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13 results on '"Verma, Angela"'

5. In-vitro blood-brain barrier models for drug screening and permeation studies: an overview

Author

Bagchi,Sounak, Chhibber,Tanya, Lahooti,Behnaz, Verma,Angela, Borse,Vivek, and Jayant,Rahul Dev

Subjects
Drug Design, Development and Therapy
Abstract

Sounak Bagchi,1 Tanya Chhibber,1 Behnaz Lahooti,1 Angela Verma,1 Vivek Borse,2 Rahul Dev Jayant1 1Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA; 2Centre for Nanotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, IndiaCorrespondence: Rahul Dev JayantDepartment of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, 1406 S. Coulter Street, Suite 1104, Amarillo, TX 79106, USATel +1 806 414 9154Fax +1 806 356 4034Email rd.jayant@ttuhsc.eduAbstract: The blood-brain barrier (BBB) is comprised of brain microvascular endothelial central nervous system (CNS) cells, which communicate with other CNS cells (astrocytes, pericytes) and behave according to the state of the CNS, by responding against pathological environments and modulating disease progression. The BBB plays a crucial role in maintaining homeostasis in the CNS by maintaining restricted transport of toxic or harmful molecules, transport of nutrients, and removal of metabolites from the brain. Neurological disorders, such as NeuroHIV, cerebral stroke, brain tumors, and other neurodegenerative diseases increase the permeability of the BBB. While on the other hand, semipermeable nature of BBB restricts the movement of bigger molecules i.e. drugs or proteins (>500kDa) across it, leading to minimal bioavailability of drugs in the CNS. This poses the most significant shortcoming in the development of therapeutics for CNS neurodegenerative disorders. Although the complexity of the BBB (dynamic and adaptable barrier) affects approaches of CNS drug delivery and promotes disease progression, understanding the composition and functions of BBB provides a platform for novel innovative approaches towards drug delivery to CNS. The methodical and scientific interests in the physiology and pathology of the BBB led to the development and the advancement of numerous in vitro models of the BBB. This review discusses the fundamentals of BBB structure, permeation mechanisms, an overview of all the different in-vitro BBB models with their advantages and disadvantages, and rationale of selecting penetration prediction methods towards the critical role in the development of the CNS therapeutics.Keywords: blood-brain barrier, BBB, brain microvascular endothelial cells, BMECs, tight junctions, TJs, proteins, central nervous system, CNS, induced pluripotent cells, iPSCs, in-silico prediction methods

Published
2019

8. Combined use of arginase and dichloroacetate exhibits anti‐proliferative effects in triple negative breast cancer cells.

Author

Verma, Angela, Hu, Xiaohui, Chen, Xin, Cheung, Edwin, Tam, Kin Yip, Lam, Yau‐Min, and Leung, Yun‐Chung

Subjects
*BREAST cancer, *ARGINASE, *MONOCARBOXYLIC acids, *CANCER cells, *COMBINATION drug therapy
Abstract

Objectives: Drug combination in cancer therapy aims to achieve synergistic therapeutic effect, reduced drug dosage, reduced drug toxicity and minimizes or delays the induction of drug resistance. In the present study, we investigated the anticancer effects of the combination of two metabolic modulators, dichloroacetate (DCA) and bacillus caldovelox arginase (BCA) (or pegyated human arginase (HA)). Methods: The combination treatments were evaluated in MCF‐7 and MDA‐MB 231 cells as well as in MDA‐MB 231 breast cancer xenograft model. Key findings: Dichloroacetate and BCA combination exhibited anti‐proliferative effects on MCF‐7 cells, which were found to be synergistic. Analysis of the gene expression upon drug treatments revealed that the synergistic anti‐proliferative effect on MCF‐7 cells was possibly in part due to the activation of the p53 pathway. A similar synergistic anti‐proliferative effect was observed in the combined use of DCA and HA on MCF‐7 and MDA‐MB231 cells, which was due to induction of cell cycle arrest at G2/M phase. Moreover, the combination enhanced anti‐tumour activity in a MDA‐MB 231 xenograft mouse model. Conclusions: Our results suggested that dichloroacetate and arginase combination exhibited enhanced anti‐cancer effects in preclinical breast cancer models which may offer an additional treatment option for breast cancer. [ABSTRACT FROM AUTHOR]

Published
2019
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13. Probiotics Lactobacillus rhamnosus GG , Lactobacillus acidophilus Suppresses DMH-Induced Procarcinogenic Fecal Enzymes and Preneoplastic Aberrant Crypt Foci in Early Colon Carcinogenesis in Sprague Dawley Rats.

Author

Verma, Angela and Shukla, Geeta

Subjects
*ENZYMES, *THERAPEUTIC use of probiotics, *FECES, *ANIMAL experimentation, *CARCINOGENS, *COLON tumors, *IMMUNOENZYME technique, *LACTOBACILLUS acidophilus, *RATS, *PHYSIOLOGY
Abstract

Diet makes an important contribution to colorectal cancer (CRC) risk implying risks for CRC are potentially reducible. Therefore, the probiotics have been suggested as the prophylactic measure in colon cancer. In this study, different probiotics were used to compare their protective potential against 1,2 dimethylhydrazine dihydrochloride (DMH)-induced chemical colon carcinogenesis in Sprague Dawley rats. Animals belonging to different probiotic groups were fed orally with 1 × 109lactobacilli daily for 1 week, and then a weekly injection of DMH was given intraperitoneally for 6 wks with daily administration of probiotic.Lactobacillus GGandL.acidophilus+ DMH-treated animals had maximum percent reduction in ACF counts. A significant decrease (P< 0.05) in fecal nitroreductase activity was observed inL.casei+ DMH andL.plantarum+ DMH-treated rats whereas β-glucuronidase activity decreased inL.GG+ DMH andL.acidophilus+ DMH-treated rats. Animals treated withBifidobacterium bifidum+ DMH had significant decreased β-glucosidase activity. However, not much difference was observed in the colon morphology of animals belonging to various probiotic + DMH-treated rats compared with DMH-treated alone. The results indicated that probiotics,L.GG, andL.acidophiluscan be used as the better prophylactic agents for experimental colon carcinogenesis. [ABSTRACT FROM PUBLISHER]

Published
2013
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