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2 results on '"Trevor Zahl"'

1. Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic

Author

Anna Nemudraia, Artem Nemudryi, Murat Buyukyoruk, Andrew M. Scherffius, Trevor Zahl, Tanner Wiegand, Shishir Pandey, Joseph E. Nichols, Laina N. Hall, Aidan McVey, Helen H. Lee, Royce A. Wilkinson, Laura R. Snyder, Joshua D. Jones, Kristin S. Koutmou, Andrew Santiago-Frangos, and Blake Wiedenheft

Subjects
Science
Abstract

Many viral diagnostic approaches require nucleic acid extraction and amplification prior to detection. Here, the authors develop a method based on type III CRISPR systems which allows sequence specific capture, concentration, and detection of viral RNA directly from patient samples.

Published
2022
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2. CRISPR-based engineering of RNA viruses

Author

Artem Nemudryi, Anna Nemudraia, Joseph E Nichols, Andrew M Scherffius, Trevor Zahl, and Blake Wiedenheft

Subjects
Article
Abstract

CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a new recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.One-Sentence SummaryProgrammable CRISPR RNA-guided ribonucleases enable recombinant RNA technology.

Published
2023

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