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1. Cellular and subcellular localizations of annexins I, IV, and VI in lung epithelia

Author

Mayran, N., Traverso, V., Maroux, S., and Massey-Harroche, D.

Subjects
Proteins -- Physiological aspects, Trachea -- Physiological aspects, Biological sciences
Abstract

Annexins are cytoplasmic proteins thought to play an important role in the regulation of secretion and in the anti-inflammatory action of glucocorticoids. It has been suggested that the biological functions of annexins are related to their cellular and subcellular localizations. Herein, the functions of annexins I, IV and VI in the lung epithelia were investigated in relation to their respective localizations. Annexin I was detected in ciliated cells, annexin VI in all lung epithelial cells and annexin VI in the alveolar endothelial cells.

Published
1996

5. Les séquences de traduction spontanée comme mécanisme de réparation dans des interactions professionnelles

Author

Merlino S., Traverso V., Merlino, S., and Traverso, V.

Abstract

This paper deals with a phenomenon observed in multilingual meetings during which one participant, who is translating for the others, confronts a lexical problem and tries to solve it by asking his interlocutors for a translation. This "word search in another language" phenomenon is a type of repair and implies the participation of all the interlocutors, who stop the main activity in order to focus on the word search. The analysis shows the three phases sequential development of the phenomenon: opening, collaboration and closing of the search. The collaboration of the interlocutors, which, in our examples, is initiated by the current speaker's explicit request for translation and by code-switching, modifies the participation framework and frequently leads to the creation of schisms. By initiating and solving the word search through the use of other languages than the interaction current one, participants show their orientation towards the multilingual character of the context and of the available resources, as well as an implicit categorization of each other linguistic competencies and of the activity of translationINGLESE Cet article porte sur l’analyse d’un phénomène interactionnel observé dans des réunions de travail plurilingues au cours desquelles un participant en train de traduire manifeste un problème lexical et tente de le résoudre en demandant une traduction à ses interlocuteurs. Ce phénomène de recherche de mot dans une autre langue est une forme de réparation, et se caractérise comme un travail collectif auquel tous les participants contribuent, en suspendant momentanément l’activité en cours et en se focalisant sur l’activité de recherche elle-même. L’analyse met en évidence le développement séquentiel du phénomène, notamment, la succession de trois phases dans la recherche: l’ouverture, la collaboration, l’issue. La collaboration des interlocuteurs, occasionnée, dans nos extraits, par une demande explicite de traduction de la part du locuteur en difficulté et par l’alternance codique, montre, entre autres, une évolution du cadre participatif en jeu, et la possibilité de scissions de ce cadre. Le fait que les participants initient et résolvent la recherche de mot en passant par d’autres langues que celle de l’interaction, manifeste leur orientation vers le caractère plurilingue du contexte et des ressources à leur disposition, ainsi qu’une catégorisation implicite de leurs compétences linguistiques réciproques et de l’activité de traduction FRANCESE

Published
2009

6. «La relation de limitation et d’exception dans le français d’aujourd’hui : excepté, sauf et hormis comme pivots d’une relation algébrique »

Author

DURAND J, HABERT B., LAKS B, HABERT B, MUNI TOKE V., GREKAS N, COSTE A, FRADIN B., G. DAL, BLUMENTHAL P, BRONCKART J. P, FUCHS C, KAYNE R, KLEIBER G, MARCHELLO NIZIA C, MORIN YC, NØLKE H, COMBETTES B., DE MULDER W, LODGE A, PREVOST S, BADIOU MONFERRAN C, BUCHI E., STADTLER T, COLTIER D., FERON C, DUFRESNE M, F. DUPUIS, TREMBLAY M, DUPUY PARANT E, FAGARD B., DEGAND L, GLIKMAN J, KAWAGUCHI Y, LANDVOGT A., GOLDSCHMITT S, MAZZIOTTA N, MOKNI M, MORTELMANS J, NOSKE R, POUTEAUX M. A, ROSSI GENSANE R, ELALOUF M. L, GARCIA DEBANC C, MANGENOT F, PLANE S, VERONIQUE GD, BARZABAN M, BOUCHARD R., PARPETTE C, BRISSAUD C., COGIS D, CANELAS TREVISI S, CARCASSONNE M, FROMENT M., SALAGNAC N, CHARTRAND S, CRINON J, MARIN B., CAUTELA A, DETEY S., LE GAC D, DOQUET LACOSTE C, LECLAIRE HALTE A, MASSERON C., LUSTE CHAA O, MULLER C, PAOLACCI V., QUANQUIN V, RONDELLI F, THOMAS A, BRES J, GUELICH E, MONDADA L, AUGER N, RACCHIOLLA B, MOISE C., SCHULTZ ROMAIN C, BARBU V, BASSLER V, BENAZZO S, BERT M, BRUXELLES S, ETIENNE C, TESTON S., TRAVERSO V, DE STEFANI E, FERRE G, HO DAC L. M., PERY WOODLEY M. P, HORLACHER A. S, JULLIEN S, OLOFF F, PEKAREK S, PEPIN N, SCHMALE G, STEINBACH KOHLER F, FASEL LAUZON V., BERGER E, AUROUX S, COLOMBAT B, NEVEU F, PRANDI M, PUECH C, BOUARD B, CUREA A, LEON J, POIBEAU T, RABY V., FOURNIER J. M, REY C, TCHOUGOUNNIKOV S, TESTENOIRE P. Y, WAUTHION M, TOUTAIN A. G, CORBIN P, MARTIN R, PIERREL J. M, POLGUERE A, ANDRONACHE M, DEBRENNE M, REY C., MOREL M. A, GASIGLIA N, LIGAS P, LILLO J, MICHOT N, MONTEMONT V, STEUCKARDT A, TORTERAT F, AGRESTI G, COLAS BLAISE M, DELORMAS P, DETRIE C, GERARD C, HERSCHBERG A, LAIPPALA V, LANE P, LECOMPTE J, MAGRI MOURGUES V, MONTE M, REVAZ F, SMADJA S, WULF J, YOCARIS I., ZEMMOUR D, ADAM J. M, BONHOMME M, JAUBERT A, MAINGUENEAU D, MOLINIE G, DAL G, PLENAT M, RAINER F, BONAMI O, BOYE G, GIRAUDO H., VOGA M, FRADIN B, GRABAR N, NAMER F, LIGNON S, PLANCQ C, ZWEIGENBAUM P., YVON F, FEVRE PERNET C, NAMER F., VILLOING F, ROCHE M, ANGOUJARD J. P, BOURHIS V, CHASLE N, RANSON D, SIMON AC, AVANZI M., GODMAN J. P, DEMIRDACHE H, DUVIGNAU K, WAUQUIER S, BEZINSKA Y, CHEVROT J. P., NOVAKOVA I, GAUME B, TRAN TM, MANCHON M, MARTINOT C., PANISSAL N, EISENKOLB B, FOUCAMBERT D, LUNGU O., MACOIR J., FOSSARD M, MONVILLE BURSTON M, MORGENSTERN A, LEROY M., MATHIOT E, PARISSE C., MOLLIER R, SERGEEVA E., CHEVROT J. P, TRAN TM, TRANCART M., SERVENT D, TSEDRYK K., PUNKO I, FRANÇOIS J, GEERAERTS D, PEETERS B, STEIN A, ABDOULHAMID A, ADLER S., ASNES M, AURNAGUE M, BAIDER F., JACQUEY E, BERTELS A, BRAS M, PREVOT L., VERGEZ COURET M, CORBLIN F, COSTACHESCU A, EMIRKANIAN L, GREA P, HAAS P, HUYGHE R., MARIN R, KANEKO M, KOSELAK A, LE BELLEC C, LONGHI J, MAZIERE F., DELESALLE S, PLANCHON P, SCHNEDECKER C, VASSILIADOU H., LAMMERT M, VAXELAIRE JL, VEECOCK C, AKISSI BOUTIN B, BERGOUNIOUX G, FAGYAL Z., STEWART C, GUERIN E, LABEAU E, LAUR E, TATOSSIAN A, TRAN TBM, ABEILLE A, GODARD D., SABIO F, ACHARD M, BEAUSEROY D, BONAMI O., GODARD D, BUCHARD A., CARLIER A, CORMINBOEUF G, DAGNAC A, DARGNAT M, FABRE C, REBEYROLLE J., HO DAC L. M, GAATONE D, GUSHCHINA O, HAVU E., PIERRARD M, KAHANE S, LE PESANT D, MOURET F, NAKAMURA T, HADERMANN P, VAN RAEMDONCK D., WIELEMANS V, SAEZ F, TSENG J, ZRIBI HERTZ A, LEGENDRE G., CULBERTSON J, MULLER C., RUOZZI, Paola, DURAND J, HABERT B & LAKS B, HABERT B, MUNI TOKE V & GREKAS N, COSTE A, FRADIN B & G DAL, BLUMENTHAL P, BRONCKART J-P, FUCHS C, KAYNE R, KLEIBER G, MARCHELLO-NIZIA C, MORIN YC, NØLKE H, COMBETTES B & MARCHELLO-NIZIA C, DE MULDER W, LODGE A, PREVOST S, BADIOU-MONFERRAN C, BUCHI E & STADTLER T, COLTIER D & FERON C, DUFRESNE M, F DUPUIS & TREMBLAY M, DUPUY-PARANT E, FAGARD B & DEGAND L, GLIKMAN J, KAWAGUCHI Y, LANDVOGT A & GOLDSCHMITT S, MAZZIOTTA N, MOKNI M, MORTELMANS J, NOSKE R, POUTEAUX M-A, ROSSI-GENSANE R, ELALOUF M-L, GARCIA-DEBANC C, MANGENOT F, PLANE S, VERONIQUE GD, BARZABAN M, BOUCHARD R & PARPETTE C, BRISSAUD C & COGIS D, CANELAS-TREVISI S, CARCASSONNE M, FROMENT M & SALAGNAC N, CHARTRAND S, CRINON J, MARIN B & CAUTELA A, DETEY S & LE GAC D, DOQUET-LACOSTE C, LECLAIRE-HALTE A, MASSERON C & LUSTE-CHAA O, MULLER C, PAOLACCI V & GARCIA-DEBANC C, QUANQUIN V, RONDELLI F, THOMAS A, BRES J, GUELICH E, MONDADA L, AUGER N, RACCHIOLLA B, MOISE C & SCHULTZ-ROMAIN C, BARBU V, BASSLER V, BENAZZO S, BERT M, BRUXELLES S, ETIENNE C, MONDADA L, TESTON S & TRAVERSO V, DE STEFANI E, FERRE G, HO-DAC L-M & PERY-WOODLEY M-P, HORLACHER A-S, JULLIEN S, OLOFF F, PEKAREK S, PEPIN N, SCHMALE G, STEINBACH KOHLER F, FASEL LAUZON V & BERGER E, AUROUX S, COLOMBAT B, NEVEU F, PRANDI M, PUECH C, BOUARD B, CUREA A, LEON J, POIBEAU T, RABY V & FOURNIER J-M, REY C, TCHOUGOUNNIKOV S, TESTENOIRE P-Y, WAUTHION M, TOUTAIN A-G, CORBIN P, MARTIN R, PIERREL J-M, POLGUERE A, ANDRONACHE M, DEBRENNE M, REY C & MOREL M-A, GASIGLIA N, LIGAS P, LILLO J, MICHOT N, MONTEMONT V, STEUCKARDT A, TORTERAT F, AGRESTI G, COLAS-BLAISE M, DELORMAS P, DETRIE C, GERARD C, HERSCHBERG A, LAIPPALA V, LANE P, LECOMPTE J, MAGRI-MOURGUES V, MONTE M, REVAZ F, SMADJA S, WULF J, YOCARIS I & ZEMMOUR D, ADAM J-M, BONHOMME M, JAUBERT A, MAINGUENEAU D, MOLINIE G, DAL G, PLENAT M, RAINER F, BONAMI O, BOYE G, GIRAUDO H & VOGA M, DAL G, FRADIN B, GRABAR N, NAMER F, LIGNON S, PLANCQ C, ZWEIGENBAUM P & YVON F, FEVRE-PERNET C, NAMER F & VILLOING F, ROCHE M, ANGOUJARD J-P, BOURHIS V, CHASLE N, RANSON D, SIMON AC, AVANZI M & GODMAN J-P, DEMIRDACHE H, DUVIGNAU K, WAUQUIER S, BEZINSKA Y, CHEVROT J-P & NOVAKOVA I, DUVIGNAU K, GAUME B, TRAN TM, MANCHON M, MARTINOT C & PANISSAL N, EISENKOLB B, FOUCAMBERT D, LUNGU O & DEMIRDACHE H, MACOIR J & FOSSARD M, MONVILLE-BURSTON M, MORGENSTERN A, LEROY M & MATHIOT E, PARISSE C & MOLLIER R, SERGEEVA E & CHEVROT J-P, TRAN TM, TRANCART M & SERVENT D, TSEDRYK K & PUNKO I, FRANÇOIS J, GEERAERTS D, PEETERS B, STEIN A, ABDOULHAMID A, ADLER S & ASNES M, AURNAGUE M, BAIDER F & JACQUEY E, BERTELS A, BRAS M, PREVOT L & VERGEZ-COURET M, CORBLIN F, COSTACHESCU A, EMIRKANIAN L, FOSSARD M, GREA P, HAAS P, HUYGHE R & MARIN R, KANEKO M, KOSELAK A, LE BELLEC C, LONGHI J, MAZIERE F & DELESALLE S, PLANCHON P, SCHNEDECKER C, VASSILIADOU H & LAMMERT M, VAXELAIRE JL, VEECOCK C, AKISSI-BOUTIN B, BERGOUNIOUX G, FAGYAL Z & STEWART C, GUERIN E, LABEAU E, LAUR E, TATOSSIAN A, TRAN TBM, ABEILLE A, GODARD D & SABIO F, ACHARD M, BEAUSEROY D, BONAMI O & GODARD D, BUCHARD A & CARLIER A, CORMINBOEUF G, DAGNAC A, DARGNAT M, FABRE C, REBEYROLLE J & HO-DAC L-M, GAATONE D, GUSHCHINA O, HAVU E & PIERRARD M, KAHANE S, LE PESANT D, MOURET F, NAKAMURA T, PIERRARD M, HADERMANN P, VAN RAEMDONCK D & WIELEMANS V, RUOZZI P, SAEZ F, TSENG J, ZRIBI-HERTZ A, and LEGENDRE G & CULBERTSON J

Subjects
algèbre, préposition, exception, limitation, relation algébrique, Settore L-LIN/04 - Lingua E Traduzione - Lingua Francese, algébrique, valeur algébrique
Abstract

L’analyse des emplois prépositionnels et des emplois conjonctifs d’ “excepté”, de “sauf” et d’ “hormis” permet d’envisager les trois prépositions/conjonctions comme le pivot d’un binôme, comme la plaque tournante d’une structure bipolaire. Placées au milieu du binôme, ces prépositions sont forcées par leur sémantisme originaire dûment métaphorisé de jouer le rôle de marqueurs d’inconséquence systématique entre l’élément se trouvant à leur gauche et celui qui se trouve à leur droite. L’opposition qui surgit entre les deux éléments n’est donc pas une incompatibilité naturelle, intrinsèque, mais extrinsèque, induite. Dans la plupart des cas (emplois limitatifs), cette opposition prend la forme d’un rapport entre une « classe » et le « membre (soustrait) de la classe », ou bien entre un « tout » et une « partie » ; dans d’autres (emplois exceptifs), cette opposition se manifeste au contraire comme une attaque de front portée par un « tout » à un autre « tout ». De plus, l’inconséquence induite mise en place par la préposition/conjonction paraît, en principe, tout à fait insurmontable. Dans l’assertion « les écureuils vivent partout, sauf en Australie » (que l’on peut expliciter par « Les écureuils vivent partout, sauf [qu’ils ne vivent pas] en Australie »), la préposition semble en effet capable d’impliquer le prédicat principal avec signe inverti, et de bâtir sur une telle implication une sorte de sous énoncé qui, à la rigueur, est totalement inconséquent avec celui qui le précède (si « les écureuils ne vivent pas en Australie », le fait qu’ils « vivent partout » est faux). Néanmoins, l’analyse montre qu’alors que certaines de ces oppositions peuvent enfin être dépassées, d’autres ne le peuvent pas. C’est, respectivement, le cas des relations limitatives et des relations exceptives. La relation limitative, impliquant le rapport « tout » - « partie », permet de résoudre le conflit dans les termes d’une somme algébrique entre deux sous énoncés pourvus de différent poids informatif et de signe contraire. Les valeurs numériques des termes de la somme étant déséquilibrées, le résultat est toujours autre que zéro. La relation exceptive, au contraire, qui n’implique pas le rapport « tout » - « partie », n’est pas capable de résoudre le conflit entre deux sous énoncés pourvus du même poids informatif et en même temps de signe contraire : les valeurs numériques des termes de la somme étant symétriques et égales, le résultat sera toujours équivalent à zéro.

Published
2008

9. Aspects of activity coordination between two cooks in a professional setting

Author

GALATOLO, RENATA, Traverso V., Galatolo R., and Traverso V.

Subjects
ACTIONS' CONFIGURATION, COMMUNICATIVE MULTI-MODALITY, PROFESSIONAL INTERACTION, ACTIVITIES' COORDINATION, COOKING
Abstract

In this paper, we analyse data from a multi-view video recording (with two or three cameras) of two cooks preparing meals in the kitchen of an Italian restaurant. The research program focuses on the verbal and non verbal modalities that the two cooks use for coordinating the activity of preparing a single dish or a sequence of dishes. The level of their coordination changes along with the different phases of the activity. The minimum level of coordination consists in a mutual attention which permits them to accomplish independent activities in sharing the different working emplacements of the kitchen. The maximum level of coordination corresponds to the co-accomplishment of a phase in sequence of actions. And between the two, various configurations can occur. Besides these cases, there are occasions in which one of the participants begins an action which is then interrupted or suspended by the intervention of the other participant or by her becoming available for accomplishing a particular task at that moment. The intervention of the other participant changes the configuration of the whole initiated action . The analyses will focus on those particular cases, which permit us to investigate the transition between individual actions and coordinated activity.

Published
2005

10. Cooking together: activity coordination in professional cooking

Author

GALATOLO, RENATA, Traverso V., Galatolo R., and Traverso V.

Subjects
CO-ACTIVITY, SEQUENTIAL ORGANIZATION, PROFESSIONAL INTERACTION, COOKING
Abstract

The analysis focuses on verbal and non verbal modalities professional cooks use to accomplish a joined activity, which is the preparation of a meal. The data are taken from the video recording of two cooks preparing meals in the kitchen of an Italian restaurant. The activity has been recorded with two cameras from two different points of view in order to obtain a more complete vision of the entire activity. The total amount of data corresponds to the preparation of four meals. The cooks are preparing the meal following the orders which are communicated by the waitress. For communicating the orders, the waitress enters the kitchen saying the order aloud and hanging up the written version of the order on the wall. The written order hanging on the wall functions as reference for the cooks’ evaluation of the sequential arrangement of the actions they are accomplishing. The analysis shows how, in the context of a complex and shared activity, the accomplishment of an action can project the following relevant action organizing the activity of all participants. The performance of single actions, for example the action of putting a pot in a specific place or describing aloud what one’s is doing, can function as an organizational device of the whole activity of co-preparing meals.

Published
2005

11. Differential Expression of matrix Attachment Region-Binding Proteins in Human Prostatic cancer cell lines

Author

Barboro P., Repaci E., Traverso V., D'Arrigo C., and Balbi C.

Subjects
nuclear matrix, diagnostic and prognostic markers, prostate cancer
Abstract

Background: Abnormal nuclear organization and alterations in the amount and distribution of heterochromatin have always been recognized as hallmarks of human cancer. However, neither the exact cause of these modifications nor how the activity/silencing of thousands genes can be orchestrated are currently known. In eukaryotes, the genome is compartmentalized into chromatin domains by attachment to a supporting structuretermed the nuclear matrix (NM). The interactions of chromatin with the NM occur via AT-rich DNA sequences called matrix attachment regions (MARs). MARs exibit several functions including the organization of chromatin loops, the augmentation of transcription and the facilitation of replication; moreover, the attachment of the genome to the NM is a dynamic event that is cell type or cell cycle-dependent. Several MAR-binding proteins have been identified, some are drammatically dysregulated in tumor cells and their expression is often significantly correlated with more aggressive phenotype. Likewise, modifications of the interactions between NM preoteins and MARs might be related to the large-scale chromatin organization observed in carcinogenesis. research carried out in our and other laboratories seeking prostate cancer (PCa) markers with improved diagnostic and prognostic features has identified some NMproteins that are differentially expressed in PCa with respect to non-tumor tissue; a few of these proteins were significantly correlated with tumor aggressiveness and/or risk of biochemical progression. These findings prompted us to characterize the changes in the NM protein-MAR bond in PCa. Materials and methods: in this study, using a proteomic approach along with two-dimensional Southwestern analysis, we charaterized the NM protein-MAR bond in two prostatic cancer cell lines: the androgen-responsive LNCaP and the androgen-resistant PC3. For binding experiments, a highly repetitive bent DNA sequence of 370-bp (XmnI) containing a base unpairing region was used as a probe. The variation of the expression of some MAR proteins was determined by quantitative Western blot. Both the nucleoplasm distribution and the co-localization with DNA were evaluated by confocal laser scanning microscopy. Using transmission electron microscopy (TEM) the high-order structure of chromatin was examined in the two cell lines. Results: several NM proteins extracted from both cell lines strongly bind to the XmnI sequence. It is possible to separate these proteins into two groups: I. MAR-binding proteins present in a large quantity also in other cellular types (MARPa, MARPb, hnRNPs, Lamin A, Lamin B, Matrin3) that may correspond to the proteins in contact with the constitutive MARs (i.e. the sequences demarcating permanent domain boundaries in all cell types). II. MAR-binding proteins corresponding to cell-type specific factors (PARPs, SATB1) that interact with facultative MARs (i.e. the sequences cell type- and activity- related and dependent on cell differentiation). In PC3 with respect to LNCaP cells, several changes in the ability of proteins to bind XmnI sequence were observed: the signal intensity of Matrin3, SATB1 and all basic hnRNPs decreased, showing that these proteins bind the DNA more weakly or are down-regulated in more aggressive cells. In contrast, MARPa, MARPb, PARP and its fragments increased. Confocal microscopy demonstrated that also the co-localization of PARP with DNA was significantly lower. These changes were concomitant with a different compartmentalization of chromatin in the two cell lines, as shown by TEM. Conclusion: our study provides evidence that several MAR-binding proteins undergo extensive changes in PC3 with respect to LNCaP cells, showing that the interactions between NM and the base of the chromatin loop are involved in cell differentiation. These proteins may turn out to be an important tool in the understanding of PCa carcinogenesis and may be novel targets of anticancer drugs.

Published
2011

15. Agenda Nacional de Investigación en Salud Publica

Author

Traverso Vior, Natacha, Chuit , Roberto, and Mejía, Raúl

Subjects
Medicine (General), R5-920, Public aspects of medicine, RA1-1270
Abstract

El 2 de diciembre de 2019 se presentó la Agenda Nacional de Investigación en Salud Pública en el Ministerio de Salud de Argentina. El establecimiento de prioridades en una Agenda Nacional de Investigación en Salud Pública es un proceso sistemático, participativo y reflexivo, que permite guiar la investigación hacia problemas relevantes, prevalentes y emergentes de la población, orientar los recursos hacia los temas priorizados, fortalecer y/o crear grupos, centros y redes de investigación para la salud pública, mejorar las capacidades para el desarrollo de investigación de calidad e incrementar la evidencia local para la toma de decisiones a fin de mejorar la salud de la población y los sistemas sanitarios en el marco de los Objetivos de Desarrollo Sustentable 2030. Como metodología para la elaboración de la Agenda se eligió el Método Delphi modificado por la Organización Panamericana de la Salud (OPS). En la elaboración de esta herramienta participaron la Dirección de Investigación para la Salud (DIS) del Ministerio de Salud de la Nación, a través de la Red Ministerial de Investigación en Salud (REMINSA), y la OPS. A lo largo de todo el proceso se consideró imprescindible lograr una amplia participación de actores de los niveles provinciales y nacionales, y de los sectores público y privado. La presencia de una Agenda Nacional de Investigación en Salud Pública permitirá generar investigación científica nacional y regional, e implementar políticas y recomendaciones que impacten en la salud de las poblaciones

Published
2019

16. Lipocortin 1 (annexin 1): a candidate paracrine agent localized in pituitary folliculo-stellate cells

Author

Traverso, V, Christian, H, Morris, J, and Buckingham, J

Abstract

It is now well established that lipocortin 1 (LC1) plays an important role as a mediator of early delayed glucocorticoid feedback action in the hypothalamo-hypophysial system. In both the hypothalamus and anterior pituitary gland, LC1 mimics some of the actions of glucocorticoids; moreover, glucocorticoids stimulate the synthesis of LC1 and cause the translocation of intracellular LC1 to the outer cell surface. The mechanism by which LC1 acts in these tissues is only partially understood, but may involve paracrine and/or autocrine actions. To address these possibilities we have investigated the localization of LC1 in the rat pituitary gland, using double labeling immunohistochemistry to identify the pituitary cell types that express LC1. At the light microscopic level LC1 was not detected in the endocrine cells in cryosections of the pituitary, but it was found in abundance in the surrounding folliculo-stellate (FS) cells. In the anterior and interme diate pituitary lobes, there was a near total colocalization of LC1 and S100, a specific marker of FS cells. By contrast, in the posterior pituitary gland, LC1 immunoreactivity was not colocalized with S100 which labeled most pituicytes, or with OX-42 monoclonal antibody, a marker of the microglial cells. Immunogold electron microscopy confirmed that LC1 is present in the nongranulated FS cells. LC1 im munoreactivity was also present in a mouse pituitary FS-like cell line (TtT/GF), particularly in the periphery of the cytoplasm. The localization of LC1 in the FS cells of the anterior pituitary gland defines LC1 as a new marker of the FS cell population. These results support our hypothesis that LC1 acts as one of the paracrine agents liberated by FS cells that modulate the release of pituitary hormones.

Published
1999

17. Are photoreceptors in the attention spotlight? Efferent neuromodulators accelerate and/or retard the time course of photoreceptor responses evoked by light

Author

Bolbecker, A. R., primary, Lim, C. C. M., additional, Li, J., additional, Traverso, V., additional, Orchard, A., additional, Christadoss, C. S., additional, Brahmbhatt, J., additional, Beck, K. E., additional, Lewis, A. R., additional, Fleet, J., additional, Carlson, K. W., additional, Hoyt, C., additional, Collins, M. D., additional, Swan, A., additional, and Wasserman, G. S., additional

Published
2010
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19. Les enjeux de la pragmatique contemporaine

Author

Conférence conjointe à l'Université de Tunis (février 1999: Tunis, Tunisie), Danblon, Emmanuelle, Traverso, V., Conférence conjointe à l'Université de Tunis (février 1999: Tunis, Tunisie), Danblon, Emmanuelle, and Traverso, V.

Abstract

info:eu-repo/semantics/nonPublished

Published
1999

21. Des pratiques de soutien par les pairs dans un Groupe d’Entraide Mutuelle

Author

Le Callonnec Aziliz, Traverso Véronique, Jouin Emilie, and Chambon Nicolas

Subjects
Social Sciences
Abstract

En croisant l'analyse d'entretiens à celles de données vidéo enregistrées dans le Groupe d'Entraide Mutuelle (GEM) Envol et Cie à Villeurbanne, l'article identifie et décrit quelques configurations in situ qui permettent de problématiser la mise en place de relations entre pairs, autour des notions de « similarité », « symétrie », « parité ». Ces mises en place sont examinées à partir des positionnements vis-à-vis des pairs aussi bien que des non-pairs (cas d'une réunion avec une animatrice non paire).

Published
2022
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27. Organisation du cadre participatif, accord et répétition dans l'interaction

Author

Traverso Véronique

Subjects
Social Sciences
Abstract

L'étude relève de l'analyse des interactions. Elle porte sur un certain usage des répétitions indiquant l'accord comme procédé permettant l'organisation de différentes "parties" au sein du cadre de participation global dans des situations (des réunions). Les questions traitées sont les suivantes : - comment les participants qui utilisent des hétéro-répétitions pour exprimer leur accord avec un autre participant parviennent-ils à rendre leur contribution distincte de celle du partenaire dont ils répètent l'énoncé ? - comment font-ils en sorte que leur propre voix ne se perde pas dans celle de la partie qu'ils contribuent à créer en répétant les propos d'un autre ? L'étude est basée sur un corpus de réunions de travail enregistrées en audio et en vidéo. L'analyse dégage trois contextes : le premier concerne un sens minimal de "partie", il est très local, c'est celui qui se met en place dans le cadre de l'échange question / réponse ; le second concerne des cas où deux parties sont opposées par un désaccord ; le troisième des cas où deux co-narrateurs s'adressent à un groupe. Elle permet de dégager des formes différentes des répétitions dans ces trois contextes. Les répétitions sont ensuite examinées en fonction de leur rôle vis-à-vis de celui qui est répété et vis-à-vis du reste du cadre de participation. Elles sont analysées en relation avec la façon dont elle permettent de négocier d'une part le droit à la parole et d'autre part l'autorité épistémique.

Published
2012
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28. Une méthode instrumentée pour l’analyse multidimensionnelle des tonalités émotionnelles dans l’interaction

Author

Quignard Matthieu, Ursi Biagio, Rossi-Gensane Nathalie, André Virginie, Baldauf-Quilliatre Heike, Etienne Carole, Plantin Christian, and Traverso Véronique

Subjects
Social Sciences
Abstract

Nous présentons une méthode et un outil exploratoire pour une approche interactionnelle de l’émotion dans les interactions. Cette méthode repose sur le repérage d’indices d’émotionnalité à travers huit dimensions d’analyse : la multimodalité, l’interactivité, les bruits-sons, les marques d’accord et de désaccord, les marques de personnes, les unités macrosyntaxiques, les unités interactionnelles et les répétitions. Deux extraits de corpus provenant de deux équipes différentes sont analysés suivant cette approche. L’outil de visualisation permet d’identifier et de mesurer les phénomènes de concomitance et d’accumulation d’indices à un instant donné, et ainsi d’appréhender la complexité de la construction de l’émotion dans l’interaction.

Published
2016
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29. Du coup dans l'interaction orale en français : description de ses usages situés à partir d'une base de données multimédia, et considérations didactiques

Author

Bruxelles, Sylvie, Jouin-Chardon, Emilie, Traverso, Véronique, Guinamard, Isabelle, Interactions, Corpus, Apprentissages, Représentations (ICAR), École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS), Guinamard I., Jouin-Chardon E., Traverso V., Thai T. D. (éds), Les auteurs remercient le LABEX ASLAN (ANR-10-LABX-0081) de l'Université de Lyon pour son soutien financier dans le cadre du programme 'Investissements d'Avenir' (ANR-11-IDEX-0007) de l'Etat Français géré par l'Agence Nationale de la Recherche (ANR)., ICAR, Référent HAL, Guinamard I., Jouin-Chardon E., Traverso V., Thai T. D. (éds), and École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS)

Subjects
apprentissage du français, outils pour l'enseignement, situations sociales, interaction, action, [SHS.LANGUE]Humanities and Social Sciences/Linguistics, [SHS.LANGUE] Humanities and Social Sciences/Linguistics, ComputingMilieux_MISCELLANEOUS, Corpus de langue parlée
Abstract

International audience

Published
2015

30. Accès aux émotions censurées lors d'interactions agents-clients

Author

Cahour, Béatrice, Laboratoire Traitement et Communication de l'Information (LTCI), Télécom ParisTech-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), Plantin Ch., Doury M., Traverso V., Cahour, Béatrice, and Plantin Ch., Doury M., Traverso V.

Subjects
[SHS.PSY] Humanities and Social Sciences/Psychology, censure, Interaction, émotions, relation de service, confiance, auto-confrontation, agent-client, [SHS.PSY]Humanities and Social Sciences/Psychology, masquage
Abstract

Lors des interactions de service, des ressentis circulent et une relation de confiance peut éventuellement se créer. On analyse ici quels sont les émotions vécues par deux clients lors d'une première rencontre avec un agent d'assurance, grâce à une technique de verbalisation consécutive avec support vidéo (autoconfrontation). On constate que des émotions d'énervement, impatience, honte, vexation, méfiance sont camouflées pendant l'interaction. On obtient donc ce qui est censuré par le client de son ressenti pendant l'interaction, et l'origine de la censure est interprétée notamment en termes de ménagement des faces. Des conclusions méthodologiques sont proposées, qui différencient trois niveaux d'accès aux émotions : une analyse 'externe' qui rend compte uniquement des émotions externalisés et non de celles qui sont contrôlées (réprimées, masquées, neutralisées) ; une analyse 'du point de vue du sujet', a posteriori, avec un accès à la censure qui a eu lieu pendant cette interaction, si le sujet est en confiance avec le chercheur ; une analyse 'dans un cadre clinique', où une censure psychique ou inconsciente pourrait éventuellement être levée.

Published
1997

31. Co-élaboration de solutions et rôle du graphico-gestuel : analyse interactionnelle

Author

Traverso, Véronique, Traverso, Véronique, Détienne F., Traverso V., Interactions, Corpus, Apprentissages, Représentations (ICAR), École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS), and École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS)

Subjects
linguistique interactionnelle, ergonomie cognitive, [SHS.LANGUE]Humanities and Social Sciences/Linguistics, confrontation méthodologique, [SHS.LANGUE] Humanities and Social Sciences/Linguistics, multimodalité
Published
2009

32. Les interactions en site commercial : présentation

Author

Kerbrat-Orecchioni, Catherine, Traverso, Véronique, Traverso, Véronique, Kerbrat-Orecchioni C, Traverso V., Interactions, Corpus, Apprentissages, Représentations (ICAR), École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS), and École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS)

Subjects
état de la question, interaction de service, [SHS.LANGUE]Humanities and Social Sciences/Linguistics, interactions de travail, interactions commerciales, [SHS.LANGUE] Humanities and Social Sciences/Linguistics
Published
2009

33. Les activités transactionnelles et les autres : l'hétérogénéité de l'interaction en site commercial

Author

Doury, Marianne, Traverso, Véronique, Traverso, Véronique, Kerbrat-Orecchioni C, Traverso V., Interactions, Corpus, Apprentissages, Représentations (ICAR), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS), and École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS)

Subjects
échanges à bâtons rompus, activités interactionnelles, plaisanteries, [SHS.LANGUE]Humanities and Social Sciences/Linguistics, [SHS.LANGUE] Humanities and Social Sciences/Linguistics
Published
2009

34. Cadres, espaces, objets et multimodalité dans l'interaction en site commercial

Author

Traverso, Véronique, Traverso, Véronique, Kerbrat-Orecchioni C, Traverso V., Interactions, Corpus, Apprentissages, Représentations (ICAR), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS), and École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS)

Subjects
activité, interaction, cadres participatifs, [SHS.LANGUE]Humanities and Social Sciences/Linguistics, [SHS.LANGUE] Humanities and Social Sciences/Linguistics, multimodalité
Published
2009

35. Principes de transcription

Author

Détienne, Françoise, Greco, Lucas, Mondada, Lorenza, Traverso, Véronique, Visser, Willemien, Laboratoire Traitement et Communication de l'Information (LTCI), Télécom ParisTech-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS), RFC - Recherche sur le français contemporain - EA 1483 (RFC), Université Sorbonne Nouvelle - Paris 3, Interactions, Corpus, Apprentissages, Représentations (ICAR), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS), Détienne, F. - Traverso, V., Mondada, Lorenza, Détienne, F. - Traverso, V., and École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.LANGUE]Humanities and Social Sciences/Linguistics, [SHS.LANGUE] Humanities and Social Sciences/Linguistics, linguistique
Published
2009

36. Introduction

Author

Détienne, Françoise, Traverso, Véronique, Traverso, Véronique, and Détienne F. et Traverso V.

Subjects
[SHS.LANGUE] Humanities and Social Sciences/Linguistics, ComputingMilieux_MISCELLANEOUS, linguistique
Published
2008

37. Les interactions en site commercial : des interactions « polies »

Author

Kerbrat-Orecchioni, Catherine, Lascar, Justine, Kerbrat-Orecchioni, C. et Traverso V., Interactions, Corpus, Apprentissages, Représentations (ICAR), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS), and École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SHS.LANGUE]Humanities and Social Sciences/Linguistics, [SHS.LANGUE] Humanities and Social Sciences/Linguistics, linguistique
Published
2008

38. Confidence/dévoilement de soi dans l'interaction : introduction

Author

Kerbrat-Orecchioni, Catherine, Traverso, Véronique, Interactions, Corpus, Apprentissages, Représentations (ICAR), École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS), Kerbrat-Orecchioni C, Traverso V., Traverso, Véronique, and École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-INRP-Ecole Normale Supérieure Lettres et Sciences Humaines (ENS LSH)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Interaction, dévoilement, séquence, fiction, [SHS.LANGUE]Humanities and Social Sciences/Linguistics, [SHS.LANGUE] Humanities and Social Sciences/Linguistics
Published
2007

39. Les traces de l'émotion chez Galilée, docere et movere

Author

Bonnet, Valérie, Laboratoire d'Etudes et de Recherches Appliquées en Sciences Sociales (LERASS), Université Paul-Valéry - Montpellier 3 (UPVM)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, and Plantin Ch., Doury M. & Traverso V. (éds.).

Subjects
[SHS.HISPHILSO]Humanities and Social Sciences/History, Philosophy and Sociology of Sciences, argumentation, rhétorique, [SHS.LANGUE]Humanities and Social Sciences/Linguistics
Abstract

CD-Rom joint à l'ouvrage; International audience; Cet article vise à rechercher les traces de l’émotion dans un discours réputé à dominante référentielle : le discours scientifique. Le nombre important d’expressions marquant les émotions de l’auteur nous montre que les règles du genre ont évolué depuis le 17e siècle. La présence de ces expressions semble due au caractère nouveau du genre comme de la méthode, l’auteur ne pouvant emprunter les méthodes habituelles comme la preuve, le témoignage, la démonstration. Il utilise alors les seuls moyens que lui autorise sa position d’observateur : la fonction émotive, espérant gagner la confiance (fides) du lecteur en lui faisant partager ses émotions.

Published
2000

40. Patients' Disalignment in Two Different Healthcare Settings.

Author

Niemants N, Ticca AC, and Traverso V

Subjects
France, Humans, Italy, Communication, Physician-Patient Relations
Abstract

Among the important bulk of research devoted to medical consultations, one recurrently discussed issue has been that of patients' alignment with practitioners' recommendations. If this question has not always been formulated in terms of alignment, all the studied cases deal with how patients comply, or not, with practitioners' first actions. They show that social actions such as suggestions, proposals, offers, etc. are not unilaterally offered by practitioners to patients, but frequently discussed and negotiated. This may result in patients being more willing to comply with jointly achieved solutions. In this paper, we will fill in some more details of this picture by focusing on interactional resources used by patients to show their disalignment toward less investigated types of first actions (i.e. non-medication recommendations, home remedies, proposals or suggestions to accomplish certain activities), thereby acknowledging the central role played by patients in two different healthcare settings (general and mental health). We will also compare how linguistic and cultural diversity are handled when patients and practitioners communicate directly as well as when communication is interpreter-mediated, thereby problematizing the presence of an interpreter who needs to grasp the variety of resources used by patients in order to render both their disalignment and practitioners' responses to it. Taking into account audio- and video-recorded naturally occurring data collected in Italy and France, we will additionally show the relevance of multimodal analysis for a better understanding of the resources involved, as well as of the dynamics of interpreter-mediated communication in healthcare.

Published
2021
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41. Knowledge of gestational alcohol drinking risks: results from a survey among Italian secondary school students.

Author

Berretta P, Minutillo A, Pichini S, Traverso V, Toth G, Scaravelli G, De Luca R, and Da Rin Della Mora R

Subjects
Adolescent, Cross-Sectional Studies, Female, Fetal Alcohol Spectrum Disorders, Health Knowledge, Attitudes, Practice, Humans, Italy, Male, Risk-Taking, Schools, Sex Factors, Students, Surveys and Questionnaires, Alcohol Drinking adverse effects, Pregnancy
Abstract

Introduction: Ethanol is the most widely used drug worldwide. Its consumption has been increasing, and it is reported even during childbearing. Prenatal exposure to ethanol can lead to irreversible damages of the fetus. Knowledge about this risk could prevent these damages. There is no information about knowledge of the Italian students on this issue., Methods: Therefore the aim of this study was to describe the awareness of the Italian students attending the last year of secondary school about risk of gestational alcohol drinking for the delivering mother and the fetus. An online multiple-choice anonymous survey for students was used and e-mailed to the all Italian secondary schools., Results: The respondents were 9.921 and the obtained results evidenced that that young females are more informed than males, and students in Northern and Central Italy are better informed than those in the South, especially on general aspects. The most of respondents knows that alcohol consumption during pregnancy can damage the fetus. However, many youngsters failed to translate this belief into the practice., Conclusions: In conclusion, interventions are needed to enhance knowledge and prevent these damages, and health professionals, with nurses in the first row are entitled to provide education on this topic.

Published
2018
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42. [Survey of secondary school students 18 years old or older about knowledge on risks to the fetus related to alcohol consumption during pregnancy].

Author

Traverso V and Da Rin Della Mora R

Subjects
Adolescent, Female, Health Personnel, Humans, Male, Pregnancy, Risk, Self Report, Alcohol Drinking adverse effects, Fetal Alcohol Spectrum Disorders etiology, Health Knowledge, Attitudes, Practice
Abstract

Introduction:  Being not perceived as risky for health, alcohol consumption is socially accepted worldwide. Nevertheless, related risks for the fetus are relevant, even for occasional use. In Italy there is a high consumption of alcohol among young people and pregnant women, and an increasing number of children born with alcohol-related disorders., Aim: To investigate the knowledge on fetal alcohol-related disorders among students over 18 years of age in a regional capital in the North of Italy., Methods: In May, 2015, a survey with an online, validated questionnaire was conducted on N=1321 students., Results:  A total of N=246 valid questionnaires was obtained (18,6%). The 76,4% of students knew that drinking alcohol during pregnancy damages fetus, but only the 23,5% knew that this damages are permanent. Only the 43,5% knew that this damages are preventable; of them, the 77,6% stated that the only way to prevent them is abstention from alcohol consumption, though 30,1% answered that alcohol use is allowed without damage the fetus. Only the 17,5% answered to know Fetal Alcohol Spectrum Disorders., Conclusion:  The study showed satisfying knowledge on general aspects, but scarce on specific ones, and a limited awareness that only complete abstinence from alcohol when pregnant can prevent irreversible damages of the fetus. This results could help to program educational interventions for teenagers, carried out by nurses, pediatric nurses and other health professionals to increase their knowledge and prevent in utero exposure. Future studies could be aimed at identifying the most effective educational methods to do so.

Published
2017
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43. Gait changes with the use of Heelys: a case study.

Author

Norem N, Feuerstein C, Traverso V, Zomaya N, Crews R, and Wrobel JS

Subjects
Biomechanical Phenomena, Equipment Design, Exercise Test, Humans, Male, Young Adult, Forefoot, Human physiopathology, Gait physiology, Heel physiology, Orthotic Devices, Shoes
Abstract

Heelys shoes are a novel athletic shoe with a concealed wheel. They have been popular among youths since their introduction in 2000. This case study serves as a first look into the biomechanical implications of Heelys shoes on gait. Pressure readings of the forefoot, midfoot, and rearfoot during ambulation in regular athletic-shoe walking, Heelys without the wheel walking, Heelys with the wheel walking, and Heelys skating with the wheel were recorded on a single subject using the Pedar X System. A visual gait analysis was also performed on the subject. The resulting data show increased forefoot and rearfoot pressure while walking with the Heelys with the wheel. The visual gait analysis showed a diminished heel strike and a more rapid forefoot loading. These results demonstrate that Heelys do in fact affect the biomechanics of gait.

Published
2009
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44. [Situational communication practices: objectives and methods of interaction analysis].

Author

Traverso V

Subjects
Attitude of Health Personnel, Attitude to Health, Data Interpretation, Statistical, Female, Health Knowledge, Attitudes, Practice, Humans, Negotiating psychology, Nurse's Role psychology, Nurse-Patient Relations, Office Visits, Organizational Objectives, Patient Education as Topic, Postnatal Care psychology, Psycholinguistics, Research Design, Semantics, Communication, Nursing Methodology Research methods, Physician's Role psychology, Physician-Patient Relations, Sociology, Medical methods
Published
2007

45. Phosphoinositides, ezrin/moesin, and rac1 regulate fusion of rhodopsin transport carriers in retinal photoreceptors.

Author

Deretic D, Traverso V, Parkins N, Jackson F, Rodriguez de Turco EB, and Ransom N

Subjects
Actins metabolism, Animals, Cytoskeletal Proteins, Cytosol metabolism, Enzyme Activation drug effects, Lysophospholipids pharmacology, Mass Spectrometry, Membrane Fusion physiology, Microscopy, Confocal, Microscopy, Electron, Mutation, Phosphatidate Phosphatase drug effects, Phosphatidate Phosphatase metabolism, Phospholipase D drug effects, Phospholipase D metabolism, Propranolol pharmacology, Protein Transport physiology, Rod Cell Outer Segment, Sphingosine analogs & derivatives, Sphingosine pharmacology, rab GTP-Binding Proteins, Microfilament Proteins metabolism, Phosphatidylinositols metabolism, Phosphoproteins metabolism, Rhodopsin metabolism, rac1 GTP-Binding Protein metabolism
Abstract

The post-Golgi trafficking of rhodopsin in photoreceptor cells is mediated by rhodopsin-bearing transport carriers (RTCs) and regulated by the small GTPase rab8. In this work, we took a combined pharmacological-proteomic approach to uncover new regulators of RTC trafficking toward the specialized light-sensitive organelle, the rod outer segment (ROS). We perturbed phospholipid synthesis by activating phospholipase D with sphingosine 1-phosphate (S1P) or inhibiting phosphatidic acid phosphohydrolase by propranolol (Ppl). S1P stimulated the overall rate of membrane trafficking toward the ROS. Ppl stimulated budding of RTCs, but blocked membrane delivery to the ROS. Ppl caused accumulation of RTCs in the vicinity of the fusion sites, suggesting a defect in tethering, similar to the previously described phenotype of the rab8T22N mutant. Proteomic analysis of RTCs accumulated upon Ppl treatment showed a significant decrease in phosphatidylinositol-4,5-bisphosphate-binding proteins ezrin and/or moesin. Ppl induced redistribution of moesin, actin and the small GTPase rac1 from RTCs into the cytosol. By confocal microscopy, ezrin/moesin and rac1 colocalized with rab8 on RTCs at the sites of their fusion with the plasma membrane; however, this distribution was lost upon Ppl treatment. Our data suggest that in photoreceptors phosphatidylinositol-4,5-bisphosphate, moesin, actin, and rac1 act in concert with rab8 to regulate tethering and fusion of RTCs. Consequentially, they are necessary for rhodopsin-laden membrane delivery to the ROS, thus controlling the critical steps in the biogenesis of the light-detecting organelle.

Published
2004
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46. Basic fibroblast and epidermal growth factors stimulate survival in adult porcine photoreceptor cell cultures.

Author

Traverso V, Kinkl N, Grimm L, Sahel J, and Hicks D

Subjects
Animals, Arrestin metabolism, Blotting, Western, Calcium-Binding Proteins metabolism, Cell Culture Techniques, Cell Survival drug effects, Cytoprotection drug effects, Dose-Response Relationship, Drug, Drug Combinations, Fluorescent Antibody Technique, Indirect, Hippocalcin, Phosphopyruvate Hydratase metabolism, Phosphorylation, Photoreceptor Cells, Vertebrate metabolism, Recoverin, Rod Opsins metabolism, Swine, Tyrosine metabolism, Epidermal Growth Factor pharmacology, Eye Proteins, Fibroblast Growth Factor 2 pharmacology, Lipoproteins, Nerve Tissue Proteins, Photoreceptor Cells, Vertebrate cytology
Abstract

Purpose: To investigate the effects of basic fibroblast and epidermal growth factor (FGF2 and EGF, respectively) on the survival and phenotypic expression of photoreceptors isolated from adult mammalian retinas., Methods: Primary cultures highly enriched in photoreceptors were prepared from adult domestic pig retinas and maintained in chemically defined medium. Cell culture composition was characterized through the use of specific antibody markers of retinal neurons, and neuronal survival was quantified through viability assays as a function of time in the presence or absence of different doses of FGF2 and EGF. Western blot analysis of phosphotyrosine residues was used to monitor activation of FGF2 and EGF signaling pathways., Results: Reproducible survival of adult pig rod and cone photoreceptors was obtained for approximately 2 weeks in vitro, with the continued expression of rod opsin, recoverin, S-antigen, cone arrestin, and neuron-specific enolase. Purity of cultures was routinely more than 95% photoreceptors, with a rod-cone ratio of 2:3.1. Photoreceptor survival was stable for the initial week, decreasing slowly during the second, with rapid cell loss occurring thereafter. In the presence of FGF2 (20 ng/mL), the percentage of photoreceptor survival during the second week in culture was statistically significantly different, at least two times higher than in control experiments. Photoreceptor survival correlated directly with increasing concentrations of FGF2, and also of EGF. Combined treatment with FGF2 and EGF did not induce higher survival than either factor alone. There was no detectable selective loss of rods or cones in the experimental model. Phosphotyrosine immunoblots after stimulation of cultures with FGF2 and EGF revealed time-dependent appearance of multiple immunoreactive bands., Conclusions: The adult pig photoreceptor culture in the current study exhibits reproducible neuronal survival in vitro and represents a useful novel experimental system for the study of potential neuroprotective effects and signaling pathways of neurotrophic factors such as FGF2 and EGF in fully adult higher mammalian retina.

Published
2003
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47. Retinal cAMP levels during the progression of retinal degeneration in rhodopsin P23H and S334ter transgenic rats.

Author

Traverso V, Bush RA, Sieving PA, and Deretic D

Subjects
Animals, Animals, Genetically Modified, Cell Count, Dark Adaptation, Disease Progression, Female, Immunohistochemistry, Male, Photoreceptor Cells, Vertebrate pathology, Rats, Rats, Sprague-Dawley, Retinal Degeneration pathology, Cyclic AMP metabolism, Mutation, Photoreceptor Cells, Vertebrate metabolism, Retinal Degeneration genetics, Retinal Degeneration metabolism, Rhodopsin genetics
Abstract

Purpose: To test whether high levels of cAMP promote apoptosis and shorten the life of retinal rod photoreceptors, the changes in cAMP levels during retinal degeneration were analyzed in two transgenic rat models that express rhodopsin P23H and S334ter mutations., Methods: Dark- and light-adapted heterozygous P23H (lines 1 and 3; P23H-1 and -3), S334ter line 4 (S334ter-4), and Sprague-Dawley (control) rats were studied at 4 to 8 weeks by cAMP enzyme competitive immunoassay and by cAMP immunocytochemistry., Results: In control animals retinal cAMP content reached a steady state level at 30 days of age. Dark-adapted control retinas had up to 97% higher cAMP content than light-adapted retinas, and photoreceptor cells were the major source of this increase. Dark-adapted photoreceptors in all three lines of transgenic rats at advanced stages of retinal degeneration had cAMP content different from that of the control. In rats that express mutant rhodopsin, the number of photoreceptor cells was progressively reduced, because of retinal degeneration, but dark-adapted cAMP levels did not decline accordingly. P23H transgenic animals of both lines had higher levels of cAMP per photoreceptor cell count than control animals. This elevation was more pronounced as degeneration progressed. S334ter animals showed smaller cAMP elevation than P23H rats at a similar stage of retinal degeneration, but at a point when S334ter rats were undergoing rapid retinal degeneration, whereas in P23H rats retinal degeneration was slowing down., Conclusions: All three lines of transgenic rats carrying rhodopsin mutations show an increase in dark-adapted photoreceptor cAMP levels. A complex relationship exists between cAMP levels and the rate of cell death in the retina. Although initially higher levels of cAMP may promote cell survival and slow down retinal degeneration, ultimately, elevated cAMP levels may become toxic and may contribute to retinal cell death.

Published
2002

48. Towards the proteome of the rhodopsin-bearing post-Golgi compartment of retinal photoreceptor cells.

Author

Morel V, Poschet R, Traverso V, and Deretic D

Subjects
Amino Acid Sequence, Animals, Cell Compartmentation, Electrophoresis, Gel, Two-Dimensional, Mass Spectrometry, Microscopy, Confocal, Molecular Sequence Data, Ranidae, Retina cytology, Golgi Apparatus metabolism, Photoreceptor Cells metabolism, Proteome, Retina metabolism, Rhodopsin metabolism
Abstract

Polarized sorting of rhodopsin in retinal rod photoreceptor cells is mediated by post-Golgi carrier membranes that bud from the trans-Golgi network and fuse with the specialized domain of the plasma membrane in the rod inner segment. The identity of the majority of the resident proteins of this organelle still remains elusive, despite multifaceted approaches to study this compartment. In the present study we have taken a proteomic approach to the analysis of the post-Golgi carriers. First, we modified the previously established fractionation protocols in order to achieve greater purity of the isolated membranes. Specifically, the new fractionation scheme depleted the post-Golgi fraction of cytosolic proteins that were the most abundant contaminants complicating analysis of two-dimensional (2-D) gel profiles in our previous preparations. The isolated membranes were subjected to 2-D gel electrophoresis, immunoblotting and microsequencing. This analysis showed that the improved subcellular fractionation yielded a fraction highly enriched in rhodopsin-bearing post-Golgi carrier membranes. Two-dimensional mapping revealed 29 proteins that are preferentially found in this fraction and therefore represent candidates for post-Golgi membrane-specific proteins. This preparation of rhodopsin-bearing post-Golgi carriers is a first step towards the proteomics of this important organelle.

Published
2000
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49. Cellular and subcellular localization of annexin IV in rabbit intestinal epithelium, pancreas and liver.

Author

Massey D, Traverso V, Rigal A, and Maroux S

Subjects
Animals, Annexins, Epithelium chemistry, Fluorescent Antibody Technique, Immunoblotting, Rabbits, Intestines chemistry, Liver chemistry, Pancreas chemistry, Pregnancy Proteins analysis
Abstract

The results of immunoblot analysis performed with a specific monoclonal antibody showed that the intestinal mucosa, pancreas and liver are privileged tissues for the expression of annexin IV. Immunofluorescence labelling of thin frozen sections of these tissues showed a strong concentration of annexin IV along the basolateral domain of the plasma membrane of intestinal absorbing cells, hepatocytes and pancreatic acinar cells, whereas in intestinal mucous secreting cells and centro acinar pancreatic cells, annexin IV was found to be present throughout the cytoplasm.

Published
1991
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