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Objective: This study examined the effects of substituting winged bean tuber steam (WBTS) modified starches for cassava chips (CSC) in the concentrate diet on rumen fermentation, nutrient utilization, and blood metabolites in Thai-native beef cattle. Methods: Four Thai-native bulls were assigned randomly as a 4×4 Latin square design, which represents the amount of CSC replaced with WBTS in the concentrate mixture diets at 0%, 10%, 20%, and 30%. Results: Increasing levels of WBTS replacement for CSC in the concentrate diets had a quadratic effect on total dry matter (DM) intake (p<0.05). Replacement of WBTS at 20% and 30% for CSC did not alter total DM intake compared to 0% WBTS, whereas 10% WBTS replacement could significantly increase total DM intake by 0.41 kg DM/d compared to the control group. In addition, neutral detergent fiber (NDF) digestibility showed a quadratic increase (p<0.05) when CSC was substituted at various levels of WBTS in the concentrate diet (p<0.05). Replacement of CSC with WBTS at 10% and 20% showed higher NDF digestibility when compared to 0% replacement. There was a quadratic increase in blood glucose at 4 h post-feeding, and the average blood glucose value was significantly lower (p<0.01) when substituting CSC with WBTS. Substituting WBTS for CSC at 10% in the concentrate diet showed the highest blood glucose concentration when compared to other treatments. Replacing CSC with WBTS at 10% and 20% shows a higher concentration of C3 than those of other treatments (0% or 30%). The nitrogen (N) intake increased linearly (p<0.05) when substituting WBTS for CSC at all levels in the diet. Additionally, N retention and the ratio of N retention to N intake increased (p<0.05) when substituting WBTS for CSC at 10%, 20%, and 30% compared to 0%. The gross energy intake (GEI), digestible energy intake (DEI), and energy efficiency (DEI/GEI) were quadratically increased when substituted with various levels of WBTS for CSC in the concentrate diet. Conclusion: The findings indicate that substituting 10% of CSC in the concentrate diet with WBTS may be sufficient as an alternative feed resource for improving nutrient utilization and metabolic efficiency in beef cattle diets. [ABSTRACT FROM AUTHOR]

Objective This study examined the effects of substituting winged bean tuber steam (WBTS) modified starches for cassava chips (CSC) in the concentrate diet on rumen fermentation, nutrient utilization, and blood metabolites in Thai-native beef cattle. Methods Four Thai-native bulls were assigned randomly as a 4×4 Latin square design, which represents the amount of CSC replaced with WBTS in the concentrate mixture diets at 0%, 10%, 20%, and 30%. Results Increasing levels of WBTS replacement for CSC in the concentrate diets had a quadratic effect on total dry matter (DM) intake (p

Winged bean (Psophocarpus tetragonolobus (L.) DC.) is one of the tropical legumes commonly grown for vegetable in Indonesia. Winged bean is a kind of plants that growth on vine so that for cultivation it requires stakes or awnings. It is known that paclobutrazol is a growth retardant that acts by inhibiting gibberellin biosynthesis and application of paclobutrazol could make plant become semidwarf or even dwarf. This study was aimed to evaluate the effect of paclobutrazol on growth, development, some phytochemicals content and yield of winged bean plants. This study used a Completely Randomized Design (CRD) with one factor, namely paclobutrazol, which was applied at four different concentrations, namely 0 ppm (control), 25 ppm, 50 ppm, 75 ppm or 100 ppm. Three replicates were made for each treatment. The results showed that paclobutrazol significantly decreased plant height, number of leaves, leaf area, number of pods per plant, pod length, fresh weight of fruit, levels of vitamin C and protein in the pods, but increased the leaf chlorophyll content and stomata density on the abaxial (lower) leaf surfaces. Paclobutrazol showed its effect on accelerating flowering time at a concentration of 50 ppm. [ABSTRACT FROM AUTHOR]

Breeding activities for winged bean plants (Psophocarpus tetragonolobus (L.) DC.) necessitate genetic diversity from local varieties. Identifying winged bean lines adds valuable initial information for shaping policies in plant breeding. Before proceeding with varietal development, it is crucial to establish the ideal type (ideotype) for superior winged bean varieties. The primary objective of this research is to ascertain the idiotype for superior winged bean varieties. The research was conducted in 2023 at Universitas Brawijaya, Indonesia, and involves the evaluation of five improved lines. The initial evaluation focuses on qualitative and quantitative characteristics of ideotype. The second evaluation concerns winged bean seeds' ideal morphological and physiological qualities. The third evaluation assesses winged bean pods' ideal physical and organoleptic preferences. The research outcomes have yielded the ideal morphological characteristics for winged bean varieties. This idiotype will be the foundation for the direction and target of winged bean plant breeding efforts, among the lines evaluated. NSM2 exhibits ideal flowering time and pod length characteristics, and lines PTL and PTL display ideal pod forms. MML and PBL lines have flavors aligned with market preferences regarding organoleptic qualities. These lines will be further improved through crossing to develop ideal varieties.

Urease is an enzyme that has the role to hydrolyzes urea into ammonia and carbon dioxide. Immobilization is one of the most efficient strategies to improve its activity recovery and properties of urease. This research started with the germination of winged beans for 8 days. The winged bean was extracted by grinding using a mortar and pestle and then added with phosphate buffer at pH 7. The solution was homogenized using a stirrer and then centrifuged in cold conditions so that an extract of urease was obtained. Urease extracts were immobilized using a chitosan-supporting matrix. Optimization of the immobilization process of urease extract includes the concentration of chitosan and sodium tripolyphosphate (TPP) and contact time. The obtained was free and immobilized urease activities then tested using the Nessler method and measured using a UV-Vis spectrophotometer with a wavelength of 500 nm. The obtained data were then statistically tested using ANOVA. Urease-chitosan beads were further tested in repeated use and analyzed with SEMEDX (Scanning Electron Microscopy-Energy Dispersive X-ray). The results showed that the optimum conditions for making urease-chitosan beads were a concentration of 4% (w/v), 2.5% (w/v) TPP, and 60 minutes of contact time, resulting in an activity value of 15.076 U/mL, which can be used 5 times with 46% activity from the initial activity. The EDX analysis results after the addition of the enzyme showed atom composition changes leading to increasing carbon and nitrogen contents. The existence of phosphor showed that TPP was a chitosan cross-link compound. [ABSTRACT FROM AUTHOR]

Winged bean (Psophocarpus tetragonolobus L.) is an underutilized legume of the family Fabaceae. We assembled 58,811 unigenes from the publicly available winged bean RNAseq data sets and discovered 4107 perfect SSRs. Seventy-eight of the 166 SSRs amplified into a single band, of which 22 were polymorphic in 79 germplasm accessions of winged bean constituting eight populations from India, Thailand, Nigeria, Indonesia, Taiwan, the Philippines, and Papua New Guinea. We found 60 alleles at the 22 polymorphic SSR loci, with a mean value of 2.73 per locus. With a mean of 0.36, the PIC values for the SSR loci ranged from 0.11 to 0.64. We recorded the maximum genetic diversity in advanced breeding lines (I = 0.67, He = 0.41) and the minimum genetic diversity in the germplasm accessions collected from Manipur (I = 0.44, He = 0.28), a north-eastern Indian state. The AMOVA analysis indicated that within-population variation was significantly higher (85%) than between-population variation (15%). The pairwise Nm values between the populations ranged between 0.69 and 3.41, thereby indicating the varying level of gene flow between them. The analysis of the population structure based on the Bayesian model-based clustering algorithm revealed two distinct groups among the eight populations with different levels of introgression. The fuzzy clustering based on the Manhattan method also identified a similar number of groups, with 72% similarity between the two clustering methods. However, the Neighbour-Joining (NJ)-based clustering grouped all the accessions into four clusters. Nevertheless, all three clustering methods unanimously indicated that most African accessions tended to group, and their leftover members were spread across the hypothetical geographical populations, which indirectly support the African origin of the winged bean. [ABSTRACT FROM AUTHOR]

Winged bean [Psophocarpus tetragonolobus (L.) DC.] is an underutilized legume with the potential to contribute to nutrition and food security globally, particularly in sub-Saharan Africa (SSA). However, more attention needs to be paid to exploiting its full potential due to a lack of adequate knowledge of the existing genetic diversity in the available winged bean germplasm. To bridge this gap, thirty accessions of winged beans sourced from the Gene Bank of the International Institute of Tropical Agriculture (IITA), Ibadan, were evaluated for seed morphometric and selected agro-morphological traits at three agro-ecological zones in Nigeria. The data obtained were subjected to analysis of variance (ANOVA), principal component (PC) analysis, cluster analysis, and correlation analysis. Significant differences (p < 0.05) were observed among the accessions for all measured traits. The first two PCs accounted for 88.2% of the variation observed among the accessions for all measured traits. Accessions were grouped into three clusters based on the agro-morphological traits and three clusters under the multi-spectral imaging (MSI) system. TPt-31 had the highest seed yield per plant, pod weight per plant, and early maturity, while TPt-7 had an extended flowering and maturity period, the highest number of pods per peduncle and pods per plant, as well as the lowest seed yield. These accessions could be a good resource for future winged bean improvement programs. The result also confirmed that the MSI system is an invaluable tool for discriminating among accessions of the same crop species. The findings of this study provide insight into the genetic diversity of winged bean germplasm, which could contribute to improving its yield and quality in SSA and globally.

Abstract The winged bean, Psophocarpus tetragonolobus (L.) D.C., is called the four‐angled bean or Goa bean, which is an underutilized, non‐conventional, and multipurpose tropical legume cultivated in Southeast Asia and Papua New Guinea. The objective of this article was to reveal a comprehensive view on the nutritional properties, biochemical compositions, health benefits, processing, and food applications of winged beans. The average moisture, carbohydrate, protein, fat, crude fiber, and ash content of 138 genotypes of winged bean seeds were found as 9.09 ± 1.73%, 26.81 ± 6.88%, 34.98 ± 4.63%, 18.01 ± 2.27%, 10.24 ± 5.49%, and 4.16 ± 0.41%, respectively. Leaves of winged beans are good sources of vitamins such as vitamin C (14.5–128 mg/100 g), thiamine, riboflavin, niacin, vitamin B6, folate, vitamin A (5240–20,800 IU), and vitamin E. The seed of winged beans is rich in calcium, iron, phosphorus, zinc, and copper. The anti‐nutritional factors, namely, trypsin inhibitor, chymotrypsin inhibitor, phytic acid, saponin, tannin, oxalate, and flatulence saccharides of winged bean, were reported to be in the range of 40–99.5 TIU/mg of protein, 86.4–109.6 CIU/mg of protein, 4.09%–9.96%, 0.6%, 0.77%–0.97%, 0.5%, and 0.04%–0.18%, respectively. This bean can prevent diabetes, cancer, and asthma, boost immunity, and promote women's and men's reproductive health. Winged are transformed into consumable form by processing methods such as soaking, cooking, sprouting, fermentation, roasting, and so forth. This bean is processed into traditional products (curries, soup, pickles, etc.) and contemporary products (milk, tofu, tempeh, etc.). Future research should focus on promoting its utilization as an alternative to soybeans toward meeting global food and nutritional security.

The capability of winged bean to support food and nutrition security in sub-Saharan Africa is recurrently being affected by several constraints, which include a lack of genetic improvement. The dearth of adequate information on the level of available genetic diversity in winged bean germplasm has been a major setback in planning appropriate improvement programs. Fifteen winged bean accessions were assessed for genetic diversity using 10 quantitative traits and 10 simple sequence repeat (SSR) markers. The accessions were laid out in RCBD with three replicates for two growing seasons. Leaf samples were obtained from 10 plants representing each accession for SSR marker genotyping. The accessions exhibited significant (p < 0.05) differences for measured traits. Broad-sense heritability estimates varied from 10.31% for days to first plant maturity to 72.67% for pod weight. Pod weight had a positive and significant correlations with pod length (0.53, p < 0.05), pod width (0.70, p < 0.01), and number of seeds per pod (0.64, p < 0.01). However, the number of seeds per pod was negatively correlated with days to maturity (−0.71, p < 0.01). Number of seeds per pod was positively predicted by pod weight, seed thickness, and days to maturity. Cluster analysis delineated the accessions into two distinct groups. Average number of alleles of 4.2, gene diversity of 0.25, and polymorphic information content of 0.22 were recorded. Analysis of molecular variance revealed intra-accession variation of 95% as compared to inter-accession variation of 5%. Two primary genetic groups were identified and only three accessions, namely TPt-6, TPt-126, and TPt-48, showed genetic purity. The results of this study provide the basis for exploiting the existing diversity for winged bean improvement. [ABSTRACT FROM AUTHOR]

Winged bean [Psophocarpus tetragonolobus (L.) DC.] is an underutilized legume with the potential to contribute to nutrition and food security globally, particularly in sub-Saharan Africa (SSA). However, more attention needs to be paid to exploiting its full potential due to a lack of adequate knowledge of the existing genetic diversity in the available winged bean germplasm. To bridge this gap, thirty accessions of winged beans sourced from the Gene Bank of the International Institute of Tropical Agriculture (IITA), Ibadan, were evaluated for seed morphometric and selected agro-morphological traits at three agro-ecological zones in Nigeria. The data obtained were subjected to analysis of variance (ANOVA), principal component (PC) analysis, cluster analysis, and correlation analysis. Significant differences (p < 0.05) were observed among the accessions for all measured traits. The first two PCs accounted for 88.2% of the variation observed among the accessions for all measured traits. Accessions were grouped into three clusters based on the agro-morphological traits and three clusters under the multi-spectral imaging (MSI) system. TPt-31 had the highest seed yield per plant, pod weight per plant, and early maturity, while TPt-7 had an extended flowering and maturity period, the highest number of pods per peduncle and pods per plant, as well as the lowest seed yield. These accessions could be a good resource for future winged bean improvement programs. The result also confirmed that the MSI system is an invaluable tool for discriminating among accessions of the same crop species. The findings of this study provide insight into the genetic diversity of winged bean germplasm, which could contribute to improving its yield and quality in SSA and globally. [ABSTRACT FROM AUTHOR]

Simple Summary: Cassava chips (CSC) have typically been used as an energy source for ruminant rations in tropical regions due to their high starch content and higher degradability rate in the rumen, which is higher than 90%. However, inconsistencies in production and fluctuations in prices encourage animal nutritionists to search for alternatives to CSC. The introduction of new tuberous plant species may help improve local feed diversity while also reducing feed shortages in particular areas. The winged bean tuber (WBT) has a high proportion of starch that might be able to replace CSC as an energy source. Moreover, it was discovered that steamed WBT might be useful for improving feed efficiency, which could lower rapid starch degradability and maintain rumen pH when compared to the WBT raw material. This research assessed the impact of cassava chips (CSC) and winged bean tubers (WBT) with various starch modification methods on the chemical composition, ruminal degradation, gas production, in vitro degradability, and ruminal fermentation of feed using an in situ and in vitro gas production technique. Experimental treatments were arranged for a 2 × 5 factorial, a completely randomized design with two sources of starch and five levels of modification treatments. Two sources of starch were CSC and WBT, while five modification treatments of starch were: no modification treatment, steam treatment, sodium hydroxide (NaOH) treatment, calcium hydroxide (CaOH2) treatment, and lactic acid (LA) treatment. The starch modification methods with NaOH and CaOH2 increased the ash content (p < 0.05), whereas the crude protein (CP) content was lower after treatment with NaOH (p < 0.05). Steam reduced the soluble fraction (a) and effective dry matter degradability of WBT in situ (p < 0.05). In addition, the WBT steaming methods result in a lower degradation rate constant in situ (p < 0.05). The degradation rate constants for the insoluble fraction (c) in the untreated CSC were higher than those of the other groups. Starch modification with LA reduced in vitro dry matter degradability at 12 and 24 h of incubation (p < 0.05). The starch modification method of the raw material showed the lowest pH value at 4 h (p < 0.05). The source of starch and starch modification methods did not influence the in vitro ammonia nitrogen concentrations, or in vitro volatile fatty acids. In conclusion, compared to the CSC group and untreated treatment, treating WBT with steam might be a more effective strategy for enhancing feed efficiency by decreasing or retarding ruminal starch degradability and maintaining ruminal pH. [ABSTRACT FROM AUTHOR]

The winged bean is an underutilized legume that is adapted to the tropics. It has good prospects as a significant multi-purpose food crop including human nutrition, cattle feed, and environmental protection. However, little research attention has been given to the crop to address the identified constraints, especially low yield in Nigeria. To improve its yield potential, GGE biplot analysis was used to identify high-yielding and stable winged bean genotypes, previously collected from the continent of Asia, and Nigeria for yield improvement. Twenty winged bean genotypes were being evaluated under the rainfed condition at three locations (Ibadan, Ile-Ife, and Kishi) for two years, comprising six environments. The obtained results showed that the seed yield (SY) ranged from 805.61 kg.ha−1 (Ibadan) to 1,096.35 kg.ha−1 (Kishi), with SY of 988.42 kg.ha−1 across the locations. The winged bean reached its first flowering, 50% flowering, 50% podding, and 70% physiological maturity in 74, 80, 93, and 137 days after being planted, respectively across the locations. The GGE biplot analysis showed that the principal component (PC) axes captured 71.5% of the total variation, where PC1 and PC2 were responsible for 36.6% and 34.9%, respectively. Genotype, environment, and their interaction had a significant effect on SY. Environments IB20 and IF20 were adjudged the most ideal environments to discriminate between the genotypes. Genotype Tpt-12 was identified as high-yielding and stable. Tpt-12 would be recommended for commercial farming in southwestern Nigeria. The selected high-yielding winged bean genotypes are hereby recommended as promising parental lines for the grain yield improvement in the winged bean improvement programs. [ABSTRACT FROM AUTHOR]

Excessive leaf production in winged bean may delay the flowering time and reduces the yield, therefore, shoot pruning and additional fertilizer were applied in order to improve yield. The research was carried out at Leuwikopo Experimental Station, IPB University, Bogor from January to July 2022. The study used a randomized complete block design with 2 factors and three replications. The first factor was three types of shoot pruning, namely without shoot pruning, shoot pruning at 15, and 30 cm height. Pruning was conducted at 7 weeks after transplanting (WAT). The second factor was four doses of fertilizers (0, 6.25, 12.5, and 18.75 g of NPK (16-16-16) per plant). The additional fertilizer was given every two weeks from 7 until 15 WAT. The variables observed were the morphophysiological characters, shoot auxin levels, plant nutrient levels, pod yield, pod number, and pod flavonoid content. The results showed that plant’s biomass increased with additional fertilizer, lower relative growth rate and higher auxin content was from 15 cm pruning. The interaction of two factors affected flowering time and flavonoid content. Shoot pruning and 6.25 g NPK delayed flowering by 0.7-1 day. The highest flavonoid content was found in the plant without pruning and additional fertilizer. Plant yields were not significantly affected by treatments. [ABSTRACT FROM AUTHOR]

The Underutilized legume-winged bean (Psophocarpus tetragonolobus (L.) DC.) and its various parts are infested with condensed tannin (CT) or proanthocyanidin (PA). CT has anti-nutritional effect as it adversely affects the digestion of proteins, minerals and vitamin among ruminants and humans. It is also responsible for low protein digestibility and decreased amino acid availability. One of the probable reasons of underutilization of P. tetragonolobus is due to its infestation with CT. Histochemical staining of various tissues of P. tetragonolobus with dimethylcinnmaldehyde (DMACA) developed a deep-blue colour indicating the presence of polyphenolic condensed tannin. Structural monomeric unit catechin and epi-catechin were reported to be responsible for biosynthesis of CT in P. tetragonolobus. The enzyme anthocyanidin synthase (ANS) and its corresponding transcripts were identified and phylogenetically mapped. The transcript was subjected to virus-induced gene silencing (VIGS) through agro-infiltration in P. tetragonolobus for reducing the CT-content. The WbANS-VIGS induced P. tetragonolobus resulted in four-fold decrease of CT as compared to the control P. tetragonolobus. A decrease of 73% of CT level was reported in VIGS silenced Wb-ANS line of P. tetragonolobus. This study resulted and confirmed that, the silencing of (ANS) gene in P. tetragonolobus has a regulatory effect on the condensed tannin biosynthesis. This study will pave way for further manipulation of ANS enzyme for reducing the biosynthesis of the anti-nutrient CT. Reducing the CT content will make this underutilized legume more acceptable. [ABSTRACT FROM AUTHOR]

Winged bean [Psophocarpus tetragonolobus (L.) DC.] is a vegetable legume crop. The center of origin, diversity and domestication of this crop are not known. In this study, we assessed the genetic diversity and population structure of 457 accessions of winged bean collected from six geographical regions (North, Northeast, East, West, and central, and South) in Thailand using 14 simple sequence repeat (SSR) markers. In total, the SSR markers detected only 55 alleles with an average of 3.9 alleles per locus. Observed heterozygosity was relatively high (0.15) and overall gene diversity was moderate (0.487). Gene diversity, allelic richness and observed heterozygosity in the six regions were comparable, while the estimated out-crossing rate was relatively high (16.4%). STRUCTURE analysis grouped the 457 winged bean accessions into three sub-populations. Neighbor-joining (NJ) analysis grouped all the accessions into two major clusters. Genetic groups identified by both STRUCTURE analysis and NJ analysis were unrelated to geographical origins. Principal coordinate analysis revealed no clear clustering of the winged bean accessions. Although genetic groups were not unrelated to geographical origins, most of the winged bean accessions with long pods (30 cm or higher in length) or having purple seed coats or purple young pods were grouped together. This suggested that the winged beans with long pods or with purple seed or purple young pods may have a single origin. Altogether, these results demonstrated that the genetic diversity of winged bean in Thailand was moderate with high genetic admixture. We argue that the high genetic admixture of the winged bean in Thailand is due to seed migration and relatively high outcrossing rate.

Winged bean (Psophocarpus tetragonolobus (L.) DC.) is an underexploited tropical legume whose tuberous-roots have 12–19% proteins. The species has excellent drought tolerance ability thus making it a prospective climate-smart future crop. However, the underlying defense mechanism(s) of the species against the drought stress are not yet understood. Therefore, we studied the role of root- tuber proteins and metabolites in providing drought stress resistance to winged bean. We identified and characterized the relevant proteins and their corresponding peptides, and validated the responsible transcripts in root-tubers of P. tetragonolobus through qRT-PCR. Total proteome-analysis of root-tubers resulted in identification of 2678 proteins belonging to 154 families. Of these, 96% of proteins are associated with known biological activities. Heat-shock proteins (Hsps) of 12–42 kDa were the most abundant protein families and constituted 13% of the total identified proteins in the root-tuber. Metabolite analysis of the root-tuber revealed the prevalence of abiotic stress tolerant amino acids and compounds such as dimethyl amide, hydroxylamine hydrochloride, trimethylamine, cyclopropylamine, α-aminopropionitrile and cyclohexylamine. The other related metabolites present were naphthalene, benzyl alcohol, L- rhamnose, propanol and aromatic aldehydes. The presence of several Hsps, aminoacids, and specific metabolites with known function of stress-amelioration/anti-oxidant activities present in the root-tubers explains the adaptation success of winged bean against drought stress.

The capability of winged bean to support food and nutrition security in sub-Saharan Africa is recurrently being affected by several constraints, which include a lack of genetic improvement. The dearth of adequate information on the level of available genetic diversity in winged bean germplasm has been a major setback in planning appropriate improvement programs. Fifteen winged bean accessions were assessed for genetic diversity using 10 quantitative traits and 10 simple sequence repeat (SSR) markers. The accessions were laid out in RCBD with three replicates for two growing seasons. Leaf samples were obtained from 10 plants representing each accession for SSR marker genotyping. The accessions exhibited significant (p < 0.05) differences for measured traits. Broad-sense heritability estimates varied from 10.31% for days to first plant maturity to 72.67% for pod weight. Pod weight had a positive and significant correlations with pod length (0.53, p < 0.05), pod width (0.70, p < 0.01), and number of seeds per pod (0.64, p < 0.01). However, the number of seeds per pod was negatively correlated with days to maturity (−0.71, p < 0.01). Number of seeds per pod was positively predicted by pod weight, seed thickness, and days to maturity. Cluster analysis delineated the accessions into two distinct groups. Average number of alleles of 4.2, gene diversity of 0.25, and polymorphic information content of 0.22 were recorded. Analysis of molecular variance revealed intra-accession variation of 95% as compared to inter-accession variation of 5%. Two primary genetic groups were identified and only three accessions, namely TPt-6, TPt-126, and TPt-48, showed genetic purity. The results of this study provide the basis for exploiting the existing diversity for winged bean improvement.

This research assessed the impact of cassava chips (CSC) and winged bean tubers (WBT) with various starch modification methods on the chemical composition, ruminal degradation, gas production, in vitro degradability, and ruminal fermentation of feed using an in situ and in vitro gas production technique. Experimental treatments were arranged for a 2 × 5 factorial, a completely randomized design with two sources of starch and five levels of modification treatments. Two sources of starch were CSC and WBT, while five modification treatments of starch were: no modification treatment, steam treatment, sodium hydroxide (NaOH) treatment, calcium hydroxide (CaOH2) treatment, and lactic acid (LA) treatment. The starch modification methods with NaOH and CaOH2 increased the ash content (p < 0.05), whereas the crude protein (CP) content was lower after treatment with NaOH (p < 0.05). Steam reduced the soluble fraction (a) and effective dry matter degradability of WBT in situ (p < 0.05). In addition, the WBT steaming methods result in a lower degradation rate constant in situ (p < 0.05). The degradation rate constants for the insoluble fraction (c) in the untreated CSC were higher than those of the other groups. Starch modification with LA reduced in vitro dry matter degradability at 12 and 24 h of incubation (p < 0.05). The starch modification method of the raw material showed the lowest pH value at 4 h (p < 0.05). The source of starch and starch modification methods did not influence the in vitro ammonia nitrogen concentrations, or in vitro volatile fatty acids. In conclusion, compared to the CSC group and untreated treatment, treating WBT with steam might be a more effective strategy for enhancing feed efficiency by decreasing or retarding ruminal starch degradability and maintaining ruminal pH.

Winged bean [Psophocarpus tetragonolobus (L.) DC.] (2n = 2× = 18) is a tropical legume crop with multipurpose usages. Recently, the winged bean has regained attention from scientists as a food protein source. Currently, there is no breeding program for winged bean cultivars. All winged bean cultivars are landraces or selections from landraces. Molecular markers and genetic linkage maps are pre-requisites for molecular plant breeding. The aim of this study was to develop a high-density linkage map and identify quantitative trait loci (QTLs) for pod and seed-related traits of the winged bean. An F2 population of 86 plants was developed from a cross between winged bean accessions W054 and TPT9 showing contrasting pod length, and pod, flower and seed colors. A genetic linkage map of 1384 single nucleotide polymorphism (SNP) markers generated from restriction site-associated DNA sequencing was constructed. The map resolved nine haploid chromosomes of the winged bean and spanned the cumulative length of 4552.8 cM with the number of SNPs per linkage ranging from 36 to 218 with an average of 153.78. QTL analysis in the F2 population revealed 31 QTLs controlling pod length, pod color, pod anthocyanin content, flower color, and seed color. The number of QTLs per trait varied between 1 (seed length) to 7 (banner color). Interestingly, the major QTLs for pod color, anthocyanin content, and calyx color, and for seed color and flower wing color were located at the same position. The high-density linkage map QTLs reported in this study will be useful for molecular breeding of winged beans. [ABSTRACT FROM AUTHOR]

In Indonesia, the utilization of winged bean seeds as a food source is very limited. Currently there is inadequate information on the characteristics of the seeds, especially the chemical properties associated with its maturity. This research aimed to analyze the chemical properties of winged bean obtained from different harvesting time. Three different harvesting times were investigated, i.e. eight (K1), twelve (K2), and as six (6) weeks after the first flowering stage as a control. K1 and K2 were dried at 40°C (24 h) to mimic the conventional preparation of beans practiced in Indonesia, while K3 was unripe seeds commonly consumed fresh thus it is analyzed as fresh seeds. K1 and K2 have water content between 12.3-13.0% (wb), ash content 4.7-4.8% (db), lipid content 13.4-15.4% (db), protein content 38.9-40.7% (db), carbo-hydrate content 40.8-41.0% (db), total phenolic content 7.6 and 5.3 mg GAE/g (db), antioxidant activity (IC50) 558.3 and 511.1 µg/mL, starch content 25.6-29.1%, reducing sugar content 1.3-1.7 mg/g. Mean-while, the unripe winged bean seeds (K3) has water content of 75.5% (wb), ash content 5.0% (db), protein content 19.6% (db), carbohydrate content 68.4% (db), total phenolic content of 59.4 mg GAE/g (db), anti-oxidant activity (IC50) 485.6 µg/mL, starch content 7.2% and reducing sugar 5.4 mg/g. Based on these che-mical properties and time efficiency, harvesting winged bean at 8 weeks (K1) was sufficient to produce winged bean potential as protein source, as well as a potential functional foods with good antioxidant acti-vity, total phenolic content, low starch and reducing sugar.

ABSTRACT: An experiment was conducted to investigate the effects of different shading regimes [i.e., 60% (heavy), 30% (moderate), and 0% (control)] on 25 traits associated with the morphological features, photosynthetic gas exchange and agronomic characteristics of winged bean (Psophocarpus tetragonolobus), an underutilized protein-rich legume from the tropics. Collectively, 80% of the studied variables displayed significant differences (P

Winged bean [Psophocarpus tetragonolobus (L.) DC.] (2n = 2× = 18) is a tropical legume crop with multipurpose usages. Recently, the winged bean has regained attention from scientists as a food protein source. Currently, there is no breeding program for winged bean cultivars. All winged bean cultivars are landraces or selections from landraces. Molecular markers and genetic linkage maps are pre-requisites for molecular plant breeding. The aim of this study was to develop a high-density linkage map and identify quantitative trait loci (QTLs) for pod and seed-related traits of the winged bean. An F2 population of 86 plants was developed from a cross between winged bean accessions W054 and TPT9 showing contrasting pod length, and pod, flower and seed colors. A genetic linkage map of 1384 single nucleotide polymorphism (SNP) markers generated from restriction site-associated DNA sequencing was constructed. The map resolved nine haploid chromosomes of the winged bean and spanned the cumulative length of 4552.8 cM with the number of SNPs per linkage ranging from 36 to 218 with an average of 153.78. QTL analysis in the F2 population revealed 31 QTLs controlling pod length, pod color, pod anthocyanin content, flower color, and seed color. The number of QTLs per trait varied between 1 (seed length) to 7 (banner color). Interestingly, the major QTLs for pod color, anthocyanin content, and calyx color, and for seed color and flower wing color were located at the same position. The high-density linkage map QTLs reported in this study will be useful for molecular breeding of winged beans.

Psophocarpus tetragonolobus (L.) DC., commonly known as winged bean has been described as a wonder legume in the sense that virtually every part of the plant is edible and comprises a rich source of good quality protein. The present investigation was carried out to find out the effect of chemical mutagen such as Ethyl Methane Sulphonate (EMS) in M2 and M3 generations on yield contributing traits of winged bean. The different characters studied related to yield includes; number of pods per plant, pod length, number of seeds per pod and hundred seed weight. The mutagen EMS have shown negative and positive shifts in mean values for yield contributing traits. The present investigation therefore, has provided significant base for selection and improvement of winged bean for yield contributing traits. [ABSTRACT FROM AUTHOR]

An experiment was conducted to investigate the effects of different shading regimes [i.e., 60% (heavy), 30% (moderate), and 0% (control)] on 25 traits associated with the morphological features, photosynthetic gas exchange and agronomic characteristics of winged bean (Psophocarpus tetragonolobus), an underutilized protein-rich legume from the tropics. Collectively, 80% of the studied variables displayed significant differences (P<0.05) between at least two shade treatments. Shading generally showed most pronounced effect on the physiological traits of the legume, whereby the stomatal conductance, photosynthetic and transpiration rate differed significantly among plants for all treatments. The non-shaded plants were observed to have superior growth and physiological responses than the shaded plants. Interestingly, the moderately shaded plants exhibited the highest yield per plant, which significantly differed from the non-shaded and heavily shaded plants. This indicated that winged bean can adapt to partial canopy cover, making it a potential nitrogen-fixing cash crop which can be planted together with fruit or oil trees in commercial plantations. [ABSTRACT FROM AUTHOR]

Winged beans (Psophocarpus tetragonolobus (L.) DC.) are grown as a vegetable legume crop in Thailand. All parts of the plant, including pods, seeds, leaves, flowers, and tubers are edible and are rich in protein and nutrients. Although the major consumption of winged bean is based on pod and tuber yields, only the people of Myanmar and Indonesia utilize winged bean tubers as food materials. The usefulness of the winged bean as an alternative crop for staple food and feed can shed some light on the impact of winged bean. Therefore, the evaluation of the dual purpose of the winged bean based on pod tuber yields is the objective of this study. In this study, ten-winged bean accessions—six accessions obtained from introduced sources and four accessions obtained from local Thai varieties—were laid out in randomized complete block design (RCBD) with three replications at the Agronomy Field Crop Station, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand from September 2019 to April 2020 and from October 2020 to April 2021. Data, including total pod weight, number of pods, pod length, 10-pod weight, and tuber weight were recorded, and the proximate nutrient and mineral contents in the tubers were also determined. The results revealed that the principal effects of year (Y) and genotype (G) were significant for total pod weight and the number of pods. Moreover, the Y × G interactions were principal effects upon the total pod weights and tuber weights. The results indicated that superior genotype and appropriate environmental conditions are key elements in successful winged bean production for both pod and tuber yields. The winged bean accessions W099 and W018 were consistent in both experimental years for pod and tuber yields at 23.6 and 18.36 T/ha and 15.20 and 15.5 T/ha, respectively. Each accession also proved high in tuber protein content at 20.92% and 21.04%, respectively, as well as significant in fiber, energy, and minerals. The results suggest that the winged bean accessions W099 and W018 can be used for dual-purpose winged bean production in Thailand.

Psophocarpus tetragonolobus has long been used in traditional medicine and cuisine. In this study, Psophocarpus tetragonolobus extracts were isolated by maceration and ultrasound-assisted extraction and were evaluated for their antioxidant and anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The obtained results show that both extracts (maceration and ultrasound) were rich in bioactive molecules and exerted substantial antioxidant and anti-inflammatory effects. The P. tetragonolobus extracts’ treatment in LPS-stimulated RAW264.7 macrophages resulted in a significant downregulation of the expressions of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β mRNA. In addition, the P. tetragonolobus extracts’ treatment attenuated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression. Our observations indicate that there is no significant difference between the two studied extracts of P. tetragonolobus in terms of biological properties (specifically, antioxidant and anti-inflammatory effects. Regardless of the extraction method, P. tetragonolobus could be used for treating diseases related to oxidative stress and inflammatory reactions.

Protein content of winged bean is almost the same as soybean, but the beany flavor is more poweful than soybean. Therefore the protein of winged bean was isolated prior to use as raw material of yogurt. This research was aimed to determine the potency of hypocholestrolemic activity of yogurt protein isolate of winged bean through in vivo bioassay by using Sprague Dawley male rats. The treatments of the research were yogurt feed treatment with concentration of yogurt 0 (standard feed without yogurt as a control), 2, and 4 g yogurt/day as low and high concentration treatment respectively for 4th weeks after hypercholesterol feed treatment for 1 week. The blood lipid profile of rats, including triglyceride, cholesterol total, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) cholesterol were analysed on the 2nd and 4th weeks for the yogurt feed treatment while for before yogurt feed treatment, the evaluation were based on the adaptation phase and the 1st week for hypercholesterol phase. The result of this research showed that the blood triglyceride, cholesterol total, LDL increased, and the blood HDL decreased in hypercholesterol phase before yogurt feed treatment. The potency of hypocholestrolemic of yogurt from protein isolate of winged bean was shown by the decreasing of blood triglyceride, cholesterol total, LDL and increasing the HDL cholesterol after the yogurt feed treatment with low and high concentration. That indicated that yogurt that was made of protein isolate of winged bean could reduced cholesterol. ABSTRAK Biji kecipir memiliki kadar protein yang hampir sama dengan kedelai, namun bau langunya lebih tajam daripada kedelai, sehingga perlu diisolasi proteinnya sebelum digunakan sebagai bahan baku yogurt. Tujuan penelitian ini adalah menentukan potensi hipokolesterolemik yogurt isolat proteun biji kecipir melalui uji biologis in vivo menggunakan tikus jantan Sprague Dawley. Perlakuan penelitian ini adalah perlakuan pakan yogurt dengan konsentrasi 0 (pakan standar tanpa penambahan yogurt sebagai kontrol), 2, dan 4 g yogurt/hari berturut-turut sebagai perlakuan konsentrasi rendah dan tinggi selama 4 minggu perlakuan pakan yogurt sesudah pemberian pakan hiperkolesterol selama 1 minggu. Profil lipida darah tikus meliputi kadar trigliserida, total kolesterol, kolesterol High Density Lipoprotein (HDL), dan Low Density Lipoprotein (LDL) dianalisis pada minggu ke 2 dan 4 minggu selama perlakuan pakan yogurt dan sebelum perlakuan pakan yogurt yaitu pada fase pemeliharaan adaptasi dan 1 minggu pada fase pemeliharan hiperkolesterol. Hasil penelitian ini menunjukkan bahwa trigliserida, total kolesterol, dan kolesterol LDL meningkat dan kolesterol HDL menurun selama fase pemberian pakan hiperkolesterol sebelum perlakuan pakan yogurt. Potensi hipokolesterol yogurt isolat protein biji kecipir ditunjukkan dengan penurunan trigliserida, total kolesterol, dan kolesterol LDL, serta peningkatan kolesterol HDL sesudah perlakuan pakan yogurt dengan konsentrasi rendah maupun tinggi. Hal tersebut mengindikasikan bahwa yogurt isolat protein biji kecipir mampu menurunkan kolesterol.

Winged bean (Fabaceae) is a tropical crop that has high nutrient content, and almost all parts of the plant are edible. The research aimed to evaluate the growth and production of winged bean in an intercropping system with tomato. The study was conducted at the Cikabayan Experimental Field of Bogor Agricultural University, Bogor, Indonesia, in a randomized block design with three replications during the rainy season of December 2017 to June 2018. Winged beans were planted in high and low population densities and intercropped with three tomato genotypes, “Tora”, F70030081-12-16-3 and “Apel Belgia”. Monoculture winged bean was assigned as control. Tomatoes were planted between the rows of the winged beans in the intercropping treatment. The results showed that the growth of winged bean in monoculture and intercropping systems was not significantly different. The photosynthetic activity of winged bean at three to five weeks after planting remained high, indicating that the vegetative growth was optimal in both systems. Intercropping increased the winged bean number of pods per plant by 12.66-19.52% compared to monoculture, irrespective of population density. Therefore, winged bean could be considered as suitable to grow in intercropping systems.

In Indonesia, the utilization of winged bean seeds as a food source is very limited. Currently there is inadequate information on the characteristics of the seeds, especially the chemical properties associated with its maturity. This research aimed to analyze the chemical properties of winged bean obtained from different harvesting time. Three different harvesting times were investigated, i.e. eight (K1), twelve (K2), and as six (6) weeks after the first flowering stage as a control. K1 and K2 were dried at 40°C (24 h) to mimic the conventional preparation of beans practiced in Indonesia, while K3 was unripe seeds commonly consumed fresh thus it is analyzed as fresh seeds. K1 and K2 have water content between 12.3-13.0% (wb), ash content 4.7-4.8% (db), lipid content 13.4-15.4% (db), protein content 38.9-40.7% (db), carbohydrate content 40.8-41.0% (db), total phenolic content 7.6 and 5.3 mg GAE/g (db), antioxidant activity (IC50) 558.3 and 511.1 µg/mL, starch content 25.6-29.1%, reducing sugar content 1.3-1.7 mg/g. Meanwhile, the unripe winged bean seeds (K3) has water content of 75.5% (wb), ash content 5.0% (db), protein content 19.6% (db), carbohydrate content 68.4% (db), total phenolic content of 59.4 mg GAE/g (db), antioxidant activity (IC50) 485.6 µg/mL, starch content 7.2% and reducing sugar 5.4 mg/g. Based on these chemical properties and time efficiency, harvesting winged bean at 8 weeks (K1) was sufficient to produce winged bean potential as protein source, as well as a potential functional foods with good antioxidant activity, total phenolic content, low starch and reducing sugar. [ABSTRACT FROM AUTHOR]

The genetic diversity among ninety five accessions of winged bean (Psophocarpus tetragonolobus (L.) DC.) belonging to six countries of African and Asiatic origin was detected by employing amplified fragment length polymorphism (AFLP) and internal transcribed spacer (ITS) of nuclear ribosomal DNA markers. A medium to low-level of genetic diversity exists among the accessions of P. tetragonolobus. Association between the AFLP markers and flower, pod and seed traits: days to 50% flowering (DFW), pod length (PDL), pod width (PDW), green pod length (GPL), number of pods per plant (PDSP), number of seeds per pod (SDPD), 100 seed weight (SWT) and seed-oil content (SOC) was carried out by employing the mixed linear model (MLM) based kinship matrix (K-model). Traits like SOC and PDW were found to be strongly associated with AFLP markers. Population structure analysis among the genotypes identified five discrete sub-populations among the studied African and Asiatic lines of P. tetragonolobus. [ABSTRACT FROM AUTHOR]

Winged bean being a minor pulse crop has been less focused for research purposes. However, seeing at its enormous potential as a nutrient-rich crop, a study was conducted at ICAR-IIVR, Varanasi to encourage this underutilized legume crop. Nineteen winged bean genotypes were evaluated for their antioxidant potentiality and phenol and flavonoids content. Two chemometric techniques (PCA and AHC) were implied to find out the pattern of variation available for antioxidant potentiality as well as to find out the best performing genotypes. The phenol content ranged from 48.4 to 143.5 (maximum in AMBIKA-II-I) mg GAE/100 g FW, and total flavonoids content ranged from 9.1 to 37.0 mg CE/100G FW (maximum in MWBS-16-26). In both DPPH and TEAC assay, hydrophilic antioxidant activity was towards higher side in AMBIKA-13-4 B (5.02 μmol TE/g FW and 3.06 μmol TE/g FW respectively). The genotypes AMBIKAWB-II-I, AMBIKA-13-4 B and MWBS-16-26 were identified superior based on their overall total phenolics, total flavonoids and antioxidant activities coupled with chemometric analysis. • An attempt was made to popularize winged bean (an underutilized crop) by studying its nutritional potentiality. • Wide range of diversity was found for phenol and flavonoid content. • Chemometric analysis further determined the pattern of grouping among the studied genotypes for high, low and intermediate performance. • Potential germplasm lines were identified through antioxidant assays. • The research findings suggest that presence or absence of the bioactive compounds and their content exert great role in influencing the antioxidant potential of different genotypes. [ABSTRACT FROM AUTHOR]

Winged bean (Psophocarpus tetragonolobus) is a promising source of oil and proteins. One way to utilize winged bean fruit is to process into pickle product. The objective of the present study was to evaluate different variables influencing the fermentation of winged bean such as concentration of CaCl2, temperature and time for blanching, sugar supplementation and effect of ratio Lactobacillus plantarum to the antioxidant of fermented winged bean. Results showed that winged bean should be blanched in hot water at 95oC in 20 sec with the present of 4.0% CaCl2. Moreover, the fermentation process for pickle winged bean had the best antioxidant by addition of 15% sugar, L. plantarum at ratio 3.0 x 108 cells/ml. Blanching and fermentation had significantly affected the antioxidant capacity and firmness of pickle winged bean. After 15th day of pickling, the antioxidant value of all winged beans was increased significantly. The pickle winged bean had a high content of antioxidant as well as firmness appropriated for daily consumption. [ABSTRACT FROM AUTHOR]

Abstract Most margarine and bakery fats are produced from hydrogenated oils making the ingestion of large amounts of trans -fatty acids inevitable with consequential health hazards. Taking advantage of its high solid fat content (15%), winged bean oil-based margarine blends (F320 & F322) were prepared from wing bean oil (WBO), palm stearin (PS) and palm olein (PO) as follows (w/w): F320 (48.5%PS:1.5%PO:50%WBO) and F322 (1.5%PS:48.5%PO: 50%WBO). The blends were tested for textural, thermal properties and crystal growth pattern and polymorphic characteristics. Results showed F320 exhibited harder texture, had crystal morphology with size ranges of 1.0–1.60 μm (50%), 1.61–2.20 μm (23.38%), 2.21–3.40 μm (16.50%) and 3.41–5.20 μm (6.18%), evenly distributed resembling βʹ polymorphs, whereas F322 exhibited soft texture, and polymorphs with protruding needle-like crystals with size ranges of 1–1.60 μm (48.25%), 1.61–2.20 μm (23.96%), 2.21–3.40 μm (16.84%) and 3.41–5.20 μm (6.39%), unevenly distributed. Blend F320 thus showed good physicochemical properties as multi-purpose for use as table and bakery margarine especially in tropical climates. Both blends showed low atherogenic and thrombogenic indexes and thus low risk of CHD. This study further offer insight into the potential use of underutilized oilseed crop resources for making specialty and structured fats for the betterment of human life. Highlights • Zero-trans-fatty acids margarine blends were formulated and produced using winged bean oil as base. • Underutilized winged bean oil with high SFC is potential fat for making trans-fatty acid free margarines. • Blend F320 showed good textural properties for use as multi-purpose margarine in tropical countries. • The trans-fat free margarine blends exhibited low atherogenic and thrombogenic indexes. [ABSTRACT FROM AUTHOR]

Natural products, particularly those extracted from plants, have been used as therapy for different diseases for thousands of years. The first written records on the plants used in natural medicine, referred to as “medicinal plants”, go back to about 2600 BC. A thorough and complete understanding of medicinal plants encompasses a multiplex of overlapping and integrated sciences such as botany, pharmacognosy, chemistry, enzymology and genetics. Psophocarpus tetragonolobus, a member of Fabaceae family also called winged bean, is a perennial herbaceous plant characterized by its tuberous roots and its winged pod twinning and a perennial legume rich in proteins, oils, vitamins and carbohydrates. Besides nutrients, winged bean also contains bioactive compounds that have therapeutic activities like anti-oxidant, anti-inflammatory, antinociceptive, antibacterial, antifungal, antiproliferative and cytotoxic activity, a few of which already been reported. This plant can also be used as a medicinal plant for future benefits. With this concept in mind, the present review is designed to shed the light on the interests in the various phytochemicals and pharmacological pharmacognostical aspects of Psophocarpus tetragonolobus.