65 results on '"Tuchiya K"'
Search Results
2. The effect of eicosapentaenoic acid on renal function and volume in patients with ADPKD
- Author
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Higashihara, E., primary, Nutahara, K., additional, Horie, S., additional, Muto, S., additional, Hosoya, T., additional, Hanaoka, K., additional, Tuchiya, K., additional, Kamura, K., additional, Takaichi, K., additional, Ubara, Y., additional, Itomura, M., additional, and Hamazaki, T., additional
- Published
- 2008
- Full Text
- View/download PDF
3. Detection of antibodies to equine arteritis virus in horse sera using recombinant chimaeric N/GL protein
- Author
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Turan, N., primary, Ekici, H., additional, Yilmaz, H., additional, Kondo, T., additional, Hasoksuz, M., additional, Sato, I., additional, Tuchiya, K., additional, and Fukunaga, Y., additional
- Published
- 2007
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4. Th1/Th2 balance in childhood idiopathic nephrotic syndrome
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Kaneko, K., primary, Tuchiya, K., additional, Fujinaga, S., additional, Kawamura, R., additional, Ohtomo, Y., additional, Shimizu, T., additional, and Yamashiro, Y., additional
- Published
- 2002
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5. Effect of loading Mode on High Temperature Fatigue Crack propagation along Superalloy/Coating Interface
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Okazaki, M., primary, Tuchiya, K., additional, and Harada, Y., additional
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- 2000
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6. Design and Testing of a Prototype De-NOxSystem for 100 kVA Diesel Engine Generator using a Silent Discharge Reactor
- Author
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YOSHIOKA, Y., primary, YUKITAKE, T., additional, NAKAI, M., additional, SOUMA, K., additional, TUCHIYA, K., additional, ANNOU, K., additional, and HORI, Y., additional
- Published
- 1998
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7. 3 Dimensional imaging of the labyrinth using 3 DFSE and a pair of surface coil. : Evaluation of dual phased array coil. (1st report)
- Author
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Amano, T., primary, Tuchiya, K., additional, Ito, K., additional, kurita, M., additional, kamiya, M., additional, Kusakabe, Y., additional, Takehara, Y., additional, and Nozaki, A., additional
- Published
- 1996
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8. MR Cholangio-Pancreatography using a pair of surface coil : Evaluation of dual phased array coil. (2nd report)
- Author
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Ito, K., primary, Tuchiya, K., additional, Amano, T., additional, kurita, M., additional, kamiya, M., additional, Kusakabe, Y., additional, Takehara, Y., additional, and Nozaki, A., additional
- Published
- 1996
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9. Wire-directed detachable balloon. Work in progress.
- Author
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Makita, K, primary, Furui, S, additional, Machida, T, additional, Yamauchi, T, additional, Tuchiya, K, additional, Irie, T, additional, and Takenaka, E, additional
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- 1991
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10. Molecular cloning and expression of the cDNA encoding feline granulocyte colony-stimulating factor
- Author
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Yamamoto, A., Iwata, A., Tuchiya, K., Katsumata, A., Oishi, K., Saito, T., Tsujimoto, H., Hasegawa, A., and Ueda, S.
- Published
- 2001
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11. Detection of antibodies to equine arteritis virus in horse sera using recombinant chimaeric N/GL protein.
- Author
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Turan, N., Ekici, H., Yilmaz, H., Kondo, T., Hasoksuz, M., Sato, I., Tuchiya, K., and Fukunaga, Y.
- Subjects
HORSE diseases ,IMMUNOGLOBULINS ,ARTERITIS ,VIRUSES ,DETECTORS ,VETERINARY medicine ,RESEARCH ,PROTEINS - Abstract
The article discusses a study detecting the antibodies in equine arteritis virus (EAV) in horse sera through recombinant chimaeric N/G
L protein. It discusses the case of a Turkish horse sera analyzed for the presence of antibodies to EAV using the protein. The study involved 63 horses, which were clinically examined, with the recombinant chimaeric protein employed as an antigen. Discussions on several serological tests such as virus neutralization used to detect EAV are provided.- Published
- 2007
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12. Development of an electroplated polishing tape applying electrodeposited nickel foil method
- Author
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Kentaro Miyazaki, Tani, Y., Kamimura, Y., and Tuchiya, K.
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Mechanics of Materials ,Mechanical Engineering ,Industrial and Manufacturing Engineering
13. Deformation method for surgery simulation using voxel space automata.
- Author
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Sakamoto, Y., Tuchiya, K., and Kato, M.
- Published
- 1999
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14. Long-term follow-up characteristics of patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) receiving chronic hemodialysis at a single center.
- Author
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Miyabe Y, Karasawa K, Takabe T, Ogura S, Sugiura N, Kyoda M, Ono W, Akiyama K, Tanaka N, Moriyama T, Hanafusa N, Uchida K, Tuchiya K, and Nitta K
- Subjects
- Adult, Aged, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis diagnosis, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis immunology, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis mortality, Cause of Death, Disease Progression, Female, Follow-Up Studies, Humans, Kidney Diseases diagnosis, Kidney Diseases immunology, Kidney Diseases mortality, Male, Middle Aged, Recurrence, Retrospective Studies, Risk Factors, Steroids adverse effects, Time Factors, Treatment Outcome, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis therapy, Kidney Diseases therapy, Renal Dialysis adverse effects, Renal Dialysis mortality, Steroids administration & dosage
- Abstract
Background: The clinical characteristics and treatment of patients with antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) after initiating chronic hemodialysis remain unknown., Methods: We retrospectively enrolled 11 adult patients with AAV receiving chronic hemodialysis in our hospital from 2000-2016. We collected data describing each patient's clinical findings and treatment before and after initiating hemodialysis. Patients with AAV with and without post-hemodialysis AAV relapse were compared statistically., Results: The average observation period was 6.8 ± 4.1 years, and the interval between diagnosis and initiating chronic hemodialysis was 1.9 ± 2.6 years. Before initiating chronic hemodialysis, five patients (45%) experienced 12 AAV relapses, with diagnoses made serologically or symptomatically. After initiating chronic hemodialysis, four patients experienced nine relapses, with no significant difference between the number of relapses and the number of patients experiencing relapse (p = 0.067 and 0.083, respectively). For patients' entire clinical courses before initiating chronic hemodialysis, the average steroid dose was 11.6 ± 6.9 g/y. Comparing before and after initiating chronic hemodialysis, the steroid dose decreased significantly to 3.3 ± 1.4 g/y after initiating chronic hemodialysis (p = 0.0012). Two of 11 patients died of serious infections after initiating chronic hemodialysis., Conclusions: Our results showed that the number of relapses tended to be lower despite a significantly different lower amount of steroid after initiating hemodialysis compared with before initiating hemodialysis, and the burn-out phenomenon specific to uremic patients was inferred. We believe that early tapering of steroids should be considered to avoid death rather than focusing only on relapse.
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- 2020
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15. The reoxygenation of hypoxia and the reduction of glucose metabolism in head and neck cancer by fractionated radiotherapy with intensity-modulated radiation therapy.
- Author
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Okamoto S, Shiga T, Yasuda K, Watanabe S, Hirata K, Nishijima KI, Magota K, Kasai K, Onimaru R, Tuchiya K, Kuge Y, Shirato H, and Tamaki N
- Subjects
- Adult, Aged, Dose Fractionation, Radiation, Down-Regulation radiation effects, Female, Head and Neck Neoplasms diagnostic imaging, Humans, Male, Middle Aged, Misonidazole analogs & derivatives, Misonidazole pharmacokinetics, Positron-Emission Tomography methods, Radiopharmaceuticals pharmacokinetics, Reproducibility of Results, Sensitivity and Specificity, Treatment Outcome, Fluorodeoxyglucose F18 pharmacokinetics, Head and Neck Neoplasms metabolism, Head and Neck Neoplasms radiotherapy, Oxygen metabolism, Radiotherapy, Conformal methods, Tumor Hypoxia radiation effects
- Abstract
Purpose: The purpose of this study was to prospectively investigate reoxygenation in the early phase of fractionated radiotherapy and serial changes of tumoricidal effects associated with intensity-modulated radiation therapy (IMRT) in patients with head and neck cancer (HNC) using F-18 fluoromisonidazole (FMISO) PET and F-18 fluorodeoxyglucose (FDG) PET., Methods: Patients with untreated HNC underwent FMISO-PET and FDG-PET studies prospectively. A PET evaluation was conducted before each IMRT (Pre-IMRT), during IMRT (at 30 Gy/15 fr) (Inter-IMRT), and after completion of IMRT (70 Gy/35 fr) (Post-IMRT). FMISO-PET images were scanned by a PET/CT scanner at 4 h after the FMISO injection. We quantitatively analyzed the FMISO-PET images of the primary lesion using the maximum standardized uptake (SUVmax) and tumor-to-muscle ratio (TMR). The hypoxic volume (HV) was calculated as an index of tumor hypoxia, and was defined as the volume when the TMR was ≥ 1.25. Each FDG-PET scan was started 1 h after injection. The SUVmax and metabolic tumor volume (MTV) values obtained by FDG-PET were analyzed., Results: Twenty patients finished the complete PET study protocol. At Pre-IMRT, 19 patients had tumor hypoxia in the primary tumor. In ten patients, the tumor hypoxia disappeared at Inter-IMRT. Another seven patients showed the disappearance of tumor hypoxia at Post-IMRT. Two patients showed tumor hypoxia at Post-IMRT. The FMISO-PET results showed that the reduction rates of both SUVmax and TMR from Pre-IMRT to Inter-IMRT were significantly higher than the corresponding reductions from Inter-IMRT to Post-IMRT (SUVmax: 27 % vs. 10 %, p = 0.025; TMR: 26 % vs. 12 %, p = 0.048). The reduction rate of SUVmax in FDG-PET from Pre-IMRT to Inter-IMRT was similar to that from Inter-IMRT to Post-IMRT (47 % vs. 48 %, p = 0.778). The reduction rate of the HV in FMISO-PET from Pre-IMRT to Inter-IMRT tended to be larger than that from Inter-IMRT to Post-IMRT (63 % vs. 40 %, p = 0.490). Conversely, the reduction rate of the MTV in FDG-PET from Pre-IMRT to Inter-IMRT was lower than that from Inter-IMRT to Post-IMRT (47 % vs. 74 %, p = 0.003)., Conclusions: Both the intensity and the volume of tumor hypoxia rapidly decreased in the early phase of radiotherapy, indicating reoxygenation of the tumor hypoxia. In contrast, the FDG uptake declined gradually with the course of radiotherapy, indicating that the tumoricidal effect continues over the entire course of radiation treatment.
- Published
- 2016
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16. Preferable existence of polyunsaturated lysophosphatidic acids in human follicular fluid from patients programmed with in vitro fertilization.
- Author
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Yamamoto J, Omura M, Tuchiya K, Hidaka M, Kuwahara A, Irahara M, Tanaka T, and Tokumura A
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- Female, Humans, Phosphoric Diester Hydrolases metabolism, Fatty Acids, Unsaturated chemistry, Fertilization in Vitro, Follicular Fluid metabolism, Lysophospholipids chemistry, Lysophospholipids metabolism
- Abstract
Lysophosphatidic acid (LPA) exerts diverse physiological effects on various types of animal cells, including reproductive cells, through its binding to six LPA receptors. We previously found that LPA promoted maturation of the nucleus and cytoplasm of mouse and hamster oocytes surrounded by cumulus cells in vitro. Using gas-liquid chromatography, we previously reported detection of several species of LPA by analyzing the fatty acid methyl esters derived from thin layer chromatography-purified LPA in lipid extract from incubated follicular fluids programmed with in vitro fertilization. In this study using liquid chromatography- tandem mass spectrometry, we directly detected high levels of linoleoyl, arachidonoyl, and docosahexaenoyl LPAs in human follicular fluid. This unique molecular species composition of LPA was suggested to be due to a balance between the low LPA-degrading activity and high LPA-producing activity of autotaxin in human follicular fluid. Our results suggest that polyunsaturated LPAs produced by autotaxin in human follicular fluid exert unknown physiological effects on cumulus cells., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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17. Complete Genome Sequence of the Avian Paramyxovirus Serotype 5 Strain APMV-5/budgerigar/Japan/TI/75.
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Hiono T, Matsuno K, Tuchiya K, Lin Z, Okamatsu M, and Sakoda Y
- Abstract
Here, we report the complete genome sequence of the avian paramyxovirus serotype 5 strain APMV-5/budgerigar/Japan/TI/75, which was determined using the Illumina MiSeq platform. The determined sequence shares 97% homology and similar genetic features with the previously known genome sequence of avian paramyxovirus serotype 5 strain APMV-5/budgerigar/Japan/Kunitachi/74., (Copyright © 2016 Hiono et al.)
- Published
- 2016
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18. Mutations in the spike gene of porcine epidemic diarrhea virus associated with growth adaptation in vitro and attenuation of virulence in vivo.
- Author
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Sato T, Takeyama N, Katsumata A, Tuchiya K, Kodama T, and Kusanagi K
- Subjects
- Animals, Chlorocebus aethiops, Coronavirus M Proteins, Coronavirus Nucleocapsid Proteins, DNA Mutational Analysis, Molecular Sequence Data, Nucleocapsid Proteins, Porcine epidemic diarrhea virus pathogenicity, RNA, Viral genetics, Sequence Analysis, DNA, Serial Passage, Spike Glycoprotein, Coronavirus, Vero Cells, Viral Matrix Proteins genetics, Virulence, Adaptation, Biological, Membrane Glycoproteins genetics, Mutation, Missense, Porcine epidemic diarrhea virus genetics, Porcine epidemic diarrhea virus growth & development, Viral Envelope Proteins genetics
- Abstract
Previously, we have reported that a serial passage of 83P-5 strain of porcine epidemic diarrhea virus (PEDV) in Vero cells resulted in a growth adaptation of the virus in cultured cells at the 22nd passage. In this study, we further maintained the 83P-5 in Vero cells up to the 100th passage and analyzed changes in the spike (S), membrane (M), and nucleocapsid (N) gene sequences and pathogenicity of the virus at the 34th, 61st, and 100th passage levels. Sequence analyses revealed a strong selection for the S gene of 83P-5 in Vero cells, and virtually all mutations occurring at the 34th and 61st passages had been carried over to the 100th-passaged virus. In contrast, the viral M and N genes showed a strong conservation during the serial passage. Pigs experimentally infected with the 34th- or 61st-passaged virus, but not the 100th-passaged virus, exhibited diarrhea, indicating an attenuation of the 83P-5 at the 100th passage. Interestingly, S protein of the attenuated 100th-passaged 83P-5 showed a remarkable sequence similarity to that of previously reported DR-13 strain of attenuated PEDV that also had been established by serial passage in Vero cells. Further studies will be required to define whether the mutations in the S gene of 83P-5 that had been selected and accumulated during the serial passages are indeed the causalities of the growth adaptation in vitro and the attenuation of virulence in vivo.
- Published
- 2011
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19. Proventricular dilatation disease associated with avian bornavirus infection in a Citron-crested Cockatoo that was born and hand-reared in Japan.
- Author
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Ogawa H, Sanada Y, Sanada N, Kudo M, Tuchiya K, Kodama T, and Uetsuka K
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- Amino Acid Sequence, Animals, Base Sequence, Bornaviridae genetics, Dilatation, Pathologic pathology, Dilatation, Pathologic virology, Fatal Outcome, Female, Histocytochemistry veterinary, Japan, Molecular Sequence Data, Mononegavirales Infections pathology, Mononegavirales Infections virology, Phylogeny, Proventriculus virology, RNA, Bacterial chemistry, RNA, Bacterial genetics, Reverse Transcriptase Polymerase Chain Reaction veterinary, Sequence Alignment, Bird Diseases pathology, Bird Diseases virology, Bornaviridae isolation & purification, Cockatoos, Dilatation, Pathologic veterinary, Mononegavirales Infections veterinary, Proventriculus pathology
- Abstract
A 5-month-old female Citron-crested Cockatoo (Cacatua sulphurea citrinocristata) that was born and hand-reared in Japan died with suspected proventricular dilatation disease (PDD). Macroscopic and microscopic examinations of the bird revealed characteristic features of PDD, i.e., distention of the proventriculus and infiltration of lymphocytes and plasma cells in ganglia of various organs and in central and peripheral nerves. A linkage of this PDD case to infection with avian bornavirus (ABV) was documented by RT-PCR amplification of the virus genomes from the affected bird. Phylogenetic analysis revealed that the ABV identified in this study clustered into the genotype 2, which is one of the dominant ABV genotypes worldwide. To the best of our knowledge, this is the first report of a natural case of PDD associated with ABV infection in Japan.
- Published
- 2011
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20. Detection of highly pathogenic avian influenza virus infection in vaccinated chicken flocks by monitoring antibodies against non-structural protein 1 (NS1).
- Author
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Takeyama N, Minari K, Kajihara M, Isoda N, Sakamoto R, Sasaki T, Kokumai N, Takikawa N, Shiraishi R, Mase M, Hagiwara J, Kodama T, Imamura T, Sakaguchi M, Ohgitani T, Sawata A, Okamatsu M, Muramatsu M, Tsukamoto K, Lin Z, Tuchiya K, Sakoda Y, and Kida H
- Subjects
- Animals, Chickens, Enzyme-Linked Immunosorbent Assay veterinary, Influenza A virus immunology, Influenza in Birds virology, Poultry Diseases virology, Antibodies, Viral blood, Influenza Vaccines immunology, Influenza in Birds diagnosis, Influenza in Birds immunology, Poultry Diseases diagnosis, Poultry Diseases immunology, Viral Nonstructural Proteins immunology
- Abstract
H5 and H7 highly pathogenic avian influenza virus (HPAIV) represent a major global concern in poultries and human health. Avian influenza (AI) vaccines are available but not preferred for field applications, primarily because vaccination interferes with sero-surveillances of AIV infection. To overcome the problem, ELISA systems using non-structural protein 1 (NS1) of AIV as antigens (NS1-ELISA) have been developed to measure anti-NS1 antibodies that are raised in AIV-infected but not in vaccinated chickens. However, some AI-vaccinated chickens having a weak anti-virus immune response may subsequently be infected with AIV and spread the virus. This raises a concern for the validity of NS1-ELISA to detect AIV infection in previously vaccinated chickens. In this study, we developed NS1-ELISA and assessed its feasibility to detect HPAIV infection in chickens previously immunized with H5 or H7 AI vaccines. The results indicated that the NS1-ELISA could identify HPAIV infection in both unvaccinated and vaccinated chickens at 1 week after infection in correlation with results from time-consuming virus isolation tests. Taken together, the NS1-ELISA system would be valuable tool to define HPAIV infection when AI vaccine program is in place., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2011
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21. Prevalence of swine Torque teno virus genogroups 1 and 2 in Japanese swine with suspected post-weaning multisystemic wasting syndrome and porcine respiratory disease complex.
- Author
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Taira O, Ogawa H, Nagao A, Tuchiya K, Nunoya T, and Ueda S
- Subjects
- Animals, Circoviridae Infections epidemiology, Circoviridae Infections veterinary, Circoviridae Infections virology, DNA Virus Infections virology, DNA, Viral genetics, Japan epidemiology, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction veterinary, Porcine Postweaning Multisystemic Wasting Syndrome epidemiology, Respiration Disorders virology, Torque teno virus genetics, Torque teno virus isolation & purification, DNA Virus Infections veterinary, Porcine Postweaning Multisystemic Wasting Syndrome virology, Respiration Disorders veterinary, Sus scrofa, Torque teno virus classification
- Abstract
Torque teno virus (TTV) was first isolated from a human hepatitis patient in 1997. TTV was also identified in several animals, including pigs, cattle, sheep, cats and dogs. In this study, we analysed the prevalence of swine TTV genogroups 1 (TTV1) and 2 (TTV2) in Japanese swine populations with suspected post-weaning multisystemic wasting syndrome and porcine respiratory disease by using a nested polymerase chain reaction method. Of 153 serum samples from 16 different herds in Japan, TTV1 was detected in 46 samples (30%), TTV2 in 47 samples (31%) and both in 15 samples (10%). There was no significant difference in the detection rate among geographical regions. The overall prevalence rate of TTV genogroups was significantly lower in < or = 30-day-old pigs (11%) compared to that in older age groups (54-82%). These results suggest that swine TTV may be widespread in post-weaning pigs and could play aetiological roles in pig diseases in Japan. This is the first report on the prevalence of swine TTV in Japan.
- Published
- 2009
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22. Development of quantitative real-time polymerase chain reaction for detection of and discrimination between Erysipelothrix rhusiopathiae and other Erysipelothrix species.
- Author
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To H, Koyama T, Nagai S, Tuchiya K, and Nunoya T
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- Animals, DNA, Bacterial genetics, Erysipelothrix drug effects, Erysipelothrix genetics, Erysipelothrix Infections diagnosis, Gene Amplification, Joints microbiology, Mice, Polymerase Chain Reaction methods, Polymerase Chain Reaction veterinary, RNA, Bacterial genetics, RNA, Bacterial isolation & purification, RNA, Ribosomal, 16S genetics, RNA, Ribosomal, 16S isolation & purification, Reference Values, Spleen microbiology, Swine, Swine Diseases microbiology, Erysipelothrix isolation & purification, Reverse Transcriptase Polymerase Chain Reaction methods
- Abstract
Quantitative real-time polymerase chain reaction (qPCR) assays were developed and validated in combination with enrichment culture for the detection and discrimination of Erysipelothrix rhusiopathiae and other Erysipelothrix species from tissue samples. The targets for SYBR green qPCR assays were the 16S ribosomal RNA gene for Erysipelothrix species and a gene involved in capsular formation for E. rhusiopathiae. The specificity of the assays was assessed with Erysipelothrix species and other related bacterial species. The limit of detection was found to be 5 colony-forming units per reaction. Amplification of DNA extracted from spleen and joint samples spiked with increasing quantities of Erysipelothrix cells was shown to be equally sensitive to DNA extracted from a pure bacterial culture. The assays were evaluated with 88 tissue samples from 3 experimentally infected pigs and 50 mice and with 36 tissue samples from 3 naturally infected pigs and 11 noninfected pigs. Results were compared with those of direct qPCR and conventional culture. The qPCR after enrichment increased the diagnostic sensitivity over that of culture and qPCR, thereby significantly reducing the total time taken for the detection of E. rhusiopathiae and other Erysipelothrix species. Therefore, this technique could be used for practical applications.
- Published
- 2009
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23. Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and RNA viruses in pigs with multiple infections.
- Author
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Ogawa H, Taira O, Hirai T, Takeuchi H, Nagao A, Ishikawa Y, Tuchiya K, Nunoya T, and Ueda S
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- Animals, Sensitivity and Specificity, Swine, Swine Diseases virology, Virus Diseases diagnosis, DNA Viruses isolation & purification, Polymerase Chain Reaction methods, RNA Viruses isolation & purification, Reverse Transcriptase Polymerase Chain Reaction methods, Swine Diseases diagnosis, Virus Diseases veterinary
- Abstract
Multiplex PCR and multiplex RT-PCR were developed to identify nine viruses in pigs with multiple infections. These viruses are: porcine circovirus type 2 (PCV2), suid herpesvirus 1, porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), Japanese encephalitis virus, porcine rotavirus A (PoRV-A), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and Getah virus. These methods were shown to be high specificity and sensitivity. In the clinical application, a total of 75 field samples were examined by our methods and previously reported methods for PCV2, PRRSV, TGEV, and PEDV. As a result, the detection rates of our multiplex PCR and multiplex RT-PCR were higher than those of the previously reported methods. Furthermore, it was confirmed that 24 PCV2 positive samples were co-infected with other viruses, 11 with PRRSV, 10 with PPV, 2 with PoRV-A, and 1 with TGEV by a combination of multiplex PCR and multiplex RT-PCR. PPV and PoRV-A were newly detected by multiplex PCR and multiplex RT-PCR. These results suggest that the combination of our multiplex PCR and multiplex RT-PCR is useful for rapid and accurate identification of nine major pathogenic viruses in pigs with multiple infections.
- Published
- 2009
- Full Text
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24. [Epithelioid hemangioendothelioma of the liver diagnosed by laparoscopy guided biopsy].
- Author
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Komatsu N, Kurosaki M, Sato M, Tanaka T, Hirayama I, Yasui Y, Umeda N, Hosokawa T, Ueda K, Tuchiya K, Nakanishi H, Itakura J, Asahina Y, Akasaka H, Matsunaga K, Taki K, Kojiro M, and Izumi N
- Subjects
- Adult, Biopsy methods, Diagnosis, Differential, Diagnostic Imaging, Female, Humans, Liver pathology, Hemangioendothelioma, Epithelioid diagnosis, Hemangioendothelioma, Epithelioid pathology, Laparoscopy, Liver Neoplasms diagnosis, Liver Neoplasms pathology
- Abstract
A 35-year-old woman was admitted to our hospital for right upper quadrant pain and multiple liver tumor were detected by diagnostic imaging. Tumors located near the surface of the liver which accompanied capsular retraction. Diagnostic laparoscopy showed multiple white tone tumors with retraction of the adjacent liver capsule. Tumor targeted biopsy was performed. The pathologic diagnosis of epithelioid hemangioendothelioma (EHE) was made by the positive staining of factor VIII-related antigen. EHE tend to locate in peripheral and extend to the liver capsule. Therefore, we face difficulties in getting biopsy sample safely. Here we report a useful case of laparoscopic examination and biopsy in the diagnosis of EHE.
- Published
- 2008
25. A vaccine prepared from a non-pathogenic H7N7 virus isolated from natural reservoir conferred protective immunity against the challenge with lethal dose of highly pathogenic avian influenza virus in chickens.
- Author
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Sakabe S, Sakoda Y, Haraguchi Y, Isoda N, Soda K, Takakuwa H, Saijo K, Sawata A, Kume K, Hagiwara J, Tuchiya K, Lin Z, Sakamoto R, Imamura T, Sasaki T, Kokumai N, Kawaoka Y, and Kida H
- Subjects
- Animals, Antibodies, Viral analysis, Antibodies, Viral biosynthesis, Antibody Specificity, Chickens, Cross Reactions, DNA, Viral genetics, Disease Outbreaks prevention & control, Ducks immunology, Influenza A Virus, H7N7 Subtype immunology, Influenza Vaccines genetics, Influenza Vaccines immunology, Influenza in Birds genetics, Influenza in Birds immunology, Reassortant Viruses growth & development, Ducks virology, Influenza A Virus, H7N7 Subtype physiology, Influenza Vaccines administration & dosage, Influenza in Birds prevention & control, Reassortant Viruses immunology
- Abstract
During 2001-2004, 41 H7 influenza viruses (2 H7N1 and 39 H7N7 strains) were isolated from fecal samples of migratory ducks that flew from Siberia in the autumn of each year to Japan and Mongolia. A phylogenetic analysis of the hemagglutinin (HA) genes of the nine representative isolates revealed that they belonged to the Eurasian lineage and the deduced amino acid sequence at the cleavage site of the HAs represented apathogenic profiles. One of the H7 isolates A/duck/Mongolia/736/02 (H7N7) was chosen from these H7 isolates for the preparation of the test vaccine. To improve the growth potential of A/duck/Mongolia/736/02 (H7N7) in chicken embryos, A/duck/Hokkaido/Vac-2/04 (H7N7) was generated by genetic reassortment between A/duck/Mongolia/736/02 (H7N7) as the donor of the PB2, PB1, PA, HA, NA, and NS genes and A/duck/Hokkaido/49/98 (H9N2) as that of NP and M genes. The test vaccine was prepared as follows; A/duck/Hokkaido/Vac-2/04 (H7N7) was propagated in chicken embryos and the virus in the allantoic fluid was inactivated and adjuvanted to form an oil-in-water emulsion. The test vaccine conferred immunity to chickens, completely protecting the manifestation of clinical signs against the challenge with lethal dose of H7 highly pathogenic avian influenza virus. These results indicate that influenza viruses isolated from natural reservoirs are useful for vaccine strains.
- Published
- 2008
- Full Text
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26. Protection of mice from rabies by intranasal immunization with inactivated rabies virus.
- Author
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Yoneda A, Tuchiya K, Takashima Y, Arakawa T, Tsuji N, Hayashi Y, and Matsumoto Y
- Subjects
- Administration, Intranasal, Animals, Antibodies, Viral blood, Cholera Toxin administration & dosage, Enzyme-Linked Immunosorbent Assay, Female, Immunoglobulin G blood, Injections, Intraperitoneal, Mice, Inbred BALB C, Nasal Mucosa, Vaccines, Inactivated administration & dosage, Immunization methods, Mice, Rabies prevention & control, Rabies Vaccines administration & dosage, Rodent Diseases prevention & control
- Abstract
The mucosal immunization method is a needle-free alternative way of vaccination. This study evaluated the efficacy of mucosal immunization for rabies. Mice were intranasally administered five times with inactivated and concentrated rabies virus antigen (CRV) supplemented with or without cholera toxin (CT). The anti-rabies virus antibody titer of mice intranasally immunized with CRV plus CT (CRV/CT) was comparable to that of mice intraperitoneally immunized twice with the same amount of CRV. Virus neutralizing (VNA) titers of mice immunized intranasally with CRV/CT were slightly lower than those of intraperitoneally immunized mice. Both anti-rabies virus ELISA antibody and VNA titers of mice immunized with CRV without CT were significantly lower than those of mice immunized with CRV/CT. In mice intranasally immunized with CRV/CT, and intraperitoneally immunized mice, high levels of IgG(2a) antibody were detected, suggesting the activation of Th1-driven cellular immunity by the two ways of immunization. All immunized mice were challenged intracerebrally with a lethal dose of virulent rabies virus CVS strain. The survival rates of mice immunized with CRV/CT and CRV without CT were 67% and 17%, respectively, while the rate of intraperitoneally immunized mice was 100%. Antigen-specific whole IgG and IgG(2a), and VNA titers of survived mice were significantly higher than those of dead mice at the challenge day. These data suggest the possibility of intranasal immunization with inactivated antigen as a rabies vaccination strategy and the importance of a mucosal adjuvant such as CT.
- Published
- 2008
- Full Text
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27. [Primary arterial switch operation for complete transposition of the great arteries (type I) of a neonate weighing 1,378 g].
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Yamamoto Y, Kobayashi J, Yashiro K, Yoda H, Tuchiya K, Oosawa K, Obana K, Ishida K, and Kaneko Y
- Subjects
- Cardiac Surgical Procedures methods, Female, Humans, Infant, Newborn, Infant, Very Low Birth Weight, Transposition of Great Vessels surgery
- Abstract
A 2-day-old female baby, delivered by emergent cesarean section at 35 weeks of gestational age with a birth weight of 1,378 g, was referred to our institute for intensive care of heart failure. By echocardiography and cardiac catheterization, the patient was diagnosed with isolated complete transposition of the great arteries. Primary arterial switch operation was performed at 13 days of age. No technical difficulty arose, imposed by the small size of cardiovascular structure. On the 5th postoperative day, surgical repair of intestinal perforation was performed. Convalescence thereafter was uneventful. She returned home on the 64th postoperative day with the body weight of 2,310 g. We conclude that primary arterial switch operation can be a feasible surgical option even in a neonate with very low birth weight.
- Published
- 2008
28. Influence of vascularized transplant bed on fat grafting.
- Author
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Yazawa M, Mori T, Tuchiya K, Nakayama Y, Ogata H, and Nakajima T
- Subjects
- Animals, Coated Materials, Biocompatible, Graft Survival, Immunoenzyme Techniques, Necrosis, Neovascularization, Physiologic, Rabbits, Silicones, Transplantation, Autologous, Adipose Tissue transplantation, Fibroblast Growth Factor 2 pharmacology
- Abstract
Recent advances in regenerative medicine have opened up the option of materials used for transplantation. However, only a few studies have examined the take of transplanted tissues. We attempted to establish a functional bed for transplanted tissues using growth factors. A cylinder-type silicone substrate (spacer) was coated with a photoreactive gelatin containing basic fibroblast growth factor. This spacer was transplanted into the dorsal subdermal layer in a rabbit. After 2 and 4 weeks, the capsule formed around the spacer was histologically assessed for use as a transplant bed. In addition, after 2-4 weeks of spacer grafting, autologous fat was transplanted into the capsule. After 4 more weeks, the grafted fat was assessed immunohistochemically to evaluate the capsule as a functional bed for transplantation. In the groups pretreated with growth factors, proliferation of blood vessels was observed in the capsules. After fat grafting, a pattern of overall necrosis was observed in controls. However, good proliferation of blood vessels and favorable fat take were observed in the groups pretreated with growth factors. Necrosis, however, was found at the center of the grafted fat. We conclude that a vascularized transplant bed was useful for promoting take of the grafted fat.
- Published
- 2006
- Full Text
- View/download PDF
29. Seroepidemiological evidence for the possible presence of Babesia (Theileria) equi and Babesia caballi infections in donkeys in western Xinjiang, China.
- Author
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Chahan B, Zhang S, Seo JY, Nakamura C, Zhang G, Bannai H, Jian Z, Inokuma H, Tuchiya K, Sato Y, Kabeya H, Maruyama S, Mikami T, and Xuan X
- Subjects
- Animals, Babesiosis blood, Babesiosis epidemiology, Babesiosis parasitology, China epidemiology, Prevalence, Seroepidemiologic Studies, Babesiosis veterinary, Equidae blood, Equidae parasitology
- Abstract
The prevalence of Babesia (Theileria) equi and B. caballi infections in donkeys in western Xinjiang China was investigated. In total, 93 serum samples were randomly taken from donkeys in the Kashi and Ili areas, and examined for B. equi and B. caballi infections by enzyme-linked immunosorbent assays using recombinant antigens. Of the 93 samples, 9 (9.6%) and 36 (38.7%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 2 (2.2%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine babesiosis might be extensively prevalent in donkeys in western Xinjiang.
- Published
- 2006
- Full Text
- View/download PDF
30. Serological evidence of infection of Anaplasma and Ehrlichia in domestic animals in Xinjiang Uygur Autonomous Region area, China.
- Author
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Chahan B, Jian Z, Xuan X, Sato Y, Kabeya H, Tuchiya K, Itamoto K, Okuda M, Mikami T, Maruyama S, and Inokuma H
- Subjects
- Anaplasmosis microbiology, Animals, Antibodies, Bacterial blood, China epidemiology, Ehrlichiosis epidemiology, Ehrlichiosis microbiology, Fluorescent Antibody Technique, Indirect veterinary, Seroepidemiologic Studies, Anaplasma phagocytophilum isolation & purification, Anaplasmosis epidemiology, Animals, Domestic microbiology, Ehrlichia chaffeensis isolation & purification, Ehrlichiosis veterinary
- Abstract
Serological methods were utilized to detect Anaplasma and Ehrlichia infection in domestic animals in Xinjiang Uygur Autonomous Region, China. By using an indirect immunofluorescence assay (IFA), antibodies that reacted with Anaplasmaphagocytophilum and Ehrlichiachaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia.
- Published
- 2005
- Full Text
- View/download PDF
31. VP2 gene of a canine parvovirus isolate from stool of a puppy.
- Author
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Hirayama K, Kano R, Hosokawa-Kanai T, Tuchiya K, Tsuyama S, Nakamura Y, Sasaki Y, and Hasegawa A
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Dogs, Feces virology, Genes, Viral, Male, Molecular Sequence Data, Parvoviridae Infections virology, Phylogeny, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Capsid Proteins chemistry, Dog Diseases virology, Parvoviridae Infections veterinary, Parvovirus genetics
- Abstract
VP2 gene of a canine parvovirus (CPV) isolate from the feces of a puppy which was diagnosed to be CPV infection was analysed. The result indicated that this clinical isolate was phylogenetically close to the isolate of wild-type CPV (strain CPV-T37) prevailing in Taiwan rather than isolates from Japan.
- Published
- 2005
- Full Text
- View/download PDF
32. High level activity of 2', 5'-oligoadenylate synthetase in dog serum.
- Author
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Iwata A, Yamamoto A, Fujino M, Sato I, Hosokawa-Kanai T, Tuchiya K, Ishihama A, and Sokawa Y
- Subjects
- Animals, Antibodies, Monoclonal, Blotting, Western veterinary, Cats, Chromatography, DEAE-Cellulose veterinary, Female, Guinea Pigs, Rabbits, Radioimmunoassay veterinary, 2',5'-Oligoadenylate Synthetase blood, Dogs blood
- Abstract
Most animal cells that are exposed to interferon (IFN) experience an increase in the activity of 2', 5'-oligoadenylate synthetase (OAS), which is an important effector of IFN's antiviral action. OAS activity has been widely used in clinical chemistry as an indicator of IFN activity. In this study, we found that OAS activity in canine serum is 46.0 +/- 40.4 nmol/dl/hr, which is 10- to 100-fold higher than in other animals such as the cat (1.9 +/- 2.1), rabbit (4.0 +/- 1.1), and guinea pig (0.3 +/- 0.6). The canine OAS protein was detected by Western blotting using a 68M-10 monoclonal anti-murine OAS antibody, and was found to be composed of at least three distinct molecular species of p40 class OAS. Among these, the 40 and 42 kDa components were determined to be the major species in serum and fibroblast cell lines, respectively.
- Published
- 2004
- Full Text
- View/download PDF
33. Molecular analysis of congenital central hypoventilation syndrome.
- Author
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Sasaki A, Kanai M, Kijima K, Akaba K, Hashimoto M, Hasegawa H, Otaki S, Koizumi T, Kusuda S, Ogawa Y, Tuchiya K, Yamamoto W, Nakamura T, and Hayasaka K
- Subjects
- Age of Onset, Amino Acid Substitution, Child, Child, Preschool, DNA Mutational Analysis, Female, Glial Cell Line-Derived Neurotrophic Factor, Glial Cell Line-Derived Neurotrophic Factor Receptors, Humans, Infant, Male, Nerve Growth Factors genetics, Polymorphism, Single Nucleotide, Proto-Oncogene Proteins genetics, Proto-Oncogene Proteins c-ret, Receptor Protein-Tyrosine Kinases genetics, Respiration, Artificial, Syndrome, Sleep Apnea, Central genetics
- Abstract
Congenital central hypoventilation syndrome (CCHS or Ondine's curse; OMIM 209880) is a disorder characterized by an idiopathic failure of the automatic control of breathing. CCHS is frequently complicated with neurocristopathies such as Hirschsprung's disease (HSCR). The genes involved in the RET-GDNF signaling and/or EDN3-EDNRB signaling pathways have been analyzed as candidates for CCHS; however, only a few patients have mutations of the RET, EDN3, and GDNF genes. Recently, mutations of the PHOX2B gene, especially polyalanine expansions, have been detected in two thirds of patients. We studied the RET, GDNF, GFRA1, PHOX2A, PHOX2B, HASH-1, EDN1, EDN3, EDNRB, and BDNF genes in seven patients with isolated CCHS and three patients with HSCR. We detected polyalanine expansions and a novel frameshift mutation of the PHOX2B gene in four patients and one patient, respectively. We also found several mutations of the RET, GFRA1, PHOX2A, and HASH-1 genes in patients with or without mutations of the PHOX2B gene. Our study confirmed the prominent role of mutations in the PHOX2B gene in the pathogenesis of CCHS. Mutations of the RET, GFRA1, PHOX2A, and HASH-1 genes may also be involved in the pathogenesis of CCHS. To make clear the pathogenesis of CCHS, the analysis of more cases and further candidates concerned with the development of the autonomic nervous system is required.
- Published
- 2003
- Full Text
- View/download PDF
34. Absence of superoxide dismutase activity in a soluble cellular isoform of prion protein produced by baculovirus expression system.
- Author
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Sakudo A, Hamaishi M, Hosokawa-Kanai T, Tuchiya K, Nishimura T, Saeki K, Matsumoto Y, Ueda S, and Onodera T
- Subjects
- Animals, Gene Expression, Genetic Vectors, Mice, Protein Engineering methods, Protein Isoforms genetics, Protein Isoforms metabolism, Recombinant Fusion Proteins analysis, Recombinant Fusion Proteins isolation & purification, Recombinant Fusion Proteins metabolism, Spodoptera genetics, Baculoviridae genetics, PrPC Proteins genetics, PrPC Proteins metabolism, Superoxide Dismutase metabolism
- Abstract
A method for expression and purification of a soluble form of histidine (HIS)-tagged murine prion protein (bacMuPrP), which lacks the entire C-terminal cleavage and glycosyl phosphatidyl inositol (GPI) addition site, has been developed using a recombinant baculovirus expression system and purification with Ni-NTA agarose affinity chromatography. In mammalian sources, PrP(C) is attached to the cell membrane by a GPI anchor. However, in our system, bacMuPrP was secreted into the media, enabling its easy purification in abundance. Indirect immunofluorescence studies and immunoblot analysis localized not in cell membrane but in the perinuclear endoplasmic reticulum region in cells and is secreted into the media. Tunicamycin treatment revealed non-glycosylated proteins were secreted into the media, suggesting that glycosylation is not necessary for bacMuPrP secretion. Density-gradient sedimentation analysis demonstrated a sedimentation coefficient of secretory bacMuPrP as 2.3 S, indicating a monomeric form. Although affinity-purified PrP from mouse brain or recombinant prion protein (PrP) produced by Escherichia coli and refolded in the presence of copper has been reported to display superoxide dismutase (SOD) activity, bacMuPrP did not show SOD activity. These results suggest that bacMuPrP has a different biochemical and biophysical characterization from mammalian and bacterial-derived PrP. Furthermore, this simple expression system may provide an adequate source for structural, functional, and biochemical analyses of PrP.
- Published
- 2003
- Full Text
- View/download PDF
35. Clinical study of a spacer to help prevent osteoradionecrosis resulting from brachytherapy for tongue cancer.
- Author
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Obinata K, Ohmori K, Tuchiya K, Nishioka T, Shirato H, and Nakamura M
- Subjects
- Adult, Aged, Aged, 80 and over, Chi-Square Distribution, Female, Humans, Male, Mandible diagnostic imaging, Mandibular Diseases etiology, Middle Aged, Osteoradionecrosis etiology, Retrospective Studies, Tomography, X-Ray Computed, Brachytherapy adverse effects, Mandibular Diseases prevention & control, Osteoradionecrosis prevention & control, Protective Devices, Tongue Neoplasms radiotherapy
- Abstract
Objective: We sought to describe a simple method to construct a spacer and to evaluate with the use of computed tomography the spacer's effectiveness in preventing osteoradionecrosis of the mandible., Study Design: Fifty-three patients with oral tongue cancers who were treated by means of interstitial brachytherapy were included in this study. Patients underwent a computed tomography examination immediately after the implantation of radioactive sources, with the spacers in place. Distances between the radioactive sources and the lingual surfaces of the mandible were measured on transverse computed tomographs and were evaluated in terms of the development of osteoradionecrosis in the mandible., Results: Statistically significant differences in the frequency of osteoradionecrosis were observed between patients who had received spacers equal to or thicker than 5 mm and those who had received spacers less than 5 mm thick., Conclusion: A spacer should have a minimum thickness of 5 mm on its lingual flange to prevent the development of osteoradionecrosis of the mandible.
- Published
- 2003
- Full Text
- View/download PDF
36. Expression in Escherichia coli and purification of the functional feline granulocyte colony-stimulating factor.
- Author
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Yamamoto A, Iwata A, Saitoh T, Tuchiya K, Kanai T, Tsujimoto H, Hasegawa A, Ishihama A, and Ueda S
- Subjects
- Animals, Cats, Cell Division drug effects, Dose-Response Relationship, Drug, Gene Expression, Granulocyte Colony-Stimulating Factor biosynthesis, Granulocyte Colony-Stimulating Factor genetics, Granulocytes drug effects, Leukocyte Count veterinary, Macrophages drug effects, Neutrophils drug effects, Neutrophils immunology, Recombinant Proteins, Time Factors, Escherichia coli genetics, Granulocyte Colony-Stimulating Factor isolation & purification, Granulocyte Colony-Stimulating Factor pharmacology
- Abstract
Feline granulocyte colony-stimulating factor (G-CSF) with an N-terminal histidine hexamer tag was expressed as inclusion bodies in E. coli. The G-CSF solubilized in 6 M guanidine solution was absorbed onto a Ni-NTA column and, after washing with decreasing concentrations of guanidine, eluted with imidazole in a soluble and apparently pure form. The activity of the recombinant feline G-CSF was 3 x 10(6)U/mg protein, as assayed by its stimulatory effect on NFS-60 cell proliferation. When a low level of purified feline G-CSF was administered once a day for two successive days to cats, the number of neutrophil increased 4-fold while the levels of other blood cell types remained virtually unchanged. Daily administration of G-CSF for a total of 11 days led to a more than 10-fold increase in neutrophils, an 8-fold increase in the number of monocytes and 2-fold increase in lymphocytes. No severe side effects or antibody production was observed in cats after administration of G-CSF., (Copyright 2002 Elsevier Science B.V.)
- Published
- 2002
- Full Text
- View/download PDF
37. [MR imaging of a case of spontaneous carotid-cavernous fistula].
- Author
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Tuchiya K, Koide R, Nakauchi J, Ide K, Sato M, Isozaki E, and Hirai S
- Subjects
- Aged, Female, Humans, Carotid-Cavernous Sinus Fistula diagnosis, Imaging, Three-Dimensional, Magnetic Resonance Imaging methods
- Published
- 2002
38. [A case of acute-onset autoimmune hepatitis with rheumatoid arthritis].
- Author
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Morita Y, Tuchiya K, Sato N, Ishikura T, Ishii K, Nomura T, Ariake K, Suzuki S, Sakurazawa T, Horiuchi T, Shimoi K, Takashimizu I, Ohkusa T, and Watanabe M
- Subjects
- Acute Disease, Adult, Chronic Disease, Humans, Male, Arthritis, Rheumatoid complications, Hepatitis, Autoimmune complications
- Published
- 2000
39. Biological and immunogenic properties of rabies virus glycoprotein expressed by canine herpesvirus vector.
- Author
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Xuan X, Tuchiya K, Sato I, Nishikawa Y, Onoderaz Y, Takashima Y, Yamamoto A, Katsumata A, Iwata A, Ueda S, Mikami T, and Otsuka H
- Subjects
- Animals, Antibodies, Monoclonal, Antigens, Viral genetics, Cell Line, Dog Diseases immunology, Dog Diseases prevention & control, Dogs, Gene Expression, Genes, Viral, Humans, Rabies immunology, Rabies prevention & control, Rabies veterinary, Rabies Vaccines genetics, Rabies Vaccines immunology, Recombinant Proteins genetics, Recombinant Proteins immunology, Recombination, Genetic, Vaccines, Synthetic genetics, Vaccines, Synthetic immunology, Genetic Vectors, Glycoproteins genetics, Glycoproteins immunology, Herpesvirus 1, Canid genetics, Rabies virus genetics, Rabies virus immunology, Viral Envelope Proteins genetics, Viral Envelope Proteins immunology
- Abstract
In order to evaluate whether canine herpesvirus (CHV) could be used as a live vector for the expression of heterologous immunogenes, we constructed a recombinant canine herpesvirus (CHV) expressing glycoprotein (G protein) of rabies virus (RV). The gene of G protein was inserted within the thymidine kinase gene of CHV YP11mu strain under the control of the human cytomegalovirus immediate early promoter. The G protein expressed by the recombinant CHV was processed and transported to the cell surface as in RV infected cells, and showed the same biological activities such as low pH dependent cell fusion and hemadsorption. The antigenic authenticity of the recombinant G protein was confirmed by a panel of monoclonal antibodies specific for G protein. Dogs inoculated intransally with the recombinant CHV produced higher titres of virus neutralizing antibodies against RV than those inoculated with a commercial, inactivated rabies vaccine. These results suggest that the CHV recombinant expressing G protein can be used as a vaccine to control canine rabies and that CHV may be useful as a vector to develop live recombinant against other infectious diseases in dogs.
- Published
- 1998
- Full Text
- View/download PDF
40. [An autopsied case of purulent meningitis associated with ocular flutter].
- Author
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Orimo S, Sato H, Ozawa E, Yasui H, and Tuchiya K
- Subjects
- Aged, Brain Stem pathology, Female, Humans, Meningitis, Bacterial microbiology, Meningitis, Bacterial pathology, Streptococcus pneumoniae, Meningitis, Bacterial complications, Ocular Motility Disorders etiology, Streptococcal Infections
- Abstract
We report a 72-year-old autopsied case of purulent meningitis associated with ocular flutter. She was admitted to our hospital because of disturbances of consciousness and fever. Physical examination revealed fever, tachycardia, and tachypnea. Neurological examination showed disturbance of consciousness (Japan Coma Scale 30), agitated state, anisocoria, sluggish and fixed reaction of pupils to light, and nuchal stiffness. Routine blood examination showed leukocytosis, thrombocytopenia, positive CRP, and elevated myocardial enzymes. Cerebrospinal fluid revealed pleocytosis with predominant leukocytes, elevated protein, and decreased glucose (22% of blood glucose), and Streptococcus pneumoniae was proved in culture. Brain CT scan revealed no abnormal findings. Electrocardiography showed tachycardia, left axis deviation, and elevated ST segment in aVF, and V3-V6. Ultrasonic echocardiography revealed slight hypokinesis of the left anterior wall, septum, and apex. She was diagnosed as having purulent meningitis, myocarditis, probable encephalitis. Thus, antibiotics, acycrovir, glycerol, and aspirin were administrated. But her respiration deteriorated and ocular flutter was observed for 15 minutes. After that, She required artificial ventilation and eventually died after 29 hours the admission to our hospital. Pathological examination revealed leukocyte accumulation in the arachnoid space of the derebral surface, especially frontal and parietal lobes. Uncal herniation was not observed. The brainstem and cerebellum were histologically within normal limits. These findings suggest that ocular flutter observed in this patient was caused by functional damage of the brainstem.
- Published
- 1998
41. Development of an enzyme-linked immunosorbent assay using recombinant chicken anemia virus proteins expressed in a baculovirus vector system.
- Author
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Iwata N, Fujino M, Tuchiya K, Iwata A, Otaki Y, and Ueda S
- Subjects
- Animals, Antibody Formation, Antigens, Viral biosynthesis, Antigens, Viral immunology, Baculoviridae, Blotting, Western, Cell Line, Chicken anemia virus immunology, Chickens, Circoviridae Infections diagnosis, Circoviridae Infections immunology, Enzyme-Linked Immunosorbent Assay methods, Japan, Polymerase Chain Reaction, Recombinant Proteins analysis, Recombinant Proteins biosynthesis, Recombinant Proteins immunology, Spodoptera, T-Lymphocytes, Transfection, Viral Proteins biosynthesis, Viral Proteins immunology, Antibodies, Viral biosynthesis, Antigens, Viral analysis, Chicken anemia virus isolation & purification, Circoviridae Infections veterinary, Poultry Diseases, Viral Proteins analysis
- Abstract
Recombinant baculoviruses were constructed to express the putative proteins VP1, VP2 or VP3 of the chicken anemia virus (CAV). The recombinant VP1, VP2 or VP3 were detected by SDS-PAGE, and their molecular weights were 50, 30/27 and 16 kDa, respectively. The VP2 and VP3 reacted with sera from CAV-infected chickens in Western blot analysis and when used as an enzyme-linked immunosorbent assay (ELISA) antigen, but VP1 did not. Antibodies to CAV were detected, by ELISA using crude insect cell lysates containing VP2 or VP3, from 2 to 20 weeks or 2 to 7 weeks after CAV infection, respectively. These findings indicate that recombinant VP2 and VP3 expressed in the baculovirus vector system can be used as antigens to detect anti-CAV antibodies in ELISA.
- Published
- 1998
- Full Text
- View/download PDF
42. Construction of canine herpesvirus vector expressing foreign genes using a lacZ-TK gene cassette as a double selectional marker.
- Author
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Xuan X, Nishikawa Y, Takashima Y, Tuchiya K, Ueda S, Yokoyama N, Maeda K, Mikami T, and Otsuka H
- Subjects
- Animals, Biomarkers, Cell Line, Cloning, Molecular, DNA, Viral analysis, DNA, Viral genetics, Dogs, Gene Expression Regulation, Viral, Recombinant Fusion Proteins analysis, Recombinant Fusion Proteins genetics, Genetic Vectors genetics, Herpesvirus 1, Canid genetics, Lac Operon genetics, Thymidine Kinase genetics, Transgenes genetics
- Abstract
An improved method for constructing canine herpesvirus (CHV) recombinants expressing foreign genes by using the lacZ-TK gene cassette as a double selectional marker was developed. A recombinant CHV carrying the lacZ-TK gene at a targeted gene locus was constructed and used as a parental virus for generating new recombinants. The parental virus formed blue plaques and was sensitive to TK-specific drugs, while newly generated recombinants, in which the lacZ-TK gene was replaced with the desired foreign gene, become both resistant to the TK-specific drugs and formed white plaques. Recombinants were isolated by using the combination of drug selection and color selection. This improved method allows construction of recombinant CHV with great ease, because the drug selection can enrich the frequency of recombinant CHV from 0.01-0.1% to 10-80%. This method was employed to construct a recombinant CHV that expressed rabies virus (RV) glycoprotein (G protein).
- Published
- 1998
- Full Text
- View/download PDF
43. Cloning of the enomycin structural gene from Streptomyces mauvecolor and production of recombinant enomycin in Escherichia coli.
- Author
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Takeuchi S, Oka T, Sakata N, S-Tuchiya K, Hayashi H, and Hori M
- Subjects
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli genetics, Molecular Sequence Data, Anti-Bacterial Agents metabolism, Antibiotics, Antineoplastic metabolism, Genes, Bacterial, Peptides, Recombinant Proteins biosynthesis, Streptomyces genetics
- Abstract
A genomic DNA library from the enomycin (ENM) producer, Streptomyces mauvecolor, was screened for the ENM structural gene (enm) by use of a segment of the phenomycin gene (phm) as the probe, and a plasmid, pENI, was constructed. By primer-walking along the insert, a 573 bp DNA sequence that contain an ORF corresponding to preENM was determined. The deduced amino acid composition of ENM was close to that previously reported (MIZUNO, S.; K. NITTA & H. UMEZAWA: Mode of action of enomycin, an antitumor antibiotic of high molecular weight. I. Inhibition of protein synthesis. J. Biochem. 61: 373 approximately 381, 1967). The producer cells expressed enm during an ENM-productive fermentation. An enm-expression plasmid, pENE 1, was constructed, with which E. coli AD202 was transformed. The transformant produced a fusion protein consisting of glutathione-S-transferase (GST) and ENM. The genetically engineered ENM (rENM) inhibited the growth of Hela cells in vitro. Comparison of the base sequence spanning enm with that spanning phm showed that the structural genes were conserved more extensively than were the flanking regions, though the genes were unlikely to be essential to the lives of the producers.
- Published
- 1997
- Full Text
- View/download PDF
44. Prostaglandin E2 increases the calcium concentration in rat brown adipocytes and their consumption of oxygen.
- Author
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Nagai M, Tuchiya K, and Kojima H
- Subjects
- Adipose Tissue, Brown cytology, Adipose Tissue, Brown drug effects, Animals, Cytosol drug effects, Cytosol metabolism, Male, Oxygen Consumption drug effects, Rats, Rats, Wistar, Adipose Tissue, Brown metabolism, Calcium metabolism, Dinoprostone pharmacology, Oxygen Consumption physiology
- Abstract
Effects of prostaglandin E2 (PGE2) were examined on the oxygen consumption and intracellular calcium concentration of rat brown adipose tissue (BAT). PGE2 0.1 nM-1 microM increased oxygen consumption of the tissue blocks of BAT, with a maximum 2-13 min after PGE2 administration. PGE2 was most effective at 1 and 10 nM, and the oxygen consumption was elevated for over 40 min. Pretreatment of BAT with indomethacin, a prostaglandin synthesis inhibitor, did not affect the increase in oxygen consumption induced by noradrenaline. PGE2 at 1-10 nM gradually increased the intracellular calcium concentration of freshly dispersed single brown adipocytes by 3-4 times in 30 min. PGF2 also increased the intracellular calcium concentration of brown adipocytes in calcium-free medium. These results raise the possibility that PGE2 and noradrenaline affect heat genesis and metabolism of BAT independently.
- Published
- 1996
- Full Text
- View/download PDF
45. Characterization of pseudorabies virus glycoprotein gII expressed by recombinant baculovirus.
- Author
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Xuan X, Nakamura T, Ihara T, Sato I, Tuchiya K, Nosetto E, Ishihama A, and Ueda S
- Subjects
- Amino Acid Sequence, Animals, Antibodies, Monoclonal immunology, Antibodies, Viral immunology, Antigens, Viral immunology, Base Sequence, Cells, Cultured, Female, Gene Expression Regulation, Viral, Genetic Vectors, Herpesvirus 1, Suid immunology, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Neutralization Tests, Protein Biosynthesis, Protein Serine-Threonine Kinases, Proteins immunology, Pseudorabies prevention & control, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Recombinant Proteins immunology, Spodoptera, Vaccination, Vaccines, Synthetic immunology, Viral Vaccines immunology, Herpesvirus 1, Suid genetics, Nuclear Proteins, Nucleopolyhedroviruses genetics, Proteins genetics, Transfection
- Abstract
The gene encoding the complete glycoprotein gII (homologue of gB of herpes simplex virus) of pseudorabies virus (PrV) was inserted into a baculovirus transfer vector, and a recombinant virus expressing gII was isolated. Three gII-related recombinant baculovirus-expressed peptides of 100, 60, and 45 to 50 kDa were detected with a polyclonal antibody against gII; these correspond to the authentic subunits gIIa and its cleavage products gIIb and gIIc, respectively. These proteins were subjected to N-terminal sequencing, and the results showed that the protease cleavage sites were identical to those of authentic gII. The expressed gII was shown to be transported to the surface of infected cells as judged by an indirect immunofluorescence test. Antibodies raised in mice immunized with the recombinant gII neutralized the infection of PrV in vitro. Mice inoculated with the recombinant gII were completely protected from lethal challenge with PrV.
- Published
- 1995
- Full Text
- View/download PDF
46. Characterization of rabies virus glycoprotein expressed by recombinant baculovirus.
- Author
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Tuchiya K, Matsuura Y, Kawai A, Ishihama A, and Ueda S
- Subjects
- Animals, Baculoviridae genetics, Base Sequence, Cells, Cultured, Cloning, Molecular, DNA, Viral genetics, Genetic Vectors, Glycoproteins chemistry, Glycosylation, Molecular Sequence Data, Recombinant Proteins genetics, Viral Fusion Proteins chemistry, Glycoproteins genetics, Rabies virus genetics, Viral Fusion Proteins genetics
- Abstract
A cDNA of the glycoprotein (G protein) gene of rabies virus Nishigahara strain was cloned and inserted into a baculovirus genome under the control of the polyhedrin promoter. Infection of Spodoptera frugiperda cells with this recombinant virus produced a large quantity of new protein instead of the parental polyhedrin protein. By immunofluorescent and immunoblotting analyses, the recombinant protein was antigenically similar to the authentic G protein. Its molecular mass estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, however, was slightly smaller than that of the authentic one, and this observation was suggested to be due to the difference in glycosylation level between the two G proteins. The recombinant G protein expressed on the cell surface of the insect cells showed a fusion activity at low pH. The fusion activity was inhibited by antiserum against either whole virions or G protein of rabies virus.
- Published
- 1992
- Full Text
- View/download PDF
47. Reconstitution of influenza virus RNA polymerase from three subunits expressed using recombinant baculovirus system.
- Author
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Kobayashi M, Tuchiya K, Nagata K, and Ishihama A
- Subjects
- Animals, Cell Line, Chromatography, Gel, Cloning, Molecular, DNA-Directed RNA Polymerases isolation & purification, DNA-Directed RNA Polymerases metabolism, Electrophoresis, Polyacrylamide Gel, Recombinant Proteins metabolism, Baculoviridae genetics, DNA-Directed RNA Polymerases genetics, Orthomyxoviridae enzymology
- Abstract
Influenza virus RNA polymerase catalyzes multiple step reactions in transcription and replication of the genome RNA. The core enzyme is composed of each one of the three P proteins, PB1, PB2 and PA (Honda et al. (1990) J. Biochem. 107, 624-628). For detailed analysis of the role of each P protein and of the functional domains on each P polypeptide, we expressed individual P proteins in cultured insect cells after infection with recombinant baculoviruses. PB1 and PB2 accumulated in cell nuclei whereas PA stayed in cytoplasm. Both the PB1 and PB2 proteins were purified from aggregates in the respective nuclear extract, and the PA was partially purified from the cytoplasm. RNA polymerase was reconstituted by mixing the three P proteins in a urea solution and then dialyzing against a reconstitution buffer. The reconstituted enzyme was able to transcribe model RNA templates. Minus-sense RNA was a better template than plus-sense RNA.
- Published
- 1992
- Full Text
- View/download PDF
48. [Study on argyrophilic inclusions of multisystem atrophy (Oppenheimer)].
- Author
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Iwabuchi K, Kosaka K, Haga C, Tuchiya K, Amano N, Itoh K, Yagishita S, and Mizutani Y
- Subjects
- Alzheimer Disease pathology, Cerebellar Ataxia pathology, Humans, Olivopontocerebellar Atrophies genetics, Olivopontocerebellar Atrophies pathology, Pons pathology, Silver Staining, Spinocerebellar Degenerations genetics, Inclusion Bodies pathology, Spinocerebellar Degenerations pathology
- Abstract
Unlabelled: Non-hereditary olivo-ponto-cerebellar atrophy (OPCA) and striato-nigral degeneration (SND) have been looked upon as a single disease entity called multisystem atrophy (MSA) by Oppenheimer. This study revealed that both intracytoplasmic argyrophilic inclusions (AI) in pontine neurons and glial (argyrophilic) cytoplasmic inclusions (GCIs) widely distributed in the CNS are characteristics of MSA., Materials: a) 12 cases with MSA, b) 16 cases with autosomal dominant (AD) form of spinocerebellar degeneration (SCD): AD form of OPCA 5 cases, Joseph disease 4 cases, AD-dentatorubropallidoluysian atrophy (Naitoh & Oyanagi's form) 6 cases, AD-spastic ataxia (Brown) 1 case, c) 4 cases with autosomal recessive (AR) form of SCD: AR form of OPCA 1 case, myoclonic epilepsy with ragged-red fibers (MERRF) 1 case, complicated form of spastic paraplegia 2 cases, d) 6 cases with non-hereditary SCD including intoxications: late cortical cerebellar atrophy 1 case, alcoholic cerebellar degeneration 2 cases, phenytoin-induced cerebellar degeneration 1 case, neuroleptic malignant syndrome 1 case, and e) 27 cases with other neuropsychiatric diseases: Alzheimer disease 20 cases, progressive supranuclear palsy 5 cases, schizophrenia 2 cases., Method: We examined 10 mu-thick paraffin sections stained with HE, Klüver-Barrera, Bodian, Holzer, Gallyas, and Bielschowski methods., Results: AI in pontine neurons were found only in two cases of MSA. Interestingly no AI could be detected even in cases with AD form of OPCA showing mild degeneration in the pontocerebellar system. On the other hand, GCIs were found in all cases with MSA irrespective of the degree of degeneration in the olivo-ponto-cerebellar or striato-nigral system. However, there was no GCIs in cases with other form of SCD and other neuropsychiatric diseases. Gallyas stain was the best method for detecting GCIs. GCIs were widely distributed in the CNS except for superficial layers of the cerebral cortex, the cerebellar cortex, and the dorsal column of the spinal cord. There were also many GCIs in the putamen, pontine base, and cerebellar white matter, even though these sites were well preserved.
- Published
- 1991
49. Characterization of a feline infectious peritonitis virus isolate.
- Author
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Goitsuka R, Tsuji M, Ohashi T, Onda C, Tuchiya K, Hirota Y, Takahashi E, and Hasegawa A
- Subjects
- Animals, Antigens, Viral analysis, Cats, Cell Line, Coronaviridae immunology, Coronaviridae ultrastructure, Coronaviridae Infections microbiology, Cytopathogenic Effect, Viral, Female, Fluorescent Antibody Technique, Interleukin-6 biosynthesis, Microscopy, Electron, Peritonitis microbiology, Virion ultrastructure, Cat Diseases microbiology, Coronaviridae growth & development, Coronaviridae Infections veterinary, Peritonitis veterinary
- Published
- 1991
- Full Text
- View/download PDF
50. Enzyme-linked immunosorbent assay for the detection of canine coronavirus and its antibody in dogs.
- Author
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Tuchiya K, Horimoto T, Azetaka M, Takahashi E, and Konishi S
- Subjects
- Animals, Antigens, Viral analysis, Coronaviridae immunology, Coronaviridae Infections diagnosis, Dogs, Feces microbiology, Fluorescent Antibody Technique, Neutralization Tests, Predictive Value of Tests, Antibodies, Viral blood, Coronaviridae isolation & purification, Coronaviridae Infections veterinary, Dog Diseases diagnosis, Enzyme-Linked Immunosorbent Assay
- Abstract
Two methods of enzyme-linked immunosorbent assay (ELISA) were developed for the diagnosis of canine coronavirus (CCV) infection in dogs. One ELISA, in which CCV-infected CRFK cell lysate is used as antigen, is for the detection and titration of antibody against CCV, and the other ELISA uses the double antibody sandwich method for the detection of CCV antigen. The first ELISA procedure demonstrated antibody responses in dogs inoculated with CCV, as did the virus neutralization test; the second ELISA detected specific CCV antigen in feces and organ homogenates of inoculated dogs.
- Published
- 1991
- Full Text
- View/download PDF
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