181 results on '"Wei Ting Lee"'
Search Results
2. TORC1 regulates the transcriptional response to glucose and developmental cycle via the Tap42-Sit4-Rrd1/2 pathway in Saccharomyces cerevisiae
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Mohammad Alfatah, Jin Huei Wong, Vidhya Gomathi Krishnan, Yong Cheow Lee, Quan Feng Sin, Corinna Jie Hui Goh, Kiat Whye Kong, Wei Ting Lee, Jacqueline Lewis, Shawn Hoon, and Prakash Arumugam
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Transcriptional response to glucose ,TORC1 ,Tap42/Sit4/Rrd1-2 module ,Spore germination ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Target of Rapamycin Complex 1 (TORC1) is a highly conserved eukaryotic protein complex that couples the presence of growth factors and nutrients in the environment with cellular proliferation. TORC1 is primarily implicated in linking amino acid levels with cellular growth in yeast and mammals. Although glucose deprivation has been shown to cause TORC1 inactivation in yeast, the precise role of TORC1 in glucose signaling and the underlying mechanisms remain unclear. Results We demonstrate that the presence of glucose in the growth medium is both necessary and sufficient for TORC1 activation. TORC1 activity increases upon addition of glucose to yeast cells growing in a non-fermentable carbon source. Conversely, shifting yeast cells from glucose to a non-fermentable carbon source reduces TORC1 activity. Analysis of transcriptomic data revealed that glucose and TORC1 co-regulate about 27% (1668/6004) of yeast genes. We demonstrate that TORC1 orchestrates the expression of glucose-responsive genes mainly via the Tap42-Sit4-Rrd1/2 pathway. To confirm TORC1’s function in glucose signaling, we tested its role in spore germination, a glucose-dependent developmental state transition in yeast. TORC1 regulates the glucose-responsive genes during spore germination and inhibition of TORC1 blocks spore germination. Conclusions Our studies indicate that a regulatory loop that involves activation of TORC1 by glucose and regulation of glucose-responsive genes by TORC1, mediates nutritional control of growth and development in yeast.
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- 2021
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3. Instability of the Medial Column Post Triple Arthrodesis for Stage III Posterior Tibial Tendon Dysfunction
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Wei Ting Lee MBBS, MRCS(Ed), MMed(Ortho), FRCS(Ed), FAMS, Adriana Avila MD, Qin Ling Han, and Mark S. Myerson MD
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Orthopedic surgery ,RD701-811 - Abstract
Category: Hindfoot Introduction/Purpose: Triple arthrodesis remains the ideal procedure for management of painful rigid flatfoot deformity. While concurrent extended medial column arthrodesis of either the cuneonavicular joint or the 1st tarsometatarsal joint is occasionally required, they are not routinely performed. We hypothesized that following triple arthrodesis, there will be minimal change in the alignment of the medial column joints. The aim of this study was to examine the effect of the triple arthrodesis on the stability and alignment of the medial column joints. Methods: This was a retrospective review of 212 consecutive patients who underwent triple arthrodesis between 2002-2013 for correction of painful Stage III flatfoot deformity. Radiographs of the feet were obtained preoperatively, at 6 weeks, 6 months, 1 year, 3 years and 5 years following surgery. The post operative stability and alignment of the medial column was determined by comparing the radiographs over time. Five radiological parameters were studied: medial cuneiform-calcaneus angle (CCA), medial cuneiform-1st metatarsal angle (MCA), 1st/2nd inter metatarsal angle (IMA), medial cuneiform-navicular distance (NCD) and calcaneal pitch angle. These measurements determined the presence of instability in the sagittal plane (CCA and MCA) and coronal plane (NCD and IMA) of the medial cuneonavicular (NC) joint, and the 1st tarsometatarsal(TMT) joint. Results: Our study demonstrated the presence of medial column instability after triple arthrodesis. This instability was most apparent in the sagittal plane of the NC joint (P < 0.001), and was associated with a decreased in the calcaneal pitch (P < 0.001). There was a slight decrease in the IMA(P < 0.001) as well. The coronal plane of the NC joint, and the sagittal plane of the 1st TMT joint remained stable throughout the measurements. The instability is best appreciated in Fig(B) where there is a reduction in the CCA and calcaneal pitch compared to the X-ray in Fig(A) taken 15 months earlier. Conclusion: The triple arthrodesis procedure corrects the foot deformity, but it is associated with subsequent instability of the medial column mild at the NC joint and abduction of the forefoot at the TMT joints. However, the clinical significance of this instability is not apparent from the study. Since very few patients required subsequent treatment for TMT and NC pathologies after a successful triple arthrodesis, we found insufficient justification for extending the triple arthrodesis distally.
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- 2016
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4. What Variables Influence Final Range of Motion Following Total Ankle Arthroplasty
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Wei Ting Lee MBBS, MRCS(Ed), MMed(Ortho), FRCS(Ed), FAMS, Mark S. Myerson MD, and Kevin T. Grosshans MD
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Orthopedic surgery ,RD701-811 - Abstract
Category: Ankle Arthritis Introduction/Purpose: While studies have demonstrated a predictable incremental increase in the range of motion (ROM) following total ankle arthroplasty (TAA) it remains unclear what variables affect the post-operative ROM. This study was undertaken to further investigate this question with the goal of providing guidance for patients and surgeons deciding between TAA and arthrodesis. Methods: This is a prospective review of 223 consecutive patients who underwent primary TAA using a single prosthesis performed between 2010-2013 for painful ankle arthritis that failed conservative therapy. Weight bearing flexion and extension X- rays were obtained preoperatively and again at 1 year following surgery. The patients were compared for demographics, type of arthritis, preoperative malalignment, prior trauma, obesity, medical co-morbidities, and the effect of concurrent surgery on post operative ankle ROM. Results: 191 patients had adequate follow-up and met all the inclusion criteria. The preoperative diagnosis, hindfoot or ankle varus or valgus, and concurrent percutaneous tendon Achilles lengthening (TAL) procedures were positive predictors of greater improvement in ankle ROM after TAA. Patients with valgus ankle malalignment had better post TAA DF, PF and total ankle range of motion compared to those in varus (P < 0.05). Percutaneous tendon Achilles lengthening (TAL) improved the DF, but not the PF (P < 0.05). Patients with primary ankle osteoarthritis (n=105) had the best improvement in ROM with an average gain of 3.39° in dorsiflexion (DF), 2.47° in plantar flexion (PF) and 5.84° in total ankle ROM (P < 0.05). An analysis of this subgroup demonstrated greater gain in ankle ROM in patients with lower preoperative ROM compared to those with higher preoperative ankle ROM(P < 0.01). Conclusion: Our results indicated primary ankle OA, preoperative ankle valgus malalignment and intraoperative TAL are significant factors in predicting increased ankle ROM after TAA. Ultimately, ankle motion is a function of the extent of arthritis, fibrosis and the surrounding soft tissue envelope. While TAA restores the function of the joint, it may not adequately correct periarticular fibrosis. Early weight bearing and physical therapy may alleviate postoperative stiffness, and increase the post operative gain in ROM. Further study is underway to determine the effect of early weight bearing and initiation of physical therapy on the ROM following TAA.
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- 2016
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5. Short-Term Outcome after Computer-Assisted versus Conventional Total Knee Arthroplasty: A Randomised Controlled Trial
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Wei Ting Lee, Pak Lin Chin, Ngai Nung Lo, and Seng Jin Yeo
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Orthopedic surgery ,RD701-811 - Abstract
Purpose. To compare the short-term functional outcome after computer-assisted total knee arthroplasty (TKA) versus conventional TKA. Methods. 23 men and 67 women aged 48 to 80 years were randomised to undergo (1) conventional TKA using an intramedullary guide, (2) conventional TKA using an extramedullary guide, or (3) computer-assisted TKA. Two senior surgeons performed all the TKAs using the same TKA system and the standard anteromedial arthrotomy with eversion of the patella. Patients were assessed by physiotherapists before and 6 months and 2 years after TKA using the Short Form-36 Health Survey, Oxford Knee Score, and Knee Society Score. Results. Of the 90 patients, 67 and 70 were assessed at 6 months and 2 years after TKA, respectively. No patient developed deep infection or required revision surgery. Functional outcomes of the 3 groups did not differ significantly at the corresponding follow-ups. Conclusion. Significant improvement in the functional outcome was not shown in patients treated with computer-assisted TKA, compared with conventional TKA. Thus, computer-assisted TKA has limited additional short-term benefits. Further studies with longer follow-up are required to examine the benefits of computer-assisted TKA.
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- 2015
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6. Authors' Reply
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Wei Ting Lee
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Orthopedic surgery ,RD701-811 - Published
- 2015
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7. Proximal Femoral Locking Compression Plate for Proximal Femoral Fractures
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Wei Ting Lee, Diarmuid Murphy, Fareed HY Kagda, and Joseph Thambiah
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Orthopedic surgery ,RD701-811 - Abstract
Purpose. To review short-term outcomes of proximal femoral locking compression plate (PF-LCP) fixation for proximal femoral fractures in terms of postoperative complications and failure rates. Methods. Medical records of 21 men and 5 women aged 22 to 85 (mean, 49.7) years who underwent internal fixation with the PF-LCP for proximal femoral fractures were reviewed. Younger patients (mean age, 38.7 years) were more commonly involved in high-energy trauma with multiple musculoskeletal injuries, whereas older patients (mean age, 67.7 years) were more commonly involved in low-energy trauma. Fractures were classified into: multi-fragmentary pertrochanteric fractures (n=13), transtrochanteric fractures (n=6), and subtrochanteric/proximal diaphyseal fractures (n=7). Results. Patients were followed up for a mean of 14.7 months. Seven patients developed complications including loosening of locking screws (n=4), delayed union (n=2), and infection (n=1); 4 of them required additional surgeries. Conclusion. The PF-LCP is appropriate for complex proximal femoral fractures with poor bone quality, revision surgeries, and multi-fragmentary subtrochanteric/proximal diaphyseal fractures. For intertrochanteric fractures, the sliding hip screw system should be used to avoid failure.
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- 2014
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8. Treatment outcome and prognostic factors of external auditory canal squamous cell carcinoma: A retrospective study in a tertiary center
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Yi‐Ting Huang, Jiunn‐Liang Wu, Wen‐Yuan Chao, and Wei‐Ting Lee
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external auditory canal ,prognostic factor ,squamous cell carcinoma ,survival outcome ,temporal bone ,Otorhinolaryngology ,RF1-547 ,Surgery ,RD1-811 - Abstract
Abstract Objective Squamous cell carcinoma (SCC) of the external auditory canal (EAC) is a rare malignancy with various treatment strategies and outcomes. The purpose of this study was to evaluate the clinical characteristics and survival outcomes and identify prognostic factors in patients with SCC of EAC. Methods Twenty‐one patients with SCC of EAC treated in a single tertiary center between 2009 and 2021 were retrospectively reviewed and analyzed. The modified Pittsburgh classification system was applied for staging. Factors associated with survival were identified by univariate survival analysis. Results The mean age at diagnosis was 61 years (range: 41–79 years). Early‐stage (T1 + T2) accounts for 38.1% of the series and advanced‐stage (T3 + T4) accounts for 61.9%. Eighteen (85.7%) patients underwent primary surgery with curative intent. The 5‐year overall survival rate of the 21 patients was 67.4%. Tumor invasion to the otic capsule, eustachian tube, sigmoid sinus, and dura were associated with poor prognosis in univariate analysis (p = .046; .008; .027; and .08, respectively). Conclusions Factors predictive of less favorable survival include the history of COM, tumor invasion to the otic capsule, eustachian tube, sigmoid sinus, and dura. It is important to make a precise and systemic preoperative evaluation of disease extent. Level of Evidence 4
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- 2024
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9. Tissue Inhibitor of Metalloproteinase 3: Unravelling Its Biological Function and Significance in Oncology
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Wei-Ting Lee, Pei-Ying Wu, Ya-Min Cheng, and Yu-Fang Huang
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tissue inhibitor of metalloproteinases-3 ,gynaecological cancers ,biomarker ,cancer therapy ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Tissue inhibitor of metalloproteinases-3 (TIMP3) is vital in regulating several biological processes. TIMP3 exerts antitumour effects via matrix metalloproteinase (MMP)-dependent and MMP-independent pathways. Due to promoter methylation and miRNA binding, TIMP3 expression has been observed to decrease in various cancers. Consequently, the migration and invasion of cancer cells increases. Conflicting results have reported that expression levels of TIMP3 in primary and advanced cancers are higher than those in healthy tissues. Therefore, the role of TIMP3 in cancer biology and progression needs to be elucidated. This review provides an overview of TIMP3, from its biological function to its effects on various cancers. Moreover, gynaecological cancers are discussed in detail. TIMP3 has been associated with cervical adenocarcinoma as well as cancer development in serous ovarian cancer and breast cancer metastasis. However, the relationship between TIMP3 and endometrial cancers remains unclear. TIMP3 may be a useful biomarker for gynaecological cancers and is a potential target for future cancer therapy.
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- 2024
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10. Distinct Failure Patterns in Hypopharyngeal Cancer Patients Receiving Surgery-Based Versus Radiation-Based Treatment
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Yu-Hsuan Lin, Jenn-Ren Hsiao, Yuan-Hua Wu, Jeffrey S. Chang, Chun-Yen Ou, Wei-Ting Lee, Cheng-Chih Huang, Chan-Chi Chang, Yu-Hsuan Lai, Sen-Tien Tsai, Wei-Ting Hsueh, Chia-Jui Yen, Chen-Lin Lin, Yu-Shan Chen, Shih-Sheng Jiang, Yu-Chu Su, and Shang-Yin Wu
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Hypopharyngeal Neoplasms ,Oncology ,Humans ,Surgery ,Chemoradiotherapy ,Neoplasm Recurrence, Local ,Retrospective Studies ,Neoplasm Staging - Abstract
Background To cure advanced hypopharyngeal squamous cell carcinoma (HPSCC), primary operation followed by adjuvant (chemo-)radiotherapy (OP-CRT) or definitive chemoradiation (CCRT) are the two primary options. This study aimed to compare the failure patterns and long-term survival outcomes of HPSCC patients treated with these two strategies. Patients and Methods From 2007 to 2015, 198 pathologically confirmed HPSCC patients receiving either OP-CRT or CCRT were retrospectively reviewed. Failure patterns and survival outcomes stratified by the 7th American Joint Committee on Cancer staging system and treatment modalities were compared. Results One hundred and eighty-nine patients (95.4%) were stage III/IV and 62 patients (31.3%) received OP-CRT. Median follow-up duration was 4.9 years. Compared with CCRT, OP-CRT provided better 3-year local relapse-free survival for T3 (93 vs 48%, p < 0.0001), T4a (88 vs 37%, p = 0.0005) and better 3-year regional relapse-free survival for N2b+2c (93 vs 60%, p < 0.0001). Of note, for stage IVA subjects, OP-CRT provided better 3-year loco-regional relapse-free survival (85 vs 37%, p < 0.0001), marginal poor 3-year distant metastasis-free survival (62 vs 79%, p = 0.06), but comparable 3-year OS (52 vs 44%, p = 0.37) and 5-year OS (44 vs 31%, p = 0.15) compared with CCRT. Conclusions For patients with advanced HPSCC, although OP-CRT and CCRT provided similar overall survival, failure patterns were distinct. OP-CRT provided better loco-regional control but was more likely to encounter distant metastases than CCRT. The detailed analysis of failure patterns will pave the way to improve this devastating disease.
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- 2022
11. Clinical features in salivary gland lymphoepithelial carcinoma in 10 patients: Case series and literature review
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Chiung‐Tung Chou, Chun‐Yen Ou, Wei‐Ting Lee, and Heng‐Jui Hsu
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General Medicine - Abstract
Lymphoepithelial carcinoma (LEC) accounts for 0.4% of malignant tumors of the salivary gland and 0.8% of parotid gland malignancies. Over the past 50 years, less than 300 cases have been reported in the literature. The purpose of this study was to investigate the characteristics of salivary gland LEC.We retrospectively reviewed the medical records and analyzed clinical data obtained from 10 patients seen at our hospital between 2005 and 2020 with salivary gland LEC.All patients presented with a self-palpable, non-tender, hard swelling, or lump near the jaw or infra-auricular region. Most cases (Although rare and considered to be a high-risk malignancy, LECs have favorable treatment outcomes. Circulating EBV DNA is still not considered a marker for preoperative assessment or postoperative treatment response. The role of EBV DNA requires further investigation.Level of Evidence: 4.
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- 2022
12. Infinite speed drives control with MTPA and MTPV for interior permanent magnet synchronous motor.
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Ping-Yi Lin, Wei-Ting Lee, Shang-Wei Chen, Jonq-Chin Hwang, and Yen-Shin Lai
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- 2014
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13. The Additive Effectiveness of Inspiratory Muscle Training on Glottic Closure and Subjective Voice Outcomes of Patients With Benign Lesion After Hyaluronic Acid Laryngoplasty
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Hsin-Hao Liou, Miyuki Hsing-Chun Hsieh, David Shang-Yu Hung, Hui-Ling Liu, Ishan Lee, Yi-Chih Lin, Jenn-Ren Hsiao, Cheng-Chih Huang, Chun-Yen Ou, Chan-Chi Chang, Wei-Ting Lee, Sen-Tien Tsai, and Shu-Wei Tsai
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Speech and Hearing ,Otorhinolaryngology ,LPN and LVN - Abstract
For patients with glottic insufficiency disease, injection laryngoplasty is a rapid and efficient management option that complements voice therapy. Some studies have indicated that respiratory muscle training may also show promise in patients with voice disorders. However, the effect of respiratory muscle training in patients with glottic insufficiency was reported to be limited, and whether it provides additional benefit after standard management requires further evaluation. We aimed to investigate the effectiveness of inspiratory muscle training on glottis closure and patient-reported voice quality in glottic insufficiency patients who had been treated with hyaluronic acid injection.Retrospective observational study.We included 46 patients with glottic insufficiency who had undergone hyaluronic acid injection. Twenty of them had undergone inspiratory muscle training during three months. We measured patients' changes in glottic status according to the normalized glottal gap area and bowing index, as well as voice quality of life according to the voice handicap index 10 and the voice outcome survey, before and after training.Patients who underwent inspiratory muscle training had higher odds of experiencing better improvement in all scores. The range of odds ratios ranged from 2.5 to 6.3 for changes in scores, and from 3.8 to 22.2 for changes in score percentages. Of note, the effect of training on percentage changes in the normalized glottal gap area score was significant (P= 0.0127) after adjustment for the duration of vocal disease, body mass index and BMI, and history of gastroesophageal reflux disease.Inspiratory muscle training can improve the glottal gap after injection laryngoplasty, and may be applied in clinical practice.
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- 2022
14. Novel integrated inverter/converter circuit and control technique of motor drives with dual mode control for EV/HEV applications.
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Yen-Shin Lai, Shang-Wei Chen, Wei-Ting Lee, Yong-Kai Lin, and Jian-Feng Tsai
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- 2013
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15. The unifying catalytic mechanism of the RING-between-RING E3 ligase family
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Xiangyi S. Wang, Thomas R. Cotton, Sarah J. Trevelyan, Lachlan W. Richardson, Wei Ting Lee, John Silke, and Bernhard C. Lechtenberg
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The RING-between-RING (RBR) E3 ubiquitin ligase family in humans comprises 14 members and is defined by a two-step catalytic mechanism in which ubiquitin is first transferred from an E2 to the RBR active site and then to the substrate. To define the core features of this catalytic mechanism, we structurally and biochemically characterise the two RBRs HOIL-1 and RNF216. Crystal structures of both enzymes in their RBR/E2-Ub/Ub transthiolation complexes capturing the first catalytic step, in combination with complementary functional experiments, reveal the defining features of the RBR catalytic mechanism. RBRs catalyse ubiquitination via a conserved transthiolation complex structure that enables efficient E2-to-RBR ubiquitin transfer. Our data also highlight a conserved RBR allosteric mechanism by distinct ubiquitin linkages that suggests that RBRs employ a feed-forward mechanism accelerating their signalling pathways. We finally identify that the HOIL-1 RING2 domain contains an unusual Zn2/Cys6 bivalent zinc cluster that may represent a specific adaptation enabling HOIL-1’s atypical substrate specificity.
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- 2022
16. Probing the local-feature space of interest points.
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Wei-Ting Lee and Hwann-Tzong Chen
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- 2010
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17. Histogram-based interest point detectors.
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Wei-Ting Lee and Hwann-Tzong Chen
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- 2009
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18. ASO Visual Abstract: Distinct Failure Patterns in Hypopharyngeal Cancer Patients Receiving Surgery-Based Versus Radiation-Based Treatment
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Yu-Hsuan, Lin, Jenn-Ren, Hsiao, Yuan-Hua, Wu, Jeffrey S, Chang, Chun-Yen, Ou, Wei-Ting, Lee, Cheng-Chih, Huang, Chan-Chi, Chang, Yu-Hsuan, Lai, Sen-Tien, Tsai, Wei-Ting, Hsueh, Chia-Jui, Yen, Chen-Lin, Lin, Yu-Shan, Chen, Shih-Sheng, Jiang, Yu-Chu, Su, and Shang-Yin, Wu
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Oncology ,Surgery - Published
- 2022
19. Beneficial effect of Bletilla striata extract solution on zymosan‐induced interstitial cystitis in rat
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Ching-Chung Liang, Yi-Ching Liu, Yi-Hao Lin, Wei-Ting Lee, Tsia-Shu Lo, and Wu-Chiao Hsieh
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medicine.medical_specialty ,Urology ,media_common.quotation_subject ,medicine.medical_treatment ,030232 urology & nephrology ,Withdrawal reflex ,Urination ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Saline ,media_common ,030219 obstetrics & reproductive medicine ,business.industry ,Urethral sphincter ,Zymosan ,Interstitial cystitis ,Visceral pain ,medicine.disease ,chemistry ,Overactive bladder ,Neurology (clinical) ,medicine.symptom ,business - Abstract
Aims Interstitial cystitis (IC) is a chronic pain syndrome that is characterized by suprapubic pain upon bladder filling. Bletilla striata, a well-known traditional Chinese herb with established efficacy in wound healing and anti-inflammation, was hypothesized to improve the symptoms of IC possibly though forming a physical barrier that could isolate the bladder tissue from irritants. This study was conducted to evaluate the beneficial effects of intravesical treatment with B. striata extract solution (BSES) on visceral pain and bladder function of rats with zymosan-induced IC. Methods Thirty female rats were randomly divided into control group, zymosan-induced cystitis rats treated with normal saline (Z + NS), and zymosan-induced cystitis rats treated with BSES (Z + BSES). All rats underwent evaluation for abdominal withdrawal reflex (AWR) scores to assess visceral hypersensitivity, cystometrography, and electromyogram (EMG) of both external urethral sphincter and bladder detrusor. Data were analyzed by one way analysis of variance. Results The Z + NS group had an increased visceral hypersensitivity as compared to control group. Rats treated with BSES (Z + BSES group) had decreased AWR scores and amplitude of bladder detrusor-EMG. Besides, BSES treatment improved overactive bladder with significant effects on the extend of micturition interval and increase of storage of urine. Conclusions Intravesical instillation of BSES can significantly alleviate zymosan-induced visceral hypersensitivity and bladder overactivity associated with IC. This study suggested that intravesical instillation with BSES might be a promising treatment for IC.
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- 2021
20. Salvage Eustachian Tube Cartilage Chip Insertion After Multiple Transnasal Shim Operations in Intractable Patulous Eustachian Tube
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Wei Ting Lee and Heng Jui Hsu
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Patulous Eustachian tube ,medicine.medical_specialty ,medicine.anatomical_structure ,Otorhinolaryngology ,business.industry ,Eustachian tube ,Cartilage ,otorhinolaryngologic diseases ,Shim (computing) ,Medicine ,business ,medicine.disease ,Surgery - Abstract
This article presents 2 cases of extremely intractable patulous Eustachian tube following multiple transnasal shim insertion. These cases highlight the disadvantages of repeat transnasal shim operations, including enlargement of the Eustachian tube lumen, frequent dislocation, repeat surgery, recurrent middle ear infection, and shim misswallowing. The patients in these cases were successfully treated with Eustachian tube cartilage chip insertion through a postauricular approach. We describe the surgical technique and advantages of this promising management method.
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- 2021
21. Dynamic AODV Backup Routing in Dense Mobile Ad-Hoc Networks.
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Wen-Tsuen Chen and Wei-Ting Lee
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- 2004
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22. A heuristic method for the inventory routing problem with time windows.
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Shu-Chu Liu and Wei-Ting Lee
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- 2011
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23. Design of decentralised sliding surfaces for a class of large-scale systems with mismatched perturbations.
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Chih-Chiang Cheng, Chih-Chin Wen, and Wei-Ting Lee
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- 2009
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24. Cetyltrimethylammonium Bromide Suppresses the Migration and Invasion of Hepatic Mahlavu Cells by Modulating Fibroblast Growth Factor Signaling
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Wei-Ting Lee, Chia-Jen Lee, Tzu-Fen Su, Fu-Mei Huang, Tsai-Kun Wu, Chung-Hung Chen, and Ying-Ru Pan
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Cancer Research ,MMP2 ,Cell Survival ,medicine.medical_treatment ,Antineoplastic Agents ,Vimentin ,Fibroblast growth factor ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Viability assay ,Transcription factor ,biology ,Cetrimonium ,Chemistry ,Growth factor ,Cell Cycle ,Liver Neoplasms ,General Medicine ,Xenograft Model Antitumor Assays ,Fibroblast Growth Factors ,Disease Models, Animal ,SNAI2 ,Oncology ,030220 oncology & carcinogenesis ,SNAI1 ,Cancer research ,biology.protein ,Biomarkers ,Signal Transduction - Abstract
Background/aim Liver cancer is the fourth leading cause of cancer-related mortality globally, of which hepatocellular carcinoma (HCC) accounts for 85-90% of total primary liver cancer. A drug shortage for HCC therapy triggered us to screen the small-molecule database with a high-throughput cellular screening system. Herein, we examined whether cetyltrimethylammonium bromide (CTAB) inhibits cellular mobility and invasiveness of Mahlavu HCC cells. Materials and methods The effects of CTAB on cell viability were assessed using WST-1 assay, cell-cycle distribution using flow cytometric analysis, migration/invasion using woundhealing and transwell assays, and associated protein levels using western blotting. Results Treatment of Mahlavu cells with CTAB transformed its mesenchymal spindle-like morphology. In addition, CTAB exerted inhibitory effects on the migration and invasion of Mahlavu cells dose-dependently. CTAB also reduced the protein levels of matrix metalloproteinase-2 (MMP2), MMP9, RAC family small GTPase 1, SNAIL family transcriptional repressor 1 (SNAI1), SNAI2, TWIST family basic helix-loop-helix transcription factor 1 (TWIST1), vimentin, N-cadherin, phospho-fibroblast growth factor (FGF) receptor, phospho-phosphoinositide 3-kinase, phospho-v-Akt murine thymoma viral oncogene and phospho-signal transducer and activator of transcription 3 but increased the protein levels of tissue inhibitor of metalloproteinases-1/2 and E-cadherin. Rescue experiments proved that CTAB induced mesenchymal-epithelial transition in Mahlavu cells and this was significantly dose-dependently mitigated by basic FGF. Conclusion CTAB suppressed the migration and invasion of Mahlavu cells through inhibition of the FGF signaling pathway. CTAB seems to be a potential agent for preventing metastasis of hepatic cancer.
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- 2020
25. Cetyltrimethylammonium Bromide Disrupts the Mesenchymal Characteristics of HA22T/VGH Cells Via Inactivation of c-Met/PI3K/Akt/mTOR Pathway
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Tzu-Fen Su, Wei-Ting Lee, Chia-Jen Lee, Ying-Ru Pan, Yi-Ping Chen, Chia-Herng Yue, Fu-Mei Huang, and Chung-Hung Chen
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Cancer Research ,Matrigel ,C-Met ,biology ,Vimentin ,General Medicine ,Cell cycle ,chemistry.chemical_compound ,Oncology ,chemistry ,Ribosomal protein s6 ,biology.protein ,Cancer research ,medicine ,Hepatocyte growth factor ,Protein kinase B ,PI3K/AKT/mTOR pathway ,medicine.drug - Abstract
Background/aim Hepatocellular carcinoma (HCC) arises from hepatocytes, and is the most frequently occurring malignancy of primary liver cancer. In this study, we investigated the anti-metastatic effects of the quaternary ammonium compound, cetyltrimethylammonium bromide (CTAB), on HA22T/VGH HCC cells. Materials and methods According to our preliminary data, the effect of CTAB on cell cycle distribution, migration, invasion and the associated protein levels was examined using flow cytometry, wound-healing migration, Matrigel transwell invasion assay and western blotting under sub-lethal concentrations. Results CTAB treatment of HA22T/VGH cells casued dose-dependent mesenchymal-epithelial transition (MET)-like changes and impaired migration and invasion capabilities. In addition, CTAB reduced the levels of metastasis-related proteins including c-Met, phosphoinositide 3-kinase (PI3K), Akt, mammalian target of rapamycin (mTOR), ribosomal protein S6 kinase (p70S6K), Twist, N-cadherin, and Vimentin. Moreover, pretreatment with hepatocyte growth factor (HGF) rescued CTAB-mediated effects. Conclusion CTAB exhibited potent anti-EMT and anti-metastatic activities through the inhibition of migration and invasion of HA22T/VGH cells. CTAB interrupted the mesenchymal characteristics of HA22T/VGH cells, which were significantly alleviated by HGF in a dose-dependent manner. CTAB has the potential to evolve as a therapeutic agent for HCC.
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- 2020
26. Investigating the health disparities in the association between lifestyle behaviors and the risk of head and neck cancer
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Chia Jui Yen, Wei Ting Lee, Han Chien Yang, Yuan Hua Wu, Shang Yin Wu, Chun Yen Ou, Yu Shan Chen, Cheng Chih Huang, Jeffrey S. Chang, Yu Hsuan Lai, Chan Chi Chang, Jenn Ren Hsiao, Ya Ling Weng, Jehn Shyun Huang, Sen Tien Tsai, Wei Ting Hsueh, Jang Yang Chang, Chen Lin Lin, and Ken Chung Chen
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Male ,0301 basic medicine ,Cancer Research ,Psychological intervention ,0302 clinical medicine ,Risk Factors ,Prevalence ,risk ,biology ,alcohol ,Aldehyde Dehydrogenase, Mitochondrial ,Smoking ,Oxides ,General Medicine ,Middle Aged ,Betel ,Health equity ,Oncology ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Educational Status ,Original Article ,Female ,Alcohol Drinking ,Taiwan ,Polymorphism, Single Nucleotide ,socioeconomic status ,03 medical and health sciences ,Environmental health ,case‐control ,Genetic predisposition ,medicine ,Humans ,Genetic Predisposition to Disease ,Life Style ,Socioeconomic status ,Plant Extracts ,Squamous Cell Carcinoma of Head and Neck ,business.industry ,Head and neck cancer ,Epidemiology and Prevention ,Original Articles ,Health Status Disparities ,Odds ratio ,Calcium Compounds ,biology.organism_classification ,medicine.disease ,Confidence interval ,030104 developmental biology ,Social Class ,Case-Control Studies ,Universal Health Care ,head and neck cancer ,business ,Piper - Abstract
Many studies have reported a positive association between lower socioeconomic status (SES) and higher head and neck cancer (HNC) risk. Fewer studies have examined the impact of SES on the association between alcohol or cigarette use and HNC risk. The current case‐control study (1104 HNC cases and 1363 controls) investigated the influence of education, a SES indicator, on the association between HNC and the use of alcohol, cigarettes, or betel quids in Taiwan, a country with universal health care. Our results showed a larger increase in HNC risk associated with alcohol among those with lower educational level (odds ratio [OR] = 2.07; 95% confidence interval [CI], 1.53‐2.80) than those with higher educational level (OR = 1.38; 95% CI, 1.04‐1.85) (heterogeneity‐P = .03). Educational level had an influence on the association between alcohol use and HNC risk among those with genetic susceptibility (ALDH2‐deficient) to the carcinogenic effect of alcohol. The association between cigarette or betel quid use and HNC risk was similar between the high and low educational groups. National policies and social interventions have led to the decline in the prevalence of cigarette and betel quid users in Taiwan. In contrast, due to the lack of adequate alcohol control policies, alcohol consumption in Taiwan has continued to rise. A higher impact of alcohol on HNC risk among lower SES individuals even with universal health care could be the result of insufficient alcohol control policies in Taiwan.
- Published
- 2020
27. Evaluation of Objective and Subjective Swallowing Outcomes in Patients with Dysphagia Treated for Head and Neck Cancer
- Author
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Hsin-Hao Liou, Shu-Wei Tsai, Miyuki Hsing-Chun Hsieh, Yi-Jen Chen, Jenn-Ren Hsiao, Cheng-Chih Huang, Chun-Yen Ou, Chan-Chi Chang, Wei-Ting Lee, Sen-Tien Tsai, and David Shang-Yu Hung
- Subjects
head and neck cancer ,dysphagia ,swallowing ,subjective assessment ,objective assessment ,outcomes ,digestive, oral, and skin physiology ,otorhinolaryngologic diseases ,Medicine ,General Medicine - Abstract
We evaluated objective and subjective swallowing function outcomes in patients with dysphagia treated for head and neck cancer (HNC) and identified risk factors for poor swallowing outcomes. Patients undergoing videofluoroscopic swallowing studies (VFSS) between January 2016 and March 2021 were divided into four groups according to primary tumor sites; post-treatment dysphagia was assessed. The penetration–aspiration scale (PAS) and bolus residue scale (BRS) were used to objectively assess swallowing function through VFSS. The Functional Oral Intake Scale (FOIS) was used for subjective analyses of swallowing statuses. To account for potential confounding, important covariates were adjusted for in logistic regression models. Oropharyngeal tumors were significantly more likely to have poor PAS and BRS scores than oral cavity tumors, and the patients with nasopharyngeal tumors were significantly less likely to have poor FOIS scores. Old age, having multiple HNCs, and a history of radiotherapy were associated with an increased odds of poor PAS scores (for all types of swallows), poor BRS scores (for semiliquid and solid swallows), and poor FOIS scores, respectively. This indicates using only subjective assessments may not allow for accurate evaluations of swallowing function in patients treated for HNC. Using both objective and subjective assessments may allow for comprehensive evaluations.
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- 2022
- Full Text
- View/download PDF
28. The exosome-mediated autocrine and paracrine actions of plasma gelsolin in ovarian cancer chemoresistance
- Author
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Euridice Carmona, Pei Wen Wang, Meshach Asare-Werehene, F. Jeffrey Dilworth, Dylan Burger, Chen Tzu Chiu, Anne Marie Mes-Masson, Tien Le, Benjamin K. Tsang, Dar-Bin Shieh, Kiran Nakka, Wei Ting Lee, Arkadiy Reunov, and Mohammad R. Abedini
- Subjects
0301 basic medicine ,Cancer Research ,endocrine system diseases ,Apoptosis ,Cell fate determination ,Biology ,Exosomes ,Exosome ,Article ,03 medical and health sciences ,Paracrine signalling ,0302 clinical medicine ,Ovarian cancer ,Paracrine Communication ,Genetics ,medicine ,Humans ,Autocrine signalling ,Molecular Biology ,Gelsolin ,Ovarian Neoplasms ,Regulation of gene expression ,Hypoxia-Inducible Factor 1, alpha Subunit ,medicine.disease ,female genital diseases and pregnancy complications ,Microvesicles ,Up-Regulation ,3. Good health ,Gene Expression Regulation, Neoplastic ,Autocrine Communication ,030104 developmental biology ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer research ,Female ,Extracellular signalling molecules ,Proto-Oncogene Proteins c-akt ,Integrin alpha5beta1 ,Signal Transduction - Abstract
Ovarian cancer (OVCA) is the most lethal gynecological cancer, due predominantly to late presentation, high recurrence rate and common chemoresistance development. The expression of the actin-associated protein cytosolic gelsolin (GSN) regulates the gynecological cancer cell fate resulting in dysregulation in chemosensitivity. In this study, we report that elevated expression of plasma gelsolin (pGSN), a secreted isoform of GSN and expressed from the same GSN gene, correlates with poorer overall survival and relapse-free survival in patients with OVCA. In addition, it is highly expressed and secreted in chemoresistant OVCA cells than its chemosensitive counterparts. pGSN, secreted and transported via exosomes (Ex-pGSN), upregulates HIF1α–mediated pGSN expression in chemoresistant OVCA cells in an autocrine manner as well as confers cisplatin resistance in otherwise chemosensitive OVCA cells. These findings support our hypothesis that exosomal pGSN promotes OVCA cell survival through both autocrine and paracrine mechanisms that transform chemosensitive cells to resistant counterparts. Specifically, pGSN transported via exosomes is a determinant of chemoresistance in OVCA.
- Published
- 2019
29. Relationship Between Pharyngeal Residues Assessed by Bolus Residue Scale or Normalized Residue Ratio SCALE and Risk of Aspiration in Head and Neck Cancer Who Underwent Videofluoroscopy
- Author
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Hsin-Hao Liou, Miyuki Hsing-Chun Hsieh, Sheng-Han Tsai, David Shang-Yu Hung, Yi-Jen Chen, Jenn-Ren Hsiao, Cheng-Chih Huang, Chun-Yen Ou, Chan-Chi Chang, Wei-Ting Lee, Sen-Tien Tsai, and Shu-Wei Tsai
- Subjects
Speech and Hearing ,Otorhinolaryngology ,Gastroenterology - Abstract
Dysphagia affects 60-75% of patients treated for head and neck cancer (HNC). We aimed to evaluate the association between residue severity and airway invasion severity using a videofluoroscopic swallowing study and identify risk factors for poor penetration-aspiration outcomes in patients with dysphagia treated for HNC. Penetration-Aspiration Scale (PAS) was used to assess airway invasion severity, while residue severity was assessed using both the Bolus Residue Scale (BRS) for residue location and the Normalized Residue Ratio Scale (NRRS) for residue amount. Relevant covariates were adjusted in the logistic regression models to account for potential confounding. Significantly higher abnormal PAS was reported for increased piriform sinus NRRS (NRRSp) [odds ratio (OR), 4.81; p = 0.042] with liquid swallowing and increased BRS value (OR, 1.52; p = 0.014) for semi-liquid swallowing in multivariate analysis. Tumor location, older age, and poorer Functional Oral Intake Scale (FOIS) were significant factors for abnormal PAS in both texture swallowings. After adjusting for confounding factors (sex, age, and FOIS score), NRRS model in liquid swallowing (area under the curve [AUC], 0.83; standard error = 0.04, 95% confidence interval [CI]: 0.75, 0.91) and BRS in semi-liquid swallowing (AUC, 0.83; SE = 0.04; 95% CI: 0.76, 0.91) predicted abnormal PAS. The results indicate that while assessing residue and swallowing aspiration in patients with HNC, it is important to consider age, tumor location, and functional swallowing status. The good predictability of abnormal PAS with BRS and NRRS indicated that residue location and amount were both related to the aspiration event in patients with HNC.
- Published
- 2021
30. Three Cases of Parotid Hemangiomas in Adults
- Author
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Yi-Ting Huang, Chun-Yen Ou, Wei-Ting Lee, and Heng-Jui Hsu
- Subjects
stomatognathic diseases ,stomatognathic system ,Otorhinolaryngology ,sense organs ,eye diseases - Abstract
Hemangiomas account for only 0.4% to 0.6% of all parotid tumors, making them extremely rare in adults. Unlike pediatric parotid hemangiomas, those in adults typically present as asymptomatic swellings of the parotid, have no skin discoloration, and usually do not regress spontaneously. Therefore, an accurate diagnosis of parotid hemangiomas in adults before surgical excision is generally challenging. Herein, we present 3 cases of adult parotid hemangiomas. The patients all received parotidectomies with tumor resection. Histopathological analysis of the resected specimens revealed numerous dilated, thin- or thick-walled (small, large, or variably sized) vessels lined with flattened endothelial cells. A diagnosis of cavernous hemangioma of the parotid gland was established only after the histopathological analysis. Parotid cavernous hemangiomas in adults are rare and often misdiagnosed before surgical resection. Clinical presentation and imaging features on ultrasonography, computed tomography, magnetic resonance imaging, and angiography may support an accurate preoperative diagnosis. Surgical resection proved a suitable treatment approach for our 3 cases.
- Published
- 2021
31. Prediction of the BCI results For CAN bus ECU using incident wave excitation method.
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Wei Ting Lee, Yung Chi Chung, Chu Yu Chen, Jing-Jou Tang, and Chung Shun Yang
- Published
- 2014
- Full Text
- View/download PDF
32. Revealing Tripartite Quantum Discord with Tripartite Information Diagram.
- Author
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Wei-Ting Lee and Che-Ming Li
- Published
- 2017
- Full Text
- View/download PDF
33. Endoscopy-Assisted Transmeatal Cochlear Implantation in Multiple Ear Deformities
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Yi-Lu Li, Jiunn Liang Wu, and Wei-Ting Lee
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medicine.medical_specialty ,medicine.medical_treatment ,Cochlear implant ,otorhinolaryngologic diseases ,medicine ,Humans ,Inner ear ,Child ,Sigmoid sinus ,Round window ,medicine.diagnostic_test ,business.industry ,Microtia ,Endoscopy ,General Medicine ,medicine.disease ,Facial nerve ,Cochlear Implantation ,Surgery ,Cochlea ,medicine.anatomical_structure ,Cochlear Implants ,RF1-547 ,Otorhinolaryngology ,Round Window, Ear ,Middle ear ,sense organs ,business - Abstract
To study congenitally deaf children with inner ear malformations that usually have comorbid anomalous facial nerves and middle ear deformities. To determine the feasibility of endoscopy-assisted transmeatal cochlear implantation with the purpose of reducing the risks of iatrogenic facial nerve injury. This report presents a unique technique in a pediatric case with multiple ear anomalies: microtia, cochlear hypoplasia with an aberrant facial nerve, a sigmoid sinus deformity leading to a narrow mastoid cavity, and a flat promontory wall without round window. A cochlear implant electrode array was successfully inserted endoscopically using the transmeatal approach in the present case. It caused no postoperative surgical complications, and the patient was then able to hear binaurally and functionally. For patients with comorbid multiple ear deformities, using endoscopy for cochlear implantation transmeatally is an alternative method providing a better visualization of the middle ear anatomy, an avoidance of injury of facial nerve, and an assurance of precise insertion of the electrode when the posterior tympanotomy approach is not applicable.
- Published
- 2021
34. Disputed rpoB Mutations in Mycobacterium tuberculosis and Tuberculosis Treatment Outcomes
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Wei-Ting Lee, Ruwen Jou, Hsing-Yuan Tsai, and Wan-Hsuan Lin
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Rifabutin ,Tuberculosis ,Treatment outcome ,Antitubercular Agents ,Microbial Sensitivity Tests ,medicine.disease_cause ,Mycobacterium tuberculosis ,03 medical and health sciences ,0302 clinical medicine ,Bacterial Proteins ,Tuberculosis, Multidrug-Resistant ,Genotype ,medicine ,Humans ,Pharmacology (medical) ,030212 general & internal medicine ,Pharmacology ,0303 health sciences ,Mutation ,biology ,030306 microbiology ,business.industry ,DNA-Directed RNA Polymerases ,Odds ratio ,biology.organism_classification ,rpoB ,medicine.disease ,Virology ,Treatment Outcome ,Infectious Diseases ,Susceptibility ,business ,medicine.drug - Abstract
Discordant results between genotypic drug susceptibility testing (gDST) and phenotypic DST (pDST) for Mycobacterium tuberculosis isolates with disputed (discordance between gDST and pDST results) mutations affect rifampin (RIF)-resistant (RR) and multidrug-resistant (MDR) tuberculosis (TB) treatments due to a lack of practical clinical guidelines. To investigate the role of disputed rpoB mutations in M. tuberculosis and TB treatment outcomes, initial isolates of 837 clinical RR- or MDR-TB cases confirmed during 2014 to 2018 were retested using agar-based RIF pDST and rpoB gene sequencing. MICs were determined for isolates with disputed rpoB mutations. Disputed rpoB mutations were identified in 77 (9.2%) M. tuberculosis isolates, including 50 (64.9%) and 14 (18.2%) phenotypically RIF- and rifabutin (RFB)-resistant isolates, respectively. The predominant single mutations were those encoding L533P (a change of L to P at position 533) (44.2%) and L511P (20.8%). Most of the isolates harboring mutations encoding L511P (87.5%), H526N (100%), D516Y (70.0%), and L533P (63.6%) had MICs of ≤1 mg/liter, whereas isolates harboring the mutation encoding H526L (75%) had a MIC of >1 mg/liter. Of the 63 cases with treatment outcomes available, 11 (17.5%) cases died, 1 (1.6%) case transferred out, and 51 (81%) cases had favorable outcomes, including 8 and 20 cases treated with standard-dose RIF- and RFB-containing regimens, respectively. Excluding cases that transferred out or received no or 1-day treatment, we observed statistically significant differences between the outcomes using active and inactive fluoroquinolones (FQs) (P = 0.008, odds ratio = 0.05 [95% confidence interval, 0.01 to 0.38]) in 57 cases (where active means a case susceptible to the drug and inactive means a case resistant to the drug or drug not used). We concluded that disputed rpoB mutations are not rare. Depending on the resources available, sequencing and/or MIC testing is recommended for better management of RR- and MDR-TB cases.
- Published
- 2021
35. Redefining MDR-TB: Comparison of Mycobacterium tuberculosis clinical isolates from Russia and Taiwan
- Author
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Elena Nosova, Jui-Yun Weng, Wan-Hsuan Lin, Mei-Hua Wu, Wei-Ting Lee, Alexandra I. Isakova, Ruwen Jou, Danila Vadimovich Zimenkov, Liaisan R. Arslanbaeva, Olga V. Antonova, Hsing-Yuan Tasi, and Elena V. Kulagina
- Subjects
0301 basic medicine ,Microbiology (medical) ,Tuberculosis ,Extensively Drug-Resistant Tuberculosis ,030106 microbiology ,Antitubercular Agents ,Taiwan ,Microbial Sensitivity Tests ,Drug resistance ,Microbiology ,Russia ,Mycobacterium tuberculosis ,03 medical and health sciences ,Bacterial Proteins ,Drug Resistance, Multiple, Bacterial ,Tuberculosis, Multidrug-Resistant ,Genetics ,medicine ,Humans ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Retrospective Studies ,biology ,Isoniazid ,Retrospective cohort study ,Kanamycin ,biology.organism_classification ,medicine.disease ,Virology ,030104 developmental biology ,Infectious Diseases ,Streptomycin ,Mutation ,Genes, MDR ,Genome, Bacterial ,Rifampicin ,medicine.drug - Abstract
Multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis are global challenges due to the limited number of effective drugs for treatment. Treatment with less than 4–5 effective drugs might lead to the further emergence of drug resistance and poor clinical outcomes. For better prediction of treatment outcomes, we compared drug-resistance profiles of consecutive clinical MDR Mycobacterium tuberculosis isolates from high- and low-burden settings. This was a retrospective cohort study. We analysed 225 and 229 MDR isolates from Moscow (Russia) and Taiwan, respectively, obtained between 2014 and 2015. Drug susceptibility testing was performed by the Bactec MGIT 960 automated system and the agar proportion method. Detection of resistance-associated mutations in the M. tuberculosis genome was carried out by an array and/or sequencing of selected loci. The principal differences between resistance profiles of MDR isolates in the two countries were the percentages of pre-XDR (40.9% vs. 14.8%) and XDR (34.7% vs. 1.7%) isolates, both of which were significantly higher in Moscow isolates. Forty-eight (33%) of 147 MDR and pre-XDR Russian isolates fall into a group with less than four effective drugs, which accounts for 40% (N = 120) of these isolates. The other 60% in this group were XDR strains (N = 72). Consequently, the average number of effective anti-tuberculosis drugs for MDR-TB treatment was lower for Russian isolates (3 vs. 7). Furthermore, a notable percentage (9%) of isolates resistant to kanamycin harboured mutations in the whiB7 locus, which was not detected by molecular tests targeting common mutations in the rrs and eis loci. We found that 98.2% and 45.9% of MDR isolates from Moscow and Taiwan, respectively, were resistant to streptomycin. Molecular tests for detecting resistance to drugs other than rifampicin, isoniazid, fluoroquinolones, and second-line injectable drugs are needed for individualized therapy. The conventional MDR treatment schemes most probably fail in these cases due to the limited number of effective drugs.
- Published
- 2019
36. Treatment outcomes for one-stage concurrent surgical resection and reconstruction of synchronous esophageal and head and neck squamous cell carcinoma
- Author
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Chun Yen Ou, Yu Hsuan Lin, Wei Ting Lee, Yao Chou Lee, Tzu Yen Chang, and Yi Ting Yen
- Subjects
Adult ,Male ,Larynx ,medicine.medical_specialty ,Esophageal Neoplasms ,Taiwan ,Anastomotic Leak ,Anastomosis ,Neoplasms, Multiple Primary ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,030223 otorhinolaryngology ,Aged ,Neoplasm Staging ,Retrospective Studies ,business.industry ,Dissection ,Anastomosis, Surgical ,Head and neck cancer ,Retrospective cohort study ,General Medicine ,Middle Aged ,Plastic Surgery Procedures ,medicine.disease ,Head and neck squamous-cell carcinoma ,Surgery ,Squamous carcinoma ,Outcome and Process Assessment, Health Care ,Treatment Outcome ,medicine.anatomical_structure ,Otorhinolaryngology ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Female ,Esophageal Squamous Cell Carcinoma ,business ,Complication ,Follow-Up Studies - Abstract
It is not uncommon to see the synchronous presentation of esophageal squamous carcinoma (ESCC) and head and neck cancer (HNC), and most patients were treated with staged interventions. This study retrospectively reported the outcomes of patients with synchronous ESCC and HNC treated with one-stage concurrent surgical resection and reconstruction. We identified 17 consecutive patients with synchronous ESCC and HNC undergoing primary concurrent surgical resections between 2011 and 2017 at our hospital. All patients had received esophageal screenings prior to treatment. The HNC patients in this study had the following subsite involvements: oral cavity (n = 5), oropharynx (n = 4), larynx (n = 1), hypopharynx (n = 9), and thyroid gland (n = 1). Eighty percent of the HNC subsites (16/20) were treated in advanced stages, while most ESCCs were treated at early stages. The mean follow-up time was 3.2 ± 1.6 years. Surgery-associated morbidity and mortality were 94.1% and 0%, respectively, and the most common complication was anastomotic leakage. The two-year overall survival, 2-year loco-regional recurrence-free survival, and 2-year distant metastasis-free survival were 86.7%, 85.6%, and 78.7%, respectively. No significant difference was found between overall survival and HNC subsite or anastomotic leakage. Four patients (23.5%) developed secondary primary malignancies (SPMs) within a mean follow-up period of 2.9 years (standard deviation 1.6 years). Although one-stage concurrent surgical resection and reconstruction of synchronous ESCC and HNC were highly invasive and complicated, survival was promising. Isolated distant metastasis remained the most common failure pattern. Vigilant follow-up strategy is mandatory to detect secondary primary malignancies (SPMs), especially within the first 3 years following initial treatment.
- Published
- 2019
37. Oral hygiene and the overall survival of head and neck cancer patients
- Author
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Wei Ting Lee, Chia Jui Yen, Sen Tien Tsai, Wei Ting Hsueh, Chen Lin Lin, Cheng Chih Huang, Chun Yen Ou, Han Chien Yang, Tung Yiu Wong, Jeffrey S. Chang, Yu Hsuan Lai, Jang Yang Chang, Jehn Shyun Huang, Chan Chi Chang, Ken Chung Chen, Jenn Ren Hsiao, Yu Shan Chen, Yuan Hua Wu, Shang Yin Wu, and Ya Ling Weng
- Subjects
Adult ,Male ,0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,Genotype ,Health Behavior ,Taiwan ,epidemiology and prevention ,Single-nucleotide polymorphism ,Polymorphism, Single Nucleotide ,Oral hygiene ,survival ,lcsh:RC254-282 ,Tooth brushing ,03 medical and health sciences ,Dental floss ,0302 clinical medicine ,Internal medicine ,medicine ,gene–environmental interaction ,Humans ,Radiology, Nuclear Medicine and imaging ,Registries ,Risk factor ,Life Style ,Original Research ,business.industry ,Hazard ratio ,Head and neck cancer ,Middle Aged ,Oral Hygiene ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Survival Analysis ,Confidence interval ,Neoplasm Proteins ,Toll-Like Receptor 4 ,stomatognathic diseases ,030104 developmental biology ,Oncology ,Head and Neck Neoplasms ,Case-Control Studies ,030220 oncology & carcinogenesis ,Female ,Gene-Environment Interaction ,head and neck cancer ,business ,Cancer Prevention - Abstract
Poor oral hygiene is an established risk factor of head and neck cancer (HNC); however, its role in the survival of HNC patients is unclear. This study evaluated the association between oral hygiene habits, including regular dental visits, frequency of tooth brushing, and use of dental floss, and the overall survival (OS) of HNC patients using interview data collected from 740 HNC patients. In addition, the interactions between oral hygiene and the polymorphisms of TLR2 and TLR4 on the OS of HNC patients were assessed. The analysis indicated that poor oral hygiene was significantly associated with poorer OS of HNC patients (hazard ratio (HR) = 1.38, 95% confidence interval (CI): 1.03‐1.86). This association was modified by a single nucleotide polymorphism, rs11536889, of TLR4. A significant association between poor oral hygiene and worse survival of HNC was observed among those with the CG or CC genotype (HR = 2.32, 95% CI: 1.41‐3.82) but not among those with the GG genotype (HR = 0.95, 95% CI: 0.65‐1.40). Our results suggested that poor oral hygiene is not only a risk factor but may also be a prognostic factor of HNC., Poor oral hygiene was associated with a worse survival of head and neck cancer. TLR4 affected the survival of head and neck cancer due to poor oral hygiene. Poor oral hygiene is both a risk and a prognostic factor of head and neck cancer
- Published
- 2019
38. Investigating the association between serum human papillomavirus type 16 E7 antibodies and risk of head and neck cancer
- Author
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Han Chien Yang, Cheng Chih Huang, Shang Yin Wu, Chia Jui Yen, Jenn Ren Hsiao, Ken Chung Chen, Jeffrey S. Chang, Yu Hsuan Lai, Yuan Hua Wu, Yu Chu Su, Mei Ling Tsai, Jehn Shyun Huang, Chan Chi Chang, Ya Ling Weng, Chun Yen Ou, Wei Ting Lee, Sen Tien Tsai, Wei Ting Hsueh, Yu Shan Chen, Jang Yang Chang, and Chen Lin Lin
- Subjects
0301 basic medicine ,Male ,Cancer Research ,Papillomavirus E7 Proteins ,Antibodies, Viral ,0302 clinical medicine ,cancer risk factors ,Risk Factors ,Epidemiology ,RC254-282 ,Original Research ,Human papillomavirus 16 ,biology ,Smoking ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,virus diseases ,Middle Aged ,Betel ,Oropharyngeal Neoplasms ,Oncology ,Head and Neck Neoplasms ,030220 oncology & carcinogenesis ,Female ,epidemiology ,Antibody ,Cancer Prevention ,medicine.medical_specialty ,Alcohol Drinking ,Taiwan ,03 medical and health sciences ,Sex Factors ,Internal medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Risk factor ,Areca ,Cancer prevention ,business.industry ,Public health ,Head and neck cancer ,Cancer ,medicine.disease ,biology.organism_classification ,stomatognathic diseases ,030104 developmental biology ,nervous system ,Case-Control Studies ,biology.protein ,head and neck cancer ,business - Abstract
Human papillomavirus (HPV) is recognized as a major cause of oropharyngeal cancer (OPC) in Western countries. Less is known regarding its contribution to the OPC occurring in Asia. The current study aimed to investigate the association between antibody responses to HPV16 E7 and head and neck cancer (HNC) risk in a hospital‐based case–control study conducted in Taiwan with 693 HNC cases and 1,035 controls. A positive association was observed between seropositivity to HPV16 E7 and OPC risk, whereas no significant association was found in the non‐OPC cases. The increased OPC risk associated with seropositivity to HPV16 E7 was more significant among nonbetel quid or noncigarette users. Seropositivity to HPV16 E7 showed moderate agreement with P16 expression in OPC. OPC patients that were seropositive to HPV16 E7 or p16 positive were more highly educated and less likely to use alcohol, betel quids, and cigarettes compared to HPV16 E7 seronegative or p16 negative OPC patients. Furthermore, patients with p16 positive OPC were more likely to be women compared to patients with p16 negative OPC, likely owing to the low prevalence of alcohol, betel quid, and cigarette users among women. Overall, this study suggested that similar to Western countries, HPV may also be an important risk factor of OPC in Taiwan. With the declining consumption of betel quids and cigarettes in Taiwan, a higher percentage of OPC cases in Taiwan will be attributed to HPV in the future. Public health measures, including HPV vaccination, need to be implemented to prevent the occurrence of HPV‐positive OPC., The current study found a a positive association between seropositivity to HPV16 E7 and oropharyngeal cancer risk. This study suggested that similar to Western countries, HPV may also be an important risk factor of OPC in Taiwan.
- Published
- 2021
39. Author's reply: Beneficial effect of Bletilla striata extract solution on zymosan‐induced interstitial cystitis in rat
- Author
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Wu-Chiao Hsieh, Yi-Ching Liu, Wei-Ting Lee, Tsia-Shu Lo, Yi-Hao Lin, and Ching-Chung Liang
- Subjects
biology ,Plant Extracts ,business.industry ,Urology ,Zymosan ,Cystitis, Interstitial ,Interstitial cystitis ,Pharmacology ,biology.organism_classification ,medicine.disease ,Rats ,chemistry.chemical_compound ,chemistry ,Bletilla striata ,medicine ,Animals ,Neurology (clinical) ,Orchidaceae ,business - Published
- 2021
40. Healing the gap: the evolution of esophageal substitute and quality of life in patients undergoing total pharyngolaryngoesophagectomy with reconstruction
- Author
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Yau Lin Tseng, Wen-Ruei Tang, Ying-Yuan Chen, Wei-Ting Lee, Wei-Li Huang, Chao-Chun Chang, Jenn Ren Hsiao, Yao-Chou Lee, Jung-Der Wang, Tzu-Yen Chang, and Yi-Ting Yen
- Subjects
medicine.medical_specialty ,Quality of life (healthcare) ,business.industry ,Negative-pressure wound therapy ,medicine.medical_treatment ,medicine ,In patient ,business ,Surgery - Published
- 2021
41. Deployment of a Forward Medical Post to Provide Medical Support in a Purpose-built Dormitory during the COVID-19 Pandemic
- Author
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Wei Ting Lee, Guan Lin Lee, and Wai Leong Kok
- Subjects
2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,MEDLINE ,Patient Isolation ,COVID-19 Testing ,Residence Characteristics ,Pandemic ,Medicine ,Humans ,Transients and Migrants ,Infection Control ,Singapore ,Primary Health Care ,business.industry ,SARS-CoV-2 ,COVID-19 ,General Medicine ,medicine.disease ,Medical support ,Early Diagnosis ,Software deployment ,Communicable Disease Control ,Housing ,Medical emergency ,Prevention control ,business - Published
- 2020
42. Lingual Osteoma-A Case Report and Literature Review
- Author
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Heng Jui Hsu, Wei Ting Lee, and Hsueh An Sun
- Subjects
medicine.medical_specialty ,Tongue Base ,Asymptomatic ,Tongue Diseases ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Tongue ,medicine ,Humans ,030223 otorhinolaryngology ,Osteoma ,business.industry ,medicine.disease ,Surgery ,Tongue Neoplasms ,body regions ,medicine.anatomical_structure ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,Osseous choristoma ,Foreign body ,medicine.symptom ,business - Abstract
Osteomas are rare benign bony tumors located in the tongue area. In most cases, patients presenting with a lingual osteoma are asymptomatic but may complain of the sensation of having a foreign body. Fewer than 100 cases of lingual osteoma have been reported worldwide. Here, we present 2 cases of tongue base osteoma that were treated with excision under a laryngoscope.
- Published
- 2020
43. Digitally Fabricated Dentures for Full Mouth Rehabilitation with Zirconia, Polyetheretherketone and Selective Laser Melted Ti-6Al-4V Material
- Author
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Wei-Ting Lee and Yung-Chung Chen
- Subjects
Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health - Abstract
CAD/CAM technologies have been embedded into the fabrication of removable partial denture (RPD). Various materials such as zirconia and polyetheretherketone (PEEK) are developed for subtractive manufacturing. As for additive manufacturing, dental professionals have begun to use selective laser melting (SLM) techniques for fabricating metallic RPD frameworks. This report demonstrates a case rehabilitated with a maxillary telescopic crown-retained combining PEEK and zirconia material denture and a mandibular Kennedy Class I RPD fabricated with SLM techniques. First, a conventional impression was performed and the master cast was mounted with a centric relation record. Digital models were obtained using tabletop scanners and then the telescopic primary zirconia crowns were designed and milled. After transferring the intraoral distribution of primary crowns using pick-up impression, secondary PEEK crowns and framework were designed, milled, and veneered with composite resin. Mandibular framework was designed and constructed using SLM technique with Ti-6Al-4V. Definitive prostheses for both jaws were finished and delivered. Delivered prostheses functioned well for a one-year period. The was patient satisfied with the improvements in chewing function and esthetics. Both substrative and additive manufacturing techniques are suitable for framework fabrication. Further investigation is needed for improving the mechanical performance and long-term prognosis of digitally made prostheses.
- Published
- 2022
44. A Glutamine Insertion at Codon 432 of RpoB Confers Rifampicin Resistance in Mycobacterium tuberculosis
- Author
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Li-Yin Lai, Li-Yu Hsu, Shang-Hui Weng, Shuo-En Chung, Hui-En Ke, Tzu-Lung Lin, Pei-Fang Hsieh, Wei-Ting Lee, Hsing-Yuan Tsai, Wan-Hsuan Lin, Ruwen Jou, and Jin-Town Wang
- Subjects
Microbiology (medical) ,Tuberculosis ,medicine.drug_class ,Antibiotics ,lcsh:QR1-502 ,Drug resistance ,Biology ,rifampicin ,Microbiology ,lcsh:Microbiology ,Mycobacterium tuberculosis ,03 medical and health sciences ,Plasmid ,medicine ,polycyclic compounds ,heterocyclic compounds ,codon 432 of RpoB ,030304 developmental biology ,Original Research ,0303 health sciences ,drug resistance ,030306 microbiology ,biochemical phenomena, metabolism, and nutrition ,rpoB ,medicine.disease ,biology.organism_classification ,bacterial infections and mycoses ,Complementation ,bacteria ,Rifampicin ,medicine.drug - Abstract
Tuberculosis (TB) is an infectious respiratory disease caused by Mycobacterium tuberculosis and one of the top 10 causes of death worldwide. Treating TB is challenging; successful treatment requires a long course of multiple antibiotics. Rifampicin (RIF) is a first-line drug for treating TB, and the development of RIF-resistant M. tuberculosis makes treatment even more difficult. To determine the mechanism of RIF resistance in these strains, we searched for novel mutations by sequencing. Four isolates, CDC-1, CDC-2, CDC-3, and CDC-4, had high-level RIF resistance and unique mutations encoding RpoB G158R, RpoB V168A, RpoB S188P, and RpoB Q432insQ, respectively. To evaluate their correlation with RIF resistance, plasmids carrying rpoB genes encoding these mutant proteins were transfected into the H37Rv reference strain. The plasmid complementation of RpoB indicated that G158R, V168A, and S188P did not affect the MIC of RIF. However, the MIC of RIF was increased in H37Rv carrying RpoB Q432insQ. To confirm the correlation between RIF resistance and Q432insQ, we cloned an rpoB fragment carrying the insertion (encoding RpoB Q432insQ) into H37Rv by homologous recombination using a suicide vector. All replacement mutants expressing RpoB Q432insQ were resistant to RIF (MIC > 1 mg/L). These results indicate that RpoB Q432insQ causes RIF resistance in M. tuberculosis.
- Published
- 2020
45. π-Impedance Matching Circuit for Wireless Power Transfer Systems under Dynamic Load and Transfer Distance
- Author
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Ming-Lung Kung, Wei-Ting Lee, and Ken-Huang Lin
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Computer science ,business.industry ,020208 electrical & electronic engineering ,Impedance matching ,020206 networking & telecommunications ,02 engineering and technology ,Dynamic load testing ,Control theory ,Robustness (computer science) ,Transfer (computing) ,0202 electrical engineering, electronic engineering, information engineering ,Wireless ,Wireless power transfer ,Ohm ,business ,Coupling coefficient of resonators - Abstract
In recent years, wireless charging has become a trend because it is convenient and safe. However, its efficiency can deteriorate when the distance between coils or the load varies. In this paper, an automatic $\pi$ - impedance matching method is proposed to obtain higher efficiency under dynamic-coupling or varied-load condition. The simulation results show that the efficiency can remain higher than 85% in varying transfer distances when coupling coefficient is over 0.3. For varied-load, the efficiency is higher than 80% when the load is over 20 ohm and reach around 88% as the load increases at the 6.78-MHz operating frequency.
- Published
- 2020
46. Two Novel katG Mutations Conferring Isoniazid Resistance in Mycobacterium tuberculosis
- Author
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Li-Yu Hsu, Li-Yin Lai, Pei-Fang Hsieh, Tzu-Lung Lin, Wan-Hsuan Lin, Hsing-Yuan Tasi, Wei-Ting Lee, Ruwen Jou, and Jin-Town Wang
- Subjects
Microbiology (medical) ,isoniazid ,Tuberculosis ,medicine.drug_class ,Antibiotics ,lcsh:QR1-502 ,Drug resistance ,Microbiology ,lcsh:Microbiology ,Mycobacterium tuberculosis ,03 medical and health sciences ,Plasmid ,medicine ,Gene ,030304 developmental biology ,Original Research ,0303 health sciences ,drug resistance ,biology ,030306 microbiology ,Isoniazid ,respiratory system ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,medicine.disease ,bacterial infections and mycoses ,Phenotype ,respiratory tract diseases ,katG ,bacteria ,mutation ,medicine.drug - Abstract
Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis, is among the top 10 leading causes of death worldwide. The treatment course for TB is challenging; it requires antibiotic administration for at least 6 months, and bacterial drug resistance makes treatment even more difficult. Understanding the mechanisms of resistance is important for improving treatment. To investigate new mechanisms of isoniazid (INH) resistance, we obtained three INH-resistant (INH-R) M. tuberculosis clinical isolates collected by the Taiwan Centers for Disease Control (TCDC) and sequenced genes known to harbor INH resistance-conferring mutations. Then, the relationship between the mutations and INH resistance of these three INH-R isolates was investigated. Sequencing of the INH-R isolates identified three novel katG mutations resulting in R146P, W341R, and L398P KatG proteins, respectively. To investigate the correlation between the observed INH-R phenotypes of the clinical isolates and these katG mutations, wild-type katG from H37Rv was expressed on a plasmid (pMN437-katG) in the isolates, and their susceptibilities to INH were determined. The plasmid expressing H37Rv katG restored INH susceptibility in the two INH-R isolates encoding the W341R KatG and L398P KatG proteins. In contrast, no phenotypic change was observed in the KatG R146P isolate harboring pMN437-katG. H37Rv isogenic mutant with W341R KatG or L398P KatG was further generated. Both showed resistant to INH. In conclusion, W341R KatG and L398P KatG conferred resistance to INH in M. tuberculosis, whereas R146P KatG did not affect the INH susceptibility of M. tuberculosis.
- Published
- 2020
47. Cetyltrimethylammonium Bromide Disrupts the Mesenchymal Characteristics of HA22T/VGH Cells
- Author
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Chia-Herng, Yue, Chung-Hung, Chen, Wei-Ting, Lee, Tzu-Fen, Su, Ying-Ru, Pan, Yi-Ping, Chen, Fu-Mei, Huang, and Chia-Jen, Lee
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Male ,Carcinoma, Hepatocellular ,Epithelial-Mesenchymal Transition ,Dose-Response Relationship, Drug ,Cell Survival ,Cetrimonium ,TOR Serine-Threonine Kinases ,Liver Neoplasms ,Middle Aged ,Proto-Oncogene Proteins c-met ,Gene Expression Regulation, Neoplastic ,Phosphatidylinositol 3-Kinases ,Cell Movement ,Cell Line, Tumor ,Humans ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Hepatocellular carcinoma (HCC) arises from hepatocytes, and is the most frequently occurring malignancy of primary liver cancer. In this study, we investigated the anti-metastatic effects of the quaternary ammonium compound, cetyltrimethylammonium bromide (CTAB), on HA22T/VGH HCC cells.According to our preliminary data, the effect of CTAB on cell cycle distribution, migration, invasion and the associated protein levels was examined using flow cytometry, wound-healing migration, Matrigel transwell invasion assay and western blotting under sub-lethal concentrations.CTAB treatment of HA22T/VGH cells casued dose-dependent mesenchymal-epithelial transition (MET)-like changes and impaired migration and invasion capabilities. In addition, CTAB reduced the levels of metastasis-related proteins including c-Met, phosphoinositide 3-kinase (PI3K), Akt, mammalian target of rapamycin (mTOR), ribosomal protein S6 kinase (p70S6K), Twist, N-cadherin, and Vimentin. Moreover, pretreatment with hepatocyte growth factor (HGF) rescued CTAB-mediated effects.CTAB exhibited potent anti-EMT and anti-metastatic activities through the inhibition of migration and invasion of HA22T/VGH cells. CTAB interrupted the mesenchymal characteristics of HA22T/VGH cells, which were significantly alleviated by HGF in a dose-dependent manner. CTAB has the potential to evolve as a therapeutic agent for HCC.
- Published
- 2020
48. Assessment of whole-genome sequencing for predicting Mycobacterium tuberculosis drugs resistance
- Author
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C. Chang, Wei-Ting Lee, Mei-Hua Wu, Wan-Hsuan Lin, and Ruwen Jou
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Whole genome sequencing ,Mycobacterium tuberculosis ,Genetics ,Infectious Diseases ,biology ,lcsh:Public aspects of medicine ,Public Health, Environmental and Occupational Health ,lcsh:RC109-216 ,lcsh:RA1-1270 ,General Medicine ,biology.organism_classification ,lcsh:Infectious and parasitic diseases - Published
- 2020
49. Anti-Inflammatory, Antioxidant, and Microbiota-Modulating Effects of Camellia Oil from Camellia brevistyla on Acetic Acid-Induced Colitis in Rats
- Author
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Sheng Yi Chen, Wei Ting Lee, Yu Tang Tung, Hsin Tang Lin, Chun Ching Wu, and Gow-Chin Yen
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0301 basic medicine ,antioxidant ,Physiology ,medicine.drug_class ,colitis ,Camellia brevistyla ,Clinical Biochemistry ,Camellia oleifera ,Pharmacology ,Gut flora ,Biochemistry ,Article ,Anti-inflammatory ,law.invention ,03 medical and health sciences ,Probiotic ,chemistry.chemical_compound ,0302 clinical medicine ,law ,Lactobacillus ,medicine ,Colitis ,Molecular Biology ,Bifidobacterium ,biology ,gut microbiota ,Chemistry ,lcsh:RM1-950 ,food and beverages ,Cell Biology ,medicine.disease ,biology.organism_classification ,Malondialdehyde ,anti-inflammation ,camellia oil ,030104 developmental biology ,lcsh:Therapeutics. Pharmacology ,030220 oncology & carcinogenesis - Abstract
Altering the microbiota by the daily diet is highly associated with improved human health. Studies confirms the gastrointestinal protective and anti-inflammatory effects of camellia oil, however, the benefits in gut microbiota remain unclear. Camellia oils of Camellia oleifera (PCO) and C. brevistyla (TCCO) were used to evaluate probiotic growth in vitro. In addition, the protective effects of camellia oils in the acetic acid (AA)-induced colitis rat model were investigated. In vitro fermentation study showed the proliferation of Lactobacillus spp. and Bifidobacterium spp. from human intestinal microbiota was increased after TCCO treatment. Moreover, the rats pretreated with TCCO exhibited significantly less AA-induced colonic injury and hemorrhage, higher serum immunoglobulin G 1 (IgG 1) levels, lower malondialdehyde levels, and lower inflammatory cytokine production in the colon tissue compared with those in the PCO group. Surprising, the protective effect against acetic acid-induced colitis by TCCO was similar to sulfasalazine (positive control) treatment. Moreover, TCCO increased the richness and diversity of probiotics in gut microbiota. TCCO alleviated AA-induced colitis by modulating gut microbiota, reducing oxidative stress and suppressing inflammatory responses.
- Published
- 2020
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50. Immunomodulatory effect of camellia oil (Camellia oleifera Abel.) on CD19+ B cells enrichment and IL-10 production in BALB/c mice
- Author
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Chia-Ying Lin, Sheng-Yi Chen, Wei-Ting Lee, and Gow-Chin Yen
- Subjects
B cell ,Nutrition and Dietetics ,Traditional medicine ,biology ,Macrophage ,Nutrition. Foods and food supply ,Chemistry ,Camellia oleifera ,food and beverages ,Medicine (miscellaneous) ,biology.organism_classification ,CD19 ,BALB/c ,Immunomodulation ,Interleukin 10 ,CAMELLIA OIL ,biology.protein ,Camellia oil ,TX341-641 ,Cytokine ,Food Science - Abstract
Camellia oil (Camellia oleifera Abel.) has anti-inflammatory, antioxidant, and microbiota-modulating abilities, but little is known about the effects of camellia oil on immune cell immunomodulation. This study aimed to evaluate the immunomodulatory ability of camellia oil. Firstly, oleic acid (77.98%) rich and antioxidant characteristics were found in camellia oil. Besides, camellia oil significantly increased phagocytosis in RAW 264.7 macrophages and primary peritoneal macrophages, respectively. Nitric oxide (NO) production was reduced in camellia oil-treated RAW 264.7 cells and BALB/c mice. Additionally, interleukin-10 (IL-10) was significantly induced by camellia oil treatment in primary splenocytes. Interestingly, IL-10-producing splenic macrophages have been demonstrated to promote B cell proliferation. Noteworthily, this study provided compelling evidence that administration of camellia oil significantly enriched the cluster of differentiation 19 positive (CD19+) B cell population in BALB/c mice. In conclusion, the data demonstrated that oleic acid-rich camellia oil may significantly induce the CD19+ humoral immune response.
- Published
- 2022
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