1. A double-negative feedback loop mediated by non-coding RNAs contributes to tooth morphogenesis.
- Author
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Sun M, Li N, Zhang W, Li A, and Li Y
- Subjects
- Animals, Swine, Apoptosis genetics, RNA, Long Noncoding genetics, RNA, Long Noncoding metabolism, Gene Expression Regulation, Developmental, Odontogenesis genetics, Swine, Miniature, Morphogenesis genetics, Tooth metabolism, MicroRNAs genetics, MicroRNAs metabolism, Feedback, Physiological
- Abstract
Tooth morphogenesis is a critically ordered process manipulated by a range of signaling factors. Particularly, the involvement of fine-tuned signaling mediated by non-coding RNAs has been of longstanding interest. Here, we revealed a double-negative feedback loop acted by a long non-coding RNA (LOC102159588) and a microRNA (miR-133b) that modulated tooth morphogenesis of miniature swine. Mechanistically, miR-133b repressed the transcription of LOC102159588 through downstream target Sp1. Conversely, LOC102159588 not only inhibited the transport of pre-miR-133b from the nucleus to the cytoplasm by regulating exportin-5 but also served as a sponge in the cytoplasm, suppressing functional miR-133b. Together, the double-negative feedback loop maintained normal tooth morphogenesis by modulating endogenous apoptosis. Related disruptions would lead to an arrest of tooth development and may result in tooth malformations., Competing Interests: Declaration of competing interest All authors disclosed no conflict of interest to competing or financial of this article., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
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