Search

Today, diabetes mellitus (DM) represents a problem on which the attention of endocrinologists, general practitioners, and dentists is focused. Early pathological changes, which manifest themselves in the oral cavity, determine the relevance and social significance of this pathology, because dentists are usually the first to diagnose disorders of carbohydrate metabolism based on the results of the analysis of specific complaints and typical clinical symptoms of damage to the mucous membrane and periodontal tissues. The aim of the work is to find out the pathogenetic characteristics of the reparative component of the post-extraction alveolar socket depending on the time after tooth extraction in experimental diabetes. Materials and methods. In total, 120 sexually mature (age 8–10 months) male Wistar rats, divided into 8 groups of 15 animals each, were used for research. Complex immunohistological, radiographic and biochemical studies were used for the purpose of detailed analysis of the features of the course and mechanisms of development of complications after tooth extraction against the background of DM. In order to assess the state of the innate link of local immunity, the specifics of the course of repair processes at different times of the post-extraction period, the levels of cell proliferation (by expression of Ki-67) and the concentration and content of CD68-positive macrophages in the mucous membrane were determined immunohistochemically. In order to determine the state of the bone tissue of the dental and jaw apparatus and the levels of mineralization of the hard tissues of the molars in the animals of the experimental groups, radiovisiographic images of the right half-mandible were analyzed with determination of the radiographic density. Results. Analysis of proliferative-reparative relationships showed almost no macrophages and very low proliferative activity in the intact periodontium, on the other hand, in DM, initial significant diffuse infiltration by CD68-immunopositive cells with high levels of Ki-67 expression was found. Analysis of X-ray images of the half-mandible revealed that induced DM in rats, even without prior surgical intervention, led to the intensification of resorption processes in bone tissue, which was evidenced by a decrease in radiographic density in the root region of the 1st molar and intraalveolar bone membranes of all three lower molars. Conclusions. In the intact periodontium, there are almost no CD68-immunopositive macrophages and very low proliferation activity in the periodontal soft tissues. In experimental diabetes, on the contrary, initial significant diffuse infiltration by CD68-immunopositive macrophages with high proliferative activity of most fibroblasts is determined. Induced diabetes in rats, even without prior surgical intervention, leads to increased resorption processes in the bone tissue of the mandible and long-term prolongation of the inflammatory process after tooth extraction.

The aim of the study is to develop a questionnaire integrating traditional career guidance techniques and categorization of IT professions by analyzing existing classifications and interviewing IT specialists. Methods. In order to achieve the objective, we selected a traditional career guidance methodology by analysing the existing ones and selecting the one that best corresponds to the research objective; we also selected a system of categorisation of IT specialties by analysing the existing classifications and interviewing IT specialists. Results. Among the existing traditional career guidance techniques, the Holland Test was selected as having the potential to be adapted to the IT field. The analysis of the existing classifications of IT specialities showed the lack of unity, in this context our own categorisation was developed, including five main categories: development, QA specialists, working with data and research, management, design. Conclusions. Based on the results of the study, it was hypothesised that there are differences in the degree of expression of the Holland types among the different IT professions. Also, the presumed correspondence between different categories of IT professions and the predominant Holland types is given. The study forms the basis for the development of a software product that will help people to identify the most suitable IT professions for them.

Relevance. The need to assess the stability of combustion, thermal stress and nitrogen oxides (NOx) emission during load reduction of a steam boiler. Since renewable energy sources and nuclear power plants will receive great attention in the future, coal-fired thermal power plants are to operate at reduced loads, so it is important to investigate the reliability of operation and environmental parameters of the boiler unit in case of load adjustment. Aim. To investigate pulverized coal fuel burnout, temperature parameters and NOx emission at 50 % reduction of boiler unit load in the base configuration and taking into account installation of tertiary blast nozzles. Objects. Furnace chamber of a natural circulation boiler unit with steam capacity of 220 t/h in the baseline layout and with tertiary air nozzles. Methods. The package of application programs FIRE-3D for numerical study was applied. Combustion of pulverized coal fuel is a complex physical and chemical process, therefore the interaction of gas flow and solid particles was modeled using Eulerian and Lagrangian schemes, respectively. In the gas phase, the combustion of volatiles and CO with further combustion of carbon residue are modeled. NOx emission is modeled using post-treatment models including formation of fast, fuel and thermal nitrogen oxides. Results. Temperature fields, flow characteristics, NOx emissions for different loads of the furnace chamber of the boiler unit with steam capacity of 220 t/hour are obtained on the basics of numerical modeling. The authors have obtained quantitative estimations of furnace environment parameters corresponding to several levels of boiler load reduction up to 50% of the nominal one. Installation of four tertiary blast nozzles allows reducing NOx emissions by 12.75% at theoretically required amount of air in burner devices (α=1.0).

Mechanisms of beta-endocrinocyte differentiation involving c-kit may be associated with a number of processes, including signaling pathways and regulatory mechanisms. Such factors as activation of the c-kit signaling pathway, an increase or decrease in cell proliferation and survival, regulation of differentiation gene programs and stem cells are important in controlling self-preservation and regulating the cellular number and types, that are differentiated being impacted by intermittent hypoxic hypoxia, hypertension and diabetes mellitus. The aim of the work is to determine the dynamics of c-kit proliferative factor in beta cells under an exogenous influence (intermittent hypoxic hypoxia) and endogenous pathology (arterial hypertension and diabetes mellitus). Materials and methods. The study was conducted using 15 white Wistar rats and 5 spontaneously hypertensive rats (SHR), which were divided into 4 groups of 5 animals each. Group 1 – control (intact) rats, group 2 – animals with experimental diabetes mellitus, group 3 – rats with hereditary arterial hypertension (SHR), group 4 – animals exposed to the effect of intermittent hypoxic hypoxia. At the end of the experiment, the animals were euthanized, organs were harvested and processed, serial histological pancreatic sections 5 µm thick were deparaffinized and retrieved. Insulin and c-kit+ beta progenitor cells were detected by the immunofluorescence method using antibodies. Immunofluorescent reactions were studied using an AxioImager-M2 fluorescence microscope. Results. The quantitative indicator of beta cells was almost 6 times decreased in rats with diabetes mellitus. Concentrations of insulin in animals of this group were increased compared to intact animals and the marker of proliferative activity was decreased without changes in the percentage of c-kit-immunopositive beta cells. The course of hereditary arterial hypertension in SHR was accompanied by an 8-fold decrease in the number of beta cells per 1 cm2, an increase in insulin in beta cells as compared to the animals of both intact and experimental diabetes groups. Regarding the c-kit expression in beta cells, it was significantly reduced only in the group of intact animals and the number of c-kit-immunopositive beta cells was almost 2.8 times decreased. In the experimental group of animals exposed to hypoxic hypoxia, the number of beta cells per measurement unit was significantly increased, the concentration of insulin in beta cells was increased as compared to the intact animals, but lower than that in rats with diabetes and hypertension. The marker of proliferative activity c-kit was decreased compared to both intact and diabetic animals. As for the number of c-kit-immunopositive beta cells, it did not differ statistically from that in intact and diabetic animals, but was higher than that in SHR. Conclusions. The development of experimental diabetes in Wistar rats and hereditary hypertension in SHR results in a significant decrease in the specific density of beta cells in the pancreas in contrast to the effect of multiple-day hypoxic training, which stimulates an increase in the density of insulin-synthesizing endocrinocytes. Experimental conditions, diabetes mellitus, hypertension and adaptation to hypoxia, are accompanied by an increase in insulin and a decrease in the c-kit expression in beta cells. However, the number of c-kit-immunopositive beta cells in the pancreatic islets significantly decreases only with the development of hereditary arterial hypertension.

Express methods for estimating the intensity of heat treatment of milk are necessary in industry and in research work. For this reason, there are many ways to measure this parameter, which are based on different physical principles, including turbidimetric methods. The Harland & Ashworth turbidimetric method has been developed for a long time, however, due to its high reliability and ease of implementation, it is still used in practice. However, this method has a drawback: it takes a long time to perform the analysis. In this regard, the aim of the work is to develop an express method for evaluating the thermal class of milk based on the principle of measuring the concentration of soluble whey proteins. The result is achieved through the use of a turbidimetric measurement method with optimized sample preparation parameters and parameters for measuring the optical density of a suspension of protein aggregates. The method is implemented as follows. The milk sample is mixed with 0.1 N acetate buffer (pH 4.6) in a ratio that allows to obtain a concentration of soluble milk whey proteins from 0.05% to 0.1%. Recommended dilution coefficients: 1:3 for samples of ultra-pasteurized milk and pasteurized milk with high heat treatment intensity; 1:7 for samples of pasteurized milk with low heat treatment intensity and 1:14 for raw milk samples. The solution is filtered on a membrane filter with a pore size of 0.45 microns. The resulting filtrate is mixed with 24% trichloroacetic acid (TCA) in a 1:1 ratio to coagulate soluble whey proteins and form protein aggregates. After holding for 5–10 minutes, the optical density of the suspension of protein aggregates is measured at a wavelength of 650 nm. The content of water-soluble whey proteins in the sample can be calculated according to the calibration curve. The developed method allows to obtain the measurement result in less time than the Harland & Ashworth turbidimetric method.

In modern medical science great attention is paid to the clarification of the molecular mechanisms, which are the basis of adaptation to environmental factors of unusual origin and/or extraordinary strength. The aim of the study is to determine the features of a group of genes with low expression level, associated with hypoxia in the pancreas of Wistar rats under conditions of intermittent hypoxia. Materials and methods. The study was conducted on 10 white, sexually mature Wistar rats, which were divided into 2 groups (5 animals in each). Animals of group 1 were part of the control (intact) group. The animals of the 2nd group were subjected to hypoxic training according to the following scheme: for 15 days, 6 hours daily, namely on days 1–5 they simulated an ascent to a height of one to five kilometers above sea level under the conditions of a barometer, and the last 10 days 6 km above the sea level. To analyze gene expression, we used the polymerase chain reaction method with real-time reverse transcription (PCR) CFX-96 Touch™ (Bio-Rad, USA) and the RT2 Profiler™ PCR Array Rat Hypoxia Signaling Pathway kit (QIAGEN, Germany), where 84 genes were the subject of research in experimental animals. Results. According to the results of the PCR study of genes in the pancreas samples of intact animals and animals exposed to hypoxic training, it was established that out of 84 genes associated with hypoxia, a group of 5 genes with a low expression level (∆∆Ct < 30) was found. This pattern includes Bhlhe40 genes, Ctsa, Hif1a, Lox, and Slc16a3, the expression of which is statistically reduced. Thus, compared to the level of their expression in intact animals, the expression of Bhlhe40 decreased by 2.59 times, Ctsa by 6.02 times, Hif1a by 3.85 times, Lox by 3.01 times, and Slc16a3 by 2.40 times. Conclusions. Intermittent hypoxia reduces the expression of the Bhlhe40 gene by 2.59 times, which can be considered as an element of adaptation of cells to a low level of oxygen and modulation of genetic programs. The decrease in Ctsa gene expression by 6.02, Hif1a by 3.85, and Lox by 3.01 times during intermittent hypoxia demonstrates, that these effects can be used as sanogenic factors in insulin resistance and type 2 diabetes. The 2.40-fold decreased expression level of Slc16a3 is probably an element of metabolic adaptation and adaptation of the metabolic pathway of cells to hypoxia conditions.

With the rapid development of Internet technologies, the problems of network security continue to worsen. So, one of the most common methods of maintaining security and preventing malicious attacks is CAPTCHA (fully automated public Turing test). CAPTCHA most often consists of some kind of security code, to bypass which it is necessary to perform a simple task, such as entering a word displayed in an image, solving a basic arithmetic equation, etc. However, the most widely used type of CAPTCHA is still the text type. In the recent years, the development of computer vision and, in particular, neural networks has contributed to a decrease in the resistance to hacking of text CAPTCHA. However, the security and resistance to recognition of complex CAPTCHA containing a lot of noise and distortion is still insufficiently studied. This study examines CAPTCHA, the distinctive feature of which is the use of a large number of different distortions, and each individual image uses its own different set of distortions, that is why even the human eye cannot always recognize what is depicted in the photo. The purpose of this work is to assess the security of sites using the CAPTCHA text type by testing their resistance to an automated solution. This testing will be used for the subsequent development of recommendations for improving the effectiveness of protection mechanisms. The result of the work is an implemented synthetic generator and discriminator of the CGAN architecture, as well as a decoder program, which is a trained convolutional neural network that solves this type of CAPTCHA. The recognition accuracy of the model constructed in the article was 63 % on an initially very limited data set, which shows the information security risks that sites using a similar type of CAPTCHA can carry.

The study examined semi-hard cheeses made with milk-clotting enzymes (MCEs) of animal origin (Naturen Extra with a mass fraction of chymosin 95%, “Bovine Pepsin” with a mass fraction of chymosin 10%), microbial origin (Fromase 750 XLG) and recombinant origin (Chy-max Extra and Chy-max Supreme), at an introduction dose of MCE of 1,500 to 6,000 IMCU per 100 kg of milk. In cheeses at the age of 7, 60, and 150 days, pH, the degree of proteolysis, the content of peptides with a mass of 1–5 kDa, and the compressive stress at fracture were determined. Cheeses produced with a dose of MCE of 5,000–6,000 IMCU/100 kg of milk had a substantially (p0.05) in the degree of proteolysis between cheeses made using diverse types of MCEs at the same dose. The exceptions were cheeses produced with Chy-max Supreme, which had a substantially (p

Intermittent hypoxia affects a variety of pathological conditions in the body and is used in medicine to reproduce the sanogenic therapeutic effect. Intermittent hypoxia is used for clinical indications to improve lung function; increase the body’s adaptive capacity; in obstructive sleep apnoea syndrome; in anaemia, diabetes mellitus. In cancer radiotherapy, intermittent hypoxic training helps to increase the sensitivity of the tumour to treatment. Intermittent hypoxia is useful in rehabilitation medicine to improve the physical recovery of patients after surgery or injury to improve the body’s functional capabilities. However, to date, the activity of regulatory genes, that activate the molecular mechanisms of the above-mentioned sanogenic effects of intermittent hypoxia has not been sufficiently studied. The aim of the study: to determine the expression of hypoxia-related genes in pancreas of Wistar rats under intermittent hypoxia. Materials and methods. The CFX-96 Touch™ real-time reverse transcription polymerase chain reaction (PCR) (Bio-Rad, USA) and the RT2 Profiler™ PCR Array Rat Hypoxia Signalling Pathway kit (QIAGEN, Germany) were used to analyse gene expression in experimental animals, where 84 genes involved in the hypoxia signalling pathway identified in the pancreas were studied. Results. According to the results of PCR analysis of pancreatic samples from intact animals and animals exposed to hypoxic training, the activity of the gene panel can be distributed as follows: genes with high expression compared to the intact group of animals, genes with low expression compared to the intact group of animals and genes in which no significant changes were detected in the samples compared to the intact group of animals. We have found, that the genes Cops5, F10, Jmjd6, Lgals3, Rbpjl, Vegfa have high expression activity compared to the intact group of animals. Conclusions. The increase in the expression of Cops5 by 10.29 times, Lgals3 by 2.94 times and Rbpjl by 5.73 times as a result of intermittent hypoxia can be regarded as an activating factor for the increase in the proliferation of endocrine and exocrine pancreatic cells and the growth of their antiapoptotic potential. The 3.20-fold increase in Jmjd6 gene expression under intermittent hypoxia can be interpreted as a regulatory effect through protein dehydroxylation and promotion of antiapoptotic protein activity in endocrine and exocrine pancreatic cells. The 5.99-fold increased level of Vegfa expression may promote angiogenesis in the pancreas under intermittent hypoxia.

The purpose of the research is to determine main toxicological parameters in repeated intragastric administration of Altric-Extra for veterinary use to laboratory outbred rats.Materials and methods. White outbred rats were used as a test system in the study. The experiment used clinical, toxicological, biochemical and morphological research methods.Results and discussion. Altric-Extra doses of 325 and 650 mg/kg were toxic for the laboratory animals. When administered daily intragastrically to the rats in the above doses, the drug had a negative effect on the functional status of the hemopoietic organs, and excretory and digestive systems. The main target organ for this drug is the thymus. Functional disorders in the above organs and systems are irreversible as evidenced by the post-mortem examination, general blood analysis, and biochemical blood test obtained 10 days after the drug administration was completed.

Sand cats, Felis margarita, range from northern Africa and the Arabian Peninsula to Central Asia. Their apparently discontinuous distribution is recognized as comprising four subspecies. Recent genetic research found little differentiation between subspecies except for the North African form. In this study, 90 skins and 88 skulls were analyzed from the four subspecies. A discriminant function analysis of the scores, ranging from 1 to 4, of four pelage characteristics revealed differentiation between putative subspecies, except between Turkmenian and Pakistani sand cats. Northern African and Arabian sand cats tend to be spotted and striped, while Turkmenian and Pakistani sand cats are less spotted and have a dorsal crest of fur. Nonmetric multidimensional scaling (NMDS) models generated from 21 skull measurements revealed an overlap in morphospace between all subspecies, except for larger Turkmenian sand cats; northern African sand cats were smallest. Therefore, both pelage characteristics and skull morphometrics support up to three subspecies. However, considering recent genetic research, it is likely that two subspecies should be recognized, F. m. margarita from northern Africa and F. m. thinobia from the Arabian Peninsula, and Southwest and Central Asia. Widening of the dataset and nuclear DNA evidence are required to increase our understanding of geographical variation in this little studied species.

Background. The analysis of destabilizing factors and random processes of degradation of parameters of engineering devices and systems as the causes of parametric failures is carried out. Materials and methods. The main attention is paid to the synthesis of mathematical models that are advisable to use within the framework of the functionalparametric direction of reliability theory. Results and conclusions. The proposed mathematical models can serve as the basis for solving problems of reliability and safety management of complex systems of responsible purpose.

The article assesses concomitant diabetes mellitus as a risk factor for venous thromboembolic complications in patients with covid-19 in hospital settings. We analyzed 123 case histories of patients hospitalized for new coronavirus infection in the first wave of COVID-19. The results showed that diabetes mellitus was not an independent risk factor for venous thromboembolic complications in patients with COVID-19.

Perforated duodenal ulcer is a common pathology with fairly high postoperative mortality, especially in late diagnosis. In some cases, timely diagnosis of a perforative ulcer may be difficult and the results of the survey may be contradictory. The classical triad of clinical signs of perforative ulcers is not always observed in patients, sometimes the clinical picture is so unclear that the final diagnosis can only be made intraoperative. The article presents an analysis of the clinical case of difficult diagnosis and treatment of perforative duodenal ulcer in a patient in an acute period of ischemic stroke. In addition, the patient had severe concomitant somatic pathology with a high risk of systemic thromboembolism and at the same time a high risk of bleeding.

The possibility of using digital traces of online social network users, using community themes as an example, to support decision-making in career guidance diagnostics is investigated. Statistical analysis was performed: descriptive statistics, z-criterion for comparing two groups, and regression analysis. The themes of users’ subscriptions to various communities available in the social network as well as the gender of the respondent and the number of friends in the social network indicated in the profile were analysed as digital user traces. The socio-professional orientation of the personality was assessed based on the results of the Holland test (edited by G.V. Rezapkina). The correlation between users’ digital traces expressed by the themes of subscriptions, and key indicators of socio-professional orientation reflected in the results of the Holland test was analyzed based on the pilot study conducted through an online social networking application. The statistical analysis confirmed the hypothesis that user interests, in the form of community themes, are related to the results of the Holland test. The hypotheses of existing differences in the groups of men and women in the studied attributes (the results of the Holland test and the leading themes of community subscriptions) were proved. By means of regression analysis among the group of women the correlation was found between the prevalence of the community theme “Education” and the key indicators: A (Artistic), E (Enterprising), I (Intellectual); prevalence of the theme “Lifestyle” and severity of the indicators: C (Conventional), I, A, E; “Mass Media” and indicator C. Among the group of men, a correlation was found between the prevalence of “Sports” subject matter and indicator E. The results of the work expanded the space of potential predictors of users’ vocational orientation. A foundation for large-scale research in quantifying and constructing predictive models of key occupational indicators based on users’ subscription topics has been obtained. The results are useful in the direction of developing an integrated approach to creating a recommendation system for user career guidance.

Relevance. The need to analyze the furnace processes in boiler units with vertical vortex when arranging pulverized coal combustion with the enhancement of environmental parameters through the installation of tertiary blast nozzles. Despite the carbon-free policy in modern power engineering coal remains one of the main sources of energy, so the reconstruction of boiler units in order to reduce harmful emissions is very relevant. Aim. To study aerodynamics and combustion processes of pulverized coal fuel in the furnace chamber of a boiler unit with a tangential arrangement of burner devices using a combustion scheme with tertiary air blowing. Methods. The Eulerian–Lagrangian approach was applied to numerical study of flow characteristics, heat transfer and combustion in a furnace with a tangential burner arrangement. Also, k-ε model of gas turbulence, particle-turbulence interaction, diffusion model of particle dispersion, P-1 radiation modeling method and pulverized coal particle combustion model based on global particle kinetics and experimental data were used for numerical calculations. Results. Based on numerical calculations and analysis, the dependences of combustion product concentrations, temperature, hydrodynamics at combustion of Kuznetsky coal grade D in the furnace chamber with tangential arrangement of burners for brown coal, as well as at installation of tertiary blast nozzles have been obtained. Insufficient redistribution of air between burners and tertiary nozzles is revealed, as for maintenance of stable twist of vertical vortex and accordingly presence of high values of velocities at the outlet of burner devices it is not possible to increase the portion of tertiary air without reconstruction of burners. It is also established that the volume of the furnace chamber below the burners is poorly involved in heat exchange.

The effect of milk-clotting enzymes (MCEs) of animal origin (Naturen Extra with a mass fraction of chymosins of 95%, “Bovine Pepsin” with a mass fraction of chymosin of 10%), as well as MCEs of microbial origin (Fromase 750 XLG) and recombinant origin (Chy-max Extra and Chy-max Supreme) on the duration of milk coagulation and processing of cheese curd, as well as on the composition of whey and fresh cheeses in the manufacture of semihard cheeses, at an introduction dose of MCE of 1,500 to 6,000 IMCU per 100 kg of milk. Increasing the MCE dose from 1,500 to 2,000–3,000 IMCU/100 kg of milk leads to a decrease in the coagulation duration by an average of 20 min (p0.05). The use of MCEs of Naturen, Chy-max Extra and Chy-max Supreme brands at a dosage of 5,000–6,000 IMCU/100 kg of milk gives an average of 1.5% higher actual cheese yield compared to MCEs of the Fromase and “Pepsin” brands at an introduction dose of 1500 IMCU/100 kg of milk.

We investigated the possibility of automating the prediction of the 16-factor personality traits by R. Cattell from text posts of social media users. The proposed new method of automating the evaluation of R. Kettell’s 16-factor personality test traits includes language models and neural networks. Implementation of the method involves several steps. At the first step text posts are extracted from user accounts of social media, pre-processed with language model RuBERT and previously trained over a full-connected neural network. The result of this step is a normalized empirical distribution of the posts by the previously introduced classes for each user. Subsequently, based on the distribution of user posts the evaluation of the expression of psychological features of the user is made with the help of support vector machine, random forest and Naive Bayesian classifier. The final data set for model building and further testing their performance was made up of 183 respondents who took the R. Cattell test, with links to their public social media accounts. Classifiers predicting results for six factors (A, B, F, I, N, Q1) of R. Cattells 16-factor personality test were constructed. The results can be used to create a prototype of automated system for predicting the severity of psychological features of social media users. Results of work are useful in the applied and research systems connected with marketing, psychology and sociology, and also in the field of protection of users from social engineering attacks.

Вступ. Проведений семантичний аналіз відповідних джерел інформації щодо проблеми вторинної профілактики захворювань у військовослужбовців дозволяє констатувати, що інформаційні матеріали віддзеркалюють показники їх захворюваності з актуальних патологічних станів, констатують їх негативних вплив на боєздатність військовослужбовців, обґрунтовують необхідність удосконалення заходів профілактичного впливу з використанням підходів пацієнт-орієнтованої медичної допомоги, але не враховують в повному обсязі особливостей їх проведення в умовах воєнного стану. Виходячи з цього, проблему розробки уніфікованої організаційної моделі проведення вторинної профілактики захворювань військовослужбовців, яка б передбачала ефективну взаємодію військових та цивільних медичних закладів в межах єдиного медичного простору за умов дії правового режиму воєнного стану, слід вважати актуальною, що й дозволило визначити мету проведеного дослідження. Мета дослідження полягає у обґрунтуванні уніфікованої моделі організації проведення вторинної профілактики захворюваності серед військовослужбовців в умовах дії правового режиму воєнного стану, яка передбачає забезпечення ефективної взаємодії військово-медичних закладів з закладами охорони здоров’я МОЗ України в цих умовах. Матеріали та методи. Дослідження проводилося із використанням класичних соціально-гігієнічних методів: монографічного, семантичного, аналітичного, документального обліку та системного аналізу. Матеріалами дослідження слугували нормативні документи та інформаційні матеріали з питань профілактики та захворюваності військовослужбовців. Результати. У статті обґрунтована уніфікована модель організації проведення вторинної профілактики захворюваності серед військовослужбовців в умовах дії правового режиму воєнного стану, яка передбачає забезпечення ефективної взаємодії військово-медичних закладів з закладами охорони здоров’я МОЗ України в цих умовах. Проведено обґрунтування, визначені організаційні підходи та шляхи удосконалення заходів з вторинної профілактики захворюваності військовослужбовців. Висновки. Доведено, що від якісного проведення вторинної профілактики захворювань залежить забезпечення здоров’я військовослужбовців, які мають значний досвід бойових дій, а, відповідно, сприяють підвищенню боєздатності військових частин і підрозділів. Розроблена уніфікована модель взаємодії військово-медичних закладів з закладами охорони здоров’я МОЗ України в умовах воєнного стану. Впровадження заходів вторинної профілактики сприятиме попередженню загострення хронічних захворювань тим самим зменшуючи функціональне навантаження на військово-медичні заклади, що має актуальне значення в умовах воєнного стану. Слід вважати, що проведення вторинної профілактики військовослужбовців не повинно підміняти заходів первинної профілактики захворювань та травм, з урахування особливостей військової служби в умовах воєнного стану.

The effect of the type (bovine chymosin (Chy-max Extra), camel chymosin (Chy-max M), and modified camel chymosin (Chy-max Supreme)) and applied dose (1500, 2500, or 3500 IMCU/100 kg of milk) of milk-clotting enzyme (MCE) on the proteolysis degree, microstructure, rheological and sensory properties of Crescenza soft cheese was studied. The proteolysis degree of cheeses was directly proportional to the general proteolytic activity (PA) and dose of the MCEs added into milk during the cheese production. With an equal dose of the MCEs added into milk, the highest level of proteolysis was noted in the cheese options produced with recombinant bovine chymosin (Chy-max Extra). There were no statistically significant differences (p > 0.05) in the proteolysis degree between the cheese options made with the same doses of the camel chymosin (Chy-max M) and modified chymosin (Chymax Supreme). After 21 days of storage, the cheeses with Chy-max Extra MCE had the most plastic consistency, while the cheeses with Chy-max Supreme MCE had the hardest and most elastic one. Cheeses produced with Chy-max M MCE occupied an intermediate position in terms of consistency density. Recombinant MCEs based on camel chymosin (Chy-max M) and modified chymosin (Chy-max Supreme) with low level of PA, may be recommended for use in the production of soft cheeses. This will extend the shelf life of cheeses by slowing-down the plasticization rate of their consistency.

Introduction: Despite the success which was achieved in the treatment of arterial hypertension, for optimization of the treatment, it is necessary to study the pathogenesis of primary arterial hypertension and target organ damage on the molecular level. Materials and methods: Our team studied the molecular mechanisms of myocardial damage during arterial hypertension and metabolic disorders. We used the spontaneously hypertensive rats (SHR) as an experimental model, and, additionally, we modeled diabetes mellitus and atherosclerosis in these rats. Results and discussion: Our study obtained evidence of a much higher level of the energy imbalance in the cardiomyocytes and more intensive production of reactive oxygen species in the SHRs with diabetes mellitus and atherosclerosis compared with the healthy animals and the animals with only hypertension. The indicated defections create an environment for further cellular damage – mitochondrial dysfunction, depletion in the thiol-disulfide system, and formation of highly reactive NO products. At the same time, we have noticed a higher activity of the Hsp70 in the hypertensive groups compared with the normotensive animals. The source of these deviations is in the formation of mitochondrial dysfunction of cardiocytes, the cause of which is oxidative modification of the protein structures of mitochondria under conditions of activation of oxidative stress reactions, insufficiency of mPT pores, and impaired mitochondrial chaperone function. The presented data give reason to believe that mitochondrial dysfunction, which develops against the background of deficient HSP70, is an integral aspect of arterial hypertension, contributes to its aggravation, and triggers a cascade of molecular and biochemical mechanisms of myocardial damage. These mechanisms include disturbances in the L-arginine-NO-synthase-NO system, production of mitochondrial iNOS oxygen radicals, neutralization of the vasorelaxant effect of NO and its transformation into an active participant in nitrous stress due to reduced intermediates of the thiol-disulfide system. The question of cause-and-effect relationships of oxidative stress remains open for discussion. Conclusion: We envisage that studies in this direction may lead to a better insight into a pathogenetic therapy of essential hypertension, diabetes mellitus, and atherosclerosis. Graphical abstract:

Link for citation: Maltsev K.I., Gil A.V., Abramov N.V., Puzyrev S.A. Thermophysical and environmental assessment of natural gas utilization in the reconstruction of a pulverized coal-fired boiler. Bulletin of the Tomsk Polytechnic University. Geo Аssets Engineering, 2023, vol. 334, no. 8, рр. 30-38 In Rus. The relevance of the investigation is caused by the need to assess the completeness of fuel combustion, harmful emissions, and temperature stress of the screen surfaces of a pulverized coal-fired boiler during its reconstruction for natural gas combustion. Currently, the conversion of solid fuel combustion installations to natural gas is highly relevant due to significantly lower emissions of carbon compounds. The main aim of this research is to study the physicochemical processes in the combustion chamber at various loads with flammable natural gas combustion and evaluate the effectiveness of combustion organization during the reconstruction of a pulverized coal-fired boiler. Objects: combustion chamber of a boiler unit with a steam capacity of 210 t/h, burners, parameters of the combustion chamber environment Methods: comparison of results obtained from analytical thermal calculations and numerical modeling. The numerical simulation of gas flare combustion was performed using the ANSYS Fluent software package. Reynolds Averaged Navier–Stokes approaches based on averaging the Navier–Stokes equations over the Reynolds number with additional equations for the turbulent kinetic energy k and the rate of turbulent kinetic energy dissipation ε were applied to describe the turbulent flow. Results. A numerical study of the physicochemical processes in the combustion chamber of a boiler unit was conducted after its reconstruction for natural gas combustion. Dependencies of temperature level changes, hydrodynamics, and concentrations of combustion products components in the volume of the combustion chamber at various loads were obtained. It was found that high-temperature combustion products are redistributed towards the front and rear walls, resulting in the formation of zones in the wall layer with a significant temperature gradient. The nozzles of the tertiary air contribute to the reduction of nitrogen oxide emissions in the combustion chamber by suppressing their formation due to oxygen deficiency in the combustion zone, as well as by lowering the temperature of the flame in the oxidizing zone.

Вступ. Збільшення тривалості бойових дій на території України призведе до збільшення кількості військовослужбовців, які будуть потребувати проведення медико-соціальної експертизи та оформлювати для цього великий за змістом пакет медичної документації, що вимагає не тільки витрат часу, а це, в свою чергу, значно збільшить функціональне навантаження на систему військової та цивільної охорони здоров’я, що слід вважати неприпустимим у воєнний час, тому виникає нагальна потреба в оптимізації системи організації проведення медико-соціальної експертизи військовослужбовців в умовах воєнного стану, вищевикладене визначає актуальність проведеного дослідження і дозволяє сформулювати його мету. Мета дослідження полягає у розробці оптимізованої системи організації медико-соціальної експертизи військовослужбовців за рахунок впровадження мінімізованого переліку медичних документів, необхідного для її проходження військовослужбовцями (колишніх військовослужбовцями) в умовах воєнного стану. Матеріали та методи. Дослідження проводилось із використанням класичних соціально-гігієнічних методів: монографічного, семантичного, аналітичного, документального обліку та системного аналізу. Матеріалами дослідження слугували нормативні документи, що визначені для проходження медичної експертизи пацієнтів у МСЕК. Результати. У статті наведені результати розробки оптимізованої системи організації медико-соціальної експертизи військовослужбовців за рахунок впровадження мінімізованого переліку медичних документів, необхідного для її проходження в умовах воєнного стану. Доведено, що його впровадження прогнозовано дозволить оптимізувати процес проходження медико-соціальної експертизи, уникнути дублювання медичної документації для військовослужбовців, знизити зайве навантаження на цивільну систему охорони здоров’я, суттєво скоротити час підготовки медичних документів та знизити психоемоційне навантаження на військовослужбовців (колишніх військовослужбовців), пов’язане з підготовкою необхідних медичних та інших документів для скерування на проходження медико-соціальної експертизи у воєнний час (та період 6 місяців після його завершення). Розроблені нами пропозиції щодо оптимізованого переліку медичних документів для військовослужбовців для скерування на проходження медико-соціальної експертизи у медико-соціальних експертних комісіях МОЗ України були узагальнені, оформлені та направлені до Кабінету Міністрів України на розгляд та опрацювання. Висновки. Проведений семантичний аналіз нормативних документів щодо переліку медичної документації, яка подається для проходження медико-соціальної експертизи військовослужбовцями (колишніми військовослужбовцями), дозволяє вважати її виправданою для умов мирного часу. Проведеними дослідженнями визначена потреба у проведенні оптимізації переліку пакету медичних та інших документів для військовослужбовців (колишніх військовослужбовців) при їх скеруванні для проходження медико-соціальної експертизи у медико-соціальних експертних комісіях в умовах дії режиму воєнного стану. Під час дослідження обґрунтований оптимізований перелік медичних документів для військовослужбовців (колишніх військовослужбовців) незалежно від відомчого підпорядкування при скеруванні їх для проходження медико-соціальної експертизи у медико-соціальних експертних комісіях в умовах дії режиму воєнного стану. Встановлено, що впровадження та використання оптимізованого переліку медичних документів дозволить уникнути дублювання медичної документації для військовослужбовців, знизити функціональне навантаження на систему військової і цивільної охорони здоров’я під час дії воєнного стану та суттєво скоротити час підготовки медичних документів для військовослужбовців для проходження медико-соціальної експертизи під час дії режиму воєнного стану та протягом 6 місяців після його скасування.

The effect of milk-clotting enzymes (MCE) with different milk-clotting activity (MCA) and proteolytic activity (PA) based on recombinant calf chymosins (Chy-max® Extra: MCA=554 IMCU/g; PA=0.71 UA/g), camel (Chymax® M: MCA=904 IMCU/g; PA=0.68 PA units/g) and “modified” chymosin (Chy-max® Supreme: MCA=912 IMCU/g; PA=0.26 PA units/g) on the duration of milk coagulation, the composition of whey and fresh cheeses in the production of soft cheese of the Italian type Crescenza at a dose of MCE equal to 1500, 2500 and 3500 IMCU per 100 kg of milk was studied. With an equal introduction dose, the shortest average duration of curd formation is noted for Chy-max Supreme MCE (15.0–27.5 min), long one — for Chy-max M MCE (17.0–31.0 min), and the longest one — for MCE Chy-max Extra (18.0–35.5 min). There was no statistically significant effect (p< 0.05) of the type and dose of MCE on the total duration of cheese processing, as well as on the content of fat, protein and dry matter of whey. The type and dose of MCE had an impact on the properties of fresh cheeses: cheese options produced with the maximum dose of Chy-max Supreme had a statistically significant (p

Estimating word complexity with binary or continuous scores is a challenging task that has been studied for several domains and natural languages. Commonly this task is referred to as Complex Word Identification (CWI) or Lexical Complexity Prediction (LCP). Correct evaluation of word complexity can be an important step in many Lexical Simplification pipelines. Earlier works have usually presented methodologies of lexical complexity estimation with several restrictions: hand-crafted features correlated with word complexity, performed feature engineering to describe target words with features such as number of hypernyms, count of consonants, Named Entity tag, and evaluations with carefully selected target audiences. Modern works investigated the use of transforner-based models that afford extracting features from surrounding context as well. However, the majority of papers have been devoted to pipelines for the English language and few translated them to other languages such as German, French, and Spanish. In this paper we present a dataset of lexical complexity in context based on the Russian Synodal Bible collected using a crowdsourcing platform. We describe a methodology for collecting the data using a 5-point Likert scale for annotation, present descriptive statistics and compare results with analogous work for the English language. We evaluate a linear regression model as a baseline for predicting word complexity on handcrafted features, fastText and ELMo embeddings of target words. The result is a corpus consisting of 931 distinct words that used in 3,364 different contexts.

The study aims to establish the appropriate parameters of UV excitation intensity and permanent excitation time on the pancreatic islets photobleaching, the ratio of the intensity of the useful signal in the region of interest to the intensity of nonspecific background fluorescence. Materials and methods. The pancreas of three adult Wistar rats was fixed in Bouin solution (20 hours) and poured into paraplast after standard histological processing. The study was carried out on paraffin sections of the pancreas. The islets’ insulin and glucagon were determined by immunofluorescence method using monoclonal antibodies (Santa Cruz Biotechnology). The immunofluorescence reaction was studied using an AxioImager-M2 fluorescent microscope. AxioVision digital image analysis system was used for fluorescence imaging, and ImageJ 64-bit image analysis system was used for image quantification. 30 pancreatic islets with an area from 3000 µm2 to 5000 µm2 (8–13 % of the frame area) were analyzed. Results. Measurements carried out at constant values of hormone concentration in endocrinocytes showed a different estimate of the average fluorescence intensity for insulin and glucagon, which depended on the intensity of UV radiation. As the intensity of UV radiation increased, the average fluorescence intensity in the region of interest for insulin and glucagon increased, but when the camera exposure was corrected, it became almost the same. Regardless of this, the intensity of nonspecific background fluorescence increased monotonically. The use of the ratio of the logarithms of the background fluorescence of the drug and the fluorescence of endocrinocytes in the calculations gives a stable estimate of the relative concentration of hormones, which does not depend on the intensity of the selected UV radiation regime, as well as on the duration of UV irradiation of the drug. This makes it possible to neutralize the effect of photodynamic discoloration of the preparation caused by continuous irradiation. Methods for machine selection of the region of interest by various algorithms of the ImageJ program lead to different estimates of its area, integral, and average fluorescence values. At the same time, the result closest to the “ideal” interactive method of highlighting the area of interest for insulin and glucagon was shown by Otsu’s algorithm. Conclusions. In immunofluorescent examination of the pancreas, a moderate UV radiation mode should be selected, exposure correction of the CCD camera before taking each frame, and the total time for examining the visual field of the sample should be limited to 1–2 minutes. To highlight the area of interest for insulin and glucagon in automatic analysis, it is recommended to use the Otsu algorithm. To obtain a quantitative estimate of the average fluorescence intensity in the region of interest, it is recommended to use the ratio of the logarithms of the background fluorescence of the drug and endocrinocytes in the calculations.

Introduction. In recent years, ultrasound and endovisual guidance have been introduced into clinical practice when performing percutaneous nephrolitholapaxy.Objective. To identify the most significant factors that influence the frequency of complete elimination of stones and the development of complications in percutaneous nephrolitholapaxy performed under ultrasound and endovisual guidance.Materials and methods. We analyzed the results of the treatment of 515 kidney stone patients who underwent percutaneous nephrolitholapaxy under ultrasound navigation and endovideoscopic guidance using a new method developed by us.Results. The average duration of the operation was 77.2 ± 1.9 min. Most of the operations were performed through one puncture access (95.1%) and in one stage (91.8%) with complete stone elimination in 80.6% of the cases. The degree of decrease in hemoglobin in the postoperative period was only 12.18 ± 0.6 g/l. The frequency of postoperative complications was 29.3%, in most cases there were 1 - 2 severity grades according to Clavien-Dindo. As a result of multivariate regression analysis, independent predictors influencing the frequency of complete stone eradication were the number of stones (p = 0.012), the fact of the presence of staghorn stone (p = 0.016), the number of stages of surgical intervention (p = 0.001). Correlation analysis revealed a statistically significant negative correlation between body mass index and the occurrence of complications (p = 0.005), a positive correlation between the presence of urinary tract infection and the occurrence of complications (p = 0.048), a positive correlation between the grade of blood loss and the occurrence of expectation (р < 0.001).Conclusions. Percutaneous nephrolitholapaxy under ultrasound and endovisual guidance without the use of X-rays is an effective intervention for most patients with kidney stones. Complete stone eradication depends on the number of stones, the presence of staghorn stone, and the number of stages of surgery. The incidence of complications is significantly affected by the body mass index, the presence of urinary infection and the degree of decrease in hemoglobin during surgery.

The article presents the design of the architectural and artistic composition “Memorial stele on a local spherical support” (KB-1 Stele), as well as the analysis of design solutions and the calculated justification of the strength and stability of the KB-1 Stele to external influences in conditions of the most intense climatic influences with parameters set according to building codes and regulations. The design of the KB-1 Stele is a vertically installed overall mass model of a peaceful nuclear explosive device weighing 2.8 tons and 2.8 meters high with hemispherical covers. In accordance with the terms of reference, it was required to preserve the appearance of the device as accurately as possible by placing it on a small local support. The peculiarity of the design lies in its angular mobility relative to the support. To ensure a stable equilibrium state of the stele, a hinge is organized at the place of support, the stele is installed freely by the lower sphere on an intermediate stand with a specially selected spherical surface. Additionally, the center of mass of the stele is significantly reduced by lightening (thinning the walls) of the upper part of the body and installing additional cargo in its lower part. As a result, under the action of external lateral loads, the stele acquires a stable position with possible fluctuations in the tumbler type and with a return to its original state. Analytical and numerical computational studies were performed to substantiate the strength and stability of the KB-1 Stele under external loads. It is shown that the developed design meets the regulatory requirements and is safe in operation.

The ratio of the milk-clotting activity (MCA) and proteolytic activity (PA) was compared for milk-clotting enzyme preparations (MEP) based on recombinant chymosin, chymosin of animal origin and microbial origin. The MCA value was measured in the international milk-clotting units (IMCU), the PA value was determined by the Anson method on the substrate from hemoglobin at 30 °C and рН 5.3. It was found that recombinant chymosins had the highest level of MCA/ PA: the group with MCA/ PA < ~1000 includes MEPs Chy-max® Extra 2235 Powder, Chy-max® Extra 600 Liquid and Renifer® 1800; the group with ~1000 < MCA/ PA < 1400 includes Chy-max® M 1000, Maxiren® 1800 and Chymax® Extra 2235 Powder; the group with MCA/ PA > 3500 includes MEP Chy-max® Supreme 1000. MEPs of animal origin had a lower indicator of MCA/ PA compared to recombinant chymosins, which for different trademarks of MEPs with different ratios of%chymosin to%pepsin was equal to: Naturen® Extra 220 (95/5) = 199.7 ± 8.0; rennet SF “Extra” (90/10) = 122.1 ±1.9; Kalase® 150 (80/20) = 115.1 ± 0.7; Naturen® Stamix (50/50) = 86.2 ± 0.2; rennet FS10 “Bovine pepsin” (10/90) = 38.7 ± 1.8. MEP of microbial origin had the lowest level of MCA/ PA among all three types of MEPs under study. MCA/ PA for different trademarks of MEPs of microbial origin was as follows: Meito® Microbial Rennet = 36.35 ± 0.48; Marzyme® 2200 MT = 31.03 ± 0.13; Fromase® 2200 TL = 18.57 ± 0.13; Fromase® 750 XLG = 16.06 ± 0.49.

Mid IR Quantum cascade lasers are of high interest for the scientific community due to their unique applications. However, the QCL designs require careful engineering to overcome some crucial disadvantages. One of them is active region (ARn) overheating, which significantly affects laser characteristics, even in the pulsed mode. In this work, we consider the effects related to the nonequilibrium temperature distribution when thermal resistance formalism is irrelevant. We employ the heat equation and discuss the possible limitations and structural features stemming from the chemical composition of the ARn. We show that the presence of solid solutions in the ARn structure fundamentally limits the heat dissipation in pulsed and CW regimes due to their low thermal conductivity compared with binary compounds. Also, the QCL postgrowths affect the thermal properties of a device closer to CW mode, while it is by far less important in the short-pulsed mode.

A comparative test was carried out for milk-clotting enzymes (MCE) of animal origin (Naturen® Extra), microbial origin (Marzyme®) and MCE based on recombinant camel chymosin (Chy-max® M) in the production of soft cheese “Lyubitelskiy”. By the end of the shelf life of the cheeses (12 days at a temperature of 3 ± 1 °C), differences were noted in the degree of proteolysis (DP) and the value of the complex modulus G*, which were the following ones for cheeses produced with MCE of the brands: Naturen® — DP = 17.86 ± 0.24%; G* = 4164 ± 587 Pa; Marzyme® — DP = 17.98 ± 0.49%; G* = 4581±786 Pa; Chy-max® M — DP = 9.85 ± 0.63%; G* = 7949 ± 1157 Pa. Cheeses made with Chy-max® M MCE had a denser texture than cheeses made with MCE of Naturen or Marzyme, which did not differ significantly in consistency. In the studied cheeses, the severity of the bitter taste was proportional to the content of water-soluble peptides with a mass of 0.5–3 kDa. Cheeses with Marzyme® MCE had a more intense bitterness than cheeses with Naturen® MCE. There was no bitter taste in cheeses produced with MCE of Chy-max® M. It was concluded that in the production of soft cheeses, recombinant camel chymosin can be used to increase the shelf life, and MCE of microbial origin can be recommended to replace more expensive MCE of animal origin.

The article proposes a new approach to comparing accounts of the social media “VKontakte” and “Instagram” to determine those accounts which belong to the same user. The approach is based on the comparison of graphic content; the novelty of the approach consists in combining several methods for matching graphic content, also for the first time a method is proposed for matching accounts of the mentioned social media. The proposed method combines three methods of matching graphic content: by extracting the faces of the account users from the photos in the account and matching them, by matching all faces in both accounts, and by pairwise comparison of images to determine the same images in both accounts using the perceptual pHash method. The described method was tested on a dataset of more than 8,000 pairs of accounts. According to the results of the experiment, the value of the F1-score metric reached 0.87. The practical significance lies in automating the comparison of user accounts in various social networks by implementing of the developed algorithm in the prototype of the software package. A further direction for research lies in expanding the set of data and attributes of profiles considered for comparison. The results can be introduced into a software package for the analysis of the security of a user of information systems against social engineering attacks. It seems promising to combine the obtained findings with account matching methods based on the structural similarity of social graphs.

Bromide 1 - (β-phenylethyl)-4-amino-1,2,4-triazolium (Hypertril) has the properties of a beta-blocker and of NO-mimetic, is assigned to the IV class of toxicity. All these effects make Hypertril a promising drug for the treatment of cardiovascular diseases. The aim of this paper was to study the cardioprotective action of Hypertril in terms of the effect on the morpho-functional parameters of the myocardium in rats with experimental chronic heart failure (CHF). CHF was modeled on 80 white outbred rats weighing 190–220g by administering doxorubicin at a cumulative dose of 15 mg/kg. Hypertril and the reference drug metoprolol succinate were administered within 30 days after CHF modeling, intragastrically at doses of 3.5 mg/kg and 15 mg/kg. Morphometric analysis of the cellular structure of the myocardium was carried out on an Axioskop microscope (Zeiss, Germany), in an automatic mode using a macro program developed in a specialized programming environment VIDAS-2.5 (Kontron Elektronik, Germany). The administration of Hypertril to animals with CHF led to an increase in the density of nuclei of cardiomyocytes, the area of myocardiocyte nuclei, an increase in the nuclear cytoplasmic ratio and an increase in the concentration of RNA in the nuclei and cytoplasm of cardiomyocytes compared with the group of untreated animals, which indicated the presence of a pronounced cardioprotective effect in the drug candidate. In terms of such indicators as the density of surviving cardiomyocytes and the content of RNA in them, the nuclear-cytoplasmic ratio of Hypertril is significantly (p < 0.05) superior to metoprolol.

The paper considers the problem of evaluating frequency of the processes whose mathematical model is stochastic processes consisting of a series of sequential episodes with a known class of distributions of the length of the time interval between them. In the previously proposed approach, the input data included information about the value of the interval between the last episode and the end of the study period, which could lead to inaccurate results. This interval differs from the intervals between successive episodes, and hence its presentation and processing require approaches that take this feature into account. Accuracy of the estimation results for process frequency was improved by developing a new model based on the Bayesian confidence network that includes nodes corresponding to the intervals between the last episodes of the process, the minimum and maximum intervals between episodes, by correctly accounting for the values of the interval between the last episode and the end of the study period at the model training stage. The authors propose a Bayesian belief network that includes a random element characterizing the interval between the end of the study period and the last episode of the process during the study period; data on this interval can be available at the training stage. They used R programming and the bnlearn package to model the Bayesian belief network. A new approach to the estimation of process frequency based on the Bayesian belief network generated by machine learning methods is proposed. It allows increasing the accuracy of the results by correctly considering the value of the interval between the last episode and the end of the period under study using a special scheme in the machine learning Bayesian belief network which includes a “hypothetical” episode after the end of the study period. To test the proposed approach, data was collected on 5608 Instagram users, which included the time of posting for the year 2020 and the time of publishing the first post for the year 2021. 70 % of the sample was used to train the model, and 30 % was used to compare the posting frequency values predicted by the model with known values. The results can be used in various fields of science, where it is necessary to estimate a process frequency under information deficit, when the whole process is observed for no more than some limited time. Obtaining such estimates is often an important issue in medicine, epidemiology, sociology, etc. The approach shows good results on the comparison of the theoretical model and the results of learning from the social network data, which can automate the obtaining of process frequency estimates.

The purpose of this study is to study the modernization of the model of an attacker’s social engineering attack on a user, taking into account a wider range of factors influencing the success of a social engineering attack associated with the principles of social influence. Methods. To achieve this goal, the approaches to social influence and the components of social influence were analyzed. An integrated circuit of social influence is built, grounding in the context of socio-engineering attacks. Results. A model of social influence is proposed, built in the context of an attacker’s social engineering attack on a user. A new interpretation of the term user vulnerability in the context of information security has been proposed. Conclusion. The result obtained forms the potential of filling the user and attacker models with specific vulnerabilities and competencies, which will lead to a more accurate assessment of the success of the attacker’s social engineering attack on the user, due to the aggregation of information from incidents that have occurred.

The effect of the type of milk clotting enzyme (MCE) on the duration of milk coagulation, parameters of the composition of whey and cheeses, and the output of cheeses in the production of soft cheese such as “Lyubitel'skiy” were investigated. Three brands of MCE of different origins were investigated: Marzyme® (MCE of microbial origin based on Rhizomucor miehei protease), Naturen® (calf rennet) and Chy-max® M (recombinant camel chymosin). It was established that MCEs had different ratios of milk clotting activity (MCA) to total proteolytic activity (PA). It was determined that the MCA/PA ratio, which characterizes the degree of specificity of the MCE action with regard to kappa-casein, in Chy-max M 1000 is ~7 times higher than that of Naturen and ~50 times higher than that of Marzyme. Such differences did not lead to a negative effect when using the Marzyme preparation in the production of soft cheeses. There were no statistically significant differences in the amount of dry matter loss of the curd into the whey, physicochemical parameters and output between the variants of cheeses made with the studied brands of MCE. Shorter duration of milk clotting (16.5 min) was observed with Marzyme than with MCE of Naturen (20.5 min) and Chy-max M (22.5 min). The results of the coagulation duration were explained by the stimulation of the activity of MCE of microbial origin, by the pH level of milk before coagulation (below pH 6.4). It was shown that modern MCEs of microbial origin could be recommended as a cost-effective replacement for more expensive rennet and recombinant chymosins in the production of soft and fresh cheeses.

Background. Prediction of the new coronavirus infection (COVID-19) spread is important to take timely measures and initiate systemic preventive and anti-epidemic actions both at the regional and state levels to reduce morbidity and mortality.Objective: to develop a model for short-term forecasting of COVID-19 cases and deaths in the Russian Federation.Material and methods. The data for the model training were collected from the Stopcoronavirus.rf and Johns Hopkins University portals. It included 13 features to assess the infection dynamics and mortality, as well as the rate of morbidity and mortality in different countries and certain regions of the Russian Federation. The model was trained by the CatBoost gradient boosting method and retrained daily with updated data.Results. The forecast model of COVID-19 cases and deaths for the period of up to 14 days was created. The mean absolute percentage error (MAPE) estimate of the model’s accuracy ranged from 2.3% to 24% for 85 regions of the Russian Federation. The advantage of the CatBoost machine learning method over linear regression was shown using the example of the root mean square error (RMSE) value. The model showed less error for regions with a large population than for less populated ones.Conclusion. The model can be used not only to predict the pandemic of the novel coronavirus infection but also to control and assess the spread of diseases from the group of new infections at their emergence, peak incidence, and stabilization period.

Abstract Introgressive hybridization can pose a serious threat to endangered species which have an overlapping distribution such as in the case of two polecat species, Mustela eversmanii and M. putorius, in Europe. The population size of steppe polecat is known to continuously shrink, whereas its sister species, the European polecat, is still somehow widespread. In this study, we perform an analysis using microsatellite (SSR) and genomic (SNP) data sets to identify natural hybrids between polecats. Four populations were genotyped for eight polymorphic SSR loci, and thousands of unlinked SNPs were generated using a reduced‐representation sequencing approach, RADseq, to characterize the genetic make‐up of allopatric populations and to identify hybrids in the sympatric area. We applied standard population genetic analyses to characterize the populations based on their SSR allelic frequency. Only a single sample out of 48 sympatric samples showed exact intermediacy that we identified as an F1 hybrid. Additionally, one specimen was indicated in the genomic data sets as backcrossed. Other backcrosses, indicated by SSRs, were not validated by SNPs, which highlights the higher efficacy of the genomic method to identify backcrossed individuals. The low frequency of hybridization suggests that the difference in habitat preference of the two species may act as a barrier to admixture. Therefore, it is apparently unlikely that polecat populations are threatened by significant introgression. The two species showed a clear genetic differentiation using both techniques. We found higher genetic diversity values in the sympatric steppe polecat population than in the other studies on polecat populations. Although M. putorius is a hunted species in most countries, genetic diversity values indicate worse conditions in Europe than in the protected sibling species M. eversmanii. Suspending hunting and providing protected status of the former seems to be reasonable and timely.

The aim of the study is to investigate activity of markers of oxidative and nitrosative stresses in blood plasma of patients in the I–II stages of Parkinson’s disease (PD) and to determine correlations between their concentrations and severity of non-motor PD symptoms. Materials and methods. 67 patients at I–II PD stages and 20 healthy controls took part in the research. Cognitive functions were examined due to the Montreal Cognitive Assessment test – MoCA test. For the severity of psycho-emotional disorders evaluation the following scales and questionnaires were used: Night Sleep Assessment Questionnaire by A. M. Vein, Zung test for anxiety, apathy Starkstein scale, Boston stress-resistance test, Beck Depression Inventory (BDI-II). We performed ELISA test for determination of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities and 3-nitrotyrosine (3-NT) level in blood plasma of participants (Elabscience® kit). Results. The middle age of PD patients and healthy controls was 64.35 ± 1.22 and 66.40 ± 0.70 years, respectively. GPx activity in plasma of patients at І–ІІ PD stages was significantly lower than in healthy controls (P

This is the review of the book dedicated to the 110 birth anniversary of the outstanding philologist Boris I. Purishev (1903-1989). He was not only a researcher - Germanist and medievalist, but a Teacher first of all. For 60 years he worked at the first educational institution in Russia – Moscow State Pedagogical University and has educated and trained the whole pleiad of philologists, researchers and higher school pedagogues.

The study presents a new approach to the identification of various online social networks’ users that allows for matching of accounts belonging to the same person. To achieve this goal, images extracted from digital footprints of users are used. The proposed new approach compares not only the main images of a user’s profile, but also all the elements of the graphic content published in a user’s account. The described approach requires a pairwise comparison of the images published by users in two accounts from different online social networks on the “all-to-all” principle to assess the probability that these accounts belong to the same user. The comparison of the labeled graphical content elements is performed using the well-known perceptual hash method called pHash. A computational experiment was conducted to evaluate the results obtained by using the proposed approach, the f1-score achieved 0.886 for three matched images. It is shown that the results of the pHash image comparison can be used for account identification as a standalone approach as well as to complement other identification approaches. The proposed algorithm can be used to supplement the existing methods for comparative analysis of accounts. Automation of the proposed approach provides a tool for aggregation and makes it possible to obtain more information about users, assessing the depth of their personality features. The results can be applied to forming a digital twin of the user for further description of his (or her) traits in the tasks of protection against social engineering attacks, targeted advertising, assessment of creditworthiness, and other studies related to online social networks and social sciences.

The spectrophotometric method for measuring protein content can be used to evaluate the degree of proteolysis in cheeses. At a wavelength of 280 nm, tryptophan and tyrosine are absorbed, a high amount of them is found in casein, the main protein of cheese mass. It was found that the value of the absorbance coefficient of the solution of proteins extracted from flavoring additives with cheese flavor (FA) and cheeses depends on the degree of proteolysis of proteins in the cheese mass and differs in FA and different types of cheeses. The highest absorbance coefficient is observed in the FA samples A1%1cm = 10.30, in which from 65 to 81% of the protein is converted into a soluble state. In cheeses, the degree of proteolysis is from 23 to 33%, and the absorbance coefficient of solution is from 1.1 to 2.4 (with the exception of Cheddar cheese), which indicates an incomplete transition of amino acids absorbing radiation at 280 nm into the extract released from cheeses. Using the spectrophotometric method, the results of measuring the content of soluble protein in cheeses and FA, strictly correlating with the results achieved by the Kjeldahl method (R2 > 0.81), can be obtained. To get reliable results of evaluating the content of water-soluble protein in cheeses, it is necessary to carry out measurements on a sample of cheeses belonging to the same species group, having the same specificity of proteolysis and slightly different absorbance coefficient between samples within the instance.

Turbulence in fluids is an ubiquitous phenomenon, characterized by spontaneous transition of a smooth, laminar flow to rapidly changing, chaotic dynamics. In 1883, Reynolds experimentally demonstrated that, in an initially laminar flow of water, turbulent motions emerge without any measurable external disturbance. To this day, turbulence remains a major unresolved phenomenon in fluid mechanics; in particular, there is a lack of a mathematical model where turbulent dynamics emerge naturally from a laminar flow. Recently, we proposed a new theory of turbulence in gases, according to which turbulent motions are created in an inertial gas flow by the mean field effect of the intermolecular potential. In the current work, we investigate the effect of viscosity in our turbulence model by numerically simulating the air flow at normal conditions in a straight pipe for different values of the Reynolds number. We find that the transition between laminar and turbulent flow in our model occurs, without any deliberate perturbations, as the Reynolds number increases from 2000 to 4000. As the simulated flow becomes turbulent, the decay rate of the time averaged Fourier spectrum of the kinetic energy in our model approaches Kolmogorov’s inverse five-thirds law. Both results are consistent with experiments and observations.

The study combines available data on species distribution in eastern Indochina to investigate the phylogeographical genetic and morphological diversity of tree rats (Chiromyscus, Rodentia, Muridae) and to specify their natural ranges. We examined the diversity and distribution of tree rats over its range, based on recent molecular data for mitochondrial (Cyt b, COI) and nuclear (IRBP, RAG1 and GHR) genes. The study presents the most complete and up-to-date data on the distribution and phylogeography of the genus in eastern Indochina. As revealed by mitochondrial genes, C. langbianis splits into at least four coherent geographically-distributed clades, whereas C. thomasi and C. chiropus form two distinctive mitochondrial clades each. Chiromyscus langbianis and C. chiropus show significant inconsistency in nuclear genes, whereas C. thomasi shows the same segregation pattern as can be traced by mitochondrial markers. The Northern and Southern phylogroups of C. thomasi appear to be distributed sympatrically with northern phylogroups of C. langbianis in most parts of eastern Indochina. The mitochondrial clades discovered are geographically subdivided and divergent enough to suspect independent subspecies within C. langbianis and C. thomasi. However, due to the insufficiency of obvious morphological traits, a formal description is not carried out here. The processes of recent fauna formation, species distribution patterns, dispersion routes and possible natural history in Indochina are discussed.

The method based on the determination of the amount of active amino groups using o-Phthaldialdehyde (OPA method) can be applied in practice to assess accurately the degree of proteolysis in cheeses. The work establishes that the OPA method gives results that strictly correlate (R2 > 0.80, p < 0.01) with the results of assessing the degree of proteolysis by the Kjeldahl method. The results of the OPA method, expressed in the absorption intensity of the colored sample at a wavelength of 340 nm (OD340), can be converted to the content of soluble nitrogenous substances in cheese (WSN), using the calibration relationship between these indicators.The accuracy of the calibration relationship between WSN and OD340 can be increased (R2 > 0.91, p< 0.01) when using the OPA method in relation to a homogeneous group of cheeses produced by the same technology using the same type of milk clotting enzyme and lactic acid starter culture and having a similar shape of the molecular mass distribution of proteolysis products.The OPA method can be used to assess the content of proteolysis products, which form cheese flavor, in EMС. The results of assessing the degree of proteolysis by the OPA method (OD340) are proportional to both the total content of soluble nitrogen and the proportion of nitrogenous substances in it with a mass of less than 0.5 kDa, which make the greatest contribution to the formation of cheese flavor.The advantage of using the OPA method for assessing proteolysis in cheeses and EMC instead of the Kjeldahl method is a simpler measurement procedure and the possibility of studying more samples in less time.

The emergence of new factors affecting the dynamics of the number of branches of national banks, stricter financial policies of national economies aimed at controlling the performance of financing operations by transnational financial institutions, increasing the range of services provided by transnational banks are currently contributing to a change in the strategy for realizing the economic interests of transnational banks. It is economically inexpedient to compete with transnational banks of developed economies for banks in developing countries. The options for promoting their services and gaining market share can be the search for a certain category of customers who can’t afford the services of international banks, as well as the conclusion of transactions for joint project financing of the largest national industrial and infrastructure projects. In this paper, we study the process of transnationalization of bank capital in the face of changes in the institutional environment of countries - recipients of capital.

The effect of the type and dose of milk-clotting enzymes (Chy-max® M based on recombinant camel chymosin, Fromase® TL based on Rhizomucor miehei protease) on the physicochemical, functional properties and shelf life of pizza-cheeses was studied. When using a low dose of milk-clotting enzymes (MCE) for milk coagulation (250–1100 IMCU per 100 kg of milk), cheeses were obtained with an increased moisture content (55–57%), excessive acidity (pH 4.8–4.9) and texture defects (incoherent, crumbly, with separation of free moisture). This is due to the formation of a weak curd, which releases moisture poorly during processing. The use of an increased dose of MCE makes it possible to obtain a denser curd, better releasing moisture. Cheese produced with a high dose of milk-clotting enzymes (2000–2800 IMCU per 100 kg of milk) had a lower moisture content (52–53%) and lower acidity (pH 5.0–5.1). The protein matrix is more hydrated in these cheeses, which ensures its better water holding capacity and a more homogeneous and cohesive texture. The use of an increased dose of MCE with a high total proteolytic activity (Fromase) gives undesirable consequences in the form of accelerated proteolysis of cheese mass proteins, rapid loss of functional properties of the cheese, and a decrease in the shelf life of cheese (less than 60 days). Cheese production using an increased dose of MCE with a low level of total proteolytic activity (Chy-max M) allows achieving a low level of proteolysis during cheese ripening and increasing its shelf life.

In the published paper [1], we used the spatial correlation function R(σ) of two spheres, each of diameter σ, to construct a closure to the BBGKY hierarchy of hard spheres [...]