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A study was done on the effects of polyunsaturated fatty acids on postnatal uterine development. It was shown that dietary alpha linolenic acid exert a significant effect on the phospholipid fatty acid composition of rat uterus. It was further shown that feeding rats a purified diet deficient in alpha linolenic acid for three generations significantly affected early sexual development especially the ponderant weight of the growing uterus.

The effects of various biological detergents on the particulate cGMP-stimulated cAMP phosphodiesterase activity from rat heart were investigated. When added to particulate fractions, anionic and non-ionic detergents diversely increased both cAMP and cGMP phosphodiesterase activities and slightly decreased the stimulatory effect of cGMP on cAMP hydrolysis whereas cationic detergents were rather inhibitory and drastically lowered the stimulatory effect of cGMP. Among the most efficient detergents, only sodium cholate was able to solubilize phosphodiesterase activity and preserve the stimulatory effect of cGMP on cAMP hydrolysis. Furthermore, the addition of glycerol significantly improved the conservation of the allosteric properties of the enzyme. Kinetic properties of the cholate-solubilized phosphodiesterase were quite identical to those of the membrane-bound enzyme.