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The demand for fish protein continues to increase and currently accounts for 17% of total animal protein consumption by humans. About 90% of marine fish stocks are fished at or above maximum sustainable levels, with aquaculture propagating as one of the fastest growing food sectors to address some of this demand. Cell-cultivated seafood production is an alternative approach to produce nutritionally-complete seafood products to meet the growing demand. This cellular aquaculture approach offers a sustainable, climate resilient and ethical biotechnological approach as an alternative to conventional fishing and fish farming. Additional benefits include reduced antibiotic use and the absence of mercury. Cell-cultivated seafood also provides options for the fortification of fish meat with healthier compositions, such as omega-3 fatty acids and other beneficial nutrients through scaffold, media or cell approaches. This review addresses the biomaterials, production processes, tissue engineering approaches, processing, quality, safety, regulatory, and social aspects of cell-cultivated seafood, encompassing where we are today, as well as the road ahead. The goal is to provide a roadmap for the science and technology required to bring cellular aquaculture forward as a mainstream food source.

Paget-Schroetter syndrome (PSS), a rare form of deep vein thrombosis affecting the upper extremity, arises from mechanical compression of the subclavian vein at the thoracic outlet. Typically seen in young, active individuals, it manifests with acute onset of arm pain, swelling, and discoloration. Early diagnosis is crucial to prevent chronic complications such as post-thrombotic syndrome, emphasizing the importance of timely intervention and individualized treatment approaches for improved clinical outcomes. We present a case of PSS manifesting in a young adult with no significant medical history.

Gombang adalah alat tangkap ikan tradisional, memiliki kelemahan konstruksi berupa ketiadaan kantong perangkap dan sayap yang terbatas. Tujuan penelitian adalah 1. Menentukan perbedaan komposisi jenis dan berat ikan hasil tangkapan keempat perlakuan 2. Membuktikan bahwa penambahan kantong, penaju, dan kombinasi keduanya akan memengaruhi komposisi berat hasil tangkapan gombang tanpa mengurangi jenisnya. Metode yang digunakan adalah experimental fishing. Penelitian dilakukan pada bulan Mei-Agustus 2023 di perairan Desa Prapat Tunggal Kabupaten Bengkalis Provinsi Riau. Uji coba penangkapan dilakukan selama 17 trip. Hasil penelitian menunjukkan bahwa gombang kontrol dan perlakuan menangkap 12 jenis ikan yang sama, yaitu udang rebon (Acetes sp.), udang merah (Solenocera depressa), udang harimau (Penaeus monodon), biang (Ilisha sp.), bulu ayam (Thryssa setiostris), tembang (Dussumieria acuta), tenggiri (Scomberomorus brasiliensis), parang-parang (Chirocentrus sp.), gulamah (Johnius trachycephalus), layur (Trichiurus lepturus), nomei (Horpodon neherus), dan sotong (Sepia sp.). Perbedaan perlakuan pada gombang ternyata memberikan pengaruh terhadap hasil tangkapan. Gombang yang dilengkapi kantong dan penaju (P3) mendapatkan berat ikan hasil tangkapan tertinggi mencapai 1.710,82 kg (40,16%), diikuti oleh gombang berkantong P1 seberat 1.158,90 kg (27,20%), gombang berpenaju P2 seberat 824,27 kg (19,35%), dan gombang kontrol P0 seberat 566,36 kg (13,29%).

Abstract The demand for fish protein continues to increase and currently accounts for 17% of total animal protein consumption by humans. About 90% of marine fish stocks are fished at or above maximum sustainable levels, with aquaculture propagating as one of the fastest growing food sectors to address some of this demand. Cell-cultivated seafood production is an alternative approach to produce nutritionally-complete seafood products to meet the growing demand. This cellular aquaculture approach offers a sustainable, climate resilient and ethical biotechnological approach as an alternative to conventional fishing and fish farming. Additional benefits include reduced antibiotic use and the absence of mercury. Cell-cultivated seafood also provides options for the fortification of fish meat with healthier compositions, such as omega-3 fatty acids and other beneficial nutrients through scaffold, media or cell approaches. This review addresses the biomaterials, production processes, tissue engineering approaches, processing, quality, safety, regulatory, and social aspects of cell-cultivated seafood, encompassing where we are today, as well as the road ahead. The goal is to provide a roadmap for the science and technology required to bring cellular aquaculture forward as a mainstream food source.

Kapal Jala Jatuh merupakan kapal cumi terbanyak kedua yang ada di PPN Muara Angke. Aktivitas bongkar merupakan aktivitas utama yang dilakukan setelah kapal berlabuh di dermaga. Aktivitas ini terindikasi memiliki potensi kecelakaan kerja, sehingga perlu adanya identifikasi potensi bahaya pada aktivitas bongkar muat ini. Penelitian ini bertujuan untuk mengidentifikasi aktivitas bongkar muat pada Kapal Jala Jatuh, menghitung persentase konsekuensi bahaya pada setiap aktivitas bongkar muat pada Kapal Jala Jatuh, dan memberikan rekomendasi untuk menjaga keselamatan dan mecegah kecelakaan yang dapat terjadi pada pekerja yang terlibat dalam proses tersebut. Metode yang digunakan dalam penelitian ini adalah observasi dan wawancara kepada kapten, ABK, dan buruh angkut. Analisis yang digunakan berupa analisis deskriptif, dengan mengelompokkan konsekuensi bahaya masing-masing aktivitas berdasarkan AS/NZS 4360: 1999 dimana terdapat lima jenis konsekuensi bahaya. Hasil penelitian menunjukkan bahwa terdapat 71 aktivitas pada kegiatan bongkar muat. Terdapat 8 (11%) aktivitas dikategorikan tidak berbahaya, 40 (56%) aktivitas dikategorikan bahaya ringan, 19 (27%) aktivitas dikategorikan bahaya menengah, 0 (0%) aktivitas dikategorikan bahaya berat, dan 4 (6%) aktivitas dikategorikan bahaya fatal. Perlu adanya perhatian mengenai keselamatan kerja pada aktivitas bongkar muat seperti kelengkapan Alat Pelindung Diri (APD) terutama pada saat aktivitas bongkar muat­, dan juga pengemudi yang bersertifikat guna mencegah terjadinya kecelakaan yang bersifat fatal.

Cancer cachexia is a multifactorial syndrome characterized by progressive weight loss and a disease process that nutritional support cannot reverse. Although progress has been made in preclinical research, there is still a long way to go in translating research findings into clinical practice. One of the main reasons for this is that existing preclinical models do not fully replicate the conditions seen in clinical patients. Therefore, it is important to understand the characteristics of existing preclinical models of cancer cachexia and pay close attention to the latest developments in preclinical models. The main models of cancer cachexia used in current research are allogeneic and xenograft models, genetically engineered mouse models, chemotherapy drug-induced models, Chinese medicine spleen deficiency models, zebrafish and Drosophila models, and cellular models. This review aims to revisit and summarize the commonly used animal models of cancer cachexia by evaluating existing preclinical models, to provide tools and support for translational medicine research.

BackgroundPatient understanding of their care, supported by physician involvement and consistent communication, is key to positive health outcomes. However, patient and care team characteristics can hinder this understanding.ObjectiveWe aimed to assess inpatients' understanding of their care and their perceived receipt of mixed messages, as well as the associated patient, care team, and hospitalization characteristics.DesignWe administered a 30-item survey to inpatients between February 2020 and November 2021 and incorporated other hospitalization data from patients' health records.ParticipantsRandomly selected inpatients at two urban academic hospitals in the USA who were (1) admitted to general medicine services and (2) on or past the third day of their hospitalization.Main measuresOutcome measures include (1) knowledge of main doctor and (2) frequency of mixed messages. Potential predictors included mean notes per day, number of consultants involved in the patient's care, number of unit transfers, number of attending physicians, length of stay, age, sex, insurance type, and primary race.Key resultsA total of 172 patients participated in our survey. Most patients were unaware of their main doctor, an issue related to more daily interactions with care team members. Twenty-three percent of patients reported receiving mixed messages at least sometimes, most often between doctors on the primary team and consulting doctors. However, the likelihood of receiving mixed messages decreased with more daily interactions with care team members.ConclusionsPatients were often unaware of their main doctor, and almost a quarter perceived receiving mixed messages about their care. Future research should examine patients' understanding of different aspects of their care, and the nature of interactions that might improve clarity around who's in charge while simultaneously reducing the receipt of mixed messages.

Background: Antimicrobial resistance is associated with increasing mortality rates and the emergence of multidrug-resistant (MDR) organisms. There is scarcity of data on the short-term impact of Antimicrobial Stewardship Programs (ASP) on antibiotic usage, clinical outcome and MDR organisms’ pattern following the COVID-19 pandemic. This study evaluated the short-term effects of ASP on antibiotic usage, clinical outcomes and MDR organisms’ pattern in the post COVID-19 era. Methods: Conducted at a tertiary academic health center, this observational study involved adult patients (≥18 years) in the general medical unit, treated with oral or intravenous antibiotics from May 1, 2021, to April 30, 2022. The applied ASP strategy was a prospective audit and feedback where a weekly meeting was held to discuss the antimicrobial therapy of admitted patient, after which recommendations were made regarding antimicrobial use. Results: The study included 301 patients with 166 (55.1%) pre-ASP and 135 (44.9%) post-ASP. The median (IQR) age was 69 (55−77) years with 56.1% were female. Antibiotic usage dropped by 25.2% post-ASP. The length of hospital stay (LOS) was longer post-ASP (7 days vs. 7.9 days, p = 0.001), with MDR infections being a significant predictor (OR: 0.486, p

This study deals with the use of finite element analysis to study the response of an RC building that has inclined supporting leads in the foundations. Several RC buildings were studied to evaluate the seismic performance of these buildings under the influence of change in three parameters (the degree of inclination of the foundations (0°, 10°, 20° & 40°), the storey number (8, 10, 12, 16 & 20 storey), and the span length (4, 5 & 6 m)). All buildings were analyzed using the SAP2000 software using the time history analysis method under the influence of the EL-Centro N-S 1940 earthquake acceleration record. The results were shown the values of the base shear, time period and roof displacements decreased by 23%, 33% and 55% respectively, when the degree of foundation inclination of the building increased from 0° to 20°, while these values increased by 68%, 53.28% and 137.6% respectively, when the No. of stories increased by 50%. The increase of base shear due to increase the height of building, also the increase lateral displacements of the buildings. The increases in time period due to decrease the stiffness of RC building and it have more displacements at tip of buildings.

Abstract Background Forensic psychiatry is often associated with long admissions and has a high cost of care. There is little known about factors influencing length of stay (LOS), and no previous systematic review has synthesised the available data. This paper aims to identify factors influencing the LOS in forensic psychiatry hospitals to inform care and interventions that may reduce the length of admissions. Methodology A systematic review was conducted by searching major databases, including PubMed, EMBASE and PsycInfo, from inception until May 2022. Observational studies conducted in forensic hospitals that examined associations between variables of interest and LOS were included. Following data extraction, the Newcastle‒Ottawa Scale was used for quality appraisal. No meta-analysis was conducted due to heterogeneity of information; a quantitative measure to assess the strength of evidence was developed and reported. Results A total of 28 studies met the inclusion criteria out of 1606 citations. A detailed quantitative synthesis was performed using robust criteria. Having committed homicide/attempted homicide, a criminal legal status with restrictions, and a diagnosis of schizophrenia-spectrum disorders were all associated with longer LOS. Higher Global Assessment of Functioning (GAF) scores were associated with a shorter LOS. Conclusion High-quality research examining factors associated with LOS in forensic psychiatry is lacking, and studies are heterogeneous. No modifiable characteristics were identified, and thus, practice recommendations were not made. There is an increasing necessity to understand the factors associated with longer admissions to inform care and increase success in reintegration and rehabilitation. This paper provides recommendations for future research.

Background and Objectives: Urinary tract infections (UTIs), one of the most prevalent bacterial infections, are facing limited treatment options due to escalating concern of antibiotic resistance. Urine cultures significantly help in identification of etiological agents responsible for these infections. Assessment of antibiotic susceptibility patterns of these bacteria aids in tackling the emerging concern of antibiotic resistance and establishment of empirical therapy guidelines. Our aim was to determine various agents responsible for urinary tract infections and to assess their antibiotic susceptibility patterns. Materials and Methods: This cross-sectional study was performed over a period of six months from January 2023 to July 2023 in Department of Microbiology of Pakistan Institute of Medical Sciences (PIMS). Results: Out of 2957 positive samples, Gram negative bacteria were the most prevalent in 1939 (65.6%) samples followed by Gram positive bacteria in 418 (14.1%) and Candida spp. in 269 (9.1%) samples. In gram negative bacteria, Escherichia coli (E. coli) was the most prevalent bacteria isolated from 1070 samples (55.2%) followed by Klebsiella pneumoniae in 397 samples (20.5%). In Gram positive bacteria, Enterococcus spp. was the most common bacteria in 213 samples (51%) followed by Staphylococcus aureus in 120 samples (28.7%). Amikacin was the most sensitive drug (91%) for Gram negative bacteria. Gram positive bacteria were most susceptible to linezolid (97%-100%). Conclusion: The generation of a hospital tailored antibiogram is essential for the effective management of infections and countering antibiotic resistance. By adopting antimicrobial stewardship strategies by deeper understanding of sensitivity patterns, we can effectively combat antibiotic resistance.

Simple and green methods of developing nanoparticles (NPs) have attracted the attention of researchers. Literature on utilising leaf extract to prepare cerium oxide (CeO2 NPs) is scarce. The present study synthesised leaf-mediated-CeO2 NPs to produce nanopowders of controllable sizes for further applications. The study is the first to report the optimised parameters (pH 7, 5 g/150 mL concentration of the leaf extract, and 3 h of reaction time) of procuring CeO2 NPs using Melastoma sp. leaf extract as the capping agent with excellent properties. The absorbance of the NPs suspension obtained in this study was recorded at approximately 252 nm with Ultraviolet–Visible (UV–Vis) Spectroscopy. Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), and Transmission Electron Microscopy (TEM) were also utilised to characterise and confirm the CeO2 NPs prepared. The XRD spectra documented the purity of the NPs at specific diffraction patterns, while TEM revealed the spherical form of the NPs with a particle size of 16 nm. The formation of CeO2 NPs has been confirmed from the FTIR spectra procured, which exhibited a Ce–O peak at 555 nm. Phytochemical screening test and FT-IR analysis of leaf extract revealed the existence of flavonoids, terpenoids, sugars, saponins, quinones, and glycosides. The NPs suspensions of varying concentrations (control, 50, 100, 150, 200, and 250 μg/mL) were prepared and employed for evaluations against Gram-positive and -negative bacteria. Resultantly, CeO2 NPs demonstrated antibacterial activities against both bacteria types. The highest antibacterial activities were recorded against E. coli and K. pneumonia at 1.83 ± 0.137 and 1.83 ± 0.14 mm maximum inhibition zones, respectively, at 250 mg/uL of the NPs.

Despite the critical role of balanced nutrition in crop productivity, the use of potash (K) and zinc (Zn) is not much practiced by Pakistani farmers. The reduced nutrient uptake and crop productivity together increase the costs associated with fertilization and revisit farmers’ confidence in the efficacy and profitability of fertilizers. To address this problem, a field study was conducted in the research area of the MNS-University of Agriculture, Multan, in collaboration with Engro Fertilizers Limited. The research plan consisted of five treatments, including T1 = control (without N, P, K, and Zn fertilizers), T2 = NP in practice (NP at 32–23–0 kg acre-1), T3 = recommended NP (NP at 48–34.5 kg acre-1), T4 = balanced NPK (NP+K at 48–34.5–30 kg acre-1), and T5 = balanced NPK + Zn (NPK+Zn at 48–34.5–30 + 7.5 kg acre-1). Wheat was used as a test crop, and its growth, yield, and physiological and nutritional parameters were studied. The results indicated that NPK+Zn balanced nutrition increased plant height, spike length, photosynthetic rate, water use efficiency, transpiration rate, stomatal conductance, and grain yield by 13%, 15%, 44%, 60%, 63%, 39%, and 78%, respectively, compared with the control. It was found that the combined application of NP, K, and Zn improved the recovery efficiency of applied nutrients, i.e., nitrogen recovery efficiency (NRE) by 230%, phosphorus recovery efficiency (PRE) by 136%, potassium recovery efficiency (KRE) by 135%, and zinc recovery efficiency (ZnRE) by 136% compared to NP-alone application. Agronomic use efficiency of applied fertilizers, such as potassium agronomic use efficiency (KAUE) by 71%, phosphorus agronomic use efficiency (PAUE) by 72%, nitrogen agronomic use efficiency (NAUE) by 70%, and zinc agronomic use efficiency (ZnAUE) by 72%, was observed compared to NP-alone application. The results showed that NPUE, PPUE, NPUE, and ZnPUE were reduced by 5%, 3%, 3%, and 5%, respectively, compared to NP-alone application. Our findings suggest that K and Zn should be made an essential part of wheat nutrition management for higher yield and better quality of produce.

Objective: Doxorubicin is presently a leading antineoplastic drug with promising efficacy. This study was designed to investigate the histological effects of doxorubicin toxicity on rat kidneys and how much protection is provided by Nigella Sativa. Materials & Methods: A randomized controlled trial conducted on thirty adult male wistar rats divided randomly into three equal groups. Group A served as a control. Group B was injected with weekly intraperitoneal injections of doxorubicin at a dose of 3mg/kg b.w. Group C rats received doxorubicin along with nigella sativa at a dose of 1000mg/kg b.w. orally daily. At the end of these interventions, animals were sacrificed and kidneys were removed for the purpose of histological staining. Renal glomerular and tubules related histopathological parameters were assessed qualitatively as mild, moderate & severe. Renal glomerular diameter was digitally measured by microscope. Ethical approval was taken from Ethical Committee, Jinnah Postgraduate Medical Centre (JPMC), Karachi. All the parameters were statistically analyzed. Results: Group B rats’ renal tissue was adversely affected by the drug showing marked necrosis of tubules and the glomeruli along with interstitial cells’ infiltration. Glomerular diameter was also significantly decreased in group B as compared to group A. These histological features in group C rats’ renal tissue were milder and glomerular diameter was close to that of group A. Conclusion: According to our study doxorubicin treatment proved noxious for the renal tissue, both tubules and glomeruli, while nigella sativa significantly competed against these pathological alterations.

The ridgetail white prawn, Exopalaemon carinicauda, has advantages in its rapid growth and strong environmental adaptability, and it is a good species for the biological research of crustaceans. To explore the function of the pcna gene in the ovarian development of crustaceans, we cloned the pcna (Ec-pcna) from E. carinicauda. The open reading frame of the pcna is 786 bp, and it encodes a total of 261 aa. A PCNA/RFC (replication factor C) domain exists at 208–254 aa, and according to a phylogenetic analysis, the amino acid sequence of Ec-pcna has the highest similarity with the pcna gene from Litopenaeus vannamei, and it is clustered into one branch with L. vannamei and Penaeus monodon. The results of qRT-PCR showed that the expression of pcna was highest in the ovarian tissue of E. carinicauda, and it was significantly higher than that of other tissues (p

Despite technological advances, clinical skills are the foundation of medical practice. Taking a comprehensive clinical history, performing physical examinations, and communicating effectively are crucial for high-quality patient care. Medical students need to focus on developing strong clinical skills to ensure they are well-prepared for their careers and adapt to the evolving field of medicine through continual practice and refinement.1 The medical community is worried about declining essential clinical skills among undergraduate medical students.2,3 This editorial aims to analyze the reasons for the decline and suggest evidence-based methods to address the situation. 05885.0258063. The declining standards of clinical skills among medical students can be attributed to the widespread adoption of technology-based learning, which has the potential to diminish the importance of traditional hands-on bedside teaching.4 Technology should enhance, not replace, practical clinical experiences. Practical training improves the clinical abilities, expertise, and self-assurance of medical students.5 Moreover, the growing magnitude and expansion of medical curricula provide difficulties in the development of educational programs without ample time for bedside practical teaching.6,7 There is frequently an excessive focus on remembering facts rather than on cultivating clinical reasoning abilities.8 The reduced patient interaction during medical school, due to increased reliance on simulations and less time in clinical rotations and bedside teaching, hampers the development of clinical skills.9 External factors such as limited time and extensive paperwork may affect the quality of patient encounters. Efficient communication is crucial for building good relationships with patients, obtaining accurate medical histories, and ensuring patient understanding and adherence to treatment.9 Insufficient training in communication skills can result in less-than-ideal interactions between patients and healthcare providers and can also contribute to errors in diagnosis and medical treatment.10 The provision of trained faculty for teaching medical students is an issue worldwide. Insufficient supervision and feedback during clinical rotations hinder the development of essential clinical skills.11 Larger student cohorts reduce the opportunities for individual students to practice and receive personalized instruction in clinical skills.12 Medical education often prioritizes theoretical testing over practical examinations and undervalues communication skills training. Variability in clinical experiences at different medical schools can lead to inconsistencies in skill acquisition. The scarcity of skilled clinicians as role models impacts students’ learning. Economic constraints and modern healthcare pressures limit opportunities for thorough patient interactions and clinical training.13 THE WAY FORWARD Educational institutions should prioritize hands-on clinical experiences throughout the curriculum to improve students’ diagnostic and communication abilities. These experiences should include simulation-based learning, standardized patients, and longitudinal clerkships. Faculty members need support and resources for effective clinical mentorship and development. Reflective practice should be integrated into the curriculum to enhance self-awareness and critical thinking. Open communication and mentorship programs between students and faculty can strengthen medical training and promote continual learning. To address declining clinical proficiency among medical students, institutions can adopt evidence-based approaches including curriculum changes, faculty training, promoting reflective thinking, collaboration among healthcare professionals, and fostering a culture of ongoing learning. Regulatory bodies should ensure adequate time for bedside teaching and appropriate assessment weightage.

Purpose: Cognitive load theory (CLT) is receiving increased recognition in medical education and it was cited as an important theoretical framework for simulation-based medical education. Simulated learning environments can place a high demand on the cognitive resources of the learners, hence, we aimed to design an instructional framework to optimise the total cognitive load imposed on the medical students during their clinical skills training in the clinical skills laboratory. Method: This study is a quasiexperimental post-test design. The sampling technique was purposive sampling, which included year 2 students at the Faculty of Medicine-Suez Canal University population. The study was conducted in the clinical skills and simulation laboratory. The intervention group received a developed instructional design framework based on CLT. The control group learned with the ordinary teaching method without any intervention. The cognitive load was measured using the Cognitive Load Inventory (CLI) immediately after the training session for both groups. Furthermore, students' achievement in the clinical skill laboratory was compared in both groups. Findings: The total cognitive load is lower in the group that received the developed instructional design than that of the control group, and this result was statistically significant. Also, the performance of the intervention group is higher than in the control group. Implications for research and practice: The developed instructional design framework is a potentially useful guide for managing students' cognitive load in the clinical skills training session.

This study focuses on measuring the levels of naturally occurring radioactivity in the soil of Swabi, Khyber Pakhtunkhwa, Pakistan, as well as the associated health hazard. Thirty (30) soil samples were collected from various locations and analyzed for 226Ra, 232Th, and 40K radioactivity levels using a High Purity Germanium detector (HPGe) gamma-ray spectrometer with a photo-peak efficiency of approximately 52.3%. The average values obtained for these radionuclides are 35.6 ± 5.7 Bqkg−1, 47 ± 12.5 Bqkg−1, and 877 ± 153 Bqkg−1, respectively. The level of 232Th is slightly higher and 40K is 2.2 times higher than the internationally recommended limit of 30 Bqkg−1 and 400 Bqkg−1, respectively. Various parameters were calculated based on the results obtained, including Radium Equivalent (Raeq), External Hazard (Hex), Absorbed Dose Rate (D), Annual Gonadal Equivalent Dose (AGDE), Annual Effective Dose Rate, and Excess Lifetime Cancer Risk (ELCR), which are 170.3 ± 24 Bqkg−1, 0.46 ± 0.06 Bqkg−1, 81.4 ± 2.04 nGy h−1, 582 ± 78.08 μSvy−1, 99.8 ± 13.5 μSv Gy−1, and 0.349 ± 0.04, respectively. These values are below the limits recommended by the United Nations Scientific Committee on the Effects of Atomic Radiation (UNSCEAR) in 2002. This study highlights the potential radiation threats associated with natural radioactivity levels in the soil of Swabi and provides valuable information for public health and safety.