Back to Search
Start Over
GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.
- Source :
-
Nature communications [Nat Commun] 2019 Nov 21; Vol. 10 (1), pp. 5271. Date of Electronic Publication: 2019 Nov 21. - Publication Year :
- 2019
-
Abstract
- Although the properties of the actin cytoskeleton in the cytoplasm are well characterized, the regulation and function of nuclear actin filaments are only recently emerging. We previously demonstrated serum-induced, transient assembly of filamentous actin within somatic cell nuclei. However, the extracellular cues, cell surface receptors as well as underlying signaling mechanisms have been unclear. Here we demonstrate that physiological ligands for G protein-coupled receptors (GPCRs) promote nuclear F-actin assembly via heterotrimeric Gα <subscript>q</subscript> proteins. Signal-induced nuclear actin responses require calcium release from the endoplasmic reticulum (ER) targeting the ER-associated formin INF2 at the inner nuclear membrane (INM). Notably, calcium signaling promotes the polymerization of linear actin filaments emanating from the INM towards the nuclear interior. We show that GPCR and calcium elevations trigger nuclear actin-dependent alterations in chromatin organization, uncovering a general cellular mechanism by which physiological ligands and calcium promote nuclear F-actin assembly for rapid responses towards chromatin dynamics.
- Subjects :
- Actin Cytoskeleton metabolism
Animals
Calcium Signaling
Chromatin genetics
Endoplasmic Reticulum metabolism
HEK293 Cells
Humans
Ligands
Mice
Mitochondria metabolism
NIH 3T3 Cells
Polymerization
Receptors, G-Protein-Coupled genetics
Actins metabolism
Calcium metabolism
Cell Nucleus metabolism
Chromatin metabolism
Receptors, G-Protein-Coupled metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 31754104
- Full Text :
- https://doi.org/10.1038/s41467-019-13322-y