1. Tumour catabolism independent of malnutrition and inflammation in upper GI cancer patients revealed by longitudinal metabolomics
- Author
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Janusz vonRenesse, Felix vonBechtolsheim, Sophie Jonas, Lena Seifert, Tiago C. Alves, Adrian M. Seifert, Filip Komorek, Guergana Tritchkova, Mario Menschikowski, Ulrich Bork, Ronny Meisterfeld, Marius Distler, Triantafyllos Chavakis, Jürgen Weitz, Alexander M. Funk, Christoph Kahlert, and Peter Mirtschink
- Subjects
NMR ,Gastrointestinal cancer ,Metabolic profile ,Metabolome ,Ketone bodies ,3‐Hydroxybutyrate ,Diseases of the musculoskeletal system ,RC925-935 ,Human anatomy ,QM1-695 - Abstract
Abstract Background The detrimental impact of malnutrition and cachexia in cancer patients subjected to surgical resection is well established. However, how systemic and local metabolic alterations in cancer patients impact the serum metabolite signature, thereby leading to cancer‐specific differences, is poorly defined. In order to implement metabolomics as a potential tool in clinical diagnostics and disease follow‐up, targeted metabolite profiling based on quantitative measurements is essential. We hypothesized that the quantitative metabolic profile assessed by 1H nuclear magnetic resonance (NMR) spectroscopy can be used to identify cancer‐induced catabolism and potentially distinguish between specific tumour entities. Importantly, to prove tumour dependency and assess metabolic normalization, we additionally analysed the metabolome of patients' sera longitudinally post‐surgery in order to assess metabolic normalization. Methods Forty two metabolites in sera of patients with tumour entities known to cause malnutrition and cachexia, namely, upper gastrointestinal cancer and pancreatic cancer, as well as sera of healthy controls, were quantified by 1H NMR spectroscopy. Results Comparing serum metabolites of patients with gastrointestinal cancer with healthy controls and pancreatic cancer patients, we identified at least 15 significantly changed metabolites in each comparison. Principal component and pathway analysis tools showed a catabolic signature in preoperative upper gastrointestinal cancer patients. The most specifically upregulated metabolite group in gastrointestinal cancer patients was ketone bodies (3‐hydroxybutyrate, P
- Published
- 2023
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