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Effect of aging on muscle mitochondrial substrate utilization in humans
- Source :
- Proceedings of the National Academy of Sciences. 112:11330-11334
- Publication Year :
- 2015
- Publisher :
- Proceedings of the National Academy of Sciences, 2015.
-
Abstract
- Previous studies have implicated age-associated reductions in mitochondrial oxidative phosphorylation activity in skeletal muscle as a predisposing factor for intramyocellular lipid (IMCL) accumulation and muscle insulin resistance (IR) in the elderly. To further investigate potential alterations in muscle mitochondrial function associated with aging, we assessed basal and insulin-stimulated rates of muscle pyruvate dehydrogenase (VPDH) flux relative to citrate synthase flux (VCS) in healthy lean, elderly subjects and healthy young body mass index- and activity-matched subjects. VPDH/VCS flux was assessed from the (13)C incorporation from of infused [1-13C] glucose into glutamate [4-13C] relative to alanine [3-13C] assessed by LC-tandem MS in muscle biopsies. Insulin-stimulated rates of muscle glucose uptake were reduced by 25% (P
- Subjects :
- Adult
Blood Glucose
Aging
medicine.medical_specialty
Magnetic Resonance Spectroscopy
Glucose uptake
medicine.medical_treatment
Pyruvate Dehydrogenase Complex
Citrate (si)-Synthase
Mitochondrion
Corrections
Insulin resistance
Tandem Mass Spectrometry
Internal medicine
medicine
Humans
Hypoglycemic Agents
Insulin
Citrate synthase
Muscle, Skeletal
Aged
Carbon Isotopes
Multidisciplinary
biology
Skeletal muscle
Biological Sciences
Glucose clamp technique
Lipid Metabolism
medicine.disease
Pyruvate dehydrogenase complex
Mitochondria
Glucose
Endocrinology
medicine.anatomical_structure
Glucose Clamp Technique
biology.protein
Oxidation-Reduction
Chromatography, Liquid
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....ffbe3e467c3ed88d07ca82a17bece250