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Abstract Key message By monitoring a field experiment over nine years, we investigated the impacts of the two main pathogen species Erysiphe quercicola S. Takam. and U. Braun and Erysiphe alphitoides (Griffon and Maubl.) U. Braun and S. Takam causing powdery mildew on a young cohort of pedunculate oak (Quercus robur L.), both from a demographic and genetic point of view using SNP markers. We show that survival rate is affected by mean disease severity. But while the growth-related tolerance to infection of the oak individual seems to be more determinant than resistance against infection, no equalizing effect of the disease could be detected. Context Studies on the effects of pathogens on the survival and population dynamics of forest trees are scarce. Yet a better understanding of these interactions could prove strategic in the challenging context of climate change. Aims Our general objective was to characterize the demographic and genetic impact of the two main pathogen species Erysiphe quercicola S. Takam. and U. Braun and Erysiphe alphitoides (Griffon and Maubl.) U. Braun and S. Takam causing powdery mildew in the early stages of a Quercus robur L. population. Methods An ad hoc field design with two disease exposures, natural and protected, was surveyed over nine years. This enabled a detailed phenotypic monitoring of 1733 emerging individuals from 15 progenies, and the genotyping of 68% of them. Results The pathogen induced high levels of seedling mortality several years after sowing, associated with reduced growth and capacity to overwinter. Fast-growing families showed the highest survival rate under both natural and protected disease exposure. Contrary to a possible trade-off hypothesis between growth and defense, family height potential was not negatively related to disease resistance across the studied oak mother trees. While supporting a deleterious effect of very low individual heterozygosity on the probability of survival, average genomic diversity was not significantly affected by mortality associated with powdery mildew. Our study also points to a few candidate genes for several fitness-related traits. Conclusion Overall, our results suggest that in oak natural populations, infection levels (related to resistance sensu stricto) may be less determinant than growth-related tolerance to infection for the fate of seedlings. However, an equalizing effect of powdery mildew on relative oak genotype performances cannot be excluded at later stages.

Drawing on signaling theory, the current experimental study (N = 593) investigated the influence of top employer awards on the connections between the full spectrum of narcissistic personality features (i.e., agentic, antagonistic, neurotic, and communal) and perceived company prestige. The results of our experiment show that employer awards enhance prestige perceptions and that all narcissism facets are positively related to perceptions of prestige. However, the positive associations between narcissistic personality features and company prestige were less pronounced in the award condition than in the no-award condition. Our study contributes to the recruitment and employer branding literature by demonstrating that, while employer awards generally enhance an organization’s prestige, they may not do so for individuals with particular personality features.

Major depressive disorder (MDD) is a complex mental health condition with a multifaceted and incompletely elucidated pathophysiology. MicroRNAs (miRNAs) have emerged as potential biomarkers due to their role in gene regulation and the observed dysregulation in MDD. The aim of this study is to detect the presence of specific molecular diagnostic biomarkers in major depressive disorder. This cross-sectional study analyzed plasma miRNA expression in ten MDD patients and eight healthy controls using real-time PCR. Differentially expressed miRNAs were identified using independent t-tests, and their diagnostic potential was assessed with ROC curve analysis. Fifteen miRNAs exhibited significant dysregulation in MDD patients. Notably, hsa-miR-29c-3p, hsa-miR-376a-3p, hsa-miR-532-5p, and hsa-miR-339-5p showed excellent discriminatory power (AUC > 0.8). This study identifies differentially expressed plasma miRNAs in MDD, suggesting their potential for improved diagnosis and personalized treatment. However, further validation in larger cohorts and investigation into their functional roles are warranted.

We investigated whether the associations that self-concept clarity had with indicators of psychological distress were mediated by the forms of escapism referred to as self-suppression (engaging in activities in an effort to avoid unpleasant mental processes) and self-expansion (engaging in activities in order to experience growth). The indirect associations that self-concept clarity had with psychological distress through self-suppression and self-expansion were examined in college students (Study 1; N = 270), Cosplayers (Study 2; N = 45), and Furries (Study 3; N = 191). Across these three studies, self-concept clarity had very large associations with psychological distress and negative associations with self-suppression that ranged from small to large in magnitude. Further, engaging in activities for the purpose of self-suppression mediated the associations that self-concept clarity had with various indicators of psychological distress with the most consistent associations emerging for depression. In contrast, self-concept clarity did not have consistent associations with the motivation to engage in activities for the purpose of self-expansion nor did self-expansion consistently mediate the associations that self-concept clarity had with the indicators of psychological distress. These findings indicate that engaging in activities for the purpose of self-suppression may play an important role in the link between low self-concept clarity and psychological distress.

Respect has been shown to be an important aspect of romantic relationships. The current research explored whether the associations that status-based respect and inclusion-based respect had with romantic commitment would be mediated by satisfaction, investment, and the perceived quality of alternative romantic partners. Study 1 examined these associations with an undergraduate sample (N = 200), whereas Study 2 replicated the results of Study 1 using a community sample (N = 264). Study 3 (N = 187) extended these results by focusing on a specific situation in which participants felt either disrespected or respected by their romantic partners. Across these studies, status-based respect and inclusion-based respect were associated with romantic commitment. For example, status-based respect and inclusion-based respect had associations with commitment that were mediated by satisfaction in each study. The findings of the current studies indicate that people who experience disrespect in their relationships tend to be less satisfied, which, in turn, may lead to them being less committed to maintaining their relationships. In our discussion, we explore how status-based respect and inclusion-based respect can offer insights into understanding commitment to romantic relationships.

The dataset gathers available regulations of human activities and protection levels of Marine Protected Areas (MPAs) of the European Union (EU). The MPA list and polygons were extracted from the MPA database of the European Environment Agency (EEA) and completed with available zoning systems (all were filtered for their marine area reported under the Marine Strategy Framework Directive). Fully-overlapping MPAs were merged. In the resulting dataset, MPA features are provided (gathered from EEA, WDPA, ProtectedSeas), including the year of designation, designation types (e.g., national, Natura 2000) and subtypes (e.g., reserves, national parks), database identifiers (WDPA, Natura 2000, OSPAR, etc.), IUCN categories, and main protection focus. We provide summarized data on maritime activities that overlap with MPA polygons from two types of datasets: activities-focused datasets (national marine spatial plans, and additional European and regional databases, like EMODnet) and MPA-focused datasets gathering data from management plans (ProtectedSeas, expert-based assessments about OSPAR and Portuguese MPAs). This dataset therefore compiles data that could be gathered from accessible legal frameworks regarding aquaculture, fisheries, anchoring, infrastructures (including harbors and renewable energy), mining, transport, coastal land-based uses (desalinization, sewage plants) and other non-extractive uses (e.g., recreational), making them readily accessible. Using the MPA Guide classification system, we computed two scenarios of potential impact for each activity, which were used to assess two scenarios of protection levels per MPA. Some MPAs could not be associated with any MPA features, regulations, or protection levels. Finally, we detail the protocol to match information from multiple databases (e.g., with MPA polygons formatted differently) and provide a quality check by comparing this dataset to previous assessments.This dataset was used to analyze MPAs’ protection levels across countries, regions and MPA features (e.g., IUCN categories, designations). It was also used to investigate the sources of information available and the levels of regulations for each maritime activity in EU MPAs. This dataset can therefore be used for further analyses on the use of EU MPAS to regulate activities and to compare with future assessments or with additional data we did not have access to (e.g., gathered at national scale). Such research is crucial to plan and monitor the implementation of the EU 2030 Biodiversity Strategy, targeting 10% of strictly protected MPAs in each sea region.

IntroductionSevere COVID-19 is associated with reduced absolute lymphocyte counts, suggesting that lymphocyte subsets may serve as predictors of clinical outcomes in affected patients. Early identification of patients at risk for severe disease is crucial for optimizing care, accurately informing patients and their families, guiding therapeutic interventions, and improving patient flow in the ED. Given that immunosuppressive drugs significantly impact lymphocyte profiles, we aimed to determine the association between prior use of immunosuppressive drugs, lymphocyte subsets, and COVID-19 severity in our population with a high prevalence of immunosuppression.MethodsIn 2021, suspected COVID-19 patients were included in the ED. Lymphocyte subsets were determined in peripheral blood within 24 hours after presentation and comparative analyses was performed between SARS-CoV-2 negative and positive patients, mild versus severe disease and patients with and without prior immunosuppressive drug use. Mild cases were patients discharged home or admitted to a general ward, severe cases were patients with COVID-19-related mortality or necessitating ICU admission. Logistic regression analysis was performed to assess the association between lymphocyte subsets and COVID-19 severity, and between prior immunosuppressive drug use and COVID-19 severity.ResultsTwenty-five SARS-CoV-2 negative and 77 SARS-CoV-2 positive patients were included, whereof 57 (74%) had mild and 20 (26%) severe COVID-19. No significant differences were observed in the absolute counts of CD3+, CD4+, and CD8+ T-lymphocytes, B-lymphocytes, and NK-cells between SARS-CoV-2 negative and positive patients or between mild and severe cases. The 36 patients with prior use of immunosuppressive drugs had significantly lower CD4+ T-lymphocytes (p

Summary: Background: Currently, there is no licensed treatment for chronic norovirus infections, but the use of intra-duodenally-delivered immunoglobulins is promising; nevertheless, varying results have limited their wide use. Little is known about the relationship between norovirus genetic diversity and treatment efficacy. Methods: We analyzed the norovirus within-host diversity and evolution in a cohort of 20 immunocompromised individuals using next-generation sequencing (NGS) and clone-based sequencing of the capsid (VP1) gene. Representative VP1s were expressed and their glycan receptor binding affinity and antigenicity were evaluated. Findings: The P2 domain, within the VP1, accumulated up to 30-fold more non-synonymous mutations than other genomic regions. Intra-host virus populations in these patients tended to evolve into divergent lineages that were often antigenically distinct. Several of these viruses were widely resistant to binding-blocking antibodies in immunoglobulin preparations. Notably, for one patient, a single amino-acid substitution in the P2 domain resulted in an immune-escape phenotype, and it was likely the main contributor to treatment failure. Furthermore, we found evidence for transmission of late-stage viruses between two immunocompromised individuals. Interpretation: The findings demonstrated that within-host noroviruses in chronic infections tend to evolve into antigenically distinct subpopulations. This antigenic evolution was likely caused by the remaining low immunity levels exerted by immunocompromised individuals, possibly undermining antiviral treatment. Our observations provide insights into norovirus (within-host) evolution and treatment. Funding: Erasmus MC grant mRACE, the European Union's Horizon 2020 research and innovation program under grant agreement No. 874735 (VEO), and the NWO STEVIN award (Koopmans).

Introduction The use and value of artificial intelligence (AI)-driven tools and techniques are under investigation in detecting coronary artery disease (CAD). EchoGo Pro is a patented AI-driven stress echocardiography analysis system produced by Ultromics Ltd. (henceforth Ultromics) to support clinicians in detecting cardiac ischaemia and potential CAD. This manuscript presents the research protocol for a field study to independently evaluate the accuracy, acceptability, implementation barriers, users’ experience and willingness to pay, cost-effectiveness and value of EchoGo Pro.Methods and analysis The ‘Evaluating AI-driven stress echocardiography analysis system’ (EASE) study is a mixed-method evaluation, which will be conducted in five work packages (WPs). In WP1, we will examine the diagnostic accuracy by comparing test reports generated by EchoGo Pro and three manual raters. In WP2, we will focus on interviewing clinicians, innovation/transformation staff, and patients within the National Health Service (NHS), and staff within Ultromics, to assess the acceptability of this technology. In this WP, we will determine convergence and divergence between EchoGo Pro recommendations and cardiologists’ interpretations and will assess what profile of cases is linked with convergence and divergence between EchoGo Pro recommendations and cardiologists’ interpretations and how these link to outcomes. In WP4, we will conduct a quantitative cross-sectional survey of trust in AI tools applied to cardiac care settings among clinicians, healthcare commissioners and the general public. Lastly, in WP5, we will estimate the cost of deploying the EchoGo Pro technology, cost-effectiveness and willingness to pay cardiologists, healthcare commissioners and the general public. The results of this evaluation will support evidence-informed decision-making around the widespread adoption of EchoGo Pro and similar technologies in the NHS and other health systems.Ethics approval and dissemination This research has been approved by the NHS Health Research Authority (IRAS No: 315284) and the London South Bank University Ethics Panel (ETH2223-0164). Alongside journal publications, we will disseminate study methods and findings in conferences, seminars and social media. We will produce additional outputs in appropriate forms, for example, research summaries and policy briefs, for diverse audiences in NHS.

Aim of the Study: The study aimed to develop and evaluate two new hot chocolate recipes (P1 – Red Recipe and P2 – Green Recipe) and compare them with a commercial hot chocolate product (P3 – “La Festa”). The focus was on creating recipes that are nutritionally comparable to natural or dietetic products, with reduced sugar and increased dietary fibre content. Materials and Methods: The study employed sensory evaluation methods, specifically the Mann-Whitney and Wilcoxon Sign Rank tests, to assess appearance, consistency, flavour, taste, and smell of the hot chocolate recipes. Additionally, a comprehensive nutritional analysis was conducted to compare the protein, lipid, carbohydrate, fibre, and caloric content of the recipes against the commercial product. Results: The sensory evaluation revealed that Recipe P1 significantly outperformed both Recipe P2 and the commercial product P3 in all tested attributes. Nutritionally, Recipes P1 and P2 were similar, both showing lower sugar and higher fibre content than typical market products. However, no significant sensory differences were noted between Recipe P2 and the commercial hot chocolate P3. Conclusions: The study concludes that Recipe P1 is superior in sensory qualities with a favourable nutritional profile, making it an appealing option for health-conscious consumers. Recipe P2, while nutritionally akin to P1, shares more sensory similarities with traditional hot chocolate, offering a healthier alternative without sacrificing familiar flavours. This research underscores the feasibility of creating healthier versions of popular beverages without compromising taste and sensory appeal.

Ragweed pollen allergy is the most common seasonal allergy in western Romania. Prolonged exposure to ragweed pollen may induce sensitization to pan-allergens such as calcium-binding proteins (polcalcins) and progression to more severe symptoms. We aimed to detect IgE sensitization to recombinant Amb a 9 and Amb a 10 in a Romanian population, to assess their potential clinical relevance and cross-reactivity, as well as to investigate the relation with clinical symptoms. rAmb a 9 and rAmb a 10 produced in Escherichia coli were used to detect specific IgE in sera from 87 clinically characterized ragweed-allergic patients in ELISA, for basophil activation experiments and rabbit immunization. Rabbit rAmb a 9- and rAmb a 10-specific sera were used to detect possible cross-reactivity with rArt v 5 and reactivity towards ragweed and mugwort pollen extracts. The results showed an IgE reactivity of 25% to rAmb a 9 and 35% to rAmb a 10. rAmb a 10 induced basophil degranulation in three out of four patients tested. Moreover, polcalcin-negative patients reported significantly more skin symptoms, whereas polcalcin-positive patients tended to report more respiratory symptoms. Furthermore, both rabbit antisera showed low reactivity towards extracts and showed high reactivity to rArt v 5, suggesting strong cross-reactivity. Our study indicated that recombinant ragweed polcalcins might be considered for molecular diagnosis.

Abstract Waning antibody responses after COVID-19 vaccination combined with the emergence of the SARS-CoV-2 Omicron lineage led to reduced vaccine effectiveness. As a countermeasure, bivalent mRNA-based booster vaccines encoding the ancestral spike protein in combination with that of Omicron BA.1 or BA.5 were introduced. Since then, different BA.2-descendent lineages have become dominant, such as XBB.1.5, JN.1, or EG.5.1. Here, we report post-hoc analyses of data from the SWITCH-ON study, assessing how different COVID-19 priming regimens affect the immunogenicity of bivalent booster vaccinations and breakthrough infections (NCT05471440). BA.1 and BA.5 bivalent vaccines boosted neutralizing antibodies and T-cells up to 3 months after boost; however, cross-neutralization of XBB.1.5 was poor. Interestingly, different combinations of prime-boost regimens induced divergent responses: participants primed with Ad26.COV2.S developed lower binding antibody levels after bivalent boost while neutralization and T-cell responses were similar to mRNA-based primed participants. In contrast, the breadth of neutralization was higher in mRNA-primed and bivalent BA.5 boosted participants. Combined, our data further support the current use of monovalent vaccines based on circulating strains when vaccinating risk groups, as recently recommended by the WHO. We emphasize the importance of the continuous assessment of immune responses targeting circulating variants to guide future COVID-19 vaccination policies.

(1) Background: The aim of this study was to analyze the impact of pharmacogenetic-guided antidepressant therapy on the 12-month evolution of the intensity of depressive symptoms in patients with recurrent depressive disorder (RDD) in comparison to a control group of depressive subjects who were treated conventionally. (2) Methods: This prospective longitudinal study was conducted between 2019 and 2022, and the patients were evaluated by employing the Hamilton Depression Rating Scale (HAM-D), Hamilton Anxiety Rating Scale (HAM-A) and the Clinical Global Impressions Scale: Severity and Improvement. We followed them up at 1, 3, 6, and 12 months. (3) Results: Of the 76 patients with RDD, 37 were tested genetically (Group A) and 39 were not (Group B). Although the patients from Group A had statistically significantly more severe MDD at baseline than those from Group B (p < 0.001), by adjusting their therapy according to the genetic testing, they had a progressive and more substantial reduction in the severity of RDD symptoms [F = 74.334; η2 = 0.674; p < 0.001], indicating a substantial association with the results provided by the genetic testing (67.4%). (4) Conclusions: In patients with RDD and a poor response to antidepressant therapy, pharmacogenetic testing allows for treatment adjustment, resulting in a constant and superior reduction in the intensity of depression and anxiety symptoms.

Abstract Metallosis with subsequent cardiac involvement is a possible long‐term complication of hip arthroplasty. We report the case of a young female referred to our centre for the suspicion of cardiac amyloidosis presenting with low electrocardiogram voltage, left ventricular hypertrophy, pericardial effusion, and global and longitudinal systolic impairment with apical sparing pattern. Her medical history was remarkable for arthroplasty in the context of congenital hip dysplasia. Two years prior to presentation, she underwent revision surgery for prosthesis malfunction, and tissue metallosis was initially documented. At the current presentation, cobalt metallosis was confirmed, as the circulating cobalt and chromium levels were severely elevated. The accurate diagnosis prompted the removal of the cobalt source with extensive tissue debridement and the use of chelating agents. Reversal of the cardiac abnormalities occurred as the circulating cobalt levels returned to normal.

# Background E-learning plays a key role in bridging competency gaps among the public health workforce to prevent, detect, and respond to public health emergencies. However, its implementation remains a challenge in resource-limited settings. In this study, we assessed the contextual fit and feasibility of a multi-communication online training (MOT) for its effective implementation. # Methods A mixed-methods study was conducted among public health workers in 16 West African countries between Aug 10, 2023, and Oct 10, 2023. Data were collected through an online survey and in-depth interviews. Through a One Health forum and subregionally coordinated efforts, participants were sampled for the survey and interviews using virtual snowball and voluntary response techniques respectively. Contextual fit was measured with MOT preference and acceptability, while feasibility was measured with MOT willingness to use and workplace ICT availability using a novel tool. Statistical weighting was applied, and interviews were thematically analysed. Quantitative data were analysed using SAS version 9.4 with inverse probability weights and post-stratification weights at the country level. Descriptive statistics were summarized as unweighted and weighted frequencies and weighted proportions. Rao-Scott Chi-square test and Fisher’s exact test were used to identify factors with prognostic potential that were subjected to bivariate and multivariate analyses with a binary logit regression model to assess associations. Qualitative data were managed using Microsoft Excel and MAXQDA 10. # Results A total of 231 survey responses were collected and seven interviews conducted. MOT was found to be of “somewhat” contextual fit with population estimates of (preference: 29.61%, CI= 23.96-35.27; acceptability: 95.99%, CI= 93.79-98.18), and “strong” feasibility (willingness to use: 95.56%, CI= 93.78-97.35; workplace ICT availability: 82.09%, CI= 77.50-86.68). Work area was found as a major predictor of MOT contextual fit and feasibility, where the probabilities of MOT preference, acceptability, willingness to use, and workplace ICT availability were 42% lower (CI= 0.58-0.59), 99% lower (CI= 0.01-0.14), 95% lower (CI= 0.01-0.40), and 2.57 (CI= 1.22-5.40) times higher respectively for public health workers in rural areas compared to those in urban areas. Triangulating the quantitative and qualitative data showed issues around training resources accessibility, training characteristics, and training environment as factors that contributed to the contextual fit and feasibility ratings. # Conclusions This study findings provided evidence for the contextual fit and feasibility of MOT with geographic disparities, including associated factors that could inform prioritization, adaptation, and scale up of e-learning interventions through the equity lens towards improving the public health workforce capacity for global health security.

Abstract Intensive resource extraction activity in the oil sands of Canada alters the quantity, structure and distribution of native ecosystems, which in turn creates substantive challenges for conservation, land management and habitat reclamation. Progressive reclamation occurs on active mine sites in the Athabasca oil sands region (AOSR) of Canada, along with concurrent assessments of reclamation effectiveness. Yet, little is known about the ability of reclaimed habitats to mitigate for the short‐ and long‐term impacts associated with open‐pit mines and provide functional habitat for wildlife. We used a robust quasi‐experiment that combined camera trap data from an observational study with an innovative occupancy model to assess the effectiveness of upland reclamation to provide habitat for wildlife in the AOSR. The dynamic occupancy models were applied to 15 years of camera trap data to assess wildlife usage patterns of nine species of wildlife over seven types of habitats. The habitats sampled ranged from mining‐disturbed habitats reclaimed to upland forest ecosites common in the region, habitats disturbed by natural (fire) and human disturbances (clear‐cut logging), with comparisons to mostly intact mature forests. Our results indicate that the nine species of wildlife assessed used habitats in a manner consistent with expectations: some preferred disturbance‐dominated habitats while others used mature forest to a higher degree. We demonstrate that the application of dynamic occupancy models to camera trap data reliably discerned these trends, providing the means to predict wildlife usage patterns in an ever‐changing landscape, including one containing bitumen extraction as a contributor to landscape‐level modifications. Practical implication: Our work illustrates how continued monitoring of wildlife using camera traps contributes to assessments of reclamation effectiveness with respect to wildlife occurrence, distribution and usage patterns in anthropogenically and naturally disturbed landscapes. Evaluating the effectiveness of reclamation is especially important given the expected increase in habitat reclamation on active oil sands mines over the next several decades, coupled with the need to ensure that disturbed habitats regain ecological function able to sustain wildlife in the future.

Mangalia area harbors in the western Black Sea a distinctive marine environment thriving under specific hydrochemical conditions, largely influenced by a significant number of sulphurous springs occurring in shallow marine waters. These springs led to the designation of the area as part of the Natura 2000 Marine Protected Area (MPA) network at European level (Underwater Sulphurous Springs from Mangalia - ROSAC0094), as unique hydro-geomorphological features in the region. In 2021 and 2023, two research cruises led by GeoEcoMar investigated underwater sulphurous springs primarily located offshore of Mangalia (Constanţa County, Romania). The study area, located between 17-29 meters water depth and 1.8-3 km offshore, encompasses two marine protected areas: the Underwater Sulphurous Springs from Mangalia (ROSAC0094) and Cape Aurora (ROSCI0281). The research combined geophysical and geochemical techniques and sediment sampling. Considering the susceptibility of these natural systems to human activities such as fishing and dredging, as well as the impact of ecological and climate changes, this paper offers significant insights contributing to the development of effective conservation and management strategies for these environments. The surveys were conducted for benthic habitats mapping, with the objective of improving our understanding of these ecosystems’ distribution, composition and dynamics. As these sulphurous waters are rich in methane, a powerful greenhouse gas, our results also contribute to the inventory of greenhouse gas sources. The results presented in this paper provide valuable new insights into this specific environment, contributing to the understanding of its complex functioning and evolution.

Quercetin is a natural flavonoid renowned for its potent antioxidant, anti-inflammatory, anti-diabetic, and antibacterial properties, making it a highly promising candidate for the treatment of various medical conditions. Our current study investigates the influence of quercetin on energy metabolism, fatty acid composition, oxidative stress gene expression, and sirtuin expression in early- and late-stage passages of stem cells derived from human exfoliated deciduous teeth (SHEDs). Mitochondrial respiration was analyzed by measuring oxygen consumption following a 24 h quercetin treatment, while fatty acid profiles were examined using gas chromatography–mass spectrometry (GC-MS). Additionally, quantitative PCR (qPCR) was used to assess the expression of oxidative stress genes and sirtuins. In younger SHEDs, quercetin enhances metabolic activity and mitochondrial respiration, although higher doses may decrease mitochondrial activity. Conversely, in older, senescent SHEDs, quercetin supports mitochondrial function at lower concentrations but appears to inhibit respiration at higher doses. These results suggest that quercetin may hold therapeutic potential for maintaining SHED viability and function, especially at lower doses in older cells. Further research is essential to fully elucidate a dose-dependent effect of quercetin and optimize its applications in regenerative medicine.

Cultural traditions in Israel’s Bedouin-Arab community encourage and permit men to take up to four wives, a practice supported by Islamic teachings. Despite legal prohibitions against polygyny in Israel, such marriages remain common and have profound effects on women, children, and broader society. This study explores how traditional notions of masculinity and positive attitudes toward polygyny influence young Bedouin men’s reactions to a hypothetical scenario in which their wife refuses to accept a polygynous marriage as a threat to men’s ego and pride. The sample consisted of 459 young, unmarried Israeli Bedouin-Arab men, aged 16 to 25, a demographic frequently under family pressure to marry, making the issue of marriage highly relevant. We hypothesized that traditional masculinity and favorable attitudes toward polygyny would be positively associated with heightened perceptions of ego and pride threats in response to a wife’s refusal. Participants were randomly assigned to imagine either their wife refusing a polygynous arrangement (n = 228) or accepting a polygynous arrangement (n = 231) and then reporting their anticipated emotional responses. The results showed that traditional masculinity was linked to positive attitudes toward polygyny, and both were significantly associated with increased perceptions of ego and pride threats in the refusal scenario but not in the acceptance scenario. The findings suggest that men with strong masculine identities and favorable views of polygyny are especially vulnerable to feeling threatened by a wife’s refusal. This supports social construction theory and underscores the role of cultural norms in shaping emotional responses. The discussion highlights the psychological impacts of polygyny in patriarchal societies and calls for culturally sensitive interventions that challenge traditional norms while fostering psychological resilience and gender equality.

Background: The dopaminergic theory, the oldest and most comprehensively analyzed neurotransmitter theory of schizophrenia, remains a focal point of research. Methods: This systematic review examines the association between combinations of 14 dopaminergic genes and the risk of schizophrenia. The selected genes include dopamine receptors (DRD1–5), metabolizing enzymes (COMT, MAOA, MAOB, DBH), synthesizing enzymes (TH, DDC), and dopamine transporters (DAT, VMAT1, and VMAT2). Results: Recurring functional patterns show combinations with either hyperdopaminergic effects in limbic and striatal regions or high striatal and low prefrontal dopamine levels. The protective statuses of certain alleles or genotypes are often maintained in epistatic effects; however, exceptions exist. This complexity could explain the inconsistent results in previous genetic studies. Investigating individual alleles may be insufficient due to the heterozygous advantage observed in some studies. Conclusions: Schizophrenia may not be a monolithic disease, but rather a sum of different phenotypes which respond uniquely to different treatment and prevention approaches.

Thorium is a weak radioactive element, but the control of its concentration in natural aqueous systems is of great interest for health, because it is a toxic heavy metal. The present paper presents the recovery of thorium from diluted synthetic aqueous systems by nanofiltration. The membranes used for the nanofiltration of systems containing thorium species are composites containing 4′-Aminobenzo-15-crown-5 ether (ABCE) and sulfonated poly–etherether–ketone (sPEEK). The composite membranes (ABCE–sPEEK) were characterized by scanning electron microscopy (SEM), energy-dispersive X–Ray spectroscopy (EDAX), thermal analysis (TG and DSC), and from the perspective of thorium removal performance. To determine the process performance, the variables were the following: the nature of the composite membrane, the concentration of thorium in the aqueous systems, the rotation speed of the stirrer, and the pressure and the pH of the thorium aqueous system. When using pure water, a permeate flux value of 12 L·m−2 h−1 was obtained for the sPEEK membrane, and a permeate flux value of up to 15 L·m−2 h−1 was obtained for the ABCE–sPEEK composite membrane. The use of mechanical stirring, with a propeller stirrer, lead to an increase in the permeate flux value of pure water by about 20% for each of the studied membranes. Depending on the concentration of thorium and the pH of the feed solution, retentions between 84.9% and 98.4% were obtained. An important observation was the retention jump at pH 2 for the ABCE–sPEEK composite membrane. In the paper, a thorium ion retention mechanism is proposed for the sPEEK membrane and the ABCE–sPEEK composite membrane.

Aristolochia sp. plants are used in traditional medicine because of their immunostimulatory and anticarcinogenic properties, despite their content of aristolochic acids (AAs), carcinogenic and nephrotoxic agents. Therefore, ethanolic extracts of Aristolochia clematitis leaves, a specie growing in Western Romania, were obtained to study antioxidant and cytotoxic effects. The antioxidant capacity of the extract was evaluated by five in vitro chemical-based assays, proving that ABTS assay was a better method for this type of evaluation showing an IC50 of 160.89 ± 0.21 µg/mL. Furthermore, the cytotoxic effects of the extract were established by an IC50 of 216 µg/mL for 24 h by MTT assay, followed by a cell-based assay on Caco-2 cells by the ABTS method. The antioxidant effects of the A. clematitis extract demonstrate potential therapeutic applications in complementary medicine.

This study aimed to examine the role of travel-related motivations and preferences for activities (such as learning about local culture, relaxation and entertainment, status and social recognition, escape and curiosity, and experience) in the relationship between narcissistic personality traits and the preference for luxury tourism versus cultural tourism. A sample of 1342 Israeli community members was analyzed. The methodology utilized parallel mediation analyses to assess how different forms of narcissism (extraverted, antagonistic, neurotic, and communal) were associated with the desire for luxury tourism over cultural tourism, as mediated by various travel-related motivations. The results indicated that narcissistic personality traits were generally associated with a preference for luxury tourism, primarily through indirect associations via travel motivations such as relaxation and entertainment, status and social recognition, and experience. The conclusion highlights the implications for understanding how specific travel-related motivations influence tourism preferences among individuals with varying levels of narcissistic traits, suggesting that tourism management strategies could benefit from a nuanced approach to personality in marketing.

Orbital angular momentum of light and the associated Laguerre–Gauss modes have garnered increasing interest due to the many applications they promise in the fields of telecommunications and quantum information, as well as charged particle acceleration. Here, we use the numerical simulation of the dynamics of a large number of identical, charged, relativistic, but classical particles, forming a statistical ensemble and seen as test-particles in a time-dependent external electromagnetic field, to get insight into the process of a head-on collision with a pulsed Laguerre–Gauss mode. We have identified two steady-states regimes (low a0 and large a0), where the distribution of the final angular momentum gained by the particles as a function of their initial coordinates is well-behaved and does not change its structure with variations of the reduced amplitude. For small reduced amplitudes a0 of the incoming electromagnetic field, we have also found an analytic approximation of the expression for the angular momentum transferred to the particles after the pulse has left their region. Using both numerical integration and the analytic approximation, we have investigated the effects of the field’s gradient, initial phase of the field, polarization type, and azimuthal order m.

Objective: To describe the adaptations made and to examine interrater reliability and feasibility of administering a telehealth version of the Multiple Sclerosis Functional Composite (tele-MSFC). Design: The Multiple Sclerosis Functional Composite (MSFC) is a commonly used, in-person clinical outcome assessment. It is composed of the timed 25-Foot Walk Test (T25FWT), Nine-Hole Peg Test (NHPT), and Paced Auditory Serial Addition Test (PASAT). The MSFC was adapted for videoconference administration as part of a larger clinical trial. One of the adaptations included administering a timed 12.5-Foot Walk Test (T12.5FWT) for participants who did not have adequate space in their homes for the T25FWT. Participants, examiners, and raters completed surveys online about their satisfaction and experience with tele-MSFC. Setting: Participants underwent the tele-MSFC in their homes using a laptop or smartphone while examiners scored the tele-MSFC in real-time at a remote location. Participants: Community-dwelling adults (n=61) with mild-to-moderate multiple sclerosis (MS) symptoms. Interventions: Not applicable. Main Outcome Measure: Tele-MSFC. Results: Intraclass correlation coefficients (ICC) assessed interrater reliability between the examiner and 2 independent raters who later scored a recording of the tele-MSFC. Interrater reliability was excellent (ICC>0.90) for all tests, including the T12.5FWT. Participants were highly satisfied with tele-MSFC. However, challenges included adequate space for T25FWT, technical difficulties, and safety and privacy considerations of individuals with moderate impairments who were requested to have their caregivers present during testing. Conclusion: The tele-MSFC is reliable and feasible to administer with adaptations for community-dwelling adults with mild to moderate MS symptoms. Further validation of T12.5FWT is needed.

IntroductionSarcoidosis is a multi-system inflammatory disease of unknown origin with heterogeneous clinical manifestations varying from a single organ non-caseating granuloma site to chronic systemic inflammation and fibrosis. Gene expression studies have suggested several genes and pathways implicated in the pathogenesis of sarcoidosis, however, due to differences in study design and variable statistical approaches, results were frequently not reproducible or concordant. Therefore, meta-analysis of sarcoidosis gene-expression datasets is of great importance to robustly establish differentially expressed genes and signalling pathways.MethodsWe performed meta-analysis on 22 published gene-expression studies on sarcoidosis. Datasets were analysed systematically using same statistical cut-offs. Differentially expressed genes were identified by pooling of p-values using Edgington’s method and analysed for pathways using Ingenuity Pathway Analysis software.ResultsA consistent and significant signature of novel and well-known genes was identified, those collectively implicated both type I and type II interferon mediated signalling pathways in sarcoidosis. In silico functional analysis showed consistent downregulation of eukaryotic initiation factor 2 signalling, whereas cytokines like interferons and transcription factor STAT1 were upregulated. Furthermore, we analysed affected tissues to detect differentially expressed genes likely to be involved in granuloma biology. This revealed that matrix metallopeptidase 12 was exclusively upregulated in affected tissues, suggesting a crucial role in disease pathogenesis.DiscussionOur analysis provides a concise gene signature in sarcoidosis and expands our knowledge about the pathogenesis. Our results are of importance to improve current diagnostic approaches and monitoring strategies as well as in the development of targeted therapeutics.

The vortex photon beam induced nuclear reaction is studied. The interaction formalism of nuclei with vortex photons is developed and incorporated into the statistical reaction model to calculate reaction cross-sections. For 138 nuclei of high nuclear astrophysics and structure interest, the cross-sections of γ-ray emission and neutron production from the decay of the giant resonances (GR) populated by vortex photons, e.g., (γvo,γemit) and (γvo,nprod.), are computed. It is shown that for the (γvo,γemit) and (γvo,nprod.) cross-sections, the GR contribution of an individual L is either enhanced or suppressed depending on the parameters of vortex γ-rays, and the contribution from the GR of a specific L can be identified and deduced. To this end, a novel method to exclusively determine the γ-strength function (γSF) for the GR of a specific L is proposed considering the (γvo,γemit) and (γvo,nprod.) measurements, and the feasibility studies demonstrate that the γSF for the giant quadrupole resonance can be extracted. Furthermore, the astrophysical reaction rates of the vortex photon induced reactions in p-process are investigated. It is indicated that photo-nuclear reactions induced by vortex photons will bring new insights in nuclear physics and astrophysics research.

PurposePrevious studies have demonstrated that the majority of patients with an inborn error of immunity (IEI) develop a spike (S)-specific IgG antibody and T-cell response after two doses of the mRNA-1273 COVID-19 vaccine, but little is known about the response to a booster vaccination. We studied the immune responses 8 weeks after booster vaccination with mRNA-based COVID-19 vaccines in 171 IEI patients. Moreover, we evaluated the clinical outcomes in these patients one year after the start of the Dutch COVID-19 vaccination campaign.MethodsThis study was embedded in a large prospective multicenter study investigating the immunogenicity of COVID-19 mRNA-based vaccines in IEI (VACOPID study). Blood samples were taken from 244 participants 8 weeks after booster vaccination. These participants included 171 IEI patients (X-linked agammaglobulinemia (XLA;N=11), combined immunodeficiency (CID;N=4), common variable immunodeficiency (CVID;N=45), isolated or undefined antibody deficiencies (N=108) and phagocyte defects (N=3)) and 73 controls. SARS-CoV-2-specific IgG titers, neutralizing antibodies, and T-cell responses were evaluated. One year after the start of the COVID-19 vaccination program, 334 study participants (239 IEI patients and 95 controls) completed a questionnaire to supplement their clinical data focusing on SARS-CoV-2 infections.ResultsAfter booster vaccination, S-specific IgG titers increased in all COVID-19 naive IEI cohorts and controls, when compared to titers at 6 months after the priming regimen. The fold-increases did not differ between controls and IEI cohorts. SARS-CoV-2-specific T-cell responses also increased equally in all cohorts after booster vaccination compared to 6 months after the priming regimen. Most SARS-CoV-2 infections during the study period occurred in the period when the Omicron variant had become dominant. The clinical course of these infections was mild, although IEI patients experienced more frequent fever and dyspnea compared to controls and their symptoms persisted longer.ConclusionOur study demonstrates that mRNA-based booster vaccination induces robust recall of memory B-cell and T-cell responses in most IEI patients. One-year clinical follow-up demonstrated that SARS-CoV-2 infections in IEI patients were mild. Given our results, we support booster campaigns with newer variant-specific COVID-19 booster vaccines to IEI patients with milder phenotypes.

Tourism is called upon to play the role of facilitator of sustainable development, to lead collaboration with interest groups and local communities to decide jointly on the development of destinations. After the last difficult and challenging years, tourism has shown its resilience and ability to once again represent the percentage of GDP of many of the countries it owned before the pandemic. The WTO launched in 2021 the "Best Tourism Villages" initiative with the aim of transforming tourism into an engine for rural development and well-being and bringing about real change in local communities by creating jobs, supporting local businesses and preserving customs and traditions. The initiative recognizes villages that are an example of a rural tourist destination with cultural and natural assets, that preserve and promote the values, products and lifestyles of communities, and that have a clear commitment to innovation and sustainability. Romania is very well positioned at international level, from the point of view of rural tourism. Six localities have entered the competition "Best Tourism Villages" (2022), dedicated to the recognition of villages that are remarkable examples of rural tourist destinations. These are Biertan, Răşinari and Râu Sadului (Sibiu County), Ciocăneşti (Suceava), Costeşti (Vâlcea), Eibenthal (Mehedinţi). In the work we analysed the criteria that formed the basis for the selection of the three villages in Sibiu County, namely: the cultural and natural resources in the destination, the promotion and conservation of cultural resources, the economic, social and environmental sustainability, the governance and prioritization of tourism, infrastructure and connectivity, health, safety and security, as well as the photos and presentation videos. Răşinari village is the big winner of Romania's second edition of the world competition Best Tourism Villages, being selected by the specialists of the WTO, from over 130 applications, coming from 57 countries.

The present study concerned the connection between the desire for power and romantic commitment in LGBTQ relationships as well as the roles that indicators of relationship functioning (e.g., relationship satisfaction) and perceptions of power played in this association. These associations were examined in community members (N = 113) involved in an LGBTQ romantic relationship. The desire for power had a negative association with romantic commitment that was mediated by perceptions of the relationship and moderated by perceived power. Similar to the results of previous studies concerning heterosexual relationships, these results suggest that issues surrounding the desire for power may be intimately connected with romantic commitment in LGBTQ relationships.

The aim of the study is to perform a comparison between conventional impression versus intraoral and extraoral scanning, plaster models compared to virtual and printed models, in terms of working time and fidelity. Material and method: The study evaluates two groups of practitioners: 30 dentists and 30 dental technicians, regarding registration and reproduction of the prosthetic field. The time required to perform the operations was evaluated in relation to variables that can influence the final results. Conventional impressions were monophasic and biphasic. In the digital flow, an intraoral scanner (Medit, i700) and a laboratory scanner (Medit T310) were used. Also, a printing machine (Elegoo Saturn) was used to print the models. Results and discussions: The average time allocated for conventional impressions is between 8min and 20sec and 25min and 40sec. The scanning time varied between 5 min 30 sec and 12 min 40 sec. Conclusions: The digital impression requires less working time compared to the conventional one, the latter cannot be completely abandoned, at least in terms of mobile prostheses. The fidelity of the prosthodontic field reproduction influences the adaptation of the restorations.

Aim of the study is an ex-vivo time analysis comparing conventional and digital impression methods for prosthodontic restorations. Insights gleaned from ex-vivo experiments hold promise for enhancing existing practices. This primary experimental investigation will prioritize the consideration of both clinician and patient requirements when determining treatment modalities. Materials and Method: The study involved five stages: preparing an artificial arch, measuring time for gingival retraction with cords and paste, assessing time for conventional and digital impressions, and data analysis. A Frasaco maxillary model with 16 teeth was utilized, 14 prepared using cylindrical-conical burs. Gingival retraction cords and paste were applied, and impression times were recorded for both conventional and digital methods. Results and Discussions: Time analyses showed varying durations for gingival retraction techniques, with the paste method significantly faster than retraction cords. Digital impressions exhibited shorter scanning times compared to conventional methods, especially for fewer number of teeth. However, conventional two-step impressions took longer but provided better cervical area fidelity. This study highlighted the efficiency of digital scanning, offering shorter impression times and reduced patient discomfort. Research also emphasized the differences between retraction methods, with varied effects on periodontal tissues and impression quality. Comparison studies indicated benefits and challenges associated with different impression techniques. Conclusion: The findings underscored the need for practitioners to choose impression methods based on time efficiency and patient needs. Optical scanning proved quickest for fewer teeth, while retraction cord and paste methods require careful consideration. While the study didn’t explore gingival tissue effects, it highlighted crucial considerations for impression materials and chemical solutions. These insights offer practitioners guidance in selecting optimal techniques for effective and efficient dental procedures.

The present study explored potential factors that may mediate the relationship between narcissism and conspicuous consumption, as the underlying reasons for this connection remain uncertain. Specifically, we investigated the following variables as potential mediators of the links between narcissistic personality traits and conspicuous consumption: consumer need for uniqueness, materialism, approval seeking, material meaning, consumer sexual signaling, and need for social status. In a sample of 2,944 Israeli community members, our study revealed that extraverted, antagonistic, neurotic, and communal narcissism frequently exhibited positive indirect connections with conspicuous consumption through these mediating factors, with the most robust associations often being observed for antagonistic narcissism. However, a clear departure from this pattern became apparent when the anticipated mediation of the connections between narcissistic personality traits and conspicuous consumption via the need for social status did not unfold as predicted. In contrast, extraverted, antagonistic, and communal narcissism exhibited negative indirect associations with conspicuous consumption through the need for social status. Collectively, these findings indicate both commonalities and distinctions in how narcissistic personality traits are associated with conspicuous consumption via specific mediators. The discussion delves into the significance of these outcomes in shedding light on why conspicuous consumption holds allure for individuals with narcissistic tendencies.

ABSTRACT: Objectives: Elderly become more susceptible to lower respiratory tract infections, resulting in antibiotic prescriptions. Immunoglobulins (Ig) play an important role in host defense and protection against infections. Therefore, we aimed to investigate whether lower Ig levels are a risk factor for antibiotic use in the general elderly population. Methods: After exclusion of current antibiotic users, Cox proportional-hazards regression models were performed to investigate the effect of stable serum IgM, IgG and IgA levels on time to first antibiotic prescription within the Rotterdam Study. Regression models were adjusted for age, sex, body mass index, smoking status and diabetes. We introduced quadratic terms and additionally categorized Igs to explore and quantify potential non-linearity of the association. The restricted cubic splines technique was used to plot the natural log of the hazard across Ig level. Results: In total, 8,639 participants were included (mean age 64 years, 57% female, medium follow-up 3.2 years). No significant association between IgM and time to antibiotic prescription was observed. IgG and IgA levels (in g/L) showed a U-shaped relationship with time to antibiotic prescription (linear IgG HR 0.959, 95% CI 0.930–0.989; quadratic IgG² HR 1.002, 95% CI 1.000–1.003; linear IgA HR 0.949, 95% CI 0.910–0.990; quadratic IgA² HR 1.009, 95% CI 1.004–1.013). Conclusion: Both low and high IgG and IgA levels were associated with a higher incidence of antibiotic prescriptions in stable middle-aged and older individuals. Increased awareness for the potential increased infection risk when persons have low or high Ig levels, even within the reference ranges, is needed.

Abstract Background Inflammation plays a key role in the development of dementia, but its link to early biomarkers, particularly those in plasma or neuroimaging, remains elusive. This study aimed to investigate the association between serum immunoglobulins and biomarkers of dementia. Methods Between 1997 and 2009, serum immunoglobulins (IgA, IgG and IgM) were measured in dementia-free participants of the population-based Rotterdam Study. A random subset of participants had assessment of biomarkers in plasma (total tau (t-tau), neurofilament light chain (NfL), amyloid-β40 (Aβ-40), amyloid-β42 (Aβ-42), while another subset of participants underwent neuroimaging to quantify brain volume, white matter structural integrity and markers of cerebral small vessel disease. Linear regression models were constructed to determine cross-sectional associations between IgA, IgG, IgM and biomarkers of dementia, with adjustment for potential confounders. Multiple testing correction was applied using the false discovery rate. As a sensitivity analysis, we re-ran the models for participants within the reference range of immunoglobulins, excluding those using immunomodulating drugs, and conducted a stratified analysis by APOE-ε4 carriership and sex. Results Of 8,768 participants with serum immunoglobulins, 3,455 participants (65.8 years [interquartile range (IQR): 61.5–72.0], 57.2% female) had plasma biomarkers available and 3,139 participants (57.4 years [IQR: 52.7–60.7], 54.4% female) had neuroimaging data. Overall, no associations between serum immunoglobulins and biomarkers of dementia remained significant after correction for multiple testing. However, several suggestive associations were noted: higher serum IgA levels concurred with lower plasma levels of Aβ-42 (standardized adjusted mean difference: -0.015 [95% confidence interval (CI): -0.029−-0.002], p = 2.8 × 10–2), and a lower total brain volume, mainly driven by less gray matter (-0.027 [-0.046−-0.008], p = 6.0 × 10–3) and more white matter hyperintensities (0.047 [0.016 – 0.077], p = 3.0 × 10–3). In sensitivity analyses, higher IgM was linked to lower t-tau, Aβ-40, and Aβ-42, but also a loss of white matter microstructural integrity. Stratified analyses indicate that these associations potentially differ between carriers and non-carriers of the APOE-ε4 allele and men and women. Conclusions While associations between serum immunoglobulins and early markers of dementia could not be established in this population-based sample, it may be valuable to consider factors such as APOE-ε4 allele carriership and sex in future investigations.

The monumental trees growing in the cities, especially those from the parks, green spaces and alignments, which potentially cause a great risk to people, are required from time to time to be checked about their health conditions. The aim of this article was to assess the health state of the seven monumental trees from Brasov using ultrasonic tomography with an ARBOTOM 2D software (Rinn Tech, Germany). The material under research was the seven monumental trees that grows in Brasov area, respectively: silver fir tree, sweet chestnut tree, beech tree, ginkgo, horsechestnut tree, sycamore tree and plane tree, whose health status was checked using a sound tomograph which allows to assess the inside of the trunk in a non-invasive way. It could be observed that of the seven monumental trees investigated, two of them are in good status of health (Ginkgo tree and sycamore tree), three specimens show visible effect of decay processes of wood (65% horsechestnut tree, 75% sweet chestnut tree and 31% plane tree) and two specimens respectively silver fir tree and beech tree have the cross-section of their trunks with a beginning of rot in a percentage of 65%.

We investigated the roles that suspicious jealousy and reactive jealousy might play in the associations between borderline personality features (BPF) and mate retention behaviors. Study 1 (N = 406) found that BPF had positive indirect associations with benefit-provisioning behaviors and cost-inflicting behaviors through suspicious jealousy but not through reactive jealousy. Study 2 (N = 334 (a dyadic sample of 167 romantic couples)) revealed actor effects such that BPF had positive indirect associations with benefit-provisioning behaviors and cost-inflicting behaviors through suspicious jealousy for both men and women. In addition, the positive association between BPF and benefit-provisioning behaviors was mediated by reactive jealousy in women but not in men. The only partner effect that emerged from these analyses showed that BPF in women were negatively associated with the benefit-provisioning behaviors reported by their male partners. Discussion focuses on the implications of these results for the function that jealousy might serve in the strategies used by individuals with BPF to maintain their romantic relationships.

Backround: Depression is a significant concern in clinical and preclinical psychoneurobiological sciences due to its high prevalence and its individual and collective consequences. Identifying efficient biomarkers for accurate diagnosis is crucial, with ideal biomarkers having detectable serum levels and conformational and thermal stability. This study aims to identify stable plasma biomarkers for the diagnosis and prognosis of major depressive disorder, as the pathogenesis of the disorder remains incompletely understood, affecting diagnosis accuracy. Methods: Thus, this study included ten MDD patients and eight healthy controls. The present work analyzed miRNAs in patients with major depressive disorder compared to healthy controls. Results: Eleven specific miRNAs, particularly hsa-miR-874-3p; hsa-let-7d-5p; and hsa-miR-93-3p showed upregulation-type plasma variations in the group of patients with major depressive disorder. miRNA functionality is linked to depressive pathophysiology. Conclusions: This study identifies a “bouquet” of miRNAs with significant upregulation variations in patients with major depressive disorder, suggesting further research to determine their suitability for personalization and evaluation, ultimately becoming integral components of major depression serological evaluations.

Throughout their lifetimes, bridges are exposed to various forces that may lead to displacement and deformation, potentially compromising their structural integrity. Monitoring their structural integrity under environmental factors is essential for safety and maintenance under these conditions. This aspect is a fundamental component of Structural Health Monitoring (SHM). Many studies focus on Structural Health Monitoring (SHM), employing various theories, methodologies, and technologies that have advanced rapidly due to the expansion of information technology. The objective of this study is to pinpoint areas where research is lacking in the existing literature on the environmental factors that impact the displacement of bridges, along with the techniques and technology used to monitor these structures. To achieve this objective, the most critical environmental factors and technologies, particularly those that are sensor-based, have been identified through a systematic search of the most popular databases. Subsequently, the study utilized a bibliometric analysis, exploring the challenge and prospective research areas reflected in the specialized literature. The findings indicate a lack of scholarly investigation of environmental factors that influence the Structural Health Monitoring (SHM) of bridges, in particular studies regarding the effect of uneven sunlight on structures. The research provides a comprehensive understanding of the Structural Health Monitoring (SHM) of bridges and has practical implications for developing effective monitoring methodologies.

Aristolochic acids, compounds derived from Aristolochiaceae plant species, are associated with significant renal nephrotoxicity and carcinogenicity. Aristolochic acid I (AAI), the most predominant and potent of these compounds, is a primary etiological agent in acute and chronic kidney diseases such as Aristolochic Acid Nephropathy (AAN) and Balkan Endemic Nephropathy (BEN). Due to the kidneys’ critical role in xenobiotic excretion, they are the primary organs affected by AAI toxicity. Recent in vitro and in vivo studies have highlighted mitochondrial dysfunction as a crucial factor in the pathogenesis of these kidney diseases. This review provides an update on the recent advances in understanding the causes of acquired mitochondrial dysfunction within the context of AAN and BEN. Key findings include the identification of mitochondrial DNA depletion, loss of mitochondrial membrane potential, and decreased ATP production as significant contributors to kidney damage. Additionally, oxidative stress markers and inflammatory mediators have been implicated in disease progression. Potential therapeutic approaches, such as the use of antioxidants like vitamin C and catalpol, have shown promise in mitigating AAI-induced cytotoxicity. Furthermore, future predictive approaches like pharmacogenomics could pave the way for novel mitochondria-targeted treatments. A comprehensive characterization of mitochondrial function, its underlying molecular mechanisms, and specific biomarkers could offer valuable insights and potential therapeutic options, significantly impacting the current management of AAN and BEN.

Background and Objectives: Dentigerous cysts are one of the most frequent pathologies associated with unerupted or impacted teeth. Such cysts show a male predilection and a preference for the mandibular region. Also, they commonly occur in the second and third decades of life, with only 9% occurring in the first decade. The aim of this work is to apply and study the therapeutic algorithms developed for dentigerous cysts and their outcomes, from the early diagnostic stage to the complete healing phase of pediatric patients diagnosed with this medical condition. Materials and Methods: The study included 19 pediatric patients diagnosed with dentigerous cysts who underwent the enucleation and extraction or conservative attitude of the associated tooth. The bony healing was also followed-up 9 months after the surgery. Results: A higher incidence in the posterior area of the mandible and maxilla was observed, as well as a higher incidence in boys. The 9 months postoperative radiographic assessment showed that the bony defects were completely healed. Conclusions: A thorough understanding of the nature of the lesion backed by a good clinical history and by state-of-the-art radiographic and radiologic examinations can go a long way in helping the surgeon to choose the correct therapeutic approach and to ameliorate the medical condition in the best long-term interest of the young patient. The considered dentigerous cyst cases demonstrated that an early diagnosis and treatment of this pathology is followed by a responsive treatment.

Perinatal depression (PPD) presents a significant public health concern, often influenced by psychological and personality factors. This study investigated the impact of personality traits, particularly neuroticism, and obsessive-compulsive disorder (OCD) symptoms on the severity of PPD. The primary aim was to quantify the contributions of these factors to the risk and severity of PPD to enhance early intervention strategies. A total of 47 pregnant women with depressive symptoms per DSM-5 criteria at “Pius Brinzeu” County Emergency Hospital in Timisoara, Romania, were enrolled in this cross-sectional study, as well as 49 women without depressive symptoms as controls. Personality traits were assessed using the NEO Five-Factor Inventory (NEO-FFI), and OCD symptoms were measured using the Obsessive-Compulsive Inventory (OCI). Depression severity was evaluated using the Edinburgh Postnatal Depression Scale (EPDS). This set of questionnaires were administered antepartum and postpartum. The logistic regression analysis highlighted neuroticism as a significant predictor of PPD severity, with an increase in neuroticism associated with a higher risk of PPD (coefficient = 0.24, p < 0.001). Conversely, openness showed a protective effect (coefficient = −0.13, p = 0.009). Higher OCD symptomatology, particularly ordering and hoarding, were linked with increased depression scores. Specifically, the total OCI score significantly predicted the EPDS score (coefficient = 0.03, p = 0.003). Furthermore, significant increases in EPDS anxiety and depression scores were observed in the perinatal period, indicating worsening of symptoms (anxiety coefficient = 0.51; p < 0.001). The findings suggest that personality traits like neuroticism and OCD symptoms significantly contribute to the severity of PPD. Interventions targeting these specific traits could potentially mitigate the risk and severity of perinatal depression, underscoring the need for personalized treatment plans that consider these psychological dimensions.

A nanocrystalline Fe(Co) phase dispersed within an amorphous matrix was achieved after milling of Fe31Co31B30Ta8 powder mixtures up to 200 h by using a high-energy ball mill. The transformations occurring in the powders during milling were studied with the use of X-ray diffraction and scanning electron microscopy techniques. The transformation of the phase depends upon the milling time. With the increase of milling time, various nanostructured phases such as Fe(Co), Co2Ta, Co(Ta,B), fcc-Co, Fe-type borides, and amorphous phase were identified. Magnetic properties were also investigated and correlated with microstructural changes. By increasing milling time, irregular variation of saturation magnetization is identified in relation to the change in the composition of the system. Also, the gradual decrease in grain size to a few nanometers resulted in an increase in coercivity while increasing the milling time up to 200 h. The existence of non-magnetic Ta and B atoms in the neighboring Fe and Co atoms and the increase in the volume fraction of the amorphous phase are retained as the major factors of this hard magnetic behavior of the present system.

Nigeria sits at the crossroads of West and Central Africa; two increasingly critical regions for global health security. To strengthen cross-border collaboration for health security between its neighbors from West and Central Africa, the Federal Ministry of Health, Nigeria through the public health authority at the Points of Entry—Port Health Services, led the design of a multi-lateral framework for cross-border collaboration between Nigeria, the Republic of Benin, the Republic of Chad, the Republic of Cameroon and the Republic of Niger. This framework provides a platform for agreeing on bilateral national and district-level infectious disease surveillance information sharing as well as laying the groundwork for expanded collaboration in incident reporting, joint investigation and response across borders.

Recognizing the crucial role that transport and lifting installations play in various sectors, including industry, construction, and extraction, as well as in mechanizing loading and unloading operations, and the movement of equipment or materials, special attention must be oriented to the diversification and the production of these installations by using the most advanced technologies. The paper presents some operating principles and characteristics of a lifting and transport Spider Crane installation equipment. This installation is considered a more economical and efficient alternative to traditional cranes for works where lifting requirements are below certain limits and significant height. Thus, an analysis of the hydraulic lifting system was carried out, based on the calculation of hydraulic fluid flows for the drive pumps. Also, was evaluated the specific speeds and forces of the hydraulic cylinders, a mechanical analysis of the clamping system with hook, as well as a finite element analysis of some mechanical and hydraulic components.

The oral cavity’s soft and hard tissues create a conducive environment for microbial proliferation and biofilm development, facilitating the colonization of prosthodontic and implant materials such as titanium (Ti) and zirconia (Zr). This study aimed to compare the efficacy of conventional decontamination methodologies (i.e., chemical and mechanical, using 0.12% digluconate chlorhexidine (CHX) solution-treatment and airflow) to adjunctive laser-based interventions on Ti and Zr substrates inoculated with Staphylococcus (S.) aureus ATCC 25923. Additionally, this investigation sought to elucidate the impact of these treatments on temperature variations and surface integrity, analyzing the laser irradiation effects on these prevalent dental materials. Experimental configurations were delineated for both Ti and Zr samples across four groups: (1) a conventional treatment group (CV); (2) a photodynamic therapy group (PDT); (3) an Er:YAG laser treatment group (Er); (4) a combined PDT and Er:YAG treatment group (PDTEr). Also, a negative control group (C) that received no treatment was considered. The decontamination of the inoculated disc samples was evaluated by quantifying the microbial colonies in colony-forming units per milliliter (CFU/mL). Temperature variations on the surface of the samples were determined during laser treatments. Surface modifications were investigated using scanning electron microscopy (SEM) and optical coherence tomography (OCT). For statistical analysis, Fisher 95% confidence intervals, Hsu’s MCB method, and the Kruskal–Wallis test were applied. With regard to the 105 CFU/mL of the negative control group, results indicated average values equal for each study group to (1) 2.66 CFU/mL for Ti and 2 CFU/mL for Zr for the CV group; (2) 0.33 CFU/mL for Ti and 1 CFU/mL for Zr for the PDT group; (3) 1.25 CFU/mL for Ti and 0 CFU/mL for Zr for the Er group; (4), and 0 CFU/mL for both Ti and Zr for the PDTEr group. Therefore, the combined PDT and Er:YAG treatment (PDTEr) and the singular PDT modality outperformed conventional decontamination methods in eradicating S. aureus biofilms from both Ti and Zr surfaces. Notably, the PDTEr regime achieved a comprehensive elimination of microbial colonies on treated substrates. Surface examination employing OCT demonstrated discernible alterations in the surface morphology of samples subjected to Er:YAG and combined PDT and Er:YAG treatments. Temperature checks during treatments showed no major changes, suggesting the applied laser methods are safe. In conclusion, PDTEr and PDT eliminated bacteria more effectively, but Zr surfaces were more resilient, making them better for microbe-controlling applications. Also, the study demonstrated that the (less costly but lower resolution) OCT method can replace SEM for such investigations.

Posture results from intricate interactions between the central nervous system's control mechanisms, sensory integration, and musculoskeletal responses, encompassing two levels: sustaining tonic muscle activity for posture and making adjustments to maintain balance in response to disturbances. Due to its complexity, assessing posture with simple methods is challenging, necessitating the use of advanced techniques like stabilometric platforms to measure postural stability through the center of pressure oscillations, akin to an inverted pendulum concept, offering a comprehensive understanding of postural control mechanisms. Materials and Methods: The study aims to analyze the trajectory of the center of pressure (COP) in relation to the gender of the subjects, through directional indices (DI) and oscillation ratio (OR), in the process of posture maintenance. The study included a sample of 50 young subjects (21-24 years), sports practitioners, divided into two equal groups. Results: The study findings indicate that gender does not influence COP DI. However, there's a significant disparity between anteroposterior (AP) DI values (0.70) and mediolateral (ML) DI values (0.54). The results suggest a predominant reliance on AP control mechanisms for maintaining balance, regardless of gender. When visual input is absent, there's a slight increase in AP DI (0.72) and a decrease in ML DI (0.52), indicating a shift in balance strategies. In terms of OR, significant differences are observed based on gender, oscillation direction, and visual input. Female subjects exhibit higher AP OR (6.5) compared to males (5.1). Under closed eyes conditions, both genders show decreased AP OR (6.0 for females, 4.7 for males), indicating a reliance on alternative balance strategies in the absence of visual cues. Conclusion: The study highlights the complexity of postural control and empha-sizes the influence of factors like gender and visual input on postural stability metrics, providing valuable insights for further research and clinical practice. The analysis reveals that around 60% of COP oscillations occur in the AP direction, regardless of visual input changes. This underscores the critical role of AP control in maintaining balance, suggesting targeted interventions to enhance stability should prioritize this aspect.

Misophonia is a neurophysiological disorder with behavioral implications, is complex and multifactorial in origin, and is characterized by an atypical and disproportionate emotional response to specific sounds or associated visual stimuli. Triggers include human-generated sounds, mainly sounds related to feeding and breathing processes, and repetitive mechanical sounds. In response to the triggering stimulus, the patient experiences immediate, high-intensity, disproportionate physical and emotional reactions that affect their quality of life and social functioning. The symptoms of misophonia can occur at any age, but onset in childhood or adolescence is most common. Affected children live in a constant state of anxiety, suffer continuous physical and emotional discomfort, and are thus exposed to significant chronic stress. Chronic stress, especially during childhood, has consequences on the main biological systems through the dysregulation of the hypothalamic–pituitary–adrenal axis, including the gastrointestinal tract. Here, we provide arguments for a positive correlation between misophonic pathology and gastrointestinal symptoms, and this hypothesis may be the starting point for further longitudinal studies that could investigate the correlations between these childhood vulnerabilities caused by misophonia and their effect on the gastrointestinal system. Further research to study this hypothesis is essential to ensure correct and timely diagnosis and optimal psychological and pharmacological support.

Currently, allergen-specific immunotherapy (AIT) for ragweed allergy is still based on natural allergen extracts. This study aimed to analyse the ability of four commercially available AIT vaccines (CLUSTOID, TYRO-SIT, POLLINEX Quattro Plus and Diater Depot) regarding their ability to induce IgG antibodies against ragweed pollen allergens in rabbits. Accordingly, the IgG reactivity of AIT-induced rabbit sera was tested for ten different ragweed pollen allergens (Amb a 1, 3, 4, 5, 6, 8, 9, 10, 11 and 12) by an ELISA. Furthermore, the ability of rabbit AIT-specific sera to block allergic patients’ IgE binding to relevant ragweed allergens (Amb a 1, 4, 6, 8 and 11) and to inhibit allergen-induced basophil activation was evaluated by an IgE inhibition ELISA and a mediator release assay. Only two AIT vaccines (Diater Depot > CLUSTOID) induced relevant IgG antibody levels to the major ragweed allergen Amb a 1. The IgG responses induced by the AIT vaccines against the other ragweed allergens were low and highly heterogeneous. Interestingly, the kinetics of IgG responses were different among the AIT vaccines and even within one AIT vaccine (Diater Depot) for Amb a 1 (long-lasting) versus Amb a 8 and Amb a 11 (short-lived). This could be due to variations in allergen contents, the immunogenicity of the allergens, and different immunization protocols. The IgE inhibition experiments showed that rabbit AIT-specific sera containing high allergen-specific IgG levels were able to inhibit patients’ IgE binding and prevent the mediator release with Diater Depot. The high levels of allergen-specific IgG levels were associated with their ability to prevent the recognition of allergens by patients’ IgE and allergen-induced basophil activation, indicating that the measurement of allergen-induced IgG could be a useful surrogate marker for the immunological efficacy of vaccines. Accordingly, the results of our study may be helpful for the selection of personalized AIT vaccination strategies for ragweed-allergic patients.