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The experiment aimed to explore the effects of honeysuckle, skullcap and mulberry leaves crude extract on the production performance, egg quality, slaughter performance, and meat quality of laying hens in the late laying period. A total of 4 320 six hundred and seventy-day-old Dawu Golden Phoenix laying hens with similar production performance and good health were selected and randomly divided into two groups, with six replicates in each group and 360 hens in each replicate. The control group was fed basic diet and normal drinking water. The experimental group added 0.3% honeysuckle, skullcap and mulberry leaves crude extract to drinking water. The test period was 30 days. The results showed that compared to the control group, the experimental group had a significant increase in the number of normal eggs, total egg production, total weight of normal eggs, average weight of normal eggs, egg production rate, and the number of small yellow follicles (P<0.05), the proportion of abnormal eggs, average number of spotted eggs, average number of eggs with calcium salt deposits, average number of wrinkled eggs, the ratio of spotted eggs, and the ratio of eggs with calcium salt deposits were significantly reduced (P<0.05). Compared to the control group, the pH value of the pectoral muscle, the pH value of the leg muscle, the water-holding capacity of the leg muscle, and the a* and b* values of the leg muscle in the experimental group were all significantly increased (P<0.05), the L* values of both the pectoral and leg muscles in the experimental group were significantly decreased (P<0.05). The study indicates that adding honeysuckle, skullcap and mulberry crude extract to the drinking water of laying hens in the late laying period can effectively increase the egg production performance, improve meat quality and serum biochemical indices. [ABSTRACT FROM AUTHOR]

The experiment was conducted to investigate the effect of adding compound probiotics (Clostridium butyricum + Bacillus subtilis) in different rearing modes on intestinal morphology and bacterial community of Guangxi Sanhuang chickens. 33 000 49-day-old Guangxi Sanhuang chickens (half male and female) were divided into two modes of cage rearing and free range rearing. In cage rearing mode, 3 000 chickens were randomly divided into three groups, namely LA group (basal diet + Clostridium butyricum), LB group (basal diet + compound probiotics) and LC group (basal diet), with five replicates in each group and 200 chickens in each replicate (half male and female). In free-range rearing mode, 30 000 chickens were randomly divided into three groups, SA group (Clostridium butyricum), SB group (compound probiotics) and SC group (basal diet), with five replicates in each group and 2 000 chickens in each replicate (half male and female). The experiment lasted for 30 d. The results showed that under different feeding modes, the addition of compound probiotics in the diet had no significant effect on the growth performance of Guangxi Sanhuang chickens (P>0.05). After cage rearing for 80 days, the height of jejunum villus in LB group was significantly higher than that in LB group and LC group at 49 days (P<0.05). After cage rearing for 80 days, the height of jejunum villus in LB group was significantly higher than that in LB and LC groups at 49 days (P<0.05). 80-day SC Sanhuang chicken group had significantly higher jejunal crypt depth than 49-day SC group (P<0.05). Compared with the LB group, the Firmicutes and Ruminococcus of Sanhuang chickens in the LA group were significantly up-regulated in the cage or free-range mode (P<0.05). The relative abundance of Bacteroides in LB group was significantly lower than that in caged mode (P<0.05). The experiment indicates that the addition of Clostridium butyricum and compound probiotics to the diet under different feeding modes has no significant effect on the growth performance of Guangxi Sanhuang chickens. In cage or free-range mode, the addition of compound probiotics can promote the morphological development of the jejunum, and the addition of Clostridium butyricum was helpful to increase the relative abundance of beneficial bacteria and the Sanhuang chickens in caged mode were better than those in free-range mode in terms of growth performance, gut morphology and relative abundance of beneficial bacteria. [ABSTRACT FROM AUTHOR]

Objective: To explore and analyze the incidence rate, influencing factors and impact on prognosis of pulmonary hypertension (PH) in patients with Philadelphia chromosome negative myeloproliferative neoplasms (Ph - MPNs)., Methods: The clinical data of 271 patients with Ph - MPNs were retrospectively analyzed, and different disease subtypes were classified. Patients with different disease types were further divided into PH + and PH - groups according to whether HP occurred. Statistical methods were used to analyze the incidence rate, risk factors, and impact on prognosis of PH in Ph - MPNs patients., Results: The overall incidence rate of PH among 271 patients was 26.9%, and according to the classification of disease subtypes, it was found that the incidence rate of PH in patients with primary myelofibrosis (PMF) was significantly higher than those of patients with polycythemia vera and essential thrombocythemia (both P <0.05). Multivariate regression analysis showed that advanced age, long disease course, JAK2 positive and increased hematocrit, lactate dehydrogenase, monocyte count, and uric acid level were independent risk factors for PH in Ph - MPNs patients ( OR >1, P <0.05), and there were some differences in the independent risk factors between different disease subtypes. Survival analysis results showed that the overall survival (OS) rate of PH + patients was significantly lower than that of PH - patients in other types except for PMF (all P <0.05)., Conclusion: The incidence rate of PH in Ph - MPNs patients is high, and its risk factors are diverse. The OS rate of Ph - MPNs patients with PH is low. Therefore, we should be highly alert to the occurrence of PH in Ph - MPNs patients clinically.

Objective: To analyze the clinical characteristics and occurrence of thrombotic/bleeding events of patients with myeloproliferative neoplasm (MPN), and explore the main influencing factors, and create a risk prediction., Methods: The clinical data of 126 MPN patients with BCR-ABL fusion gene negative in the Department of Hematology of Gansu Provincial Hospital from January 2016 to September 2021 were collected, and their clinical characteristics, occurrence of thrombotic/bleeding events and main influencing factors were analyzed and summarized retrospectively. Then, a risk prediction model for thrombotic/bleeding events in MPN patients was constructed., Results: Among 126 MPN patients, 50 patients (39.7%) had experienced thrombotic/bleeding events, including 44 patients (34.9%) with thrombotic events and 6 patients (4.8%) with bleeding events. Among thrombotic diseases, cerebral thrombosis was the most common (23/44, 52.3%), followed by 9 cases of limb artery thrombosis mainly characterized by finger and toe tip artery ischemia, occlusion and gangrene (9/44, 20.5%). Bleeding events included intracerebral hemorrhage and gastrointestinal hemorrhage. Univariate analysis showed that hypertension, hyperhomocysteinemia, white blood cell (WBC) ≥10×10 9 /L, hematocrit (HCT) ≥49%, platelet (PLT) ≥600×10 9 /L and JAK2V617F gene mutation were risk factors for thrombotic/bleeding events in MPN patients, while CALR gene mutation was a protective factor. Multivariate analysis showed that hypertension and PLT≥600×10 9 /L were independent risk factors for thrombotic/bleeding events in MPN patients. The goodness of fit of the constructed risk prediction model was 0.872, and the area under the ROC curve was 0.838. The model was validated with clinical data, the sensitivity, specificity and accuracy was 78.85%, 87.83% and 84.13%, respectively ., Conclusion: The risk of thrombotic/bleeding events in MPN patients with high WBC count, hypertension and hyperhomocysteinemia is higher. Controlling hypertension and hyperhomocysteinemia and reducing WBC and PLT counts are helpful to prevent thrombotic/bleeding events and improve the life quality of patients.

Objective: To investigate the clinical characteristics and prognosis of primary bone marrow lymphoma., Methods: The clinical data of 6 patients with primary bone marrow lymphoma admitted to Gansu Provincial People's Hospital from February 2011 to March 2023 were collected, and their clinical characteristics and prognosis were retrospectively analyzed and summarized., Results: The median age of 6 patients was 61(52-74) years old. There were 2 males and 4 females. All patients had fever and abnormal blood routine examination. Physical examination and imaging examination showed no lymphadenopathy, no extranodal lesions in lung, gastrointestinal, liver and spleen, skin, etc. After strict exclusion of systemic lymphoma involvement in the bone marrow, the diagnosis was confirmed by bone marrow examination, 5 cases were primary myeloid diffuse large B-cell lymphoma and 1 case was primary myeloid peripheral T-cell lymphoma (NOS). 1 case abandoned treatment, 5 cases received CHOP-like or combined R regimen， including 1 case of autologous stem cell transplantation. 4 cases died and 2 case survived. The median OS was 5.5 (1-36) months., Conclusion: The prognosis of primary marrow lymphoma is poor, and bone marrow-related examination is an important means of diagnosis. Diffuse large B-cell lymphoma is the most common histomorphologic and immune subtype, and autologous hematopoietic stem cell transplantation may improve the prognosis.

Objective: To analyze the clinical characteristics and prognosis of patients with CD5 + diffuse large B-cell lymphoma (DLBCL)., Methods: The clinical data of 161 newly treated DLBCL patients in Gansu Provincial Hospital from January 2013 to January 2020 were retrospectively analyzed. According to CD5 expression, the patients were divided into CD5 + group and CD5 - group. The clinical characteristics and prognosis of the two groups were statistically analyzed., Results: The median age of patients in CD5 + group was 62 years, which was higher than 56 years in CD5 - group ( P =0.048). The proportion of women in CD5 + group was 62.96%, which was significantly higher than 41.79% in CD5 - group ( P =0.043). The proportion of patients with IPI score > 2 in CD5 + group was 62.96%, which was higher than 40.30% in CD5 - group ( P =0.031). Survival analysis showed that the median overall survival and progression-free survival time of patients in CD5 + group were 27(3-77) and 31(3-76) months, respectively, which were both shorter than 30(5-84) and 32.5(4-83) months in CD5 - group ( P =0.047, P =0.026). Univariate analysis showed that advanced age, positive CD5 expression, triple or double hit at initial diagnosis, high IPI score and no use of rituximab during chemotherapy were risk factors for the prognosis of DLBCL patients. Further Cox multivariate regression analysis showed that these factors were also independent risk factors except for advanced age., Conclusion: CD5 + DLBCL patients have a worse prognosis than CD5 - DLBCL patients. Such patients are more common in females, with advanced age and high IPI score, which is a special subtype of DLBCL.

Objective: To compare the clinical features and prognosis between newly diagnosed diffuse large B-cell lymphoma (DLBCL) patients with and without hemophagocytic syndrome (HPS)., Methods: The clinical data of 45 DLBCL patients in Gansu Provincial Hospital from January 2012 to December 2021 were retrospectively analyzed. The patients were divided into HPS group (15 cases) and non-HPS group (30 cases). The clinical features and prognosis of the two groups were compared, and survival analysis was performed using Kaplan-Meier method., Results: Patients with HSP were mostly characterized by fever, cytopenia and splenomegaly. The levels of ferritin and soluble CD25 increased in all patients. The level of fibrinogen decreased in 66.67% patients, while triglyceride increased in 53.33% patients, and bone marrow hemophagocytosis occurred in 80.00% patients. Compared with non-HSP group, the proportions of patients with advanced stage (Ann Arbor stage III/IV) and lactate dehydrogenase (LDH) ≥240 U/L were higher in HSP group (both P < 0.05). The median survival time of HSP group was 8.0 months, which was significantly shorter than 45.5 months of non-HSP group ( P < 0.001)., Conclusion: The DLBCL patients with HPS have later Ann Arbor stage, higher LDH and shorter overall survival time compared with patients without HPS.

Objective: To analyze the prognostic nutritional index (PNI), controlling nutritional status (CONUT) and fibrinogen/albumin ratio (FAR) levels in elderly patients with multiple myeloma (MM) and their prognostic impact., Methods: The clinical data of 74 elderly MM patients diagnosed in Gansu Provincial Hospital from January 2020 to July 2022 were retrospectively analyzed. The optimal cut-off values for PNI, CONUT score and FAR were obtained by receiver operating characteristic (ROC) curve, which were used for grouping patients. The correlation of above three indexes with clinical parameters such as sex, serum calcium (Ca), β 2 -microglobulin (β 2 -MG), serum creatinine (Cr) in elderly MM patients were analyzed. The survival rates of patients with different levels of each index were compared. Univariate and multivariate analysis of the impact of clinical indicators on the prognosis of patients were performed., Results: The optimal cut-off values for PNI, CONUT score and FAR were 39.775, 3.5 and 0.175, respectively, according to which the patients were divided into high and low group. Statistical analysis showed that there were significant differences in albumin level among different groups (all P < 0.05). In addition, there was a significant difference in hemoglobin between high-PNI group and low-PNI group ( P < 0.05), while in sex distribution between high-FAR and low-FAR group ( P < 0.05). The survival rate of elderly MM patients with increased PNI, decreased CONUT score and FAR was higher (all P < 0.05). Univariate and multivariate analysis showed that β 2 -MG, Cr, PNI, CONUT score and FAR were independent prognostic factors for elderly MM patients., Conclusion: PNI, CONUT score and FAR are related to some clinical indicators of elderly MM patients, and have an impact on the prognosis.

Objective: To investigate the clinical characteristics, diagnosis, and treatment of one patient with TAFRO syndrome, and to strengthen the understanding of this rare type., Methods: The clinical manifestations, diagnosis and treatment process, and prognosis of the patient admitted in Gansu Provincial People's Hospital were retrospectively analyzed., Results: Combined with laboratory tests, bone marrow examination, imaging, pathology, etc, the patient was diagnosed with TAFRO syndrome. After three cycles of treatment with pomalidomide (2-3 mg/d, d1-21), cyclophosphamide (300 mg/m 2 , 0.54 g once a week) and dexamethasone (20 mg/d, two days a week), platelet count, serum creatinine and procalcitonin returned to normal, the systemic edema disappeared, and the patient's condition was alleviated. The therapeutic effect was good., Conclusion: TAFRO syndrome is rare, involves multiple systems, progresses rapidly, and has a worse prognosis. The choice of the "Pomalidomide+cyclophosphamide+dexamethasone" regimen is help to improve the survival prognosis of patient with TAFRO syndrome.

Objective: To investigate the risk factors and prognosis of cardiovascular damage in hypereosinophilia (HE)., Methods: The clinical data of 62 patients with HE in Gansu Provincial Hospital from January 2015 to December 2020 were retrospectively analyzed, including clinical characteristics and laboratory indicators, and the influencing factors of survival and prognosis were also analyzed., Results: In this study, there were 34 males and 28 females, with a median age of 53.5 (20-79) years, 35 patients without cardiovascular damage, 27 patients with cardiovascular damage, including 22 patients with abnormal electrocardiogram (ECG) (81.5%), 18 patients with abnormal echocardiography (ECHO) (66.7%), 9 patients with single ECG abnormality, 5 patients with single ECHO abnormality, and other 13 patients with multiple abnormalities. In cardiovascular damage group, peripheral white blood cell count, absolute value of eosinophils, troponin T (TNT), N-terminal pro-B-type natriuretic peptide (NT-proBNP), interleukin (IL)-4 and IL-5 levels at initial diagnosis were significantly higher than those in the non-cardiovascular damage group ( P <0.01), while hemoglobin, IL-2 and interferon-γ levels were significantly lower ( P <0.01). There were no significant differences in age, sex, course of disease, etiological classification, platelet count, serum creatine kinase, serum creatine kinase isoenzyme and lactate dehydrogenase between the two groups ( P >0.05). The 5-year overal survival rate of patients with cardiovascular damage was 88.9%, and that of patients without cardiovascular damage was 100%, the difference was statistically significant ( P =0.012). The 5-year event-free survival (EFS) rate of patients with cardiovascular damage was 59.3%, and the median time was 37 (21-52) months, while that of patients without cardiovascular damage was 80%, and the median time was 63 (51-74) months ( P =0.002). Age (>60 years old), course of disease (>24 months), NT-proBNP (>3 000 pg/ml), TNT (>100 ng/L), elevated IL-4 and IL-5 were associated with EFS shortening in patients with cardiovascular damage, which were independent risk factors for EFS., Conclusion: The EFS rate in HE patients without cardiovascular damage is significantly higher than patients with cardiovascular damage. Age, course of disease, NT-proBNP, TNT, IL-4 and IL-5 are independent risk factors affecting EFS of patients with cardiovascular damage.

Objective: To analyze the clinical features and prognosis of patients with Castleman's disease (CD) and improve the diagnosis and treatment of CD., Methods: Clinical data of patients diagnosed with CD by pathological biopsy in Gansu Provincial Hospital from January 2009 to November 2020 were retrospectively analyzed. According to clinical classification, the patients were divided into two groups: UCD (unicentric CD) group ( n =20) and MCD (multicentric CD) group ( n =9). The clinical manifestations, laboratory examination, treatment regimens, pathological examination and follow-up data were statistically analyzed., Results: There were no significant differences in average age and gender ratio between UCD group and MCD group. In UCD patients, 80.0% were hyaline vascular type, and 20.0% were plasma cell type. In MCD patients, 33.3% were hyaline vascular type, 55.6% were plasma cell type, and 11.1% were mixed type. There was significant difference in pathological classification between the two groups (P=0.039). The UCD patients usually presented asymptomatic single lymph node enlargement with mild clinical symptoms, while the MCD patients were characterized by multiple superficial and deep lymph node enlargement throughout the body. The incidences of asthenia, splenomegaly, serous effusion in MCD group were higher than those in UCD group ( P <0.05). Meanwhile, the incidences of anemia, hypoproteinemia, increased ESR, elevated serum globulin and elevated β 2 -microglobulin were significantly higher than those in UCD group too ( P <0.05). There was no significant difference in the incidences of abnormal WBC, PLT and elevated LDH between the two groups ( P >0.05). Among 20 patients with UCD, 13 cases reached complete remission (CR), 1 case achieved partial remission (PR). Among 9 patients with MCD, 3 cases received CR and 4 cases received PR., Conclusion: Patients with CD requires pathological examination for diagnosis. Patients with UCD show mild clinical symptoms, good surgical treatment effect and good prognosis. Patients with MCD have diversified clinical manifestations and relatively poor prognosis, and these patients require comprehensive treatment.

Objective: To investigate the clinical efficacy and safety of bendamustine in the conditioning regimen for autologous stem cell transplantation in patients with lymphoma., Methods: The clinical data of 35 patients with lymphoma, including 5 patients of Hodgkin lymphoma and 30 patients of non-Hodgkin lymphoma, who underwent autologous stem cell transplantation after pretreatment with BeEAM regimen from January 2020 to June 2022 in Gansu Provincial Hospital were retrospectively analyzed. Hematopoietic reconstitution, disease outcome after transplantation and the side effects were analyzed., Results: All 35 patients achieved hematopoietic reconstitution after AHSCT，the median time to neutrophil engraftment was 11 (8-15) days, and the median time to platelet engraftment was 12 (9-17) days. Among the 35 patients, 4 patients died at the end of follow-up, including 3 patients died of lymphoma recurrence or progression and 1 patient died of cerebral hemorrhage. Among 34 patients, 30 had no disease progression at the end of follow-up. The OS rates of patients at 12 and 24 months after transplantation were 90.97% and 90.97%, respectively. The 12 and 24 months PFS rates were 89.64% and 84.92%, respectively. Thiry-five patients underwent grade 3-4 bone marrow suppression. The non-hematological toxicity of BeEAM pretreatment regimen mainly included nausea, vomiting, diarrhea, and oral mucositis, 35 patients experienced nausea and vomiting, but only 4 patients had grade 3-4 nausea and vomiting. Eight patients experienced Grade 1-2 diarrhea. Oral mucositis occurred in 12 patients, including 1 patient of grade 3 oral mucositis. One patient with grade 3 oral mucositis also had grade 3-4 hypokalemia and hypon atremia. 8.6% of patients experienced Grade 1-2 abnormal liver and kidney function. An addition, infectious fever occurred in 18 patients during neutropenia. All patients improved after symptomatic treatment, and there were no transplant-related death., Conclusion: Bendamustine as a pretreatment regimen for autologous stem cell transplantation in lymphoma is effective, and the side effects are tolerable.