91 results on '"Wei-hua Jiang"'
Search Results
2. Cloning and Expression of Yak Active Chymosin in
- Author
-
Fan Luo, Wei Hua Jiang, Yuan Xiao Yang, Jiang Li, and Ming Feng Jiang
- Subjects
Active Chymosin ,Yak ,Expression ,Animal culture ,SF1-1100 ,Animal biochemistry ,QP501-801 - Abstract
Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production. Yaks total RNA was extracted from the abomasum of an unweaned yak. The yak preprochymosin, prochymosin, and chymosin genes from total RNA were isolated using gene specific primers based on cattle chymosin gene sequence respectively and analyzed their expression pattern byreal time-polymerase chain reaction. The result showed that the chymosin gene expression level of the sucking yaks was 11.45 times higher than one of adult yaks and yak chymosin belongs to Bovidae family in phylogenetic analysis. To express each, the preprochymosin, prochymosin, and chymosin genes were ligated into the expression vector pPICZαA, respectively, and were expressed in Pichia pastoris X33. The results showed that all the recombinant clones of P. pastoris containing the preprochymosin, prochymosin or chymosin genes could produce the active form of recombinant chymosin into the culture supernatant. Heterologous expressed prochymosin (14.55 Soxhlet unit/mL) had the highest enzyme activity of the three expressed chymosin enzymes. Therefore, we suggest that the yak chymosin gene recombinant Pichia pastoris strain could provide an alternative source of rennet production. more...
- Published
- 2016
- Full Text
- View/download PDF
Catalog
3. The actions of neonicotinoid insecticides on nicotinic acetylcholine subunits Ld α 1 and Ld α 8 of Leptinotarsa decemlineata and assembled receptors
- Author
-
Zhi‐Min Wang, Sha Li, Cheng‐Cheng Shi, Lin‐Jie Xie, Kai‐Yun Fu, and Wei‐Hua Jiang
- Subjects
Insecticides ,Nicotine ,Receptors, Nicotinic ,Nitro Compounds ,Acetylcholine ,General Biochemistry, Genetics and Molecular Biology ,RNA, Complementary ,Rats ,Coleoptera ,Neonicotinoids ,Insect Science ,Animals ,Agronomy and Crop Science ,Thiamethoxam ,Ecology, Evolution, Behavior and Systematics - Abstract
The insect nicotinic acetylcholine receptor (nAChR) is a pentameric channel protein and also a target for neonicotinoids. There are few reported studies on the molecular interactions of Leptinotarsa decemlineata nAChRs with neonicotinoids. In this study, we analyzed the response of acetylcholine and neonicotinoids (thiamethoxam [TMX], imidacloprid [IMI], and clothianidin [CLO]) on hybrid receptors constructed by nAChR α1 and α8 subunits of L. decemlineata (Ldα1 and Ldα8) co-expressed with rat β2 subunit (rβ2) at different capped RNA (cRNA) ratios in Xenopus oocytes. In addition, we evaluated the expression changes of Ldα1 and Ldα8 after median lethal dose of TMX treatment for 72 h by quantitative polymerase chain reaction (qPCR). The resulting functional nAChRs Ldα1/rβ2 and Ldα1/Ldα8/rβ2 showed different pharmacological characteristics. The neonicotinoids tested showed lower agonist affinity on Ldα1/Ldα8/rβ2 compared to Ldα1/rβ2 at same ratios of subunit cRNAs. The sensitivities of neonicotinoids tested for Ldα1/rβ2 and Ldα1/Ldα8/rβ2 at cRNA ratios of 5:1, 1:1 and 5:5:1, 1:1:1, respectively, were lower than those for nAChRs at ratios of 1:5 and 1:1:5, respectively, whereas the values of maximum response (I more...
- Published
- 2022
- Full Text
- View/download PDF
4. Research on System Integrative Evaluation Method Based on Survivability.
- Author
-
Wei-Hua Jiang, Jun Du, and Feng Liu
- Published
- 2008
- Full Text
- View/download PDF
5. [Risk Factor Analysis of Abdominal Aortic Enlargement after Thoracic Endovascular Aortic Repair Using Two-Stent Graft Implantation for Stanford Type B Aortic Dissection]
- Author
-
Yong-Shan, Gao, Wei-Hua, Jiang, Yan-Jun, Yang, Zhen-Ming, Zhang, Feng-Xian, Jin, Yue-Hua, Dong, Da-Wei, Wang, and Yu-Lei, Wei
- Subjects
Aortic Dissection ,Blood Vessel Prosthesis Implantation ,Treatment Outcome ,Aortic Aneurysm, Thoracic ,Risk Factors ,Endovascular Procedures ,Humans ,Stents ,Thrombosis ,Factor Analysis, Statistical ,Aortography ,Blood Vessel Prosthesis ,Retrospective Studies - Abstract
To explore the risk factors of abdominal aortic enlargement (AAE) after thoracic endovascular aortic repair using two-stent graft implantation (TEVAR-TSI) for Stanford type B aortic dissection.The clinical and imaging data of patients who underwent TEVAR-TSI for Stanford type B aortic dissection in the First Affiliated Hospital of Hebei North University from January 2013 through September 2020 were retrospectively collected and analyzed. CT angiography (CTA) scans were performed before the procedure. Follow-up CTA scans were scheduled and performed in 1, 3, 6, and 12 months after the procedure and annually thereafter. The primary outcome was AAE. The risk factors of AAE after TEVAR-TSI were selected and survival analysis and multivariate logistic regression were conducted accordingly.A total of 146 patients were regularly followed up at our hospital, with the median followup time of the entire cohort being 48 months (ranging from 12 to 84 months). During the followup period after TEVAR-TSI, the incidence of AAE was 19.9% (29/146). A total of 29 patients developed AAE (the AAE group), while 117 patients did not develop AAE (the non-AAE group). There were a total of 27 deaths, including 13 in the non-AAE group versus 14 in the AAE group. Distal aortic reoperation was performed on 10 patients, including 4 in the non-AAE group versus 6 in the AAE group. The cumulative long-term survival and freedom from distal aortic reoperation of the non-AAE group were both significantly better those of the AAE group (The prognosis of patients who developed AAE after TEVAR-TSI was not good. During followup visits, as precautions against the development of AAE, close attention should be paid to partial thrombosis of the false lumen, cumulative diameter of residual intimal tear above the level of the lowest renal arteries, and cumulative diameter of residual intimal tear below the level of the lowest renal arteries. more...
- Published
- 2022
6. Resistance to neonicotinoid insecticides and expression changes of eighteen cytochrome P450 genes in field populations of <scp> Bemisia tabaci </scp> from Xinjiang, China
- Author
-
Mei‐Na Wang, Tursun Ahmat, Qiang Wang, Wei‐Hua Jiang, Lin‐Jie Xie, and Zun‐Zun Jia
- Subjects
0106 biological sciences ,0303 health sciences ,Veterinary medicine ,biology ,media_common.quotation_subject ,Neonicotinoid ,Cytochrome P450 ,Insect ,Monooxygenase ,01 natural sciences ,010602 entomology ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry ,Imidacloprid ,Insect Science ,biology.protein ,Bioassay ,Thiamethoxam ,Gene ,030304 developmental biology ,media_common - Abstract
The occurrence of Bemisia tabaci poses an increasingly serious threat to cotton and vegetable crops in Xinjiang, China. Currently, neonicotinoid insecticides are commonly used to control the insect, to which resistance is inevitable due to intensive use. However, the resistance status and mechanism of B. tabaci to neonicotinoid insecticides in Xinjiang are poorly understood. Cytochrome P450 monooxygenases represent a key detoxification mechanism in the neonicotinoid resistance of B. tabaci. In this study, the resistance level to imidacloprid and thiamethoxam was investigated using the leaf dipping method in five field populations of B. tabaci from Turpan (TP, two sampling sites), Shache (SC), Hotan (HT) and Yining (YN) in northern and southern Xinjiang. The expression changes of eighteen cytochrome P450 genes from the select B. tabaci populations were determined by real‐time fluorescence quantitative PCR (qPCR). The bioassay revealed that the five populations tested had developed moderate to high levels of resistance to imidacloprid (12.26–46.07‐fold), while the populations remained sensitive to thiamethoxam except for HT, which had a low level of resistance. The qPCR results showed that the expression levels of five P450 genes, CYP4G68, CYP6CM1, CYP303A1‐like, CYP6DZ7 and CYP6DZ4, were significantly higher in some resistant field populations than in the susceptible strain. Resistance to imidacloprid in field populations of B. tabaci might be associated with the increased expression of these five cytochrome P450 genes. The results are useful for further understanding the mechanism of neonicotinoid resistance and will contribute to the management of insecticide‐resistant B. tabaci in Xinjiang. more...
- Published
- 2020
- Full Text
- View/download PDF
7. CAP Analysis of the Distribution of the Introduced Bemisia tabaci (Hemiptera: Aleyrodidae) Species Complex in Xinjiang, China and the Southerly Expansion of the Mediterranean Species
- Author
-
Zun‐Zun Jia, Tursun Ahmat, Wei‐Hua Jiang, Kai-Yun Fu, Ding Xinhua, Wang Xiaowu, Wen-Chao Guo, and Jiang He
- Subjects
Gene Flow ,AcademicSubjects/SCI01382 ,0106 biological sciences ,China ,Species complex ,Zoology ,Genes, Insect ,Biology ,Polymerase Chain Reaction ,Bemisia tabaci ,01 natural sciences ,invasive species ,Gene flow ,Hemiptera ,03 medical and health sciences ,Cleaved amplified polymorphic sequence ,Genetic variation ,Animals ,Research Articles ,030304 developmental biology ,cryptic species ,0303 health sciences ,Genetic diversity ,Asia, Eastern ,Genetic Variation ,genetic diversity ,General Medicine ,Interspecific competition ,biology.organism_classification ,010602 entomology ,Insect Science ,Biological dispersal ,Introduced Species ,Animal Distribution - Abstract
Bemisia tabaci (Gennadius) cryptic complex has invaded Xinjiang, China, since 1998. The distribution of Mediterranean (MED) and Middle East-Asia Minor 1 (MEAM1) B. tabaci substrains has been gradually identified due to the development of molecular technology. In this study, the distribution of MED and MEAM1 in Xinjiang was determined by cleaved amplified polymorphic sequence (CAPs). Results showed that MED dominated in northern Xinjiang (84%), whereas MEAM1 was dominant in southern Xinjiang (72%). Five pairs of simple sequence repeat (SSR) primers were used to analyze the genetic diversity of B. tabaci among 36 geographic populations. The genetic diversity of MED and MEAM1was low and varied little among populations in Xinjiang (0.09 ± 0.14 and 0.09 ± 0.13, respectively). Based on ∆K statistic, 13 populations of MEAM1 could be classified into two subgroups at K = 2, whereas the 23 populations of MED could be classified into four subgroups at K = 4. However, Mantel t-test demonstrated no correlation between geographical and genetic distances among B. tabaci complex (R = 0.42, P = 1.00). Neighbor-joining and principal coordinate analysis showed that geographical isolation and interspecific differences were the main causes of the genetic variation. Gene flow predicted that MEAM1 was most likely introduced from Urumqi to the southern Xinjiang. Meanwhile, a large proportion of MED in Kashi region came from Changji and Yining. To block ongoing dispersal, strict detection and flower quarantine regulations need to be enforced. more...
- Published
- 2021
- Full Text
- View/download PDF
8. [The Characteristics of Aortic Remodeling after Thoracic Endovascular Aortic Repair using Two-stent Graft Implantation for Stanford Type B Aortic Dissection]
- Author
-
Yong-Shan, Gao, Zhen-Ming, Zhang, Feng-Xian, Jin, Yue-Hua, Dong, Wei-Hua, Jiang, Da-Wei, Wang, and Yu-Lei, Wei
- Subjects
Aortic Dissection ,Blood Vessel Prosthesis Implantation ,Treatment Outcome ,Aortic Aneurysm, Thoracic ,Endovascular Procedures ,Humans ,Aorta, Thoracic ,Stents ,Retrospective Studies - Abstract
To investigate the characteristics of aortic remodeling after thoracic endovascular aortic repair using two-stent graft implantation (TEVAR-TSI) for Stanford B aortic dissection.The clinical and imaging data of 128 patients who underwent TEVAR-TSI for Stanford B aortic dissection in the First Affiliated Hospital of Hebei North University from January 2013 through May 2019 were retrospectively collected. CT images were obtained before (TThe stented segment of the descending thoracic aorta was evaluated (LAfter receiving TEVAR-TSI, Stanford type B aortic dissection patients had high thrombosis absorption rate in the thoracic aortic segment covered by stent, and the aortic remodeling was more ideal. The aortic remodeling effect in the abdominal aortic segment not covered was not ideal, and the inner diameter of the abdominal aorta tended to increase. Therefore, close follow-up monitoring should be conducted. more...
- Published
- 2021
9. KRT8 upregulation promotes tumor metastasis and is predictive of a poor prognosis in clear cell renal cell carcinoma
- Author
-
Deng-Shuang Wu, Li Gao, Lijun Ma, Shi Jiazi, Yi He, Bing Liu, Wei-hua Jiang, Hai-song Tan, Zhenjie Wu, De-Sheng Wang, Yi Bao, and Lin-hui Wang
- Subjects
0301 basic medicine ,Oncology ,medicine.medical_specialty ,Pathology ,clear cell renal cell carcinoma ,Metastasis ,03 medical and health sciences ,Internal medicine ,medicine ,metastasis ,Autocrine signalling ,KRT8 ,Tissue microarray ,business.industry ,Cell migration ,medicine.disease ,Clear cell renal cell carcinoma ,030104 developmental biology ,IL-11 ,Keratin 8 ,biomarker ,Immunohistochemistry ,Biomarker (medicine) ,business ,Research Paper - Abstract
// Hai-Song Tan 1, * , Wei-Hua Jiang 2, * , Yi He 3, * , De-Sheng Wang 4, * , Zhen-Jie Wu 1 , Deng-Shuang Wu 1 , Li Gao 5 , Yi Bao 1 , Jia-Zi Shi 1 , Bing Liu 1 , Li-Jun Ma 2 and Lin-Hui Wang 1 1 Department of Urology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China 2 Department of Oncology, Shanghai Tongren Hospital, Shanghai Jiaotong University, Shanghai 200336, China 3 Department of Urology, Jiaxing First Hospital, Zhejiang 314000, China 4 Department of Urology, Second People's Hospital of Bengbu City, Anhui 233000, China 5 Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China * These authors have contributed equally to this work Correspondence to: Lin-Hui Wang, email: wanglinhuicz@163.com Li-Jun Ma, email: lijun.ma@shtrhospital.com Bing Liu, email: 13501616398@163.com Keywords: clear cell renal cell carcinoma, metastasis, KRT8, IL-11, biomarker Received: January 17, 2017 Accepted: June 19, 2017 Published: July 12, 2017 ABSTRACT Keratin 8 (KRT8) plays an essential role in the development and metastasis of multiple human cancers. However, its role in clear cell renal cell carcinoma (ccRCC) remains unexplored. Here, we investigated the expression pattern, clinical significance, and function of KRT8 in ccRCC. KRT8 mRNA and protein levels were determined in two large cohorts using quantitative real-time polymerase chain reaction (qRT-PCR) and tissue microarray (TMA) immunohistochemistry (IHC), respectively. We found that KRT8 expression was upregulated in ccRCC and vein tumor thrombi (VTTs). KRT8 overexpression in ccRCC was significantly correlated with aggressive characteristics and was predictive of a poor prognosis in ccRCC patients. Moreover, KRT8 overexpression in renal cancer cell lines promoted cell migration and invasion. In contrast, KRT8 knockdown suppressed ccRCC metastasis both in vitro and in vivo . In addition, our findings showed that KRT8 promoted ccRCC metastasis by increasing IL-11 expression, causing IL-11 autocrine induction, and triggering STAT3 signaling. Overall, this study established the significance of KRT8-IL-11 axis activation in aggressive ccRCC and defined a novel critical signaling mechanism that drives human ccRCC invasion and metastasis. more...
- Published
- 2017
- Full Text
- View/download PDF
10. Comparative study of catalytic properties in three Tibetan ruminant stomach lysozymes.
- Author
-
Guo Xia, Yi Li Liu, Wei Hua Jiang, Jung Nam Lee, and Ming Feng Jiang
- Subjects
POLYACRYLAMIDE gel electrophoresis ,LYSOZYMES ,STOMACH ,TIBETANS ,RUMINANTS ,IONIC strength ,GEL electrophoresis - Abstract
The stomach lysozymes of ruminants play a paramount role in digestion and offer the chance to probe evolutionary changes in complex organisms on a biochemical basis. In this paper, we focus on the characterization of the catalytic properties of Tibetan ruminant (TR) stomach lysozymes and provide a comparative study of TR stomach lysozymes and nonplateau ruminant stomach lysozymes. The stomach lysozymes were purified with a Carboxymethyl (CM) Sepharose Fast Flow (FF) column and a Bio-Gel P-100 column. The purified stomach lysozymes were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight (MALDITOF) analyses. The antimicrobial activity was evaluated using an agar diffusion method, and the pH, ion strength, temperature, pepsin and trypsin effects on enzymatic activities were evaluated by normal biochemistry methods. The stomach lysozymes of TRs (cattle:TC, yak:TY, and sheep:TS) exhibit maximum activity at pH 5 and ionic strengths below 0.02. TC and TS stomach lysozymes are more resistant to higher temperatures than nonplateau lysozymes. Stomach lysozymes of TRs exhibit greater enzymatic activity than nonplateau lysozymes, in the presence of pepsin and trypsin. Stomach lysozymes from TS retain 86% of their initial enzymatic activity against pepsin inactivation. After trypsin treatment, the stomach lysozymal activities of TRs were significantly affected by trypsin inactivation, with the exception of TS, whose enzymatic activities in the presence of trypsin were 5- and 10-fold higher than those of nonplateau lysozymes. The stomach lysozymes of TRs are more resistant to environmental factors such as pH, temperature, pepsin, and trypsin than nonplateau lysozyme C. [ABSTRACT FROM AUTHOR] more...
- Published
- 2022
- Full Text
- View/download PDF
11. Preparation of COD Sorbent for Wastewater by Fly Ash and Straw
- Author
-
Hao-nan Wang, Hai Zhao, Nan Wang Nan Wang, Kai-rui Dong Kai-rui Dong, Mei-yang Li, Hua-jiang Wei Hua-jiang Wei, Jing Li, and Hao Wang
- Subjects
animal structures ,Sorbent ,Carbonization ,fungi ,Adsorption effect ,food and beverages ,chemistry.chemical_element ,Zinc ,Straw ,Pulp and paper industry ,Agricultural waste ,Wastewater ,chemistry ,Fly ash - Abstract
The sorbent was prepared by agricultural waste straw and power plant waste fly ash. The sorbent was used to remove COD from wastewater. The straw is crushed and carbonized. Fly ash is modified by zinc chloride activation. Result showed that the adsorption effect of the sorbent exceeds 90%. more...
- Published
- 2019
- Full Text
- View/download PDF
12. Construction of hybrid DNAs@CP for the rapid synchronous sensing of multiplex microRNAs based on experimental studies and molecular simulation
- Author
-
Wei-Hua Jiang, Yue Fan, Jin-Xiang Chen, Zi-Chuan Yang, Rong-Tian Li, Ting-Wei Chen, Min-Min Li, and Tao-Rui Wang
- Subjects
010405 organic chemistry ,Chemistry ,Coordination polymer ,Observable ,Molecular simulation ,DNA ,Molecular Dynamics Simulation ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Force field (chemistry) ,0104 chemical sciences ,Inorganic Chemistry ,MicroRNAs ,chemistry.chemical_compound ,Coordination Complexes ,Chemical physics ,Nucleic acid ,Multiplex ,Interaction mode ,Electronic energy - Abstract
A water stable one-dimensional (1D) ladder-shaped coordination polymer (CP) has been synthesized and exhibits a strong affinity to two fluorescein-tagged single-stranded probe DNAs (P-DNAs), giving a sensing platform of P-DNAs@1. Such a hybrid sensing platform is capable of simultaneous detection of breast cancer related microRNA-221 (miRNA-221) and miRNA-222 in a specific and synchronous manner, without observable cross-reactions, as supported by experimental evidences. The interaction mode and the electronic energy between CP 1 with nucleic acid were confirmed by molecular simulation and the universal force field (UFF). more...
- Published
- 2020
- Full Text
- View/download PDF
13. Preparation of COD Sorbent for Wastewater by Fly Ash and Straw
- Author
-
Kai-rui DONG, Kai-rui DONG, primary, Nan WANG, Nan WANG, additional, Hua-jiang WEI, Hua-jiang WEI, additional, WANG, Hao-nan, additional, LI, Mei-yang, additional, LI, Jing, additional, WANG, Hao, additional, and ZHAO, Hai, additional more...
- Published
- 2019
- Full Text
- View/download PDF
14. Germline mutations in 40 cancer susceptibility genes among Chinese patients with high hereditary risk breast cancer
- Author
-
Jun-Yan, Li, Ruilin, Jing, Hongyi, Wei, Minghao, Wang, Qi, Xiaowei, Haoxi, Liu, Liu, Jian, Jiang-Hua, Ou, Wei-Hua, Jiang, Fu-Guo, Tian, Yuan, Sheng, Heng-Yu, Li, Hong, Xu, Rui-Shan, Zhang, Ai-Hua, Guan, Ke, Liu, Hong-Chuan, Jiang, Yu, Ren, Jian-Jun, He, Weiwei, Huang, Ning, Liao, Xiangjun, Cai, Jia, Ming, Rui, Ling, Yan, Xu, Chun-Yan, Hu, Jianguo, Zhang, Baoliang, Guo, Lizhi, Ouyang, Ping, Shuai, Zhenzhen, Liu, Ling, Zhong, Zhen, Zeng, Ting, Zhang, Zhaoling, Xuan, Xuanni, Tan, Junbin, Liang, Qinwen, Pan, Li, Chen, Fan, Zhang, Lin-Jun, Fan, Yi, Zhang, Xinhua, Yang, Jing, BoLi, Chongjian, Chen, and Jun, Jiang more...
- Subjects
Adult ,Asian People ,Humans ,Breast Neoplasms ,Female ,Genetic Predisposition to Disease ,Middle Aged ,Germ-Line Mutation - Abstract
Multigene panel testing of breast cancer predisposition genes have been extensively conducted in Europe and America, which is relatively rare in Asia however. In this study, we assessed the frequency of germline mutations in 40 cancer predisposition genes, including BRCA1 and BRCA2, among a large cohort of Chinese patients with high hereditary risk of BC. From 2015 to 2016, consecutive BC patients from 26 centers of China with high hereditary risk were recruited (n = 937). Clinical information was collected and next-generation sequencing (NGS) was performed using blood samples of participants to identify germline mutations. In total, we acquired 223 patients with putative germline mutations, including 159 in BRCA1/2, 61 in 15 other BC susceptibility genes and 3 in both BRCA1/2 and non-BRCA1/2 gene. Major mutant non-BRCA1/2 genes were TP53 (n = 18), PALB2 (n = 11), CHEK2 (n = 6), ATM (n = 6) and BARD1 (n = 5). No factors predicted pathologic mutations in non-BRCA1/2 genes when treated as a whole. TP53 mutations were associated with HER-2 positive BC and younger age at diagnosis; and CHEK2 and PALB2 mutations were enriched in patients with luminal BC. Among high hereditary risk Chinese BC patients, 23.8% contained germline mutations, including 6.8% in non-BRCA1/2 genes. TP53 and PALB2 had a relatively high mutation rate (1.9 and 1.2%). Although no factors predicted for detrimental mutations in non-BRCA1/2 genes, some clinical features were associated with mutations of several particular genes. more...
- Published
- 2017
15. Production of Wilson Disease Model Rabbits with Homology-Directed Precision Point Mutations in the ATP7B Gene Using the CRISPR/Cas9 System
- Author
-
Xingxu Huang, Lili Liu, Jing Zhou, Qiurong Chang, Zhengwen Ma, Li Fu, Huiyang Wang, Fengying Xing, Shangang Li, Xuejin Chen, Zhenfu Fang, Yao Li, and Wei-hua Jiang
- Subjects
0301 basic medicine ,Microinjections ,Zygote ,Genetic enhancement ,Mutant ,lcsh:Medicine ,DNA, Single-Stranded ,Biology ,Homology (biology) ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Hepatolenticular Degeneration ,CRISPR ,Animals ,Humans ,Point Mutation ,Allele ,lcsh:Science ,Multidisciplinary ,Cas9 ,Point mutation ,lcsh:R ,Molecular biology ,Disease Models, Animal ,030104 developmental biology ,chemistry ,Liver ,Oligodeoxyribonucleotides ,Copper-Transporting ATPases ,Mutagenesis, Site-Directed ,lcsh:Q ,Rabbits ,CRISPR-Cas Systems ,DNA ,Copper - Abstract
CRISPR/Cas9 has recently been developed as an efficient genome engineering tool. The rabbit is a suitable animal model for studies of metabolic diseases. In this study, we generated ATP7B site-directed point mutation rabbits to simulate a major mutation type in Asians (p. Arg778Leu) with Wilson disease (WD) by using the CRISPR/Cas9 system combined with single-strand DNA oligonucleotides (ssODNs). The efficiency of the precision point mutation was 52.94% when zygotes were injected 14 hours after HCG treatment and was significantly higher than that of zygotes injected 19 hours after HCG treatment (14.29%). The rabbits carrying the allele with mutant ATP7B died at approximately three months of age. Additionally, the copper content in the livers of rabbits at the onset of WD increased nine-fold, a level similar to the five-fold increase observed in humans with WD. Thus, the efficiency of precision point mutations increases when RNAs are injected into zygotes at earlier stages, and the ATP7B mutant rabbits are a potential model for human WD disease with applications in pathological analysis, clinical treatment and gene therapy research. more...
- Published
- 2017
16. Servo Drive Trajectory Design of Three-Axis Laser Processing System
- Author
-
Yuan Cheng Fan, Wei Hua Jiang, and Da Zeng
- Subjects
Engineering ,business.industry ,Control engineering ,General Medicine ,Linkage (mechanical) ,Servomotor ,Linear interpolation ,law.invention ,law ,Control theory ,Integrator ,Trajectory ,Servo drive ,business ,Machine control - Abstract
Three-axis processing of laser inner engraving machine control system can carry on multi-axis linkage control. Furthermore, it ensures multi-axis of high feeding speed and trajectory control of high precision. This article introduces the design of laser inner engraving machine movement control system. The core is a digital integrator of linear interpolation algorithm to generate the desired trajectory and control XYZ axis linkage. Wide speed range of servo motor and position detector resolution is adequate to constitute a half-closed loop drive. With integral separation algorithm being introduced in the controller, the speed of numerical control system, track speed and positioning accuracy are guaranteed in order to achieve optimal specification for dynamic and static characteristics of the whole system. more...
- Published
- 2014
- Full Text
- View/download PDF
17. Double Position Loop Design of AC Servo Drive System
- Author
-
Yuan Cheng Fan, Zheng Liu, and Wei Hua Jiang
- Subjects
Engineering ,business.industry ,Control engineering ,General Medicine ,Servomotor ,Servomechanism ,Motion control ,AC motor ,law.invention ,Control theory ,law ,Servo bandwidth ,Delay-locked loop ,Servo drive ,business - Abstract
The loop controller of double position fully closed makes the AC servo drive system acquire a very high position accuracy and speed following which is hard to be achieved for time-varying parameters, load disturbance, AC motors and severely nonlinear controlled objects. For the system, the dynamic nature can be ensured by the inner position loop adopting PI regulation of half closed loop structure, while the stability and the immunity can be achieved by closed loop structures with the rings outside adopting dual-mode fuzzy control. This article analyzes the cause of positioning errors for servo motor and semi-closed loop structure. The article also explains how to design a two position closed-loop servo system based on fuzzy control by adopting the AC servo motor drive, the motor shaft back-end incremental photoelectric encoder to form the inner position loop and using the motion control card, the inner ring of servo system and the work platform of grating ruler to make up the outer position loop. Based on double position loop composed of three-dimensional laser processing gear, the accuracy of the three-axis linkage workbench position control and the speed following can be ensured, thus meeting the requirement of the laser subsurface engraving machine for high speed and high precision motion. more...
- Published
- 2014
- Full Text
- View/download PDF
18. Converse Comparison Theorems for Backward Stochastic Differential Equations
- Author
-
Xiao Jie Liu, Wei Hua Jiang, and Xiao Qin Huang
- Subjects
Stochastic partial differential equation ,Comparison theorem ,Stochastic differential equation ,Mathematics::Probability ,Converse theorem ,Mathematical analysis ,Converse ,Mathematics::Optimization and Control ,Applied mathematics ,General Medicine ,Sturm–Picone comparison theorem ,Mathematics ,Generator (mathematics) - Abstract
In this note, we establish a converse comparison theorem for backward stochastic differential equations (BSDEs).
- Published
- 2013
- Full Text
- View/download PDF
19. Design of Servo Positioning System Based on PID Variable Structure
- Author
-
Da Zeng, Zheng Liu, Wei Hua Jiang, and Yuan Cheng Fan
- Subjects
Engineering ,Positioning system ,business.industry ,Servo control ,PID controller ,Control engineering ,Robotics ,General Medicine ,Servomotor ,Control theory ,Control system ,Servo drive ,Artificial intelligence ,business ,Servo - Abstract
The direct-drive servo motor is servo product with high performance widely applied in the fields of robotics and manufacturing due to its special construction and high-precision design. Considering the working principles of laser engraving machines, as well as the impact on the dynamic and static performance of the control system exerted by PID controllers frequently integral correction link in the process of start and stop, this article describes in detail how to achieve accurate positioning of direct drive servo motor by adjusting PID parameters. Key Words Servo motor,PID variable structure,Servo positioning,Laser engraving machine more...
- Published
- 2013
- Full Text
- View/download PDF
20. Colorado Potato Beetle Leptinotarsa decemlineata (Say)
- Author
-
Jiahe Wu, Tuerxun Ahemaiti, Wei-Hua Jiang, Guo-Qing Li, Kai-Yun Fu, Wenchao Guo, and Chao Li
- Subjects
0106 biological sciences ,Integrated pest management ,Resistance (ecology) ,Ecology ,fungi ,Colorado potato beetle ,Biological pest control ,food and beverages ,Diapause ,Biology ,biology.organism_classification ,01 natural sciences ,Predation ,010602 entomology ,surgical procedures, operative ,Biological dispersal ,Leptinotarsa ,circulatory and respiratory physiology ,010606 plant biology & botany - Abstract
Colorado potato beetle (CPB) was naturally dispersed from Kazakhstan into Xinjiang Autonomous Region of China in 1993. Since then, it has been widely distributed all over the southern region of the Tianshan Mountain. Chinese scientists focused on its monitoring and invasion risk management in China, as well as its invasion biology and ecology related to rapid dispersal, such as developmental threshold and cumulative temperature, diapause condition, and influence factors for flight. In invaded regions of China, several techniques such as improved crop cultivation techniques, friendly environmental chemical control (low or none toxic insecticides), biological control, physical techniques, ecological regulations etc. can be combined into an integrated pest management of CPB. However, some questions still remain in theses fields, for example, the genetic variations, environment (hosts, habitats, climates, soil etc.) adaptabilities and geographical populations of CPB are still unclear, and mechanisms of CPB’s rapid resistance development to pesticides have not been well understood. The concerned diapause mechanisms are still unknown. Moreover, the interactions between CPB, hosts, pathogens, predators and parasitoids, environments have not been studied, and the resistance or tolerance of potato plants to CPB and their related mechanisms all need to be understood in order to breed CPB-resistant potato crops. more...
- Published
- 2017
- Full Text
- View/download PDF
21. CBX6 overexpression contributes to tumor progression and is predictive of a poor prognosis in hepatocellular carcinoma
- Author
-
Guanglei Qiao, Hai-song Tan, Hao Xing, Lin-Hui Wang, Jun Han, Yuan Yang, Sheng-yu Huang, Zhen-guang Wang, Shuai Li, Weiping Zhou, Tao Tian, Lijun Ma, Rong Gao, Hao Zheng, Wei-hua Jiang, Hao Ren, Jun-sheng Ni, and Ying Chen more...
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Adult ,Male ,Pathology ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,proliferation ,Blotting, Western ,Mice, Nude ,Polycomb-Group Proteins ,Kaplan-Meier Estimate ,Polymerase Chain Reaction ,S100A9 ,Disease-Free Survival ,03 medical and health sciences ,Mice ,In vivo ,medicine ,Biomarkers, Tumor ,Animals ,Humans ,Aged ,Mice, Inbred BALB C ,Cell growth ,business.industry ,Liver Neoplasms ,hepatocellular carcinoma ,Middle Aged ,medicine.disease ,Prognosis ,Immunohistochemistry ,digestive system diseases ,030104 developmental biology ,Oncology ,Tumor progression ,Tissue Array Analysis ,Hepatocellular carcinoma ,Cancer research ,Disease Progression ,CBX6 ,Biomarker (medicine) ,Heterografts ,biomarker ,Female ,business ,Research Paper - Abstract
Aberrant chromobox (CBX) family protein expression has been reported in a variety of human malignancies. However, the role of CBX6 in hepatocellular carcinoma (HCC) progression and patient prognosis remains unknown. In this study, we found that CBX6 was frequently up-regulated in HCC clinical samples and HCC cell lines and that CBX6 expression was significantly correlated with larger tumor sizes (≥ 5 cm, p = 0.011) and multiple tumors (n ≥ 2, p = 0.018). Survival analyses indicated that patients with higher CBX6 expression levels had significantly shorter recurrence-free survival (RFS) and overall survival (OS) than patients with lower CBX6 expression levels, and multivariate analyses confirmed that increased CBX6 expression was an independent unfavorable prognostic factor for HCC patients. Functional study demonstrated that CBX6 profoundly promoted HCC cell growth both in vitro and in vivo, and mechanistic investigation revealed that the S100A9/NF-κB/MAPK pathway was essential for mediating CBX6 function. In conclusion, our results represent the first evidence that CBX6 contributes to tumor progression and indicate that the protein may serve as a novel prognostic biomarker for HCC and as a therapeutic target in the treatment of the disease. more...
- Published
- 2016
22. TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
- Author
-
Lijun Ma, Tao Tian, Rong Gao, Cheng Chen, Hao Ren, Jun Han, Jian Wang, Hao Zheng, Wei-hua Jiang, Mengchao Wang, Weiping Zhou, Shuai Li, and Yuan Yang
- Subjects
0301 basic medicine ,Oncology ,Male ,STAT3 Transcription Factor ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Colorectal cancer ,Vesicular Transport Proteins ,Mice, Nude ,Article ,Metastasis ,03 medical and health sciences ,Downregulation and upregulation ,In vivo ,Cell Movement ,Internal medicine ,medicine ,Animals ,Humans ,Neoplasm Metastasis ,neoplasms ,Gene knockdown ,Mice, Inbred BALB C ,Multidisciplinary ,business.industry ,Liver Neoplasms ,Cell migration ,Middle Aged ,medicine.disease ,Interleukin-11 ,digestive system diseases ,Up-Regulation ,Interleukin 11 ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Hepatocellular carcinoma ,Gene Knockdown Techniques ,Cancer research ,Disease Progression ,Female ,business - Abstract
Transmembrane p24 trafficking protein 3(TMED3) is a metastatic suppressor in colon cancer, but its function in the progression of hepatocellular carcinoma (HCC) is unknown. Here, we report that TMED3 was up-regulated in HCC and portal vein tumor thrombus. TMED3 up-regulation in HCC was significantly correlated with aggressive characteristics and predicted poor prognosis in HCC patients. TMED3 overexpression in HCC cell lines promoted cell migration and invasion. In contrast, TMED3 knockdown suppressed HCC metastasis both in vitro and in vivo. Gene microarray analysis revealed decreased IL-11 expression in TMED3-knockdown cells. We propose that TMED3 promotes HCC metastasis through IL-11/STAT3 signaling. Taken together, these findings demonstrate that TMED3 promotes HCC metastasis and is a potential prognostic biomarker in HCC. more...
- Published
- 2016
- Full Text
- View/download PDF
23. miR-598 inhibits metastasis in colorectal cancer by suppressing JAG1/Notch2 pathway stimulating EMT
- Author
-
Lijun Ma, Guanglei Qiao, Peihua Ni, Haichen Zhang, Ying Chen, Wei-hua Jiang, Jia Chen, and Xiangfan Liu
- Subjects
0301 basic medicine ,JAG1 ,Epithelial-Mesenchymal Transition ,Cell ,Blotting, Western ,Notch signaling pathway ,Fluorescent Antibody Technique ,Mice, Nude ,Apoptosis ,Biology ,Bioinformatics ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Cell Movement ,microRNA ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,Epithelial–mesenchymal transition ,RNA, Messenger ,Receptor, Notch2 ,Neoplasm Metastasis ,Cell Proliferation ,Gene knockdown ,Mice, Inbred BALB C ,Reverse Transcriptase Polymerase Chain Reaction ,Cell migration ,Cell Biology ,Xenograft Model Antitumor Assays ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer research ,Signal transduction ,Colorectal Neoplasms ,Jagged-1 Protein ,Signal Transduction - Abstract
MicroRNAs (miRNAs) are a class of endogenous, evolutionarily conserved small non-coding RNA molecules that mediate the posttranscriptional process of target gene, leading to translational repression or degradation of target mRNAs. A series of studies have indicated that miRNAs play an important role in tumor initiation, development and progression. In this study, we found that down regulation of miR-598 was a frequent event in CRC tissues compared to the paracarcinoma tissues. And the study demonstrated that miR-598 was implicated in CRC metastasis. Transwell migration assay revealed that elevated miR-598 expression reduces CRC cell migration. Moreover, our study showed that suppression of miR-598 expression induces CRC cell epithelialmesenchymal transition(EMT) and overexpression of miR-598 inhibits CRC cell EMT. In addition, bioinformatics target prediction identified JAG1 as a putative target of miR-598. Knockdown of miR-598 was shown to upregulate JAG1 expression. Furthermore, overexpression of miR-598 suppressed the expression of JAG1. Consistent results were also obtained when the regulation of JAG1 expression by miR-598 was further specified in CRC tissues. Moreover, overexpression of JAG1 induces epithelialmesenchymal transition(EMT) and promotes the metastasis of CRC cells. Decreased Notch2 expression suppresses CRC cells metastasis and EMT. Together, these results indicate that miR-598 is a novel regulator of colorectal cancer metastasis. Our data suggest miR-598 is implicated in regulating Epithelial-mesenchymal transitions by directly suppressing its downstream target gene JAG1 to inactivate Notch signaling pathway. more...
- Published
- 2016
24. Singularity analysis of Jeffcott rotor-magnetic bearing with time delays
- Author
-
Xiu-yan Xu and Wei-hua Jiang
- Subjects
Bearing (mechanical) ,Singularity ,law ,Rotor (electric) ,Control theory ,Applied Mathematics ,Characteristic equation ,Magnetic bearing ,Gravitational singularity ,Bifurcation ,Center manifold ,law.invention ,Mathematics - Abstract
A Jeffcott rotor-magnetic bearing with time delays is investigated in this paper. Firstly, it is found that the characteristic equation of the system satisfies the conditions of the singularity. Secondly, the center manifold reduction and normal form are employed to study the bifurcation from simple zero and zero-purely imaginary singularities. The results of this paper will help to understand the influence of the time delays in feedback loop on the dynamics of rotor-magnetic bearing system. more...
- Published
- 2012
- Full Text
- View/download PDF
25. Efficacy of endosulfan and fipronil and joint toxic action of endosulfan mixtures against Leptinotarsa decemlineata (Say)
- Author
-
Wen-Jun Fu, Man-Hui Xiong, Xiao-Qin Shi, Guo-Qing Li, Zhi-Tian Wang, Zhen-Han Xia, Wen-Chao Guo, and Wei-Hua Jiang
- Subjects
Larva ,Pyrethroid ,biology ,Colorado potato beetle ,biology.organism_classification ,Toxicology ,chemistry.chemical_compound ,chemistry ,Phoxim ,Toxicity ,Agronomy and Crop Science ,Leptinotarsa ,Endosulfan ,Fipronil - Abstract
The Colorado potato beetle (Leptinotarsa decemlineata [Say]) in the north Xinjiang Uygur autonomous region in China has evolved resistance to pyrethroids and carbamates. Mutations resulting in target site insensitivity, namely L1014F in LdVssc1 voltage-sensitive sodium channel and S291G in acetylcholine esterase, confer, at least partially, the resistance. Insecticides with different modes of action may serve as likely replacements. Endosulfan and fipronil are GABA-gated chloride channel-blocking insecticides. In this article, we found that the contact toxicities of the two compounds were among the highest ever estimated to the 4th-instar larvae and the adults and affirmed that they were useful for L. decemlineata control in north Xinjiang. Regarding stomach toxicities of the two compounds, adult beetles were less sensitive than 2nd-, 3rd-, and 4th-instar larvae, suggesting that the appropriate timing for spraying is the early larval stage. Mixtures of endosulfan and α-cypermethrin at 1:160, 1:80, and 1:40 ratios, of endosulfan and phoxim at 1:24 ratio, and of endosulfan and isocarbophos at 1:72 and 1:288 ratios, significantly increased toxicity in a field population. The combination indices were significantly below 1 at both LD50 and LD90 levels, revealing synergistic effects. Our results demonstrated that endosulfan and fipronil could be applied alone and endosulfan may also be used in binary mixtures to restore pyrethroid susceptibility. These findings may have considerable practical implications for L. decemlineata resistance management. more...
- Published
- 2012
- Full Text
- View/download PDF
26. Chlorantraniliprole Susceptibility in Leptinotarsa decemlineata in the North Xinjiang Uygur Autonomous Region in China
- Author
-
Wen-Jun Fu, Wei-Ping Lu, Wei-Hua Jiang, Zhen-Han Xia, Guo-Qing Li, and Wen-Chao Guo
- Subjects
China ,Insecticides ,Piperonyl butoxide ,animal structures ,Piperonyl Butoxide ,Anthranilic diamide ,Insect Control ,Insecticide Resistance ,Toxicology ,chemistry.chemical_compound ,Cytochrome P-450 Enzyme System ,Animals ,ortho-Aminobenzoates ,Monooxygenase activity ,Leptinotarsa ,Glutathione Transferase ,Larva ,Ecology ,biology ,Potential risk ,fungi ,Colorado potato beetle ,Esterases ,Maleates ,General Medicine ,Pesticide ,biology.organism_classification ,Organophosphates ,Coleoptera ,chemistry ,Insect Science ,Insect Proteins - Abstract
The Colorado potato beetle (Leptinotarsa decemlineata (Say)) in the north Xinjiang Uygur autonomous region has evolved resistance to various types of insecticides. Chlorantraniliprole is a novel anthranilic diamide insecticide that binds and activates ryanodine receptors. It exhibited excellent efficacy against L. decemlineata in several field trails in Europe. In the present paper, the susceptibility of L. decemlineata fourth-instar larvae derived from six field populations and L. decemlineata adults derived from three field populations to chlorantraniliprole was determined by a topical application. The fourth-instar larvae were substantially more susceptible to chlorantraniliprole than adults, although the range of susceptibility was far greater among the fourth-instar larvae. Regarding stomach toxicities, adult beetles were less susceptible to chlorantraniliprole than larvae. Chlorantraniliprole was most toxic to second-instar larvae, followed by third- and fourth-instar larvae. These data suggested that the appropriate timing for chlorantraniliprole spraying is the early larval stage. Moreover, the synergistic activities of chlorantraniliprole in combination with triphenyl phosphate, diethyl maleate, or piperonyl butoxide against fourth-instar larvae from two field populations and adults from one field population were tested. Piperonyl butoxide had synergistic effects with chlorantraniliprole against fourth-instar larvae but not against adult beetles. Conversely, triphenyl phosphate and diethyl maleate exerted little synergistic effects. It appears that there is a potential risk of resistance against chlorantraniliprole resulting from cytochrome P450 monooxygenase activity. more...
- Published
- 2012
- Full Text
- View/download PDF
27. Target site insensitivity mutations in the AChE and LdVssc1 confer resistance to pyrethroids and carbamates in Leptinotarsa decemlineata in northern Xinjiang Uygur autonomous region
- Author
-
Man-Hui Xiong, Wei-Ping Lu, Guo-Qing Li, Xiao-Qin Shi, Wei-Hua Jiang, Zhi-Tian Wang, and Wen-Chao Guo
- Subjects
Veterinary medicine ,Piperonyl butoxide ,education.field_of_study ,Pyrethroid ,Health, Toxicology and Mutagenesis ,Population ,General Medicine ,Biology ,Pesticide ,Cyhalothrin ,chemistry.chemical_compound ,Deltamethrin ,chemistry ,parasitic diseases ,Botany ,Carbosulfan ,education ,Agronomy and Crop Science ,Carbofuran - Abstract
A survey of resistance to five conventional insecticides was conducted in 2009 and 2010 for the first generation 4th-instar larvae of Leptinotarsa decemlineata from Urumqi, Changji, Qitai and Qapqal. Compared with the Tekes population, a reference susceptible population, the Changji and Qapqal populations exhibited very high to moderate levels of resistance to cyhalothrin and deltamethrin, moderate to high levels of resistance to carbosulfan and carbofuran, and low levels of resistance to azinphosmethyl. Moreover, the Urumqi and the Qitai populations reached a high and a moderate level of resistance to carbosulfan, respectively. Synergistic effects of triphenyl phosphate, diethylmeleate, and piperonyl butoxide on cyhalothrin and carbosulfan in Changji population revealed that cytochrome P450s were involved in the resistance to cyhalothrin but not carbosulfan. A modified bi-PASA was developed to simultaneously detect point mutations of S291G in the AChE and L1014F in the LdVssc1 genes. The former mutation resulted in the resistance to carbamates and the latter in the resistance to pyrethroids. The rates of homozygous and heterozygous resistant individuals to carbamates (S291G mutation) were 17.6% and 14.7%, 50.6% and 42.2%, 49.9% and 41.7%, 51.3% and 41.4%, and 44.8% and 47.4%; to pyrethroids (L1014F mutation) were 5.8% and 8.7%, 36.1% and 27.0%, 41.8% and 24.8%, 12.2% and 9.7%, and 7.9% and 10.6%, respectively, in samples from Tekes, Changji, Qapqal, Urumqi and Qitai. I392T point mutation in the AChE was detected by RT-PCR among 18 individuals from Changji, Qapqal, Urumqi and Qitai. These results demonstrated that point mutations of S291G in the AChE and L1014F in the LdVscc1 are responsible for, at least partially, the resistance to carbamates and pyrethroids in L. decemlineata in some field populations in northern Xinjiang Uygur autonomous region. more...
- Published
- 2011
- Full Text
- View/download PDF
28. Susceptibilities of Leptinotarsa decemlineata (Say) in the North Xinjiang Uygur Autonomous Region in China to Two Biopesticides and Three Conventional Insecticides
- Author
-
Zhen-Han Xia, Guo-Qing Li, Wei-Hua Jiang, Xiao-Qin Shi, Wen-Jun Fu, Wen-Chao Guo, and Wei-Ping Lu
- Subjects
Larva ,Pyrethroid ,Colorado potato beetle ,Spinosad ,Biology ,biology.organism_classification ,Toxicology ,chemistry.chemical_compound ,Biopesticide ,chemistry ,Agronomy ,Insect Science ,Abamectin ,medicine ,Instar ,Leptinotarsa ,medicine.drug - Abstract
J. Agric. Urban Entomol. 27: 61-73 (2010/2011) ABSTRACT The Colorado potato beetle Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) in the northern part of the Xinjiang Uygur Autonomous Region has evolved resistance to several pyrethroid and carbamate insecticides. Biological control methods should be a major component of integrated pest management for L. decemlineata. Spinosad and abamectin are two biopesticides that have unique mechanisms of action. In this study, the contact toxicities of spinosad and abamectin to L. decemlineata fourth instars and adults were determined by topical applica- tions to several field populations. The average LD50 values of spinosad and abamectin for adults were 0.1275 and 0.0101 mg (a.i.) per individual, and for fourth instars they were 0.0181 and 0.0016 mg (a.i.) per individual, respectively. These data were among the lowest LD50s ever estimated, which affirmed that the two biopesticides are useful for L. decemlineata control in north Xinjiang. Susceptibilities to the two biopesticides varied slightly but significantly among tested field populations and the variations did not result from cross-resistance to conventional insecticides. Regarding stomach toxici- ties, the LC50 values of spinosad applied to excised potato leaves for second instars, third instars, fourth instars, and adults were 0.2840, 0.4093, 1.2413, and 2.3783 mg/L (a.i.), respectively. The LC50 values of abamectin for second instars, third instars, fourth instars, and adults were 0.0036, 0.0088, 0.0177, and 0.2591 mg/L (a.i.), respectively. The two biopesticides were most toxic to second instars, followed by third, then fourth instars, and they were least toxic to adults. These data suggested that the appropriate timing for spinosad or abamectin spraying is to early larval stages. more...
- Published
- 2010
- Full Text
- View/download PDF
29. Effects of different-intensity laser acupuncture at two adjacent same-meridian acupoints on nitric oxide and soluble guanylate cyclase releases in human
- Author
-
Jiang, Wan-Ling, primary, Wei, Hua-Jiang, additional, Guo, Zhou-Yi, additional, Ni, Yi-Rong, additional, Yang, Hong-Qin, additional, and Xie, Shu-Sen, additional
- Published
- 2017
- Full Text
- View/download PDF
30. The subunit gene Ldα1 of nicotinic acetylcholine receptors plays important roles in the toxicity of imidacloprid and thiamethoxam against Leptinotarsa decemlineata
- Author
-
Qiang Wang, Chenge Li, Wei-Hua Jiang, Jinhua Chen, Zhaojun Han, Wenchao Guo, and Yang Qu
- Subjects
0106 biological sciences ,0301 basic medicine ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Protein subunit ,Molecular Sequence Data ,Insect ,Receptors, Nicotinic ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Neonicotinoids ,Imidacloprid ,Botany ,Oxazines ,Bioassay ,Animals ,Amino Acid Sequence ,Leptinotarsa ,Phylogeny ,media_common ,Acetylcholine receptor ,biology ,Sequence Homology, Amino Acid ,fungi ,Imidazoles ,General Medicine ,biology.organism_classification ,Nitro Compounds ,Coleoptera ,010602 entomology ,Thiazoles ,030104 developmental biology ,Nicotinic agonist ,Biochemistry ,chemistry ,Thiamethoxam ,Agronomy and Crop Science - Abstract
Nicotinic acetylcholine receptors (nAChRs) are pentameric ACh-gated ion channels. It is believed that nAChRs composed of different subunits may vary in their function and toxicological characteristics. Neonicotinoids are activators of nAChRs and important insecticides that are extensively used for crop protection and resistance has been developed by some pests. They are also major insecticides for the control of Leptinotarsa decemlineata, which is a destructive defoliator pest that invaded the Xinjiang region of China in the 1990s. However, little is known about the constitution or subunits of the target in this pest. In this study, the full-length cDNAs encoding four new nAChR subunits (named Ldα3, Ldα6, Ldα10, and Ldβ1) were cloned from L. decemlineata. These genes encode 822-, 753-, 672-, and 759-amino acid proteins, respectively, which share typical features of insect nAChRs subunits and closely resemble the corresponding subunits of the nAChRs from Tribolium castaneum. Temporal and spatial expression analyses showed that these genes, as well as the previously identified Ldα1, Ldα2, and Ldα8 genes, are widely expressed in all developmental stages, including eggs, larvae of various instars, pupae, and adults. All genes monitored were expressed at higher levels in the head than in the thorax and abdomen, except for Ldα10. Dietary ingestion of double-stranded RNA bacterially expressed for Ldα1 (dsLdα1) significantly reduced the mRNA level of Ldα1 in treated larvae and adults by 48.0% and 78.6%, respectively. Among the non-target genes, Ldα3, Ldα9, and Ldβ1 were significantly up-regulated in larvae. A toxicity bioassay showed that dsLdα1 treatment greatly decreased the sensitivity to imidacloprid and thiamethoxam in adults. The larval susceptibility to thiamethoxam but not to imidacloprid was also reduced because of the lower down-regulation of Ldα1. Thus, our results suggest that Ldα1 encodes a subunit of a functional nAChR that mediates the toxicity of imidacloprid and thiamethoxam against L. decemlineata and that the down-regulation of Ldα1 might be an important mechanism for resistance and/or tolerance of L. decemlineata to neonicotinoids. more...
- Published
- 2015
31. PALB2 mutations in breast cancer patients from a multi-ethnic region in northwest China
- Author
-
Yong Tao Li, Ming Shuai Zhang, Wei Hua Jiang, Xiao Wen Wang, Cheng Guang Zhang, Li Na Yi, Aisikaer Ayoufu, Jiang Hua Ou, and Fulati WuwaliKhan
- Subjects
Oncology ,Adult ,medicine.medical_specialty ,China ,Genetic testing ,PALB2 ,Breast Neoplasms ,Disease ,Bioinformatics ,medicine.disease_cause ,Germline mutation ,Breast cancer ,Asian People ,Internal medicine ,medicine ,Missense mutation ,Humans ,Genetic Predisposition to Disease ,Family history ,Aged ,Mutation ,medicine.diagnostic_test ,business.industry ,Tumor Suppressor Proteins ,Research ,Nuclear Proteins ,General Medicine ,Middle Aged ,medicine.disease ,Female ,business ,Fanconi Anemia Complementation Group N Protein - Abstract
Background Germline mutations in PALB2 gene make a small contribution to heritable breast cancer susceptibility. A recent report has revealed that women with mutations in the PALB2 gene were more than nine times as likely to develop breast cancer compared to those without. The aim of this study is to understand the status of PALB2 mutations among Chinese high-risk breast cancer patients in a multi-ethnic region in China. Methods 152 patients with hereditary predisposition to breast cancer from the Xinjing region of China were enrolled in the study, and 100 control samples from healthy women were collected in the same locality. We sequenced the coding sequences and flanking intronic regions of PALB2 gene from DNA samples obtained from all subjects by direct sequencing. Results A total of 4 deleterious PALB2 mutations were identified in 152 breast cancer patients with a prevalence of about 2.6 % (4/152). The PALB2 mutation prevalence was 3.2 % (3/95) in cases with family history of breast cancer. In addition to the four deleterious mutations, we identified nine missense variants in 12 patients, using the prediction Softwares SIFT and PolyPhen, four of which might be disease associated (in 5 patients). Two of the 4 patients with deleterious mutations and 2 of the 5 patients presenting putative deleterious missense mutations had triple-negative breast cancer. No PALB2 mutation carriers were identified in 100 healthy controls. Conclusion PALB2 mutations account for a small, but not negligible, proportion of patients with hereditary predisposition to breast cancer in the Xinjing region of China. more...
- Published
- 2015
32. Changes in Nitric Oxide Releases of the Contralateral Acupoint during and after Laser Acupuncture at Bilateral Same-Name Acupoints in Human
- Author
-
Jiang, Wan-Ling, primary, Wei, Hua-Jiang, additional, Guo, Zhou-Yi, additional, Ni, Yi-Rong, additional, Yang, Hong-Qin, additional, and Xie, Shu-Sen, additional
- Published
- 2017
- Full Text
- View/download PDF
33. [Family-based association study of a variable number of tandem repeat polymorphism of DAT1 gene with Tourette syndrome in a Chinese Han population]
- Author
-
Lanlan, Zheng, Zhen-liang, Han, Xin-hua, Zhang, Xue-qin, Wang, Wei-hua, Jiang, Ming-ji, Yi, and Shi-guo, Liu
- Subjects
Adult ,Male ,Dopamine Plasma Membrane Transport Proteins ,Polymorphism, Genetic ,Adolescent ,Minisatellite Repeats ,Pedigree ,Young Adult ,Asian People ,Child, Preschool ,Humans ,Female ,Child ,Tourette Syndrome - Abstract
To assess the association of a 40 bp variable number of tandem repeat (VNTR) polymorphism within 3 untranslated region of dopamine transporter gene (DAT1) with Tourette syndrome (TS) in a Chinese Han population.A total of 160 TS patients and their parents were recruited. The VNTR polymorphism was detected with polymerase chain reaction-VNTR analysis, and its association with TS and its subtypes were assessed through a family-based association study comprising transmission disequilibrium test (TDT) and haplotype relative risk (HRR) analysis.The repeat numbers at the DAT1 40 bp locus were 11, 10, 9, 7.5 and 7 among the patients and their parents, with the most common type being a 10-repeat allele. No significant association was detected between the polymorphism and TS (TDT: X ² = 0.472, df = 1, P = 0.583; HRR: X ² = 0.313, P = 0.576, OR = 0.855, 95%CI: 0.493-1.481).Our data suggested that the VNTR polymorphism of DAT1 gene is not associated with susceptibility to TS in Chinese Han population. However, our results are to be validated in larger sets of patients collected from other populations. more...
- Published
- 2013
34. Prognostic significance of circulating tumor cells in esophageal carcinoma: a meta-analysis
- Author
-
Guanglei Qiao, Wei-hua Jiang, Wei-Xiang Qi, Ying Chen, and Lijun Ma
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Pathology ,Subgroup analysis ,Gastroenterology ,OncoTargets and Therapy ,Metastasis ,esophageal carcinoma ,03 medical and health sciences ,0302 clinical medicine ,Circulating tumor cell ,Internal medicine ,Carcinoma ,medicine ,metastasis ,Pharmacology (medical) ,Prospective cohort study ,Original Research ,Asian ,business.industry ,Hazard ratio ,Odds ratio ,medicine.disease ,Confidence interval ,meta-analysis ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,prognosis ,CTCs ,business - Abstract
Guang-Lei Qiao,1 Wei-Xiang Qi,2 Wei-Hua Jiang,1 Ying Chen,1 Li-Jun Ma1 1Department of Oncology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 2Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Shanghai, People’s Republic of China Purpose: The prognostic significance of circulating tumor cells (CTCs) in esophageal carcinoma (EC) is controversial. We aim to assess its association with clinicopathological and prognostic relevance in EC by using a meta-analysis.Methods: We searched PubMed, Cochrane Database, Embase databases, and the references in relevant studies that assessed the clinicopathological or prognostic relevance of CTCs in peripheral blood of patients with EC. Statistical analyses were conducted by using Stata software to calculate the pooled odds ratio (OR), hazard ratio (HR), and 95% confidence intervals (CIs) using fixed or random-effects models according to the heterogeneity of included studies. The subgroup analyses were performed according to ethnicity, histological type, and detection method.Results:Sixteen trials containing 1,260 patients were included for analysis. Pooled results showed that presence of CTCs was significantly associated with poor overall survival (HR=1.71, 95% CI [1.30, 2.12],P more...
- Published
- 2016
- Full Text
- View/download PDF
35. Insecticide resistance status of Colorado potato beetle (Coleoptera: Chrysomelidae) adults in northern Xinjiang Uygur autonomous region
- Author
-
Guo-Qing Li, Ping Liu, Wen-Chao Guo, Man-Hui Xiong, Zhi-Tian Wang, Wei-Ping Lu, and Wei-Hua Jiang
- Subjects
Veterinary medicine ,education.field_of_study ,Piperonyl butoxide ,China ,Insecticides ,Pesticide resistance ,Ecology ,Population ,Colorado potato beetle ,General Medicine ,Biology ,biology.organism_classification ,Cyhalothrin ,Coleoptera ,Insecticide Resistance ,chemistry.chemical_compound ,Deltamethrin ,chemistry ,Agronomy ,Insect Science ,Animals ,education ,Carbofuran ,Endosulfan - Abstract
Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), has become the economically most important insect defoliator of potatoes, Solanum tuberosum L., in northern Xinjiang Uygur autonomous region in China. Currently, control of Colorado potato beetle relies mainly on chemical insecticides. And this may result in insecticide resistance. In this study, LD50 values were measured by a topical bioassay for 14 conventional insecticides in seven local populations from Urumqi, Changji, Tacheng, Nilka, Gongliu, Qapqal, and Tekes counties (cities). The Tekes field population was the most susceptible population and was selected as a reference strain. Compared with the Tekes strain, the Changji, Qapqal, Nilka, Tacheng, and Gongliu populations exhibited moderate to very high levels of resistance to cyhalothrin. The Qapqal and Changji populations showed a moderate and a very high resistance to deltamethrin, respectively. And the Changji population developed a high resistance against alpha-cypermethrin. Moreover, the Qapqal population had a moderate resistance to carbofuran, and the Urumqi population reached high level of resistance to endosulfan. Possible resistance mechanisms of the Changji and Qapqal populations were determined using three enzyme inhibitors. Triphenyl phosphate (TPP), diethylmeleate, and piperonyl butoxide (PBO) had little synergism to cyhalothrin in the two populations. In contrast, PBO and TPP exhibited some synergistic effects to carbofuran in the Qapqal population, indicating the involvement of monooxygenases and esterases in conferring carbofuran resistance. It seems that additional mechanisms, such as target site insensitivity, should play an important role in Colorado potato beetle resistances to cyhalothrin and carbofuran in northern Xinjiang local populations. more...
- Published
- 2010
36. [The investigation of the relationship between the phenotypes of 46, XX males and the SRY gene]
- Author
-
Yue-ping, Wang, Yun-jing, Zhao, Qiang, Ruan, Yao-hua, Ji, Ming-ge, Hao, and Wei-hua, Jiang
- Subjects
Adult ,Male ,Phenotype ,Child, Preschool ,Humans ,Infant ,Sex Chromosome Disorders ,Sequence Analysis, DNA ,Genes, sry ,Genitalia, Male ,Polymerase Chain Reaction ,Sex Chromosome Aberrations - Abstract
To investigate the relationship between the phenotypes in XX male patients and the sex determining region(SRY) gene.Multiple polymerase chain reactions were carried out in 6 male patients with karyotype of 46, XX, and then the PCR products were sequenced directly.Three cases of male infertility were positive for the SRY gene without evident malformation in their extra genitalia, while 3 cases with testes were negative for the SRY gene, with evident malformation in their extra genitalia.The SRY gene is key in sex determination and development, yet there might be other important genes involved. more...
- Published
- 2009
37. ChemInform Abstract: A Simple, Efficient Pd-Catalyzed Synthesis of N-Sulfonylimines from Organoboronic Acids and Tosylbenzimidoyl Chlorides
- Author
-
Feifeng Gao, Wei-Hua Jiang, Changtao Qian, Min-Zhi Deng, and Li-Yan Fan
- Subjects
Chemistry ,Simple (abstract algebra) ,General Medicine ,Combinatorial chemistry ,Catalysis - Abstract
A simple and efficient synthesis of N-sulfonyl ketimines through a Pd-catalyzed cross-coupling reaction between organoboronic acids and tosylbenzimidoyl chlorides under mild conditions has been developed. more...
- Published
- 2008
- Full Text
- View/download PDF
38. A simple, efficient Pd-catalyzed synthesis of N-sulfonylimines from organoboronic acids and tosylbenzimidoyl chlorides
- Author
-
Feifeng Gao, Li-Yan Fan, Changtao Qian, Wei-Hua Jiang, and Min-Zhi Deng
- Subjects
Chemistry ,Simple (abstract algebra) ,Organic Chemistry ,Physical and Theoretical Chemistry ,Biochemistry ,Combinatorial chemistry ,Catalysis - Abstract
A simple and efficient synthesis of N-sulfonyl ketimines through a Pd-catalyzed cross-coupling reaction between organoboronic acids and tosylbenzimidoyl chlorides under mild conditions has been developed. more...
- Published
- 2008
39. Constructing GKMS-Based E-Learning Community
- Author
-
Wei-Hua Jiang, Ya-Ya Chen, and Hong-Wei Luo
- Subjects
Resource (project management) ,Knowledge management ,Computer science ,business.industry ,E-learning (theory) ,Management system ,Distance education ,Women's studies ,The Internet ,General knowledge ,business ,Data science - Abstract
Combining the ideas of general knowledge management system and the technology of World Wide Web, a perfect women's studies knowledge system can be established. Thus, by choosing, classifying and managing correlative knowledge, online academic resource for women's studies are able to be accumulated, spread and shared, by which women's studies e-learning community can be rapidly constructed. more...
- Published
- 2007
- Full Text
- View/download PDF
40. Prognostic significance of circulating tumor cells in esophageal carcinoma: a meta-analysis.
- Author
-
Guang-Lei Qiao, Wei-Xiang Qi, Wei-Hua Jiang, Ying Chen, and Li-Jun Ma
- Subjects
ESOPHAGEAL cancer ,CANCER cells ,CANCER patients ,POLYMERASE chain reaction ,SQUAMOUS cell carcinoma ,CANCER prognosis - Abstract
Purpose: The prognostic significance of circulating tumor cells (CTCs) in esophageal carcinoma (EC) is controversial. We aim to assess its association with clinicopathological and prognostic relevance in EC by using a meta-analysis. Methods: We searched PubMed, Cochrane Database, Embase databases, and the references in relevant studies that assessed the clinicopathological or prognostic relevance of CTCs in peripheral blood of patients with EC. Statistical analyses were conducted by using Stata software to calculate the pooled odds ratio (OR), hazard ratio (HR), and 95% confidence intervals (CIs) using fixed or random-effects models according to the heterogeneity of included studies. The subgroup analyses were performed according to ethnicity, histological type, and detection method. Results: Sixteen trials containing 1,260 patients were included for analysis. Pooled results showed that presence of CTCs was significantly associated with poor overall survival (HR =1.71, 95% CI [1.30, 2.12], P<0.001) and progression-free survival (HR =1.67, 95% CI [1.19, 2.15], P<0.001) in EC patients. Subgroup analysis indicated that presence of CTCs was closely associated with worse overall survival (Asian: HR =1.66, 95% CI [1.24, 2.08], P<0.001; squamous cell carcinoma [SCC]: HR =1.66, 95% CI [1.24, 2.08], P<0.001; no polymerase chain reaction [PCR]: HR =2.08, 95% CI [1.40, 2.76], P<0.001) and progression-free survival (Asian: HR =1.63, 95% CI [1.15, 2.12], P<0.001; SCC: HR =1.63, 95% CI [1.15, 2.12], P<0.001; PCR: HR =1.63, 95% CI [1.15, 2.12], P<0.001). Additionally, ORs showed that presence of CTCs was significantly correlated with tumor node metastasis (TNM) staging (overall: OR = 1.96, 95% CI [1.34, 2.87], P=0.001; Asian: OR =2.09, 95% CI [1.37, 3.19], P=0.001; SCC: OR =1.97, 95% CI [1.21, 3.07], P=0.003; PCR: OR =2.23, 95% CI [1.43, 3.47], P<0.001), venous invasion (overall: OR =2.23, 95% CI [1.46, 3.40], P<0.001; Asian: OR =2.23, 95% CI [1.46, 3.40], P<0.001; SCC: OR =2.23, 95% CI [1.46, 3.40], P<0.001; PCR: OR =2.23, 95% CI [1.46, 3.40], P<0.001), lymph node metastasis (overall: OR =2.41, 95% CI [1.50, 3.86], P<0.001; Asian: OR =2.89, 95% CI [1.80, 4.65], P<0.001; SCC: OR =2.44, 95% CI [1.47, 4.07], P=0.001; PCR: OR =2.89, 95% CI [1.80, 4.65], P<0.001) and distant metastasis (Asian: OR =2.68, 95% CI [1.01, 7.08], P=0.047) in patients with EC. Conclusion: The presence of CTCs indicates a poor prognosis in EC patients, especially in Asian and SCC patients. Further well-designed prospective studies are recommended to explore the clinical applications of CTCs in patients with EC. [ABSTRACT FROM AUTHOR] more...
- Published
- 2016
- Full Text
- View/download PDF
41. Using an oblique incident laser beam to measure the optical properties of stomach mucosa/submucosa tissue
- Author
-
Wei, Hua Jiang, primary, Xing, Da, additional, He, Bo Hua, additional, Gu, Huai Min, additional, Wu, Guo Yong, additional, and Chen, Xue Mei, additional
- Published
- 2009
- Full Text
- View/download PDF
42. Novel High-Throughput Profiling of Human Transcription Factors and Its Use for Systematic Pathway Mapping
- Author
-
Qiao, Ji-Ying, primary, Shao, Wei, additional, Wei, Hua-Jiang, additional, Sun, Yi-Min, additional, Zhao, Yong-Chao, additional, Xing, Wan-Li, additional, Zhang, Liang, additional, Mitchelson, Keith, additional, and Cheng, Jing, additional more...
- Published
- 2008
- Full Text
- View/download PDF
43. Properties of autofluorescence, absorption coefficient and scattering coefficient spectra for human gastric adenocarcinoma tissues
- Author
-
He, Bo-Hua, primary, Wei, Hua-Jiang, additional, Chen, Xue-Mei, additional, and Wang, Juan, additional
- Published
- 2008
- Full Text
- View/download PDF
44. Parallel Profiling of Active Transcription Factors Using an Oligonucleotide Array-Based Transcription Factor Assay (OATFA)
- Author
-
Shao, Wei, primary, Wei, Hua-Jiang, additional, Qiao, Ji-Ying, additional, Zhao, Yong-Chao, additional, Sun, Yi-Min, additional, Zhou, Yu-Xiang, additional, and Cheng, Jing, additional
- Published
- 2005
- Full Text
- View/download PDF
45. Determination of optical properties of normal and adenomatous human colon tissuesin vitrousing integrating sphere techniques
- Author
-
Wei, Hua-Jiang, primary
- Published
- 2005
- Full Text
- View/download PDF
46. Differences in optical properties between healthy and pathological human colon tissues using a Ti:sapphire laser: an in vitro study using the Monte Carlo inversion technique
- Author
-
Wei, Hua-Jiang, primary, Xing, Da, additional, Wu, Guo-Yong, additional, Gu, Huai-Min, additional, Lu, Jian-Jun, additional, Jin, Ying, additional, and Li, Xiao-Yuan, additional
- Published
- 2005
- Full Text
- View/download PDF
47. Optical properties of human normal small intestine tissue determined by Kubelka-Munk methodin vitro
- Author
-
Wei, Hua-Jiang, primary
- Published
- 2003
- Full Text
- View/download PDF
48. Chlorantraniliprole Susceptibility in Leptinotarsa decemlineata in the North Xinjiang Uygur Autonomous Region in China.
- Author
-
Wei-Hua Jiang, Wei-Ping Lu, Wen-Chao Guo, Zhen-Han Xia, Wen-Jun Fu, and Guo-Qing Li
- Subjects
COLORADO potato beetle ,CHLORANTRANILIPROLE ,TOXICITY testing ,INSECTICIDES ,RYANODINE receptors - Abstract
The Colorado potato beetle (Leptinotarsa decemlineata (Say)) in the north Xinjiang Uygur autonomous region has evolved resistance to various types of insecticides. Chlorantraniliprole is a novel anthranilic diamide insecticide that binds and activates ryanodine receptors. It exhibited excellent efficacy against L. decemlineata in several field trails in Europe. In the present paper, the susceptibility of L. decemlineata fourth-instar larvae derived from six field populations and L. decemlineata adults derived from three field populations to chlorantraniliprole was determined by a topical application. The fourth-instar larvae were substantially more susceptible to chlorantraniliprole than adults, although the range of susceptibility was far greater among the fourth-instar larvae. Regarding stomach toxicities, adult beetles were less susceptible to chlorantraniliprole than larvae. Chlorantraniliprole was most toxic to second-instar larvae, followed by third- and fourth-instar larvae. These data suggested that the appropriate timing for chlorantraniliprole spraying is the early larval stage. Moreover, the synergistic activities of chlorantraniliprole in combination with triphenyl phosphate, diethyl maleate, or piperonyl butoxide against fourth-instar larvae from two field populations and adults from one field population were tested. Piperonyl butoxide had synergistic effects with chlorantraniliprole against fourth-instar larvae but not against adult beetles. Conversely, triphenyl phosphate and diethyl maleate exerted little synergistic effects. It appears that there is a potential risk of resistance against chlorantraniliprole resulting from cytochrome P450 monooxygenase activity. [ABSTRACT FROM AUTHOR] more...
- Published
- 2012
- Full Text
- View/download PDF
49. Insecticide Resistance Status of Colorado Potato Beetle (Coleoptera: Chrysomelidae) Adults in Northern Xinjiang Uygur Autonomous Region.
- Author
-
Wei-Hua Jiang, Zhi-Tian Wang, Man-Hui Xiong, Wei-Ping Lu, Ping Liu, Wen-Chao Guo, and Guo-Qing Li
- Subjects
COLORADO potato beetle ,INSECTICIDE resistance ,CARBOFURAN ,BIOLOGICAL assay ,MONOOXYGENASES ,ESTERASES - Abstract
Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), has become the economically most important insect defoliator of potatoes, Solanum tuberosum L., in northern Xinjiang Uygur autonomous region in China. Currently, control of Colorado potato beetle relies mainly on chemical insecticides. And this may result in insecticide resistance. In this study, LDm values were measured by a topical bioassay for 14 conventional insecticides in seven local populations from Urumqi, Changji, Tacheng, Nilka, Gongliu, Qapqal, and Tekes counties (cities). The Tekes field population was the most susceptible population and was selected as a reference strain. Compared with the Tekes strain, the Changji, Qapqal, Nilka, Tacheng, and Congliu populations exhibited moderate to very high levels of resistance to cyhalothrin. The Qapqal and Changji populations showed a moderate and a very high resistance to deltarnethrin, respectively. And the Changji population developed a high resistance against a-cypermethrin. Moreover, the Qapqal population had a moderate resistance to carbofuran, and the Urumqi population reached high level of resistance to endosulfan. Possible resistance mechanisms of the Changji and Qapqal populations were determined using three enzyme inhibitors. Triphenyl phosphate (TPP), diethylmeleate, and piperonyl butoxide (PBO) had little synergism to cyhalothrin in the two populations. In contrast, PBO and TPP exhibited some synergistic effects to carbofuran in the Qapqal population, indicating the involvement of monooxygenases and esterases in conferring carbofuran resistance. It seems that additional mechanisms, such as target site insensitivity, should play an important role in Colorado potato beetle resistances to cyhalothrin and carbofuran in northern Xinjiang local populations. [ABSTRACT FROM AUTHOR] more...
- Published
- 2010
- Full Text
- View/download PDF
50. Solvability of 2 n-order m-point boundary value problem at resonance.
- Author
-
Wei-hua Jiang, Yan-ping Guo, and Ji-qing Qiu
- Subjects
- *
BOUNDARY value problems , *DIFFERENTIAL equations , *NUMERICAL solutions to Fredholm equations , *NONLINEAR boundary value problems , *COMPLEX variables , *RESONANCE - Abstract
The existence of solutions for the 2n-order m-point boundary value problem at resonance is obtained by using the coincidence degree theory of Mawhin. We give an example to demonstrate our result. The interest is that the nonlinear term may be noncontinuous. [ABSTRACT FROM AUTHOR] more...
- Published
- 2007
- Full Text
- View/download PDF
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.