1. Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts
- Author
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Félix Gremonprez, Olivier De Wever, Glenn Wagemans, Laurine Verset, Marc Bracke, Wim Ceelen, Tom Boterberg, Elly De Vlieghere, Pieter Demetter, Christian Gespach, and Joke Tommelein
- Subjects
Oncology ,Colorectal cancer ,THERAPY ,Gastroenterology ,Metastasis ,Orthotopic ,Mice ,0302 clinical medicine ,Intussusception (medical disorder) ,Medicine and Health Sciences ,Medicine ,IN-VIVO ,Gastrointestinal tract ,Age Factors ,Middle Aged ,NUDE-MICE ,3. Good health ,Tumor Burden ,030220 oncology & carcinogenesis ,Heterografts ,030211 gastroenterology & hepatology ,Female ,Colorectal Neoplasms ,Research Paper ,medicine.medical_specialty ,mouse model ,INHIBITION ,Context (language use) ,colorectal cancer ,Mouse model ,03 medical and health sciences ,In vivo ,Internal medicine ,Cell Line, Tumor ,Animals ,Humans ,In patient ,ULCER SYNDROME ,orthotopic ,Rectal prolapse ,business.industry ,COLO320DM ,Rectal Prolapse ,medicine.disease ,ENDOTHELIAL-CELLS ,HUMAN-COLON-CARCINOMA ,Disease Models, Animal ,METASTASIS ,INTUSSUSCEPTION ,GROWTH-FACTOR RECEPTOR ,business ,Psychiatrie - Abstract
In patients with rectal prolapse is the prevalence of colorectal cancer increased, suggesting that a colorectal tumor may induce rectal prolapse. Establishment of tumor xenografts in immunodeficient mice after orthotopic inoculations of human colorectal cancer cells into the caecal wall is a widely used approach for the study of human colorectal cancer progression and preclinical evaluation of therapeutics. Remarkably, 70% of young mice carrying a COLO320DM caecal tumor showed symptoms of intussusception of the large bowel associated with intestinal lumen obstruction and rectal prolapse. The quantity of the COLO320DM bioluminescent signal of the first three weeks post-inoculation predicts prolapse in young mice. Rectal prolapse was not observed in adult mice carrying a COLO320DM caecal tumor or young mice carrying a HT29 caecal tumor. In contrast to HT29 tumors, which showed local invasion and metastasis, COLO320DM tumors demonstrated a non-invasive tumor with pushing borders without presence of metastasis. In conclusion, rectal prolapse can be linked to a non-invasive, space-occupying COLO320DM tumor in the gastrointestinal tract of young immunodeficient mice. These data reveal a model that can clarify the association of patients showing rectal prolapse with colorectal cancer., SCOPUS: ar.j, info:eu-repo/semantics/published
- Published
- 2016