12 results on '"Tsopoulidis N"'
Search Results
2. T cell receptor–triggered nuclear actin network formation drives CD4+ T cell effector functions.
- Author
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Tsopoulidis, N., Kaw, S., Laketa, V., Kutscheidt, S., Baarlink, C., Stolp, B., Grosse, R., and Fackler, O. T.
- Subjects
T cells ,IMMUNE response ,CELL physiology ,ACTIN ,T cell receptors - Abstract
T cells need nuclear F-actin: T cell activation is regulated by numerous mechanisms upon T cell antigen receptor (TCR) engagement, including induction of specific cytokines by transcription factors like NF-κB and NFAT. Tsopoulidis et al. now show that TCR engagement causes rapid nuclear actin polymerization to create a dynamic actin filament network that is critical to CD4
+ T cell effector functions. Nuclear actin filament polymerization involves the nuclear Arp2/3 complex that is induced by nuclear Ca2+ and regulated by N-Wasp and NIK. Specific inhibition of nuclear actin filament formation impairs T cell effector responses, including cytokine expression and CD4+ T cell help for antibody production. Together, these data reveal a role for nuclear actin filaments in driving CD4+ T cell effector functions. T cell antigen receptor (TCR) signaling triggers selective cytokine expression to drive T cell proliferation and differentiation required for immune defense and surveillance. The nuclear signaling events responsible for specificity in cytokine gene expression upon T cell activation are largely unknown. Here, we uncover formation of a dynamic actin filament network in the nucleus that regulates cytokine expression for effector functions of CD4+ T lymphocytes. TCR engagement triggers the rapid and transient formation of a nuclear actin filament network via nuclear Arp2/3 complex, induced by elevated nuclear Ca2+ levels and regulated via N-Wasp and NIK. Specific interference with TCR-induced formation of nuclear actin filaments impairs production of effector cytokines and prevents generation of antigen-specific antibodies but does not interfere with immune synapse formation and cell proliferation. Ca2+ -regulated actin polymerization in the nucleus allows CD4+ T cells the rapid conversion of TCR signals into effector functions required for T cell help. [ABSTRACT FROM AUTHOR]- Published
- 2019
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3. ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting.
- Author
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Tashev SA, Euchner J, Yserentant K, Hänselmann S, Hild F, Chmielewicz W, Hummert J, Schwörer F, Tsopoulidis N, Germer S, Saßmannshausen Z, Fackler OT, Klingmüller U, and Herten DP
- Subjects
- Humans, CD4-Positive T-Lymphocytes metabolism, Adaptor Proteins, Signal Transducing metabolism, Lymphocyte Activation, HIV-1, Staining and Labeling methods, Microscopy, Fluorescence methods
- Abstract
Determining the label to target ratio, also known as the degree of labeling (DOL), is crucial for quantitative fluorescence microscopy and a high DOL with minimal unspecific labeling is beneficial for fluorescence microscopy in general. Yet robust, versatile and easy-to-use tools for measuring cell-specific labeling efficiencies are not available. Here we present a DOL determination technique named protein-tag DOL (ProDOL), which enables fast quantification and optimization of protein-tag labeling. With ProDOL various factors affecting labeling efficiency, including substrate type, incubation time and concentration, as well as sample fixation and cell type can be easily assessed. We applied ProDOL to investigate how human immunodeficiency virus-1 pathogenesis factor Nef modulates CD4 T cell activation measuring total and activated copy numbers of the adapter protein SLP-76 in signaling microclusters. ProDOL proved to be a versatile and robust tool for labeling calibration, enabling determination of labeling efficiencies, optimization of strategies and quantification of protein stoichiometry., (© 2024. The Author(s).)
- Published
- 2024
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4. ARPC5 isoforms and their regulation by calcium-calmodulin-N-WASP drive distinct Arp2/3-dependent actin remodeling events in CD4 T cells.
- Author
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Sadhu L, Tsopoulidis N, Hasanuzzaman M, Laketa V, Way M, and Fackler OT
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- Humans, Actin-Related Protein 2 metabolism, Actin-Related Protein 2-3 Complex genetics, Actin-Related Protein 2-3 Complex metabolism, Calcium metabolism, CD4-Positive T-Lymphocytes metabolism, Protein Isoforms genetics, Protein Isoforms metabolism, Receptors, Antigen, T-Cell metabolism, Actins metabolism, Calmodulin metabolism
- Abstract
CD4 T cell activation induces nuclear and cytoplasmic actin polymerization via the Arp2/3 complex to activate cytokine expression and strengthen T cell receptor (TCR) signaling. Actin polymerization dynamics and filament morphology differ between nucleus and cytoplasm. However, it is unclear how the Arp2/3 complex mediates distinct nuclear and cytoplasmic actin polymerization in response to a common stimulus. In humans, the ARP3, ARPC1, and ARPC5 subunits of the Arp2/3 complex exist as two different isoforms, resulting in complexes with different properties. Here, we show that the Arp2/3 subunit isoforms ARPC5 and ARPC5L play a central role in coordinating distinct actin polymerization events in CD4 T cells. While ARPC5L is heterogeneously expressed in individual CD4 T cells, it specifically drives nuclear actin polymerization upon T cell activation. In contrast, ARPC5 is evenly expressed in CD4 T cell populations and is required for cytoplasmic actin dynamics. Interestingly, nuclear actin polymerization triggered by a different stimulus, DNA replication stress, specifically requires ARPC5 but not ARPC5L. TCR signaling but not DNA replication stress induces nuclear actin polymerization via nuclear calcium-calmodulin signaling and N-WASP. Diversity in the molecular properties and individual expression patterns of ARPC5 subunit isoforms thus tailors Arp2/3-mediated actin polymerization to different physiological stimuli., Competing Interests: LS, NT, MH, VL, MW, OF No competing interests declared, (© 2023, Sadhu et al.)
- Published
- 2023
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5. NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia.
- Author
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Witkowski MT, Lee S, Wang E, Lee AK, Talbot A, Ma C, Tsopoulidis N, Brumbaugh J, Zhao Y, Roberts KG, Hogg SJ, Nomikou S, Ghebrechristos YE, Thandapani P, Mullighan CG, Hochedlinger K, Chen W, Abdel-Wahab O, Eyquem J, and Aifantis I
- Subjects
- Antigens, CD19 genetics, Antigens, CD19 metabolism, Cleavage And Polyadenylation Specificity Factor metabolism, Humans, Immunotherapy, Adoptive adverse effects, Membrane Glycoproteins metabolism, Polyadenylation, RNA, Messenger genetics, RNA, Messenger metabolism, Trans-Activators metabolism, Burkitt Lymphoma, Lymphoma, B-Cell, Precursor Cell Lymphoblastic Leukemia-Lymphoma drug therapy, Receptors, Chimeric Antigen metabolism
- Abstract
B cell progenitor acute lymphoblastic leukemia (B-ALL) treatment has been revolutionized by T cell-based immunotherapies-including chimeric antigen receptor T cell therapy (CAR-T) and the bispecific T cell engager therapeutic, blinatumomab-targeting surface glycoprotein CD19. Unfortunately, many patients with B-ALL will fail immunotherapy due to 'antigen escape'-the loss or absence of leukemic CD19 targeted by anti-leukemic T cells. In the present study, we utilized a genome-wide CRISPR-Cas9 screening approach to identify modulators of CD19 abundance on human B-ALL blasts. These studies identified a critical role for the transcriptional activator ZNF143 in CD19 promoter activation. Conversely, the RNA-binding protein, NUDT21, limited expression of CD19 by regulating CD19 messenger RNA polyadenylation and stability. NUDT21 deletion in B-ALL cells increased the expression of CD19 and the sensitivity to CD19-specific CAR-T and blinatumomab. In human B-ALL patients treated with CAR-T and blinatumomab, upregulation of NUDT21 mRNA coincided with CD19 loss at disease relapse. Together, these studies identify new CD19 modulators in human B-ALL., (© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.)
- Published
- 2022
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6. Dissecting dual roles of MyoD during lineage conversion to mature myocytes and myogenic stem cells.
- Author
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Yagi M, Ji F, Charlton J, Cristea S, Messemer K, Horwitz N, Di Stefano B, Tsopoulidis N, Hoetker MS, Huebner AJ, Bar-Nur O, Almada AE, Yamamoto M, Patelunas A, Goldhamer DJ, Wagers AJ, Michor F, Meissner A, Sadreyev RI, and Hochedlinger K
- Subjects
- Animals, Cell Differentiation genetics, Mice, Muscle Fibers, Skeletal, Muscle, Skeletal, Myoblasts metabolism, Stem Cells metabolism, Muscle Development genetics, MyoD Protein genetics, MyoD Protein metabolism
- Abstract
The generation of myotubes from fibroblasts upon forced MyoD expression is a classic example of transcription factor-induced reprogramming. We recently discovered that additional modulation of signaling pathways with small molecules facilitates reprogramming to more primitive induced myogenic progenitor cells (iMPCs). Here, we dissected the transcriptional and epigenetic dynamics of mouse fibroblasts undergoing reprogramming to either myotubes or iMPCs using a MyoD-inducible transgenic model. Induction of MyoD in fibroblasts combined with small molecules generated Pax7
+ iMPCs with high similarity to primary muscle stem cells. Analysis of intermediate stages of iMPC induction revealed that extinction of the fibroblast program preceded induction of the stem cell program. Moreover, key stem cell genes gained chromatin accessibility prior to their transcriptional activation, and these regions exhibited a marked loss of DNA methylation dependent on the Tet enzymes. In contrast, myotube generation was associated with few methylation changes, incomplete and unstable reprogramming, and an insensitivity to Tet depletion. Finally, we showed that MyoD's ability to bind to unique bHLH targets was crucial for generating iMPCs but dispensable for generating myotubes. Collectively, our analyses elucidate the role of MyoD in myogenic reprogramming and derive general principles by which transcription factors and signaling pathways cooperate to rewire cell identity., (© 2021 Yagi et al.; Published by Cold Spring Harbor Laboratory Press.)- Published
- 2021
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7. HIV-1 infection of CD4 T cells impairs antigen-specific B cell function.
- Author
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Kaw S, Ananth S, Tsopoulidis N, Morath K, Coban BM, Hohenberger R, Bulut OC, Klein F, Stolp B, and Fackler OT
- Subjects
- Animals, HEK293 Cells, HIV-1, Humans, Immune Evasion immunology, Lymphocyte Activation, Male, Mice, Mice, Inbred C57BL, B-Lymphocytes immunology, CD4-Positive T-Lymphocytes immunology, HIV Infections immunology
- Abstract
Failures to produce neutralizing antibodies upon HIV-1 infection result in part from B-cell dysfunction due to unspecific B-cell activation. How HIV-1 affects antigen-specific B-cell functions remains elusive. Using an adoptive transfer mouse model and ex vivo HIV infection of human tonsil tissue, we found that expression of the HIV-1 pathogenesis factor NEF in CD4 T cells undermines their helper function and impairs cognate B-cell functions including mounting of efficient specific IgG responses. NEF interfered with T cell help via a specific protein interaction motif that prevents polarized cytokine secretion at the T-cell-B-cell immune synapse. This interference reduced B-cell activation and proliferation and thus disrupted germinal center formation and affinity maturation. These results identify NEF as a key component for HIV-mediated dysfunction of antigen-specific B cells. Therapeutic targeting of the identified molecular surface in NEF will facilitate host control of HIV infection., (© 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
- Published
- 2020
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8. NHC-gold compounds mediate immune suppression through induction of AHR-TGFβ1 signalling in vitro and in scurfy mice.
- Author
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Cheng X, Haeberle S, Shytaj IL, Gama-Brambila RA, Theobald J, Ghafoory S, Wölker J, Basu U, Schmidt C, Timm A, Taškova K, Bauer AS, Hoheisel J, Tsopoulidis N, Fackler OT, Savarino A, Andrade-Navarro MA, Ott I, Lusic M, Hadaschik EN, and Wölfl S
- Subjects
- Animals, Basic Helix-Loop-Helix Transcription Factors metabolism, Cell Survival drug effects, Hep G2 Cells, Humans, Male, Mice, Receptors, Aryl Hydrocarbon metabolism, Transforming Growth Factor beta1 metabolism, Basic Helix-Loop-Helix Transcription Factors genetics, Immunosuppression Therapy methods, Organogold Compounds immunology, Receptors, Aryl Hydrocarbon genetics, Signal Transduction drug effects, Transforming Growth Factor beta1 genetics
- Abstract
Gold compounds have a long history of use as immunosuppressants, but their precise mechanism of action is not completely understood. Using our recently developed liver-on-a-chip platform we now show that gold compounds containing planar N -heterocyclic carbene (NHC) ligands are potent ligands for the aryl hydrocarbon receptor (AHR). Further studies showed that the lead compound (MC3) activates TGFβ1 signaling and suppresses CD4
+ T-cell activation in vitro, in human and mouse T cells. Conversely, genetic knockdown or chemical inhibition of AHR activity or of TGFβ1-SMAD-mediated signaling offsets the MC3-mediated immunosuppression. In scurfy mice, a mouse model of human immunodysregulation polyendocrinopathy enteropathy X-linked syndrome, MC3 treatment reduced autoimmune phenotypes and extended lifespan from 24 to 58 days. Our findings suggest that the immunosuppressive activity of gold compounds can be improved by introducing planar NHC ligands to activate the AHR-associated immunosuppressive pathway, thus expanding their potential clinical application for autoimmune diseases., Competing Interests: Competing interestsX.C., I.O., and S.W. have filed a result-related patent application. All other authors declare no competing interests., (© The Author(s) 2020.)- Published
- 2020
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9. GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.
- Author
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Wang Y, Sherrard A, Zhao B, Melak M, Trautwein J, Kleinschnitz EM, Tsopoulidis N, Fackler OT, Schwan C, and Grosse R
- Subjects
- Actin Cytoskeleton metabolism, Animals, Calcium Signaling, Chromatin genetics, Endoplasmic Reticulum metabolism, HEK293 Cells, Humans, Ligands, Mice, Mitochondria metabolism, NIH 3T3 Cells, Polymerization, Receptors, G-Protein-Coupled genetics, Actins metabolism, Calcium metabolism, Cell Nucleus metabolism, Chromatin metabolism, Receptors, G-Protein-Coupled metabolism
- Abstract
Although the properties of the actin cytoskeleton in the cytoplasm are well characterized, the regulation and function of nuclear actin filaments are only recently emerging. We previously demonstrated serum-induced, transient assembly of filamentous actin within somatic cell nuclei. However, the extracellular cues, cell surface receptors as well as underlying signaling mechanisms have been unclear. Here we demonstrate that physiological ligands for G protein-coupled receptors (GPCRs) promote nuclear F-actin assembly via heterotrimeric Gα
q proteins. Signal-induced nuclear actin responses require calcium release from the endoplasmic reticulum (ER) targeting the ER-associated formin INF2 at the inner nuclear membrane (INM). Notably, calcium signaling promotes the polymerization of linear actin filaments emanating from the INM towards the nuclear interior. We show that GPCR and calcium elevations trigger nuclear actin-dependent alterations in chromatin organization, uncovering a general cellular mechanism by which physiological ligands and calcium promote nuclear F-actin assembly for rapid responses towards chromatin dynamics.- Published
- 2019
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10. HIV-1 Nef Disrupts CD4 + T Lymphocyte Polarity, Extravasation, and Homing to Lymph Nodes via Its Nef-Associated Kinase Complex Interface.
- Author
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Lamas-Murua M, Stolp B, Kaw S, Thoma J, Tsopoulidis N, Trautz B, Ambiel I, Reif T, Arora S, Imle A, Tibroni N, Wu J, Cui G, Stein JV, Tanaka M, Lyck R, and Fackler OT
- Subjects
- Animals, Binding Sites, CD4-Positive T-Lymphocytes immunology, Humans, Lymph Nodes immunology, Lymphocyte Activation immunology, Mice, Mice, Inbred C57BL, CD4-Positive T-Lymphocytes virology, Cell Polarity immunology, Chemotaxis, Leukocyte immunology, Transendothelial and Transepithelial Migration immunology, nef Gene Products, Human Immunodeficiency Virus metabolism
- Abstract
HIV-1 Nef is a multifunctional protein that optimizes virus spread and promotes immune evasion of infected cells to accelerate disease progression in AIDS patients. As one of its activities, Nef reduces the motility of infected CD4
+ T lymphocytes in confined space. In vivo, Nef restricts T lymphocyte homing to lymph nodes as it reduces the ability for extravasation at the diapedesis step. Effects of Nef on T lymphocyte motility are typically mediated by its ability to reduce actin remodeling. However, interference with diapedesis does not depend on residues in Nef required for inhibition of host cell actin dynamics. In search for an alternative mechanism by which Nef could alter T lymphocyte extravasation, we noted that the viral protein interferes with the polarization of primary human CD4+ T lymphocytes upon infection with HIV-1. Expression of Nef alone is sufficient to disrupt T cell polarization, and this effect is conserved among lentiviral Nef proteins. Nef acts by arresting the oscillation of CD4+ T cells between polarized and nonpolarized morphologies. Mapping studies identified the binding site for the Nef-associated kinase complex (NAKC) as critical determinant of this Nef activity and a NAKC-binding-deficient Nef variant fails to impair CD4+ T lymphocyte extravasation and homing to lymph nodes. These results thus imply the disruption of T lymphocyte polarity via its NAKC binding site as a novel mechanism by which lentiviral Nef proteins alter T lymphocyte migration in vivo., (Copyright © 2018 by The American Association of Immunologists, Inc.)- Published
- 2018
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11. Sensing of HIV-1 Infection in Tzm-bl Cells with Reconstituted Expression of STING.
- Author
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Trotard M, Tsopoulidis N, Tibroni N, Willemsen J, Binder M, Ruggieri A, and Fackler OT
- Subjects
- Cell Line, Cytokines biosynthesis, Cytokines genetics, Gene Expression, Humans, Immune Evasion, Interferon Regulatory Factor-3 metabolism, Membrane Proteins genetics, Protein Transport, vpr Gene Products, Human Immunodeficiency Virus genetics, vpr Gene Products, Human Immunodeficiency Virus metabolism, HIV-1 growth & development, HIV-1 immunology, Host-Pathogen Interactions, Membrane Proteins biosynthesis
- Abstract
Unlabelled: Production of proinflammatory cytokines indicative of potent recognition by the host innate immune system has long been recognized as a hallmark of the acute phase of HIV-1 infection. The first components of the machinery by which primary HIV target cells sense infection have recently been described; however, the mechanistic dissection of innate immune recognition and viral evasion would be facilitated by an easily accessible cell line model. Here we describe that reconstituted expression of the innate signaling adaptor STING enhanced the ability of the well-established HIV reporter cell line Tzm-bl to sense HIV infection and to convert this information into nuclear translocation of IRF3 as well as expression of cytokine mRNA. STING-dependent immune sensing of HIV-1 required virus entry and reverse transcription but not genome integration. Particularly efficient recognition was observed for an HIV-1 variant lacking expression of the accessory protein Vpr, suggesting a role of the viral protein in circumventing STING-mediated immune signaling. Vpr as well as STING significantly impacted the magnitude and breadth of the cytokine mRNA expression profile induced upon HIV-1 infection. However, cytoplasmic DNA sensing did not result in detectable cytokine secretion in this cell system, and innate immune recognition did not affect infection rates. Despite these deficits in eliciting antiviral effector functions, these results establish Tzm-bl STING and Tzm-bl STING IRF3.GFP cells as useful tools for studies aimed at dissecting mechanisms and regulation of early innate immune recognition of HIV infection., Importance: Cell-autonomous immune recognition of HIV infection was recently established as an important aspect by which the host immune system attempts to fend off HIV-1 infection. Mechanistic studies on host cell recognition and viral evasion are hampered by the resistance of many primary HIV target cells to detailed experimental manipulation. We describe here that expression of the signaling adaptor STING renders the well-established HIV reporter cell line Tzm-bl competent for innate recognition of HIV infection. Key characteristics reflected in this cell model include nuclear translocation of IRF3, expression of a broad range of cytokine mRNAs, and an antagonistic activity of the HIV-1 protein Vpr. These results establish Tzm-bl STING and Tzm-bl STING IRF3.GFP cells as a useful tool for studies of innate recognition of HIV infection., (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
- Published
- 2015
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12. Association with PAK2 Enables Functional Interactions of Lentiviral Nef Proteins with the Exocyst Complex.
- Author
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Imle A, Abraham L, Tsopoulidis N, Hoflack B, Saksela K, and Fackler OT
- Subjects
- Actins metabolism, Humans, Immune Evasion, Protein Binding, HIV-1 physiology, Vesicular Transport Proteins metabolism, nef Gene Products, Human Immunodeficiency Virus metabolism, p21-Activated Kinases metabolism
- Abstract
Unlabelled: Human immunodeficiency virus type 1 (HIV-1) Nef enhances virus replication and contributes to immune evasion in vivo, but the underlying molecular mechanisms remain incompletely defined. Nef interferes with host cell actin dynamics to restrict T lymphocyte responses to chemokine stimulation and T cell receptor engagement. This relies on the assembly of a labile multiprotein complex including the host kinase PAK2 that Nef usurps to phosphorylate and inactivate the actin-severing factor cofilin. Components of the exocyst complex (EXOC), an octameric protein complex involved in vesicular transport and actin remodeling, were recently reported to interact with Nef via the same molecular surface that mediates PAK2 association. Exploring the functional relevance of EXOC in Nef-PAK2 complex assembly/function, we found Nef-EXOC interactions to be specifically mediated by the PAK2 interface of Nef, to occur in infected human T lymphocytes, and to be conserved among lentiviral Nef proteins. In turn, EXOC was dispensable for direct downstream effector functions of Nef-associated PAK2. Surprisingly, PAK2 was essential for Nef-EXOC association, which required a functional Rac1/Cdc42 binding site but not the catalytic activity of PAK2. EXOC was dispensable for Nef functions in vesicular transport but critical for inhibition of actin remodeling and proximal signaling upon T cell receptor engagement. Thus, Nef exploits PAK2 in a stepwise mechanism in which its kinase activity cooperates with an adaptor function for EXOC to inhibit host cell actin dynamics., Importance: Human immunodeficiency virus type 1 (HIV-1) Nef contributes to AIDS pathogenesis, but the underlying molecular mechanisms remain incompletely understood. An important aspect of Nef function is to facilitate virus replication by disrupting T lymphocyte actin dynamics in response to stimulation via its association with the host cell kinase PAK2. We report here that the molecular surface of Nef for PAK2 association also mediates interaction of Nef with EXOC and establish that PAK2 provides an essential adaptor function for the subsequent formation of Nef-EXOC complexes. PAK2 and EXOC specifically cooperate in the inhibition of actin dynamics and proximal signaling induced by T cell receptor engagement by Nef. These results establish EXOC as a functionally relevant Nef interaction partner, emphasize the suitability of the PAK2 interaction surface for future therapeutic interference with Nef function, and show that such strategies need to target activity-independent PAK2 functions., (Copyright © 2015 Imle et al.)
- Published
- 2015
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