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8 results on '"Traore, Tahirou"'

4. Cloning, over-expression, and characterization of a new carboxypeptidase A gene of Bacillus pumilus ML413 in Bacillus subtilis 168

Author

Traore Tahirou, Yang Taowei, Meijuan Xu, Xian Zhang, Genchu Huang, Junping Zhou, and Zhiming Rao

Subjects
0301 basic medicine, Bacillus subtilis, Applied Microbiology and Biotechnology, Carboxypeptidase activity, 03 medical and health sciences, Shuttle vector, Genetics, Molecular Biology, chemistry.chemical_classification, 030102 biochemistry & molecular biology, biology, Bacillus pumilus, fungi, Exopeptidase, biology.organism_classification, Molecular biology, Enzyme assay, Bacillus pumilus, Bacillus subtilis 168, over-expression, orthogonal arrays, carboxypeptidase A, metallocarboxypeptidase, 030104 developmental biology, Enzyme, Biochemistry, chemistry, biology.protein, Carboxypeptidase A, Agronomy and Crop Science, Biotechnology
Abstract

Carboxypeptidase A (CPAs) are a well-studied group of zinc-containing exopeptidases that facilitate the breakdown of proteins and peptides during metabolism. Carboxypeptidase A is typically produced in mammalian pancreatic, brain and other tissues. A new gene encoding carboxypeptidase A in the prokaryote Bacillus pumilus was amplified by polymerase chain reaction (PCR), ligated into the shuttle vector pMA5, and cloned in a GRAS bacteria-Bacillus subtilis 168 host. This gene sequence contained a 1621 bp open reading frame that encodes a protein of 540 amino acids. The optimum pH and temperature for enzyme activity were 7.5 and 50°C, respectively. The enzyme was quite stable at neutral pH and maintained about 65% activity following a 24 h incubation at 40°C. The Km of this CPA was 0.1 mM, much higher than in mammalian species. Glycerol, ammonium sulfate, and sodium citrate improved enzyme activity under optimal culture condition. The carboxypeptidase activity in recombinant B. subtilis 168 reached a maximum of 179 U ml-1 in a 5 L fermentator when cultured on improved medium. The over expression of carboxypeptidase A in Bacillus subtilis has commercial applications. Key words : Bacillus pumilus, Bacillus subtilis 168, over-expression, orthogonal arrays, carboxypeptidase A, metallocarboxypeptidase.

Published
2016

5. Effects of energy density and sweetness of gruels on Burkinabe infant energy intakes in free living conditions

Author

Vieu, Marie-Catherine, Traore, Tahirou, and Treche, Serge

Subjects
Infants -- Food and nutrition, Sucrose -- Physiological aspects, Bioenergetics -- Research, Food/cooking/nutrition
Abstract

In free living conditions, 24 breastfed infants, aged 6 to 10 months, were given successively five experimental gruels to study the effect of energy density (ED) and sweetness (sweet taste) on energy intakes (EI). Four gruels (G0, G1, G9 and G20) were prepared with experimental flours which were composed of the same local ingredients and which contained different levels of sucrose. The fifth gruel (GC) was prepared with an industrial flour. G0 had an average ED of 45 kcal/100g (189 kJ/100 g) and the other gruels an average ED of 110 kcal/100g (461 kJ/100 g). Although the sugar contents of the flours were 1% for G1, 9% for G0 and G9 and 20% for G20, because of flour composition and gruel dry matter content, the gruel G1 had the same sweetness as G0, G20 the same sweetness as GC and G9 a sweetness between that of G1 and G20. The results show that the amounts of G0 consumed were significantly higher than those of high ED gruels (7.84 for G0 vs 6.12, 5.63, 4.46, 4.72 g/kg body weight/meal, respectively for G20, G9, G1 and GC, P [is less than] 0.05). However, EI from high ED gruels were significantly (P [is less than] 0.001) higher than those from G0 (6.65, 6.10, 4.86, 4.83 kcal/kg/meal, respectively for G20, G9, G1 and GC vs 3.46 for G0). Energy intakes from G9 and G20 gruels were not significantly different but were significantly higher than those from GC and G1 (P [is less than] 0.001). So, consumption of sweet gruels with high ED and composed of local ingredients increased, at least by 76%, the EI from gruels in comparison with those from low ED gruels, but the amounts consumed by the infants remained too low to cover more than 15% of their daily total energy needs.

Published
2001

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