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Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic

Authors :
Anna Nemudraia
Artem Nemudryi
Murat Buyukyoruk
Andrew M. Scherffius
Trevor Zahl
Tanner Wiegand
Shishir Pandey
Joseph E. Nichols
Laina N. Hall
Aidan McVey
Helen H. Lee
Royce A. Wilkinson
Laura R. Snyder
Joshua D. Jones
Kristin S. Koutmou
Andrew Santiago-Frangos
Blake Wiedenheft
Source :
Nature Communications, Vol 13, Iss 1, Pp 1-12 (2022)
Publication Year :
2022
Publisher :
Nature Portfolio, 2022.

Abstract

Many viral diagnostic approaches require nucleic acid extraction and amplification prior to detection. Here, the authors develop a method based on type III CRISPR systems which allows sequence specific capture, concentration, and detection of viral RNA directly from patient samples.

Subjects

Subjects :
Science

Details

Language :
English
ISSN :
20411723
Volume :
13
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
edsdoj.88a12a41b642dfb6cdf759b457932d
Document Type :
article
Full Text :
https://doi.org/10.1038/s41467-022-35445-5