations

Single-crystal X-ray analysis of four alkenes that undergo stereoselective radical addition reactions are reported. The facial selectivity of radical additions to these alkenes is understood based upon their solid-state conformations. Alkenes which have a conformation placing a group at a position in space sterically protecting one the faces from addition undergo radical addition with diastereofacial selectivity. The structures of the alkenes are analyzed by the polar coordinates of groups relative to the alkenes center undergoing addition

Single-crystal X-ray analysis of four alkenes that undergo stereoselective radical addition reactions are reported. The facial selectivity of radical additions to these alkenes is understood based upon their solid-state conformations. Alkenes which have a conformation placing a group at a position in space sterically protecting one the faces from addition undergo radical addition with diastereofacial selectivity. The structures of the alkenes are analyzed by the polar coordinates of groups relative to the alkenes center undergoing addition

Competition rate constants were determined for the addition of cyclohexyl radical to α,β unsaturated amides derived from pyrrolidine and trans-2,5-dimethylpyrrolidine. The relative rates can be understood by single crystal X-Ray analyses of the alkenes and an examination of the presumed transition state

The unsymmetrical azoalkane 2-(tert-butylazo)-2-(dimethylamino)propane (7) has been synthesized cleanly by nucleophilic displacement of chloride from the corresponding α-chloroazoalkane 5 with dimethylamine. Thermolysis of 7 in hydrocarbon solvents affords typical radical-derived products and exhibits activation parameters ΔH : =26.6±0.4 kcal/mol and ΔS : =−6.6±1.1 eu. Since the thermolysis rate of 7 is 10 4 faster than that of 1,2-di-tert-butyldiazene, the large stabilization of α-amino radicals is supported. Unusual products were obtained in acetonitrile, suggesting reduction of the azo linkage by 2-(dimethylamino)-2-propyl radicals and cleavage of the resulting hydrazyl radicals. In protic solvents, 7 undergoes acid catalyzed decomposition via tert-butyldiazene as a postulated intermediate

In order to develop recombinant porcine Interferon-gamma (rPoIFN-gamma) to prevent porcine viral infection, PoIFN-gamma cDNA lacking the signal peptide was expressed in Pichia pastoris GS115 strain, and the effect of rPoIFN-gamma on porcine reproductive and respiratory syndrome virus (PRRSV) was investigated. The PoIFN-gamma gene was inserted into integrative vector pHIL-S1, and the recombinant GS115 strain (pHIL-S1/PoIFN-gamma) was constructed by homologues recombinant. The rPoIFN-gamma protein was 18 kD with an expressing yield of 18% was assayed by SDS-PAGE and Western blot, respectively. The anti-viral activity of rPoIFN-gamma was in the range of 450-540 u/mL. In addition, the effect of rPoIFN-gamma ant-PRRSV was determined using CPE50 method. The results indicated that high concentration of rPoIFN-gamma could inhibit PRRSV on Marc-145 cell line. The rPoIFN-gamma is a potential drug for prevention and treatment of various kinds of viral pig diseases.

The solution-phase photolysis of ((trifluoromethyl)azo) cyclopropane (TFMACP) leads to a very different product distribution than the one reported previously. While CF 3 H was claimed to be the major product, we instead find much cis-TFMACP, 1-(trifluoromethyl)-2-pyrazoline, and (trifluoromethyl) cyclopropane. The four primary photoreactions responsible for these products are exactly analogous to those taking place in azocyclopropane

Transient CARS spectroscopy has been used to study the Raman-active ν 1 (symmetric C-D stretch) vibration-rotation band of CD 3 radicals formed by gas phase laser photolysis of azomethane- d 6 . The following spectroscopic constants have been deduced: band origin=2157.5 cm −1 , Δ B =−0.026 cm −1 , Δ C =−0.018 cm −1 , and X 12 =−5 cm −1 .