Probiotics Lactobacillus rhamnosus GG , Lactobacillus acidophilus Suppresses DMH-Induced Procarcinogenic Fecal Enzymes and Preneoplastic Aberrant Crypt Foci in Early Colon Carcinogenesis in Sprague Dawley Rats.

Diet makes an important contribution to colorectal cancer (CRC) risk implying risks for CRC are potentially reducible. Therefore, the probiotics have been suggested as the prophylactic measure in colon cancer. In this study, different probiotics were used to compare their protective potential against 1,2 dimethylhydrazine dihydrochloride (DMH)-induced chemical colon carcinogenesis in Sprague Dawley rats. Animals belonging to different probiotic groups were fed orally with 1 × 109lactobacilli daily for 1 week, and then a weekly injection of DMH was given intraperitoneally for 6 wks with daily administration of probiotic.Lactobacillus GGandL.acidophilus+ DMH-treated animals had maximum percent reduction in ACF counts. A significant decrease (P< 0.05) in fecal nitroreductase activity was observed inL.casei+ DMH andL.plantarum+ DMH-treated rats whereas β-glucuronidase activity decreased inL.GG+ DMH andL.acidophilus+ DMH-treated rats. Animals treated withBifidobacterium bifidum+ DMH had significant decreased β-glucosidase activity. However, not much difference was observed in the colon morphology of animals belonging to various probiotic + DMH-treated rats compared with DMH-treated alone. The results indicated that probiotics,L.GG, andL.acidophiluscan be used as the better prophylactic agents for experimental colon carcinogenesis. [ABSTRACT FROM PUBLISHER]