1. Assessment of cell cycle arrest biomarkers and neutrophil gelatinase-associated lipocalin to distinguish acute kidney injury from other diseases in dogs.
- Author
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Biscop A, Castelain D, Stock E, Demeyere K, Meyer E, Devriendt N, Dorn E, De Laet N, and Paepe D
- Subjects
- Animals, Dogs, Male, Female, Prospective Studies, Renal Insufficiency, Chronic veterinary, Renal Insufficiency, Chronic urine, Renal Insufficiency, Chronic diagnosis, Renal Insufficiency, Chronic blood, Critical Illness, Cell Cycle Checkpoints, Diagnosis, Differential, Acute-Phase Proteins, Proto-Oncogene Proteins, Lipocalins, Dog Diseases urine, Dog Diseases diagnosis, Dog Diseases blood, Acute Kidney Injury veterinary, Acute Kidney Injury diagnosis, Acute Kidney Injury urine, Acute Kidney Injury blood, Biomarkers blood, Biomarkers urine, Tissue Inhibitor of Metalloproteinase-2 urine, Tissue Inhibitor of Metalloproteinase-2 blood, Insulin-Like Growth Factor Binding Proteins urine, Insulin-Like Growth Factor Binding Proteins blood, Lipocalin-2 urine, Lipocalin-2 blood
- Abstract
Background: Cell cycle arrest biomarkers (tissue inhibitor of metalloproteinase-2 [uTIMP-2] and insulin-like growth factor binding protein 7 [uIGFBP7]), and neutrophil gelatinase-associated lipocalin (NGAL) variables are valuable biomarkers for early diagnosis of acute kidney injury (AKI) in people., Objectives: To evaluate uTIMP-2, uIGFBP7, fractional excretion of NGAL (FeNGAL), and urinary to serum NGAL ratio (u/sNGAL) in healthy dogs, dogs with AKI, dogs with chronic kidney disease (CKD), and critically ill (CI) dogs., Animals: Forty-two client-owned dogs (healthy, n = 10; AKI, n = 11; CKD, n = 11; CI, n = 10)., Methods: Prospective, observational study. After assessment of routine renal biomarkers, stress (uTIMP-2, uIGFBP7) and damage (NGAL) biomarkers were measured, using ELISA kits, and normalized to urinary creatinine (uCr)., Results: Normalized uTIMP-2 and [uTIMP-2] × [uIGFBP7]/uCr were significantly higher in the AKI group (median 151.9 [range, 2.2-534.2] and 62.9 [1.1-266.8] pg/mL respectively), compared to healthy dogs (0.3 [0.2-74.7]; P < .001 and 0.16 [0.1-58.1] pg/mL; P < .001), dogs with CKD (0.7 [0.3-742.5]; P = .04 and 0.37 [0.2-180.1] pg/mL; P = .03) and CI dogs (1.9 [0.2-37.0]; P = .03 and 0.8 [0.1-16.1] pg/mL; P = .02). Fractional excretion of NGAL was significantly higher in dogs with AKI (54.17 [7.93-155.32] %), than in healthy (0.03 [0.01-0.21] %; P < .001) and CI dogs (3.05 [0.05-28.86] %; P = .02)., Conclusions and Clinical Importance: Normalized uTIMP-2, [uTIMP-2] × [uIGFBP7]/uCr, and FeNGAL can be valuable renal biomarkers for early diagnosis of AKI in dogs., (© 2024 The Author(s). Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.)
- Published
- 2024
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