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12 results on '"Wahjudi, Paulin N."'

1. UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells

Author

Zhang, Jin, Khvorostov, Ivan, Hong, Jason S, Oktay, Yavuz, Vergnes, Laurent, Nuebel, Esther, Wahjudi, Paulin N, Setoguchi, Kiyoko, Wang, Geng, Do, Anna, Jung, Hea-Jin, McCaffery, J Michael, Kurland, Irwin J, Reue, Karen, Lee, Wai-Nang P, Koehler, Carla M, and Teitell, Michael A

Subjects
Biological Sciences, Biomedical and Clinical Sciences, Information and Computing Sciences, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

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Published
2016

5. UCP 2 regulates energy metabolism and differentiation potential of human pluripotent stem cells

Author

Zhang, Jin, primary, Khvorostov, Ivan, additional, Hong, Jason S, additional, Oktay, Yavuz, additional, Vergnes, Laurent, additional, Nuebel, Esther, additional, Wahjudi, Paulin N, additional, Setoguchi, Kiyoko, additional, Wang, Geng, additional, Do, Anna, additional, Jung, Hea‐Jin, additional, McCaffery, J Michael, additional, Kurland, Irwin J, additional, Reue, Karen, additional, Lee, Wai‐Nang P, additional, Koehler, Carla M, additional, and Teitell, Michael A, additional

Published
2016
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6. UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells

Author

Zhang, Jin, Khvorostov, Ivan, Hong, Jason S, Oktay, Yavuz, Vergnes, Laurent, Nuebel, Esther, Wahjudi, Paulin N, Setoguchi, Kiyoko, Wang, Geng, Do, Anna, Jung, Hea-Jin, McCaffery, J Michael, Kurland, Irwin J, Reue, Karen, Lee, Wai-Nang P, Koehler, Carla M, and Teitell, Michael A

Subjects
Information and Computing Sciences, Biological Sciences, Medical and Health Sciences, Developmental Biology
Abstract

It has been assumed, based largely on morphologic evidence, that human pluripotent stem cells (hPSCs) contain underdeveloped, bioenergetically inactive mitochondria. In contrast, differentiated cells harbour a branched mitochondrial network with oxidative phosphorylation as the main energy source. A role for mitochondria in hPSC bioenergetics and in cell differentiation therefore remains uncertain. Here, we show that hPSCs have functional respiratory complexes that are able to consume O-2 at maximal capacity. Despite this, ATP generation in hPSCs is mainly by glycolysis and ATP is consumed by the F1F0 ATP synthase to partially maintain hPSC mitochondrial membrane potential and cell viability. Uncoupling protein 2 (UCP2) plays a regulating role in hPSC energy metabolism by preventing mitochondrial glucose oxidation and facilitating glycolysis via a substrate shunting mechanism. With early differentiation, hPSC proliferation slows, energy metabolism decreases, and UCP2 is repressed, resulting in decreased glycolysis and maintained or increased mitochondrial glucose oxidation. Ectopic UCP2 expression perturbs this metabolic transition and impairs hPSC differentiation. Overall, hPSCs contain active mitochondria and require UCP2 repression for full differentiation potential. The EMBO Journal (2011) 30, 4860-4873. doi: 10.1038/emboj.2011.401; Published online 15 November 2011

Published
2011

8. UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells

Author

Zhang, Jin, primary, Khvorostov, Ivan, additional, Hong, Jason S, additional, Oktay, Yavuz, additional, Vergnes, Laurent, additional, Nuebel, Esther, additional, Wahjudi, Paulin N, additional, Setoguchi, Kiyoko, additional, Wang, Geng, additional, Do, Anna, additional, Jung, Hea-Jin, additional, McCaffery, J Michael, additional, Kurland, Irwin J, additional, Reue, Karen, additional, Lee, Wai-Nang P, additional, Koehler, Carla M, additional, and Teitell, Michael A, additional

Published
2011
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12. Improvement of metal and tissue adhesion on surface-modified parylene C.

Author

Wahjudi PN, Oh JH, Salman SO, Seabold JA, Rodger DC, Tai YC, and Thompson ME

Subjects
Acrylamides chemistry, Acrylic Resins, Acylation, Animals, Biocompatible Materials chemistry, Biocompatible Materials metabolism, Female, Materials Testing, Molecular Structure, Polymers metabolism, Pregnancy, Rats, Rats, Sprague-Dawley, Surface Properties, Xylenes metabolism, Cell Adhesion physiology, Gold chemistry, Polymers chemistry, Xylenes chemistry
Abstract

A general method for chemical surface functionalization of parylene C [PC, (para-CH2-C6H3Cl-CH2-)n] films is reported. Friedel-Crafts acylation is used to activate the surface of the PC film, and the resulting carbonyl groups are then used to form a range of different organic functional groups to the surface of the parylene film, including alcohol, imine, thiol, phthalimide, amine, and maleimide. The presence of these functional groups on the parylene surface was confirmed by Fourier transform infrared spectroscopy. Static water drop contact angle measurements were also used to demonstrate the changes in hydrophilicity of the PC film surface, consistent with each of the surface modifications. Enhanced metal (gold) adhesion was achieved by anchoring a thiol group onto the acylated surface of PC film. Acylation of parylene with 2-chloropropionyl chloride gave a surface bound chloropropionyl group. Grafting of poly-N-isopropylacrylamide (pNIPAM) onto the chloropropionyl substituted PC film via atom transfer radical polymerization (ATRP) was carried out. The grafted pNIPAM on the parylene surface leads to temperature-dependent cellular tissue adhesion on the PC film., (Copyright 2008 Wiley Periodicals, Inc.)

Published
2009
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