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Made available in DSpace on 2022-05-01T09:31:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Objectives: This study evaluated the effect of deep margin elevation (DME) and restorative materials (leucite-reinforced glass–ceramics [C] vs. indirect resin composite [R]) on the fatigue behavior and stress distribution of maxillary molars with 2-mm deep proximal margins restored with MOD inlay. Methods: Fifty-two extracted human third molars were randomly assigned into four groups (n = 13): C; DME + C; R; and DME + R. Inlays were fabricated in CAD-CAM and bonded to all teeth. The fatigue behavior was assessed with the stepwise stress test (10,000 cycles/step; step = 50 N; 20 Hz; initial load = 200 N). Fatigue failure loads and the number of cycles were analyzed with 2-way ANOVA and Tukey’s test (p < 0.05) and Kaplan–Meier survival plots. The stress distribution was assessed with finite element analysis. The models were considered isotropic, linear, and homogeneous, and presented bonded contacts. A tripod axial load (400 N) was applied to the occlusal surface. The stress distribution was analyzed with the maximum principal stress criterion. Results: For fatigue, there was no difference for DME factor (p > 0.05). For the material factor, the load and number of cycles for failure were statistically higher in the R groups (p < 0.05). The finite element analysis showed that resin composite inlays concentrated more stress in the tooth structure, while ceramic inlays concentrated more stress in the restoration. Non-reparable failures were more frequent in the resin composite inlays groups. Conclusions: DME was not negative for fatigue and biomechanical behaviors. Resin composite inlays were more resistant to the fatigue test, although the failure mode was more aggressive. Clinical significance: DME does not impair mechanical behavior. Resin composite inlays failed at higher loads but with a more aggressive failure mode. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp), 777th Eng. Francisco José Longo Av., SP Post-Graduate Program in Dentistry University of Taubaté (UNITAU), 09th Rua Dos Operários St., SP Graduate Program of Oral Science Prosthodontics Unit School of Dentistry Federal University of Santa Maria (UFSM) Roraima Av, RS School of Dentistry São Francisco University (USF), 218th São Francisco de Assis Av., SP Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp), 777th Eng. Francisco José Longo Av., SP Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp), 777th Eng. Francisco José Longo Av., SP Department of Restorative Dentistry Institute of Science and Technology São Paulo State University (Unesp), 777th Eng. Francisco José Longo Av., SP

Made available in DSpace on 2022-05-01T09:30:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 This study aimed to evaluate the biomechanical behavior, stress distributions and bone microstrain of fixed partial dentures (FPD) with ceramic abutments supported on monotype zirconia implants, titanium implants and two-piece zirconia implants, using finite element analysis. A three-dimensional model of the jaw was simulated containing 1.0 mm thick cortical bone and cancellous bone tissue. A FPD and implant models (4.1 x 10 mm) were modeled containing a cement-retained implant abutment. These models were replicated in three groups with similar geometries: Titanium Implant and Zirconia Abutment (Ti-Zr); Zirconia Implant and Zirconia Abutment (Zr-Zr) and Monotype Zirconia Implant (Zr-S). An axial load of 300 N was applied to the center of the first premolar. The microstrain (με) and the Von-Mises stress (MPa) were assumed as failures criteria. For the three groups, a higher stress concentration was observed in the region of FPD connectors. The Ti-Zr group showed a higher stress concentration in the prosthesis and implant when compared to the other groups. However, the smaller elastic modulus of the titanium implant, in relation to the zirconia, provided a lower stress in the abutment and in the prosthetic screw. The monotype implant system allowed a more homogeneous stress distribution and its strain were predominantly located in the cervical region of the peri-implant bone tissue. Monotype or two-piece zirconia implants can be used for rehabilitation with FPD. However, the absence of separation between implant and abutment in the monotype system avoids the stress concentration in the prosthetic screw and reduced the peri-implant bone strain. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNESP) Department of Dentistry Universidade São Francisco (USF) Department of Implantology College of Denstristry CECAPE Juazeiro do Norte Department of Dentistry University of Taubaté (UNITAU) Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNESP)

Made available in DSpace on 2022-04-29T08:40:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Objective: The aim of this study was to investigate the effect of the hybrid abutment with different angles (0 and 15 degrees) on the stress distribution and deformation on maxillary implant-supported fixed prosthesis, using digital image correlation (DIC) and finite element analysis (FEA). Materials and Methods: For DIC, two situations were considered: conventional straight implant placement and implant placement with 15 degrees inclination. Different zirconia mesostructures were milled, one straight and the other with a 15-degree angulation to correct the implant positioning. Then, the zirconia mesostructures were cemented to the titanium base (Ti base), and both groups received a lithium disilicate crown. The DIC technique was performed to measure the deformation generated on the simulated bone surface (150 N loading). For the FEA (in silico), three-dimensional numerical models based on the in vitro setup were modeled using computer-aided design software. All materials were considered elastic, isotropic, and homogeneous. Comparison of both methods showed coherence between the in vitro and in silico results. The von-Mises stress of the implants, Ti base and screw, and the maximum principal stress in the mesostructure and crown were calculated for both conditions. Results: The overall surface deformation distributions determined by both techniques were considered similar allowing the model validation. The higher deformation was found in the cervical region with a higher magnitude for the angled hybrid abutment. The same pattern was observed in the stress fields regardless of the analyzed region and structure. Conclusion: Based on this study, using an angled hybrid abutment to correct the implant positioning generated higher stress in the implant fixture, surrounding tissue, Ti base, screw, and crown. Therefore, the implant should be positioned axially, whenever possible, to reduce the mechanical complications. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNSEP) São José Dos Campos Department of Dentistry University of Taubaté (UNITAU), Taubaté Department of Dental Materials Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and Vrije Universiteit Amsterdam Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNSEP) São José Dos Campos

Made available in DSpace on 2022-04-28T19:43:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Background: Mesenchymal cells’ biology has been an important investigative tool to maximize bone regeneration through tissue engineering. Here we used mesenchymal cells from periodontal ligament (PDLCs) with high (h-) and low (l-) osteogenic potential, isolated from different donors, to investigate the impact of the individual epigenetic and transcriptional profiles on the osteogenic potential. Methods: Genome-wide and gene-specific DNA (hydroxy) methylation, mRNA expression and immunofluorescence analysis were carried out in h- and l-PDLCs at DMEM (non-induced to osteogenesis) and OM (induced—3rd and 10th days of osteogenic differentiation) groups in vitro. Results: Genome-wide results showed distinct epigenetic profile among PDLCs with most of the differences on 10th day of OM; DMEMs showed higher concentrations (xOM) of differentially methylated probes in gene body, intronic and open sea (3rd day), increasing this concentration in TSS200 and island regions, at 10 days. At basal levels, h- and l-PDLCs showed different transcriptional profiles; l-PDLCs demonstrated higher levels of NANOG/OCT4/SOX2, BAPX1, DNMT3A, TET1/3, and lower levels of RUNX2 transcripts, confirmed by NANOG/OCT4 and RUNX2 immunofluorescence. After osteogenic induction, the distinct transcriptional profile of multipotentiality genes was maintained among PDLCs. In l-PDLCs, the anti-correlation between DNA methylation and gene expression in RUNX2 and NANOG indicates methylation could play a role in modulating both transcripts. Conclusions: Epigenetic and transcriptional distinct profiles detected at basal levels among PDLCs were maintained after osteogenic induction. We cannot discard the existence of a complex that represses osteogenesis, suggesting the individual donors’ characteristics have significant impact on the osteogenic phenotype acquisition. School of Dentistry Health Science Institute Paulista University Department of Prosthodontics and Periodontics Piracicaba Dental School University of Campinas Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University Department of Dentistry University of Taubaté Program in Environmental and Experimental Pathology Paulista University Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University

Made available in DSpace on 2021-06-25T11:13:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 The aim of the present study was to investigate the effect of shrinking and no shrinking dental filling materials combination in posterior restorations under the combined effects of polymerization shrinkage and occlusal load by means of 3D Finite Elements Analysis. Six computer-generated and restored class I or class II cavities models of a lower molar were designed in the CAD software and evaluated according to the cavity and restorative procedure. Different shrinking and no shrinking adhesive materials combination with diverse Young’s modulus were considered. A food bolus was modeled on the occlusal surface replicating the chewing load using static linear analyses Polymerization shrinkage was simulated for the shrinking different restorative materials. The maximum principal stress was selected as analysis criteria. All models exhibited higher stresses along the dentine restoration interfaces with different magnitude and a similar stress trend along enamel restoration interface. Stress values up to 22 MPa and 19 MPa were recorded in the enamel and restoration, respectively. The use of elastic not shrinking material layer in combination with bulk fill composite reduced the stress magnitude in dentine and enamel to replace dental tissues. Class I and class II posterior cavities adhesively restored with shrinking filling material’s combination showed the most unfavorable stress concentrations and the multilayer technique is a promising restorative alternative in posterior adhesive restorations when deep dentin and enamel volumes are missing. School of Dentistry University of Naples Federico II, via S. Pansini 5 Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University-UNESP Fraunhofer JL IDEAS Department of Industrial Engineering University of Naples Federico II Endodontic Clinical Section School of Dentistry Department of Biomedical and Neuromotor Sciences University of Bologna-Alma Mater Studiorum School of Dentistry University of Taubaté–UNITAU, Taubaté Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University-UNESP

Aim: To investigate the effect of toothbrushing on different stained Computerd Aided Design/Computer Aided Manufacturing CAD/CAM ceramics. Materials and Methods: Fifty specimens (high translucency zirconia, YZHT, zirconia-reinforced lithium silicate, ZLS, feldspathic, FDL, hybrid ceramic, HC, all from Vita Zahnfabrik) were divided into five groups according to their staining technique. ZLS allowed the composition of two groups: ZLS1 with crystallization and staining together, ZLS2, with the stain firing after the crystallization. YZHT received a stain firing after the sintering process, and FDL received stain firing directly. The HC was stained with acrylic staining, and was light-cured on its surface. The specimens were brushed in total for 150,000 cycles at 2.45 N with 180 strokes/min. Surface measurements to obtain Rz were performed after 50,000, 100,000 and 150,000 cycles, with five evaluation lines (5 mm) per specimen orthogonal to the brushing direction, covering brushed and unbrushed areas. The wear was analyzed using two-way ANOVA and Tukey tests (α = 5%). Scanning electron microscopy (SEM) was performed to access the surface profile. The wear was affected by material (p &lt, 0.001) and time (p = 0.139). Superior wear (µm) was observed for HC (6.6 ± 4.4, 6.8 ± 4.0, 9.2 ± 3.5) compared to ZLS2 (1.0 ± 0.3, 1.2 ± 2.2, 1.3 ± 0.3), YZHT (1.0 ± 0.1, 1.2 ± 0.3, 1.2 ± 0.3), ZLS1 (0.9 ± 0.1, 1.1 ± 0.5, 1.2 ± 0.3) and FDL (0.9 ± 0.1, 0.9 ± 0.1, 1.0 ± 0.2) after 5, 10 and 15 years of simulation, respectively. SEM showed different wear patters for HC with the removal of the glaze layer. HC showed a higher staining wear rate compared to the glass-based and polycrystalline ceramics after 15 years. The extrinsic characterization of feldspathic ceramic showed its superior longevity compared to the evaluated high-translucency zirconia, zirconia-reinforced lithium silicate, and hybrid ceramic.

Made available in DSpace on 2021-06-25T11:10:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 This study evaluated the polymerization shrinkage stress of three tooth preparation designs for indirect ceramic overlay by finite element analysis: isthmus preparation (IST); without isthmus preparation (wIST); and non-retentive preparation (nRET). The models were created based in prepared dental typodonts and were digitally impressed with an intraoral scanner. The interfaces in all models were considered perfectly bonded and all materials were considered homogeneous, linear, and isotropic. The polymerization shrinkage of the cement layer (100 µm) was simulated and evaluated by maximum principal stress criteria. The stress peaks followed this sequence: restoration = IST (13.4 MPa) > wIST (9.3 MPa) > nRET (9 MPa); cement layer = IST (16.9 MPa) > wIST (12.6 MPa) > nRET (10-7.5 MPa); and teeth = IST (10.7 MPa) > wIST (10.5 MPa) > (9 MPa). For the cement layer, the non-retentive preparation (nRET) had the lowest shrinkage stress from all the groups, obtaining a more homogeneous stress distribution on the cement surface. Regarding the abutment teeth, the IST generated a higher shrinkage stress area on the dental structure, concentrating higher stress magnitude at the axiopulpar and axiogingival angles. Non-retentive preparation seems to reduce polymerization shrinkage stress. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNESP) Departament of Dentistry Postgraduate Program in Dentistry University of Taubaté (UNITAU) Private Practice Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNESP)

Made available in DSpace on 2020-12-12T02:35:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: To evaluate the composition, flexural strength and fatigue behaviour of lithium disilicate ceramic (LD) after repeated firings and different staining techniques. Methods: LD discs were fabricated and divided according to number of firing cycles and staining technique: CO - control, discs were crystallized (850 °C/10 min); SC – single-step characterization – crystallization and staining (applied with a thin brush) were performed in a single step with one firing cycle (850 °C/10 min); and DC – double-step characterization – crystallization firing cycle was performed first (850 °C/10 min), followed by staining firing cycle (770 °C/90 s). Specimens were fired two, four or six times (one crystallization firing cycle and one, three or five staining firing cycles), resulting into 9 groups (n = 30): COII, COIV, COVI, SCII, SCIV, SCVI, DCII, DCIV and DCVI. The composition of the specimens was investigated (EDS, XRD, Raman spectroscopy), and the biaxial flexural strength (n = 10) and staircase tests (n = 20, 5 × 104 cycles, 5 Hz) were performed. Data were subjected to one-way ANOVA and Tukey's test (α = 0.05). Results: EDS and XRD revealed amorphous content for stained groups. Biaxial flexural strength was not affected by repeated firings in any group, but stained groups presented lower flexural strength than control groups (p = 0.001). The fatigue limit results decreased in all groups compared to flexural strength. SC groups showed similar (SCII and SCIV) or even higher fatigue limits (SCVI) than the control groups, and DC showed the lowest fatigue limit values. SEM and Raman suggested that the interfaces between staining and the LD showed only an overlap for the DC groups, whereas for the SC it was suggested an interaction between the stain and the LD. Significance: Repeated firings did not result in decreased lithium disilicate flexural strength.Staining affected flexural strength and also resulted in increased amorphous content in the characterized specimens. Single-step staining resulted in the highest fatigue limit. Institute of Science and Technology UNESP – Paulista State University São José dos Campos Technological Institute of Aeronautics São José dos Campos Dental School University of São Paulo Department of Dentistry University of Taubaté Institute of Science and Technology UNESP – Paulista State University São José dos Campos FAPESP: 2017-11913-8

Made available in DSpace on 2022-04-29T08:39:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Objective The aim of this study was to evaluate, by means of a fatigue life test, different ceramic materials used in palatal veneers to restore the canine guidance. Materials and Methods Forty-five standardized anatomical preparations were made in extracted healthy human canines with 1.2 uniform thickness. Samples were scanned, restorations were designed and milled in polymer-infiltrated ceramic network (PICN, Vita Enamic), zirconia-reinforced lithium silicate (ZLS, Vita Suprinity), and high translucent yttrium oxide-stabilized tetragonal zirconia (YZHT, Vita YZHT). Dental preparations were etched, restorations were processed according to the manufacturers' recommendations, and adhesively cemented. Then, three samples of each group were tested with load-to-fracture to determine the fatigue parameters. In addition, the palatal veneers stresses were evaluated using numerical models through finite element analysis. Results The mean of the monotonic test for PICN, ZLS, and YZHT was 674.18 N, 560.5 N, and 918.98 N, respectively. The StepWise test was performed until specimen fracture or until suspension of the test after 1.2 × 10 6cycles. Regarding survival, using the Kaplan-Meier method, PICN presented results for the mean and median of 245.21 N and 225 N, respectively; ZLS had an average of 175.76 N and a median of 168 N, and YZHT with an average of 383.30 N and a median of 366 N. Regarding the Weibull method, PICN showed results of 5.43 β and 264 η for form and scale, respectively; ZLS had 36.14 β for form and 380.67 η for scale; and YZHT presented 4.95 β for form and 417.38 η for scale. The highest stress value was calculated for YZHT, ZLS, and PICN, respectively. Conclusions It was possible to conclude that all tested materials have the possibility of being used for rehabilitation of upper canines' palatal surface. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University, Avenue, Engenheiro Francisco Jose Longo, 777, São José dos Campos Department of Dentistry University of Taubaté, Taubaté Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University, Avenue, Engenheiro Francisco Jose Longo, 777, São José dos Campos

Made available in DSpace on 2022-04-29T08:32:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The requirement to achieve natural looking restorations is one of the most challenging aspects in dentistry. Although zirconia has provided new opportunities for achieving superior aesthetics and physicochemical outcomes, very little has been achieved for its cellular and molecular performance, especially considering angiogenesis and osteogenesis. As angiogenesis is a secondary event and concomitant to osteogenesis, an indirect effect of dental implant on endothelial cells could be the release of active molecules such as those already reported affecting osteoblasts. To better address this issue, we challenged human endothelial cells (HUVECs) with zirconia-conditioned medium up to 72 h to allow analysis specific gene expression and protein pattern of mediators of epigenetic machinery in full. Our data shows involvement of zirconia in triggering intracellular signaling through MAPK-ERK activation, leading the signal to activate histone deacetylase HDAC6 likely with concomitant well-modulated DNA methylation profile by DNMTs and TETs. These signaling pathways seem to culminate in cytoskeleton rearrangement of endothelial cells, an important prerequisite to cell migration expected in angiogenesis. Collectively, this study demonstrates for the first time epigenetic-related molecular mechanism involved in endothelial cells responding to zirconia, revealing a repertoire of signaling molecules capable of executing the reprogramming process of gene expression, which are necessary to drive cell proliferation, migration, and consequently angiogenesis. This set of data can further studies using gene editing approaches to better elucidate functional roles. Lab. of Bioassays and Cellular Dynamics Department of Chemistry and Biochemistry Institute of Biosciences UNESP – São Paulo State University Department of Dentistry University of Taubaté Program in Environmental and Experimental Pathology Paulista University Lab. of Bioassays and Cellular Dynamics Department of Chemistry and Biochemistry Institute of Biosciences UNESP – São Paulo State University

Made available in DSpace on 2022-05-01T09:47:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-02 The aim of this study was to evaluate the effect of biologically oriented preparation technique on the stress concentration of endodontically treated upper central incisors restored with zirconia crown (yttria-stabilized zirconia polycrystalline ceramic) through finite element analysis (FEA). Four models of maxillary central incisors containing enamel, dentin, periodontal ligament, cortical and medullary bone were created in CAD. Each model received a polymeric core-build up with nanofilled dental resin composite. The evaluated models were SM—preparation in shoulder 90◦; CM—chamfer preparation; BOPT—biologically oriented preparation technique and BOPTB—BOPT preparation 1 mm below the cement-enamel junction. All models received zirconia crowns (5Y-TZP), fiberglass post and 1 mm ferrule. The models were imported into the analysis software with parameters for mechanical structural testing using the maximum principal stress and the tensile strength as the analysis criteria. Then, load of 150 N was applied at the cingulum with 45◦ slope to the long axis of the tooth, with the fixed base for each model. The type of marginal preparation affected the stresses concentration in endodontically treated teeth and in the zirconia crown margin. Considering the stress magnitude only, BOPT is a viable option for anterior monolithic zirconia crowns; however, with the highest stress magnitude at the restoration margin. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp) Faculty of Dentistry Universidade Estadual do Oeste do Paraná (UNIOESTE) Department of Dentistry University of Taubaté (UNITAU) School of Dentistry University of Naples Federico II, via S. Pansini 5 Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp)

Made available in DSpace on 2022-05-01T12:56:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 PURPOSE. To characterize the microstructure of three yttria partially stabilized zirconia ceramics and to compare their hardness, indentation fracture resistance (IFR), biaxial flexural strength (BFS), and fatigue flexural strength. MATERIALS AND METHODS. Disc-shaped specimens were obtained from 3Y-TZP (Vita YZ HT), 4Y-PSZ (Vita YZ ST) and 5Y-PSZ (Vita YZ XT), following the ISO 6872/2015 guidelines for BFS testing (final dimensions of 12 mm in diameter, 0.7 and 1.2 ± 0.1 mm in thicknesses). Energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses were performed, and mechanical properties were assessed by Vickers hardness, IFR, quasi-static BFS and fatigue tests. RESULTS. All ceramics showed similar chemical compositions, but mainly differed in the amount of yttria, which was higher as the amount of cubic phase in the diffractogram (5Y-PSZ > 4Y-PSZ > 3Y-TZP). The 4Y- and 5Y-PSZ specimens showed surface defects under SEM, while 3Y-TZP exhibited greater grain uniformity on the surface. 5Y-PSZ and 3Y-TZP presented the highest hardness values, while 3Y-TZP was higher than 4Y- and 5Y-PSZ with regard to the IFR. The 5Y-PSZ specimen (0.7 and 1.2 mm) showed the worst mechanical performance (fatigue BFS and cycles until failure), while 3Y-TZP and 4Y-PSZ presented statistically similar values, higher than 5Y-PSZ for both thicknesses (0.7 and 1.2 mm). Moreover, 3Y-TZP showed the highest (1.2 mm group) and the lowest (0.7 mm group) degradation percentage, and 5Y-PSZ had higher strength degradation than 4Y-PSZ group. CONCLUSION. Despite the microstructural differences, 4Y-PSZ and 3Y-TZP had similar fatigue behavior regardless of thickness. 5Y-PSZ had the lowest mechanical performance. [J Adv Prosthodont 2021;13:385–95] Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) Institute of Science and Technology College of Dentistry University of Taubaté (Unitau) Technological Institute of Aeronautics Physics department College of Dentistry Federal University of Santa Maria (UFSM) Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) Institute of Science and Technology

Made available in DSpace on 2021-06-25T11:04:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 This in vitro study investigated whether Carbopol 980 polymer could potentiate the anti-erosive effect of solutions containing sodium fluoride (F) and sodium fluoride associated with stannous chloride (FS). The dissolution of hydroxyapatite treated with the experimental solutions (F [500 ppm F-], F + Carbopol [0.1%], FS [500 ppm F- + 800 ppm Sn2+], FS + Carbopol) was evaluated. Deionized water was the negative control, and a commercial mouth rinse (AmF/NaF/SnCl2; 500 ppm F + 800 ppm Sn2+; Elmex® Erosion Protection) was the positive control. The solutions were also evaluated in an erosion-rehardening protocol, with two treatments per day, using bovine enamel specimens (n = 15) and human saliva. The acid challenge was performed using 0.3% citric acid (pH 2.6) for 2 min. Microhardness was measured at different times: baseline, after the first erosive challenge, after treatment, and after the second erosive challenge. Based on microhardness values, the demineralization, rehardening, and protective potentials were calculated. The alkali-soluble fluoride on enamel surfaces was also measured. Data were analyzed using ANOVA and Tukey tests (α = 0.05). Groups treated with FS + Carbopol showed the lowest hydroxyapatite dissolution and the highest rehardening and protective potentials. The measurement of alkali-soluble fluoride on enamel surfaces was also higher in the FS + Carbopol group. Carbopol was able to significantly increase the protective effect of the fluoridated solutions in addition to optimizing the adsorption of fluoride on the enamel surface. Department of Restorative Dentistry Institute of Science and Technology São Paulo State University - UNESP Avenida Engenheiro Francisco José Longo, 777, Jardim São Dimas Department of Restorative Dentistry Dental School University of Taubaté Department of Restorative Dentistry São Paulo University - USP Department of Chemical Engineering Polytechnic School São Paulo University - USP Department of Restorative Dentistry Institute of Science and Technology São Paulo State University - UNESP Avenida Engenheiro Francisco José Longo, 777, Jardim São Dimas

Made available in DSpace on 2020-12-12T01:50:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Objective: Evaluate the effect of staining and repeated firings on color, translucency and surface proprieties of CAD/CAM lithium disilicate (LD). Material and Methods: One hundred eighty LD discs were made (ISO 6872) and distributed in different groups (n = 20): control (CO)—no treatment; single-step characterization (SC)—crystallization and staining fired together; and double step characterization (DC)—crystallization and then staining. The samples were submitted to two, four, or six firings, resulting in nine groups: COII, COIV, COVI, SCII, SCIV, SCVI, DCII, DCIV, and DCVI. The color and translucency were measured by a reflectance spectrophotometer. Surface roughness (Ra) and Vickers nano-hardness were also measured. ANOVA and Tukey statistical tests were used (α =.05). Results: Only CO and DC demonstrated significant color alterations (ΔE00 > 1.8). SC and DC did not show changes in translucency by the number of firings (P >.05); however, for CO (P =.02) these values increased. Nano-hardness was similar in all groups (P >.05). Ra values indicated differences due to the type of characterization (SC presented the highest values) and number of firings (CO and DC groups) (P

Made available in DSpace on 2020-12-12T01:08:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Modifications on shear stress-based mechanical forces are associated with pathophysiological susceptibility and their effect on endothelial cells (EC) needs to be better addressed looking for comprehending the cellular and molecular mechanisms. This prompted us to better evaluate the effects of shear stress in human primary venous EC obtained from the umbilical cord, using an in vitro model to mimic the laminar blood flow, reaching an intensity 1–4 Pa. First, our data shows there is a significant up-expression of phosphatidylinositol 3-kinase (PI3K) in shear-stressed cells culminating downstream with an up-phosphorylation of AKT and up-expression of MAPK-ERK, concomitant to a dynamic cytoskeleton rearrangement upon integrin subunits (α4 and ß 3) requirements. Importantly, the results show there is significant involvement of nitric oxide synthase (eNOS), nNOS, and vascular endothelial growth factors receptor 2 (VEGFR2) in shear-stressed EC, while cell cycle-related events seem to being changed. Additionally, although diminution of 5-hydroxymethylcytosine in shear-stressed EC, suggesting a global repression of genes transcription, the promoters of PI3K and eNOS genes were significantly hydroxymethylated corroborating with their respective transcriptional profiles. Finally, to better address, the pivotal role of PI3K in shear-stressed EC we have revisited these biological issues by wortmannin targeting PI3K signaling and the data shows a dependency of PI3K signaling in controlling the expression of VGFR1, VGFR2, VEGF, and eNOS, once these genes were significantly suppressed in the presence of the inhibitor, as well as transcripts from Ki67 and CDK2 genes. Finally, our data still shows a coupling between PI3K and the epigenetic landscape of shear-stressed cells, once wortmannin promotes a significant suppression of ten-11 translocation 1 (TET1), TET2, and TET3 genes, evidencing that PI3K signaling is a necessary upstream pathway to modulate TET-related genes. In this study we determined the major mechanotransduction pathway by which blood flow driven shear stress activates PI3K which plays a pivotal role on guaranteeing endothelial cell phenotype and vascular homeostasis, opening novel perspectives to understand the molecular basis of pathophysiological disorders related with the vascular system. Bioassays and Cell Dynamics Laboratory Department of Chemistry and Biochemistry Bioscience Institute UNESP Department of Biology Dental School University of Taubaté Bioassays and Cell Dynamics Laboratory Department of Chemistry and Biochemistry Bioscience Institute UNESP FAPESP: 2014/22689-3 FAPESP: 2016/22270-8

Made available in DSpace on 2022-05-01T06:31:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Objective: Evaluate the effect of four preparation designs, two ceramic materials, and two occlusion contact types on the stress distribution of ceramic veneer in upper central incisor. Material and methods: 3D-models were performed in the modeling software containing enamel, dentin, pulp, periodontal ligament and a base of polyurethane resin. The designs were modeled and exported to the computer aided engineering software to perform the static structural analysis. For the mesh, a total of 155429 tetrahedron elements and 271683 nodes were used, after a 10% convergence test. Two materials, lithium disilicate and feldspathic ceramics, were simulated. A static load of 100 N on 45º was applied on the incisal and middle thirds of the palatal tooth region, guided by the occlusal plane. The base was constrained in all directions. The Maximum Principal Stress was the failure criteria chosen for the analysis. Results: The Finite Element Analysis showed that the most conservative designs presented less stress concentration on the ceramic veneer. However, the highest tensile stress concentrations were observed on lithium disilicate veneer with extend design, on the middle third. The type of occlusal contact presented different stress patterns among the preparation designs; the incisal contact showed higher stress concentration compared to middle third contact regardless the ceramic material. Conclusion: To perform a ceramic veneer in upper central incisor, the feldspathic ceramic presented promising results and should be recommended when the extended design was done. Regarding contact types, the incisal contact is more prone to failure regardless the ceramic and preparation design. São Paulo State University (UNESP) Institute of Science and Technology Department of Restorative Dentistry University of Taubaté (UNITAU) Department Dentistry São Paulo State University (UNESP) Institute of Science and Technology Department of Restorative Dentistry

Made available in DSpace on 2022-04-29T08:31:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-08-01 The loss of dental structure caused by endodontic treatment is responsible for a decrease in tooth resistance, which increases susceptibility to fracture. Therefore, it is important that minimally invasive treatments be performed to preserve the dental structure and increase the resistance to fracture of endodontically treated posterior teeth. To evaluate under axial loads, using the finite element method, the stress distribution in endodontically treated molars restored with both transfixed or vertical glass fiber posts (GFP) and resin composite. An endodontically treated molar 3D-model was analyzed using finite element analyses under four different conditions, class II resin composite (G1, control model), vertical glass fiber post (G2), transfixed glass fiber posts (G3) and vertical and transfixed glass fiber posts (G4). Ideal contacts were considered between restoration/resin composite and resin composite/tooth. An axial load (300 N) was applied to the occlusal surface. The resulting tensile stresses were calculated for the enamel and dentin tissue from five different viewports (occlusal, buccal, palatal, mesial and distal views). According to the stress maps, similar stress trends were observed, regardless of the glass fiber post treatment. In addition, for the G1 model (without GFP), a high-stress magnitude can be noticed in the proximal faces of enamel (7.7 to 14 MPa) and dentin (2.1 to 3.3 MPa) tissue. The use of transfixed glass fiber post is not indicated to reduce the stresses, under axial loads, in both enamel and dentin tissue in endodontically treated molar with a class II cavity. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp), São José dos Campos Graduate Program in Dentistry Department of Dentistry University of Taubaté (UNITAU) Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp), São José dos Campos

Made available in DSpace on 2022-04-29T08:30:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-01 Purpose: To evaluate the effect of different substrate stiffness [sound dentin (SD), resin composite core (RC) or metal core (MC)] on the stress distribution of a zirconia posterior three-unit fixed partial denture (FPD). Methods: The abutment teeth (first molar and first premolar) were modeled, containing 1.5 mm of axial reduction, and converging axial walls. A static structural analysis was performed using a finite element method and the maximum principal stress criterion to analyze the fixed partial denture (FPD) and the cement layers of both abutment teeth. The materials were considered isotropic, linear, elastic, homogeneous and with bonded contacts. An axial load (300 N) was applied to the occlusal surface of the second premolar. Results: The region of the prosthetic connectors showed the highest tensile stress magnitude in the FPD structure depending on the substrate stiffness with different core materials. The highest stress peak was observed with the use of MC (116.4 MPa) compared to RC and SD. For the cement layer, RC showed the highest values in the molar abutment (14.7 MPa) and the highest values for the premolar abutment (14.4 MPa) compared to SD (14.1 and 13.4 MPa) and MC (13.8 and 13.3 MPa). Both metal core and resin composite core produced adequate stress concentration in the zirconia fixed partial denture during the load incidence. However, more flexible substrates, such as composite cores, can increase the tensile stress magnitude on the cement. Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) Institute of Science and Technology São José dos Campos Department of Dentistry Postgraduate Program of Dentistry University of Taubaté (UNITAU) School of Dentistry University of Naples Federico II Fraunhofer Laboratory IDEAS Department of Industrial Engineering University of Naples Federico II Department of Bioscience Research College of Dentistry University of Tennessee Health Science Center The Forsyth Institute Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) Institute of Science and Technology São José dos Campos

Made available in DSpace on 2021-06-25T11:14:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Statement of problem: The extrinsic characterization with pigments and glaze application on the surface of ceramic restorations promotes individualization and esthetics. However, whether this characterization is resistant to toothbrushing abrasive wear is unclear. Purpose: The purpose of this in vitro study was to investigate the abrasive wear resistance of characterized and glazed resin-modified ceramic with different surface treatments before characterization. Material and methods: Eighty rectangular specimens (10×8×6 mm) were precision cut from Vita Enamic and randomly divided in 4 groups as per the surface treatment before the characterization technique. These groups included the following: no additional treatment or polishing only, acid etching, airborne-particle abrasion, and self-etching silanization. For each surface treatment, 2 subgroups were created considering the presence or absence of the glaze layer after shade characterization, totaling 8 groups (n=10). The specimens were submitted to toothbrushing simulation (150 000 cycles, 2.45-N, 180 strokes per minute) with a soft and straight toothbrush and a solution of toothpaste and distilled water (250 g/L). After each 50 000 cycles, the wear of the characterized surface was measured by using a contact profilometer in μm which was equivalent to 5, 10, and 15 years of simulated toothbrushing. The rates were analyzed by using 3-way ANOVA and the post hoc Tukey test (α=.05). Scanning electron microscopy analysis was performed to access the surface profile between the evaluated periods. Results: The mean wear rate (Rz) of the characterized surface was affected by the isolated factors, including surface treatment, glaze, and time (P

Made available in DSpace on 2021-06-25T10:28:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 Background: This study aimed to evaluate surface treatments, adhesives and composites for repairing silorane based restorations. Material and Methods: One hundred and twenty truncated cones (2 mm smaller diameter and 4 mm larger diameter) made of silorane composite were divided in 12 groups according with the surface treatment (diamond bur and oxide aluminum abrasion), the adhesive (Adper Scothbond Multipurpose (3M ESPE), Ecusit (Voco) and Filtek P90 Adhesive (3M ESPE)). Each group was subdivided in two according with the composite used for repair (methacrylate and silorane). The repair was made with a second truncated cone build over the first one and bond strength assssed by tensile strength. Data were submitted to ANOVA 3-way and Tukey’s test (p

Made available in DSpace on 2021-06-25T11:13:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-02 The aim of the present investigation was to calculate the stress distribution generated in the root dentine canal during mechanical rotation of five different NiTi endodontic instruments by means of a finite element analysis (FEA). Two conventional alloy NiTi instruments F360 25/04 and F6 Skytaper 25/06, in comparison to three heat treated alloys NiTI Hyflex CM 25/04, Protaper Next 25/06 and One Curve 25/06 were considered and analyzed. The instruments’ flexibility (reaction force) and geometrical features (cross section, conicity) were previously investigated. For each instrument, dentine root canals with two different elastic moduli(18 and 42 GPa) were simulated with defined apical ratios. Ten different CAD instrument models were created and their mechanical behaviors were analyzed by a 3D-FEA. Static structural analyses were performed with a non-failure condition, since a linear elastic behavior was assumed for all components. All the instruments generated a stress area concentration in correspondence to the root canal curvature at approx. 7 mm from the apex. The maximum values were found when instruments were analyzed in the highest elastic modulus dentine canal. Strain and von Mises stress patterns showed a higher concentration in the first part of curved radius of all the instruments. Conventional Ni-Ti endodontic instruments demonstrated higher stress magnitudes, regardless of the conicity of 4% and 6%, and they showed the highest von Mises stress values in sound, as well as in mineralized dentine canals. Heat-treated endodontic instruments with higher flexibility values showed a reduced stress concentration map. Hyflex CM 25/04 displayed the lowest von Mises stress values of, respectively, 35.73 and 44.30 GPa for sound and mineralized dentine. The mechanical behavior of all rotary endodontic instruments was influenced by the different elastic moduli and by the dentine canal rigidity. Endodontic Clinical Section Department of Biomedical and Neuromotor Sciences School of Dentistry University of Bologna-Alma Mater Studiorum University of Taubaté (UNITAU), Taubaté Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University—UNESP Department of Chemical Materials and Industrial Production Engineering University of Naples Federico II and the Center for Advanced Biomaterials for HealthCare@CRIB Istituto Italiano di Tecnologia School of Dentistry University of Naples Federico II Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University—UNESP

Made available in DSpace on 2021-06-25T11:13:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 The purpose of this study is to evaluate the effect of pulp chamber extension angles and filling material mechanical properties on the biomechanical response of a ceramic endocrown. A 3D model of maxillary molar that underwent endodontically treatment was exported to computer aided design software to conduct finite element analysis (FEA). The endocrown model was modified considering different pulp chamber extension angles (right angle; 6°, 12° and 18° of axial divergence). The solids were imported into the computer aided engineering software in Standard for the Exchange of Product Data (STEP) format. Nine different filling materials were simulated to seal the orifice of the root canal system under each endocrown restoration (resin composite, bulk-fill resin composite, alkasite, flowable resin composite, glass ionomer cement, autocured resin-reinforced glass ionomer cement, resin cement, bulk-fill flowable resin composite, zinc oxide cement), totaling 36 models. An axial load (300 N) was applied at the occlusal surface. Results were determined by colorimetric graphs of von-Misses stress (VMS) and Maximum Principal Stress (MPS) on tooth, cement layer, and endocrown restorations. VMS distribution showed a similar pattern between the models, with more stress at the load region for the right-angled endocrowns. The MPS showed that the endocrown intaglio surface and cement layer showed different mechanical responses with different filing materials and pulp chamber angles. The stress peaks plotted in the dispersion plot showed that the filling material stiffness is proportional to the stress magnitude in the endocrown, cement layer and tooth adhesive surface. In addition, the higher the pulp chamber preparation angle, the higher the stress peak in the restoration and tooth, and the lower the stress in the cement layer. Therefore, 6° and 12° pulp chamber angles showed more promising balance between the stresses of the adhesive interface structures. Under the conditions of this study, rigid filling materials were avoided to seal the orifice of root canal system when an endocrown restoration was planned as rehabilitation. In addition, the pulp chamber axial walls were prepared between 6° and 12° of divergence to balance the stress magnitude in the adhesive interface for this treatment modality. School of Dentistry University of Taubaté Department of Human Pathology in Adulthood and Childhood “G. Barresi” University Hospital “G. Martino” of Messina, Via Consolare Valeria 1 Institute of Science and Technology São Paulo State University (Unesp) São José dos Campos Department of Biomedical and Dental Sciences and Morphofunctional Imaging University of Messina Institute of Science and Technology São Paulo State University (Unesp) São José dos Campos

The goal of this study was to evaluate the stress distribution in an endocrown restoration according to different provisional restorative materials. An endodontically treated maxillary molar model was selected for conducting the finite element analysis (FEA), with a determined amount of dental remnant of 1.5 mm. The model was imported to the analysis software (ANSYS 19.2, ANSYS Inc., Houston, TX, USA) in STEP format. All contacts were considered perfectly bonded. The mechanical properties of each structure were considered isotropic, linear, elastic, and homogeneous. Three different provisional restorative materials were simulated (acrylic resin, bis-acrylic resin, and resin composite). An axial load (300 N) was applied at the occlusal surface in the center of the restoration. Results were determined by colorimetric stress maps of maximum principal stress, maximum shear stress, and total deformation. The different materials influenced the stress distribution for all structures, the higher the material’s elastic modulus, the lower the stress magnitude on the cement layer. In the present study, all provisional restorative materials showed similar stress patterns in the endocrown and on the cement layer however, with different magnitude. Based on this study limitation, the use of resin composite to manufacture provisional endocrowns is suggested as a promising material to reduce the stresses in the cement layer and in the dental tissue surfaces.

Made available in DSpace on 2022-04-29T08:34:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01 Objective Polymeric framework represent an innovative approach for implant-supported dental prostheses. However, the mechanical response of ultra-high performance polymers as frameworks for full-arch prostheses under the all-on-four concept remains unclear. The present study applied finite element analysis to examine the behavior of polyetherketoneketone (PEKK) and polyetheretherketone (PEEK) prosthetic frameworks. Materials and Methods A three-dimensional maxillary model received four axially positioned morse-taper implants, over which a polymeric bar was simulated. The full-arch prosthesis was created from a previously reported database model, and the imported geometries were divided into a mesh composed of nodes and tetrahedral elements in the analysis software. The materials were assumed as isotropic, elastic, and homogeneous, and all contacts were considered bonded. A normal load (500 N magnitude) was applied at the occlusal surface of the first left molar after the model was fixed at the base of the cortical bone. The microstrain and von-Mises stress were selected as criteria for analysis. Results Similarities in the mechanical response were observed in both framework for the peri-implant tissue, as well as for stress generated in the implants (263-264 MPa) and abutments (274-273 MPa). The prosthetic screw and prosthetic base concentrated more stress with PEEK (211 and 58 MPa, respectively) than with PEKK (192 and 49 MPa), while the prosthetic framework showed the opposite behavior (59 MPa for PEEK and 67 MPa for PEKK). Conclusion The main differences related to the mechanical behavior of PEKK and PEEK frameworks for full-arch prostheses under the all-on-four concept were reflected in the prosthetic screw and the acrylic base. The superior shock absorbance of PEKK resulted in a lower stress concentration on the prosthetic screw and prosthetic base. This would clinically represent a lower fracture risk on the acrylic base and screw loosening. Conversely, lower stress concentration was observed on PEEK frameworks. Federal University of Espírito Santo Rede Nordeste de Biotecnologia, Espírito Santo Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University, São José dos Campos São Paulo Department of Dentistry University of Taubaté, 09 Operários Street, São Paulo Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University, São José dos Campos São Paulo

Made available in DSpace on 2021-06-25T11:15:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 This study evaluated the stress distribution in five different class II cavities of premolar models restored with conventional or bulk-fill flowable composite by means of finite element analysis (FEA) under shrinkage and occlusal loading. An upper validated premolar model was imported in the software, and five class II cavities with different occlusal extensions and dimensions were prepared: horizontal cavity on the mesial surface (horizontal slot), mesio-occlusal cavity, mesial cavity (vertical slot), tunnel type cavity and direct access cavity. The models were restored with conventional or bulk-fill flowable resin composite. The tested materials were considered as homogeneous, linear, and isotropic. The Maximum Principal Stress criteria was chosen to evaluate the tensile stress results. The lowest shrinkage stress value was observed in the direct access cavity restored with bulk-fill flowable resin composite (36.12 MPa). The same cavity, restored with conventional composite showed a score of 36.14 MPa. The horizontal slot cavity with bulk-fill flowable showed a score of 46.71 MPa. The mesio-occlusal cavity with bulk-fill flowable had a score of 53.10 MPa, while with conventional composite this was 55.35 MPa. Higher shrinkage stress was found in the vertical slot cavity with conventional resin 56.14 MPa, followed by the same cavity with bulk-fill flowable 56.08 MPa. Results indicated that the use of bulk-fill flowable composite resin more significantly decreased the polymerization shrinkage stress magnitude. The larger the cavity and the volume of material necessary to restore the tooth, the greater the residual stress on enamel and dentin tissue. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNESP) Department of Dentistry University of Taubaté (UNITAU) Department of Oral and Maxillo Facial Sciences “Sapienza” University of Rome Department of Neurosciences Reproductive and Odontostomatological Sciences School of Dentistry University of Naples Federico II Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNESP)

Made available in DSpace on 2022-05-01T09:31:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Purpose: Using the microshear bond strength (pSBS) test, this study investigated the bond strength between a hybrid ceramic and the extrinsic characterization layer after different ceramic surface treatments. Materials and Methods: Hybrid ceramic blocks (Vita Enamic, Vita Zahnfabrik) were sectioned and randomly divided into 4 groups (N = 120) according to the surface treatment and aging (n = 15): P: polishing; E: acid etching with HF; A: aluminum oxide blasting; S: self-etching ceramic primer. The specimens were silanized, then cylinders of light-curing characterization material (Vita Enamic Stain, 1.6 mm diameter x 2 mm height) were fabricated, followed by glazing. The specimens were subsequently immersed in distilled water for 24 h and subjected to the pSBS test using a universal testing machine (load cell 0.5 mm/min, 50 kgf) or tested after thermocycling for 10,000 cycles in water (5°C-55°C). After treatment, the specimen surfaces were analyzed using SEM, with failure types defined as adhesive, predominantly adhesive, or cohesive. The data were analyzed by two-way ANOVA followed by Tukey's test (p < 0.05). Results: The most frequent failure type was predominantly adhesive between ceramic and the characterization layer. There were statistically significant differences between the surface treatments (p < 0.05). Thermocycling did not lead to statistically signifcant different results (p > 0.05). For groups P and A, a sharp decrease in SBS was observed. Conclusion: The absence of surface treatment drastically reduced the microshear bond strength between the ceramic and the characterization layer. Conditioning with 5% hydrofluoric acid for 60 s is the most suitable treatment for adhesion of the characterization layer to hybrid ceramic. PhD Student Postgraduate Program in Restorative Dentistry Sao Paulo State University (UNESP) Institute of Science and Technology MSc Student Postgraduate Program in Restorative Dentistry Sao Paulo State University (UNESP) Institute of Science and Technology Professor Postgraduate Program in Restorative Dentistry Sao Paulo State University (UNESP) Institute of Science and Technology Professor Department of Dentistry University of Taubaté (UNITAU) PhD Student Postgraduate Program in Restorative Dentistry Sao Paulo State University (UNESP) Institute of Science and Technology MSc Student Postgraduate Program in Restorative Dentistry Sao Paulo State University (UNESP) Institute of Science and Technology Professor Postgraduate Program in Restorative Dentistry Sao Paulo State University (UNESP) Institute of Science and Technology

Made available in DSpace on 2022-04-28T19:44:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The acquisition and treatment of strain data play an important role in various engineering applications, involving experimental stress analysis on a surface. Its operationalization, however, requires care and equipment that should ensure accuracy in data acquisition and ease of sampling. Deformation data acquisition systems are critical in these cases but still costly when it comes to applications in academic laboratories. This work shows he development of a portable data acquisition system for strain reading using electrical extensometry that can be used to acquire experimental strain data. The developed system has low cost and portability, being suitable to attend data acquisition in remote places. For its development an Arduino UNO R3 board was used in conjunction with an ADS1115 analog-to-digital converter. The converter is required to increase the analog/digital conversion accuracy of the Arduino board and to read electrical voltages in the μV range. In addition, an LCD display was added to the system to show the acquired data instantly. This way, the system does not require the use of a computer to function and can be easily transported. For its measurement, an experiment was carried out with strain gauges glued on the surface of an aluminum beam and connected in a Wheatstone bridge circuit. Also, a numerical model was developed using the LISA finite element program. After testing and comparing experimental, analytical and numerical results, it can be stated that the system was able to acquire data with great precision, reaching its objectives. Departament of Mechanical Engineering University of Taubaté, Rua Daniel Danelli, sn, Jardim Morumbi Department of Mechanical Engineering São Paulo State University (Unesp) Department of Mechanical Engineering São Paulo State University (Unesp)

Made available in DSpace on 2022-04-29T08:36:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background: The objective of this in vitro study was to evaluate the effect of the active application of self-etching ceramic primer (ME&P) on the bond strength of different dental CAD/CAM materials (Lithium Disilicate ceramic (LD), Leucite ceramic (LE), Zirconia reinforced lithium silicate ceramic (ZLS), and Hybrid ceramic (HC)) with thermocycling aging. Material and Methods: The samples were randomly divided into 16 groups (n = 20). Dual resin cement cylinders were made and light cured for 10 s (1.200 mW/cm2) for the shear bond strength test. 3-way ANOVA revealed that the factors were statistically significant (P < 0.05). Results: The aging process had a negative impact on the bond strength for all groups except for Lithium Disilicate, with active application. ZLS and LE showed promising results with high bond strength values for the ME&P active application; however, after aging the bond strength value was significantly reduced. HC showed reduced bond strength values regardless the ME&P application. Conclusions: In order to obtain a durable bond strength, the recommended protocol of 20 s of active application followed by 40 s of sitting time in the self-etching ceramic primer should be followed when using reinforced-glass ceramics as restorative materials. DDS MSc PhD Department of Dentistry University of Taubaté (UNITAU) DDs MSc PhD student Department of Dental Materials and Prosthodontics Sao Paulo State University (Unesp) Institute of Science and Technology, Sao José dos Campos DDS MSc PhD student Department of Dentistry University of Taubaté (UNITAU) DDs MSc PhD Department of Dental Materials and Prosthodontics Sao Paulo State University (Unesp) Institute of Science and Technology, Sao José dos Campos DDs MSc PhD student Department of Dental Materials and Prosthodontics Sao Paulo State University (Unesp) Institute of Science and Technology, Sao José dos Campos DDs MSc PhD Department of Dental Materials and Prosthodontics Sao Paulo State University (Unesp) Institute of Science and Technology, Sao José dos Campos

(1) Background: Trauma is a very common experience in contact sports, however, there is an absence of data regarding the effect of athletes wearing mouthguards (MG) associated with ankylosed maxillary central incisor during a traumatic impact. (2) Methods: To evaluate the stress distribution in the bone and teeth in this situation, models of maxillary central incisor were created containing cortical bone, trabecular bone, soft tissue, root dentin, enamel, periodontal ligament, and antagonist teeth were modeled. One model received a MG with 4-mm thickness. Both models were subdivided into finite elements. The frictionless contacts were used and a nonlinear dynamic impact analysis was performed in which a rigid object hit the model at 1 m&middot, s&minus, 1. For each model, an ankylosed periodontal ligament was simulated totaling 4 different situations. The results were presented in von-Mises stress maps. (3) Results: A higher stress concentration in teeth and bone was observed for the model without a MG and with ankylosed tooth (19.5 and 37.3 MPa, respectively), the most promising mechanical response was calculated for patients with healthy periodontal ligament and MG in position (1.8 and 7.8 MPa, respectively). (4) Conclusions: The MG&rsquo, s use is beneficial for healthy and ankylosed teeth, since it acts by dampening the generated stresses in bone, dentin, enamel and periodontal ligament. However, patients with ankylosed tooth are more prone to root fracture even when the MG is in position compared to a healthy tooth.

Made available in DSpace on 2020-12-12T02:47:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: To evaluate if the bioadhesive polymer (Carbopol 980) could potentiate the protective effect of sodium fluoride with stannous chloride (FS) solution on the control of enamel erosive wear. Methods: Cylindrical bovine enamel specimens were polished and randomly allocated into three groups (n = 60): FS (500 ppm F- +800 ppm Sn2+ - positive control), FS + Carbopol (0.1% Carbopol), and ultrapure water (negative control). A randomized double-blind cross-over in situ model with three phases was used. In each phase, volunteers (n = 15) used a palatal appliance containing 4 specimens: two were submitted to an erosion model (2 h of pellicle formation; immersion in 1% citric acid, pH 2.3, for 5 min, 4x/day; 1 h intervals of saliva exposure; and treatment with the test solutions for 1 min, 2x/day). Besides erosion, the other two specimens were also subjected to abrasion (2x/day, 15 s) with active electric toothbrush, before the treatment with the solutions. After 5 days, enamel surface loss (μm) was evaluated by profilometry. Data were analyzed by two-way RM-ANOVA and Tukey tests (5%). Results: There were significant differences for both challenge and treatment factors. Erosion/abrasion challenge resulted in significantly higher enamel loss than erosion only (p < 0.05). The surface loss values for the erosion/remineralization model were (means ± SL): C = 14.7 ± 5.8b; FS = 9.0 ± 7.5ab; FS + Carbopol = 5.9 ± 3.8a; and for erosion/abrasion: C = 26.6 ± 10.1c; FS = 15.0 ± 8.8b; FS + Carbopol = 12.3 ± 7.9ab. Conclusion: The association of Carbopol to the FS solution significantly protected the enamel against erosive wear, but it was not significantly superior to FS only. Clinical Significance: Under highly erosive and abrasive conditions, rinsing with solutions containing sodium fluoride plus stannous chloride, associated or not with the Carbopol polymer, is an effective approach to control enamel erosive wear. Department of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESP Department of Restorative Dentistry Dental School University of Taubaté Department of Restorative Dentistry São Paulo University-USP Department of Chemical Engineering Polytechnic School São Paulo University-USP Department of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESP

Made available in DSpace on 2021-06-25T10:16:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Mesenchymal stem cells are candidates for therapeutic strategies in periodontal repair due to their osteogenic potential. In this study, we identified epigenetic markers during osteogenic differentiation, taking advantage of the individual pattern of mesenchymal cells of the periodontal ligament with high (h-PDLCs) and low (l-PDLCs) osteogenic capacity. We found that the involvement of non-coding RNAs in the regulation of the RUNX2 gene is strongly associated with high osteogenic potential. Moreover, we evaluated miRs and genes that encode enzymes to process miRs and their biogenesis. Our data show the high expression of the XPO5 gene, and miRs 7 and 22 observed in the l-PDLCs might be involved in acquiring osteogenic potential, suppressing RUNX2 gene expression. Further, an inversely proportional correlation between lncRNAs (HOTAIR and HOTTIP) and RUNX2 gene expression was observed in both l- and h-PDLCs, and it was also related to the distinct osteogenic phenotypes. Thus, our results indicate the low expression of XPO5 in h-PDLC might be the limiting point for blocking the miRs biogenesis, allowing the high gene expression of RUNX2. In accordance, the low expression of miRs, HOTAIR, and HOTTIP could be a prerequisite for increased osteogenic potential in h-PDLCs. These results will help us to better understand the underlying mechanisms of osteogenesis, considering the heterogeneity in the osteogenic potential of PDLCs that might be related to a distinct transcriptional profile of lncRNAs and the biogenesis machinery. Faculty of Dentistry University of Taubaté Program in Environmental and Experimental Pathology Paulista University São Paulo Lab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP – São Paulo State University, São Paulo Department of Pathology Federal University of Minas Gerais Department of Radiology Columbia University Medical Center School of Dentistry Health Science Institute Paulista University Department of Prosthodontics and Periodontics Piracicaba Dental School University of Campinas Lab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP – São Paulo State University, São Paulo CNPq: ODO 154/2019

Objectives The present study examined a technique for reducing dentin permeability through the application of a calcium phosphate (CaP)-based desensitiser with a laser-assisted process and evaluated adhesive-dentin bond strength. Methods: Thirty dentin discs were divided into two groups according to whether the selected desensitiser (TeethMate; Kuraray Noritake) was used prior to dentin bonding. Each group was subdivided into three subgroups (n = 5): A- Adhesive (Single Bond Universal, 3M ESPE), AL- Adhesive + Laser (Nd:YAG 60 mJ) and LAL- Laser + Adhesive + Laser. Dentin permeability values (%) were recorded before and after desensitiser application. Resin composites were placed over the bonded specimens; the latter were aged prior to microtensile bond strength evaluation. Gelatinolytic activity within the hybrid layers was examined with in-situ zymography using confocal laser scanning microscopy. Data were analysed with ANOVA and Tukey test (α = 0.05). Results Significant differences in dentin permeability were identified for all groups (p = 0.00). Both laser treatment (p = 0.182) and desensitiser application (p = 0.687) did not significantly improve dentin bond strength. Ultrastructure of the resin-dentin interface identified presence of calcium phosphate within dentinal tubules. Laser treatment did not affect hybrid layer ultrastructure. Both treatment modalities (intratubular CaP occlusion and laser) had no influence on gelatinolytic activity within hybrid layers. Conclusion Although intratubular CaP occlusion and laser treatment were effective in reducing dentin permeability, they did not affect bond strength, interfacial ultrastructure and gelatinolytic activity within hybrid layers. Clinical relevance Treatment of etched dentin with Nd:YAG Laser at 60 mJ does not adversely affect collagen ultrastructure and gelatinolytic activity within the hybrid layer. The application of a calcium phosphate-based desensitiser to etch dentin does not affect dentin bond strength., Materials science; Biomedical engineering; Bond strength; Calcium phosphate; Dentin permeability; Nd:YAG laser.

Made available in DSpace on 2020-12-12T01:18:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01 Background: Inflammatory/immunological serum markers are useful for the early detection of organ dysfunction, helping the diagnosis of sepsis. Although the detection of blood biomarkers is a standard practice, the use of noninvasive samples (eg saliva) would be beneficial. Aim: To investigate the saliva of hospitalized patients with and without sepsis and identify the levels of inflammatory markers such as C-reactive protein (CRP), procalcitonin (PCT), interleukin 6 (IL-6) and nitric oxide (NO). Methods: Saliva samples were collected from 26 patients in intensive care unit with diagnosis of sepsis and from 26 without sepsis (control). The levels of CRP were determined by using latex agglutination test, whereas those of procalcitonin and IL-6 by ELISA and NO by the Griess reaction. Results: Of 26 patients with sepsis, 14 were males (54%) with a mean age of 63.81 ± 3.48 years. The control group had the same distribution for gender, with mean age 65.04 ± 4.07 years. Sepsis group showed higher salivary concentrations of CRP, PCT, IL-6 and NO, with only levels of IL-6 being statistically different (P =.0001). Conclusions: Patients with sepsis had significantly higher levels of IL-6 in their saliva, suggesting that this biological sample could be useful in the diagnosis of this condition. UNITAU - University of Taubaté UNESP – State of São Paulo University UNISA - University of Santo Amaro HUMANITAS - School of Medical Sciences of São José dos Campos UNESP – State of São Paulo University

Made available in DSpace on 2021-06-25T10:13:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 There is an increased effort on developing novel and active surfaces in order to accelerate their osteointegration, such as nanosized crystalline hydroxyapatite coating (HAnano®). To better understand the biological behavior of osteoblasts grown on HAnano® surface, the set of data was compared with SLActive®, a hydrophilic sandblasted titanium surface. Methodologically, osteoblasts were seeded on both surfaces up to 72 hours, to allow evaluating cell adhesion, viability, and set of genes encoding proteins related with adhesion, proliferation, and differentiation. Our data shows HAnano® displays an interesting substrate to support cell adhesion with typical spread morphologic cells, while SLActive®-adhering cells presented fusiform morphology. Our data shows that the cellular adhesion mechanism was accompanied with upexpression of integrin β1, Fak, and Src, favoring the assembling of focal adhesion platforms and coupling cell cycle progression (upmodulating of Cdk2, Cdk4, and Cdk6 genes) in response to HAnano®. Additionally, both bioactive surfaces promoted osteoblast differentiation stimulus, by activating Runx2, Osterix, and Alp genes. Although both surfaces promoted Rankl gene expression, Opg gene expression was higher in SLActive® and this difference reflected on the Rankl/Opg ratio. Finally, Caspase1 gene was significantly upmodulated in response to HAnano® and it suggests an involvement of the inflammasome complex. Collectively, this study provides enough evidences to support that the nanohydroxyapatite-coated surface provides the necessary microenvironment to drive osteoblast performance on dental implants and these stages of osteogenesis are expected during the early stages of osseointegration. Lab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP-Saõ Paulo State University School of Dentistry University of Taubaté Program in Environmental and Experimental Pathology Paulista University Lab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP-Saõ Paulo State University

Made available in DSpace on 2019-08-28T18:55:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2019 This study evaluated the effect of the CAD/CAM burs experience (newer vs older as consequence of the milling sequence) on fatigue failure load (FFL), number of cycles for failure (CFF), and survival rates of lithium disilicate glass-ceramic simplified restorations adhesively cemented to a dentin analogue substrate. Three sets of CAD/CAM burs were used to mill disc-shaped ceramic specimens (1 bur set - 18 milled discs with 10 mm diameter and 1.5 mm thickness), considering the bur experience as a result of the milling sequence to compose the study groups: G1-6 - discs obtained from the 1st to 6th milling of each bur set; G7-12 - specimens from the 7th to 12th milling; G13-18 - discs from the 13th to 18th. Discs of dentin analogue (G10, 10 mm diameter and 2.0 mm thickness) were made to serve as substrate (base material) and randomly assigned into pairs with the respective ceramic discs. Then, the ceramic discs were adhesively cemented onto the dentin analogue substrate, composing a three-layer specimen that mimics a monolithic restoration of a posterior tooth. Specimens were tested under stepwise fatigue approach: frequency = 20 Hz, 5000 cycles at maximum load of 400 N to accommodate the testing assembly, followed by incremental steps of 200 N with initial load ranging from 10 to 1000 N, to a maximum of 20,000 cycles/each step, until the occurrence of failure (radial crack). FFL and CFF were recorded at the end of the testing and subjected to statistical analysis. Supplementary roughness analysis of the milled surface was performed (n = 18) using a contact profilometer. Residual stress after milling and acid etching were accessed via X-ray Diffractometry analysis. FFL and CFF were not affected by increase on bur experience (no statistical differences among groups), despite that, it affected both Ra and Rz parameters (G1-6 had the smoothest surface). The residual stress concentration was negligible (milling did not induce residual stress concentration). It is concluded that the fatigue behavior of adhesively cemented lithium-disilicate glass-ceramic restorations was not influenced by CAD/CAM bur experience (newer vs older as consequence of the milling sequence), and so the residual stress concentration induced by milling was negligible. [de Andrade, Guilherme Schmitt; Diniz, Vandeberg; Datte, Carlos Eduardo; Bottino, Marco Antonio; de Melo, Renata Marques] Sao Paulo State Univ Unesp, Inst Sci & Technol, Dept Dent Mat & Prosthodont, Sao Jose Dos Campos, Brazil [Venturini, Andressa Bonin; Valandro, Luiz Felipe] Fed Univ Santa Maria UFSM, Fac Odontol, Prosthodont Unit, Oral Sci, Floriano Peixoto 1184, BR-97015372 Santa Maria, RS, Brazil [Rocha Pereira, Gabriel Kalil] Merid Fac IMED, Sch Dent, Passo Fundo, RS, Brazil [Amaral, Marina] Universidade de Taubaté (Unitau) , Dept Dent Prosthet Dent [Bastos Campos, Tiago Moreira] Aeronaut Technol Inst ITA, Dept Phys, Sao Jose Dos Campos, Brazil

Made available in DSpace on 2020-12-12T01:44:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) To better address whether the long noncoding RNAs (lncRNAs) HOTAIR and HOTTIP are mechanosensitive genes, they were investigated in differentially challenged endothelial cells with respect to a circuit of tensional forces, considering the performance of both arterial and venous endothelial cells. We subjected arterial- and venous-obtained endothelial cells to a circuit of tensional forces within a shear stress model in vitro. Real-time quantitative polymerase chain reaction analysis indicated that microRNA (miRNA)-related processing machinery is significantly required in shear stressed arterial endothelial cell metabolism, which orchestrates miRNA (small noncoding RNA) involvement, and their involvement suggests lncRNA involvement. Of lncRNAs HOTAIR and HOTTIP, only HOTAIR was mechanosensitive considering both arterial and venous endothelial cells, presenting a positive correlation between methylation signature and gene expression. Thereafter, using bioinformatics tools, lncRNA HOTAIR was predicted to modulate miRNA185, miRNA-21, and miRNA23b downregulation. We compared the values of gene expression with a Pearson's correlation test, and expected correlations were observed for miRNA185 (r = 0.8664), miRNA-21 (r = 0.8605), and miRNA23b (0.9128). Taken together, these findings clearly show that lncRNA HOTAIR responds to the shear stress and emerges as a novel mechanosensitive gene in endothelial cells. Altogether, this understanding of mechanosensitive transcriptional and posttranscriptional control involving HOTAIR can also lead to new forms of therapeutic intervention for various diseases, as well as new strategies for tissue engineering and regenerative medicine. Laboratory of Bioassays and Cellular Dynamics Department of Chemistry and Biochemistry Institute of Biosciences São Paulo State University - UNESP Division of Dental Biology Department of Dentistry University of Taubaté Laboratory of Bioassays and Cellular Dynamics Department of Chemistry and Biochemistry Institute of Biosciences São Paulo State University - UNESP FAPESP: 2014/22689-3 FAPESP: 2016/01139-0 CNPq: PQ-2

Made available in DSpace on 2020-12-12T01:48:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The understanding of vascular calcification-based mechanism is an urgent pending task in vascular biology and this prompted us to better address this issue by investigating whether DNA methylation mechanism might drive osteogenic marker genes modulation in primary human vascular smooth muscle cells (VSMCs) responding to calcium and phosphate levels overload up to 72 h. Firstly, our data shows this calcifying process recapitulates the molecular repertory of osteogenic biomarkers and specifically requiring RUNX2, Osterix and ALP, BSP genes activations along 72 h in vitro, and this behavior was validated here using other lineages. Conversely, both BMPs 4 and 7 were significantly overexpressed, maybe already as a mechanism in response to RUNX2 and Osterix genes activities identified earlier in response to the calcifying condition, and taken into maintain the calcifying phenotype of VSMCs. Additionally, survival signaling was maintained active and accompanied by a dynamic cytoskeleton rearrangement signaling requiring MAPK and AKT phosphorylations. Moreover, during the contractile-to-calcifying transition phenotype of VSMCs, epigenetic machinery was finely modulated, requiring the translocation of DNMT3B and TET2 into nucleus and this prompted us evaluating whether the profile of osteogenic-related gene promoters’ methylation might contribute with this process. By firstly estimating 5meC/5 hmeC ratio changes, we further specifically show the significance of the epigenetic modulation of Osterix and Bone sialoprotein related gene promoters, presenting a positive correlation between the epigenetic signature of their gene promoters and transcriptional patterns. Altogether, our results show for the first time the importance of epigenetic mechanism on modulating osteogenic gene markers reprogramming during calcifying VSMCs phenotype acquisition, which might drive the genesis of vascular ectopic calcification. Laboratory of Bioassays and Cellular Dynamics of the Department of Chemistry and Biochemistry Institute of Biosciences São Paulo State University - UNESP, Botucatu Department of Biology Dental School University of Taubaté, Taubaté Laboratory of Bioassays and Cellular Dynamics of the Department of Chemistry and Biochemistry Institute of Biosciences São Paulo State University - UNESP, Botucatu

This clinical study was conducted to correlate the levels of endotoxins and culturable bacteria found in primary endodontic infection (PEI) with the volume of root canal determined by using Cone Beam Computed Tomography (CBCT); and to evaluate the bacterial diversity correlating with clinical features. Twenty patients with PEI were selected and clinical features were recorded. The volume (mm3) of root canal was determined by CBCT analysis. Root canal samples were analyzed by using kinetic LAL-assay test to determine the levels of endotoxins and anaerobic technique to determine the bacterial count (CFU/mL). DNA was extracted from all samples to determine bacterial diversity and quantified by using Checkerboard-DNA-DNA- Hybridization. Culturable bacteria and endotoxins were detected in 100% of the root canal samples. Linear regression analysis revealed a correlation between root canal volume and presence of anaerobic bacteria (p

Made available in DSpace on 2022-04-29T08:28:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Aim: In dental implant treatment, there is a demand for mechanically stronger implants. Despite the existence of several studies showing the clinical success of narrow diameter implants, most of them are based on pure titanium (cpTi) alloys. There is a few clinical evidences of the success rate of titanium-zirconium (TiZr) narrow diameter implants. The aim of this study was to evaluate the stress distribution in the peri-implant area of narrow diameter cpTi and TiZr implants under axial and oblique loads. Methods: Photoelastic models were produced using epoxy resin (PL2, Vishay Precision Group) from a master model. The implants (cpTi and TiZr; Straumann AG) had 3.3 mm in diameter and 12 mm in height. Loads of 100 N and 200 N were applied to the abutment at angles of 0° (axial), 10°, 20°, and 30° (oblique). A circular polariscope (Eikonal) was used under dark field white-light configuration. The isochromatic fringes were analyzed in the peri-implant region in 5 areas, using ASTM table with isochromatic fringes; cervical-mesial, cervical-distal, mid-mesial, mid-distal and apical. Results: In general, under axial and oblique loads, the stress in the TiZr implant was lower than in the cpTi implant. The load of 200 N produced the highest stress values in cpTi and TiZr implants. In both implants and loads, the fringes were located more in apical area at all angles evaluated. Conclusion: It can be concluded that for small implants, the load inclination and intensity change the pattern of stress distribution and the cpTi implant exhibited the highest peri-implant stress. Department of Prosthodontics School of Dentistry University of Taubaté Department of Materials and Technology School of Engineering of Guaratinguetá São Paulo State University Department of Materials and Technology School of Engineering of Guaratinguetá São Paulo State University

Made available in DSpace on 2020-12-12T01:20:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Background: This study evaluated the presence of a silica-nylon mesh and two cantilever lengths on the biomechanical behavior of complete-arch implant-supported prostheses. Material and Methods: Twenty-four (24) complete mandibular arch implant-supported prostheses were divided into 4 groups according to the presence of reinforcing mesh (with or without) and the cantilever length (molar-15 mm or premolar-5 mm). The specimens were submitted to strain gauge analysis (30-kgf, 10 s) at different points (molar and premolar). Three-dimensional models were created based on the in vitro specimens, and the results in the bone (microstrain), prostheses (tensile stress), implants and prosthetic screws (von-Mises stress) were evaluated using the finite element method (FEM). All materials were considered homogeneous, isotropic and linear. Strain gauge data were submitted to 3-way analysis of variance and the Tukey test (α=.05). FEM results were qualitatively analyzed using colorimetric graphs. Results: The microstrain magnitude for the prostheses with reinforcement was 519.91±359 and 583.33±661 without reinforcement (p=.001). The microstrain values for loading on the molar was 867.49±784 and on the premolar was 235.75±145. FEM corroborated with the in vitro findings for the bone behavior. The load application in the premolar showed reduced stress concentration, and a significant difference was observed between the presence or absence of the reinforcement for the prostheses. Conclusions: Silica-nylon mesh reduced the peri-implant microstrain and the prosthesis stress regardless of the cantilever extension. For temporary complete-arch implant-supported prostheses, the limitation of the cantilever to the premolar region improves the biomechanical response during load application. Department of Dental Materials and Proshodontics São Paulo State University (Unesp) Institute of Science and Technology Department of Dentistry (Prosthethic Dentistry) University of Taubaté (Unitau) Department of Prosthodontics Brazcubas Education Department of Dental Materials and Proshodontics São Paulo State University (Unesp) Institute of Science and Technology

Made available in DSpace on 2019-09-12T16:53:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2018 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The demand from the automotive industry for lighter and more resistant structures produced at lower costs has shifted the development focus of production processes toward hybrid components. A problem that arises from hybrid components is the necessity to join dissimilar materials, e.g., polymers and metals. A method to achieve this joining involves a process known as heat staking, in which a metal insert is heated and pushed against a thermoplastic surface. At the end of this process, the metal component may not be level with the thermoplastic surface; rather, it may be over flushed, and this discrepancy is known as the Insertion Height. This paper aims to apply the design of experiments and the response surface methodology to develop a model for the Insertion Height, considering the Heating Temperature and the Insertion Time as independent variables. The experiments revealed that the Insertion Height is most affected by the Heating Temperature. There are several combinations of the factors that can keep the Insertion Height within the specifications; therefore, it is possible to increase productivity by decreasing the Insertion Time and to save energy by reducing the Heating Temperature while considering the process constraints and specifications. [Neto, Antonio Faria; Branco Costa, Antonio Fernando] Sao Paulo State Univ UNESP, Sch Engn, Av Dr Ariberto Pereira Cunha 333, BR-12516410 Guaratingueta, SP, Brazil [Neto, Antonio Faria; de Lima, Michel Floriano] Universidade de Taubaté (Unitau) Unitau, Profess Masters Degree Program, R Daniel Danelli S-N, BR-12060440 Taubate, SP, Brazil

Made available in DSpace on 2018-12-11T17:13:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Statement of problem: Complete-arch implant-supported prostheses without a framework have a high risk of failure: a straightforward and inexpensive reinforcement material, such as nylon mesh, could improve their longevity. Purpose: The purpose of this in vitro study was to evaluate a nylon-silica mesh compound on the fracture strength of acrylic resin and the fracture load of complete-arch implant-supported prostheses. Material and methods: Twenty-four complete mandibular arch implant-supported prostheses were divided into 2 groups according to cantilever length (molar and premolar) and subdivided into another 2 subgroups according to the presence or absence of reinforcing mesh. The specimens were submitted to a maximum load-to-fracture test in a universal testing machine, with a 100-N load cell, a 2 mm/min crosshead speed, and a spherical metal tip diameter of 4 mm at different points (molar and premolar). These were submitted to 1-way analysis of variance for repeated measurement and the post hoc Tukey multiple comparison test (α=.05). Results: The mean maximum load ±standard deviation for the molar group was 393.4 ±95.0 N with reinforcement and 305.4 ±76.3 N without reinforcement (P=.02); and for the premolar group was 1083.3 ±283.7 N with reinforcement and 605.3 ±90.5 N without reinforcement (P=.001). Conclusions: Reinforcement with nylon mesh increased the mean maximum load of implant-supported complete-arch prostheses at both cantilever lengths. The cantilever to the premolar (5 mm) presented the highest maximum load values to fracture. Postdoctoral Program Institute of Aeronautics Technology (ITA) São José dos Campos Dental Prostheses Department of Dentistry University of Taubaté (UNITAU) Associate Professor Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) Institute of Science and Technology São José dos Campos Researcher Engineering Applications Laboratory Institute of Aeronautics Technology (ITA) São José dos Campos Associate Professor Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) Institute of Science and Technology São José dos Campos FAPESP: 2011/07334-6

Made available in DSpace on 2018-12-11T17:16:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 This study evaluated the prophylactic effects of the live or heat-killed probiotic strain Lactobacillus rhamnosus ATCC 7469 in Galleria mellonella, inoculated with Staphylococcusaureus or Escherichia coli. L. rhamnosus suspension was prepared and a part of it was autoclaved to obtain heat-killed lactobacilli. The larvae were inoculated of these suspensions and pathogenic. The survival of the larvae was observed during 7 days and after 24 h of inoculation haemocytes counted, melanization and nitric oxide production were analyzed. Larvae survival rate increased in the group inoculated with heat-killed L. rhamnosus, however, with no statistical difference. There was a significant increase in total haemocyte counts in all test groups. Haemolymph melanization and nitric oxide production were higher in the group inoculated with L. rhamnosus and infected with S. aureus. It was concluded that, in this model of infection, heat-killed L. rhamnosus ATCC 7469 promoted greater protection in Galleria mellonella infected with S. aureus or E. coli. Department of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Paulista State University/UNESP, Av, Engenheiro Francisco José Longo, 777-Jardim São Dimas Bioscience Basic Institute University of Taubaté, Rua Expedicionário Ernesto Pereira, 3 Department of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Paulista State University/UNESP, Av, Engenheiro Francisco José Longo, 777-Jardim São Dimas

716749 Made available in DSpace on 2019-09-11T20:51:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2015 This study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0. 05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0. 05) but not IL-6 (P > 0. 05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0. 05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses. © 2015 Adeline Lacerda Jorjão et al. Jorjão, A.L., Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, Brazil De Oliveira, F.E., Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, Brazil Leão, M.V.P., Bioscience Basic Institute, University of Taubaté, Street Expedicionário Ernesto Pereira 3, Taubaté, SP, 12020-130, Brazil Carvalho, C.A.T., Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, Brazil Jorge, A.O.C., Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, Brazil De Oliveira, L.D., Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, São José dos Campos, SP, 12245-000, Brazil

Made available in DSpace on 2019-09-12T16:53:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2017 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Due to the growing number of multi-resistant Candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. This study evaluated the immunomodulation and anti-Candida activity of Lactobacillus rhamnosus (LR), Lactobacillus acidophilus and Lactobacillus paracasei suspensions, either single- or multiple-strain, in mouse macrophages (RAW 264.7) and Galleria mellonella (GM). Mouse macrophages were activated by different lactobacilli suspensions and challenged with C albicans (CA). Tumor necrosis factor (TNF)-alpha, interleukin IL-beta, IL-6 and IL-17 production and cell viability were investigated. LR was the best suspension for stimulating all evaluated cytokines and thus was used in subsequent in vivo assays. Two C albicans clinical strains, CA21 and CA60, were then added to the GM assays to further confirm the results. LR suspension was injected into the larvae 24 h before challenging with CA. Survival curve, CFU per larva and hemocytes were counted. In the GM, the LR suspension increased the survival rate and hemocyte counts and decreased the CFU per larva counts for all groups. Lactobacilli suspensions presented strain-dependent immunomodulation; however, single suspensions showed better results. Anti-Candida activity was demonstrated by decreased Candida counts in the GM with the use of LR. (C) 2017 Elsevier Ltd. All rights reserved. [de Oliveira, Felipe Eduardo; Rossoni, Rodnei Dennis; de Barros, Patricia Pimentel; Begnini, Barbara Evelyn; Junqueira, Juliana Campos; Cardoso Jorge, Antonio Olavo; de Oliveira, Luciane Dias] Sao Paulo State Univ Unesp, Dept Biosci & Oral Diag, Inst Sci & Technol, Ave Engn Francisco Jose Longo 777, BR-12245000 Sao Paulo, Brazil [Pereira Leao, Mariella Vieira] Universidade de Taubaté (Unitau), Sch Med, Inst Basic Biosci, Av Tiradentes 500, BR-12030180 Sao Paulo, Brazil

Made available in DSpace on 2015-03-19T16:20:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-12-01Bitstream added on 2015-03-19T16:29:20Z : No. of bitstreams: 1 S1678-77572014000600516.pdf: 2130663 bytes, checksum: e6e83a32b5a40756bac3062d22f899b9 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) ObjectiveEvaluate the thickness and the marking quality of different occlusal contact registration strips (OCRS) and a possible correlation between them. Material and MethodThe following OCRS were selected: Accufilm II, BK20, BK21, BK22, BK23, BK28, and BK31. The thickness was measured in three points of the OCRS with an electronic measuring device (TESA), and the mean was calculated. To produce the marks on the strips, composite resin specimens were adapted to a universal testing machine (Versat 2000) with 40 kgf load cell at a speed of 1.0 mm/min. The mark images were photographed with a stereoscopic microscope (Stemi SV11) and processed and analyzed by the 550-Leica Qwin® analyzer. ResultValues (μm) found in the 1st and 2nd thickness measurements were: Accufilm II - 16.4 and 14.2; BK20 - 10.0 and 8.1; BK21 - 9.5 and 8.0; BK22 - 9.7 and 8.7; BK23 - 9.8 and 7.9; BK28 - 12.8 and 10.0; and BK31 - 8.4 and 8.0, respectively. The mean (mm2) values found in the mark areas were: Accufilm II - 0.078; BK20 - 0.035; BK21 - 0.045; BK22 - 0.012; BK23 - 0.022; BK28 - 0.024; and BK31 - 0.024. The results were submitted to the Kruskal-Wallis (p

Made available in DSpace on 2019-09-12T16:53:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2018 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study aimed to investigate the influence of Lactobacillus rhamnosus intake on the development of candidiasis and cytokines release. Candida suspensions were inoculated into the oral cavity of experimentally immunosuppressed mice for candidiasis induction. The animals were divided into experimental groups: candidiasis with no probiotic intake (F), candidiasis with probiotic intake during Candida inoculation (FP), and candidiasis with probiotic intake 14 days before inoculation with Candida (FPP); and control groups: (C), (CP), and (CPP) without inducing candidiasis with probiotic intake in the same manner as groups F, FP, and FPP, respectively. After these periods, samples were collected from the oral cavity for yeast counts and, after euthanasia, the tongues of the animals were removed for histological analysis. Sera samples were also collected for analysis of IL-1 beta, TNF-alpha, INF-gamma, IL-12, IL-4, and IL-10. FP group showed lower Candida counts in the oral cavity, and the presence of Candida was almost not detected in FPP group. In tissues, the counts of fungi were significantly lower in FPP group, followed by FP. Groups that consumed probiotics also had lower histological and inflammatory infiltrates compared to F. Cytokines analysis demonstrated low concentrations of TNF-alpha, IL-12, IL-4, and IL-10 in all the groups, and no statistical difference between them. The production of IL-6 could be better detected, and the experimental groups that consumed the probiotic showed significant lower levels of this cytokine. The results suggest that L. rhamnosus intake, especially preventively, may avoid or decrease the development of candidiasis in immunosuppressed mice. This work adds scientific evidences that probiotics intake can avoid the development of candidiasis. [Pereira Leao, Mariella Vieira; Goncalves e Silva, Celia Regina; Ferreira dos Santos, Silvana Soleo] Universidade de Taubaté (Unitau), Biosci Basic Inst, Av Tiradentes,500 Bom Conselho, BR-12030180 Taubate, SP, Brazil [Aureliano Tavares, Talita Angelina; Junqueira, Juliana Campos; de Oliveira, Luciane Dias; Cardoso Jorge, Antonio Olavo] Univ Estadual Paulista, Inst Sci & Technol, Dept Biosci & Oral Diag, UNESP, Av Engn Francisco Jose Longo,777 Jardim Sao Dimas, BR-12245000 Sao Jose Dos Campos, SP, Brazil

Made available in DSpace on 2019-09-12T16:25:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Desequilíbrios na microbiota residente podem promover o crescimento de microrganismos oportunistas, como as leveduras do gênero Candida, e o desenvolvimento de doenças, especialmente na população idosa. Este estudo investigou se o consumo do probiótico Yakult LB® (Lactobacillus casei e Bifidobacterium breve) era capaz de influenciar na resposta imune secretória anti-Candida e na presença destes microrganismos na cavidade bucal de 42 idosas saudáveis. Amostras de saliva foram coletadas antes e depois do consumo do probiótico por 30 dias, 3 vezes por semana. As amostras foram semeadas em Agar Saboraud Dextrose com cloranfenicol, as unidades formadoras de colônias (UFC/mL) foram contadas e as espécies de Candida foram identificadas. A análise de IgA anti-Candida foi realizada pela técnica ELISA. Os resultados demonstraram redução na prevalência de Candida (de 92,9% para 85,7%), na contagem de UFC/mL (p≤0,05) e no número de espécies não-albicans, depois do consumo do probiótico. As análises imunológicas mostraram um aumento significativo dos níveis de IgA anti-Candida (p≤0,05). Concluindo, as bactérias probióticas reduziram significantemente a quantidade de Candida na cavidade bucal dos idosos e aumentaram a resposta imune secretória específica para esta levedura, sugerindo a possibilidade de sua utilização no controle da candidose bucal. Imbalance in the resident microbiota may promote the growth of opportunistic microorganisms, such as yeasts of Candida genus and the development of diseases, especially in aged people. This study evaluated whether the consumption of the probiotic Yakult LB® (Lactobacillus casei and Bifidobacterium breve) was able to influence on the specific immunological response against Candida and on the presence of these yeasts in the oral cavity of 42 healthy aged individuals. Saliva samples were collected before and after the probiotic use for 30 days, 3 times a week. The samples were plated in Dextrose Saboraud Agar with chloramphenicol, the colony-forming units (CFU/mL) were counted and the Candida species were identified. Anti-Candida IgA analysis was conducted using the ELISA technique. ANOVA and Student's t-test were used for normally distributed data and the Wilcoxon test was used for data with non-normal distribution (α=0.05). The results showed a statistically significant reduction (p

Made available in DSpace on 2013-08-12T18:13:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-09-01 Made available in DSpace on 2013-09-30T18:34:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:31Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T14:04:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-09-01 Clinical benefits of probiotics have been clearly reported in different gastrointestinal disorders, many of them caused by enterobacteria. The oral cavity is a port of entry and can be an important reservoir of these microorganisms. This work evaluated whether consumption of probiotics was able to influence the presence of enterobacteria in the oral cavity and the specific secretory response against these microorganisms. Saliva samples of healthy individuals were collected and plated in Mac-Conkey agar. Carriers of Gram-negative, rod-shaped microorganisms in the oral cavity were selected and instructed to use the probiotic Yakult LB for 20 days. Saliva was then collected and enterobacteria species were identified using the API 20 E system and by ELISA using anti-enterobacteria IgA. The results showed reduction in the prevalence of enterobacteria, but no significant changes in enterobacterial counts (log CFU/mL; p = 0.3457). The species most frequently isolated were Enterobacter cloacae and Klebsiella oxytoca, both before and after probiotic consumption. No significant changes were observed in anti-enterobacteria IgA levels. In conclusion, probiotic consumption had some influence on enterobacterial presence in the oral cavity, but did not affect enterobacterial counts or the specific immune secretory response against them. Univ Taubate, Biosci Basic Inst, Taubate, SP, Brazil Unesp São Paulo State Univ, Dept Biosci & Oral Diag, Sch Dent Sao Jose Dos Campos, Sao Jose Dos Campos, SP, Brazil Unesp São Paulo State Univ, Dept Biosci & Oral Diag, Sch Dent Sao Jose Dos Campos, Sao Jose Dos Campos, SP, Brazil

Made available in DSpace on 2013-08-12T18:46:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Made available in DSpace on 2013-08-12T18:46:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T18:34:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T18:34:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:30Z No. of bitstreams: 0 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:30Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T14:04:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Made available in DSpace on 2014-05-20T14:04:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Simvastatin, a drug used to lower blood cholesterol, has been reported to have an anabolic effect on bone. The purpose of this study was to evaluate the influence of simvastatin and demineralized bovine bone matrix (DBBM) on the repair of rat calvarial defects. Defects of 5 mm were created in 64 rats, divided into four groups: no local treatment (control); treatment with DBBM (DBBM); treatment with a combination of simvastatin solution (2.2 mg/50 mu l) and DBBM (DBBMSIM-1); and treatment with simvastatin solution (0.5 mg/50 mu l) and DBBM (DBBMSIM-2). Animals were sacrificed on postoperative day 30 or 60, after which the calvariae were X-rayed and prepared for histomorphometric evaluation. The data were submitted to statistical analysis (p < 0.05). Xrays revealed that, on postoperative day 30, animals treated with a lower dose of simvastatin presented the lowest bone density, whereas on postoperative day 60 the use of simvastatin, regardless of the dose, resulted in lower density than that observed in control and DBBM group samples. Histomorphometric analysis revealed that, on postoperative day 30, both DBBM and DBBMSIM-1 had a negative impact on bone formation. on postoperative day 60, none of the combinations tested impaired bone repair. These results showed that the association between DBBM and simvastatin had a negative impact on bone repair. UNITAU Univ Taubate, Dept Dent, Taubate, SP, Brazil UNESP Univ Estadual Paulista, Sao Jose dos Campos Sch Dent, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, Brazil UNESP Univ Estadual Paulista, Sao Jose dos Campos Sch Dent, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, Brazil FAPESP: 07/55508-8